species-specific functions of epstein-barr virus nuclear antigen 2 (ebna2) reveal dual roles for initiation and maintenance of b cell immortalization.epstein-barr virus (ebv) and related lymphocryptoviruses (lcv) from non-human primates infect b cells, transform their growth to facilitate life-long viral persistence in the host, and contribute to b cell oncogenesis. co-evolution of lcv with their primate hosts has led to species-specificity so that lcvs preferentially immortalize b cells from their natural host in vitro. we investigated whether the master regulator of transcription, ebv nuclear antigen 2 (ebna2), is involved in lcv species-sp ...201729261800
whole transcriptome profiling reveals major cell types in the cellular immune response against acute and chronic active epstein-barr virus infection.epstein-barr virus (ebv) is a common human pathogen that infects over 95% of the population worldwide. in the present study, the whole transcriptome microarray data were generated from peripheral blood mononuclear cells from chinese children with acute infectious mononucleosis (aim) and chronic active ebv infection (caebv) that were also compared with a publicly available microarray dataset from a study of american college students with aim. our study characterized for the first time a broad spe ...201729259291
identification of t-cell receptor expression in ebv-positive neoplastic cells in extranodal nk/t-cell lymphoma, nasal-type and comparison with t-cell receptor gene rearrangement by biomed-2 assay.the cellular lineage of extranodal nk/t-cell lymphoma, nasal-type (enktl) is determined by expression of t-cell receptor (tr) or tr gene rearrangement. in enktl, from tr immunohistochemistry it may often be difficult to decide whether tr-positive cells are tumor cells or not, especially when tr is expressed in a subset of tumor cells. to analyze tr expression pattern and tr rearrangement in t-lineage enktl, we performed double immunofluorescence staining for epstein-barr virus-encoded small rnas ...201729258904
coinfection with epstein-barr virus (ebv), human papilloma virus (hpv) and polyoma bk virus (bkpyv) in laryngeal, oropharyngeal and oral cavity cancer.most research providing evidence for the role of oncogenic viruses in head and neck squamous cell carcinoma (scc) development is focused on one type of virus without analyzing possible interactions between two or more types of viruses. the aim of this study was to analyse the prevalence of co-infection with human papillomavirus (hpv), epstein-barr virus (ebv) and polyoma bk virus (bkpyv) in oral, oropharyngeal and laryngeal squamous cell carcinomas in polish patients. the correlations between vi ...201729257122
human papillomavirus (hpv)-associated lymphoepithelioma-like carcinoma of the vagina and anal canal: a rare variant of squamous cell carcinoma.lymphoepithelioma-like carcinoma (lelc) is an uncommon variant of squamous cell carcinoma, which is histologically identical to lymphoepithelial carcinoma of the nasopharynx. lelcs have been reported at a variety of sites, including the stomach, salivary gland, thymus, cervix, endometrium, breast, skin, bladder, and lung. we report 2 lelcs of the vagina and 1 of the anal canal, the first report of lelc at the latter site. all 3 neoplasms were diffusely positive with p16 (block-type immunoreactiv ...201729257037
a rare cause of gastrointestinal bleeding in the post-transplant lymphoproliferative disorder (ptld) is a life-threatening complication noted after solid organ transplantation and is frequently related to epstein-barr virus infection. the present case highlights an unusual presentation of ptld - gastrointestinal bleeding - in the absence of systemic symptoms.201729255810
hodgkin's lymphoma in crohn's disease treated with infliximab.lymphoproliferative disorders, particularly non-hodgkin's and hodgkin's lymphomas, are rare in patients with inflammatory bowel diseases. the use of thiopurines and infection by epstein-barr virus are well-known cofactors that can raise its prevalence. other risk factors such as disease activity and biological treatment are the subject of discussion, without enough data in the literature to confirm a potential association.201729255769
is it possible to develop cancer vaccines to neoantigens, what are the major challenges, and how can these be overcome? neoantigens: nothing new in spite of the name.the term "neoantigen," as applied to molecules newly expressed on tumor cells, has a long history. the groundbreaking discovery of a cancer causing virus in chickens by rous over 100 years ago, followed by discoveries of other tumor-causing viruses in animals, suggested a viral etiology of human cancers. the search for other oncogenic viruses in the 1960s and 1970s resulted in the discoveries of epstein-barr virus (ebv), hepatitis b virus (hbv), and human papilloma virus (hpv), and continues unt ...201729254980
two girl patients with medulloblastoma. case childhood, the most common type of brain tumors is medulloblastoma, a highly malignant primary brain tumor that is found in the cerebellum or posterior fossa. the tumor mass increases and generates obstructive hydrocephalus. risk factors (that might be involved in some cases) include the genetic syndrome such as type 1 neurofibromatosis, exposure to ionizing radiation and epstein-barr virus. medulloblastoma is associated with recessively inherited turcot disease and with conditions as ataxia- ...201729250697
lymphoproliferative disorder in a twin female teenager post kidney lymphoproliferative disorder (ptld) is defined as a heterogeneous group of lymphoid and plasmocytic proliferations with variable malignant potential. they often arise in immunocompromised post solid organ transplant (sot) patients linked with epstein-barr virus (ebv) infection. clinical manifestations include fever, lymphadenopathy and organ involvement. diagnosis of ptld requires morphopathological tissue examination. treatment of ebv-related ptld in sot patients includes immuno ...201729250687
plasma magnesium is inversely associated with epstein-barr virus load in peripheral blood and burkitt lymphoma in uganda.epstein-barr virus (ebv) causes endemic burkitt lymphoma (ebl). ebv control was improved by magnesium (mg2+) supplementation in xmen, an x-linked genetic disease associated with mg2+ deficiency, high circulating ebv levels (viral loads), and ebv-related lymphomas. we, therefore, investigated the relationship between mg2+ levels and ebv levels and ebl in uganda.201729248801
modulation of the tumor microenvironment by epstein-barr virus latent membrane protein 1 in nasopharyngeal carcinoma.latent membrane protein 1 (lmp1) is a primary oncogene encoded by the epstein-barr virus, and various portions of lmp1 are detected in nasopharyngeal carcinoma (npc) tumor cells. lmp1 has been extensively studied since the discovery of its transforming property in 1985. lmp1 promotes cancer cell growth during npc development and facilitates the interaction of cancer cells with surrounding stromal cells for invasion, angiogenesis, and immune modulation. lmp1 is detected in 100% of pre-invasive np ...201729247573
ebi2 receptor regulates myelin development and inhibits lpc-induced demyelination.the g protein-coupled receptor ebi2 (epstein-barr virus-induced gene 2) is activated by 7α, 25-dihydroxycholesterol (7α25hc) and plays a role in t cell-dependant antibody response and b cell migration. abnormal ebi2 signaling is implicated in a range of autoimmune disorders; however, its role in the cns remains poorly understood.201729246262
expression and clinical value of programmed cell death-ligand 1 (pd-l1) in diffuse large b cell lymphoma: a retrospective study.the programmed cell death-1 (pd-1)/programmed cell death-ligand 1 (pd-l1) pathway inhibits the activation of t cells and plays a crucial role in the negative regulation of cellular and humoral immune responses. diffuse large b-cell lymphoma (dlbcl) is the most common lymphoid malignancy in adults. in the present study, we aimed to detect the expression of pd-l1 in dlbcl and to analyze its relationship with prognosis.201729246182
an inflammatory myofibroblastic tumor in the transplanted liver displaying quick wash-in and wash-out on contrast-enhanced ultrasound: a case report.inflammatory myofibroblastic tumor (imt) is an uncommon mesenchymal neoplasm, and its presence in a grafted liver is exceedingly rare.201729245287
an extremely rare case of epstein-barr virus-associated gastric carcinoma with differentiation to neuroendocrine carcinoma.epstein-barr virus (ebv)-associated gastric carcinoma (ebvgc) is defined as a neoplasm comprising monoclonal proliferation of ebv-infected gastric epithelial cells. although the typical histology is gastric carcinoma with lymphoid stroma (gcls), the histologic features of the tumor vary. we report herein the case of a 78-year-old man with multiple simultaneous ebvgcs revealing different histopathologic morphologies; one was mixed adenoneuroendocrine carcinoma (manec), and the other was gcls. bot ...201829244230
symptomatic cerebrospinal fluid hiv-1 escape with no resistance-associated mutations following low-level plasma viremia.the majority of neurologically symptomatic cerebrospinal fluid hiv-1 escape cases are connected with resistance-associated mutations and potentially explained by low cerebrospinal fluid antiretroviral concentrations. however, there are still significant knowledge gaps regarding the physiopathology and long-term management of neurosymptomatic viral escape. we report a case of parkinson-like syndrome following cerebrospinal fluid hiv-1 escape in a 40-year-old female patient with an history of pers ...201729243133
clinical, viral and genetic characteristics of drug reaction with eosinophilia and systemic symptoms (dress) in shanghai, china.dress is one of the most severe drug reactions. the aim of this retrospective study was to summarize the clinical presentation, genetic predisposition and prognostic factors of dress. a total of 52 patients with dress, who were inpatients at a medical referral centre in shanghai, china, from january 2011 to december 2016, were analysed retrospectively. all the patients had skin eruption, 83% had liver involvement, and ≤10% had other organ involvement. average cost of hospitalization was $5,511±3 ...201729242946
a distinct subtype of epstein barr virus positive t/nk-cell lymphoproliferative disorder: adult patients with chronic active epstein barr virus infection-like features.the characteristics of adult patients with chronic active epstein-barr virus infection (adult-onset caebv) are poorly recognized, hindering early diagnosis and an improved prognosis. adult-onset caebv (n = 54) diagnosed between 2005 and 2015 were conducted. adult-onset was defined as an estimated age of onset ≥15 years. to characterize the clinical features of adult-onset caebv, we compared them to those of pediatric-onset (estimated age of onset <15 years) patients (n = 75). we compared the pro ...201729242302
early experience with clinimacs prodigy ccs (ifn-gamma) system in selection of virus-specific t cells from third-party donors for pediatric patients with severe viral infections after hematopoietic stem cell transplantation.viral reactivation is a frequent complication of allogeneic hematopoietic stem cell transplantation especially in children. for refractory cases, rapid virus-specific t-cell therapy would be ideally implemented within a few days. over the course of a year in our pediatric cohort of 43 allogeneic transplantation, 9 patients fulfilled criteria for virus-specific t-cell therapy. viral infections were due to cytomegalovirus (cmv) in 3, epstein-barr virus (ebv) in 2, and adenovirus (adv) in 1 case, w ...201729239916
lymphoepithelioma is a nonkeratinizing squamous cell carcinoma with epstein-barr virus infection in china.the objective of this study is to investigate the histogenesis of lymphoepithelial carcinoma (lec) and its relationship with epstein-barr virus (ebv).201729237908
pi3kδ activates e2f1 synthesis in response to mrna translation stress.the c-myc oncogene stimulates ribosomal biogenesis and protein synthesis to promote cellular growth. however, the pathway by which cells sense and restore dysfunctional mrna translation and how this is linked to cell proliferation and growth is not known. we here show that mrna translation stress in cis triggered by the gly-ala repeat sequence of epstein-barr virus (ebv)-encoded ebna1, results in pi3kδ-dependent induction of e2f1 mrna translation with the consequent activation of c-myc and cell ...201729235459
prevalence of epstein-barr virus dna and porphyromonas gingivalis in japanese peri-implantitis patients.peri-implantitis (pi) is an inflammatory reaction associated with functional deterioration of supporting bones around the dental implant. recent studies suggested epstein-barr virus (ebv) is involved in the pathogenesis of periodontitis. we investigated the association between ebv and porphyromonas gingivalis in japanese pi patients.201729233156
ebv-encoded mirnas target atm-mediated response in nasopharyngeal carcinoma.nasopharyngeal carcinoma (npc) is a highly invasive epithelial malignancy that is prevalent in southern china and southeast asia. it is consistently associated with latent epstein-barr virus (ebv) infection. in npc, mir-barts, the ebv-encoded mirnas derived from bamh1-a rightward transcripts are abundantly expressed and contribute to cancer development by targeting various cellular and viral genes. in this study, we establish a comprehensive transcriptional profile of ebv-encoded mirnas in a pan ...201729230817
posttransplant lymphoproliferative disorders in kidney transplant recipients: a retrospective cohort analysis over two decades in hong characterize the posttransplant lymphoproliferative disorders (ptld) including the epstein-barr virus (ebv) status, histological subgroups, site of occurrence and the clinical outcome in the chinese kidney transplant recipients.201729228580
epstein-barr virus-positive extranodal marginal zone lymphoma of bronchial-associated lymphoid tissue in the posttransplant setting: an immunodeficiency-related (posttransplant) lymphoproliferative disorder?posttransplant lymphoproliferative disorders (ptlds) are a heterogeneous group of hematolymphoid proliferations arising in the context of chronic immunosuppression. the common and indolent b-cell lymphomas, including extranodal marginal zone lymphomas (enmzls) of mucosa-associated lymphoid tissue (malt), are excluded from the category of ptld in the current world health organization classification.201729228090
n-acetylcysteine (nac) ameliorates epstein-barr virus latent membrane protein 1 induced chronic inflammation.chronic inflammation results when the immune system responds to trauma, injury or infection and the response is not resolved. it can lead to tissue damage and dysfunction and in some cases predispose to cancer. some viruses (including epstein-barr virus (ebv)) can induce inflammation, which may persist even after the infection has been controlled or cleared. the damage caused by inflammation, can itself act to perpetuate the inflammatory response. the latent membrane protein 1 (lmp1) of ebv is a ...201729228057
prevalence and molecular profiling of epstein barr virus (ebv) among healthy blood donors from different nationalities in qatar.the epstein-barr virus (ebv) is the causative agent of infectious mononucleosis. ebv is highly prevalent lymphotropic herpesvirus and has been linked to several malignancies. transmission is generally by oral secretions, but can be through blood transfusions and organ transplantations. this study aimed to determine the seroprevalence, viremia rates, and circulating genotypes of ebv in healthy blood donors in qatar.201729228016
visualizing single-cell secretion dynamics with single-protein sensitivity.cellular secretion of proteins into the extracellular environment is an essential mediator of critical biological mechanisms, including cell-to-cell communication, immunological response, targeted delivery, and differentiation. here, we report a novel methodology that allows for the real-time detection and imaging of single unlabeled proteins that are secreted from individual living cells. this is accomplished via interferometric detection of scattered light (iscat) and is demonstrated with laz3 ...201829227108
epstein barr virus-associated smooth muscle tumour (ebv-smt) of the urinary bladder. 201829226094
epstein-barr virus-specific immune control by innate lymphocytes.epstein-barr virus (ebv) is a potent b cell transforming pathogen in humans. in most persistently ebv-infected individuals, potent cytotoxic lymphocyte responses prevent ebv-associated pathologies. in addition to comprehensive adaptive t cell responses, several innate lymphocyte populations seem to target different stages of ebv infection and are compromised in primary immunodeficiencies that render individuals susceptible to symptomatic ebv infection. in this mini-review, i will highlight the f ...201729225606
reactivation of hemophagocytic lymphohistiocytosis triggered by antithymocyte globulin.a 16-year-old boy with epstein-barr virus-associated hemophagocytic lymphohistiocytosis (hlh) underwent allogeneic hematopoietic stem cell transplantation after conditioning with fludarabine, melphalan, total body irradiation, and rabbit antithymocyte globulin (atg). a severe, persistent infusion reaction occurred after the initial administration of atg. investigations showed a rapid increase in the levels of liver enzymes and ferritin, and the reactivation of hlh was confirmed by marked hemopha ...201729225252
oral methotrexate-related lymphoproliferative disease presenting with severe osteonecrosis of the jaw: a case report and literature review.long-term methotrexate (mtx) treatment can cause mtx-related lymphoproliferative disorder (mtx-lpd). we experienced a case of mtx-lpd that was associated with severe osteonecrosis of the jaw mimicking medication-related osteonecrosis of the jaw. the patient was an 81-year-old woman with rheumatoid arthritis (ra) who was treated with mtx and bisphosphonate. after 7 years, she was referred to our department for the assessment of giant ulcer and exposure of the alveolar bone of the left maxilla. hi ...201729225245
[clinicopathologic analysis of micronodular thymoma with lymphoid stroma].objective: to investigate the clinicopathologic features of micronodular thymoma with lymphoid stroma(mnt). methods: five cases of mnt diagnosed from january 2007 to december 2016 in henan provincial people's hospital were collected.hematoxylin-eosin staining and immunohistochemistry were used to evaluate the histological and immunophenotypic characteristics in 5 mnt cases. epstein-barr virus (ebv) status was detected by in situ hybridization for ebv-encoded small rna (eber). polymerase chain re ...201729224277
posttransplant lymphoproliferative disorders in epstein-barr virus donor positive/recipient negative lung transplant recipients.epstein-barr virus (ebv) donor positive/recipient negative (d+/r-) status is a significant risk factor for posttransplant lymphoproliferative disorder (ptld) in lung transplant. there are, however, no studies that identify the risk factors for ptld in the ebv d+/r- lung transplant population to guide the decision to proceed with an ebv-positive donor.201829223419
rank expression in ebv positive nasopharyngeal carcinoma metastasis: a ready-to-treat target?epstein barr virus (ebv) related nasopharyngeal carcinoma (npc), is an highly chemo- and radiosensitive endemic malignancy in southeast asia. more than one third of locally advanced cases relapse after curative treatment, especially because of bone, liver and lung metastases. lymphocyte sub-populations favour ebv-associated carcinogenesis and tumour progression and several strategies aim to reverse this phenomenon. receptor activator of nf-kb (rank) and its ligand (rankl), key regulator of bone ...201729221197
epstein-barr virus in tumor-infiltrating b cells of myasthenia gravis thymoma: an innocent bystander or an autoimmunity mediator?the thymus plays a key role in myasthenia gravis (mg), a b cell-mediated autoimmune disorder affecting neuromuscular junction. most mg patients have thymic abnormalities, including hyperplasia and thymoma, a neoplasm of thymic epithelial cells. epstein-barr virus (ebv) is associated with autoimmune diseases and tumors. recently, we showed ebv persistence and reactivation in hyperplastic mg thymuses, suggesting that ebv might contribute to intra-thymic b cell dysregulation in mg patients. here, w ...201729221139
systemic epstein-barr virus-positive t-cell lymphoproliferative disorders of childhood with fulminant leukocytosis and tumor lysis: a case report with autopsy findings.systemic epstein-barr virus (ebv)-positive t-cell lymphoproliferative disorders (t-lpd) of childhood is an extremely rare disease characterized by an aggressive clinical course and very poor prognosis. we report an adolescent male with systemic ebv-positive t-lpd of childhood after primary ebv infection, resulting in a fatal clinical course within 9 days, along with autopsy findings. a 19-year-old male without an immunocompromised status presented with an acute onset of high fever, and was hospi ...201729218495
authors reply to letter to the editor- in response to: "smit d, meyer d, maritz j, et al. polymerase chain reaction and goldmann-witmer coefficient to examine the role of epstein-barr virus in uveitis". 201729215921
direct substrate identification with an analog sensitive (as) viral cyclin-dependent kinase (v-cdk).viral cyclin-dependent kinases (v-cdks) functionally emulate their cellular cdk counterparts. such viral mimicry is an established phenomenon that we extend here through chemical genetics. kinases contain gatekeeper residues that limit the size of molecules that can be accommodated within the enzyme active site. mutating gatekeeper residues to smaller amino acids allows larger molecules access to the active site. such mutants can utilize bio-orthoganol atps for phosphate transfer and are inhibit ...201829215867
epstein-barr virus-associated hemophagocytic lymphohistiocytosis mimicking lymphoma on fdg pet/ct.a 15-year-old boy with fever, pancytopenia, and hepatosplenomegaly was diagnosed as hemophagocytic lymphohistiocytosis (hlh). f-fdg pet/ct showed hypermetabolic foci in the liver, spleen, and bone marrow, as well as multiple fdg-avid lymph nodes, which were highly suggestive of lymphoma. specimens from splenectomy depicted a large number of macrophages/histiocytes with hemophagocytosis of erythrocytes, without evidence of malignancy. considering increased epstein-barr virus (ebv) dna in peripher ...201829215415
bone marrow involvement of epstein-barr virus-positive large b-cell lymphoma in a patient with angioimmunoblastic t-cell lymphoma. 201829214764
the hedgehog signaling pathway emerges as a pathogenic target.the hedgehog (hh) signaling pathway plays an essential role in the growth, development, and homeostatis of many tissues in vertebrates and invertebrates. much of what is known about hh signaling is in the context of embryonic development and tumor formation. however, a growing body of evidence is emerging indicating that hh signaling is also involved in postnatal processes such as tissue repair and adult immune responses. to that extent, hh signaling has also been shown to be a target for some p ...201729214147
strong viral associations with sle among filipinos.epstein-barr virus (ebv) is considered an important environmental factor in sle aetiology, but the relationship between sle and ebv in the filipino population is unknown. we tested associations between sle, lupus-associated autoantibodies and seropositivity for ebv and other herpes viruses in the filipino population.201729214036
characterization of a suppressive cis-acting element in the epstein-barr virus lmp1 promoter.latent membrane protein 1 (lmp1) is a major oncogene encoded by epstein-barr virus (ebv) and is essential for immortalization of b cells by the virus. previous studies suggested that several transcription factors, such as pu.1, rbp-jκ, nfκb, ebf1, ap-2 and stat, are involved in lmp1 induction; however, the means by which the oncogene is negatively regulated remains unclear. here, we introduced short mutations into the proximal lmp1 promoter that includes recognition sites for the e-box and ikaro ...201729213259
tetraspanin cd63 bridges autophagic and endosomal processes to regulate exosomal secretion and intracellular signaling of epstein-barr virus lmp1.the tetraspanin protein cd63 has been recently described as a key factor in extracellular vesicle (ev) production and endosomal cargo sorting. in the context of epstein-barr virus (ebv) infection, cd63 is required for the efficient packaging of the major viral oncoprotein latent membrane protein 1 (lmp1) into exosomes and other ev populations, and acts as a negative regulator of lmp1 intracellular signaling. accumulating evidence has also pointed to intersections of the endosomal and autophagy p ...201729212935
severity of acute infectious mononucleosis correlates with cross-reactive influenza cd8 t-cell receptor repertoires.fifty years after the discovery of epstein-barr virus (ebv), it remains unclear how primary infection with this virus leads to massive cd8 t-cell expansion and acute infectious mononucleosis (aim) in young adults. aim can vary greatly in severity, from a mild transient influenza-like illness to a prolonged severe syndrome. we questioned whether expansion of a unique hla-a2.01-restricted, cross-reactive cd8 t-cell response between influenza virus a-m158 (iav-m1) and ebv bmlf1280 (ebv-bm) could mo ...201729208744
pik3ca mutations are associated with increased tumor aggressiveness and akt activation in gastric cancer.pik3ca mutations are frequent in gastric cancer. however, their pathological and clinical implications are still unclear. we analyzed the clinicopathological characteristics according to the pik3ca mutation status of patients with stage ib-iv disease who underwent gastrectomy between may 2003 and dec. 2005 (cohort 1; n = 302) and of those with stage iv disease who received gastrectomy between jul. 2006 and dec. 2012 (cohort 2; n = 120). pik3ca mutations were detected in 40 patients (13.2%) in co ...201729207615
etiology of sinonasal inverted papilloma: a narrative review.sinonasal inverted papilloma (ip) is a benign and uncommon tumor of the nasal cavity and paranasal sinuses with a tendency for recurrence and even malignant transformation. though the morphology and clinical behavior of this lesion has been well described, its etiology remains controversial.201729204580
lymphoepithelial carcinoma: a case report of a rare tumor of the larynx.lymphoepithelial carcinoma is a tumor mostly diagnosed in the nasopharynx, but it has also been described in a variety of nonnasopharyngeal sites. it is extremely rare in the larynx and should be distinguished from squamous cell carcinoma. therefore, it must be known by clinicians, pathologists and oncologists. in this case report, we discuss its etiopathogeny, its epidemiological, clinical, pathological and therapeutic aspects, and its outcome.201729204101
the role of the early social environment on epstein barr virus infection: a prospective observational design using the millennium cohort study.epstein-barr virus (ebv) is a highly prevalent herpesvirus linked to infectious mononucleosis and several malignancies. this paper aims to study the association between children's early life social environment at 9 months and ebv infection at 3 years of age.201729202893
epstein-barr virus induces adhesion receptor cd226 (dnam-1) expression during primary b-cell transformation into lymphoblastoid cell lines.epstein-barr virus (ebv), an oncogenic herpesvirus, infects and transforms primary b cells into immortal lymphoblastoid cell lines (lcls), providing a model for ebv-mediated tumorigenesis. ebv transformation stimulates robust homotypic aggregation, indicating that ebv induces molecules that mediate cell-cell adhesion. we report that ebv potently induced expression of the adhesion molecule cd226, which is not normally expressed on b cells. we found that early after infection of primary b cells, e ...201729202043
a rare case of chronic active epstein-barr virus (ebv) infection accompanied by the infiltration of ebv-infected cd8+ t cells into the muscle.we describe a rare case of chronic active epstein-barr virus (caebv) infection, with infiltration of the skeletal muscle. a 19-year-old woman with swollen cervical lymph nodes and a fever was referred to our hospital. swelling of the trapezium muscle and elevation of creatinine kinase level were observed. biopsy results of the brachialis muscle revealed infiltration of epstein-barr virus (ebv)-encoded rna-positive cd8 t lymphocytes. the ebv virus load in the peripheral blood was high, and ebv mo ...201729200172
clinical challenges: identification of patients with novel primary immunodeficiency syndromes.novel primary immunodeficiency disorders are being identified with next generation sequencing technologies. we describe 1 patient with cytotoxic t-lymphocyte-associated protein 4 (ctla-4) haploinsufficiency who had recurrent enhancing brain lesions, nodular pulmonary infiltrates, hepatosplenomegaly, immune cytopenias, as well as progressive hypogammaglobulinemia and lymphopenia. we describe a second patient with activated p110δ syndrome (apds)/p110δ activating mutation causing senescent t cells, ...201729200144
peroxiredoxin-2 and zinc-alpha-2-glycoprotein as potentially combined novel salivary biomarkers for early detection of oral squamous cell carcinoma using proteomic effective screening method is available for oral squamous cell carcinoma (oscc) that is recognized to influence by environmental factors as well as human papillomavirus (hpv) and epstein-barr virus (ebv). therefore, we sought to identify salivary biomarkers for screening of oscc with or without hpv and/or ebv infection. saliva, lesion and oral exfoliated cells were collected from oscc patients and cancer-free controls (cfcs) and grouped depending on their hpv- and ebv-infection status. saliva ...201829199150
comparison of abbott architect®, siemens immulite®, and diasorin liaison® for determination of epstein-barr virus serological diagnosis.this study compared the performance of 3 automated immunoassays, architect® (abbott), immulite® (siemens) and liaison® (diasorin), for epstein-barr virus (ebv) serology. ninety-one serum samples collected in amiens university hospital were analyzed for the presence of viral capsid antigen (vca) igg and igm and epstein-barr nuclear antigen (ebna) igg. the agreement between the 3 assays was calculated for each marker individually and for determination of the ebv profile, based on interpretation of ...201829198371
ebv+ hhv-8+ multicentric castleman disease with plasmablastic aggregates in an hiv+ man: an evolving clinicopathologic entity.we report a case of ebv+ and hhv-8+ multicentric castleman disease with plasmablastic aggregates in an hiv-positive individual. a 41-year-old man presented in early 2015 with fevers, sweats, weight loss, intractable itching, and on subsequent testing was found to be hiv positive. investigations showed cervical lymphadenopathy and splenomegaly. he was treated for hiv and his symptoms resolved. his symptoms recurred in january 2016, and a provisional diagnosis of multicentric castleman disease was ...201729198143
potential cancer chemopreventive and anticancer constituents from the fruits of ficus hispida l.f. (moraceae).ficus hispida l.f. (moraceae) has been used as alternative for traditional medicine in the treatment of various ailments including cancer-cure. the aim of this study was to evaluate the cancer chemopreventive and anticancer activities of crude extracts of f. hispida, with the objective to screen the inhibition of epstein-barr virus early antigen, and cytotoxic active components, and provide foundation for potential applications of this promising medical plant.201829197545
methotrexate-associated lymphoproliferative disease with multiple pulmonary nodules in a patient with rheumatoid arthritis.a 62-year-old woman with rheumatoid arthritis and secondary sjögren's syndrome took methotrexate (mtx) 5 mg three times a week regularly but gradually developed an intermittent fever, oral ulcers and productive cough with mucopurulent sputum for about 2 weeks. image study found multiple nodular lesions and lymphadenopathies in bilateral lungs. empirical antibiotics for 1 week failed to alleviate the fever. a transbronchial biopsy in the right fourth bronchus showed infiltration of abnormally enl ...201729196307
characterization of the ebv-induced persistent dna damage response.epstein-barr virus (ebv) is an oncogenic herpesvirus that is ubiquitous in the human population. early after ebv infection in vitro, primary human b cells undergo a transient period of hyper-proliferation, which results in replicative stress and dna damage, activation of the dna damage response (ddr) pathway and, ultimately, senescence. in this study, we investigated ddr-mediated senescence in early arrested ebv-infected b cells and characterized the establishment of persistent dna damage foci. ...201729194355
clinical significance of plasma epstein-barr virus dna in pulmonary lymphoepithelioma-like carcinoma (lelc) patients. 201729191777
pretreatment epstein-barr virus dna in whole blood is a prognostic marker in peripheral t-cell lymphoma.because there are few studies regarding the clinical impact of circulating ebv-dna in peripheral t-cell lymphomas (ptcls), we tried to evaluate the role of ebv-dna in whole blood as a prognostic factor for ptcl. we retrospectively reviewed 110 ptcl patients with median age of 63 (20-94) years. forty-seven patients (42.7%) showed positive results for ebv-dna, and these patients also had stage iii/iv disease, elevated lactic dehydrogenase, and low albumin level (p = 0.007, p = 0.004, p = 0.002, re ...201729190917
in response to: smit d, meyer d, maritz j, et al. "polymerase chain reaction and goldmann-witmer coefficient to examine the role of epstein-barr virus in uveitis". 201729190161
epstein-barr virus clonality and plasmacytosis in a patient with atypical angioimmunoblastic t cell lymphoma. 201729189897
viral dnaemia and immune suppression in pediatric sepsis.demonstrate that dna viremia is common in pediatric sepsis and quantitate its associations with host immune function and secondary infection risk.201829189638
infusions of epstein-barr virus-specific cytotoxic t lymphocytes as post-remission therapy in high-risk post-transplant lymphoproliferative disorder patients: report of two cases.conventional therapeutic approaches to post-transplant lymphoproliferative disorder (ptld) occurring after solid-organ transplantation have shown only limited success in achieving durable response. key factors driving the pathogenesis of ptld include epstein-barr virus (ebv) reactivation and impaired immune surveillance due to prolonged immune suppression. thus, ebv-specific cytotoxic t lymphocytes (ebv-ctls) have emerged as an alternative therapeutic approach for the treatment of ebv-associated ...201729188583
[symmetrical lesions of the cerebral peduncles and internal capsules on mri in a patient with extranodal nk/t cell lymphoma].a 72-year-old man was admitted to our hospital because of subacute progressive consciousness disturbance. flair images showed diffuse high intensity areas in the bilateral thalamus, basal ganglia, and white matter. chest ct revealed a mass in the right lung. the patient was diagnosed with extranodal natural killer/t cell lymphoma (enkl) according to the results of a biopsy of the right pulmonary mass. the flair images showed diffuse white matter lesions, without a mass such as lymphomatosis cere ...201729187687
relationship between ppp1r15a gene polymorphism (rs611251) and epstein-barr virus-associated tumors.protein phosphatase 1, regulatory subunit 15a (ppp1r15a), also known as growth arrest and dna damage-inducible protein gadd34, plays a vital role in promoting cell death and the unfolded protein response (upr). in order to explore whether the snp (rs611251) of ppp1r15a gene has a role in different types of epstein-barr virus (ebv) - associated tumors, we detected the ppp1r15a gene rs611251 polymorphism in 195 cases of ebv positive tumors (93 lymphomas, 48 gastric carcinomas, 54 nasopharyngeal ca ...201729186961
analysis of dna topology of ebv minichromosomes in hek 293 cells.simian virus 40 (sv40) and epstein-barr virus (ebv) are frequently used as model systems to study dna replication. their genomes are both circular duplex dnas organized in a single replicon where replication initiates at a precise site upon binding of a specific protein: the large tumor (t) antigen for sv40 and the epstein-barr nuclear antigen 1 (ebna-1) for ebv. despite the abundant information available on the genetics and biochemistry of the replication process in these systems, little is kno ...201729186176
viral oncology: molecular biology and pathogenesis.oncoviruses are implicated in approximately 12% of all human cancers. a large number of the world's population harbors at least one of these oncoviruses, but only a small proportion of these individuals go on to develop cancer. the interplay between host and viral factors is a complex process that works together to create a microenvironment conducive to oncogenesis. in this review, the molecular biology and oncogenic pathways of established human oncoviruses will be discussed. currently, there a ...201729186062
plasma ebv micrornas in paediatric renal transplant recipients.epstein-barr virus (ebv) was the first human virus identified to express microrna (mirna). to date, 44 mature mirnas are encoded for within the ebv genome. ebv mirnas have not been profiled in paediatric renal transplant recipients. in this study, we investigated circulating ebv mirna profiles as novel biomarkers in paediatric renal transplant patients.201729185211
a rare case of esophageal lymphoepithelial carcinoma without epstein-barr virus infection. 201729183763
[large b-cell lymphoma coexisting with castleman's disease. report of one case].we report a 73-year-old female patient with castleman's disease coexistent with large b cell type non-hodgkin's lymphoma in a right axillary lymphadenopathy. an excisional biopsy was performed: microscopically, the lymph node revealed the presence of numerous plasma cells and small lymphoid cells characteristic of castleman's disease. an analysis of another portion of the specimen revealed lymphoid cells with large abnormal nuclei gathered locally that were cdd 79+, cd 38+ and mum-1+ as well as ...201729182205
development of lymphoma from the donor of haploidentical stem cell transplantation: a case lymphoproliferative disease (ptld) is a serious complication following hematopoietic stem cell transplantation (hsct). the majority of the cases develop during the first year after the transplantation and are associated with reactivation of the epstein-barr virus (ebv); the ebv-induced lymphoproliferation usually includes donor-derived b cells. we herein describe the case of a 28-year-old female patient who developed ebv-negative ptld, namely diffuse large b-cell lymphoma (d ...201729181178
comprehensive review of post-organ transplant hematologic cancers.a higher risk for a variety of cancers is among the major complications of posttransplantation immunosuppression. in this part of a continuing series on cancers posttransplantation, this review focuses on the hematologic cancers after solid organ transplantation. posttransplantation lymphoproliferative disorders (ptlds), which comprise the great majority of hematologic cancers, represent a spectrum of conditions that include, but are not limited to, the hodgkin and non-hodgkin lymphomas. the onc ...201729178667
selective t-cell depletion targeting cd45ra reduces viremia and enhances early t-cell recovery compared with cd3-targeted t-cell depletion.t-cell depletion (tcd) effectively reduces severe graft-versus-host disease in recipients of hla-mismatched allografts. however, tcd is associated with delayed immune recovery and increased infections. we hypothesized that specific depletion of cd45ra+ naive t cells, rather than broad depletion of cd3+ t cells, can preserve memory-immunity in the allografts and confer protection against important viral infections in the early post-transplant period.201729178554
no indication of increased infection rates using low-dose alemtuzumab instead of anti-thymocyte globulin as graft-versus-host disease prophylaxis before allogeneic stem cell transplantation.alemtuzumab as part of the conditioning protocol is effective in reducing graft-versus-host disease (gvhd), but may be associated with increased infection rates, especially when using high doses (ie, 100 mg).201729178248
atypical epstein-barr virus-associated hemophagocytic lymphohistiocytosis simulating lymphadenitis on 18f-fdg pet/ct and its differential diagnosis.diagnosis of epstein-barr virus-associated hemophagocytic lymphohistiocytosis (ebv-hlh) is challenging as its clinical presentation is atypical. here we present a case of atypical ebv-hlh simulating lymphadenitis on fluorrine-18-fluorodeoxyglucose positron emission tomography/computed tomography (18f-fdg pet/ct), with a view to consider this kind of ebv-hlh as a possible differential diagnosis in lymphadenitis. a 68 years old male who had episodic fever accompanied by weight loss and weakness fo ...201729177266
epstein-barr virus (ebv) associated reactive and indeterminate lymphoid proliferations in the lymph node. 201729174725
outcomes among us patients with diffuse large b cell lymphoma are independent of tumor epstein barr virus positivity or immunosuppression.the prevalence, presenting clinical and pathologic characteristics, and outcomes for patients with diffuse large b-cell lymphoma that is epstein-barr virus positive remains uncertain as does the impact of congenital or iatrogenic immunosuppression. patients with newly diagnosed diffuse large b-cell lymphoma with available tissue arrays were identified from the university of iowa/mayo clinic molecular epidemiology resource. patients with human immunodeficiency virus or prior organ transplant were ...201729170255
rapamycin enhances lytic replication of epstein-barr virus in gastric carcinoma cells by increasing the transcriptional activities of immediate-early lytic promoters.epstein-barr virus (ebv), a human herpesvirus, is linked to both epithelial and lymphoid malignancies. induction of ebv reactivation is a potential therapeutic strategy for ebv-associated tumors. in this study, we assessed the effects of rapamycin on ebv reactivation in gastric carcinoma cells. we found that rapamycin upregulated expression of ebv lytic proteins and increased the viral proliferation triggered by the ebv lytic inducer sodium butyrate. reverse transcription-qpcr, luciferase activi ...201729169830
[how to manage ebv reactivation and ebv-ptld, cmv and human herpesvirus 6 reactivation and infection after allogeneic stem cell transplantation: a report of the sfgm-tc (update)].the french society of bone marrow transplantation and cell therapy (sfgm-tc) organizes annually workshops in the attempt to harmonize clinical practices between different francophone transplantation center. here, we report our recommendations regarding the management of epstein barr virus reactivation and lymphoproliferative disorders, cytomegalovirus (cmv) and human herpes virus 6 (hhv6) after allogeneic stem cell transplantation.201729169653
acute cerebellitis in adults: a case report and review of the literature.acute cerebellitis is a rare disease with the majority of cases described in children. little is known about the clinical characteristics and outcome in adults.201729166939
reply to genomic profiles of nasopharyngeal carcinoma: the importance of histological subtyping and epstein-barr virus in situ assays. 201829165787
genomic profiles of nasopharyngeal carcinoma: the importance of histological subtyping and epstein-barr virus in situ assays. 201829165785
human herpesvirus 8 in perinatally hiv-infected children with interstitial lung disease.human herpesvirus 8 (hhv-8) is associated with the pathogenesis of kaposi sarcoma and interstitial pneumonitis in adults. this study aims to evaluate association between hhv-8 and interstitial lung disease in hiv-infected children.201729165615
a case of hodgkin lymphoma mimicking lymphomatoid granulomatosis diagnosed at autopsy.hodgkin lymphoma and lymphomatoid granulomatosis (lyg) are entities that contain a small number of large, variably epstein-barr virus (ebv)-positive neoplastic cells scattered within background non-neoplastic mixed inflammatory infiltrate. the 2 entities can typically be distinguished histologically by the angiocentric and angiodestructive pattern of lymphomatoid granulomatosis (lyg); also, they differ in overall prognosis. herein, we report a case of hodgkin lymphoma in a 64-year-old caucasian ...201729165611
clinicopathological and immunohistochemical study of head and neck langerhans cell histiocytosis from latin america.langerhans cell histiocytosis (lch) is an inflammatory myeloid neoplastic proliferation with variable clinical behavior caused by the accumulation of cd1a+/cd207+ histiocytes, associated with a variable number of eosinophils, lymphocytes, plasma cells and multinucleated giant cells, most commonly observed in male children. lch is uncommon in the head and neck region, occurring as ulcerated and reddened plaques or nodules that cause destruction of adjacent soft tissues and bone. the exact etiolog ...201729164473
tick-borne transmission of murine gammaherpesvirus 68.herpesviruses are a large group of dna viruses infecting mainly vertebrates. murine gammaherpesvirus 68 (mhv68) is often used as a model in studies of the pathogenesis of clinically important human gammaherpesviruses such as epstein-barr virus and kaposi's sarcoma-associated herpesvirus. this rodent virus appears to be geographically widespread; however, its natural transmission cycle is unknown. following detection of mhv68 in field-collected ticks, including isolation of the virus from tick sa ...201729164067
cd56-negative aggressive nk cell leukemia relapsing as multiple cranial nerve palsies: case report and literature review.aggressive natural killer cell leukemia (ankl) is extremely rare and habitually manifests as a systemic disease with multiorgan failure that rapidly evolves to death. the neoplastic natural killer (nk) cells usually harbor the epstein-barr virus (ebv) with a latent viral infection pattern type ii; they often have a cytoplasmic cd3ε+ and surface cd3-, cd2+, and cd56+ immunophenotype, and they show complex genetic abnormalities affecting multiple tumor suppressor genes and oncogenes. we present a ...201729163992
impact of chronic viral infection on t-cell dependent humoral immune response.during the last decades, considerable efforts have been done to decipher mechanisms supported by microorganisms or viruses involved in the development, differentiation, and function of immune cells. pathogens and their associated secretome as well as the continuous inflammation observed in chronic infection are shaping both innate and adaptive immunity. secondary lymphoid organs are functional structures ensuring the mounting of adaptive immune response against microorganisms and viruses. inside ...201729163507
therapeutic strategies against epstein-barr virus-associated cancers using proteasome inhibitors.epstein-barr virus (ebv) is closely associated with several lymphomas (endemic burkitt lymphoma, hodgkin lymphoma and nasal nk/t-cell lymphoma) and epithelial cancers (nasopharyngeal carcinoma and gastric carcinoma). to maintain its persistence in the host cells, the virus manipulates the ubiquitin-proteasome system to regulate viral lytic reactivation, modify cell cycle checkpoints, prevent apoptosis and evade immune surveillance. in this review, we aim to provide an overview of the mechanisms ...201729160853
[infection by the epstein-barr virus between the years 2006-2015 in the health area of santiago de compostela. relationship with age and sex].in spain, the age and sex to which the primary infection by ebv is produced is poorly studied. the objective of this work is to know its relation with the presence of the primary infection by ebv between the years 2006 and 2015 in our health area.201729160648
retraction: the epstein-barr virus-encoded mir-bart22 targets map3k5 to promote host cell proliferative and invasive abilities in nasopharyngeal carcinoma.[this retracts the article doi: 10.7150/jca.15753.].201729158784
immune activation and benefit from avelumab in ebv-positive gastric cancer.response to immune checkpoint therapy can be associated with a high mutation burden, but other mechanisms are also likely to be important. we identified a patient with metastatic gastric cancer with meaningful clinical benefit from treatment with the anti-programmed death-ligand 1 (pd-l1) antibody avelumab. this tumor showed no evidence of high mutation burden or mismatch repair defect but was strongly positive for presence of epstein-barr virus (ebv) encoded rna. analysis of the cancer genome a ...201729155997
conformational dynamics and free energy of bhrf1 binding to bim bh3.the interaction between the bim bh3 peptide and the viral protein bhrf1 is pivotal to understanding the fundamental molecular details of the mechanism used by the epstein-barr virus to trick the mammalian immune system. here, we study the mechanism of binding/unbinding and compute the free energy for the association of the bim peptide to the bhrf1 protein. key elements of the binding mechanism are the conformational rearrangement together with a main free energy barrier of 11.5kcal/mol. the simu ...201829153883
[atypical profile problem in serological diagnosis of ebv].epstein-barr virus (ebv) is the causative agent of infectious mononucleosis. burkitt's lymphoma, nasopharyngeal carcinoma and post-transplant lymphoproliferative diseases are also associated with ebv. diagnosis is frequently based on detection of specific antibodies. using three parameters (anti vca-igm, anti vca-igg and anti ebna-1 igg), it is possible to define infection status and diagnose acute or past infection. however, sometimes the detection of atypical serological profiles makes it diff ...201729153068
the alice-in-wonderland 1955, english psychiatrist john todd defined the alice-in-wonderland syndrome (aiws) as self-experienced paroxysmal body-image illusions involving distortions of the size, mass, or shape of the patient's own body or its position in space, often accompanied by depersonalization and/or derealization. aiws had been described by american neurologist caro lippman in 1952, but todd's report was the most influential. todd named the syndrome for the perceptual disorder of altered body image experienc ...201829151098
host immune response index in gastric cancer identified by comprehensive analyses of tumor immunity.tumor infiltrating lymphocytes (til) in epstein-barr virus (ebv)-associated/microsatellite-unstable (msi) gastric carcinomas (gc) constitute immune-active principal cellular components of tumor microenvironment and contribute to better prognosis. with the remarkable success of cancer immunotherapies, there is an urgent need for a comprehensive understanding of tumor-immune interactions in patients with gc in the context of host immune response. to identify gc subtype-specific immune response gen ...201729147610
spontaneous development of epstein-barr virus associated human lymphomas in a prostate cancer xenograft program.prostate cancer research is hampered by the lack of in vivo preclinical models that accurately reflect patient tumour biology and the clinical heterogeneity of human prostate cancer. to overcome these limitations we propagated and characterised a new collection of patient-derived prostate cancer xenografts. tumour fragments from 147 unsupervised, surgical prostate samples were implanted subcutaneously into immunodeficient rag2-/-γc-/- mice within 24 hours of surgery. histologic and molecular cha ...201729145505
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