fluorescence in situ hybridization is superior for monitoring epstein barr viral load in infectious mononucleosis patients.epstein barr virus (ebv) plays a causal role in some diseases, including infectious mononucleosis, lymphoproliferative diseases and nasopharyngeal carcinoma. detection of ebv infection has been shown to be a useful tool for diagnosing ebv-related diseases. in the present study, we compared the performance of molecular tests, including fluorescence in situ hybridization (fish) and ebv real-time pcr, to those of serological assays for the detection of ebv infection.201728468603
multiple viral infections in primary effusion lymphoma: a model of viral cooperation in lymphomagenesis.primary effusion lymphoma (pel) is a rare b-cell lymphoid neoplasm mainly associated with hiv infection, presenting as pleural, peritoneal, and pericardial effusions. a defining property of pel is its consistent association with kaposi sarcoma associated herpesvirus (kshv) infection, and, in most cases, epstein barr virus (ebv) co-infection. on these grounds, a review of the literature related to viral cooperation and lymphomagenesis can help to understand the complex interplay between kshv and ...201728468596
comprehensive transcriptome and mutational profiling of endemic burkitt lymphoma reveals ebv type-specific differences.endemic burkitt lymphoma (ebl) is the most common pediatric cancer in malaria-endemic equatorial africa and nearly always contains epstein-barr virus (ebv), unlike sporadic burkitt lymphoma (sbl) that occurs with a lower incidence in developed countries. given these differences and the variable clinical presentation and outcomes, we sought to further understand pathogenesis by investigating transcriptomes using rna sequencing (rnaseq) from multiple primary ebl tumors compared with sbl tumors. wi ...201728465297
plasma viral mirnas indicate a high prevalence of occult viral infections.prevalence of kaposi sarcoma-associated herpesvirus (kshv/hhv-8) varies greatly in different populations. we hypothesized that the actual prevalence of kshv/hhv8 infection in humans is underestimated by the currently available serological tests. we analyzed four independent patient cohorts with post-surgical or post-chemotherapy sepsis, chronic lymphocytic leukemia and post-surgical patients with abdominal surgical interventions. levels of specific kshv-encoded mirnas were measured by reverse tr ...201728465156
epstein-barr virus infection and breast invasive ductal carcinoma in egyptian women: a single center experience.a controversy of the role of epstein-barr virus (ebv) infection in breast carcinomas has been reported in the literature.201728462850
gastric cancer in the era of precision medicine.gastric cancer (gc) remains the third most common cause of cancer death worldwide, with limited therapeutic strategies available. with the advent of next-generation sequencing and new preclinical model technologies, our understanding of its pathogenesis and molecular alterations continues to be revolutionized. recently, the genomic landscape of gc has been delineated. molecular characterization and novel therapeutic targets of each molecular subtype have been identified. at the same time, patien ...201728462377
bilateral chorioretinal scars in a child - case report.the eye manifestations of intrauterine infections are multiple, but chorioretinal scars and/or active chorioretinitis are the most frequently reported. when associated with other systemic manifestations of the infection, the diagnosis becomes more obvious, but when eye involvement is the only abnormality, etiology often remains uncertain. we are presenting the case of an 8-year-old female patient whose fundus lesions revealed an unusual choroidoretinopathy, associated with cataract in one eye. h ...201628461838
the multiple sclerosis (ms) genetic risk factors indicate both acquired and innate immune cell subsets contribute to ms pathogenesis and identify novel therapeutic opportunities.multiple sclerosis (ms) is known to be a partially heritable autoimmune disease. the risk of developing ms increases from typically 1 in 1,000 in the normal population to 1 in 4 or so for identical twins where one twin is affected. much of this heritability is now explained and is due almost entirely to genes affecting the immune response. the largest and first identified genetic risk factor is an allele from the mhc class ii hla-drb1 gene, hla-drb1*15:01, which increases risk about threefold. t ...201728458668
development of a robust, higher throughput green fluorescent protein (gfp)-based epstein-barr virus (ebv) micro-neutralization assay.the goal of most prophylactic vaccines is to elicit robust and effective neutralizing antibodies against the human pathogen target. the titer of neutralizing antibodies to epstein-barr virus (ebv) is a useful biomarker for evaluating ebv vaccines. here, the development and optimization of a 96-well micro-neutralization fluorescent imaging assay (fia) using an ebv virus-encoding green fluorescent protein (gfp) to infect adherent ebv recipient cells is reported. the conditions were optimized for g ...201728457783
intravenous immunoglobulin for pediatric neuropsychiatric lupus triggered by epstein-barr virus cerebral infection. 201628457083
"the girl who grew horns": temporal swelling as an atypical presenting symptom of epstein-barr virus infection. 201628457082
increased risk of ptld in lung transplant recipients with cystic lymphoproliferative disease (ptld) is an important cause of morbidity and mortality following lung transplantation. recipients with cystic fibrosis (cf) may have an increased risk of ptld although the literature is limited to single center cohorts. our primary aim is to examine ptld in an adult lung transplant population by utilizing the international society for heart and lung transplantation registry.201728456611
suppression of epstein-barr virus dna load in latently infected nasopharyngeal carcinoma cells by crispr/cas9.epstein-barr virus (ebv) infects more than 90% of the world's adult population. once established, latent infection of nasopharyngeal epithelial cells with ebv is difficult to eradicate and might lead to the development of nasopharyngeal carcinoma (npc) in a small subset of individuals. in this study we explored the anti-ebv potential of crispr/cas9 targeting of ebv genome in infected npc cells. we designed grnas to target different regions of the ebv genome and transfected them into c666-1 cells ...201728456574
parp1 restricts epstein barr virus lytic reactivation by binding the bzlf1 promoter.the epstein barr virus (ebv) genome persists in infected host cells as a chromatinized episome and is subject to chromatin-mediated regulation. binding of the host insulator protein ctcf to the ebv genome has an established role in maintaining viral latency type, and in other herpesviruses, loss of ctcf binding at specific regions correlates with viral reactivation. here, we demonstrate that binding of parp1, an important cofactor of ctcf, at the bzlf1 lytic switch promoter restricts ebv reactiv ...201728456021
primary lymphocyte infection models for kshv and its putative tumorigenesis mechanisms in b cell lymphomas.kaposi's sarcoma-associated herpesvirus (kshv) is the latest addition to the human herpesvirus family. unlike alpha- and beta-herpesvirus subfamily members, gamma-herpesviruses, including epstein-barr virus (ebv) and kshv, cause various tumors in humans. kshv primarily infects endothelial and b cells in vivo, and is associated with at least three malignancies: kaposi's sarcoma and two b cell lymphomas, respectively. although kshv readily infects endothelial cells in vitro and thus its pathogenic ...201728455586
filaggrin gene polymorphism associated with epstein-barr virus-associated tumors in china.mutations of filaggrin gene (flg) have been identified as the cause of ichthyosis vulgaris, while recently flg mutations were found to be associated with gastric cancer. this study aimed to investigate the association of filaggrin polymorphism with epstein-barr virus-associated tumors in china. a total of 200 patients with three types of tumors and 117 normal control samples were genotyped at three common flg mutation loci (rs3126085, k4671x, r501x) by using sequenom massarray technique. the χ ( ...201728455573
epstein-barr virus-positive mucocutaneous ulcer in crohn's disease. a condition to consider in immunosuppressed ibd patients.epstein-barr virus-positive mucocutaneous ulcer (ebvmcu) is a little known entity that can affect the oropharyngeal mucosa, the gastrointestinal tract and the skin. the main risk factor for the development of this lesion is immunosuppression. because its features are similar to other epstein-barr virus-associated lymphoproliferative disorders, a differential diagnosis can sometimes prove challenging. here, we report the case of a man diagnosed with crohn's disease and treated with azathioprine a ...201728454852
oculomotor nerve palsy as a preceding symptom of adult sporadic burkitt lymphoma: a case report and review of the sporadic burkitt lymphoma (bl) is a rare aggressive neoplasm and represents ~1-5% of all lymphomas diagnosed in adults. the disease exhibits an aggressive clinical manifestation, which frequently involves the central nervous system (cns) in the early stages, and is usually accompanied by abnormalities in cerebrospinal fluid (csf) examinations and/or neuroradiography. the current study describes the rare case of a 29-year-old man who presented with oculomotor nerve palsy without palpated ma ...201728454254
elimination of lmp1-expressing cells from a monolayer of gastric cancer ags cells.epstein-barr virus (ebv) latently infects malignant epithelial cells in approximately 10% of all gastric cancers. latent membrane protein 1 (lmp1), an oncogenic protein, plays an important role in malignant transformation in ebv-associated nasopharyngeal carcinoma and b-cell lymphoma; however, its expression has not been detected in ebv-associated gastric cancer. to address why lmp1 has not been detected in ebv-positive gastric tumors, we focused on the interactions between lmp1-positive and -ne ...201728454117
epstein-barr virus-associated gastric cancer reveals intratumoral heterogeneity of pik3ca mutations.recent whole-genome sequencing identified four molecular subtypes of gastric cancer (gc), of which the subgroup of epstein-barr virus-associated gc (ebvagc) showed a significant enrichment of pik3ca mutations. we here aimed to validate independently the enrichment of pik3ca mutations in ebvagc of a central european gc cohort, to correlate ebv status with clinico-pathological patient characteristics and to test for a major issue of gc, intratumoral heterogeneity.201728453696
cytokine production peculiarities in different forms of epstein-barr virus article presented results of the study of basic levels of regulatory cytokines in patients with various forms of epstein-barr virus (ebv) infection. analysis of the dynamics of cytokine profile in patients with ebv infection revealed the opposite changes studied synthesis of proinflammatory and anti-inflammatory cytokines, which was the basis for the establishment of the four types of immune response. the findings confirm the existence of cytokine imbalance with ebv infection. the established ...201728452728
kidney transplant after hematopoietic cell transplant in pediatrics: infectious and immunosuppressive considerations.pediatric patients requiring kidney transplant after hematopoietic cell transplant receive multiple courses of immunosuppression placing them at risk for infection. to elucidate potential risk factors for infection, we compared the immunosuppressive regimens and infectious complications of pediatric kidney transplant recipients at a single institution who had previously undergone hematopoietic cell transplant from different donors to similar patients reported in the literature. among the initial ...201728452096
treatment of primary central nervous system posttransplant lymphoproliferative disorder in an adult kidney transplant recipient: a case report.posttransplant lymphoproliferative disorder is a serious complication of solid-organ transplant. extranodal involvement is common; however, isolated involvement of the central nervous system is extremely rare and represents a particularly difficult therapeutic challenge with no current consensus on optimal treatment. here, we describe a 70-year-old woman who developed epstein-barr virus-related primary central nervous system lymphoma 19 months after kidney transplant. immunosuppression was reduc ...201728447926
splenic epstein-barr virus-associated inflammatory pseudotumor.splenic inflammatory pseudotumor (ipt) is an uncommon lesion with an inflammatory morphologic aspect that often poses a diagnostic challenge. the etiology of ipt can be infectious, autoimmune, reactive, or neoplastic. splenic epstein-barr virus (ebv)-associated ipts form a subset of splenic ipts in which there is a spindle cell component infected by ebv. the best characterized and most frequent subgroup of splenic ebv-associated ipt is ipt-like follicular dendritic cell tumor. this review also f ...201728447898
investigational drugs for nasopharyngeal carcinoma.nasopharyngeal carcinoma (npc) is endemic to southern china and asia and is etiologically associated with the epstein barr virus (ebv). whole exome and genome sequencing (wes, wgs) studies of npc have reported several actionable therapeutic targets, and that the mutational load of npc maybe comparable to that of squamous head and neck cancer. these unique biological characteristics have been exploited as potential targets and a wide range of investigational drugs are being investigated in clinic ...201728446055
hepatitis due to epstein-barr virus and cytomegalovirus: clinical features and determine the frequency of cytomegalovirus (cmv) and epstein-barr virus (ebv) hepatitis among those with acute cmv and ebv infection in a population based setting and to compare these two types of hepatitis and analyze the outcomes.201728446048
lmp1+slamf1high cells are associated with drug resistance in epstein-barr virus-positive farage epstein-barr virus (ebv) affects the clinical outcome of ebv-positive diffuse large b-cell lymphoma (dlbcl) remains largely unknown. the viral oncogene lmp1 is at the crux of tumorigenesis and cell survival. therefore, we examined the association between lmp1high cells drug resistance. we first assessed slamf1 as a surrogate marker for lmp1high cells. lmp1 and its target gene ccl22 were highly expressed in slamf1high farage cells. these cells survived longer following treatment with a combin ...201728445949
post-transplant malignancy after pediatric kidney transplantation: retrospective analysis of incidence and risk factors in 884 patients receiving transplants between 1963 and 2015 at the university of malignancy (ptm) remains a concern among pediatric kidney transplant (pkt) recipients.201728445794
extranodal natural killer/t-cell lymphoma, nasal type: a rare but critical diagnosis. 201728443316
comparison of circulating tumour cells and circulating cell-free epstein-barr virus dna in patients with nasopharyngeal carcinoma undergoing radiotherapy.quantification of epstein-barr virus (ebv) cell-free dna (cfdna) is commonly used in clinical settings as a circulating biomarker in nasopharyngeal carcinoma (npc), but there has been no comparison with circulating tumour cells (ctcs). our study aims to compare the performance of ctc enumeration against ebv cfdna quantitation through digital pcr (dpcr) and quantitative pcr. 74 plasma samples from 46 npc patients at baseline and one month after radiotherapy with or without concurrent chemotherapy ...201628442757
long-term nitrite inhalant exposure and cancer risk in msm.nitrite inhalants (poppers) are commonly used recreational drugs among msm and were previously associated with elevated rates of high-risk sexual behavior, hiv and human herpesvirus type 8 (hhv-8) seroconversion, and transient immunosuppressive effects in experimental models. whether long-term popper use is associated with cancer risk among msm in the haart era is unclear.201728441176
disruption of direct 3d telomere-trf2 interaction through two molecularly disparate mechanisms is a hallmark of primary hodgkin and reed-sternberg classical hodgkin's lymphoma (chl), specific changes in the 3d telomere organization cause progression from mononuclear hodgkin cells (h) to multinucleated reed-sternberg cells (rs). in a post-germinal center b-cell in vitro model, permanent latent membrane protein 1 (lmp1) expression, as observed in epstein-barr virus (ebv)-associated chl, results in multinuclearity and complex chromosomal aberrations through downregulation of key element of the shelterin complex, the telomere repeat binding ...201728436953
chromosomal instability in gastric cancer biology.gastric cancer (gc) is the fifth most common cancer in the world and accounts for 7% of the total cancer incidence. the prognosis of gc is dismal in western countries due to late diagnosis: approximately 70% of the patients die within 5 years following initial diagnosis. recently, integrative genomic analyses led to the proposal of a molecular classification of gc into four subtypes, i.e.,microsatellite-instable, epstein-barr virus-positive, chromosomal-instable (cin), and genomically stable gcs ...201728431273
clinical utility of circulating cell-free epstein-barr virus dna in patients with gastric cancer.recent comprehensive molecular subtyping of gastric cancer (gc) identified epstein-barr virus (ebv)-positive tumors as a subtype with distinct salient molecular and clinical features. in this study, we aimed to determine the potential utility of circulating cell-free ebv dna as a biomarker for the detection and/or monitoring of therapeutic response in patients with ebv-associated gastric carcinoma (ebvagc). the ebv genes-to-ribonuclease p rna component h1 ratios (ebv ratios) in the gc tumors and ...201728430637
latent membrane protein 1 (lmp1) expression in hodgkin lymphoma and its correlation with clinical and histologic parameters.hodgkin lymphoma is one of the most prevalent lymphoproliferative disorders in pakistan; however, no risk factors for this disease have yet to be established in our population. epstein-barr virus (ebv) is a well-known risk factor for hodgkin lymphoma in endemic regions of the world; however, frequency of its association in our population has not been widely studied. latent membrane protein 1 (lmp1) expression by immunohistochemistry (ihc) is a surrogate marker of ebv in hodgkin lymphoma. therefo ...201728427406
her2-specific chimeric antigen receptor-modified virus-specific t cells for progressive glioblastoma: a phase 1 dose-escalation trial.glioblastoma is an incurable tumor, and the therapeutic options for patients are limited.201728426845
epstein-barr virus ensures b cell survival by uniquely modulating apoptosis at early and late times after infection.latent epstein-barr virus (ebv) infection is causally linked to several human cancers. ebv expresses viral oncogenes that promote cell growth and inhibit the apoptotic response to uncontrolled proliferation. the ebv oncoprotein lmp1 constitutively activates nfκb and is critical for survival of ebv-immortalized b cells. however, during early infection ebv induces rapid b cell proliferation with low levels of lmp1 and little apoptosis. therefore, we sought to define the mechanism of survival in th ...201728425914
epstein-barr virus (ebv)-encoded small rnas (ebers) associated with poor prognosis of head and neck carcinomas.epstein-barr virus (ebv) is the main cause of nasopharyngeal carcinoma (npc), also found in other head and neck carcinomas (hnsccs) where its role remains controversial.201728423694
epstein-barr virus encoded microrna bart7 regulates radiation sensitivity of nasopharyngeal carcinoma.epstein-barr virus (ebv)-associated nasopharyngeal carcinoma (npc) is very sensitive to radiotherapy. to date, the underlying mechanism remains poorly understood. here, we demonstrated that expression of ebv-encoded microrna bart7 (ebv-mir-bart7) increases responsiveness of npc to radiation treatment by targeting gfpt1/tgfβ1 signaling. gfpt1 is the the key rate-limiting enzyme of the hexosamine signaling pathway and governs tgfβ1 production. tgfβ1, a pleotropic cytokine with the potency to trigg ...201728423621
detection of human papillomavirus genotypes and epstein-barr virus in nasopharyngeal carcinomas at the korle-bu teaching hospital, ghana.nasopharyngeal carcinomas (npc) are endemic in far east asia and commonly harbour epstein-barr virus (ebv) which is known to serve as a key oncogenic promoter. human papillomavirus (hpv) is known to contribute to the pathogenesis of npc. however, in ghana these two viruses have not been linked to npc prevalence. this study was designed to determine the hpv genotypes and ebv involved in npc tissue biopsies. a retrospective study design involving 72 formalin-fixed paraffin-embedded tissue (ffpet) ...201728421207
anti-mog antibody-positive adem following infectious mononucleosis due to a primary ebv infection: a case report.anti-myelin oligodendrocyte glycoprotein (mog) antibodies are detected in various demyelinating diseases, such as pediatric acute disseminated encephalomyelitis (adem), recurrent optic neuritis, and aquaporin-4 antibody-seronegative neuromyelitis optica spectrum disorder. we present a patient who developed anti-mog antibody-positive adem following infectious mononucleosis (im) due to epstein-barr virus (ebv) infection.201728420330
mutational landscape of b-cell post-transplant lymphoproliferative is currently unclear whether post-transplant diffuse large b-cell lymphomas (pt-dlbcl) display a similar genomic landscape as dlbcl in immunocompetent patients (ic-dlbcl). we investigated 50 post-transplant lymphoproliferative disorders (ptlds) including 37 pt-dlbcl samples for somatic mutations frequently observed in ic-dlbcl. targeted next generation sequencing (ngs) using the ion torrent platform and a customized panel of 68 genes was performed on genomic dna. non-tumoural tissue was seque ...201728419429
complementary utility of targeted next-generation sequencing and immunohistochemistry panels as a screening platform to select targeted therapy for advanced gastric cancer.we tested the clinical utility of combined profiling of ion torrent pgm based next-generation sequencing (ngs) and immunohistochemistry (ihc) for assignment to molecularly targeted therapies. a consecutive cohort of 93 patients with advanced/metastatic gc who underwent palliative chemotherapy between march and december 2015 were prospectively enrolled. formalin fixed paraffin embedded tumor biopsy specimens were subjected to a 10 gc panels [epstein barr virus encoding rna in-situ hybridization, ...201728418920
the pd-1, pd-l1 expression and cd3+ t cell infiltration in relation to outcome in advanced gastric signet-ring cell carcinoma, representing a potential biomarker for immunotherapy.recent data supports a potentially significant role for immune checkpoint inhibitors in the treatment of gastric cancer. however, there are few data on the clinical implications of immunotherapy markers in gastric signet-ring cell carcinoma (srcc). we evaluated the expression of programmed cell death protein-1 (pd-1), programmed cell death ligand 1(pd-l1), infiltration by cd3+ t cell, microsatellite instability (msi), and epstein-barr virus (ebv), and the relationship of each factor to survival ...201728418918
epstein-barr virus infection induces genome-wide de novo dna methylation in non-neoplastic gastric epithelial cells.epstein-barr virus (ebv)-positive gastric cancer (gc) shows a higher dna methylation epigenotype. ebv infection can causally induce genome-wide aberrant dna methylation, as previously demonstrated by in vitro infection experiments in the low-methylation gc cell line mkn7. however, whether ebv exerts dna methylation remodelling properties in non-neoplastic epithelial cells remains unclear. here we performed post-infection time-series dna methylation analyses using the immortalized normal gastric ...201728418084
high trait shame undermines the protective effects of prevalence knowledge on state shame following hpv/cin diagnosis in women.human papillomavirus (hpv), and the related, cervical intraepithelial neoplasia (cin), are common yet poorly understood physical conditions. the diagnosis of hpv often elicits shame and guilt, which in turn may undermine psychological and physical health. the current study compared shame and guilt responses to diagnosis among two groups: women diagnosed with hpv/cin and women diagnosed with epstein-barr virus (ebv/im). eighty women recently diagnosed with hpv/cin or ebv/im completed measures of ...201728417294
interaction of epstein-barr virus genes with human gastric carcinoma transcriptome.gastric carcinoma (gc) is a leading cause of mortality. 10% of gc cases are related with ebv (epstein-barr virus) infection. the detailed mechanistic roles ebv genes play and especially the interaction between the viral genes and human genes in gc remain unclear. in this study, raw fastq data from 285 gc samples were downloaded from tcga (the cancer genome atlas), including 25 ebv positive (ebv+) gc samples and 260 ebv negative (ebv-) gc samples. rna-seq based expression data were generated for ...201728415594
ebv-encoded micrornas profile evaluation in pediatric liver transplant recipients.epstein-barr virus (ebv) infects 90% of the world population, commonly causing self-limiting infectious mononucleosis or rarely inciting a range of malignancies. ebv micrornas (mirnas) were discovered by sequencing libraries of small rnas generated from several ebv-positive cell lines. little is known about their roles, but their high stability and easy quantification make these molecules potential biomarkers.201728414949
the epstein-barr virus mir-bhrf1-1 targets rnf4 during productive infection to promote the accumulation of sumo conjugates and the release of infectious modification by the small ubiquitin-like modifier (sumo) regulates a variety of cellular functions, and is hijacked by viruses to remodel the host cell during latent and productive infection. here we have monitored the activity of the sumo conjugation machinery in cells productively infected with epstein-barr virus (ebv). we found that sumo2/3 conjugates accumulate during the late phase of the productive virus cycle, and identified several viral proteins as bone fide sumoylati ...201728414785
hepatic inflammatory pseudotumour-like follicular dendritic cell tumor: a case report.hepatic inflammatory pseudotumor (ipt)-like follicular dendritic cell (fdc) tumor is a rare neoplasm. we herein report the case of 19-year-old female patient with an ipt-like fdc tumor and summarize 24 cases of hepatic fdc tumors previously reported in the english literature. the patient presented with complaints of abdominal discomfort, without significant laboratory abnormalities, and underwent surgical removal of a hepatic tumor. the resected tumor was 6 cm in the longest diameter and the tum ...201728413665
vandetanib and adam inhibitors synergistically attenuate the pathological migration of ebv-infected retinal pigment epithelial cells by regulating the vegf-mediated mapk pathway.the extracellular signals induced by vascular endothelial growth factor (vegf) are implicated in choroidal neovascularization (cnv) and thus, are associated with vision-limiting complications in the human retina. vandetanib is an oral anticancer drug that selectively inhibits the activities of vegf receptor and epidermal growth factor receptor tyrosine kinase; however, the effects of vandetanib on vegf in retinal pigment epithelial (rpe) cells have not yet been studied. in the present study, a c ...201728413487
branchial cleft cyst: an unusual site for the cervical metastasis of nasopharyngeal carcinoma.cancers found in the resected branchial cleft cyst are rare clinically but usually impose substantive diagnostic and treatment challenges for clinicians.201728413147
acute kidney injury in symptomatic primary epstein-barr virus infectious mononucleosis: systematic review.textbooks and reviews do not mention the association of symptomatic primary epstein-barr virus infectious mononucleosis with acute kidney injury in subjects without immunodeficiency or autoimmunity.201728410496
clinicopathologic features of early gastric carcinoma with lymphoid stroma and feasibility of endoscopic submucosal dissection.gastric carcinoma with lymphoid stroma (gcls) is a rare disease known to have unique features and a favorable prognosis. this study aimed to determine the clinicopathologic features of early gcls (egcls) and define the feasibility of endoscopic submucosal dissection (esd) for egcls.201728409376
recent advances in understanding epstein-barr virus.epstein-barr virus (ebv) is a common human herpes virus known to infect the majority of the world population. infection with ebv is often asymptomatic but can manifest in a range of pathologies from infectious mononucleosis to severe cancers of epithelial and lymphocytic origin. indeed, in the past decade, ebv has been linked to nearly 10% of all gastric cancers. furthermore, recent advances in high-throughput next-generation sequencing and the development of humanized mice, which effectively mo ...201728408983
human papillomavirus, epstein-barr virus, and methylation status of p16(ink4a) in penile cancer.little is known about penile carcinogenesis. the aim of this study was to evaluate the prevalence of hpv and ebv, and the methylation status of p16(ink4a) in penile cancer samples, and to contribute to the understanding of the mechanisms responsible for penile cancer development. hpv dna was detected in 63.6% of 122 cases, with hpv16 being the most prevalent type. ebv dna was detected in 47.7%, with ebv-1 being the most prevalent type. hpv/ebv co-infections were found in 27.3% of the cases. hype ...201728403538
human and epstein-barr virus mirna profiling as predictive biomarkers for endemic burkitt lymphoma.endemic burkitt lymphoma (ebl) is an aggressive b cell lymphoma and is associated with epstein-barr virus (ebv) and plasmodium falciparum malaria co-infections. central to bl oncogenesis is the over-expression of the myc proto-oncogene which is caused by a translocation of an ig enhancer in approximation to the myc gene. while whole genome/transcriptome sequencing methods have been used to define driver mutations and transcriptional dysregulation, microrna (mirna) dysregulation and differential ...201728400759
oral and cutaneous lymphomas other than mycosis fungoides and sézary syndrome in a mexican cohort: recategorization and evaluation of international geographical disparities.nonmycosis fungoides/sézary syndrome (non-mf/ss) primary cutaneous lymphomas (pcl) are currently categorized under the 2005-world health organization/european organization for research and treatment of cancer (who-eortc) classification for pcl. these differ in behavior from secondary cutaneous lymphomas (scl) and to lymphomas limited to the oral cavity (primary oral lymphomas [pol]) both categorized under the 2016-who classification for lymphoid neoplasms.201728400635
natural killer/t-cell lymphomas in pediatric and adolescent patients.natural killer/t-cell (nk/t-cell) lymphomas are rare in children and adolescents and consist predominantly of nasal-type extranodal nk/t-cell lymphomas. more than half of pediatric/adolescent patients with nk/t-cell lymphomas present with localized nasal/sinus involvement, but the disease may involve many organs. nk/t-cell lymphoma cells are cytotoxic and associated with necrosis and angioinvasion; they express cd56, cd2, cytoplasmic cd3 epsilon, and to a variable degree cd30. the cells contain ...201728398275
m2-polarized tumour-associated macrophages in stroma correlate with poor prognosis and epstein-barr viral infection in nasopharyngeal assess the relationships between cd163 expression, localization of cd163(+) macrophages, clinicopathological features and prognosis of npc.201728395580
[causes and management of pediatric spontaneous tonsillar haemorrhage].objective: to analyze the causes and management plan of pediatric spontaneous tonsillar haemorrhage(sth). methods: according to the criteria of sth difined by griffies, patients with sth from december 2013 to january 2016 were included in this retrospective study. results: a total of 11 patients were reviewed. the etiological diagnosis included 3 pediatric epstein-barr virus associated infectious mononucleosis(ebv-im), 3 suspected pediatric ebv-im to 3 acute suppurative tonsillitis, 1 acute vira ...201728395496
b-cell and classical hodgkin lymphomas associated with immunodeficiency: 2015 sh/eahp workshop report-part 2.the 2015 workshop of the society for hematopathology/european association for haematopathology submitted small and large b-cell lymphomas (bcls), including classical hodgkin lymphoma (chl), in the context of immunodeficiency.201728395108
ebv-positive b-cell proliferations of varied malignant potential: 2015 sh/eahp workshop report-part 1.the 2015 workshop of the society for hematopathology/european association for haematopathology aimed to review b-cell proliferations of varied malignant potential associated with immunodeficiency.201728395107
t- and nk-cell lymphomas and systemic lymphoproliferative disorders and the immunodeficiency setting: 2015 sh/eahp workshop report-part 4.the 2015 workshop of the society for hematopathology/european association for haematopathology aimed to review immunodeficiency-related t- and natural killer (nk)-cell lymphoproliferations.201728395105
hhv8/kshv-positive lymphoproliferative disorders and the spectrum of plasmablastic and plasma cell neoplasms: 2015 sh/eahp workshop report-part 3.the 2015 workshop of the society for hematopathology/european association for haematopathology aimed to review immunodeficiency-related lymphoproliferative disorders with plasmablastic and plasma cell differentiation.201728395104
chronic active epstein-barr virus associated enteritis may develop into a precancerous disease. 201728394808
multivisceral transplantation for abdominal tumors in children: a single center experience and review of the literature.standard management of intra-abdominal pediatric solid tumors requires complete resection. however, tumors with multiple organ and vascular involvement present a unique surgical challenge. we conducted a retrospective chart review of four patients, aged 2-14 years, undergoing mvt for intra-abdominal tumors with significant involvement of the visceral arteries and/or portomesenteric venous system at our institution. indications for mvt included hepatocellular carcinoma, inflammatory myofibroblast ...201728393434
posttransplantation lymphoproliferative disorder after pediatric solid organ transplantation: experiences of 20 years in a single evaluate the clinical spectrum of posttransplantation lymphoproliferative disorder (ptld) after solid organ transplantation (sot) in children.201728392824
posttransplant lymphoproliferative disorder after clinical islet transplantation: report of the first two cases.we report the first two cases of posttransplant lymphoproliferative disorder (ptld) in recipients of islet transplants worldwide. first, a 44-year-old recipient of three islet infusions developed ptld 80 months after his initial transplantation, presenting with abdominal pain and diffuse terminal ileum thickening on imaging. he was treated with surgical excision, reduction of immunosuppression, and rituximab. seven months later, he developed central nervous system ptld, presenting with vertigo a ...201728390107
cytoreductive conditioning intensity predicts clonal diversity in ada-scid retroviral gene therapy patients.retroviral gene therapy has proved efficacious for multiple genetic diseases of the hematopoietic system, but roughly half of clinical gene therapy trial protocols using gammaretroviral vectors have reported leukemias in some of the patients treated. in dramatic contrast, 39 adenosine deaminase-deficient severe combined immunodeficiency (ada-scid) patients have been treated with 4 distinct gammaretroviral vectors without oncogenic consequence. we investigated clonal dynamics and diversity in a c ...201728351939
airway inflammation and symptoms in children following liver and heart describe the upper airway endoscopic findings of children with upper airway symptoms after liver transplantation (lt) or heart transplantation (ht).201728316109
longitudinal viral dynamics in semen during early hiv infection.multiple viruses coinfect the male genital tract, influencing each other’s replication and perhaps affecting human immunodeficiency virus (hiv) pathogenesis and disease progression.201728174909
isolation and characterization of an hiv-1 envelope glycoprotein-specific b-cell from an immortalized human naïve b-cell library.with the recent development of single b-cell cloning techniques, an increasing number of human immunodeficiency virus type 1 (hiv-1)-specific broadly neutralizing antibodies have been isolated since 2009. however, knowledge regarding hiv-1-specific b cells in vivo is limited. in this study, an hiv-1-specific b-cell line was established using healthy pbmc donors by the highly efficient epstein-barr virus transformation method to generate immortalized human naïve b-cell libraries. the enrichment o ...201728073404
frequency of epstein-barr virus in classical hodgkin lymphoma.epstein-barr virus plays an important role in pathogenesis of hodgkin lymphoma. the first patient with epstein-barr positive reed sternberg cells was described in 1985. since then association between epstein-barr virus and hodgkin lymphoma has been shown in many parts of the world and its occurrence shows significant variation from continent to continent and from country to country.201728718560
[morphological diagnosis of viral damage to a kidney transplant].due to the development of organ transplantation and its accompanying immunosuppression, recent years have been marked by the higher incidence of opportunistic viral infections that are of considerable importance among posttransplantation complications. a variety of viral infections, the possibility of their concomitant or subtle course, and the ability to cause life-threatening complications in recipients make relevant the description of existing diagnostic methods. the article summarizes the da ...201628635942
high levels of il-10 and cd4+cd25hi+ treg cells in endemic burkitt's lymphoma patients.the interplay between epstein-barr virus infection, malaria, and endemic burkitt's lymphoma is not well understood. reports show diminished ebv-specific th1 responses in children living in malaria endemic areas and deficiency of ebna1-specific ifn-γ t cell responses in children with endemic burkitt's lymphoma (ebl). this study, therefore, examined some factors involved in the loss of ebna-1-specific t cell responses in ebl.201528536409
nodal epstein-barr virus-positive t-cell/nk-cell lymphoma associated with immunodeficiency: a rare condition looking for recognition.the authors describe a peculiar form of epstein-barr virus (ebv)-associated t-cell lymphoma in an hiv-positive patient presenting an aggressive clinical course. unlike most other ebv-positive t-cell/natural-killer (nk)-cell lymphomas, the disease was characterized by predominant nodal involvement at presentation. t-cell lineage was confirmed by t-cell receptor-rearrangement, and neoplastic cells exhibited strong and diffuse cd56 expression. a marked intravascular component was detected in the sk ...201728573126
a case of infantile systemic lupus erythematosus with severe lupus nephritis and ebv infection.infantile systemic lupus erythematosus (isle) is extremely rare. patients with isle usually become severely unwell and have poor prognosis. epstein-barr virus (ebv) infection has been implicated in the development of sle in both adults and children. recently, we experienced a case of isle with severe lupus nephritis (ln) and ebv infection. a 14-month-old japanese boy was diagnosed with isle according to the american rheumatism association criteria. renal biopsy showed ln classified as internatio ...201328509290
epstein-barr virus infection and central nervous system involvement after orthoptic liver transplant.epstein-barr virus (ebv) infection presenting as encephalitis in seronegative adults in the context of solid organ transplantation is rarely reported. ebv seroconversion illnesses in the adult population after organ transplantation are quite uncommon. this report describes a case of encephalitis due to ebv infection after liver transplantation in an adult patient. the patient was seronegative for ebv pretransplant. she showed persistent viral replication indicated by high levels of ebv dna in th ...201128839616
association of bladder adenocarcinoma and bk virus infection in a pancreatico-renal transplant recipient.viral infection has been related to post-transplantation tumour development, particularly epstein-barr virus, human papillomavirus, hepatitis b and c viruses, and herpes virus 8. recently, bk virus (bkv) has emerged as an important cause of tumour formation in solid organ transplant recipients. bkv oncogenic potential relates to the ability to inactivate the functions of tumour suppression proteins p53 and prb family, and induction of chromosomal aberrations. we report a case of urinary bladder ...201028657060
quantitative analysis of human leucocyte antigen expression during culture of epstein-barr virustransformed cell lines using the dako qifikit.pre-existing donor-specific human leucocyte antigen (hla) antibodies in renal allograft recipients result in hyperacute and accelerated graft failure. these antibodies can be detected in flow cytometric assay systems using hla-characterised epstein-barr virus (ebv)-transformed b-lymphocyte cell lines. confident assay performance is predicated by the expression of hlas on the ebv-transformed b-cell line surface. surface hla expression of three ebv-transformed b-cell lines that had previously been ...200728700889
triterpenoids from ganoderma lucidum inhibit the activation of ebv antigens as telomerase inhibitors.nasopharyngeal carcinoma (npc) is a malignant disease that threatens the health of humans. to find effective agents for the inhibition of epstein-barr virus (ebv) infection, which is associated with npc, a phytochemical investigation of ganoderma lucidum was carried out in the present study. five triterpenoids were identified, including ganoderic acid a (compound 1), ganoderic acid b (compound 2), ganoderol b (compound 3), ganodermanontriol (compound 4), and ganodermanondiol (compound 5), on the ...201728912878
interferon regulatory factor 5 is a potential target of autoimmune response triggered by epstein-barr virus and mycobacterium avium subsp. paratuberculosis in rheumatoid arthritis: investigating a mechanism of molecular mimicry.rheumatoid arthritis (ra) is a chronic disease characterised by a pro-inflammatory cytokines linked erosive joint damage and by humoral and cellular response against a broad range of self-peptides. molecular mimicry between epstein-barr virus (ebv), mycobacterium avium subsp. paratuberculosis (map) and host peptides has long been regarded as an ra pathogenetic mechanism. using bioinformatic analysis we identified high sequence homology among interferon regulatory factor 5 (irf5), ebv antigen bol ...201829352853
the potential of currently unavailable herpes virus vaccines.despite overwhelming experimental work, there are no licensed vaccines against the most frequent alphaherpesviruses, namely herpes simplex virus 1 and 2 (hsv1 and 2) nor against the epstein-barr virus (ebv), a member of the subfamily gammaherpesvirus. areas covered: since the dnas of both hsvs reside in the regional sensory ganglia in a latent state (i.e. as circularized episomal molecules), a corresponding vaccine might be useful for immunotherapy rather than prevention of primary infection. he ...201829313728
are infectious agents involved in the pathogenesis of postpartum psychosis?since postpartum psychosis has been linked to activation of the immune system, it has been hypothesized that infectious agents may be involved in the pathogenesis of this disorder. we therefore investigated whether exposure to pathogens that can infect the central nervous system is increased in patients with postpartum psychosis.201829310062
viral unmasking of cellular 5s rrna pseudogene transcripts induces rig-i-mediated immunity.the sensor rig-i detects double-stranded rna derived from rna viruses. although rig-i is also known to have a role in the antiviral response to dna viruses, physiological rna species recognized by rig-i during infection with a dna virus are largely unknown. using next-generation rna sequencing (rnaseq), we found that host-derived rnas, most prominently 5s ribosomal rna pseudogene 141 (rna5sp141), bound to rig-i during infection with herpes simplex virus 1 (hsv-1). infection with hsv-1 induced re ...201829180807
epidemiological profile of acute viral study the etiology and clinico-epidemiological profile of acute viral encephalitis in children with acute encephalitis syndrome (aes).201729076102
trim29 promotes dna virus infections by inhibiting innate immune response.many double-stranded dna viruses, such as epstein-barr virus, can establish persistent infection, but the underlying virus-host interactions remain poorly understood. here we report that in human airway epithelial cells epstein-barr virus induces trim29, a member of the trim family of proteins, to inhibit innate immune activation. knockdown of trim29 in airway epithelial cells enhances type i interferon production, and in human nasopharyngeal carcinoma cells results in almost complete epstein-ba ...201729038422
herpesviruses in etiopathogenesis of aggressive periodontitis: a meta-analysis based on case-control studies.previous studies have found that herpesviruses are associated with aggressive periodontitis (agp). however, these findings are controversial. this meta-analysis was aimed at clarifying the association between herpesviruses and agp.201729036216
telomere dynamics in immune senescence and exhaustion triggered by chronic viral infection.the progressive loss of immunological memory during aging correlates with a reduced proliferative capacity and shortened telomeres of t cells. growing evidence suggests that this phenotype is recapitulated during chronic viral infection. the antigenic volume imposed by persistent and latent viruses exposes the immune system to unique challenges that lead to host t-cell exhaustion, characterized by impaired t-cell functions. these dysfunctional memory t cells lack telomerase, the protein capable ...201728981470
persistent herpesvirus infections and telomere attrition over 3 years in the whitehall ii cohort.the determinants of telomere attrition, a potential marker of cellular aging, are not well understood. persistent herpesvirus infections including cytomegalovirus (cmv) infection may be particularly important for telomere dynamics via mechanisms such as inflammation, oxidative stress, and their impact on peripheral blood lymphocyte composition. this study examined the association of 4 human herpesviruses (cmv, herpes simplex virus type 1, human herpesvirus type 6, and epstein-barr virus) with ch ...201728931225
[investigation of bacterial and viral etiology in community acquired central nervous system infections with molecular methods].in this multicenter prospective cohort study, it was aimed to evaluate the bacterial and viral etiology in community-acquired central nervous system infections by standart bacteriological culture and multiplex polymerase chain reaction (pcr) methods. patients hospitalized with central nervous system infections between april 2012 and february 2014 were enrolled in the study. demographic and clinical information of the patients were collected prospectively. cerebrospinal fluid (csf) samples of the ...201728929964
pcr detection of human herpesviruses in colonic mucosa of individuals with inflammatory bowel disease: comparison with individuals with immunocompetency and hiv infection.detection of human herpesviruses (hhvs) other than cytomegalovirus (cmv) in colonic mucosa of individuals with inflammatory bowel disease (ibd) remains unknown. this study identified eight hhvs in the colonic mucosa of individuals with ibd and compared the results with immunocompetent and human immunodeficiency virus (hiv)-infected individuals.201728902907
seroprevalence of cytomegalovirus in donors & opportunistic viral infections in liver transplant recipients.opportunistic virus infections are common in liver transplant (lt) recipients. there is a risk of developing infection with cytomegalovirus (cmv) and herpes-related viruses such as herpes simplex virus-1 and 2 (hsv-1 & 2), epstein-barr virus (ebv) and varicella zoster virus (vzv), reactivation of infection and recurrent infection. this study was conducted to determine cmv seropositivity in donors and its influence on lt recipients and seropositivity of cmv, hsv-1 and 2, eb viral capsid antigen ( ...201728862190
low cd4 cells and viral co-infection increase the risk of vain: use of scca1 and ki67 as diagno-prognostic biomarkers.this study evaluated the correlation of scca1, ki67 and cd4 cell expressions and classified vaginal smears in individuals co-infected with human immunodeficiency virus (hiv), herpes simplex virus 2 (hsv2), epstein barr virus (ebv) and human papilloma virus (hpv). this crossectional study included 173 participants within the age range of 20-70 years. vaginal smears were stained by papanicolaou technique and classified into high-grade squamous cell intraepithelial lesion (hsil), low-grade squamous ...201729269193
the role of fungi in the etiology of multiple sclerosis.multiple sclerosis (ms) is a chronic inflammatory disorder of the central nervous system. infectious triggers of ms are being actively investigated. substantial evidence supports the involvement of the epstein-barr virus (ebv), though other viruses, bacteria, protists, and fungi are also being considered. many links between fungi and diseases involving chronic inflammation have been found recently. evidence linking ms and fungi is reviewed here. the hla-drb1*15 allele group is the most important ...201729085329
herpesvirus and autophagy: "all right, everybody be cool, this is a robbery!"autophagy is an essential vacuolar process of the cell, leading to lysosomal degradation and recycling of proteins and organelles, which is extremely important in maintaining homeostasis. multiple roles have been now associated with autophagy, in particular a pro-survival role in nutrient starvation or in stressful environments, a role in life span extension, in development, or in innate and adaptive immunity. this cellular process can also take over microorganisms or viral proteins inside autop ...201729207540
report of a myocarditis outbreak among pediatric patients: human herpesvirus 7 as a causative agent?the etiology of myocarditis in children has not yet been completely elucidated.201729206935
[detection of herpes virus and human enterovirus in pathology samples using low-density arrays].despite the frequency of infections with herpesviridae family, only eight subtypes affect humans (herpex simplex virus types 1 and 2, varicella zoster virus, epstein-barr virus, citomegalovirus and human herpes virus types 6, 7 and 8). amongst enteroviruses infections, the most important are poliovirus, coxackievirus and echovirus. symptoms can vary from mild to severe and early diagnosis is of upmost importance. nowadays, low-density arrays can detect different types of viruses in a single assa ...201729179969
acute viral infections of the central nervous system, 2014-2016, order to investigate the viral etiology of acute infections of central nervous system (cns), multiplex and single pcrs combined with serology for arboviruses were applied on samples from 132 hospitalized patients in greece during may 2014-december 2016. a viral pathogen was detected in 52 of 132 (39.4%) cases with acute cns infection. enteroviruses predominated (15/52, 28.8%), followed by west nile virus (9/52, 17.3%). phleboviruses, varicella-zoster virus, and epstein-barr virus accounted fo ...201729168889
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