[proceedings: transfer of eb virus genome from lymphocytes to epithelial cells]. 1975168135
lymphoproliferative disease in a cotton-top marmoset after inoculation with infectious mononucleosis-derived epstein-barr virus.injection of concentrated ebv derived from cells of the kaplan line of infectious mononucleosis (im) origin resulted in malignant lymphoproliferation in one out of three cotton-top marmosets 6 weeks after inoculation. two additional animals receiving the same isolate after incubation with an antibody-containing human serum did not develop tumors. inoculation of concentrated virus derived from the p3hr-1 line of burkitt origin did not lead to lymphoproliferations in five marmosets. three of these ...1975168158
antibodies to epstein-barr virus-associated nuclear antigen and to other viral and non-viral antigens in hodgkin's disease.antibody reactivity to epstein-barr virus (ebv)-associated nuclear antigen (ebna) was investigated by means of the anticomplement immunofluorescence technique on sera from patients with hodgkin's disease (hd) and from appropriate controls. antibody levels to other ebv-determined antigens, i.e. viral capsid (vca) and early antigens (ea), and to measles and rubella viruses, to cytomegalovirus (cmv), and to toxoplasma gondii were also measured. the results of anti-ebv antibody titrations demonstrat ...1975168161
epidemiological studies of epstein-barr herpesvirus infection in western a study of a caucasian population in western australia the prevalence of antibodies to epstein-barr virus (ebv) was 41% in the 9- to 10-year age group, 80% in the 16 to 19-year age group and 92% in young adults. the age-specific annual seroconversion rates indicated two peaks of primary ebv infection in the population studied - one under 5 years of age and the other at adolescence. the geometric mean titre rose with age, from 23 at 5-6 years to 53 at 36-40 years. it was shown that in 73 famil ...1975168249
enhanced destruction of lymphoid cell lines by peripheral blood leukocytes taken from patients with acute infectious mononucleosis.the cytotoxicity of peripheral blood leukocytes from normal human donors and from patients with ebv-associated infectious nomonucleosis (im) has been determined for human lymphoid cell lines (lcl) containing epstein-barr virus (ebv) dna. in a 51cr release assay, mononuclear leukocytes from all donors are spontaneously cytotoxic. leukocytes taken from patients within the first 2 weeks of overt im are significantly more cytotoxic. this increased cytotoxicity declines to the spontaneous level as th ...1975168255
the importance of geographical pathology.three different forms of cancer--the burkitt lymphoma, nasopharyngeal carcinoma and skin cancer--are discussed in their relation to geographical distribution, the possible role of viruses, and genetic predisposition. certain general conclusions in regard to cancer follow from these discussions. it is stated firmly that cancer is not a disease but a tissue manifestation which has not only many different presentations but also many different complex causes which consequently will need many differe ...1975168477
letter: interpersonal transmission of eb-virus infection. 1975168494
localization and quantitation of ebv-associated nuclear antigen (ebna) in raji cells.epstein-barr virus (ebv)-associated nuclear antigen (ebna) was localized to chromatin fibers of raji cells. when ebna was labeled with the horseradish-peroxidase technique of sternberger a strong reaction covered most fiber surfaces as observed by electron microscopy. the dry mass of raji chromatin increased after exposure to both ebv-positive and -negative serum, but the increase was significantly larger in the material treated with positive serum.1975168861
immortalizing and nonimmortalizing laboratory strains of epstein-barr virus. 1975169031
studies on the epstein-barr virus genome and the ebv-determined nuclear antigen in human malignant disease. 1975169032
ebv dna in nonlymphoid cells of nasopharyngeal carcinomas and in a malignant lymphoma obtained after inoculation of ebv into cottontop marmosets. 1975169033
plasmid dna as a possible state of epstein-barr virus genomes in nonproductive has been shown that the ebv dna in raji cells is located in chromosomes and that most of this ebv dna can be separated from high molecular weight cell dna both in alkaline and neutral glycerol gradients. the data indicate that the latent ebv dna may exist possibly as a plasmid dna. as 50 genomes of ebv dna are equal to 0.1% of the total cell dna, it may be quite conceivable that such an amount of dna should remain in a non-integrated form. chromosomal location may be a requisite for any dna t ...1975169034
sequence of epstein-barr virus productive cycle in human lymphoblastoid cells. 1975169035
antibodies to epstein-barr virus in subacute sclerosing panencephalitis patients.blood samples from 45 cases of subacute sclerosing panencephalitis in israel were tested for the presence of antibodies to epstein-barr virus capsid antigen and early antigen by the indirect immunofluorescence method. significantly higher titers were found in patients as compared to healthy controls with higher titers especially in jews of asian-african origin. mean geometric titer was 1:42.8 in this group as compared to 1:10.8 in the control group. antibodies to epstein-barr virus early antigen ...1975169130
diseases and mechanisms of persistent dna virus infection: latency and cellular transformation. 1975169310
letter: immunodiffusion studies of lymphoblast cell antigens. 1975169368
inhibitory effect of adult bovine serum on release of infectious epstein-barr virus from a virus-carrier cell line.when certain bovine sera (bs) were used to culture p3hr-1 cells, the amount of infectious epstein-barr virus (ebv) released into the culture fluid was significantly less than that obtained when calf serum (cs) or fetal calf serum (fcs) was used. a direct dose-response relationship was noted between the concentration of bs and the degree of inhibition of virus production. cell growth was not inhibited nor was virus infectivity neutralized by bs. similarly, ebv infectivity was not reduced by spent ...1975169373
editorial: leukaemia in animals and man. 1975169460
parallel tubular structures in lymphocytes. ii. correlation with cellular immunity and cytomegalovirus and epstein-barr virus antibodies in hodgkin's disease.the increased incidence of parallel tubular structures in lymphocytes of patients with hodgkin's disease was investigated for a correlation with either impairment of cellular immunity (measured by dncb-skin test and pha-induced lymphocyte stimulation in vitro) or an increase of antibodies against cytomegalovirus or epstein-barr virus. no correlations were found. statistical analysis revealed antibody titers especially in the group of patients with high percentages of lymphocytes containing the t ...1975169660
immunology of sarcoidosis.the cardinal immunologic changes in sarcoidosis consist of depression of delayed-type hypersensitivity, hyperreactive circulating antibody responses and the kveim-siltzbach skin test phenomenon. depression of delayed-type hypersensitivity is demonstrated by skin tests using tuberculin, mumps, pertussis, trichophytin, oidiomycin, dinitrochlorobenzene and californian keyhole limpet hemocyanin. the cultured lymphocytes from patients with depression of delayed-type hypersensitivity react poorly to p ...1975169693
epstein-barr virus associated with episodes of recurrent tonsillitis.a group of patients with a history of recurrent tonsillitis were observed during an acute episode to determine the cause of the infection. the microbial pathogen that was consistently implicated was the epstein-barr virus. seventeen (65%) of 26 patients exhibited a substantial seroconversion to the early antigen of epstein-barr-virus-infected lymphoblastoid cells (p3hr-1). we conclude that there is a high incidence of tonsillitis associated with the epstein-barr virus. the propensity of the viru ...1975169772
presence of epstein-barr virus nuclear antigen in nasopharyngeal carcinoma biopsies and their derived cultures. 1975169807
t-lymphocyte cell lines derived from patients with acute lymphoblastic leukemia.several lymphoid cell lines with thymus-dependent lymphocyte (t-cell) characteristics have been established from 2 patients with acute lymphoblastic leukemia (all). these cell lines are considered to be leukemic t-cells on the basis of their abnormal chromosome constitution, growth in heterologous animals, ability to form rosettes with sheep red blood cells and the absence of immunoglobulin production, and destruction by antithymus cell sera. sera from patients with leukemia did not react with t ...1975169811
experiments to vaccinate marmoset monkeys against malignant lymphoma.humoral antibodies can be induced in cotton topped (ct) marmosets with killed herpesvirus saimiri (hvs) vaccines. the serum antibodies delayed, but did not prevent, malignant lymphoma in actively and passively immunized monkeys after inoculation with 10(3.8)tcid50 of cell-free hvs. experiments are in progress to determine whether the vaccines are able to protect against smaller challenge dosages of hvs. the immunized monkeys were not resistant against transplantation of tumor cells. infection wi ...1975169814
comparisons of surface markers on herpesvirus-associated lymphoid cells of nonhuman primates and established human lymphoid cell lines.herpesvirus saimiri (hvs)-owl monkey lymphoid cells were found to have high levels of surface receptor for sheep erythrocytes and erythrocytes of 3 other species. these hvs-lymphoid cells lacked a receptor for modified complement. lymphoid cells of one hvs-owl monkey line showed evidence for the presence of surface immunofluorescent staining with anti-kappa chain serum. cells of an established hvs-marmoset lymphoid line had similar surface markers. of 4 established human lymphoid cell lines, all ...1975169815
eb virus: malignant lymphoma in cottontop marmosets following inoculation and recovery of the virus from cells of an experimental tumor maintained in organ culture.the oncogenic potential of epstein-barr virus (ebv) was investigated in cottontop marmosets. neoplasia resembling human malignant lymphomas, reticulum cell sarcoma type, occurred following inoculation of materials containing ebv. one of 4 monkeys that received autologous cells transformed in vitro by ebv developed lymphoma in mesenteric lymph nodes seven and one-half months after inoculation. three of 4 marmosets inoculated with cell-free ebv developed lymphoma. the latent period for given with ...1975169816
detection of epstein-barr virus dna in human tumors.the presence of epstein-barr virus (ebv) dna in biopsies of human tumors was tested by complementary rna (crna) hybridization on membranes and dna-dna reassociation kinetics. dna-dna reassociation kinetics can detect as few as 1 ebv genome in every 50 cells, and the crna method can detect ebv genomes if 2 or more are present in a cell. twenty-three out of 24 african burkitt's lymphoma biopsies, 18 out of 23 african nasopharyngeal carcinomas (npc), and 8 out of 24 other african tumors were positi ...1975169825
dual persistence of epstein-barr viral and type-c viral genomes in nonproducer human lymphoblastoid cells. 1975169826
suppression of production of epstein-barr virus in p3hr-1 cell line by bovine serum. 1975169827
immunological control of virus-induced tumors in primates.cells infected by oncogenic viruses may transform, may develop a latent carrier state, or may be destroyed but understanding of the control of the results of infection is incomplete. even if cells transform, ultimate development of a tumor may be immunologically controlled. for example, cells of some marmoset species transform after infection with rna tumor viruses, and animals react to the transformed cells with cell-mediated and humoral immune responses. both virus specific and cross-reacting ...1975169832
editorial: the several viruses of post-transfusion hepatitis. 1975169948
clinical aspects of viral infections in kidney-graft recipients. 1975170042
oncogenic properties of human viruses. 1975170197
ultraviolet inactivation of epstein-barr virus: effect on synthesis of virus-associated antigens.the relative sensitivity to ultraviolet (uv) light of genome functions of the p3hr-1 strain of epstein-barr virus (ebv) was studied. the formation of viral capsid antigen (vca) appeared to be more sensitive than that of early antigen (ea), while the synthesis of membrane antigen (ma) was most resistant, as seen on examination in the presence of cytosine arabinoside (ara-c). however, the appearance of both vca and ea, but not that of ma, was delayed with uv-irradiated virus, in either the presenc ...1975170209
production by ebv infection of an ebna-positive subline from an ebna-negative human lymphoma cell line without detectable ebv dna.a continuous lymphoma cell line, bjab, derived from the tumour of an exceptional african case of burkitt's lymphoma, has previously been described. unlike 97% of african bl cases studied, neither the original tumour cells nor the cell line contained detectable amounts of ebv (epstein-barr virus) dna, nor did they express the ebv-determined nuclear antigen ebna. the cells of the established line had the characteristics of b-type lymphocytes and they carried receptors for ebv. ebna was induced in ...1975170210
nasopharyngeal carcinoma. x. presence of epstein-barr genomes in separated epithelial cells of tumours in patients from singapore, tunisia and kenya. 1975170215
improved epstein-barr virus immunoglobulin m antibody test.the successful demonstration of epstein-barr virus immunoglobulin m (ebv igm) antibody in human sera has been accomplished to date by at least four groups of workers. many, however, including ourselves, have had difficulty in getting reproducible results with the techniques described. the three-coat technique described by h. schmitz and m. scherer (1972) on both fractionated and unfractioned sera was adopted with minor modifications. the hyland antihuman igm antiserum used in the second coat was ...1975170305
antibodies to epstein-barr virus in patients with cryptococcosis.antibody levels to the epstein-barr virus, the etiological agent for heterophile-positive infectious mononucleosis, have been demonstrated in high titer in a number of lymphomas as well as infectious mononucleosis. recent reports have suggested that the elevated antibody levels to epstein-barr virus may be the nonspecific result of disordered cell-mediated immunity. this study of patients with cryptococcosis was therefore undertaken to examine another disorder of known etiology associated with a ...1975170312
radioimmunoassay for detection of antibodies to epstein-barr virus in human infectious mononucleosis serum specimens.a rapid microradioimmunoassay (ria) technique was adapted for quantitatively measuring antibody titers to antigens occurring in epstein-barr virus (ebv)-infected lymphoid cells. in these experiments two ebv-infected cell lines, hr1k and eb-3, were used as antigen-positive cells and molt-4 was used as the negative control cells. the antibody titers of sera from suspected infectious mononucleosis patients were compared by ria and indirect fluorescent antibody (ifa) methods. as determined by each o ...1975170314
sensitivity of the epstein-barr virus transformed human lymphoid cell lines to interferon.the effect of interferon on expression of epstein-barr virus (ebv) early gene functions was investigated. the 'early antigen' synthesis which follows either ebv superinfection of established lymphoid cell lines or 5'-iododeoxyuridine activation of the intrinsic ebv genomes harboured by these cells could be suppressed with interferon. in contrast, the spontaneous early antigen expression that occurs in a few per cent of the cells in the producer cell lines could not be blocked with interferon. th ...1975170370
spontaneous interferon production and epstein-barr virus antigen expression in human lymphoid cell lines.established human lymphoid cell lines, many of which spontaneously produce interferon, differ in the efficiency by which they allow expression of epstein-barr virus (ebv) lytic functions. six ebv carrying lymphoid cell lines, selected to either be extremely susceptible or very refractory to ebv superinfection, were tested for spontaneous interferon production. only the three cell lines which were poorly superinfectable with ebv were found to produce interferon. these same three lines could not b ...1975170371
infectious mononucleosis: histopathologic aspects.the main histopathologic features of infectious mononucleosis are described. in the lymph nodes, the principal change is the appearance of numerous large pyroninophilic cells (immunoblasts), initially expanding the paracortical zone but later extending throughout the node. similar, large lymphoid cells appear as infiltrates in many other organs and tissues. cells morphologically similar to sternberg-reed cells may be found in the lymph nodes of patients with infectious mononucleosis and other co ...1975170576
new vistas in hodgkin's disease. 1975170577
the role of epstein-barr virus (ebv) in various human disorders. 1975170689
epstein-barr virus antibodies in patients with carcinoma of the nasopharynx and carcinoma of other sites in the head and neck.serum antibody titers to epstein-barr virus were significantly elevated in a group of twenty-three patients with nasopharyngeal carcinoma as compared with eighty-six patients with cancer of other sites in the head and neck and 222 age-matched controls. our findings in this group of patients support the suggestion of an association between the epstein-barr virus and nasopharyngeal carcinoma.1975170838
progress in the application of new immunoenzymatic methods in virology. 1975170847
studies on epstein-barr virus-associated antigens. 1975170849
thymus transplantation. reconstitution of cellular immunity in a four-year-old patient with t-cell deficiency.the case is reported of a 4-year-old girl affected with recurrent infections; anaemia, thrombocytopenia, haemorrhages and hepatosplenomegaly. immunological investigations revealed a defect in cellular immunity related to the thymus-dependent system, hypergammaglobulinaemia (especially of class ige), and very high titres of antibodies against epstein-barr virus (ebv). after foetal thymus transplantation, correction of the immunological defect and significant clinical improvement were noted, as we ...1975171111
nasopharyngeal carcinoma. ix. antibodies to ebna and correlation with response to other ebv antigens in chinese patients.ninety-five sera from chinese npc patients in different stages of the disease, 38 sera from chinese patients with other cancers, and 50 normal chinese sera, were titrated for ebna, vca, ea and complement-fixing (cf/s) ebv-specific antibodies. the geometric mean (gmt) ebna antibody titre of patients with npc at stage i was found to be four times higher than that of normal individuals and increased in parallel with clinical deterioration. antibody titres against ebna did not correlate with either ...1975171226
human leukemia-associated antigens: detection on cells of established lymphoblastoid lines.primate and rabbit antisera to different morphologic classes of human leukemia cells, after appropriate absorptions, detected leukemia-associated antigens present on cultured lymphoblastoid cell lines derived from leukemia patients. the primate antisera distinguished antigens on cells derived from myeloid leukemia patients from those on cells derived from lymphocytic leukemia patients. of particular interest was the fact that antigens of myeloid leukemia, but not of lymphatic leukemia, were dete ...1975171308
epidemiology of cytomegalovirus infection after transplantation and immunosuppression.viral infections and clinical complications were studied during hemodialysis and after renal transplantation. active cytomegalovirus infection developed in 96% of patients after renal transplantation; reactivation of herpes simplex, varicella-zoster, and epstein-barr viruses was found in 35%, 24%, and 0% of patients, respectively. cytomegalovirus viremia developed in 42% of patients an average of two months after renal transplantation, lasted 1.75 (+/- 1.5) months (except in one patient with chr ...1975171315
direct visualization of enveloped epstein-barr herpesvirus in throat washing with leukocyte-transforming activity.mature enveloped virions belonging to the herpes class were found in a concentrated, partially purified specimen of throat washings from a patient undergoing immunosuppressive therapy for prevention of renal homograft rejection. this throat washing contained a high titer of biologically active epstein-barr virus and no other human herpesviruses. epstein-barr virions were not detected in throat washings from patients with mononucleosis that had only low titers of transforming activity.1975171319
a prospective evaluation of heterophile and epstein-barr virus-specific igm antibody tests in clinical and subclinical infectious mononucleosis: specificity and sensitivity of the tests and persistence of antibody.three heterophile antibody tests and a test specific for igm antibody to epstein-barr virus were evaluated during prospective studies of infectious mononucleosis. specificity was judged by the frequency of false-positive results in sera of known qualities taken before illness; except for two patients bled during early, unrecognized illnes,, titers of greater than or equal to 1:40 were detected in 12% by the absorbed sheep red cell test, in 6.7% by the absorbed horse red cell test, and in none by ...1975171321
suppression of spontaneous in vitro transformation of autologous leukocytes by plasma from convalescent and postconvalescent infectious mononucleosis patients.all 14 plasma samples from 13 convalescent and postconvalescent infectious mononucleosis (im) patients suppressed the spontaneous in vitro transformation of autologous leukocytes. in contrast, only 2 of 8 plasma samples from patients with acute im suppressed this transformation. all 7 patients whose blood was tested both in the acute and convalescent or postconvalescent phases of im showed either a conversion in transformation suppression status from negative to positive or an increase in the st ...1975171425
amounts of epstein-barr virus dna in somatic cell hybrids between burkitt lymphoma-derived cell lines.the amounts of epstein-barr virus dna in somatic cell hybrids between human lymphoid cell lines were found to be higher than in the parental cell lines, although the hybrids were not induced to spontaneous virus production.1975171457
cell-mediated immunity to epstein-barr-virus-transformed lymphoblastoid cells in acute infectious mononucleosis.mononuclear peripheral blood leukocytes from 21 patients with infectious mononucleosis and 16 healthy controls were tested in a 51cr-release assay for cytotoxicity against two human lymphoblastoid cell lines derived from the same donor. one line contained the epstein-barr virus (ebv); the other did not. acute-phase leukocytes were significantly more cytotoxic against the ebv-infected cell line than were control leukocytes. mean (+/- s.e.) lysis at a leukocyte-target-cell ratio of 100:1 was 10.6 ...1975171568
hbs-ag-negative hepatitis in a hemodialysis unit: relation to epstein-barr virus.eleven of 40 patients in a hemodialysis unit had clinical or biochemical evidence of hepatitis during a five-week period. the clinical disease was mild, being limited solely to dialysis patients. epidemiologic investigation indicated that the incubation period was between 17 and 35 days and that 10 of 11 patients had been exposed to a single venous-pressure monitor before onset. dried blood and evidence of blood reflux up the venous-pressure gauge suggested that cross-contamination of the blood ...1975171573
the epstein-barr virus and neoplasia. 1975171578
enhanced representation of hl-a antigens on human lymphocytes after mitogenesis induced by phytohemagglutinin or epstein-barr virus.the amount of hl-a antigens present on the peripheral blood lymphocytes of a single human donor was increased about 11-fold after stimulation with phytohemagglutinin and 36-fold after transformation with epstein-barr virus. this increase applied to all four hl-a specificities of these cells. the response to phytohemagglutinin was dependent on dose and was first observed at 12 hr of incubation. measurements of the amount of surface membranes by geometry, by radioiodinatable surface proteins, and ...1975171657
comparison between growth characteristics of an epstein--barr virus (ebv)-genome-negative lymphoma line and its ebv-converted subline in vitro.the gc-bjab cell line, which carries the epstein--barr virus (ebv), was derived from an ebv-genome-negative lymphoma line (bjab) by ebv infection in vitro [g. b. clements, g. klein, and s. povey (1975) int. j. cancer, in press]. both lines grow at a similar rate at 37 degrees but they differ at other temperatures. bjab grows well at 34 degrees, 37 degrees, and 39 degrees. gc-bjab grows at 37 degrees and 39 degrees, but grows poorly at 34 degrees. at 37 degrees, gc-bjab cultures can be maintained ...1975171663
growth of permanent lymphoid cell cultures from human source: tenth anniversary.the authors developed 52 permanent lymphoid cell cultures from various human sources, mainly from neoplastic lymphoproliferative disease. these cultures are reviewed against the background of similar cultures obtained in several other laboratories. the peculiar features of these cultures are: 1) the phenomenon of lymphoblastoid transformation; 2) the production of both globulins and mediators of delayed hypersensitivity; 3) the presence of herpes type virus as related to etiology or autoimmunity ...1975171791
[genetic manipulation of eukaryotic cells]. 1975171811
[immunovirological factors in epitheliomas of the rhinopharynx].the epidemiology of nasopharyngeal carcinoma (npc) suggests the intervention of genetic factors. these factors may be represented by a specific hl-a pattern (leucocyte antigens) as found in chinese npc patients (deficit of antigens corresponding to the second sublocus and discovery of a new antigen, named sin-2). the association between npc and a herpesvirus (the ebv) is based on elevated antibodies against a variety of ebv coded antigens, on cell mediated immune reactions, and on the regular pr ...1975172172
antibodies to epstein-barr virus in patients with hodgkin's disease and leukemia.sera from 67 hodgkin's disease patients, 71 leukemia patients, and 186 healthy subjects were tested for antibodies to epstein-barr (eb) viral antigens by immunofluorescence methods. in both disease categories, in particular hodgkin's disease patients, levels of antibodies to the viral capsid antigen (ebv-vca) and mgt were higher than in the healthy controls. significantly higher titers were found in jewish patients of asian-african origin, as compared to jews of european origin, with arab patien ...1975172216
[occult nasopharyngeal carcinoma (author's transl)].clinical and histological examination of a cervical lymph node, removed from a 15-year-old boy, revealed nasopharyngeal carcinoma as the probable diagnosis. but the decisive diagnostic pointer was a markedly raised epstein-barr virus antibody titre, which gives the diagnosis at a time when there may not as yet be clinical evidence of carcinoma. serial serum titre determinations are of prognostic value: with increasing spread of the tumour there is a rise in antibody titre. autopsy confirmed that ...1975172307
etiological spectrum of post-transfusion hepatitis.frequent occurrence of post-transfusion hepatitis continues despite screening for australia antigen in blood before transfusion and elimination of commercial donor sources. the majority of these cases appears unrelated to hepatitis b virus. preoperative, acute, and convalescent serra were screened for evidence of hepatitis b, hepatitis a, epstein-barr, and cytomegalovirus exposure in 34 cardiac surgery patients developing post-transfusion hepatitis postoperatively. four patients showed evidence ...1975172400
origin of lymphoid lines established from mixed cultures of cord-blood lymphocytes and explants from infectious mononucleosis, burkitt lymphoma and healthy donors.lymphocytes were explanted from ebv-seropositive donors including peripheral blood of infectious mononucleosis patients, healthy donors and ebv-genome-carrying cells from burkitt lymphoma (bl) biopsies or nude mouse-passaged, bl-biopsy-derived lines. the explanted cells were mixed with fresh cord-blood lymphocytes from mice of the opposite sex. in all categories of derived lines, cord-blood cell progeny was predominant, as judged by the sex marker and other associated markers. only one bl biopsy ...1975172457
sensitive method to detect non-complement-fixing antibodies to epstein-barr virus nuclear antigne.the anti-complement immunofluorescence (acif) test was modified to detect non-complement-fixing antibodies to epstein-barr (eb) virus nuclear antigen (ibna). these ebna antibodies belong to the immunoglobulin classes iga and igg. in our method anti-human gamma-globulin (ahg) was bound to the ebna antibodies before the complement was added. if only non-complement-fixing antibodies are present the complement can be fixed to the ahg. within only a few weeks of the onset of infectious mononucleosis ...1975172458
spontaneous interferon production and epstein-barr virus antigen expression in two human lymphoid cell lines and their somatic cell hybrids.two human lymphoid cell lines, raji and namalwa, and seven somatic cell hybrids between these two cells were tested for spontaneous interferon production. namalwa and three of the hybrids produce interferon while the four other hybrids, like raji, do not. though the namalwa line is relatively resistant to superinfection with the epstein-barr virus (ebv), all hybrids are as susceptible to ebv as the raji parent. high extracellular concentrations of interferon likewise had little effect on ebv sup ...1975172473
[clinical examination of eb virus infection (author's transl)]. 1975172670
absence of infectious epstein-barr virus in blood in acute infectious mononucleosis. 1975172793
persistence of cmv genome in lymphoid cells after congenital infection. 1975172796
growth and differentiation in culture of leukemic leukocytes from a patient with acute myelogenous leukemia and re-identification of type-c virus.conditioned medium from a culture of whole human embryo cells stimulated prolonged exponential growth in suspension culture of leukocytes from a patient with acute myelogenous leukemia. ten to 20% of the cultured cells consistently differentiated into mature granulocytes including neutrophils, eosinophils, and basophils. the proportion of lymphocytes declined after culturing, and tests for epstein-barr virus antigens were negative. an abnormality of a g group chromosome was observed in some meta ...1975172897
epstein-barr herpesvirus and infectious mononucleosis. 1975172973
virologic studies in rheumatoid arthritis.comparison of virus antibody titers in jra and matched controls failed to implicate rubella, measles, parainfluenza type 1 or epstein-barr viruses. neither rna viruses or mycoplasma were detected in rheumatoid synovial cell cultures by 3h-uridine incorporation. mycoplasma were not isolated from rheumatoid synovial membranes or cell cultures using the large specimen-broth culture procedure. the balance of available evidence, including our own, suggests that mycoplasma are not involved in ra. viru ...1975173010
stimulation of normal lymphocytes with autologous lymphoid cell lines: properties of derived killer cells.lymphocytes from normal adults, with or without serological signs of previous epstein-barr virus (ebv) infection, could be stimulated to proliferate and produce killer cells by incubation with autologous ebv-genome-positive lymphoid cell lines (lcls). the stimulated cells were most probably of t-cell origin, although at the peak of stimulation many of them lacked the sheep erythrocyte marker. direct effector-target cell contact was necessary for lysis to occur. the cytotoxicity of autologously s ...1975173017
carcinoma of the nasopharynx in children with carcinoma of the nasopharynx have been treated and followed for five years. in this group of patients there were no cranial nerve palsies or radiographic evidence of destruction of the base of the skull. four of the seven patients are living and well. the development of lymphoepithelioma in two siblings is of great interest from an environmental and genetic point of view. the older sibling developed the clinical manifestations of the tumor at 12 years of age and four years la ...1975173235
tubuloreticular structures in human lymphoid cell lines. a cell biological study.human lymphoid cell lines were studied as an experimental model for the spontaneous or induced occurrence of tuburloreticular structures (trs). it was possible to induce trs after culturing the eb-3 cell line with 20 mug/ml bromodeoxyuridine (brudr) during 96 h. starvation, culturing at lower temperature (32 degrees) or inhibition of dna synthesis did not give rise to the production of trs. the response to brudr could be blocked with 60 mug/ml thymidine but not with 60 mug/ml deoxycytidine. the ...1975173383
letter: acute leukemia with burkitt's tumor cells. 1975173442
childhood hodgkin's disease in israel. a study of 17 cases.a study of 17 children suffering from hodgkin's disease (h.d.) is presented. the special ethnic distribution with predominance in arab and oriental jewish children is noted. a high incidence of mixed cellularity histology in arab children is emphasized. a conservative approach to therapy, i.e. extended field regional radiotherapy only, resulted in a long first complete remission period in lymphocyte predominance and nodular sclerosis h.d. stages ia and iia. all children in stage iiib and iv were ...1975173461
anti-epstein-barr virus antibody in patients with cancer of various sites and control groups.sera collected from patients with nasopharyngeal carcinoma (npc, 321 cases), cancer of the other sites (297 cases), diseases of the ear, nose and throat (64 cases) and neighborhood controls matched for age and sex (817 cases) were titrated for antibodies to epstein-barr virus (ebv) by the indirect immunofluorescence antibody technique. high anti-ebv antibody titers (greater than or equal to 1:640) were noted in 55% in patients with npc but only less than 31% were noted for the other 3 groups. th ...1975173499
an improved specific igm antibody assay for epstein-barr virus.a new method for detecting specific igm antibodies to the epstein-barr virus using indirect fluorescence is presented. the procedure is simple and reproducible, and of great value for the diagnosis of an acute infection. the number of positive cells is higher than in the epstein-barr igg test. it would seem that it is possible to detect a positive igm-specific antigen with this new test.1975173658
epidemiologic study of a leukocyte-transforming agent in a general population.studies by others have demonstrated a leukocyte-transforming agent(s) (lta) in the oropharyngeal secretions of a significant number of individuals with epstein-barr virus-associated infectious mononucleosis, cancer, and, to a lesser extent, an outpatient population. this present study determines by systematic sampling the prevalence of lta in 27 families of a semirural community. throat swab inoculums from three of 54 adults and none of 44 children induced transformation of umbilical cord lympho ...1975173738
[antibody to epstein-barr virus in head and neck tumors (author's transl)]. 1975173824
in vitro evaluation of cell-mediated immunity to epstein-barr herpesvirus by cell migration inhibition tests.migration of peripheral leukocytes in samples from sensitized [epstein-barr virus (ebv) antibody-positive] humans was greatly inhibited when challenged by antigen prepared from ebv-producing p3hr-1 cells but not by antigen prepared from ebv-nonproducing raji cells, ebv-negative human fibroblasts, or epithelial cells. such inhibition was not observed when peripheral leuocytes from subjects or neonates not sensitized to ebv were challenged. similar results were obtained in a two-stage test when th ...1975173867
[juvenile tonsillectomy and leukemia].among 305 children with leukemia, between one and fourteen years old, authors found 8 and in a comparative group ("individual matched pairs") 40 children with removed tonsils. the difference (2.62% tonsillectomized children in the leukemia-group, 13.11% in the comparative group) is highly significant. the frequency of tonsillectomy in childhood in germany corresponds to the percentage of 13.11%. it has to be discussed, if there exists a causality between the state of the tonsils and the frequenc ...1975173947
letter: immune mechanisms in infectious mononucleosis. 1976173991
surface marker characteristics and epstein-barr virus studies of two established north american burkitt's lymphoma cell lines.tumor cell lines have been established in continuous culture from two north american burkitt's lymphomas. the su-amb-1 line, derived from a patient with low serum antibody titers to epstein-barr virus (ebv), was devoid of ebv genomes by the reaction for ebv-associated nuclear antigen (ebna), could not be induced to express ebv antigens, and was highly refractory to ebv superinfection. conversely, the su-amb-2 cell line, derived from a patient with "african type" serology, yielded a positive ebna ...1976174098
viral-induced thymidine kinase isozymes. 1975174152
concanavalin a receptors and other cell surface and antigenic characteristics of continuous lymphoblastoid cell lines derived from patients with hodgkin's disease and other malignant lymphomas. 1975174153
biological properties of the epstein-barr virus and its possible role in human malignancy. 1975174156
consideration of the role of viruses in human cancer. 1975174158
[oncogenic viruses and carcinogenesis in man]. 1975174160
effect of the host cell on the maintenance and replication of epstein-barr virus. 1976174288
recent studies with eb virus. 1975174330
[use of demographic method for the study of adenoviruses and herpesviruses in a closed infants' community]. 1975174823
[different types of oncogenic viruses associated with hemoblastosis in monkeys]. 1975174875
epstein-barr virus-specific iga serum antibodies as an outstanding feature of nasopharyngeal carcinoma.stimulated by a report on elevated iga levels in nasopharyngeal carcinoma (npc), we tested a total of 372 sera from patients with npc, other carcinomas of head and neck or elsewhere, burkitt's lymphoma (bl), infectious mononucleosis (im) or healthy controls. the sera were titrated in indirect immunofluorescence tests for iga antibodies to epstein-barr virus (ebv) capsid antigen (vca) and to the diffuse (d) or restricted (r) components of the ebv-induced early antigen (ea) complex. the results pr ...1976175020
comparative studies of epstein-barr virus strains from ghana and the united states.a high incidence of oropharyngeal excretion of epstein-barr virus (ebv) has been observed in african children with burkitt's lymphoma (bl) (48%) and matched controls (45%). this compares with an incidence of 77% in american patients with infectious mononucleosis (im) and 13% in age-matched controls. cross-neutralization tests between ebv strains derived from bl and im patients and their sera failed to detect differences in the major neutralizing antigenic components. cord-blood lymphocytes trans ...1976175023
immunity to antigens associated with a cell line derived from nasopharyngeal cancer (npc) in non-chinese npc patients.delayed hypersensibility to antigens derived from four lymphoid cell lines was measured in 27 non-chinese patients with nasopharyngeal cancer (npc) and 63 non-npc cancer patients. of the npc patients, 17/27 (63%) had a positive skin test response to antigens derived from hkly-28, a lymphoid cell line which was developed from an npc biopsy. only 10/51 (20%) and 1/13 (8%) patients with solid tumors and hematopoietic malignancies, respectively, had positive skin test responses to hkly-28. positive ...1976175025
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