| monoclonal antibodies in the therapy of experimental neonatal group b streptococcal disease. | group b streptococcal (gbs) infections continue to be a major cause of morbidity and mortality in human neonates. this has led a number of investigators to explore the role of immunotherapy in the treatment of neonatal gbs disease. in early studies, we showed that intravenous immune globulin (ivig) offered some protection against less virulent strains of gbs in a neonatal rat model of disease. against more virulent strains, which produce an excess of sialic acid-containing type-specific antigen, ... | 1992 | 1728992 |
| gold-specific t cells in rheumatoid arthritis patients treated with gold. | gold-specific t lymphocyte clones were isolated from a patient with rheumatoid arthritis who developed delayed type hypersensitivity reactions to gold. all of the isolated t cell clones required histocompatible antigen presenting cells as well as gold for induction of proliferation. using a panel of hla-homozygous epstein barr virus-transformed b (ebv-b) cells and anti-hla antibodies, the clones were shown to recognize gold in the context of dr1 molecules. gold recognition did not require active ... | 1992 | 1729275 |
| the molecular defect of ferrochelatase in a patient with erythropoietic protoporphyria. | the molecular basis of an inherited defect of ferrochelatase in a patient with erythropoietic protoporphyria (epp) was investigated. ferrochelatase is the terminal enzyme in the heme biosynthetic pathway and catalyzes the insertion of ferrous iron into protoporphyrin ix to form heme. in epstein-barr virus-transformed lymphoblastoid cells from a proband with epp, enzyme activity, an immunochemically quantifiable protein, and mrna content of ferrochelatase were about one-half the normal level. in ... | 1992 | 1729699 |
| establishment and characterization of a thymic carcinoma cell line (ty-82) carrying t(15;19)(q15;p13) chromosome abnormality. | a new human cell line, designated ty-82, was established from the pleural effusion of a 22-year-old woman with undifferentiated thymic carcinoma. this cell line consisted of primitive cells that were positive for alpha-naphthyl butyrate esterase and acid phosphatase. the cells were shown to express epithelial membrane antigen, but were completely negative for cytokeratin, carcinoembryonic antigen, glial fibrillary acidic protein, desmin, s-100 protein, lysozyme, leu-7, hla-dr (ia), leukocyte com ... | 1992 | 1730520 |
| hepatitis b virus (hbv)-specific cytotoxic t-cell (ctl) response in humans: characterization of hla class ii-restricted ctls that recognize endogenously synthesized hbv envelope antigens. | in this study, we show that cd4+, hepatitis b virus (hbv) envelope-specific t-cell clones produced by stimulation with a particulate antigen preparation are able to recognize and kill not only autologous antigen-presenting cells incubated with exogenous hbv envelope antigens but also autologous hla class ii-positive cells expressing endogenously synthesized hbv envelope antigens following infection with recombinant vaccinia viruses or transfection with recombinant epstein-barr virus expression v ... | 1992 | 1731098 |
| use of photosensitive, antibody directed liposomes to destroy target populations of cells in bone marrow: a potential purging method for autologous bone marrow transplantation. | liposomes containing the photosensitive dye sulphonated aluminium phthalocyanine (alspc) were coupled to polyclonal sheep anti-mouse-ig antibody and bound to cells coated with specific mouse monoclonal antibody. when illuminated with red light, the alspc in the liposomes was activated to produce singlet oxygen and the antibody and liposome targeted cells were destroyed. dw-bcl cells (an epstein barr virus immortalised b-cell line) were targeted with an anti-b-cell antibody (8a) and killed specif ... | 1992 | 1733442 |
| production of heterohybridomas secreting autoreactive and polyreactive human monoclonal antibodies. | peripheral blood lymphocytes from two polytransfused renal dialysis patients were transformed by epstein-barr virus, fused to a heteromyeloma and cloned. eight human monoclonal antibodies from the resulting clones were tested for their binding to a variety of antigens by elisa, indirect immunofluorescence and immunoblotting. antigens tested included b-cell lines, t and b lymphocytes, red blood cells, chronic lymphocytic leukaemic b cells, igg, ssdna, dsdna, histones, nucleoprotamine, sperm nucle ... | 1992 | 1734494 |
| a chimera of ebna1 and the estrogen receptor activates transcription but not replication. | dna replication from the plasmid origin of replication of epstein-barr virus requires one viral protein, ebna1. this protein also acts as a transcriptional activator. mutational analyses of ebna1 have led to the conclusion that it supports transcription and dna replication similarly. such analyses have not probed the dna-binding domain of ebna1. to test whether domains of ebna1 specifically required for either transcription or replication lie within its dna-binding domain, we constructed a funct ... | 1992 | 1738208 |
| lymphoproliferative diseases of uncertain classification. | in spite of the recent progress in the biology and histogenesis of the lymphoproliferative diseases, many doubts still remain about their possible evolution. on the one hand, many cases have been studied showing a benign course in spite of a typical monoclonal phenotype; on the other, some clinically aggressive forms of lymphoproliferation prove to be polyclonal throughout their course, even when checked with highly sophisticated techniques. in this article we have reviewed, in both clinical and ... | 1991 | 1742152 |
| relationship between dna cross-links, cell cycle, and apoptosis in burkitt's lymphoma cell lines differing in sensitivity to nitrogen mustard. | we surveyed 11 burkitt's lymphoma cell lines for chemosensitivity to nitrogen mustard (hn2) in order to determine whether any simple correlates to cytotoxic response might be revealed. the lines tested varied over a 5-fold range in concentration of hn2 required to inhibit tumor cell growth by 50%. drug sensitivity correlated neither with continental origin of tumor, growth fraction, presence of epstein-barr virus, nor with the precise locations of (8;14) translocation breakpoints. furthermore, c ... | 1991 | 1742728 |
| nasopharyngeal carcinoma. a comparative study. | a comparative study of ghanaian and saudi hospital cases of nasopharyngeal carcinoma (npc) shows a higher occurrence among younger subjects in the ghanaian group, with the peak age incidence in the saudi group being similar to those reported from other parts of the world. in view of the known high prevalence of burkitt's lymphoma among ghanaian children, it is suggested that the much higher proportion of younger ghanaian subjects with npc may be related to early exposure to the epstein-barr viru ... | 1991 | 1750131 |
| severe herpes simplex virus hepatitis following autologous bone marrow transplantation: successful treatment with high dose intravenous acyclovir. | a 17-year-old male patient with t-cell type lymphoblastic lymphoma in complete remission underwent high dose chemotherapy (busulfan 16 mg/kg and cyclophosphamide 120 mg/kg) followed by autologous bone marrow transplantation (abmt). the patient had been taking oral acyclovir (200 mg x 5) daily from seven days prior to the abmt (day -7). on day +24, he complained of epigastralgia and general malaise, and the next day his got and gpt rose to 570 u/l and 397 u/l, respectively. although he had no muc ... | 1991 | 1753418 |
| t-cell immunotherapy of cancer. | in animal systems, complete and permanent eradication of tumours can be achieved by adoptive transfer of mhc-restricted t cells, combined with il2. in certain types of human cancer (melanoma and perhaps renal cell carcinoma), tumour-specific t cells are probably the therapeutically most active cells among lak or til cells. to prove these points, it is necessary to conduct trials with cloned tumour-specific t cells. other potentially immunogenic tumors are cervical carcinoma, associated with huma ... | 1991 | 1754715 |
| a review of forty-five years study of hiroshima and nagasaki atomic bomb survivors. biochemical genetics study. | after 40 years of studies on the children of atomic bomb survivors and a suitable control population in which no statistically significant genetic effects of a-bomb radiation were observed, two new projects have been initiated in order to detect mutations in the dna and rna molecules. permanent cell lines from peripheral b-lymphocytes from approximately 500 families composed of exposed parents and their children and approximately 500 control families are being established by epstein-barr virus t ... | 1991 | 1762126 |
| viruses in the aetiology of oral carcinoma? examination of the evidence. | this paper examines the evidence for an aetiological role for viruses in the development of oral carcinoma. several viruses have been sought in oral cancer and evidence found for some, particularly herpes simplex and human papillomavirus. however, the evidence also suggests that these viruses are ubiquitous agents and a number of criteria must be met before these potentially oncogenic agents can reliably be implicated in human carcinogenesis. in contrast, there is no evidence that viruses such a ... | 1991 | 1772858 |
| nasal polyposis: in search of a viral etiology using dna hybridization. | the etiology of nasal polyps is unknown. numerous theories have been proposed, with allergic and infectious causes predominant. a viral theory was postulated almost 40 years ago. in a pilot project, we studied 15 subjects comprising six controls and nine patients with nasal polyps. no known viruses were cultured from the nasal polyp tissue or nasal mucosal biopsies from the control subjects. dna hybridization for epstein-barr virus, herpes simplex virus, adenovirus type v, and chlamydia trachoma ... | 1991 | 1774797 |
| the primary structure of plasmodium falciparum dna polymerase delta is similar to drug sensitive delta-like viral dna polymerases. | we report the isolation and sequencing of genomic dna clones that encode the 1094-amino acid catalytic subunit of dna polymerase delta from the human malaria parasite plasmodium falciparum. protein sequence comparison to other dna polymerases revealed the presence of six highly conserved regions found in alpha-like dna polymerases from different prokaryotic, viral, and eukaryotic sources. five additional regions of amino acid sequence similarity that are only conserved in delta and delta-like dn ... | 1991 | 1775172 |
| bilateral iris tumors in an immunosuppressed child. | a seven-year-old iatrogenically immunosuppressed girl developed simultaneous bilateral iris tumors associated with granulomatous iridocyclitis, splenomegaly, and an immunoblastic pleocytosis. serologic studies suggested a current active epstein-barr virus infection. tumor biopsy showed a polyclonal plasmacytoid lymphoproliferative process, establishing the diagnosis of posttransplant lymphoproliferative disorder. low-dose local irradiation produced rapid and complete iris tumor regression with r ... | 1991 | 1776125 |
| differential c-myc protein expression in burkitt's lymphomas and ebv-transformed lymphoblastoid lines. | the levels of c-myc protein expression in three types of epstein-barr virus (ebv) transformed human b-cell derived lines were examined with an elisa assay. six independently maintained sublines of the same ebv-transformed pro-b-cell line (fleb-14), six b-cell lines (lcl) and six burkitt's lymphoma lines (bl) were compared. the average amount of c-myc protein, calculated from at least three independent tests on each line, differed between the three groups. expressed in relative units, the ratio o ... | 1991 | 1782076 |
| intrathoracic carcinoma in an 11-year-old girl showing a translocation t(15;19). | we report here a rare case of intrathoracic undifferentiated carcinoma in an 11-year-old girl. the biopsy tissue stained with antibodies against cytokeratin and carcino-embryonic antigen. the same staining was obtained using a cell line, per-403, which was derived from the tumor. dna from per-403 cells was tested for the presence of epstein-barr virus genes, yielding a negative result. the cytogenetic analysis found a translocation t(15;19) (p12;q13), which has not previously been described in a ... | 1991 | 1785673 |
| depressed ability of patients with melanoma or renal cell carcinoma to generate adherent lymphokine-activated killer cells. | adherent lymphokine-activated killer (a-lak) cells, selected from peripheral blood lymphocytes (pbl) of normal human donors by adherence to plastic, and cultured in the presence of interleukin 2 (il-2), are highly enriched in cd3-cd56+ natural killer (nk) cells. these il-2-activated nk cells proliferate extensively upon further culture in conditioned medium containing il-2. in contrast, we previously found that with pbl of some patients with advanced cancer, the same procedure often failed to yi ... | 1991 | 1790141 |
| regulation of lymphocyte blastogenesis and antibody production by soluble factor released by a human b-lymphoblastoid cell line. | yam 1b, a human b lymphoblastoid cell line, spontaneously produced an immunoregulatory factor, which suppresses blastogenesis and antibody formation by human lymphocytes. the yam 1b cells, which were derived from the peripheral blood of an adult t-cell leukemia patient, have been established and maintained in our laboratory since 1985. this cell line expressed mature b-cell surface antigens including surface immunoglobulin m (igm), cd23, and hla-dr; had cytoplasmic igm; and secreted small amount ... | 1991 | 1790307 |
| anti-idiotypic immunity as a potential regulator in myeloma and related diseases. | in this paper some recent and partly preliminary results on anti-idiotypic immunity against clonal b cells in human monoclonal gammopathies are summarized. b cell lines producing antibodies to idiotypic determinants on autologous monoclonal immunoglobulin could be propagated after activation with epstein-barr virus of peripheral blood lymphocytes from patients with mgus and mm clinical stage i but not from untreated persons with advanced mm. blood t lymphocytes from patients with mgus and walden ... | 1991 | 1793208 |
| incidence of toxoplasmosis in patients with glandular fever and in healthy blood donors. | the differential diagnosis of the clinical syndrome of glandular fever may include epstein-barr virus, cytomegalovirus and toxoplasma gondii infection. some general practitioners and clinical laboratories choose to perform serological investigations for toxoplasmosis in all patients with glandular fever, who have negative paul-bunnell test results. the validity of this approach was assessed by a comparison of the incidence of toxoplasmosis in healthy blood donors and in a group of patients with ... | 1991 | 1793647 |
| involvement of 3p deletions in sporadic and hereditary forms of renal cell carcinoma. | deletions of the short arm of chromosome 3 and associated allele losses have been reported in the majority of sporadic renal cell carcinomas (rcc). on the basis of the combined cytogenetic and molecular data, it is reasonable to assume that a putative rcc locus, which contributes to tumor development by its loss, is located telomerically of the d3f15s2 site. using h3e4, a d3f15s2-specific probe, we have isolated a cdna clone (cl.4-2), and a sequence comparison revealed that the cdna clone corres ... | 1991 | 1797089 |
| production of human monoclonal antibodies against hiv-1 peptides. | protocols were evaluated in an attempt to produce human monoclonal antibodies (humab) specific for the human immunodeficiency virus type 1 (hiv-1). the first series of experiments involved in vitro immunization of normal human peripheral blood lymphocytes (pbl) with peptides c57 (hiv-1 strain iiib clone bh10 gp 120 amino acids 324-338: gnmrqahcnisrakw) followed by either fusion to mouse/human heterohybrids or transformation with epstein barr virus (ebv). using the hybridoma technology, three igm ... | 1991 | 1797270 |
| human quadriceps strength and fatiguability in patients with post viral fatigue. | quadriceps isometric strength, activation and fatiguability were measured in 11 patients with symptoms of fatigue three months after glandular fever or a glandular fever-like illness. predicted normal and lower limits of normal muscle strength were calculated from height and age. these measures and the fatigue index were compared with a group of healthy students of similar age. two of the patients were unable to activate fully their muscles. after allowing for this inhibition the group mean (sd) ... | 1991 | 1800667 |
| functional t cell subpopulations responsible for hyposecretion of il-2 in patients with systemic lupus erythematosus. | the ability of t cells to secrete il-2 in patients with systemic lupus erythematosus (sle) was investigated. in patients with sle, impaired il-2 production by peripheral blood lymphocytes stimulated with mitogens is well known. in this paper, we report that purified t cells stimulated with mitogens, in the presence of epstein-barr virus transformed b cells (b-lcl) as an accessory cell, however, could secrete a large quantity of il-2 as much as normal t cells. in order to study this potential cap ... | 1991 | 1802493 |
| a rabies-specific human monoclonal antibody that protects mice against lethal rabies. | according to a recommendation from who (world health organisation) for prevention of a possible rabies infection, active vaccination has to be combined with application of immunoglobulin to get a fast protective effect. at present, preparations of purified human or equine rabies-specific immunoglobulin are used. we have generated a human rabies-specific monoclonal antibody (humab) by immortalization of human b-cells with epstein barr virus (ebv), followed by fusion with a mouse myeloma cell. the ... | 1991 | 1804770 |
| paucity of humoral response in patients to glioma-associated antigen(s): antigen localization by immunofluorescence. | xenogeneic immunization of freshly-prepared human glioma extracts into goats has yielded a polyclonal antiserum, which after multiple absorptions specifically identifies antigenic entities only in glioma extracts, and not in appropriate controls, both by radioimmunoassays (rias) and western immunoblots. the results from the absorbed polyclonal antiserum have been confirmed by the successful generation of six stable murine monoclonal antibodies (mabs) which recognize a subset of the same antigens ... | 1991 | 1805570 |
| polyreactivity of human monoclonal antibodies: human anti-rh monoclonal antibodies of igm isotype are frequently polyreactive. | the specific aim of this study was to characterize human anti-rh monoclonal antibodies cross-reacting with self-antigens. we studied supernatants from man-mouse hybridomas and from lymphoblastoid cell lines. man-mouse hybridomas were established by fusion of peripheral blood lymphocytes from healthy individuals recently immunized against rh alloantigens, with mouse myeloma (or man-mouse heteromyeloma) cell lines. lymphoblastoid cell lines were produced by epstein-barr virus induction of lymphocy ... | 1991 | 1807061 |
| partial amino acid sequence of the light chain of human anti-rh(d) monoclonal antibody h2d5d2f5. | antibody h2d5d2f5 is a human monoclonal anti-rh(d) igg1 (lambda) produced by epstein-barr virus-immortalized b lymphocytes from a healthy donor. the complete amino acid sequence of the light (l) chain, with the exception of positions 94-97, was determined by edman degradation of the intact chain, containing 30 residues, and derived tryptic and thermolytic peptides. sequences of the peptides were aligned by comparison with the sequences of previously reported l chains. h2d5d2f5 l chain belongs to ... | 1991 | 1812484 |
| demonstration of the induction of apoptosis (programmed cell death) by tetrandrine, a novel anti-inflammatory agent. | tetrandrine, a bisbenzylisoquinoline alkaloid, was found to cause death of malignant lymphoid and myeloid cells but not of epstein-barr virus-transformed lymphoblastoid cells. the death took the form of apoptosis (programmed cell death), the nature of the process being confirmed by dna gel electrophoresis and electron microscopy. the induction of apoptosis by tetrandrine was much more rapid in cem-c7 cells (4 h) than in the same cells treated with glucocorticoids (40 h), and did not require de n ... | 1991 | 1814849 |
| the combined treatment of human peripheral blood mononuclear cells with thymolymphotropin and interleukin 2 increases ppd-driven t-cell proliferation and il-2 induced cellular cytotoxicity against hiv-infected cells. | two in vitro systems (the dna synthetic response to mycobacterial antigens and cytotoxicity against lymphoid cells) were used to analyse the effect of thymolymphotropin (tlt) on peripheral blood mononuclear cells (pbmc). purified protein derivative of mycobacteria (ppd)-driven t-cell proliferation in low-responder donors was increased by the combined treatment with tlt and suboptimal doses of recombinant interleukin 2 (il-2). similarly, the activities of natural killer (nk) cells and lymphokine- ... | 1991 | 1814852 |
| bi-phenotypic t(9;22)-positive leukemia cell lines from a patient with acute leukemia: nalm-20, established at the onset; and nalm-21, nalm-22 and nalm-23, established after relapse. | four leukemia cell lines; nalm-20, established at the onset of leukemia and nalm-21, -22 and -23 established at the relapse of the disease were found to be t(9;22)-positive leukemia lines having the biphenotypic feature of b cell and myeloid cell characteristics. in addition, a polyclonal epstein-barr virus-transformed normal b cell line, b250, was established from the peripheral blood at the onset of the disease. | 1991 | 1817649 |
| epstein-barr virus serological markers for nasopharyngeal carcinoma in thailand. | the present study reports on the prevalence of specific iga and igg antibodies to ebv viral capsid antigen in nasopharyngeal carcinoma (npc) patients with different histological types of carcinoma and their age-matched controls by the indirect immunofluorescence test, using the b-95-8 lymphoblastoid cell line as source of viral capsid antigen. ebv specific igg was found in almost all the study cases, and antibody titers were significantly higher in the npc patients than in non-cancer controls. g ... | 1991 | 1818381 |
| establishment of idiotype bearing b-lymphocyte clones from a patient with monoclonal gammopathy. | b-lymphocyte clones bearing an idiotypic ig structure, which could also be found on the serum m-component, have been isolated from peripheral blood of a patient with monoclonal gammopathy (mg) using an immune-rosetting technique and limiting dilution. the majority of the cells had the surface phenotype id+/cd19+/cd20+/cd21+ or id+/cd19+/cd20+/cd21-. some of the clones originated from spontaneous outgrowth of lymphocytes and some were derived from in vitro epstein-barr virus-infected lymphocytes. ... | 1991 | 1824258 |
| growth of primary t-cell non-hodgkin's lymphomata in scid-hu mice: requirement for a human lymphoid microenvironment. | we reasoned that the scid-hu mouse could provide an appropriate lymphoid or stromal microenvironment to support the growth of primary human lymphoma. heterotransplantation of nine cases of primary t-cell non-hodgkin's lymphoma (nhl) into untreated scid mice and scid mice reconstituted with human fetal thymus, spleen, and liver (scid-hu) resulted in the development of lymphoid tumors in five (56%) cases. two clonal t-cell nhl grew after a mean of 90 days after injection of primary lymphoma cell s ... | 1991 | 1824259 |
| immunologic parameters in early-stage hiv-seropositive subjects associated with vaccine responsiveness. | twenty-one asymptomatic hiv-seropositive subjects and 20 hiv-seronegative controls were assessed for their serologic response to multiple live attenuated viral, protein (toxoid), and polysaccharide vaccine antigens. extensive in vivo and in vitro immunologic evaluations were performed. factors predictive of immunogen responsiveness by hiv-seropositive patients were found by correlating vaccine responses with results of t- and b-cell functional assays. eleven hiv-seropositive patients (hiv nonres ... | 1991 | 1824786 |
| follicular dendritic cells contain a unique gene repertoire demonstrated by single-cell polymerase chain reaction. | follicular dendritic cells (fdcs) form a dense network between b cells within the germinal center and are thought to be an important component of this b-cell microenvironment. previous immunophenotypic studies have been inconclusive in determining the cellular origin of fdcs. gene coexpression within individual and highly enriched fdcs was determined using polymerase chain reaction. fdcs contain a very restricted mrna pattern with high levels of message for the c3d receptor (cr2, epstein barr-vi ... | 1991 | 1825182 |
| a subset of gamma delta lymphocytes is increased during hiv-1 infection. | the gamma delta t cell receptor (tcr) lymphocytes constitute 3-10% of human peripheral blood lymphocytes. only a very small fraction of these cells is recognized by the delta tcs1 monoclonal antibody, directed against the v delta 1 chain of the receptor. we describe the immunological, virological and clinical data of a small group of seropositive subjects having high levels of gamma delta tcr t cells in the peripheral blood. our flow cytometric studies show that most of these cells belong to the ... | 1991 | 1825186 |
| tct.1, a target molecule for gamma/delta t cells, is encoded by an immunoglobulin superfamily gene (blast-1) located in the cd1 region of human chromosome 1. | we have recently generated a series of gamma/delta t cell clones able to kill, after in vitro immunization, an epstein-barr virus-transformed b cell line (designated e418) in a non-major histocompatibility complex-requiring fashion. a monoclonal antibody, termed anti-10h3, produced against e418 was selected by its ability to block these cytotoxic interactions. further analysis indicated that the inhibitory effects of anti-10h3 were highly selective (i.e., no blocking activity with multiple contr ... | 1991 | 1827826 |
| transformation by the oncogenic latent membrane protein correlates with its rapid turnover, membrane localization, and cytoskeletal association. | the latent membrane protein (lmp) of epstein-barr virus (ebv) has a short half-life (v. r. baichwal and b. sugden, j. virol, 61:866-875, 1987; k.p. mann and d. thorley-lawson, j. virol, 61:2100-2108, 1987), is localized in patches in the membrane (d. liebowitz, d. wang, and e, kieff, j. virol, 58:233-237, 1986), and associates with the cytoskeleton in ebv-immortalized b lymphocytes (d. liebowitz, r. kopan, e. fuchs, j. sample, and e. kieff, mol. cell. biol. 7:2299-2308, 1987; k. p. mann and d. t ... | 1991 | 1827846 |
| tissue distribution of the c3d/ebv-receptor: cd21 monoclonal antibodies reactive with a variety of epithelial cells, medullary thymocytes, and peripheral t-cells. | the cd21 antigen has been described to represent cr2, the receptor for the complement fragment c3d and also the receptor for the epstein-barr virus (ebv). monoclonal antibodies b2, hb5, and b-ly4 belong to the cd21 cluster, recognizing different epitopes of the cd21-molecule. immunohistology of lymphoid tissues employing these antibodies showed the known staining of b cells and dendritic reticulum cells. surprisingly, b2, but not hb5 or b-ly4, stained a distinct spot in the cytoplasm of a major ... | 1991 | 1830299 |
| expression of a human monoclonal anti-(rhesus d) fab fragment in escherichia coli with the use of bacteriophage lambda vectors. | a human anti-(rhesus d) antibody (igg1 lambda) fab fragment was cloned from an epstein-barr-virus-transformed cell line and expressed in escherichia coli with the use of bacteriophage lambda vectors. the cloned protein is active in binding to human erythrocytes and permits the development of a recombinant reagent for the prevention of haemolytic disease of the newborn. the method offers a rapid and effective means of rescuing human fabs from potentially unstable cell lines secreting human antibo ... | 1991 | 1830475 |
| infectious transmission of human t-cell lymphotropic virus type ii in rabbits. | to determine the susceptibility of rabbits to experimental infection with human t-cell lymphotropic virus type-ii (htlv-ii), four separate groups of four weanling rabbits each were inoculated intravenously with lethally irradiated htlv-ii-infected human cell lines mo-t (htlv-iimo-infected t cells), wil-nra (an epstein-barr virus [ebv]-transformed b-lymphoblastoid cell line infected with htlv-iinra), 729ph6neo (an ebv-transformed lymphoblastoid cell line transfected with a molecular clone of htlv ... | 1991 | 1832059 |
| human b cells cannot be triggered to kill target cells through their fc gamma rii or fc epsilon rii receptors. | there has been some controversy as to whether or not b cells can kill target cells through their fc receptors. to address this, we have examined the ability of human b cells from a variety of sources to lyse hybridoma cells with specificity for either the b cell fc gamma rii or fc epsilon rii using a reverse killing assay, as well as their ability to lyse opsonized chicken erythrocytes using a classic adcc assay. tonsil b cells, chronic lymphocytic leukemia b cells, and epstein-barr virus-induce ... | 1991 | 1832141 |
| a novel surface molecule expressed by long-term cultured t and natural killer cells is involved in cell activation. | two monoclonal antibodies (mab), termed ed6 and ld6, were obtained by immunizing mice with cytotoxic t cell lines expressing the t cell receptor (tcr) gamma/delta. these mab were selected according to their ability to trigger the cytolytic program of the immunizing cell lines in a redirected killing assay. both mab recognized molecule(s) expressed on the surface of most long-term cultured tcr gamma/delta +, tcr alpha/beta + and cd3-cd16+ lymphocytes, while it was absent on resting peripheral blo ... | 1991 | 1832383 |
| helper activity for immunoglobulin synthesis of t helper type 1 (th1) and th2 human t cell clones: the help of th1 clones is limited by their cytolytic capacity. | a large number of cd4+ human t helper type 1 (th1) clones specific for purified protein derivative and of th2 clones specific for the excretory/secretory antigen of toxocara canis, derived from the same individuals, were analyzed for both cytotoxic capacity and helper function for immunoglobulin (ig) synthesis. the great majority of th1, but only a minority of th2 clones exhibited cytolytic activity. all th2 (noncytolytic) clones induced igm, igg, iga, and ige synthesis by autologous b cells in ... | 1991 | 1833502 |
| a polymorphic fc receptor for mouse igg2b on human b cells and monocytes. | with respect to murine (m)igg1 anti-cd3 monoclonal antibody (mab), a polymorphic mitogenic response of peripheral blood mononuclear cells (pbmc) has been described which is caused by polymorphism of monocyte fc gamma rii, and which defines high responders to migg1 (migg1-hr, approximately 70% of normal individuals) and low responders (migg1-lr). pbmc also exhibit a polymorphic mitogenic response to migg2b anti-cd3 mab. in the present study 18 out of 550 individuals (3%) were migg2b-hr. purified ... | 1991 | 1838352 |
| novel chromosome translocation caused by fusion of immunoglobulin heavy and light chain v genes in a human b lymphoblastoid cell line. | the chromosome breakpoints of a translocation, t(2;14), from an epstein-barr virus-transformed human b lymphoblastoid cell line were isolated and analyzed. this unusual translocation arose as a result of the fusion of two immunoglobulin (ig) variable (v) genes, one from the heavy chain cluster on chromosome 14, the other from the light chain (k) cluster on chromosome 2. the chromosome breaks occurred within the coding sequence of each gene, and there was no obvious evidence for lymphoid v(d)j re ... | 1991 | 1840606 |
| antibody to human t-lymphotropic virus in a patient with guillain-barré syndrome (case report) | serum sample obtained from a male, 12 year old patient suffering from guillain-barré syndrome (gbs) was positive for human t-lymphotropic virus (htlv-i) antibody by the enzyme-linked immunosorbent assay (elisa) and the western blot analysis (wb). attempts to isolate enteroviruses (including poliovirus) from faecal material in both tissue culture and suckling mice were unsuccessful; in addition, acute and convalescent paired serum samples did not show any evidence of recent poliovirus infection w ... | 1991 | 1844957 |
| regulated expression of the rb "tumor suppressor gene" in normal lymphocyte mitogenesis: elevated expression in transformed leukocytes and role as a "status quo" gene. | expression of the rb retinoblastoma tumor suppressor gene product is regulated early during the stimulation of normal human peripheral blood lymphocytes, suggesting a regulatory role for the amount of this protein in mitogenesis of normal cells. when normal human peripheral blood lymphocytes were mitogenically stimulated with pokeweed mitogen, bivariate flow cytometric measurements of cellular dna and rb protein content showed an early decrease in the amount of rb protein per cell, anteceding on ... | 1991 | 1845796 |
| detection of anti-epstein-barr virus trans-activator (zebra) antibodies in sera from patients with human immunodeficiency virus. | patients with aids and aids-related complex often show symptoms of epstein-barr virus (ebv) reactivation. several ebv-encoded trans-acting factors activate the ebv lytic cycle, and one, zebra (bamhi z ebv replication activator; also called eb1), switches ebv from its latent to productive cycle. indirect immunofluorescence studies were done using human cells transfected with a recombinant dna-harboring cdna sequence spanning bzlf1 (bamhi z left frame 1) that was inserted downstream of the adenovi ... | 1991 | 1845809 |
| chronic fatigue syndrome. | | 1991 | 1845816 |
| identification of a novel ebv-induced membrane glycoprotein of 43 kda with h667 mab. | using purified b95-8 epstein-barr virus (ebv), a mab designated h667 was produced. we demonstrated by indirect membrane immunofluorescence (if) on six ebv producer cell lines and by immunoelectron microscopy that h667 reacted with a membrane antigen. h667 recognized a 43-kda ebv protein (p43) as determined by immunoblotting using purified ebv from the six producer cell lines. phosphonoacetic acid treatment of b95-8 cells was associated with the disappearance of p43, indicating that it was a late ... | 1991 | 1845828 |
| epstein-barr virus (ebv)-associated lymphoproliferative disease in the scid mouse model: implications for the pathogenesis of ebv-positive lymphomas in man. | when human peripheral blood lymphocytes (pbls) from epstein-barr virus (ebv)-seropositive donors are injected intraperitoneally into scid mice, ebv+ b cell tumors develop within weeks. a preliminary report (mosier, d. e., r. j. gulizia, s. m. baird, d. d. richman, d. b. wilson, r. i. fox, and t. j. kipps, 1989. blood. 74(suppl. 1):52a) has suggested that such tumors resemble the ebv-positive malignancy, burkitt's lymphoma. the present work shows that generally the human (hu) pbl-scid tumors are ... | 1991 | 1845872 |
| epstein-barr virus nuclear antigen 2 activates transcription of the terminal protein gene. | transcription of the terminal protein (tp) gene of epstein-barr virus (ebv) in burkitt's lymphoma cells, in ebv-negative burkitt's lymphoma cells converted with transformation-defective (p3hr1) and transformation-competent (b95-8, ag876) ebv strains, and in ebv-immortalized cell lines was studied. a tp1 cdna probe spanning the boundary between exons 1 and 2 and discriminating between tp1 and tp2 transcripts was used for s1 analysis. tp rna expression varied widely in burkitt's lymphoma cells. tp ... | 1991 | 1845900 |
| epstein-barr virus-derived plasmids replicate only once per cell cycle and are not amplified after entry into cells. | some possible ways in which replication of plasmids containing the epstein-barr virus (ebv) plasmid maintenance origin, orip, might be controlled were investigated. virtually all plasmid molecules were found to replicate no more than once per cell cycle, whether replication was observed after stable introduction of the plasmids into cells by drug selection or during the first few cell divisions after introducing the dna into cells. the presence in the cells of excess amounts of ebna1, the only v ... | 1991 | 1845903 |
| identification of cellular factors that bind specifically to the epstein-barr virus origin of dna replication. | the specific binding of hela cell factors to dna sequences at the epstein-barr virus (ebv) latent origin of dna replication was detected by gel shift experiments and dnase i footprinting analysis. these cellular proteins protected at least five discrete regions of the dna replication origin. the viral protein required for ebv plasmid replication, ebv nuclear antigen 1 (ebna-1), binds to specific sequences within the origin region. the hela cell proteins competed with ebna-1 for binding to ebv or ... | 1991 | 1845905 |
| antibody-dependent and antibody-independent complement-mediated enhancement of human immunodeficiency virus type 1 infection in a human, epstein-barr virus-transformed b-lymphocytic cell line. | a human epstein-barr virus-transformed b-cell line (ic.1) was characterized for cell surface antigen profile and permissivity to immunodeficiency virus (hiv) infection. according to cocultivation assay with mt2 cells, p24 release, and immunofluorescence assay, complement-sufficient serum enhanced in vitro infection of ic.1 cells. enhancement occurs independently of the presence of hiv type 1-specific antibodies, although more efficiently when they are present. blocking experiments with monoclona ... | 1991 | 1845908 |
| in situ hybridization for the detection of epstein-barr virus in central nervous system lymphomas. | epstein-barr virus (ebv) has been implicated in the development of lymphomas in immunocompromised patients. to test this hypothesis, 26 lymphomas involving the central nervous system (cns) (11 primary, 15 systemic) were studied for the presence of ebv. in situ hybridization (ish) was performed on formalin-fixed, paraffin-embedded tissue using a sulfur 35 (35s)-labeled ebv probe (ebv bamh1-w). the results were interpreted without knowledge of the patients' immunologic status. the ebv sequences we ... | 1991 | 1845947 |
| epstein-barr virus transformation of b lymphocytes from iga nephropathy patients and first-degree relatives results in increased immunoglobulin synthesis not restricted to iga. | in order to study b-cell activation patterns independent of t-cell regulation in patients with iga nephropathy (igan), peripheral blood mononuclear cells from 67 patients with igan, 15 first-degree relatives of patients with familial disease, and 13 normal controls were transformed with epstein-barr virus (ebv). culture supernatants of these transformed cells were assayed for levels of igg, iga, and igm, and results obtained on the three populations were compared. ebv-transformed cells of igan p ... | 1991 | 1846059 |
| single-stranded structures are present within plasmids containing the epstein-barr virus latent origin of replication. | the epstein-barr virus (ebv) latent origin of plasmid replication (orip) contains two essential regions, a family of repeats with 20 imperfect copies of a 30-bp sequence and a dyad symmetry element with four similar 30-bp repeats. each of the repeats has an internal palindromic sequence and can bind ebna 1, a protein that together with orip constitutes the only viral element necessary for ebv maintenance and replication. using single-strand-specific nucleases, we have probed plasmids containing ... | 1991 | 1846191 |
| multicomponent origin of cytomegalovirus lytic-phase dna replication. | cytomegalovirus (cmv) lytic-phase dna replication requires both trans-acting factors, such as the virus-coded dna polymerase, and a previously undefined cis-acting element, the origin, within which initiation occurs. we have located a candidate origin of cmv lytic-phase dna replication, orilyt, in both simian and human strains by assessing the ability of cloned restriction fragments to mediate phosphonoformic acid-sensitive dna replication after transfection into human fibroblasts when required ... | 1991 | 1846206 |
| chloroquine enhances replication of semliki forest virus and encephalomyocarditis virus in mice. | chloroquine (chl) has been suggested to play an important role in the development of burkitt's lymphoma by enhancing epstein-barr virus expression. herpes zoster virus incidence is markedly increased following malaria infection in children being treated with chl. recently, chl has also been shown to dramatically increase the transactivation of tat protein purified from human immunodeficiency virus. these previous studies indirectly suggest that chl may be involved in the enhancement of virus rep ... | 1991 | 1846212 |
| epstein-barr virus (ebv) glycoprotein gp350 expressed on transfected cells resistant to natural killer cell activity serves as a target antigen for ebv-specific antibody-dependent cellular cytotoxicity. | cell surface-associated viral glycoproteins are thought to play a major role as target antigens in cellular cytotoxicity and antiviral immunosurveillance. one such glycoprotein is the epstein-barr virus (ebv)-encoded glycoprotein 350 (gp350), which is expressed on both virion envelope and ebv producer cells and carries the virus attachment protein moiety. although it is known that some antibodies to gp350 can neutralize the virus, the role of this glycoprotein in ebv-specific cellular cytotoxici ... | 1991 | 1846213 |
| inhibition of 12-o-tetradecanoylphorbol-13-acetate-promoted mouse skin papilloma by saponins. | papillomas in mouse skin were initiated with 390 nmol of 7,12-dimethylbenz(a)anthracene and 1 week later, were promoted 2 times a week with 4.1 nmol of 12-o-tetradecanoylphorbol-13-acetate (tpa). five saponin-related compounds used as potential anti-inflammatory agents were applied in the same position with 82 nmol. saponin-related compounds effectively inhibited tumor formation in the sensitive mouse stock even when these compounds were given 1 h prior to tpa treatment. these results suggested ... | 1991 | 1846234 |
| nasopharyngeal carcinoma in young patients. | we have reviewed retrospectively the records of 157 patients, less than or equal to 30 years of age with nasopharyngeal carcinoma (npc) from 218 such cases identified in the tumor registry files of three major teaching hospitals in taipei, taiwan. these cases were diagnosed between 1 january 1982 and 31 december 1985, with a minimum follow-up of 2 years. the average age was 25, with a male/female ratio of 1.67. the tnm (tumor size, nodes, metastases) classification of 127 patients showed t1, 22% ... | 1991 | 1846256 |
| aids-related lymphoma. histopathology, immunophenotype, and association with epstein-barr virus as demonstrated by in situ nucleic acid hybridization. | to investigate the range of pathology shown by acquired immune deficiency syndrome (aids)-related lymphomas arising in an epidemiologically well-defined group of patients, all cases of lymphoma recognized in danish human immunodeficiency virus (hiv)-infected individuals up to the end of 1988 were studied. twenty-seven cases (26 high-grade non-hodgkin's lymphoma [nhl], 1 hodgkin's disease) were found, to give a cumulative incidence rate of 8% among danish aids patients. morphologically most nhl p ... | 1991 | 1846263 |
| detection of human papillomavirus dna in anal intraepithelial neoplasia and anal cancer. | forty anal paraffin-embedded tissue specimens from 24 subjects were studied for the presence of human papillomavirus (hpv) types 6, 11, 16, 18, 31, and 33, herpes simplex virus (hsv), epstein-barr virus, and cytomegalovirus dna by using the polymerase chain reaction. these tissues ranged from histologically normal to invasive squamous cell carcinoma. hpv dna was detected in the invasive anal cancer tissues of 11 of 13 subjects. hpv types were segregated by histopathological severity, with hpv 16 ... | 1991 | 1846314 |
| composite response of naive t cells to stimulation with the autologous lymphoblastoid cell line is mediated by cd4 cytotoxic t cell clones and includes an epstein-barr virus-specific component. | we have approached the challenge of generating a primary t cell response to epstein-barr virus (ebv) in vitro by stimulating naive t cells with the autologous ebv-transformed lymphoblastoid cell line (lcl), a rich source of ebv-associated cytotoxic t lymphocyte (ctl) epitopes. responsive t cells from three ebv-seronegative donors were cloned in agarose, phenotyped for t cell markers by flow cytometry, and their cytotoxic properties analyzed in the 51cr release assay. most clones (greater than 95 ... | 1991 | 1846323 |
| defective control of epstein-barr virus-infected b cell growth in patients with x-linked lymphoproliferative disease. | we studied the cellular function and lymphokine production of t cells from patients with x-linked lymphoproliferative disease (xlp) when activated by the challenge with epstein-barr virus (ebv) infection. we used an assay system in which t cells were stimulated with membrane antigens of autologous ebv-infected b lymphoblastoid cell lines (b-lcl) and we examined cellular and humoral factors derived from the stimulated t cells which control the growth of ebv-infected b-lcl. immunoglobulin secretio ... | 1991 | 1846327 |
| expression of epstein-barr virus-encoded growth-transformation-associated proteins in lymphoproliferations of bone-marrow transplant recipients. | the expression of epstein-barr virus (ebv)-encoded, growth-transformation-associated proteins was studied in lymphoproliferations of 9 allogeneic bone-marrow transplant (bmt) recipients. immunoblots of cell lysates were probed with polyspecific and monospecific antisera directed against ebna 1, 2, 3 and 6, and the membrane protein lmp. all tumors expressed ebna 1 and lmp. ebna 2 was detected in the tumors of 8 patients, and ebna 3 and 6 in the tumors of 5 patients. the lmp regulatory sequences, ... | 1991 | 1846349 |
| expression of vhiii-associated cross-reactive idiotype on human b lymphocytes. association with staphylococcal protein a binding and staphylococcus aureus cowan i stimulation. | it has been demonstrated that staphylococcal protein a (spa) has an "alternative" binding site with specificity for human ig h chain v region of the vhiii subgroup. because the major mitogenic component of staphylococcus aureus cowan i (sac) is spa, it is possible that sac stimulates a subpopulation of b cells expressing ig of the vhiii h chain subgroup. in the present study, we have investigated further the relationship between spa binding and the expression of vhi- or vhiii-associated cross-re ... | 1991 | 1846385 |
| is sequential use of alg and okt3 in renal transplants associated with an increased incidence of fulminant posttransplant lymphoproliferative disorder? | | 1991 | 1846454 |
| relationship between okt3 administration, ebv seroconversion, and the lymphoproliferative syndrome in pediatric liver transplant recipients. | | 1991 | 1846468 |
| the spectrum of epstein-barr virus infection with hepatitis following liver transplantation. | | 1991 | 1846474 |
| opportunistic viral hepatitis in liver transplant recipients. | | 1991 | 1846476 |
| novel downstream elements upregulate transcription initiated from an epstein-barr virus latent promoter. | the modulation of nuclear antigen expression from the epstein-barr virus (ebv) genome in virus-infected cells is crucial to the establishment and maintenance of the immortalized state. the ebv nuclear antigen (ebna) genes are all transcribed from either of two promoters whose alternate usage is not understood. we have studied the ebna transcriptional domain by using various promoter constructs to transfect a variety of ebv positive cell lines and then measured promoter activity by rnase protecti ... | 1991 | 1846596 |
| infectious mononucleosis, epstein-barr virus, and chronic fatigue syndrome: a prospective case series. | epstein-barr viral infection, specifically infectious mononucleosis, typically has a more protracted course than other acute viral illnesses. some recent observers have additionally suggested the possibility that epstein-barr virus (ebv) is the etiologic infectious agent in chronic fatigue syndrome, based on the finding of higher proportions of elevated antibodies to the ebv early antigen in some patients complaining of chronic fatigue. straus et al reported on 23 patients with chronic fatigue, ... | 1991 | 1846641 |
| eap, a highly conserved cellular protein associated with epstein-barr virus small rnas (ebers). | human b lymphocytes latently infected with epstein-barr virus (ebv) synthesize very large amounts (5 x 10(6)/cell) of two small nuclear rnas called ebers (epstein-barr encoded rnas). these rnas are of unknown function and, like many rna polymerase iii (pol iii) transcripts, bind the la autoantigen. we have discovered that the ebers also associate with a second highly abundant host-encoded protein designated eap (eber associated protein). human eap is a small (14,777 dalton, 128 amino acid) polyp ... | 1991 | 1846807 |
| inhibition of tumor necrosis factor-alpha transcription by epstein-barr virus. | tumor necrosis factor-alpha (tnf-alpha), which is produced mainly by monocyte/macrophage cells, has diverse physiological functions on lymphoid cells. moreover, it has been shown that tnf-alpha exhibits antiviral activities. here we report that epstein-barr virus (ebv), a b lymphotropic human herpes virus that interacts intimately with the immune system, exerts a strong inhibitory effect on tnf-alpha production by lipopolysaccharide-treated peripheral blood leukocytes as well as by monocytic cel ... | 1991 | 1846816 |
| isotype commitment of human b cells that are transformed by epstein-barr virus. | epstein-barr virus (ebv) can transform a subpopulation of preactivated b cells thus promoting their growth and differentiation into plasma cells. in ebv-transformed clones of igm-producing cells, the heavy chain constant region (ch) genes on the productive allele are fixed in germ-line configuration, whereas in isotype-switched clones the ch genes proximal to the expressed ch gene are deleted. in order to define more precisely the ebv-susceptible b cells, we sorted subpopulations of b cells on t ... | 1991 | 1846818 |
| cyclosporin a renders target cells resistant to immune cytolysis. | exposure of cytolytically susceptible human target cells with therapeutic concentrations of the immunosuppressive drug cyclosporin a renders these cells highly resistant to t cell-mediated, natural killer (nk) cell-mediated, and complement-mediated cytolysis. the resistance is dose dependent, time dependent and reversible. the resistance is accompanied by target cell growth inhibition as measured by thymidine uptake. surprisingly, target cell growth inhibition induced by serum depletion is assoc ... | 1991 | 1846819 |
| ig repertoire of human polyspecific antibodies and b cell ontogeny. | a total of 463 ebv ig-secreting clones were derived from embryonic tissues, cord blood, and adult peripheral blood. subcloning and analysis of the h and k loci (germline vs rearranged dna status) of 44 primary clones insured clonality in at least 92% of cases. whatever the cell origin, a somewhat constant proportion of clones (i.e., 11 to 16%) expressed polyspecific antibodies when tested on a panel of nine ag, including self-ag. the vh and vk repertoires have been studied using vh1-vh6 and vk1- ... | 1991 | 1846900 |
| detection of herpesvirus dna in vitreous and aqueous specimens by the polymerase chain reaction. | members of the herpesvirus family, cytomegalovirus (cmv), epstein-barr virus (ebv), and herpes simplex virus (hsv), have been recognized as causal agents of chorioretinal inflammatory diseases. we investigated the use of the polymerase chain reaction for the detection of cmv, hsv, and ebv genomes in aqueous, subretinal fluid, and vitreous specimens in patients with clinically diagnosed cmv retinitis. cytomegalovirus but not hsv or ebv genomic sequences were detected in all of these clinical spec ... | 1991 | 1847043 |
| aggressive peripheral t-cell lymphomas containing epstein-barr viral dna: a clinicopathologic and molecular analysis. | the epstein-barr virus (ebv) has been shown to be associated with posttransplant lymphoma, hodgkin's disease, and t-cell lymphoma, in addition to african burkitt's lymphoma. in a retrospective study of 56 consecutive cases of t-cell lymphoma, ebv dna was found by southern blot and in situ dna hybridization in 10 (20%) of 50 peripheral t-cell lymphomas, but in none of six cases of t-lymphoblastic lymphoma. peripheral t-cell lymphomas containing ebv dna could be subclassified into three categories ... | 1991 | 1847084 |
| methods of detection of new families with x-linked lymphoproliferative disease. | during the past decade, 240 males with x-linked lymphoproliferative disease (xlp) within 59 unrelated kindreds have been identified worldwide. one half of the patients have developed fatal infections mononucleosis, about one third have acquired hypogammaglobulinemia, and another one fourth have developed malignant lymphoma. less commonly occurring phenotypes include hyperimmunoglobulinemia m, bone marrow hypoplasia, and necrotizing lymphoid vasculitis. the fatal infectious mononucleosis phenotyp ... | 1991 | 1847089 |
| extra y chromosome in chronic lymphoproliferative disorders. | using separated lymphocytes from 95 male patients with b-cell lymphoproliferative disorders, we have established both epstein-barr virus (ebv)-transformed lymphoblastoid cell lines and short-term cultures with polyclonal b-cell mitogens. cytogenetic studies of these patients revealed an extra y chromosome in 4 of 71 male cell lines examined. an extra y chromosome appeared to be the sole karyotype change (47,xy, + y) in 2 of these 4 patients. the extra y chromosome was accompanied by extra copies ... | 1991 | 1847090 |
| generation and cloning of stable human ige-secreting cells that have rearranged the c epsilon gene. | although the secretion of ig isotypes other than igm is generally accompanied by a dna rearrangement that deletes c mu (and the other igch genes located between vdj and the expressed ch gene), a system has recently been described that generates a high frequency of ige-secreting cells that have failed to delete igch genes or to rearrange their c epsilon genes. these cells, derived from ebv-transformed human pbmc, secrete igm and igd as well as ige. to determine whether the absence of c epsilon re ... | 1991 | 1847163 |
| platelet-activating factor induces phospholipid turnover, calcium flux, arachidonic acid liberation, eicosanoid generation, and oncogene expression in a human b cell line. | platelet-activating factor is a potent mediator of the inflammatory response. studies of the actions of platelet-activating factor have centered mainly around neutrophils, monocytes, and platelets. in this report we begin to uncover the influence of platelet-activating factor on b lymphocytes. employing the ebv-transformed human b cell line skw6.4, we demonstrate that platelet-activating factor significantly alters membrane phospholipid metabolism indicated by the incorporation of 32p into phosp ... | 1991 | 1847170 |
| immunological studies on the epstein-barr virus encoded alkaline deoxyribonuclease found in virus-producing lymphoblastoid cells. | antisera were raised against a purified recombinant form of the epstein-barr virus (ebv) alkaline deoxyribonuclease (dnase) expressed in escherichia coli. these sera were shown to be reactive with lymphoblastoid cells expressing ebv antigens (b95-8, p3hr-1 and raji). immunostaining studies of cells expressing ebv antigens revealed that the dnase was a component of the restricted early antigen complex of ebv. western blot analysis of these chemically induced cells revealed that the polypeptide as ... | 1991 | 1847177 |
| oral hairy leukoplakia in hiv infection: a diagnostic pitfall. | twenty-nine human immunodeficiency virus (hiv)-infected patients with white, nonremovable lesions on the lateral border of the tongue, clinically suggestive of oral hairy leukoplakia (hl), were studied. in particular, the value of local antifungal therapy in establishing the diagnosis of hl was investigated. in 15 patients (52%) the lesions could be ultimately attributed to a candidal infection of the tongue. in 10 of the remaining 14 patients, a biopsy was obtained from lesions persisting after ... | 1991 | 1847236 |
| the bi'lf4 trans-activator of epstein-barr virus is modulated by type and differentiation of the host cell. | we have analyzed the activity and regulated expression of a new epstein-barr virus (ebv) trans-activator (i'ta) encoded by left reading frame 4 (bi'lf4) of the bamhi i'fragment. the gene was detected in all genomes of established ebv strains and individual isolates, with the exception of b95-8, where the type-specific deletion of this open reading frame is tolerated in vitro. specific trans-activation of two ebv promoters (early ms and i'ta promoter) could be shown in cotransfection assays. the ... | 1991 | 1847255 |
| high-level expression of the epstein-barr virus alkaline deoxyribonuclease using a recombinant baculovirus: application to the diagnosis of nasopharyngeal carcinoma. | the epstein-barr virus (ebv) alkaline deoxyribonuclease (dnase) was inserted into the baculovirus autographa californica nuclear polyhedrosis virus (acmnpv). infection of the insect cell line spodoptera frugiperda (sf9) with the recombinant virus led to the expression of an enzymatically active alkaline dnase. the recombinant ebv alkaline dnase was highly soluble, and the recombinant baculovirus produced approximately 10-20 mg of ebv dnase per 1 x 10(9) cells. the recombinant enzyme activity was ... | 1991 | 1847261 |
| epstein-barr virus infection precedes clonal expansion in burkitt's and acquired immunodeficiency syndrome-associated lymphoma. | the epstein-barr virus (ebv) is associated with distinct forms of human lymphoid malignancies, including the endemic (ebl) and sporadic forms of burkitt's lymphoma (sbl) and acquired immunodeficiency syndrome-associated non-hodgkin lymphoma (aids-nhl). however, whether ebv has a pathogenetic role in these tumors or is a passenger virus has not been conclusively demonstrated. one element to distinguish between these two possibilities is to determine whether ebv infection has preceded and, thus, p ... | 1991 | 1847310 |
| treatment of end-stage chronic obstructive pulmonary disease with double lung transplantation. | six patients with end-stage emphysema (age 44 +/- 2 years) underwent double lung transplantation (tx) from june 1988 through may 1990. all suffered from severe inanition and required oxygen therapy. the ischemic time was 193 +/- 28 minutes. post-tx immune suppression was okt3 (14 days), cyclosporine (trough levels of 150 +/- 25 ng/ml), azathioprine to keep wbc at 3,000 to 5,000/cu mm (1 to 3.0 mg/kg/day) and following okt3, a tapering prednisone regimen. two rejection episodes that occurred in t ... | 1991 | 1847323 |
| scid mouse model of epstein-barr virus-induced lymphomagenesis of immunodeficient humans. | immunodeficient humans are at very high risk of developing epstein-barr virus (ebv)-induced lymphomagenesis. severe combined immunodeficient mice (scid) have been shown to develop lymphoproliferative disease (lpd) following transfer of peripheral blood leukocytes (pbl) with latent ebv. to study mechanisms of lymphomagenesis, we compared results of engraftment of pbl from normal donors and immunodeficient donors with x-linked lymphoproliferative disease (xlp). graft-versus-host disease (gvhd) dev ... | 1991 | 1847355 |
| when epstein-barr virus persistently infects b-cell lines, it frequently integrates. | in this study we used gardella gel analysis of intact dna, southern blotting of digested dna, and fluorescence in situ hybridization to provide complementary and unequivocal information on the state of the epstein-barr virus (ebv) genome in persistently infected cells. the fluorescence in situ hybridization technique allowed us to directly visualize both integrated and episomal ebv dna at the single-cell level. we show here that circularization of the ebv genome is rarely detected upon infecting ... | 1991 | 1847452 |