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thoughts on kawasaki disease etiology. 1979214591
polyclonal immunoglobulin secretion by human b lymphocytes exposed to epstein-barr virus in vitro. 1979221572
lymphocyte activation by the tumor-promoting agent 12-o-tetradecanoylphorbol-13-acetate (tpa).tpa, a highly active tumor-promoting agent, is an effective mitogen for primate peripheral blood lymphocytes. optimal stimulation of human lymphocytes was obtained 4 days after the addition of tpa at a concentration of 7.5 ng/ml. lymphocyte fractionation experiments demonstrated that both t and b cells incorporated 3h-thymidine significantly in response to tpa. lymphocyte blastogenesis was not due to the reactivation of latent herpesviruses by the tumor promoter, since similar responses to tpa w ...1979221578
the effects of mitogens on the expression of epstein-barr virus antigens in human lymphoid cell lines.treatment of human lymphoblastoid cells with either phytohemagglutinin (pha), concanavalin a, staphylococcus protein a, or polyinosinic acid-polycytidylic acid, in combination with 5-iodo-2' deoxyuridine (iudr) markedly increased the expression of epstein-barr virus (ebv) early antigen (ea) relative to iudr alone. such treatment did not, however, modify the production of virus capsid antigen in any of the lymphoid cell lines tested. the effect of pha on ea induction in raji cells was not accompa ...1979221589
cross-neutralization of infectious mononucleosis and burkitt lymphoma strains of epstein-barr virus with hyperimmune rabbit antisera. 1979221591
incidence of heterophil antibody responses in children with infectious mononucleosis. 1979221629
the epstein-barr virus and eb virus infections in childhood. 1979221638
epstein-barr virus-related antibody patterns in ataxia-telangiectasia.epstein-barr virus-related antibody titres were determined in twenty-seven patients with ataxia-telangiectasia (at) and twenty-two healthy members of their families, in twenty-two patients with other diseases, among them ten with behçet's disease and ten with various primary immune deficiencies, and fifteen healthy members of their families, and in twenty-three unrelated healthy controls. the at patients showed an increased incidence (55.6%) of high antibody titres (greater than or equal to 1:32 ...1979219976
tumour promoter tpa enhances transformation of human leukocytes by epstein-barr virus. 1979221841
cell surface glycoprotein changes in epstein-barr virus-positive and -negative human hematopoietic cell lines.it has previously been shown that differential fucose labelling of many normal and homologous tumor cells, followed by proteolytic release and degradation, yields glycopeptides which upon gel filtration shown an increase in fast-eluting glycopeptides for the tumor cells. this technique has now been applied to cell-surface glycoproteins of different human hematopoietic cell lines. these lines included epstein-barr virus (ebv)-carrying lymphoblastoid cell lines of presumed non-neoplastic origin, a ...1979220199
detection of a nuclear antigen in herpesvirus ateles-carrying marmoset lines by the acid-fixed nuclear binding technique.in vitro binding of a herpesvirus ateles (hva)-associated soluble antigen to amphibian erythrocyte nuclei was demonstrated by the acid-fixed nuclear binding technique in combination with anticomplement immunofluorescence. incubation of concentrated salt-extracted soluble antigens derived from hva-carrying marmoset lines with methanol/acetic acid-fixed erythrocytes of frogs and salamanders resulted in a brilliant nuclear fluorescence after exposure to a live virus-boostered, anti-hvs-positive squ ...1979221913
establishment and characterization of a human null-cell lymphoblastic lymphoma cell line (k-ll-3).a new cell line (k-ll-3) with exceptional characteristics was derived from a bone-marrow aspirate from a patient with poorly differentiated lymphoblastic lymphoma. the cell line was established and the cells maintained on agar feeders for over 1 year. the morphology and cytochemical staining of the primary tumor and the cell line were remarkably similar. the growth characteristics, chromosome pattern, cell-surface receptors and epstein-barr pattern, cell-surface receptors and epstein-barr virus ...1979220200
[search for unknown viruses of human neoplasms]. 1979220344
[cytomegalovirus and eb-virus antibodies in patients with non-a non-b hepatitis (author's transl)]. 1979219957
[125i] clq-binding activity and its relationship with anti-epstein--barr virus antibodies in sera from nasopharyngeal carcinoma patients. 1979220051
superinfection of ebv-carrying lines with herpes virus papio (hvp): induction of early antigen (ea) and inhibition of cellular dna synthesis. 1979220052
size of the intracellular circular epstein-barr virus dna molecules in infectious mononucleosis-derived human lymphoid cell lines.the size of non-integrated circular epstein-barr virus (ebv) dna molecules isolated from seven different human lymphoblastoid cell lines of infectious mononucleosis origin has been determined by sedimentation analysis and by direct contour length measurements on electron micrographs. six lines had intracellular circular ebv genomes of the same size as linear virion dna molecules. the seventh line, established with the b95-8 strain of ebv, was the only one found to have circular ebv dna molecules ...1979219261
retinoic acid inhibition of epstein-barr virus induction. 1979219367
epstein-barr virus antibody responses and clinical illness in renal transplant recipients.among 88 renal transplant recipients evaluated for a change in epstein-barr virus (ebv) antibody status in the period after transplant, 22 showed a 4-fold rise and eight showed an 8-fold or greater rise in ebv antibody. among the patients with an 8-fold or greater ebv antibody rise, the occurrence of fever was frequent, one patient developed a lymphoproliferative reaction, and one died with a malignant ebv infection. patients without pretransplant antibody showed a longer mean time to antibody r ...1979219556
viral serology (hepatitis b virus, cytomegalovirus, epstein-barr virus) and abnormal liver function tests in transfused patients with hereditary hemorrhagic diseases. 1979219564
characterization of cross-reacting antigens on the epstein-barr virus envelope and plasma membranes of producer cells.a rabbit antiserum has been prepared against the b95-8 transforming strain of ebv. the antiserum has a high virus neutralizing titer (approximately 1:1000) against both the marmoset b95-8 ebv and the human p3hr-1 ebv. the neutralizing antibodies may be absorbed completely with ebv producer cell lines, but not with nonproducer cell lines or producer cell lines treated with phosphonoacetic acid (paa) so as to be nonproducer. after repeated absorption with paa-treated b95-8, the serum remains react ...1979217544
susceptibility of lymphoblastoid cells to infection with human cytomegalovirus.human lymphoblastoid cells of b- and t-cell origin were examined for their in vitro susceptibility to infection with human cytomegalovirus (cmv). results of infectious-center assays, at virus to cell ratios of 10, indicated that in each of the lymphoblastoid cell lines tested less than 1% of the cells produced infectious virus. under these conditions, cmv specific antigens were undetectable. infection of lymphoblastoid cells with cmv resulted in atypical virus growth curves similar to those obta ...1979217829
tumor initiators and promoters in the induction of epstein-barr virus.the effect of various tumor initiators and promoters on induction of persisting epstein-barr virus (ebv) in different lines of lymphoblastoid cells was analyzed. neither five polycyclic aromatic hydrocarbons, amongst them potent tumor initiators (e.g., 7,12-dimethylbenz[a]anthracene), nor the potent (ultimate) liver carcinogen n-acetoxy-n-2-acetylamino-fluorene induced ebv. a series of compounds, representing three classes of tumor-promoting diterpene esters (e.g., 12-o-tetradecanoylphorbol-13-a ...1979218219
cytomegalovirus granulomatous hepatitis.three patients with granulomatous hepatitis due to cytomegalovirus are described. they are compared to the three previously described patients with this disease, and their clinical and serologic characteristics are discussed. similarities and differences between infectious mononucleosis (epstein-barr virus) and cytomegalovirus infections are adduced. that cytomegalovirus may be a cause of granulomatous hepatitis in the adult is stressed.1979218449
simultaneous infection with multiple herpesviruses. 1979218450
[antiviral activity of interferon and its inducers in human lymphoblastoid and somatic cells].the antiviral effect of interferon inductors, such as poly-i--poly-c, phage f2 rna replicative form and low molecular inductor gsn and their influence on cellular dna synthesis were studied in the cultures of lymphoblastoid (inplanting lines raji namalva) and somatic human cells. the semliki forest virus used as the test organism multiplicated well in cells raji accumulating up to 9 lg bou/ml. the two-strand rna was less active in the lymphoid cells than in the somatic ones. gsn was 10 times mor ...1979220908
effects of nucleoside analogs on epstein-barr virus-induced transformation of human umbilical cord leukocytes and epstein-barr virus expression in transformed cells.two methods of assay, measuring (i) stimulation of host cell dna synthesis by [(3)h]thymidine incorporation and (ii) morphological transformation in microtiter plates, were employed to determine what effect treatment during infection with adenine arabinoside or 5-iodo-5'-amino-2',5'-dideoxyuridine has on epstein-barr virus (ebv) (b95-8)-induced transformation of human umbilical cord leukocytes. it was found that adenine arabinoside inhibited ebv-induced transformation in a dose-dependent manner ...1979218497
epstein-barr virus-specific rna. iii. mapping of dna encoding viral rna in restringent infection.namalwa and raji cells, originally obtained from a burkitt tumor biopsy, grow as continuous cell lines in vitro and contain the epstein-barr virus (ebv)-related nuclear antigen ebna (b. m. reedman and g. klein, int. j. cancer 11:499-520, 1973) and rna homologous to at least 17 and 30% of the ebv genome, respectively (s. d. hayward and e. kieff, j. virol. 18:518-525, 1976; t. orellana and e. kieff, j. virol. 22:321-330, 1977). the polyribosomal and polyadenylated [poly(a)+] rna fractions of namal ...1979219221
in vitro growth of cutaneous t-cell lymphomas.it is relatively easy to propagate eb virus-transformed normal and malignant b lymphocytes in vitro. normal t lymphocytes divide for short periods of time after exposure to many mitogens. exposure of normal t lymphocytes to lymphocyte-conditioned medium (lcm) permits them to divide for longer periods of time, but permanent cell lines cannot be established. intial attempts to grow malignant t cells from patients with cutaneous t-cell lymphomas (ctcl) in unsupplemented growth medium resulted in th ...1979221120
nasopharyngeal carcinoma in an alaskan eskimo family: report of three cases.nasopharyngeal carcinoma (npc) has been reported to occur in alsaka natives (eskimos, indians, and aleuts) at a rate greater than 15 times the rate for u.s. whites. at least 3 cases of npc have occurred in close relatives of a northern alaskan eskimo family. all 3 patients had poorly differentiated tumors and have died. blood groups, hla antigens, and antibodies to epstein-barr virus (ebv) were studied in the family members. ebv titers were elevated in the 1 npc patient tested and in 1 of 15 oth ...1979220446
extraction of soluble antigens of epstein-barr virus, herpesvirus salmirl, and herpesvirus ateles with the use of glycine.for extraction of soluble antigen from cells infected with epstein-barr virus, herpesvirus salmirl, and h. ateles, 0.1 m glycine (ph 9.5) was used. this method yielded increased amounts of the antigen containing much less cell debris. lymphoblastoid cells infected with epstein-barr virus could maintain up to 50% viability after the extraction procedure. these cells could be used again after an appropriate interval in culture. the usefulness of this technique is discussed.1979220447
infection with epstein-barr virus. 1979217475
genetic mechanisms in cancer predisposition: report of a cancer family.a family is described in which four children developed cancer affecting different organs:lymphoma, meningeal sarcoma, osteogenic sarcoma, and adenocarcinoma of the cecum. since there was only one other case of cancer in previous generations of this family, an hypothesis is put forth to explain this unusual aggregation on the basis of recombination of common genes. it is postulated that each parent carried a different combination of genes which, though not associated with increased cancer predisp ...1979217526
induction of the epstein-barr virus (ebv) cycle in latently infected cells by n-butyrate. 1979220786
a possible function of epstein-barr virus-determined nuclear antigen (ebna): stimulation of chromatin template activity in vitro. 1979220793
infectious mononucleosis and epstein-barr virus. 1. epidemiology, pathogenesis, immune response.the evidence linking epstein-barr virus causally with infectious mononucleosis is compelling. the disease occurs only in persons who lack antibody to this virus, and serologic findings during the acute illness provide almost certain evidence for its etiologic association. additional evidence is derived from transformation studies using lymphocytes from persons infected with epstein-barr virus. epidemiologic studies have related the need for close contact with salivary secretions to the low commu ...1979215976
infectious mononucleosis and epstein-barr virus. 2. clinical picture, diagnosis, management.the diagnosis of infectious mononucleosis is confirmed by hematologic, biochemical, and serologic data. an absolute increase in peripheral mononuclear cells to at least 4,500/cu mm is an essential feature. the serologic hallmark of the disease is the presence of heterophile antibody. the age of the patient may affect the clinical presentation and laboratory findings. therapy is largely supportive, with steroids being used only in the presence of certain severe complications.1979215977
[blood transfusion and viral diseases. recent acquisitions concerning viral hepatitis viruses, cytomegaloviruses and epstein-barr virus].in recent years, an increasingly clear picture has been formed of the virus-induced syndromes that may follow a blood transfusion or the use of blood derivatives. up to about 10 years ago, post-infusion infection was predominantly due to serum hepatitis. blumberg's discovery of hbsag (formerly known as australia antigen) has made it possible to check and prevent viral hepatitis, type b, and to recognise such distinct forms as the mononucleosis-like syndrome caused by cytomegalic virus, infectiou ...1979219399
burkitt's lymphoma in iraq. clinical and pathological study of forty-seven patients.the clinical and pathological features of 47 patients with burkitt's lymphoma seen at the university of baghdad teaching hospital over the years 1969-1977 are described. there were 30 males and 17 females with a mean age of 7.5 years. all patients presented with advanced disease; 44 were in stage iii and 3 in stage iv, (1 with central nervous system involvement; 1 with bone-marrow involvement; 1 presented as acute leukaemia). the abdomen, with or without other sites, was involved in 42 patients. ...1979215559
14q+ marker chromosome in an ebv-genome-negative lymph node without signs of malignancy in a patient with ebv-genome-positive nasopharyngeal carcinoma.in a patient with an ebv-genome-positive nasopharyngeal carcinoma, an ebv-genome-negative inguinal lymph node without histological evidence of malignant lymphoma or metastatic carcinoma growth was found to contain a 14q+ marker chromosome, identified as an 8;14 translocation, in all cells analyzed. this observation indicates that chromosome aberrations may precede histological signs of malignancy. the possible implication of this finding in relation to the postulated role of the 14q+ marker and ...1979215561
correlated expression of a b-lymphocyte-specific glycoprotein (gp27/35) and the ebv receptor/c3 receptor complex in sublines from the same burkitt lymphoma.a series of virus-producer and non-producer sublines, derived from the burkitt lymphoma line jijoye and its p3hr-1 clone, were previously found to differ in the expression of ebv receptors and, in parallel, c3 receptors. the differences could be related to an "internal selection" caused by the cytopathic p3hr-1 virus variant, favouring the growth of receptor-negative cells. we have now analyzed the same lines for the expression of a b-lymphocyte-specific glycoprotein (gp27/35). a close parallell ...1979215562
nucleosomal structure of epstein-barr virus dna in transformed cell lines.micrococcal nuclease digestion was used to analyze epstein-barr virus (ebv) dna structure in nuclei of transformed cells. digests of virus-producing (p3hr-1), non-virus-producing (raji), and superinfected rajii cell nuclei were fractionated by electrophoresis on agarose gels, transferred to nitrocellulose, and hybridized to 32p-labeled ebv dna. the viral dna of raji nuclei produced a series of bands on electrophoresis whose lengths were integral multiples of a unit size, which was the same as th ...1979219253
establishment of a malignant, epstein-barr-virus (ebv)-negative cell-line from the pleura effusion of a patient with hodgkin's disease. 1979216443
[clinical significance of sensitive and specific variations of the paul-bunnell reaction]. 1979218156
cell-density-dependence for growth in agarose of two human lymphoma lines and its decrease after epstein-barr virus conversion.we have compared the growth in agarose medium of two ebv-negative burkitt lymphoma lines, bjab and ramos, and that of their ebv-converted derivatives. optimal conditions for growth in agarose medium are described. the original ebv-negative lines can grow in agarose to a high efficiency, provided a critical level of cell concentration is attained. below this level, there is no growth at all. ebv-converted sublines form colonies at a much lower cell concentration, and their maximal plating efficie ...1979215564
biochemical identification of primate lymphoid cell-surface glycoproteins.we have employed the galactose oxidase-tritiated sodium borohydride labelling method to examine the surface glycoproteins of cotton-topped marmoset and other primate cell lines either established from tumors or transformed in vitro by different lymphotropic herpesviruses. the labelled surface glycoproteins were separated on acrylamide gels in the presence of sodium dodecyl sulfate (sds) and analyzed by fluorography. our results indicate that (1) lymphocytes of the same class from different prima ...1979215565
hla-d locus typing in ankylosing spondylitis and reiter's syndrome.hla-d typing of 44 patients with ankylosing spondylitis (as) and 31 patients with reiter's syndrome (rs) did not show increased frequency of any particular dw allele in either population of patients as compared to controls. such studies also allowed each patient's general response to be compared with other general responses within each experiment. contrary to reports of diminished lymphocyte responses in as patients, hyperresponsiveness in both as and rs patients was found.1979218595
request for specimens from organ transplant and immunodeficiency patients who developed lymphomas. 1979218723
analysis of the transformation of human lymphocytes by epstein-barr virus. i. sequential occurrence from the virus-determined nuclear antigen synthesis, to blastogenesis, to dna synthesis.a b-cell population of human cord blood lymphocytes was exposed to the b95-8 strain of epstein-barr virus (ebv), and simultaneous observations of immunofluorescence, cellular morphology and autoradiography were carried out in each individual cell. it was evident that ebv-determined nuclear antigen (ebna) synthesis occurred prior to blastogenic response and dna synthesis. ebna-positive cells could be observed as early as 12 h after infection and reached a maximum of 17% at 24 h, followed by a pla ...1979218892
replication of herpes simplex virus in human b lymphocytes stimulated by epstein-barr virus.human b lymphocytes prepared from adult blood or cord blood by the use of neuraminidase-treated sheep red blood cells which did not respond mitogenically to phytohemagglutinin or pokeweed mitogen, responded well to the b95-8 strain of epstein-barr virus, but not to the p3hr-1 strain, with increased incorporation of tritiated thymidine. replication of herpes simplex virus could be demonstrated in cultures of b lymphocytes preincubated with the b95-8 strain.1979218893
sensitive solid-phase radioimmunoassay for detection of human immunoglobulin g antibodies to varicella-zoster virus.a sensitive solid-phase radioimmunoassay for detection of antibodies to varicella-zoster virus (vzv) is described. the antigen consisted of a sonically disrupted extract of vzv-infected human embryo cells. 125i-labeled rabbit anti-human immunoglobulin g (igg) specific for the fc portion of human igg was used to detect human igg bound to viral antigen. with this technique, 193 human sera were evaluated for their igg antibody titer against zvz. subjects included 62 healthy adults, 33 young childre ...1979219015
characterization of an epstein-barr virus-induced dna polymerase.the addition of iododeoxyuridine to p3hr-i cell cultures led to a large increase in both epstein-barr virus (ebv)-induced dna polymerase activity and early antigen-positive cells. this ebv-induced dna polymerase was separated from the cellular alpha- and beta-polymerases by sequential column chromatography on sepharose 6b, deae-cellulose, and phosphocellulose, resulting in partial purification of about 320-fold. the partially purified-ebv dna polymerase could be distinguished from the cellular d ...1979219209
differential effect of phosphonoacetic acid on early antigen synthesis in two epstein-barr virus producer cell lines. 1979217163
enhanced induction of growth of b lymphoblasts from fresh human blood by primate type-c retroviruses (gibbon ape leukemia virus and simian sarcoma virus). 1979216638
a comparison of the prognostic value of antibody-dependent lymphocyte cytotoxicity and other ebv antibody assays in chinese patients with nasopharyngeal carcinoma.antibody titres to ebv-associated antigens in chinese npc patients were analysed according to length of survival after diagnosis and to disease stage. geometric mean titres of adlc antibody were highest in the long-term survivors, whereas vca, ea and ebna antibody titres showed an inverse relationship to survival. high vca, ea and ebna titres were less frequent, and high adlc titres were more frequent in long-term survivors than in intermediate or short-term survivors. the association of geometr ...1979216639
antibody responses to herpesvirus papio antigens in baboons with lymphoma.an epstein-barr virus-related herpesvirus, termed herpesvirus papio (hvp), was isolated from baboons (papio hamadryas) at the institute of experimental pathology and therapy, sukhumi, ussr, where there is a continuing outbreak of lymphoma. in the present study sera from diseased baboons and from age- and sex-matched control animals were examined for antibodies to hvp antigens. results showed that animals with lymphoid disease had antibodies to hvp virus capsid, early, soluble, and nuclear antige ...1979216640
differences in ebv receptor concentration between in vitro ebv-converted lymphoma sublines reflect biological differences between the converting viral substrains.we have compared the ebv-receptor concentration of two originally ebv-negative human b-cell lymphoma lines, after in vitro conversion with the transforming b95-8 or the cytopathic p3hr-1 eb-viral substrain, respectively, into permanent ebv-carrying sublines. receptors were measured by the quantitative ebv-absorption bioassay of sairenji and hinuma (1973). ebv receptor concentration of all p3hr-1 virus-converted sublines was significantly reduced, in comparison with the b95-8 virus-converted subl ...1979216641
detection of antibody to a new antigen induced by epstein-barr virus in rheumatoid arthritis.a new antigen which is different from epstein-barr virion antigen was detected in nc-37 cells infected with epstein-barr virus (ebv). a significant elevation of the titer of antibody to this new antigen was observed in the sera of the patients with rheumatoid arthritis (ra), but not in the sera of controls. from the standpoint of the etiologic role of ebv in ra, it is interesting that the antibody to the new antigen is not detected in the healthy persons in contrast to other viral antibodies. th ...1979216133
interaction of factors associated with cancer of the nasopharynx.a retrospective study of nasopharyngeal carcinoma (npc) revealed that smoking, working under poor ventilation, use of nasal balms or oil for nasal and throat troubles, use of herbal drugs, and anti-ebv antibody titer were found statistically associated. the dural interactions of these factors to the risk of npc were presented. except in work conditions with poor ventilation and when herbal drugs are used, all the combinations were synergistic. the synergistic actions were especially remarkable w ...1979227565
surface markers and size of lymphocytes in human umbilical cord blood stimulated into deoxyribonucleic acid synthesis by epstein-barr virus.we characterized subpopulations of lymphocytes in human umbilical cord blood which are stimulated into deoxyribonucleic acid synthesis by epstein-barr virus. lymphocytes were examined simultaneously for deoxyribonucleic acid synthesis by autoradiography and for surface markers by rosette formation with sheep erythrocytes or erythrocytes coated with antibody and mouse complement (eac). the subpopulation which incorporated [3h]thymidine after exposure to virus consisted mainly of cells which forme ...1979227794
[burkitt's lymphoma and epstein-barr virus]. 1979230462
sister chromatid exchanges and chromosomes in chronic myelogenous leukemia and cancer families.spontaneous sister chromatid exchanges and banded karyotypes were studied in blood lymphocytes from 96 individuals: seven patients with chronic myelogenous leukemia, 15 normal controls, and five "cancer families" comprising 12 cancer patients, 40 tumor-free blood relatives and 22 spouses. the families had: malignant melanoma; epstein-barr virus-associated malignancies and a birth defect syndrome; non-hodgkin lymphoma and diverse carcinomas; hodgkin's lymphoma and adenocarcinomas; and acute myelo ...1979282271
antibodies to epstein-barr virus-determined antigens in normal subjects and in patients with seropositive rheumatoid arthritis.prior studies have shown that patients with seropositive rheumatoid arthritis (ra) have an increased frequency of precipitating antibody against a nuclear antigen, the ra nuclear antigen, detected in human b lymphoblastoid cell lines infected by epstein-barr virus. the present investigations demonstrate that patients with seropositive ra also have specifically elevated titers of antibodies to another, better-characterized epstein-barr virus-associated b cell antigen, the epstein-barr nuclear ant ...1979230491
establishment of anti-tnp antibody-producing human lymphoid lines by preselection for hapten binding followed by ebv transformation.human lymphoblastoid cell lines that produce specific antibody against the hapten trinitrophenyl (tnp) have been established by selecting tnp-binding human b lymphocytes by tnp-rosetting and ficoll-isopaque separation, followed by epstein-barr virus (ebv) immortalization. derived lines secreted polyclonal anti-tnp antibodies and contained relatively small numbers of specific rosette- and plaque-forming cells against tnp-rbc. following rerosetting with tnp-rbc, the frequency of rosette-forming ce ...1979230581
hla-d typing with lymphoblastoid cell lines. vi. rationale and goals of data reduction.this report documents various characteristics of hla-d typing by mixed leukocyte culture reactions when lymphoblastoid cell lines (lcls) are substituted for peripheral blood lymphocytes as the stimulator cells. it also provides the rationale for designing the computer program described in the subsequent report. in such experiments, each donor to be hla-d typed is stimulated with a panel of 30-50 hla-d homozygous lcls, each defined hla-d allele being represented by several different human homogyg ...1979230611
epstein-barr virus infection in polytransfused patients with homozygous beta-thalassaemia.the frequency of epstein-barr virus (ebv) and hepatitis b virus (hbv) infection has been studied in 149 polytransfused thalassaemic patients and in healthy controls. evidence for ebv infection was based on the detection of antibodies to viral capsid antigen (anti-vca) and for hbv infection on the detection of either hepatitis b surface antigen (hbsag) or hepatitis b surface antibody (anti-hbs). the frequency of anti-vca was not significantly higher in the patients (16.4%) compared to the control ...1979230639
vsv-inhibitor, interferon and anti-interferon activities in subacute and chronic viral infections. 1979230680
[a specific igm test for the diagnosis of epstein-barr virus infections (author's transl)].igm antibodies specific for epstein-barr virus (ebv) were measured in 302 patients with high igg antibody titers to study whether ebv was the cause of disease in children having one or more symptoms of classical infectious mononucleosis. igm antibodies specific for ebv were found in all patients with the defined clinical picture of infectious mononucleosis. in addition the majority of cases with clinical suspicion of the disease had also specific igm titers. besides infectious mononucleosis ebv ...1979390371
infectious mononucleosis in the elderly.infectious mononucleosis (im) was diagnosed in four patients over the age of 50 years. their age, absence of splenomegaly, lack of significant lymphadenopathy in three and an atypical presentation in one all contributed to a delay in the diagnosis. in two patients, in whom complications occurred, the paul-bunnell test was repeatedly negative. confirmation of the diagnosis was made by the measurement of the epstein-barr virus igm using differential sucrose gradient centrifugation. because the pre ...1979463681
the study of epstein--barr virus induction by cocultivation of ebv-transformed cells with a mammary carcinoma cell line mcf-7. 1979230055
[effect of sex on immunological responsiveness]. 1979230910
cell-mediated lymphocytotoxicity directed against hla-d gene products.cytotoxic effector cells were generated in human mixed lymphocyte cultures (mlcs) between cell donors differing at the hla-d/dr region but not at hla-a,b(c). these cells killed epstein-barr virus-infected lymphoblasts (ebv blasts) from donors sharing the hla-d/dr determinants with the normal mlc-stimulating cells, but not phytohaemagglutinin (pha) lymphoblasts from the same donors. because ebv blasts are derived from b cells and express hla-d/dr antigens, the most likely interpretation of these ...1979432943
histological and electron microscopic studies of endemic ethmoidal carcinomas in cattle.in five cases of endemic ethmoidal carcinoma in cattle from the dominican republic three tumor types could be classified: undifferentiated carcinoma (3), adenocarcinoma (1), and squamous cell carcinoma (1). electron microscopy showed that the tumor cells in undifferentiated carcinomas closely resembled the cells of the normal olfactory mucosa. this was especially true for the dark cells of bowman's gland. ultrastructurally, the lymphoid cells of the undifferentiated bovine carcinoma resembled th ...1979442448
non-a, non-b hepatitis among participants in a plasmapheresis stimulation program.non-a, non-b hepatitis was transmitted to seven of nine participants in a red blood cell-stimulation program following transfusion of blood from one asymptomatic donor. five of the seven patients had clinical as well as biochemical evidence of infection, and three of these five were icteric. incubation periods for the clinical cases ranged from 28 to 50 days, and duration of illness was from two to eight weeks. none of the seven patients showed serologic evidence of acute infection or reinfectio ...1979448190
epstein barr virus (ebv) and complement (c3) interactions with human lymphoid cells. 1979232455
a serological survey of st lucia.a serological survey of a random sample of 541 of the population of st lucia was undertaken. the prevalence of antibodies to dengue, herpes virus, vz, rotavirus, rubella and syphilis is described and compared with other communities.1979232090
intracellular state of epstein-barr virus dna in producer cell lines.the physical state of the epstein-barr virus (ebv) dna in three cell lines which spontaneously produce virus has been characterized. circular ebv dna molecules have been found in p3hr-i, b95-8 and m8i cells. the size of the intracellular m8i circular ebv dna molecules is comparable with the linear virus genome isolated from virus particles but the circular p3hr-i and b95-8 dna molecules are shorter than the virion dna . in addition to the circular form, some ebv dna with physical properties indi ...1979232128
blocking of acid-fixed nuclear binding of epstein-barr virus nuclear antigen (ebna) by different dna species.treatment of epstein-barr virus-determined nuclear antigen (ebna) with dna resulted in blocking of its ability to convert acid-fixed ebna-negative cell nuclei to an ebna-positive form. epstein-barr virus (ebv) dna, herpes simplex virus type 2 (hsv-2) dna and dna isolated from three lymphoblastoid cell lines differed in their potency to block this reaction. ebv dna was found to be about three times more effective than cellular dnas in abolishing the ability of dna-cellulose-purified ebna to conve ...1979231093
psychosocial risk factors in the developmental of infectious mononucleosis.in a 4-year prospective seroepidemiological study of infectious mononucleosis (im) of one class of some 1400 cadets at the west point military academy, susceptibles and immunes were identified by the absence or presence of antibody to epstein-barr virus (ebv), the causative agent, and new infections by the appearance of antibody (seroconversion). on entry, about 1/3 lacked ebv antibody, of whom some 20% became infected (seroconverted); about 1/4 of seroconverters developed definite, clinical and ...1979231279
[the molecular biology of herpes viruses: (part ii) dna structure analysis and latent viral genomes of ebv, mdv, h. saimiri, cmv etc. (author's transl)]. 1979231281
[significance of virus-specific antibodies to ebv-antigens in patients with nasopharyngeal carcinoma (author's transl)].sera collected from patients with nasopharyngeal carcinoma, patients with other head and neck neoplasms, patients with infectious mononucleosis (im), and healthy donors were titrated for antibodies (igm, igg, iga) against epstein-barr-virus (ebv) specific antigens (vca, ea, ebna) by indirect and by antikomplementary immunfluorescence. npc-patients develop significantly high anti-ebv titers of igg and iga. in contrast patients with neoplasms (mainly carcinomas) arising in sites of the head and ne ...1979231716
phosphonoacetic acid: inhibition of transformation of human cord blood lymphocytes by epstein-barr virus.phosphonoacetic acid disodium salt (paa) inhibited the transformation of human cord blood lymphocytes by epstein-barr virus (ebv) at concentrations of 50-100 microgram/ml. at these concentrations, paa had no effect on the multiplication of ebv transformed human lymphoblastoid cells or on the survival of human cord blood lymphocytes. the transformation of human cord blood lymphocytes by the b95-8 strain of ebv was measured by 3h-thymidine uptake, 5 days or more after infection. the degree of inhi ...1979233303
[detection of iga antibody to eb virus vca from patients with nasopharyngeal carcinoma by immunofluorescence test (author's transl)]. 1979233359
[study of giant group a marker chromosome in several burkitt lymphoma and lymphoblastoid cell lines with epstein-barr virus from different origins (author's transl)]. 1979233360
dna glycosylases of human lymphoblastoid cells.lymphoblastoid cell lines were established after transformation by epstein-barr virus of peripheral lymphocytes from xeroderma pigmentosum (xp) patients and normal donors. these lines expressed b-lymphocyte characteristics. typical characteristics related to xp of these cell lines were not altered by transformation. extracts of these cells catalyzed release of uracil (ura) and 3-methyladenine (3meade) from ura-containing dna (ura-dna) and methylated dna (me-dna), respectively. these two activiti ...1979232757
the possible role of epstein-barr virus in rheumatoid arthritis.the sera of patients with rheumatoid arthritis form a precipitate with an antigen present in the sonicate of a human b (bone marrow-derived) lymphoid cell line (wil2). the antibody is distinct from rheumatoid factor. the antigen can be demonstrated by an immunofluorescence technique to be distributed within the nucleus of the wil2 cell and shows a discrete speckled pattern of nuclear staining. the nuclear antigen is not detectable in organ extracts from many animal species or in human t (thymus- ...1979233172
infection and persistence of varicella-zoster virus in lymphoblastoid raji cell line.infection of raji cells by varicella-zoster virus (vzv) resulted in permissive infection with establishment of a persistently infected lymphoblastoid cell line. vzv antigens of the membrane and nuclear type, as detected by the indirect immunofluorescence membrane antigen (ifama) and anticomplement immunofluorescence (acif) tests, were observed. minute amounts of infectious virus were detected by co-cultivation of vzv-infected raji cells (raji-vzv), with permissive human embryo fibroblasts (hef). ...1979232537
acute non-a, non-b hepatitis. prolonged presence of the infectious agent in blood.non-a, non-b hepatitis, previously transmitted to chimpanzees by inoculation of human serum, was serially transmitted through a second and third passage to additional chimpanzees using serum drawn during acute non-a, non-b hepatitis. sera obtained at weeks 4 and 5 after inoculation from two different chimpanzees, and from one chimpanzee at week 13 after inoculation, were shown to cause elevation of serum aminotransferase levels and abnormal liver biopsies in recipient chimpanzees, with no serolo ...1979421994
association of human chromosome 14 with a ts defect in g1 of chinese hamster k12 cells.somatic cell hybrids between human lymphoblastoid cells (raji) and temperature-sensitive chinese hamster cells (k12) were selected from monolayer cultures in mem at 40 degrees c. a total of 21 hybrid clones were isolated and karyotyped. all clones contained a near complete set of chinese hamster chromosomes and 1 to 5 human chromosomes. human chromosome 14 present in the hybrid cells of all clones; and was the only human chromosome retained in 10 clones. the presence of human chromosome 14 in hy ...1979455493
2/8 translocation in a japanese burkitt's lymphoma.a new translocation between chromosomes 2 and 8, t(2p-; 8q+), was found in fresh lymphoma cells from a japanese patient with epstein-barr virus-carrying burkitt's lymphoma, and in a lymphoma cell line derived from this patient. there was no 14q+ translocation, as has been previously described in african and north american burkitt's lymphomas.1979467575
remission of giant lymph node hyperplasia with anemia after radiotherapy.we have described a 51-year-old patient with unresectable mesenteric giant lymph node hyperplasia of the plasma cell type, severe systemic manifestations, and profound anemia. supression of erythropoiesis may have been related to the presence of a circulating erythropoietic inhibitor produced by the lymphoid tumor. markedly elevated titers to epstein-barr virus capsid antigen suggest that this virus may be important in the etiology of the abnormal lymphoid proliferation. the marked clinical resp ...1979476562
cultured "hairy cells" infected with epstein-barr virus: evidence for b-lymphocyte origin. 1979311181
cytogenetic study of a european burkitt's lymphoma cell line.the chromosomes of an epstein-barr virus-negative european burkitt's lymphoma cell line were studied. all the cells carried the t(8;14) translocation. one clone had 51 chromosomes and was (+1,+7,+16,+15,+21), whereas another clone also had 51 chromosomes but was (+1q+,+7,+16,+15,+21). a third clone had 46 chromosomes (4q+/-).1979286095
cell culture studies in progeria. i. establishment and partial characterization of a lymphoblastoid cell line.a lymphoblastoid cell line (eks-l44) with definitive hla specificities (hla-aw24, w30, b14, w35, cw4-, drw4, 5) was established from peripheral blood cells of a patient with progeria using epstein-barr virus. cytogenetic study indicated that eks-l44 was diploid after four months in vitro. the growth pattern of this cell line was not significantly different from a similarly established normal control nor from other lines initiated from patients with various disorders.1979513854
anti-rana antibody and rheumatoid arthritis. 19806102704
who will get leukaemia? 19806103331
epstein barr virus nuclear antigen positive lymphoma after cyclosporin a treatment in patient with renal allograft. 19806104142
binding of sv40 tumour antigen and epstein-barr-virus nuclear antigen to isolated acid-fixed nuclei.sv40 tumour antigen (ta) bound to methanol-acetic-acid-fixed interphase nuclei and metaphase chromosomes. this reaction was visualized by the anti-complement immunofluorescence test. the reaction was negative when the nuclei had been treated with deoxyribonuclease prior to the addition of antigen, or when ta-antibody negative serum had been used. in parallel tests ta and epstein-barr virus nuclear antigen were examined. both the binding patterns and the optimum conditions for the reaction were s ...19806107019
infectious mononucleosis and acute monocytic leukemia. 19806107449
hepatic function in mononucleosis induced by epstein-barr virus and cytomegalovirus.we evaluated serial enzyme and bilirubin determinations as aids to diagnosis of epstein-barr virus-induced infectious mononucleosis (121 cases) and the heterophil-negative mononucleosis-like illness due to cytomegalovirus (33 cases). laboratory evidence for either type of mononucleosis includes mild to moderate hepatic dysfunction, with aspartate aminotransferase activity increased, but lower than commonly encountered in active viral hepatitis. of the enzymes commonly assayed in evaluating liver ...19806101548
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