PMID(sorted ascending)
different localization of epstein-barr virus genome in two subclones of the burkitt lymphoma cell line namalwa.the epstein-barr virus genome contained in the burkitt lymphoma line namalwa was previously localized to the short arm of chromosome 1. analysis of a different subline of the same namalwa line by means of southern analysis carried out on genomic dna, as well as in situ hybridization, showed a localization on the x chromosome.19921382560
expression of wild-type and mutant medium-chain acyl-coa dehydrogenase (mcad) cdna in eucaryotic effective ebv-based expression system for eucaryotic cells has been developed and used for the study of the mitochondrial enzyme medium-chain acyl-coa dehydrogenase (mcad). 1325 bp of pcr-generated mcad cdna, containing the entire coding region, was placed between the sv40 early promoter and polyadenylation signals in the ebv-based vector. both wild-type mcad cdna and cdna containing the prevalent disease-causing mutation a to g at position 985 of the mcad cdna were tested. in transfected cos ...19921382617
expression of tumor-associated epitopes on epstein-barr virus-immortalized b-cells and burkitt's lymphomas transfected with epithelial mucin complementary dna.mucins are among the best described human tumor-associated antigens. at least 73 tumor-reactive anti-mucin antibodies have been described; in addition, we have previously demonstrated the existence of tumor-specific cytotoxic t-lymphocyte epitopes on the mucin produced by breast and pancreatic tumors. to determine whether the appearance of tumor-associated mucin epitopes can be explained by altered post-translational modification of mucin in tumors, or whether the generation of these epitopes re ...19921382849
paroxysmal nocturnal hemoglobinuria due to hereditary nucleotide deletion in the hrf20 (cd59) gene.hrf20 (cd59) is a membrane glycoprotein which protects cells from the membrane attack reaction of homologous complement. a patient who is completely deficient in hrf20 expression and is suffering from paroxysmal nocturnal hemoglobinuria (pnh) was studied. his parents are cousins and both have decreased hrf20 expression, suggesting that the deficiency is genetic. we established a cultured cell line (ncu1) which is hrf20 deficient from the patient's lymphocytes by epstein-barr-virus (ebv) infectio ...19921382994
characterization of an epstein-barr virus receptor on human epithelial cells.epstein-barr virus (ebv) adsorption to human b lymphocytes is mediated by the viral envelope glycoprotein, gp350/220, which binds to the cell surface protein, cd21, also known as the cr2 complement receptor. human epithelial cells also express an ebv receptor. a candidate surface molecule of 195 kd has previously been identified on an epithelial cell line and explanted epithelial tissue by reactivity with the cd21 specific monoclonal antibody (mab), hb-5a. in experiments to further characterize ...19921383386
detection of, and anti-collagen antibody produced by, cd5-positive b cells in inflamed gingival tissues.this study was performed to investigate the frequency and distribution of cd5-positive (cd5+) b cells in inflamed gingival tissues using flow cytometric and immunohistochemical analyses. the ability of cd5+ b cells to produce anti-type i collagen antibody was also examined. cd5+ b cells expressed "low" fluorescence intensity in the peripheral blood of both healthy subjects and patients with adult periodontitis. however, in inflamed gingival tissues the intensity of this surface marker was high. ...19921383487
transfer of human chronic lymphocytic leukemia to mice with severe combined immune deficiency.b chronic lymphocytic leukemia (cll) cells were transferred into mice with severe combined immunodeficiency (scid). leukemia cells injected into the peritoneal cavity of these animals may survive for at least 10 weeks in vivo. in contrast, leukemia cells do not survive for long periods when injected intravenously. despite the longevity of cll cells injected i.p., these cells apparently do not migrate to other lymphoid tissues. eight to sixteen weeks after receiving cll cells, scid mice develop h ...19921383641
igm kappa/lambda ebv human b cell clone: an early step of differentiation of fetal b cells or a distinct b lineage?in agreement with the clonal theory, one b lymphocyte synthesizes one antibody due to allelic and isotypic exclusion. we analyzed an ebv b-cell clone, e29.1, derived from an 11 week-old embryo, and secreting both igm kappa and igm lambda. structural analysis of produced igm, indicated that lambda-containing pentamers could be considered hybrid molecules, expressing both the kappa and lambda. chains, with a kappa/lambda ratio between 5 and 10. it was also found that 60% of the lambda chains were ...19921383695
description of a new amplifiable shuttle vector for mutagenesis studies in human cells: application to n-methyl-n'-nitro-n-nitrosoguanidine-induced mutation order to analyze the mechanisms of mutagenesis in human cells, we have established a human 293 cell-derived line containing a permanent mutagenesis target, the bacterial lacz' gene, on an episomal ebv/sv40-based shuttle vector. this plasmid was maintained at a low copy number per cell which rendered it closer to an endogenous gene as compared to the usual transient shuttle vectors. transient amplification of vectors, inside the host cell due to expression of the sv40 t-antigen, allowed the re ...19921383742
molecular mimicry and the autoimmune response to the peripheral nerve myelin p0 the lewis rat immunisation with the myelin p0 glycoprotein can induce an inflammatory demyelinating disease of the peripheral nervous system, experimental allergic neuritis (ean), which has many clinical and histopathological parallels with the human disease the guillain-barre syndrome. in view of the reported association of gbs with a number of infectious agents we have investigated whether "molecular mimicry" may occur between microbial antigens and the p0 protein that could possibly trigge ...19921383842
chronic lymphocytic leukemia/small lymphocytic lymphoma with reed-sternberg-like cells and possible transformation to hodgkin's disease. mediation by epstein-barr virus.the pathogenesis of reed-sternberg cells and variants (rs-h cells) found in rare cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (cll/sll) is unknown. we studied 13 such cases by immunohistochemistry and in situ hybridization for identification of epstein-barr virus (ebv) rna. the rs-h cells in five cases expressed the b-lineage marker cd20 and were negative for cd15. in two cases, the rs-h cells showed expression of both cd20 and cd15, whereas in another six cases, the cells we ...19921384376
lymphoepitheliomalike carcinoma of the skin. a case report with immunophenotypic analysis and in situ hybridization for epstein-barr viral genome.lymphoepithelioma is a malignant epithelial neoplasm of the nasopharynx. similar malignancies--lymphoepitheliomalike carcinomas--of salivary glands, thymus, larynx, lung, stomach, and uterus have been described. we present here a case of lymphoepitheliomalike carcinoma of the skin in an 84-year-old woman. histologically this neoplasm presented as a fairly discrete dermal aggregate of syncytial nests of epithelioid-appearing cells that displayed no squamous or glandular differentiation, surrounde ...19921384378
expression of the cd2 molecule on human b lymphoid progenitors.cd2 expression on human b lymphoid progenitor cells was examined. by immunofluorescence analysis, a small fraction of bone marrow b cells was found to express cd2 on their surface. cd2 expression was not demonstrated on peripheral b cells. epstein-barr virus-transformed b cell lines derived from fetal liver at 8 weeks of gestation were analyzed to delineate the expression and function of cd2 at the early stage of human b cell development. characterization of surface and genomic phenotypes of cel ...19921384685
epstein-barr virus burden in hodgkin's disease is related to latent membrane protein gene expression but not to active viral replication.the epstein-barr virus (ebv) has been increasingly detected in hodgkin's disease (hd), but its role in pathogenesis remains uncertain. we analyzed 20 specimens of hd known to contain ebv dna by a sensitive reverse transcriptase polymerase chain reaction (rt-pcr). the cases were assessed for the presence of rna transcripts of the bnlf1 gene (coding for the viral latent membrane protein [lmp]) and the late replicative gene bllf1 (coding for the principle envelope glycoprotein [gp220/350]). lmp rna ...19921384793
viral hepatitis in the 1990s, part iii: hepatitis c, hepatitis e, and other viruses.acute hepatitis can be caused by a number of viruses, especially a, b, c, e, delta, epstein-barr virus, and cytomegalovirus. hepatitis a and b have been discussed previously in this series. the virus responsible for most cases of what commonly has been referred to as non-a non-b hepatitis has been tracked, and antibodies to certain proteins of this virus have been identified. this virus is now referred to as hepatitis c. the possible clinical outcomes after acute hepatitis c virus infection are ...19921385012
mapping of an epitope defined by a human hybridoma antibody (trd3): a new hla-b supertype associated with a subset of hla-bw6.the new human-human hybridoma trd3 secretes a cytotoxic igm mab, which reacted with 28 of a panel of 56 hla-typed lymphoblastoid cells. all 28 trd3+ cells expressed the hla-b supertype bw6, whereas 10 bw6+ cells were not recognized by the mab. none of the 17 bw4 homozygous cells were positive with trd3. thus, trd3 divided the bw6+ hla-b specificities of the cell lines into two subgroups, namely, bw6+trd3+ and bw6+trd3-, and therefore defines a new hla-b supertype. trd3 reacted strongly with some ...19921385373
peptide-induced nonresponsiveness of hla-dp restricted human t cells reactive with dermatophagoides spp. (house dust mite).the activation of cd4+ t lymphocytes, which play a central role in allergic inflammation, depends on the recognition of allergen-derived peptides in association with major histocompatibility complex class ii gene products. in this report we demonstrate, at a clonal level, that a component of the t-cell repertoire reactive with dermatophagoides spp. (house dust mite) in atopic individuals, is restricted by hla-dp class ii molecules. this supports the recent results emerging from genetic epidemiol ...19921385507
human monoclonal antibodies against an epitope on the class 5c outer membrane protein common to many pathogenic strains of neisseria meningitidis.neisseria meningitidis is a causative agent of meningitis. despite vaccination programs, it still causes a large number of deaths in young children. early diagnosis followed by passive immunization with human monoclonal antibodies could be an approach to effective therapy. peripheral blood lymphocytes from normal, healthy blood donors and from vaccinated individuals were immunized in vitro, using outer membrane proteins purified from n. meningitidis b:4:p1.15. the immunized human b cells were ep ...19921385548
bacteriophage cloning and escherichia coli expression of a human igm fab.we have combined the molecular biology methods of the polymerase chain reaction and recombinant dna cloning in bacteriophage lambda to express a human igm fab in escherichia coli using genes derived from an epstein-barr virus transformed cell line. this method comprises three cdna amplifications and a single cloning step, culminating in the stable overexpression of mammalian heterodimeric recombinant protein in a prokaryotic host.19921385989
human helper t cell lines established by coculture of normal human cord leukocytes with an htlv-ii-infected rabbit leukocyte cell line (ra-iia).three helper t cell lines, designated cr-iia (cr-iia-1, cr-iia-2, and cr-iia-3), were established by coculturing normal human cord leukocytes with a lethally irradiated htlv-ii (human t-lymphotropic virus type ii)-infected rabbit leukocyte cell line (ra-iia). cr-iia had a normal human karyotype and expressed the surface markers cd3(+), cd4(+), cd8(-), cd19(-), cd25(+) and hla-dr(+), confirming their helper t cell nature. cr-iia cells were all free of epstein-barr virus nuclear antigen and were i ...19921386172
cd21 is a ligand for cd23 and regulates ige production.the molecule cd23, a low-affinity receptor for ige (fc epsilon r2), is a type ii transmembrane molecule expressed on many haemopoietic cell types. cd23 has pleiotropic roles in the control of lymphocyte behaviour, suggesting that cd23 may interact with another ligand in addition to ige. to identify such a cd23 ligand, we expressed and purified full-length recombinant cd23, incorporated it into fluorescent liposomes and used these as a probe. we report here that fluorescent liposomes carrying cd2 ...19921386409
cloning and characterization of the human muscle phosphofructokinase gene.a 35-kbp region of genomic dna encoding the human muscle phosphofructokinase (hpfk-m) gene including all of the coding exons (1-22) plus 2.2-kbp of 5'-flanking sequence has been cloned. the exon boundaries are the same as has been observed for the rabbit muscle phosphofructokinase (rpfk-m), the human liver phosphofructokinase (hpfk-l), and the mouse liver phosphofructokinase (mpfk-l) genes. characterization of the structure of the hpfk-m gene and its transcript in epstein-barr virus transformed ...19921388024
fireside conference 19. wegener's granulomatosis and lethal midline summary, wegener's granulomatosis is a systemic illness that is characterized pathologically by necrotizing granulomatosis and vasculitis. lethal midline granuloma is not a pathological term. lethal midline granuloma is a clinical term, and in reality, lethal midline granuloma has been proven to be polymorphic reticulosis and not wegener's granulomatosis. polymorphic reticulosis is now understood to be a t-cell lymphoma. further study of these malignant lymphomas has shown that these t-cell l ...19921389398
alice in wonderland syndrome as an initial manifestation of epstein-barr virus infection.we present a patient with serologically confirmed epstein-barr virus (ebv) infection who had illusions of size, shape, and colour of objects but none of the typical symptoms and signs peculiar to infectious mononucleosis (im) except sore throat which developed 2 weeks after the initial visual disturbances. the bizarre feelings about the images of body and objects are called the 'alice in wonderland syndrome' due to the similarity with alice's dreams. the same symptomatology including visual meta ...19921390519
viruses other than hiv and non-hodgkin's lymphoma.there are several viral infections which are known to cause lymphoma among animals; all establish latency in lymphoid cells. the human t-lymphotropic virus type i is a human virus which causes lymphomas among a subset of carriers. however, this virus is very restricted in its distribution and as such, is unlikely to play a role in the increase of non-hodgkin's lymphoma (nhl). a highly prevalent infection, the epstein-barr virus (ebv) is known to play a role in the etiology of nhl among persons w ...19921394158
molecular basis of lymphomagenesis.lymphoid neoplasms, like all malignant tumors, arise as a consequence of the accumulation, in a single cell, of a set of genetic lesions that result in altered proliferation or increased clonal life span. the most frequently observed genetic abnormalities among the malignant non-hodgkin's lymphomas are translocations, which appear to be lineage and, to a large extent, lymphoma specific. recombinases that normally mediate the process of antigen receptor gene rearrangement appear to have an import ...19921394168
the past is prologue: use of serum banks in cancer this paper, we emphasize the uses of serum banks in cancer research. these include not only case/control studies but also prospective seroepidemiological studies in which the development of a serological marker, such as a viral antibody or viral antigen, can be correlated with the subsequent development of cancer in either an active surveillance program or the use of cancer registries or hospital records. several different methods of application of the cohort technique are illustrated by stud ...19921394173
applications of experimental techniques to epidemiological studies of non-hodgkin's lymphoma: use of archival tissues.archival tissues, particularly formalin-fixed paraffin-embedded tumors, have become increasingly valuable in studies of the etiology of cancer. in non-hodgkin's lymphoma, subclassification of tumors by immunophenotyping and identification of oncogenic viruses has allowed more accurate interpretation of associated epidemiological information. one such example is adult t-cell leukemia/lymphoma, which is not a single histopathological entity and usually is associated with human t-cell lymphotropic ...19921394174
suppression of interleukin-2 and interleukin-2 receptor expression in jurkat cells stably expressing the human immunodeficiency virus tat protein.the jurkat t cell line was stably transfected with an epstein-barr virus-based episomal replicon designed to express high levels of the hiv-1 tat protein. after selection in hygromycin b, high-level tat activity was detected in 3 of 18 transfected cell lines. after stimulation with phytohemagglutinin (pha) and phorbol myristate acetate (pma), tat transfectants with high tat expression showed diminished expression of interleukin-2 (il-2) and the interleukin-2 receptor alpha chain (il-2r) when com ...19921394441
a trypsin-like serine protease activity on activated human b cells and various b cell lines.we have studied the trypsin-like serine protease activity of human tonsillar b lymphocytes. the lysate of the low-density, in vivo activated b cells as well as the lysate of cells stimulated with anti-human igm f(ab')2 show elevated trypsin-like serine protease activity compared to the resting subset as monitored by the cleavage of tos-gly-pro-arg-pna. the cleavage is sensitive to n-tosyl-l-lysyl-chloromethyl ketone and benzamidine but not to iodoacetamide. experiments with intact cells give sim ...19921396962
benign lymphocytic angiitis and granulomatosis: a t-cell lymphoma?benign lymphocytic angiitis and granulomatosis is a t-cell lymphoproliferative disorder confined to the lung and corresponding to a low-grade angiocentric immunoproliferative lesion. controversy remains as to whether these lesions are lymphomas. we report such a case in an 8-year-old patient with burkitt's lymphoma in remission who presented with persistent bronchopneumopathy and bilateral pulmonary infiltrates on tomodensitometry. surgical resection revealed the histologic changes of benign lym ...19921398650
adult t-cell leukaemia-derived factor/thioredoxin expression on the htlv-i transformed t-cell lines: heterogeneous expression in alt-2 t-cell leukaemia (atl)-derived factor (adf), originally described as an inducer of interleukin-2 receptor-alpha (il-2r alpha/tac), has homology with the co-enzyme thioredoxin which is involved in many dithiol-dependent reducing processes. using antibody against the c-terminal synthetic polypeptide of adf and rna probe of adf, we examined the expression of adf in various cell lines by immunofluorescence, immunohistochemical staining, western blotting and in situ hybridization. adf was inten ...19921398748
nomenclature for factors of the hla system, 1991. 19921399721
primary central nervous system lymphomas--an update.primary cns lymphomas (pcnsl), until recently representing about 1% of all brain tumors, show dramatically increased incidence both in high-risk groups (immunocompromised, aids) and in the general population. they are extranodal diffuse non-hodgkin's lymphomas, the morphology and classification of which are identical to those of systemic lymphomas, although pcnsl show different biological behavior and diagnosis according to the new working formulation and updated kiel classification may be diffi ...19921400570
extensive and selective mutation of a rearranged vh5 gene in human b cell chronic lymphocytic leukemia.b cell chronic lymphocytic leukemia (cll) is the malignant, monoclonal equivalent of a human cd5+ b cell. previous studies have shown that the vh and vl genes rearranged and/or expressed in cll have few and apparently random mutations. however, in this study, we have found that the rearranged vh251 gene, one of the three-membered vh5 family, has extensive and selective mutations in b-cll cells. somatic mutation at the nucleotide level is 6.03% in b-clls whereas the somatic mutation levels are mu ...19921402653
epstein-barr virus latent membrane protein transactivates the human immunodeficiency virus type 1 long terminal repeat through induction of nf-kappa b activity.the epstein-barr virus latent membrane protein (lmp) is an integral membrane protein that is expressed in cells latently infected with the virus. lmp is believed to play an important role in epstein-barr virus transformation and has been shown to induce expression of several cellular proteins. we performed a series of experiments that demonstrated that lmp is an efficient transactivator of expression from the human immunodeficiency virus type 1 long terminal repeat (hiv-1 ltr). mutation or delet ...19921404600
role of interleukin 6 in the growth of myeloma-derived cell lines.the role of interleukin 6 (il-6) in the growth of five multiple myeloma-derived cell lines was characterized. the u266 and rpmi 8226 cell lines demonstrated increased dna synthesis when cultured with exogenous il-6, expressed il-6 cell surface receptors (il-6rs) and expressed mrna for il-6r. however, these cells did not secrete detectable il-6 protein, and a neutralizing antibody to il-6 did not inhibit their growth. three other myeloma-derived cell lines arh-77, im-9 and hs-sultan did not respo ...19921405708
the ebna2-related resistance towards alpha interferon (ifn-alpha) in burkitt's lymphoma cells effects induction of ifn-induced genes but not the activation of transcription factor isgf-3.transfection of a plasmid encoding the epstein-barr virus (ebv) nuclear antigen 2 (ebna2) gene confers resistance to the antiproliferative effect of alpha interferon (ifn-alpha) in ebv-negative u968 cells (p. aman and a. von gabain, embo j. 9:147-152, 1990). we studied the expression of ifn-stimulated genes (isgs) in two pairs of burkitt's lymphoma cell lines, differing in the expression of the putative immortalizing gene of ebv, ebna2. in ebna2-expressing cells, the induction of four isgs by if ...19921406670
[the cervicofacial manifestations of kaposi's sarcoma and of non-hodgkin's lymphomas in hiv-infected patients].infection from human immunodeficiency virus (hiv) is well known for the particular host susceptibility to a variety of opportunistic infections and unusual malignant neoplasms. although no tumor develops exclusively in concomitance with hiv infection, malignancies in these patients have different clinical behaviour, response to treatment and prognosis than the pattern observed in hiv negative hosts. kaposi's sarcoma (eks) and non-hodgkin's lymphoma (nhl) are tumors per se diagnostic of aids in p ...19921414319
diversity in dna rearrangements and in rna expressions of immunoglobulin gene on common variable immunodeficiency.six heterogeneous common variable immunodeficiency (cvid) patients were analysed for germ-line dna, dna rearrangements, and rna expressions of immunoglobulin (ig) gene by southern or northern blotting using appropriate probes. we detected no polymorphism in neutrophil dna hybridized to a c mu and a c gamma probe. in three patients, both serum ig and ig-bearing cells were scarcely detected, and by northern hybridization methods, neither mu mrna, gamma mrna, alpha mrna nor kappa mrna was detected. ...19921420114
identification and synthesis of a novel 15 kda beta-galactoside-binding lectin in human leukocytes.knowledge of the identity, synthesis and secretion of beta-galactoside-binding lectins by leukocytes is of importance because lactosaminoglycans present at the leukocyte cell surface may be physiologically significant lectin receptors that could mediate autocrine or paracrine functions and/or cell adhesion. this paper presents data that show that a previously identified 15.5-16.5 kda lactose-binding protein synthesized in vitro by human peripheral leukocytes is actually comprised of three differ ...19921421750
proliferative dependent regulation of the glyceraldehyde-3-phosphate dehydrogenase/uracil dna glycosylase gene in human cells.the relationship between the proliferative dependent expression of the glyceraldehyde-3-phosphate dehydrogenase (gapdh)/uracil dna glycosylase (udg) gene and the induction of uracil dna glycosylase activity was examined in human cells. three different cell types were studied to determine whether the growth-dependent regulation of this multifunctional gene was a common characteristic of human cells. these included wi-38 normal embryonic lung fibroblasts, a japanese bloom's syndrome non-transforme ...19921423884
t lymphocyte expression of complement receptor 2 (cr2/cd21): a role in adhesive cell-cell interactions and dysregulation in a patient with systemic lupus erythematosus (sle).complement receptor 2 (cr2, cd21), the receptor for both the c3d,g portion of human complement component c3 and the epstein-barr virus, has been recently described on peripheral t cells. by using dual stain flow cytometric analysis, we have also observed that a peripheral t lymphocyte subpopulation of normal healthy donors bears cr2 in a range varying from 1.1 to 23.2% (mean 12.6%) of total cd3+ cells. t lymphocytes from nine patients with inactive sle expressed cr2 in a similar range. three pat ...19921424280
presence and significance of cold agglutinins in patients with hiv patients may contract a variety of other concurrent infections, including mycoplasma pneumoniae, cytomegalovirus (cmv) and epstein-barr virus (ebv), that are known to be causes of transient cold agglutinins (ca). the aim of this study was to search for the prevalence, the persistence and the significance of ca in hiv antibody-positive patients.19921427429
alternative exon usage and processing of the major histocompatibility complex-encoded proteasome subunits.the finding that two subunits of the proteasome, lmp2 and lmp7, are encoded in the major histocompatibility complex (mhc) has linked the proteasome which represents a major extralysosomal proteolytic system to the processing of intracellular antigens. here we describe a second form of the human lmp7 cdna, lmp7-e2, which has been identified during the characterization of novel genes in the mhc. the analysis of the genome organization of lmp7 revealed that lmp7-e1 and lmp7-e2 arise by alternative ...19921429565
constitutive production of interferon-alpha 2 by a human b-lymphoblastoid cell line.human b-lymphoblastoid cell lines immortalized by epstein-barr virus constitutively produce low levels of acid-stable interferon (ifn) that differs in composition from ifn produced by the same cell type after virus induction. we use three approaches to study the constitutive ifn produced by the lymphoblastoid cell line, lukii: (i) antiviral bioassays with a neutralizing monoclonal antibody; (ii) amino-terminal sequence analysis of the single protein obtained after purification of the ifn by immu ...19921431307
immunocompetent cell markers in human fetal astrocytes and neurons in culture.during the past few years, evidence has accumulated that interaction with peripheral immune cells as well as immunoregulatory functions in the central nervous system (cns) can be played by several types of brain resident cells. since very little information is available in man, however, we investigated the presence of markers so far considered typical of immunocompetent cells in in vitro cultures of human fetal brain. immunocytochemistry at the light, scanning, and transmission electron microsco ...19921433389
[function, molecular structure and gene expression regulation of interleukin-10 (il-10)].interleukin-10 has a variety of biological activities. murine interleukin-10 inhibits cytokine production by th2 cells in the presence of macrophages, enhances t cell proliferation, sustains the viability of b cells in vitro, induces class ii mhc antigen expression on b cells, enhances mast cell proliferation in the presence of il-3 and/or il-4, and inhibits cytokine production by macrophages. human interleukin-10 inhibits cytokine production by human t cells and reduces antigen-specific human t ...19921433975
proteasome subunits encoded in the mhc are not generally required for the processing of peptides bound by mhc class i molecules.antigen processing provides major histocompatibility complex (mhc) class i molecules with short peptides, which they selectively bind and present to cytotoxic t lymphocytes. the proteolytic system generating these peptides in the cytosol is unidentified, but their delivery into the endoplasmic reticulum is mediated by the tap1-tap2 transporter encoded in the mhc class ii region. closely linked to tap1 and tap2 are genes for the lmp2 and lmp7 proteins, which resemble components of proteasomes, pr ...19921436094
viral hepatitis in the christchurch determine the relative frequency of known causes of viral hepatitis in the christchurch community.19921436840
intranuclear sorting and assembly of proteins. 19921446350
[bacterial adhesion to epithelial cells of the nasopharynx essential in the development of otitis media].otitis media develops when certain bacterial pathogens gain access to the middle ear cavity from the nasopharynx through the eustachian tube. adhesion of bacteria, in particular streptococcus pneumoniae and haemophilus influenzae, to the non-ciliated epithelial cells of the nasopharynx, close to the opening of the eustachian tube, is significantly correlated to the otitis-prone condition in children. otitis-prone children have significantly fewer bacteria in the nasopharynx coated with the immun ...19921448342
dna binding activity and inhibition of dna-protein interactions. differential effects of tetra-p-amidino-phenoxyneopentane and its 2'-bromo the present study are reported the differential dna binding activity of the anti-tumor polyamidine tetra-p-amidinophenoxyneopentane (tapp-h) and its 2'-halo derivative (tapp-br), and their effects on the binding of the recombinant epstein-barr virus (ebv) nuclear antigen to a synthetic oligonucleotide mimicking the target dna sequence present in the ebv genome. in addition, the proliferation kinetics and cell cycle analysis of human leukemia k562 cells treated with tapp-h and tapp-br are repo ...19921449517
longitudinal study of homosexual couples discordant for hiv-1 antibodies in the baltimore macs study.thirty-six sexually active couples serologically discordant for human immunodeficiency virus, type 1 (hiv-1), within the baltimore multicenter aids cohort study (macs) were assessed to determine whether evidence of hiv-1 infection could be detected in the hiv-1-antibody-negative partners and whether factors associated with lack of transmission of hiv from the seropositive to the seronegative partner could be ascertained. six hiv-1 seropositive couples and 18 seronegative couples were followed co ...19921453331
lfa-1 adhesion molecules are not involved in the early stages of hiv-1 env-mediated cell membrane fusion.a recently developed sensitive assay to examine the early stages of hiv-1 env-mediated cell fusion is based on the redistribution of fluorescent dyes between membranes and cytoplasm of adjacent cells, monitored by fluorescence video microscopy. this assay demonstrated that membrane fusion can occur under conditions where no syncytia are formed. fusion started earlier than syncytia formation and was not very sensitive to hiv-1 env+/cd4+ cell ratios. in the current study, this assay was used to de ...19921457205
pathology of malignant lymphomas.although the subject is now seldom formally addressed, much of the pathologic research into malignant lymphoma is still tacitly directed at developing a rational and reproducible classification. pure morphology, while remaining of critical importance in the diagnosis of malignant lymphomas, has been exhausted as a means of understanding the biology of these tumors, which must be the eventual basis of a firm, enduring and clinically relevant classification. thus, histopathologists have turned fir ...19921457495
biologic aspects of human immunodeficiency virus-related lymphoma.a high frequency of lymphoma in human immunodeficiency virus-infected individuals has been reported since the outbreak of the acquired immunodeficiency syndrome (aids) epidemic in 1982. in the vast majority of cases, these lymphomas are highly aggressive b-cell, non-hodgkin's lymphoma of intermediate or high grade of malignancy. aids-associated non-hodgkin's lymphoma are histologically classified as small noncleaved cell lymphoma, large cell immunoblastic plasmacytoid lymphoma, or large noncleav ...19921457505
human immunodeficiency virus as a risk factor in miscellaneous cancers.the association of malignancies, such as non-hodgkin's lymphoma and kaposi's sarcoma, with human immunodeficiency virus infection has been recognized since the beginning of the epidemic. however, an increasing number of tumors not diagnostic of acquired immunodeficiency syndrome has been described in this setting. taking into consideration that survival of patients with human immunodeficiency virus infection is increasing because of improvement of supportive care and better control of human immu ...19921457506
antibody mediated enhancement of hiv-1 infection of an ebv transformed b cell line is cd4 dependent.low levels of anti-viral antibodies may facilitate virus infection of fc-receptor bearing cells. for human immunodeficiency virus (hiv) it has been reported that antibodies can enhance infection of phagocytic cells. we show that hiv-1 can infect an epstein-barr virus transformed b cell line and that low levels of anti-hiv antibodies enhance infection. the enhanced infection was characterized by an increase in viral dna and increased hiv p24 protein production. detection of cell surface antigen e ...19921459171
analysis of two human monoclonal antibodies against melanoma.b cells derived from peripheral-blood lymphocytes (pbl) and tumor-infiltrating lymphocytes (til) from a patient with a high serum antibody titer to autologous melanoma were transformed with epstein-barr virus (ebv) and evaluated for reactivity against autologous tumor. b cells producing antibody reactive with autologous tumor and unreactive with normal fibroblasts were detected both in til and in pbl. one cell line derived from pbl and another derived from til sustained production of tumor-react ...19921459738
lymphoproliferative disease in human peripheral blood mononuclear cell-injected scid mice. i. t lymphocyte requirement for b cell tumor generation.mechanisms of tumor development were studied in scid mice injected with human lymphoid cells from epstein-barr virus-positive (ebv+) donors. about 80% of peripheral blood mononuclear cell (pbmc)-injected animals developed a lymphoproliferative disease associated with oligoclonal ebv+ tumors of human b cell origin. no change in tumor development rate occurred when monocyte-depleted pbmc were inoculated. no tumors developed when purified b cells were injected. b cell lymphoproliferative disease wa ...19921460431
successful plasmapheresis in corticosteroid-resistant hemolysis in infectious mononucleosis: role of autoantibodies against triosephosphate isomerase.a 19-year-old girl fell ill with a high temperature and cervical lymphadenopathy. the detection of heterophile antibodies as well as epstein-barr-virus-specific antibodies confirmed the diagnosis of infectious mononucleosis. in the course of the infection, the patient developed severe hemolytic anemia with her hemoglobin falling from 14 to 8 g/dl. high-dose corticosteroid therapy did not stop hemolysis; this could only be achieved by seven plasmapheresis sessions. antibodies against triosephosph ...19921466197
hairy leukoplakia with involvement of the buccal mucosa.oral hairy leukoplakia occurs mainly on the tongue of human immunodeficiency virus (hiv)-infected persons. an hiv-infected patient with hairy leukoplakia involving the tongue and buccal mucosa was studied by light and electron microscopic methods, in situ hybridization, and polymerase chain reaction. our findings indicate that hairy leukoplakia may involve the buccal mucosa and should be considered in the differential diagnosis of white oral lesions in hiv-positive patients. epstein-barr virus p ...19921469145
murine gammaherpesvirus 68 establishes a latent infection in mouse b lymphocytes in vivo.murine gammaherpesvirus 68 (mhv-68) is able to persist in spleen cells of infected mice. to determine the cell type harbouring persistent virus, spleen cells from infected animals were separated into immunoglobulin (ig)-positive (b cell-enriched), ig-negative (t cell-enriched) and plastic-adherent (macrophage-enriched) fractions. these cells were co-cultivated with permissive bhk-21 cells in an infectious centre assay. the consistent recovery and enrichment of infectious centres in the ig-positi ...19921469366
absence of genomes of dna tumor viruses and expression of oncogenes and growth factors in two esophageal carcinoma cell lines of chinese study the oncogenesis of human esophageal carcinoma, the presence of dna sequences homologous to several dna tumor viruses and the expression of oncogenes and growth factor genes were examined in two esophageal carcinoma cell lines of chinese origin, ce48t/vgh and ce81t/vgh. southern blot analyses failed to detect sequences homologous to hepatitis b virus (hbv), epstein-barr virus (ebv), herpes simplex virus type 2 (hsv-2), cytomegalovirus (cmv) or human papilloma virus (hpv) genomes. norther ...19921473373
fibrin ring granulomas in hepatitis a.fibrin ring granuloma is characterized by a fibrinous ring surrounding a central fat vacuole. it has been found in the liver and bone marrow of patients with q fever, and occasionally with visceral leishmaniasis, cytomegalovirus, epstein-barr virus, staphylococcus epidermidis infections, hodgkin's lymphoma, and hypersensitivity to allopurinol. we describe a case of serologically confirmed viral hepatitis a with this lesion in the liver biopsy. a false positive anti-hepatitis a virus igm result h ...19921473440
immunization of scid-hu mice and generation of anti-hepatitis b surface antigen-specific hybridomas by electrofusion.human hybridomas with specificity for recombinant hepatitis b surface antigen (hbsag) were produced by adoptive transfer of human peripheral blood mononuclear cells prepared from hbsag-immune donors to cb.17 mice bearing the severe combined immunodeficiency (scid) phenotype. a total of ten scid-hu mice were immunized with recombinant hbsag. eight scid-hu mice found to have human hbsag antibody in their serum were sacrificed, and single-cell suspensions were made from their spleens. the scid-hu s ...19921477301
antiproliferative effects of suramin on human cancer cells in vitro and in vivo.the present experiment was undertaken to study what types of human cancers are responsive to the antiproliferative effects of suramin. the human malignant cells used were as follows: cervical cancer (hela), mammary cancer (mcf-7), bladder cancer (ej), hepatoma (huh-7, plc/prf/5), embryonal carcinoma (pa-1), in vitro transformed fibroblasts (kmst-6, susm-1, va-13), five myeloma cell lines (kmm-1, kms-5, kms-11, kms-12, rpmi 8226), burkitt's lymphoma (raji), acute promyelocytic leukemia (hl-60), c ...19921485540
[anti-rho[d] antigen monoclonal antibodies. ii. serological characteristics of monoclonal igg1 antibodies].four igg1 monoclonal antibodies (moabs) to the rh antigen d, produced by stable epstein-barr virus transformed b-lymphoblastoid cell lines were assessed in different serological tests. their suitability in blood group typing was shown in antiglobulin, enzyme or albumin methods. the specificity and activity of moabs was tested with a panel of red cells of various rh-phenotypes. the supernatants of all four lines showed anti-d specificity and ability to react with du red cells. mean level of moabs ...19921490578
the association of c-myc rearrangements with specific types of human non-hodgkin's lymphomas.the function of c-myc in physiology is only partially known. its product has dna binding properties and plays a role in the control of proliferation and differentiation. in general, increased c-myc expression leads to proliferation and abolishment of differentiation. the involvement of c-myc in mouse plasmacytomas and human burkitt's lymphoma is well known: due to chromosomal translocation c-myc comes under the influence of regulatory elements of immunoglobulin genes, leading to increased expres ...19921493439
[transfusion risks and alternatives to transfusion].the emergence of the acquired immunodeficiency syndrome (aids) has fueled concerns of both physicians and their patients about safety of blood transfusions. although aids has generated the most fear, the risk today is extremely remote (1/60.000 units of blood). the risk of transmitting infectious disease by homologous transfusion is decreasing, as more donor screening and testing measures are implemented. the blood supply is safer that at any time, but small transfusion risks exist. the most com ...19921496180
the effect of cytokines and mitogens on the induction of c epsilon germline transcripts in a human burkitt lymphoma b cell is known to induce a 1.8 kb constant epsilon (c) transcript in human peripheral blood mononuclear cells (pbmc). this is a c germline or 'sterile' transcript which is encoded by a germline exon (i) located 5' of the epsilon switch (s) region and the four c germline exon including 3' untranslated region. among the human b cell lines examined, one burkitt lymphoma b cell line, dnd39 (epstein-barr virus negative, sigm+, cd40+), was found to respond to il-4 and express the c germline transcript. ...19921498085
oral diseases in hiv-1 oral lesions have been reported since the beginning of the aids epidemic, be they fungal, viral, bacterial, neoplastic, or non-specific in origin. the most common lesions are oral candidiasis (oc; noted in several forms) and oral hairy leukoplakia (ohl). oc appears to be directly related to levels of immunosuppression while ohl, a newly described lesion, is associated with the epstein-barr virus. although prevalence data for all types of oral lesions are scarce, this review identi ...19921499354
molecular characterization of a human anti-rh(d) antibody with a dh segment encoded by a germ-line sequence.the lambda-light-chain and lambda-heavy-chain variable-region genes of an anti-rh(d) (rh, rhesus; d, heavy-chain diversity region) human monoclonal antibody secreted by lymphocytes transformed by the epstein-barr virus have been cloned and sequenced. sequence comparison of the anti-rh(d)mab lambda-chain variable region with those of the other available human lambda chains revealed that it belonged to the human v lambda i (v lambda, variable region of lambda chain) subgroup. the greatest sequence ...19921499555
density-dependent responsiveness to autocrine growth factors of epstein-barr virus transformed human b lymphocytes.analysis of the growth requirements of epstein-barr virus (ebv)-transformed b lymphocytes shows that interleukin 1 and thioredoxin, a disulfide reducing enzyme, are able to induce a marked increase in dna synthesis in the early phases of in vitro culture. by contrast, interleukin 6 induces a steady increase in dna synthesis comparable to that observed with crude conditioned supernatant. furthermore, ebv-transformed b cells exhibit a density-dependent responsiveness to autocrine growth factors, t ...19921501588
mutagenesis by apurinic sites in normal and ataxia telangiectasia human lymphoblastoid cells.we used a shuttle vector based on the epstein-barr virus origin of plasmid replication (orip) to determine the types of mutations induced by depurination in human cells. plasmid dna was incubated at ph 2 at 40 degrees c for various times to induce up to 20 apurinic (ap) sites per 9.7-kb plasmid and electroporated into lymphoblastoid cells derived from either a normal individual or an ataxia telangiectasia patient. after replication of the vector in the human cells, plasmid dna was isolated and a ...19921503643
salivary gland dysfunction.clinically significant declines of salivary output are not a function of aging. complaints of oral dryness (xerostomia) are common in the elderly patient, however. this complaint is most often a consequence of medications; tricyclic antidepressants, certain antihypertensives, and anticholinergics can cause marked decreases in salivary flow rates. other medications such as diuretics may cause xerostomia without decreasing salivary output. systemic diseases such as sjögren's syndrome can destroy s ...19921504941
syncytial giant-cell hepatitis--a specific disease entity?syncytial giant-cell hepatitis was recently reported to be related to a paramyxovirus and carried a poor prognosis. twelve patients with syncytial giant-cell hepatitis seen in an 8 1/2-year period in our institute were reviewed. seven patients had an identifiable aetiological cause: two had autoimmune chronic active hepatitis, one had primary sclerosing cholangitis and autoimmune chronic active hepatitis, two presented with prolonged jaundice after acute hepatitis a and b, one had chronic epstei ...19921506641
viruses and the blood.haematological syndromes attributed to viruses demonstrate geographical variations in incidence and great dependence on host factors. severe haematological disease is the exception rather than the rule in dengue virus infection, and probably depends at least in part on the host immune response to the virus. the increased incidence of hepatitis-associated aplasia in east asia may reflect distribution of an infectious agent, an environmental toxin, or genetic predisposition, but probably represent ...19921511177
absence of p53 gene mutations in primary nasopharyngeal carcinomas.alterations in the p53 tumor suppressor gene and epstein-barr virus status were investigated in 15 nasopharyngeal carcinoma (npc) biopsies, 4 xenografts, and 2 cell lines from the cantonese region of southern china. one other established npc cell line obtained from a northern chinese patient was also studied. restriction fragment length polymorphism analysis revealed a loss of heterozygosity for chromosome 17p, where the p53 gene resides, in only one of 15 npc biopsies. polymerase chain reaction ...19921511442
no evidence for linkage of familial hypertrophic cardiomyopathy and chromosome 14q1 locus d14s26 in a chinese family: evidence for genetic understand the molecular basis of familial hypertrophic cardiomyopathy (fhc) in the chinese population, a family with fhc was investigated. nineteen family members who were 16 years of age or older were examined by m-mode or two-dimensional echocardiography. eight members were diagnosed to be affected echocardiographically or clinically. lymphocytes isolated from 20 family members were successfully transformed into permanent lymphoblastoid cell lines by epstein-barr virus. three genomic dna p ...19921511975
familial nasopharyngeal carcinoma in patients who are not chinese.nasopharyngeal carcinoma (npc) is a malignancy that is prominent in cantonese chinese people. it is presumed to result from an interaction of genetic and environmental factors, including the epstein-barr virus (ebv). in an attempt to further clarify the pathogenesis of this disease, an evaluation of npc occurring in racial/ethnic groups not considered susceptible to this disease could be informative.19921515979
hla class i and ii molecules present influenza virus antigens with different kinetics.human leukocyte antigen (hla) class i and class ii molecules differ with respect to their intracellular pathways and the compartments where they associate with processed antigen. to study possible consequences of these differences for the kinetics of antigen presentation by hla class i and class ii molecules, we analyzed changes in the concentrations of free intracellular calcium ions in influenza virus-specific t cell clones after recognition of specific antigen/hla complexes. hla class ii-rest ...19921516624
unusual oral presentation of non-hodgkin's lymphoma in association with hiv 4.4% of human immunodeficiency virus-associated non-hodgkin's lymphoma the presenting lesion is seen in the mouth. often the lesion may clinically resemble a less sinister process, and a definitive diagnosis of lymphoma may be delayed. we describe three unusual cases of non-hodgkin's lymphoma, appearing intraorally in association with other oral lesions, in hiv-positive homosexual men. the three patients reported here were all diagnosed as having diffuse, large-cell malignant non-hodgkin's ly ...19921518649
the role of histopathology in hepatic hepatic transplantation, the recipient and the graft must manage a difficult symbiosis. the causes that can unbalance the mutual adaptation are various, but the clinical-biochemical hepatic graft syndromes they produce are not specific. morphological study of the graft shows a distinct pattern for each type of dysfunction etiopathogeny. such study may find: (1) immune attack: acute rejection or chronic rejection; (2) technical complications in the biliary tract or in the blood perfusion of th ...19921523358
application and comparison of silver intensification methods for the diaminobenzidine and diaminobenzidine-nickel endproduct of the peroxidation reaction in immunohistochemistry and in situ hybridization.silver-intensification methods described in the literature for the diaminobenzidine (dab) and diaminobenzidine-nickel (dab/ni) endproduct of the peroxidase reaction were compared in model systems after immunoperoxidase and in situ hybridization. first, these methods were compared in immunohistochemical model systems, using the demonstration of glial fibrillar acidic protein (gfap) and prostate-specific antigen (psa) in paraffin sections of human brain and prostate tissue, respectively. when dab ...19921532404
nasopharyngeal carcinoma: diagnosis, staging, and management.nasopharyngeal carcinoma is a common tumor in several parts of the world, accounting for approximately 18% of cancers in chinese populations, but is an uncommon tumor in whites. genetic, environmental, and viral factors all seem to have a part in the genesis. its various forms have been classified by the world health organization (who) into three histologic categories, all of which can be identified as squamous cell carcinomas by electron microscopy. the who types 2 and 3 tumors are associated w ...19921532503
a human b-cell lymphoma line with a de novo multidrug resistance phenotype.a human diffuse large cell lymphoma line (wsu-dlcl) expressing multidrug resistance (mdr) was established from a patient with primary chemotherapy-resistant disease. this cell line has the same phenotypic features as malignant cells from the patient. the established cell line has features of a mature b-cell neoplasm with no evidence for commitment to other lineages. wsu-dlcl grows in suspension forming relatively large clumps of cells with a doubling time of 20 hours. by light microscopic examin ...19921540884
the human repertoire of antibody specificities against thomsen-friedenreich and tn-carcinoma-associated antigens as defined by human monoclonal antibodies.human monoclonal antibodies specific for tumour-associated thomsen-friedenreich (tf) [gal(beta 1-3)galnac(alpha)-o-] and tn [galnac(alpha)-o-] glycoproteins were prepared using peripheral blood lymphocytes from healthy blood donors. the b lymphocytes were either directly transformed with epstein-barr virus (ebv) or transformed after an in vitro stimulation period with synthetic glycoproteins. the ebv-transformed lymphocytes were subsequently fused with a mouse-human heteromyeloma to secure antib ...19921540975
generation of human monoclonal antibodies that confer protection against pertussis toxin.a panel of human monoclonal antibodies reactive with pertussis toxin has been generated by means of epstein-barr virus infection. one of these, the 3f11 monoclonal antibody, showed the ability to neutralize in vitro and in vivo the toxic effects of the toxin. western blot (immunoblot) analysis located the 3f11 epitope on the s3 subunit.19921541543
lymphocyte transformation and thiol compounds; the role of adf/thioredoxin as an endogenous reducing agent.adf (adult t-cell leukemia-derived factor), an inducer of il-2r with growth promoting activity, is a homologue of thioredoxin which is involved in many thiol-dependent reducing reactions. adf is constitutively produced and released by human lymphoid cell lines transformed by lymphocyte-tropic viruses, such as human t-lymphotropic virus type i (htlv-i) and epstein-barr virus (ebv). we found that the viability and growth of these adf high-producer cell lines (atl-2, hut102, mt-2, 3b6 and rpm18866) ...19921542302
chronic fatigue syndrome and women: can therapy help?this article presents current research on chronic fatigue syndrome, which currently afflicts mostly females between the ages of 25 and 55. because depression is a common symptom of chronic fatigue syndrome, mental health practitioners are often involved with the victims and must formulate an appropriate treatment strategy that considers the physiological, intrapsychic, interpersonal, and environmental aspects of the client. this article includes case material focusing on a woman who was medicall ...19921542805
large granular lymphocyte proliferation with the natural killer-cell phenotype.lymphoproliferated disorders involving large granular lymphocytes (lgl) can be divided into a common t-cell subset (cd3+, cd8+) and a rarer natural killer (nk)-cell subset (cd2+, cd3-). the immunophenotype, clinical pathologic features, and cytogenetic and molecular genetic analyses are reported for seven patients with nk-cell-lgl proliferation. the typical immunophenotype was cd2+, cd3-, cd4-, cd11b+, and cd16+ or cd56+. a low but variable percentage of cells were cd8+ or cd57+. unusual phenoty ...19921543158
thymic pseudotumorous enlargement due to follicular hyperplasia in a human immunodeficiency virus sero-positive patient. immunohistochemical and molecular biological study of viral infected enlargement of the thymus suggesting a tumor was discovered in a 28-year-old man who had early-stage acquired immune deficiency syndrome. a biopsy was performed. the adipose involuted thymus, with persistence of many hassall's corpuscles, was judged to be a large lymphoid follicular hyperplasia. this follicular hyperplasia was similar to that previously described for lymph nodes, spleen, and other lymphoid tissues at earlier stages of human immunodeficiency virus infection, before the develop ...19921543167
a 41-kilodalton protein is a potential substrate for the p210bcr-abl protein-tyrosine kinase in chronic myelogenous leukemia cells.chronic myelogenous leukemia (cml) is characterized by a translocation involving the c-abl protein-tyrosine kinase gene. a chimeric mrna is formed containing sequences from a chromosome 22 gene (bcr) at its 5' end and all but the variable exon 1 of c-abl sequence. the product of this mrna, p210bcr-abl, has constitutively high protein-tyrosine kinase activity. we examined k562 cells and other lines established from cml patients for the presence of phosphotyrosine (p-tyr)-containing proteins which ...19921545812
adhesion of t and b lymphocytes to extracellular matrix and endothelial cells can be regulated through the beta subunit of vla.investigating the regulation of very late antigen (vla)-mediated functions, we found that ts2/16, a mab directed against the beta chain of the vla group of integrins, can induce binding of resting peripheral blood lymphocytes, cloned t lymphocytes, and epstein barr virus-transformed b cells to extracellular matrix components, fibronectin, laminin, and collagen, but not to fibrinogen. the antibody stimulates vla-4-, vla-5-, and vla-6-mediated binding. furthermore, it induces vla-4-mediated bindin ...19921560035
report on the x-linked lymphoproliferative disease in an australian family.x-linked lymphoproliferative disease is characterized by immune deficiency, particularly to the epstein-barr virus and by a tendency to develop fatal infectious mononucleosis, acquired hypogammaglobulinaemia or malignant lymphoma. this disorder has been diagnosed in three boys, two brothers and a maternally related cousin, residing in australia. the proband presented at 6 years of age with fulminating infectious mononucleosis. his 9 year old male cousin had developed an ileal burkitt lymphoma on ...19921562373
antiproliferative effect of antilymphocyte globulins on b cells and b-cell lines.antithymocyte and antilymphocyte globulins (alg) are currently used as immunosuppressive agents in organ transplantation and for the treatment of acute graft-versus-host disease and aplastic anemia. since any type of immunosuppressive treatment is known to carry the risk of developing b-cell lymphoproliferative disorders, we investigated the in vitro effect of alg on human b-cell activation and proliferation. the data demonstrate that whatever the source of lymphocytes used for alg preparation ( ...19921562743
epstein-barr virus-associated lymphoma in a patient with rheumatoid arthritis treated with cyclosporine. 19921565156
cell type-specific expression and negative regulation by retinoic acid of the human n-myc promoter in neuroblastoma cells.expression of the n-myc oncogene is an important determinant of tumor behavior in human neuroblastoma. to study the regulation of n-myc, we have subcloned fragments of the 5' flanking region of the human n-myc gene upstream of the chloramphenicol acetyl transferase (cat) reporter gene, and assayed for promoter activity in transient transfections into neuroblastoma and other cell lines. upstream sequences were found to possess promoter activity to within 121 bp of the major cap site (-121). negat ...19921565467
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