PMID(sorted ascending)
[the role of epstein-barr viruses as etiologic agents in the mononucleosis syndrome].mononucleosis syndrome represents a number of symptoms with different etiology and pathogenicity with similar clinical features. the aim of the study was to investigate etiologic structure of mononucleosis syndrome, effects of etiologic factors and age on the severity of clinical features, clinical forms of the disease, complications and the outcome. the investigation was conducted in 46 patients treated at the clinic who had been diagnosed as having mononucleosis syndrome. ebv-im was confirmed ...19921344437
lymphoid interstitial pneumonia not associated with hiv infection: role of epstein-barr virus.the polymerase chain reaction (pcr) method was used to determine the presence of epstein-barr virus sequences in seven cases of pulmonary lymphoid interstitial pneumonia not associated with hiv infection. evidence of epstein-barr virus genome was found in three of six cases in which beta-globin gene, used as an internal control for the integrity and amplifiability of the tissue dna, could be detected. these results suggest that epstein-barr virus infection is not always required for the developm ...19921344810
characterization of the topography of epstein-barr virus infection in human immunodeficiency virus-associated lymphoid tissues.a highly sensitive in situ hybridization methodology for epstein-barr virus (ebv) rna was used to determine the topography of ebv infection in 16 cases of human immunodeficiency virus-associated lymphoid tissues. four lymphomas, 11 persistent generalized lymphadenopathy (pgl) lymph nodes, and one lymphoepithelial cyst were studied. the pattern of ebv infection was diffuse in all lymphoma cases and predominantly interfollicular in pgl nodes. no discernable pattern of infection was present in the ...19921344820
heart transplantation in children: mid-term results and quality of life.from 1987 to 1991, heart transplantation was undertaken in 49 infants and children with either end-stage cardiomyopathies (28 patients) or severe congenital heart disease (21 patients including 16 having already been surgically but unsuccessfully treated). their age ranged from 13 days to 15 years (mean = 4.5 +/- 4.2 years; median = 2.5 years). there were 12 early and 7 late deaths (overall mortality = 38%), mainly due to graft dysfunction, acute or chronic rejection, and infectious complication ...19921345106
[tubulo-interstitial nephritis and uveitis in a 13-year old boy]. 19921345295
viral infection in the renal transplant recipient.the viral infections with greatest impact on the renal transplant recipient are those due to cytomegalovirus, epstein-barr virus, and the two hepatitis viruses, hepatitis b and c. all of these are modulated by the administered immunosuppressive therapy, and all have both direct and indirect effects on the transplant patient. the direct effects are the infectious disease clinical syndromes that are produced (fever and malaise, pneumonia, hepatitis, and so forth). the indirect effects are several- ...19921345623
human mature t cells that are anergic in vivo prevail in scid mice reconstituted with human peripheral these studies we have characterized the human cells that repopulate severe combined immunodeficient (scid) mice after injection of adult peripheral blood or cord blood (hu-pbl-scid mice). in all organs of the chimeras, and at any time point tested, single-positive (cd4+ or cd8+) t cells that expressed the alpha/beta t cell receptor (tcr) prevailed. all t cells were cd45ro+ and the majority were also hla-dr+. thus, the human t cells in the chimeras exhibited the phenotype of mature, memory cel ...19921346272
lymphocytic sialadenitis of sjögren's syndrome associated with chronic hepatitis c virus liver disease.viral infection has often been suggested as a possible cause of sjögren's syndrome or chronic lymphocytic sialadenitis, and epstein-barr virus has been found in the salivary glands of patients with this condition. after we had noted sjögren's syndrome in several patients infected with hepatitis c virus (hcv), a virus also excreted in saliva, we set up a prospective study to investigate the association of chronic lymphocytic sialadenitis, with or without symptoms, to chronic hcv liver disease. th ...19921346409
genetic cause of leukocyte adhesion molecule deficiency. abnormal splicing and a missense mutation in a conserved region of cd18 impair cell surface expression of beta 2 integrins.patients with leukocyte adhesion molecule (cd11/cd18, beta 2 integrins) deficiency have structural defects in the common beta subunit (cd18), which prevent heterodimer formation and normal cell surface expression of these receptors, leading to life-threatening bacterial infections. to elucidate the nature of these defects in a patient with partial (type ii) deficiency, abnormal cd18 cdna clones were isolated, using the polymerase chain reaction to amplify the patient's b cell-derived cdnas. sequ ...19921346613
euphorbia species. 19921346699
immune surveillance, organophosphorus exposure, and lymphomagenesis.prevalence of lymphoproliferative disorders is increased in populations with various chemical exposures, including organophosphorus compounds. lymphomas are also more common in individuals with a substantially decreased monocyte esterase activity. organophosphorus compounds inhibit esterases associated with monocytes, natural killer (nk) cells, lymphokine-activated killer (lak) cells, and cytotoxic t lymphocytes, and these inhibitory effects impair immune surveillance and cytotoxic functions med ...19921346889
demonstration of passenger leukocytes in a case of epstein-barr virus posttransplant lymphoproliferative disorder using restriction fragment length polymorphism analysis.lymphocytic populations of the posttransplant lymphoproliferative disorder in a completely matched renal allograft and a recipient's regional lymph node were examined, using restriction fragment length polymorphism analysis with probe ynh24 to the variable number tandem repeat d2s44. the donor's dna was found in lymphocytes extracted from both the grafted kidney and the recipient's lymph node 26 days after engraftment. in both these organs, the results of in situ hybridization with a terminally ...19921346957
engagement of major histocompatibility complex class i and class ii molecules up-regulates intercellular adhesion of human b cells via a cd11/cd18-independent mechanism.we have studied the role of major histocompatibility complex (mhc) molecules in the regulation of intercellular adhesion of human b cells. we found that molecules able to bind to mhc class ii molecules, such as monoclonal antibodies or staphylococcal enterotoxins, induced rapid and sustained homotypic adhesion of epstein-barr virus (ebv)-transformed b cell lines as well as peripheral blood b lymphocytes. moreover, anti-mhc class i monoclonal antibodies also stimulated intercellular adherence. ad ...19921347012
regulation of germ-line epsilon transcription and induction of epsilon switching in cloned ebv-transformed and malignant human b cell lines by cytokines and cd4+ t induces germ-line epsilon transcript synthesis in normal human b cells, but a second signal provided by cd4+ t cells is required for subsequent production of productive epsilon mrna and ige synthesis. in the present study we demonstrate that il-4 specifically induces germ-line epsilon transcripts in most ebv lymphoblastoid (lcl) and ebv+ and ebv- burkitt's lymphoma (bl) cells without inducing ige synthesis. however, cocultivation of cloned sigm+, sige- ebv-lcl or bl cells with activated cd4 ...19921347554
human hematopoietic cells and thymic epithelial cells induce tolerance via different mechanisms in the scid-hu mouse study the role of thymic education on the development of the human t cell repertoire, scid-hu mice were constructed with fetal liver and fetal thymus obtained from the same or two different donors. these animals were studied between 7 and 12 mo after transplantation, at which times all thymocytes and peripheral t cells were derived from stem cells of the fetal liver graft. immunohistology of the thymus grafts demonstrated that thymic epithelial cells were of fetal thymus donor (ftd) origin. d ...19921348080
specific lysis of targets expressing varicella-zoster virus gpi or gpiv by cd4+ human t-cell clones.varicella-zoster virus (vzv)-specific cd4-positive t cells are known to lyse targets expressing vzv antigen, but little is known of the glycoprotein specificity or phenotype of these cells. to test the ability of t cells to distinguish between gpi and gpiv (which share an antibody-defined epitope), we prepared clones from blood from four healthy individuals by limiting dilution. among 68 t-cell clones from four donors which were vzv specific in tests of proliferation, 30 lysed autologous epstein ...19921348545
inhibition of molt-4-endothelial adherence by synthetic peptides from the sequence of icam-1.previous studies have shown that inflammatory pathologies are mediated by lymphocyte adhesion to endothelium and subsequent transmigration through the endothelial monolayer. lymphocyte-endothelial adherence is, in part, caused by the leukocyte integrin lfa-1 binding to icam-1, its ligand on endothelial cells. synthetic peptides based on specific amino acid sequences of human icam-1 inhibit the adherence of a lymphocytic cell line, molt-4, to cytokine-stimulated endothelial cells. a total of 26 p ...19921349017
antibodies to epstein-barr virus and cytomegalovirus in relation to cd4 cell number in human immunodeficiency virus 1 infection.interaction between herpesviruses and human immunodeficiency virus (hiv)1 is postulated in the progression of hiv disease. in order to evaluate the specific antibody responses directed to epstein-barr virus (ebv) and cytomegalovirus (cmv) and to provide serological evidence suggesting reactivation of these viruses able to accelerate the immunodeficiency, we studied iga and igg titres to ebv and cmv in the serum of hiv positive patients in relation to the cd4 cell number. the titres of igg antibo ...19921349340
membranes of activated cd4+ t cells expressing t cell receptor (tcr) alpha beta or tcr gamma delta induce ige synthesis by human b cells in the presence of the present study it is demonstrated that human b cells can be induced to switch to ige production following a contact-mediated signal provided by activated t cell receptor (tcr) gamma delta+, cd4+ and tcr alpha beta+, cd4+ t cell clones and interleukin (il)-4. the signal provided by these t cell clones was antigen nonspecific, indicating that the tcr alpha beta/cd3 or tcr gamma delta/cd3 complexes were not involved in these t-b cell interactions. activated tcr alpha beta+, cd8+, and tcr gamm ...19921349531
alterations of the p53 gene in nasopharyngeal carcinoma.nasopharyngeal carcinoma (npc) is a malignancy which is consistently associated with the epstein-barr virus (ebv). the structure of the ebv genome in npc suggests that npc is a clonal proliferation of epithelial cells which emerges after ebv infection. the disease develops with high incidence in specific populations in discrete geographic locations, implicating possible genetic or environmental cofactors. mutations of the p53 gene are among the most frequent genetic changes found in a large vari ...19921349927
il2- and il4-dependent proliferation of t-cell clones derived early after allogeneic bone marrow transplantation: studies of patients with chronic myelogenous an attempt to explore t-cell functions shortly after allogeneic bone marrow transplantation more fully, il2- and il4-dependent proliferation was assessed on cd4+ tcr alpha beta+ t-cell clones derived 4-6 weeks after transplantation. both allogeneic pooled peripheral blood mononuclear cells and epstein-barr virus-transformed b-cell lines (bcl) could function as accessory cells (ac) for pha activation of t-cell clones. although minimal clonal proliferation was seen when the t-cell activation si ...19921350537
homozygous hereditary c3 deficiency due to a partial gene deletion.the molecular mechanism of c3 deficiency in an afrikaans patient with recurrent pyogenic infections was studied. restriction enzyme analysis showed a gene deletion of 800 base pairs (bp) mapping to the alpha chain of c3. amplification of genomic dna, using the pcr, demonstrated that the deletion included exons 22 and 23 of the c3 gene. truncated mrna was shown in an epstein-barr virus-transformed b-cell line by pcr amplification of first-strand cdna. a consequence of this deletion was that the r ...19921350678
the epstein-barr virus encoded membrane protein (lmp) induces phenotypic changes in epithelial cells.the epstein-barr virus (ebv)-encoded membrane protein, lmp, is expressed in a proportion of undifferentiated nasopharyngeal carcinomas (npc). previous studies have shown that the transfection of the gene encoding lmp into a human keratinocyte line, rhek-1, induces morphological alterations and a reduced expression of cytokeratins. we have analyzed immunophenotypic changes in the rhek-1 line following lmp-transfection and compared these changes with the phenotype of npc biopsies. we demonstrate a ...19921352076
antigen-independent adhesion of cd45ra (naive) and cd45ro (memory) cd4 t cells to b cells.we found that naive (cd45ra+) cd4 t cells have a lower capacity of adhesion to epstein-barr virus (ebv) immortalized b cells than memory (cd45ro+) cd4 t cells, as judged by conjugate formation. this would appear to be due to differences in the expression of adhesion molecules [lymphocyte function-associated antigen (lfa)-1, cd2]. however, kinetic studies showed that the degree of adhesion of naive t cells to b cells was stable over 60 min while that of memory t cells, like that of unseparated cd ...19921352461
transient expression of the epstein-barr virus lmp1 gene in human primary b cells induces cellular activation and dna synthesis.the epstein-barr virus (ebv)-encoded latent membrane protein 1 (lmp1) and epstein-barr virus nuclear antigen 2 (ebna2) are expressed in ebv-immortalized human b cells. it has previously been shown that transfection of the lmp1 and ebna2 genes into burkitt's lymphoma cell lines results in the up-regulation of cd23, cd21, icam-1 and lfa-1 cell-surface proteins. in the present study, the effects of transient expression of the lmp1 and ebna2 genes were studied in normal primary human b cells pretrea ...19921354347
carrier detection in agammaglobulinemia by x chromosome inactivation analysis.using a recently developed strategy to analyze patterns of x chromosome inactivation in cell populations, we found that two mothers and a sister were carriers in three atypical or sporadic cases of patients with agammaglobulinemia, two of whom were brothers. in this study, a phosphoglycerate kinase 1 (pgk1) gene probe was used to detect patterns of methylation of x-chromosome genes. a random pattern of x inactivation was observed in isolated peripheral blood granulocytes. in contrast, one of the ...19921354911
effect of inhaled corticosteroids on ebv-specific cytotoxic t cells and ebv oropharyngeal excretion. 19921355172
ebv strain variation: geographical distribution and relation to disease state.the strains of epstein-barr virus (ebv) were characterized in epithelial and lymphoid malignancies from geographic regions with high or low incidence. the predominant strains in nasopharyngeal carcinoma (npc) from regions with elevated incidence were ebv type 1 in southeast asia and mediterranean africa. in alaskan eskimos, a distinct variant of ebv type 2 was found in npc and carcinoma of the parotid gland. this strain contained polymorphisms characteristic of the asian ebv type 1. the strains ...19921356286
production of natural killer cell stimulatory factor (interleukin 12) by peripheral blood mononuclear cells.natural killer cell stimulatory factor (nksf), or interleukin 12 (il-12), is a 70-kd heterodimeric cytokine composed of two covalently linked chains, p40 and p35. nksf/il-12 has multiple effects on t and nk cells and was originally identified and purified from the supernatant fluid of epstein-barr virus (ebv)-transformed human b lymphoblastoid cell lines. we have produced a panel of monoclonal antibodies against both chains of nksf/il-12. some of these antibodies have neutralizing activity, and ...19921357073
role of the production of natural killer cell stimulatory factor (nksf/il-12) in the ability of b cell lines to stimulate t and nk cell proliferation.we have previously used human epstein barr virus (ebv)-transformed b lymphoblastoid cell lines for the identification and purification of a novel cytokine, natural killer cell stimulatory factor (nksf/il-12), that has pleiotropic effects on human lymphocytes. b cell lines are also routinely employed as feeder cells for the culture of t and natural killer (nk) cells. in this report we describe the ability of two nksf/il-12 producing b cell lines (rpmi-8866 and cess) and two nonproducing lines (ra ...19921358460
malignant histiocytosis in a patient with chronic active epstein-barr virus infection. 19921359251
peritoneal sanctuary for human lymphopoiesis in scid mice injected with human peripheral blood lymphocytes from epstein-barr virus-negative donors.the successful engraftment in scid mice of intraperitoneally (i.p.) injected human lymphocytes (hu-pbl-scid) and the failure of intravenously injected peripheral blood lymphocytes (pbl) directed the present study to investigate the early events of donor cell proliferation in the peritoneal cavity. we found focal lymphocyte engraftment together with histio-monocytic interleukin (il)-6+ cell phenotypes which must have been transferred with the human cell inoculum, which could explain certain immun ...19921359971
[polymerase chain reaction (pcr) in microbiology. value and practical applications].the gene amplification reaction (pcr) developed in 1985 is a molecular biology instrument which is used to amplify genomic restriction fragment lengths of micro-organisms found in biological samples. at the moment, its applications to direct microbiological diagnosis remain to be determined precisely, and its strict technique is awaiting standardization. however, it is a powerful tool for molecular typing used to follow up an antiviral therapy or to analyse retrospectively paraffin-embedded tiss ...19921360661
epstein-barr virus-induced transformation of human b lymphocytes: the effect of l-leucyl-l-leucine methyl ester on inhibitory t cell populations.epstein-barr virus-mediated transformation of human b lymphocytes is inhibited by human t lymphocytes as well as by interferon-gamma. removal of the inhibitory cell populations is essential in order to achieve successful transformation in vitro. cells with the capacity to inhibit outgrowth of lymphoblastoid cell lines can be removed by pretreatment of peripheral blood mononuclear cells with l-leucyl-l-leucine methyl ester. this treatment eliminates monocytes, nk-cells and a cd8+ t cell subpopula ...19921362566
the pathogenesis of aids lymphomas: a foundation for addressing the challenges of therapy and prevention.the association between aids and a spectrum of malignancies relates to chronic, profound defects in both cellular and humoral mechanisms of immune surveillance. ironically, as aids patients live longer in response to increasingly effective antiretroviral therapies, the incidence of aids-related malignancies will continue to rise. the emergence of non-hodgkin's lymphomas (nhl) as a major sequela of hiv infection bears a striking relationship to depletion of cd4 lymphocytes, particularly below 50/ ...19921362682
occurrence of nasopharyngeal carcinoma in aboriginals of taiwan: report of 14 cases.the authors describe the rare occurrence of nasopharyngeal carcinoma (npc) in 14 aboriginals of taiwan (abt), a minor ethnic group now accounting for less than 2% of the total population in taiwan. the observation is epidemiologically unusual, representing a low-risk ethnic group in an npc prevalent area. with regard to patient characteristics, symptomatology and pathology, we have not found any appreciable differences in reports from other geographic areas. serological profiles of antiebv-vca ( ...19921363202
[recent advances in the etiopathology of chronic b-lymphocytic leukemia].considerations to multistep development of b-lymphocytic chronic leukaemia are presented under the following aspects: experimental in vitro cultures of bone marrows b lymphocyte precursors, polyclonal nature of proliferating b lymphocytes in the early stage of cll, especially in the autoimmune status, the role of viruses, the effect of nonrandom chromosome abnormalities and associated alterations in gene functions, clonal evolution during terminal stages of cll.19921363442
natural killer cell stimulatory factor (nksf) or interleukin-12 is a key regulator of immune response and inflammation.natural killer cell stimulatory factor (nksf) or interleukin-12 (il-12) is a heterodimeric cytokine of 70 kda formed by a heavy chain of 40 kda (p40) and a light chain of 35 kda (p35). although it was originally identified and purified from the supernatant of epstein-barr virus-transformed b cell lines, it has been shown that among peripheral blood cells nksf/il-12 is predominantly produced by monocytes, with lower production by b cells and other accessory cells. the most powerful inducers of nk ...19921364096
epstein-barr virus infection in nasopharyngeal carcinoma.nasopharyngeal carcinoma develops with extremely high incidence in discrete populations and geographic locations. it is consistently associated with the ubiquitous epstein-barr herpesvirus (ebv). the structure of ebv dna indicates that the tumor is a clonal proliferation that developed subsequent to ebv infection. specific viral genes are consistently expressed within all cells of the tumor. this consistent expression may indicate that the viral gene products are required for tumor growth. genet ...19921365543
expression of the epstein-barr virus encoded latent membrane protein in tumor cells of hodgkin's disease occurring in childhood.paraffin sections from 21 cases of hodgkin's disease (hd), 28 cases of non-hodgkin's lymphomas (nhl) and 34 cases of non-specific reactive lymphadenitides occurring in childhood were examined for the presence of the epstein-barr virus (ebv)-encoded latent membrane protein (lmp) using a double layer immunohistochemical method. lmp was detected in 12/21 (57%) cases of hd but not in nhl or reactive lymph nodes. lmp reactivity was restricted to reed-sternberg and hodgkin's (hrs) cells in 4 of 9 (45% ...19921365766
a human-human hybridoma secreting anti-pseudomonas aeruginosa exotoxin-a monoclonal antibody with highly potent neutralizing activity.a hybridoma secreting human monoclonal antibody (mab) against pseudomonas aeruginosa exotoxin a (pea) was constructed by fusing epstein-barr virus-transformed peripheral blood lymphocytes with human b lymphoblastoid cell line taw-925. the human-human hybridoma stably produced human igg2 mab at the rate of 0.4-0.5 microgram/ml per 10(6) cells per day for more than six months, and the mab was capable of neutralizing the in vitro cytotoxic and in vivo lethal effects of pea with approximately 100- a ...19901369271
production of human tissue-type plasminogen activator in different mammalian cell lines using an epstein-barr virus vector.human tissue-type plasminogen activator (t-pa) cdna inserted into an epstein-barr virus (ebv) derived expression vector was transfected into human hela, 293, k-562 and hamster cho-k1 cells and the expression of t-pa was studied. the best t-pa producing cell clones were found among cho-k1 cells (up to 11 micrograms d-1 per 10(6) cells). however, hela and 293 cells were most efficiently transfected, e.g. about 70% of the selected cell clones were t-pa positive. the vector dna copy numbers correlat ...19901369273
a human hybrid hybridoma producing a bispecific monoclonal antibody that can target tumor cells for attack by pseudomonas aeruginosa exotoxin fusing a human hybridoma producing an igg2 kappa antibody against human a431 epidermoid carcinoma cells with an epstein-barr virus-transformed human b lymphocyte producing an igg2 kappa antibody against pseudomonas aeruginosa exotoxin a, we established a hybrid hybridoma producing a bispecific monoclonal antibody reacting with both a431 cells and the exotoxin. human igg was purified from the culture supernatant of the hybrid hybridoma, and the bispecific monoclonal antibody in the igg prepara ...19901369279
expression of recombinant calf prochymosin in mammalian cell culture.the calf preprochymosin cdna was cloned into an extrachromosomal mammalian cell expression vector containing epstein-barr virus sequences using polymerase chain reaction. transfection of hela cells yielded hygromycin b resistant cell clones, expressing immunoreactive prochymosin, which was quantitatively secreted into the culture medium. based on western blotting we estimated that selected cell clones produced about 10-20 mg prochymosin per liter in 20 h. the biological activity of the secreted ...19911369334
factor(s) required by ebv transformed lymphocytes to grow under limiting dilution was demonstrated to promote growth of ebv transformed lymphocytes. however il-6 was ineffective at promoting growth of ebv transformed lymphocytes cultured at the single cell level under limiting dilution conditions. on the contrary, hecs, which is known to contain il-6, supported very efficiently the growth of 1-2 ebv transformed cells. when il-6 was removed from hecs, by using specific antibodies, no reduction in hecs activity was observed, indicating that probably more than one growth fa ...19911369369
possible anti-tumor promoting properties of marine algae and in vivo activity of wakame seaweed extract. 19921369377
chalcone tetramers, lophirachalcone and alatachalcone, from lophira alata as possible anti-tumor promoters.two chalcone tetramers were isolated as inhibitors of epstein-barr virus (ebv)-activation induced by a tumor promoter, teleocidin b-4, from a medicinal plant in tropical west africa, lophira alata (ochnaceae). one of them was identified as lophirachalcone. the other, named alatachalcone, was new, and the structure was determined by spectral properties. both compounds also showed potent inhibitory activities against teleocidin b-4-induced inflammation on mouse ear. in an initiation-promotion expe ...19921369383
multilocus dna fingerprint analysis of cell banks: stability studies and culture identification in human b-lymphoblastoid and mammalian cell lines.the technique of multilocus dna fingerprinting has great potential for the authentication of animal cell cultures and in identification of cross-contamination. the alec jeffreys probes 33.6 and 33.15 were used as multilocus probes to demonstrate the consistent dna fingerprint profiles in human peripheral blood and its derivative epstein-barr virus (ebv) transformed b-lymphoblastoid cultures maintained by repeated subculture for six months. however, fingerprint analysis of ebv transformed culture ...19921369387
clonal epstein-barr virus in lymphoepithelioma-like carcinoma of the stomach: demonstration of viral genome by in situ hybridization and southern blot analysis.lymphoepithelioma (le), originally described in the nasopharynx, is an undifferentiated carcinoma with heavy lymphocytic infiltrate. the tumor is common in southeast asia, particularly in southern china, where the epstein-barr virus (ebv) association has been documented more consistently than in western countries. tumors histologically similar to le have been described also in other anatomical sites, mostly of fore-gut origin, such as salivary gland, tonsil, lung, thymus, and more recently stoma ...19921369803
methylation of discrete sites within the enhancer region regulates the activity of the epstein-barr virus bamhi w promoter in burkitt lymphoma lines.eight of the nine viral antigens known to be expressed in in vitro epstein-barr virus (ebv)-transformed b-lymphoblastoid cell lines are downregulated in ebv-carrying burkitt lymphomas (bl). only ebna1 can be detected in bl biopsies and bl-derived cell lines that maintain the representative phenotype during culture in vitro (group i bl lines). this restricted pattern of viral gene expression is accompanied by extensive ebv dna methylation and can be reversed by treatment with the demethylating ag ...19921370095
expression of cd45 isoforms by epstein-barr virus-transformed human b lymphocytes.cd45 is the most common protein tyrosine phosphatase (ptpase) in the membrane of white blood cells, serving as a potent regulator of lymphocyte activation and signal transduction. while the amino acid sequence of the intracellular domain of the molecule is conserved, that of the extracellular domain occurs in multiple isoforms, each of the result of alternative mrna splicing. in t lymphocytes, the lowest relative molecular mass (mr) form, cd45ro, is associated with acquisition of memory function ...19921370260
regulation of b-cell growth and immunoglobulin gene transcription by interleukin-6.interleukin-6 (il-6) stimulates growth and immunoglobulin (lg) secretion in epstein-barr virus (ebv)-infected b cells. in this study, we demonstrate that b-cell activation by il-6 is associated with an initial induction of c-myc, a gene believed to act as a competence factor for increased rna transcription and dna replication, and by increases in dna, rna, and protein synthesis, as well as cell number. il-6 increased the levels of lg mrna per cell in comparison to a non-cycle-dependent cellular ...19921370387
identification of a cd21 receptor-deficient, non-ig-secreting peripheral b lymphocyte subset in hiv-seropositive drug abusers.we have studied 61 hiv-seropositive heroin addicts (18 asymptomatic, 20 arc, and 23 aids cases), 26 hiv-seronegative heroin addicts, and 45 healthy blood donors, matching the groups each other for age and sex. we have focused on the phenotypic characteristics of b subpopulations in the peripheral blood of hiv-seropositive and -seronegative drug abusers, paying particular attention to the consistence of the "cd20+" b cell subset, which poorly expresses the cd21 membrane receptor for the c3d and e ...19921370396
sequence variation of cytotoxic t cell epitopes in different isolates of epstein-barr virus.previous results have identified two distinct cytotoxic t lymphocyte (ctl) epitopes encoded by epstein-barr virus (ebv), teta (orf blrf3/berf1 residues 329-353) and eenl (orf berf3/berf4 residues 290-309). measurement of the specificities of ctl clones (teta-specific clone 13 and eenl-specific clone 7) directed against these epitopes indicated that the eenl epitope is conserved in all strains of ebv tested while the teta epitope varied between individual virus strains. sequencing of the dna regi ...19921370413
the specificity of recognition of a cytotoxic t lymphocyte epstein-barr virus (ebv)-specific cd8+ cytotoxic t lymphocyte (ctl) clone (lc13) was shown to recognize the minimal peptide determinant flrgraygl from the ebna 3 antigen of the bl74 strain of ebv. the equivalent epitope from the b95-8 strain (flrgraygi) is not recognized when endogenously presented and the peptide is 15-fold less active than flrgraygl. a replacement set of peptides was synthesized in which each residue within flrgraygl was sequentially replaced with all other genetically code ...19921370414
molecular analysis of the induction of immunoglobulin e synthesis in human b cells by interleukin 4 and engagement of cd40 antigen.the molecular events leading to immunoglobulin e (ige) synthesis in human sige- b cells stimulated with interleukin 4 (il-4) and anti-cd40 monoclonal antibody (mab) 626.1 were analyzed. anti-cd40 mab increased the levels of il-4-induced germline c epsilon transcripts and induced the production of mature c epsilon mrna. these effects were dependent on the presence of il-4. nested primer pcr revealed deletional switch recombination occurring only in b cell stimulated with both il-4 and anti-cd40 m ...19921370532
mapping of b-cell epitopes on the polypeptide chain of the epstein-barr virus major envelope glycoprotein and candidate vaccine molecule gp340.the epstein-barr virus (ebv) major envelope glycoprotein gp340 is the subject of current efforts to develop an ebv subunit vaccine. the importance of gp340-specific humoral immunity has been highlighted by studies of natural infection in humans and gp340 immunization of experimental animals. the former studies have demonstrated the presence of gp340-specific serum antibodies which mediate ebv neutralization, complement fixation, and antibody-dependent cellular cytotoxicity. the latter studies ha ...19921370550
transcription of the epstein-barr virus gene ebna-1 from different promoters in nasopharyngeal carcinoma and b-lymphoblastoid cells.transcriptional expression of the epstein-barr virus (ebv) genome has been shown to differ markedly between nasopharyngeal carcinoma (npc) cells and latent b-cell lines, with a more limited pattern of gene expression seen in npc. ebna-1 is the only nuclear antigen so far detected in both npc and burkitt's lymphoma cells. we found previously that in a human npc tumor passaged in nude mice, designated c15, the ebna-1 mrna contained a novel splice site in the bamhi q region of ebv which had not pre ...19921370554
levels of direct-acting mutagens, total n-nitroso compounds in nitrosated fermented fish products, consumed in a high-risk area for gastric cancer in southern china.a high gastric cancer mortality in fujian province (peoples republic of china) has been associated with the consumption of certain salted fermented fish products such as fish sauce (fs). we have investigated the levels and nature of n-nitroso compounds (noc) and genotoxins present, before and after nitrosation, in 49 fs samples collected from villages in this high-risk area, pooled into six samples. the concentrations of total noc before nitrosation ranged from 0.2 to 16 mumoles/l, and after nit ...19921370720
failure of epstein-barr virus-specific cytotoxic t lymphocytes to lyse b cells transformed with the b95-8 strain is mapped to an epitope that associates with the hla-b8 antigen.there are two types, a and b, of epstein-barr virus (ebv) and b95-8 represents the common type a laboratory strain. herein, we show in a family study that paternal ebv-specific cytotoxic t lymphocytes (ctl) generated in short-term cultures following stimulation with the autologous b95-8-transformed lymphoblastoid cell line (lcl) or b cells freshly infected with the b95-8 isolate did not lyse haploidentical b95-8 lcl expressing the hla-a1, -b8, -dr3 paternal haplotype. in contrast, the haploident ...19921370774
host-cell-phenotype-dependent control of the bcr2/bwr1 promoter complex regulates the expression of epstein-barr virus nuclear antigens 2-6.epstein-barr virus nuclear antigens (ebnas) are expressed in a cell-phenotype-dependent manner. ebna 1 is regularly expressed in all epstein-barr virus-carrying cells, whereas ebnas 2-6 are only expressed in epstein-barr virus-carrying cells with a lymphoblastoid phenotype including group iii burkitt lymphoma (bl) lines positive for b-cell activation markers. transcripts are initiated at the bcr2 or exceptionally at one bwr1 promoter in lymphoblastoid cell lines and group iii bl lines. in group ...19921371012
differential effects of human recombinant interferons on the expression of two early gene products of epstein-barr virus.two human burkitt's lymphoma (bl) cell lines, raji and daudi, have been previously characterized as resistant and sensitive, respectively, to the anti-epstein-barr virus (ebv) effects of human leukocyte interferon. these cells are equally susceptible to p3hr-1 ebv superinfection as determined by ebv early antigen (ea) expression. the cell lines were pretreated with human recombinant interferons alpha 2, beta, or gamma and subsequently superinfected with p3hr-1 ebv. their expression of two distin ...19921371044
a new method for detachment of dynabeads from positively selected b lymphocytes.this paper describes a method for the detachment of immunomagnetic beads from positively selected human b lymphocytes. after rosetting of b cells using anti-cd19 coated magnetic beads (dynabeads m-450 pan b, dynal), the dynabeads were rapidly detached (efficiency 80%) from the cells using goat anti-mouse-fab antiserum (detachabead, dynal) at ambient temperature. isolated b cells did not show significant differences in the expression of a number of b cell antigens when compared to b cells stained ...19921371532
colonization factor antigen cfa/iv (pcf8775) of human enterotoxigenic escherichia coli: nucleotide sequence of the cs5 determinant.human enterotoxigenic escherichia coli isolates expressing the colonization factor antigen cfa/iv (previously designated pcf8775) produce plasmid-encoded cs5 fimbriae. the nucleotide sequence of the region encoding the major cs5 fimbrial subunit was determined. the subunit is synthesized as a precursor of 203 amino acids (20.85 kda) with a mature protein of 181 amino acids corresponding to a size of 18.6 kda. the cs5 subunit shows homology to the corresponding component of porcine enterotoxigeni ...19921371766
interleukin 10 is a potent growth and differentiation factor for activated human b lymphocytes.interleukin 10 (il-10), originally identified as a th2 helper t-cell product able to inhibit cytokine production by th1 cells, is highly homologous to bcrf1 (viral il-10), an open reading frame in the epstein-barr virus genome. here, we show that human and viral il-10 stimulate dna replication of b lymphocytes activated either via their antigen receptor or via their cd40 antigen. il-4 and il-10 display additive effects and induce a strong increase in the number of viable cells. moreover, il-10 i ...19921371884
replication of adeno-associated virus type 2 in human lymphocytic cells and interaction with hiv-1.adeno-associated virus (aav) is a nonpathogenic parvovirus which normally requires helper adenovirus or herpes-virus for replication. we examined the growth of aav type 2 in human lymphocytes and its possible interaction with hiv-1. three b cell lines (ck-b, hs-2, and uc729) and four t cell lines (molt-4, jurkat, hut78, and hut78+hiv, which is persistently infected with hiv-1) were infected with aav either in the presence or in the absence of adenovirus. aav dna was found in cells of all the lin ...19921372138
t-cell lymphoma and the virus-associated hemophagocytic syndrome.a 20-month-old girl had a disorder that by both clinical and histologic criteria resembled the virus-associated hemophagocytic syndrome in the setting of epstein-barr virus infection. subsequent investigation revealed histologic evidence of disseminated t-cell lymphoma. dna hybridization studies displayed a monoclonal t-cell receptor beta chain rearrangement, in the absence of clonal immunoglobulin gene rearrangement, and a single band in the analysis for the fused termini of the epstein-barr vi ...19921372145
diagnosis of hairy leukoplakia by exfoliative cytologic methods.the cytologic characteristics of hairy leukoplakia (hl) are described based on the findings observed in four lesions. all cases were confirmed by histologic study, and epstein-barr virus dna was detected by means of in situ hybridization of tissue sections. all smears from the lesions exhibited a distinctive appearance and three types of epithelial changes were observed: (1) intranuclear inclusions of cowdry type a, (2) intranuclear inclusions with a ground-glass appearance, and (3) clumping and ...19921372148
increase of peripheral b lymphocytes committed to the production of monoreactive and high affinity antibodies to htlv-1 in patients with ham/tsp.we quantitated the frequency of b lymphocytes capable of producing antibodies to htlv-1 in the peripheral blood from patients with ham/tsp, non-ham/tsp htlv-1 carriers and seronegative healthy subjects. epstein-barr virus (ebv) was used as a polyclonal activator of b lymphocytes in a limiting dilution condition. we found that b lymphocytes committed to the production of monoreactive-igg and -iga antibodies to recombinant htlv-1 (gag + env) hybrid protein were significantly increased in a number ...19921372329
development and characterization of human anti-hbs antibodies.peripheral blood mononuclear cells (pbmc) from 13 healthy hepatitis b vaccines were transformed with the epstein-barr virus (ebv) and lymphoblastoid cell lines (lcl) producing antibodies to hepatitis b surface antigen (anti-hbs antibodies). seven lcl and two clones secreting human anti-hbs monoclonal antibody were generated and their antibodies purified. one clone was fused with a mouse myeloma and the antibody from a cloned anti-hbs secreting heterohybridoma purified. one of the 10 purified hum ...19921372611
elevated expression of icam1 (cd54) and minimal expression of lfa3 (cd58) in epstein-barr-virus-positive nasopharyngeal carcinoma cells.undifferentiated nasopharyngeal carcinoma (npc) is a remarkable entity among human tumors because of its constant association with the epstein-barr virus (ebv). malignant epithelial cells harbor the ebv genome and often express at least 2 species of latent ebv protein (ebna1 and lmp1). despite the massive presence of tumor-infiltrating lymphocytes, npc cells obviously escape immune surveillance directed to ebv antigens. previous investigations carried out on ebv-positive burkitt lymphoma (bl) ce ...19921372880
detection and localization of epstein-barr viral genomes in angioimmunoblastic lymphadenopathy and angioimmunoblastic lymphadenopathy-like lymphoma.we studied 23 cases of angioimmunoblastic lymphadenopathy (aild) and aild-like lymphoma for evidence of epstein-barr virus (ebv) using the polymerase chain reaction (pcr) and in situ hybridization studies. ebv nucleic acid sequences were found by either pcr or in situ hybridization in 96% of the cases. there was a wide range in the number of ebv-positive cells among the different cases as detected by in situ hybridization. the ebv-positive cells most often possessed nuclei of intermediate to lar ...19921373088
cytotoxic t cell lines recognize autologous and allogeneic melanomas with shared or cross-reactive hla-a.cytotoxic t lymphocytes (ctl), cd3+, alpha/beta t-cell-receptor-positive, are important effector cells with specific immunity in melanoma patients. the establishment and expansion in vitro of ctl of a specific phenotype to tumor cells strongly depends on the method of activation and sensitization with tumor cells. we generated cd3+ ctl lines to melanoma by co-culturing peripheral blood lymphocytes with autologous irradiated melanoma cells and repetitive stimulation with high-dose interleukin-4 i ...19921373343
human t lymphotropic virus types i- and ii-specific antibody-dependent cellular cytotoxicity: strain specificity and epitope mapping.antibody-dependent cellular cytotoxicity (adcc) against human t lymphotropic virus (htlv) types i and ii was investigated using sera obtained from infected individuals or from rabbits immunized with htlv-i or -ii envelope peptides. target cells included an htlv-i-transformed cell line (c91/pl), an htlv-ii-transformed cell line (729ph6neo), and epstein barr virus (ebv)-transformed b lymphocytes expressing htlv-i or -ii env or gag gene products after infection with vaccinia/htlv recombinants. adcc ...19921373751
infectious respiratory syncytial virus (rsv) effectively inhibits the proliferative t cell response to inactivated rsv in vitro.the effect of respiratory syncytial virus (rsv) on the cellular immune response of human mononuclear cells in vitro was examined. inhibition by rsv of the lymphocyte response to phytohemagglutinin in vitro was confirmed using cells from human umbilical cord blood. in addition, rsv significantly inhibited both the proliferative and t cell colony responses of human mononuclear cells to epstein-barr virus. an rsv-specific cellular immune response was induced in vitro by stimulation of mononuclear c ...19921373752
characterization of the 5' untranslated region of the human c-fgr gene and identification of the major myelomonocytic c-fgr this study, we have characterized the 5' region of the human c-fgr proto-oncogene and identified the major myelomonocytic c-fgr promoter. seven distinct 5' untranslated exons were identified and localized to a region extending 13 kb upstream from the first coding exon. two major promoters were identified, one utilized exclusively in epstein-barr virus (ebv)-infected b-lymphocyte cell lines, and the other functional only in myelomonocytic cells. differential promoter utilization and alternativ ...19921373875
identification and analysis of a novel human surface cd5- b lymphocyte subset producing natural antibodies.the production of "natural" autoantibodies or antibodies, i.e., ig that bind a variety of self- and/or exogenous ag and arise independently of known immunization, is though to be a feature of cd5+ b lymphocytes. to determine whether other lymphocyte subsets exist that might be committed to the production of natural antibodies, human peripheral blood b cells were sorted on the basis of surface cd5 expression and differential expression of surface cd45ra (cd5+cd45raintermediate(int), cd5-cd45ralow ...19921374094
growth- and differentiation-associated expression of bcl-2 in b-chronic lymphocytic leukemia cells.the bcl-2 gene is translocated into the ig loci in about 80% of human follicular lymphomas and in 10% of b-type chronic lymphocytic leukemias (b-cll), resulting in a high level of expression. we have compared the expression of bcl-2 transcripts and protein in b-cll cells in their normal equivalent cd5+ b cells and in normal b-cell populations representative of different in vivo and in vitro stages of activation and proliferation. we report here that bcl-2 was expressed in 11 of 11 cases of cd5+ ...19921375120
self-peptide released from class ii hla-dr1 exhibits a hydrophobic two-residue contact motif.peptide fragments of foreign and self-proteins are of great immunologic importance as their binding to major histocompatibility complex (mhc) class i or ii molecules makes an interaction with a corresponding t cell receptor possible. recently, allele-specific peptide sequence motifs proved to be responsible for mhc binding, no matter whether self- or non-self-antigens were involved. up to now, all investigated human class ii-associated peptides were derived from foreign antigenic proteins. there ...19921375272
irreversible association of peptides with class ii mhc molecules in living cells.functional, morphological and biochemical evidence indicates that class ii major histocompatibility complex (mhc) molecules associate with processed peptides during biosynthesis. peptide/mhc complexes in living cells have been reported to be less stable than similar complexes generated in vitro, which has led to the suggestion that there may be a peptide exchange mechanism operating in vivo. although this could increase the capacity for binding incoming antigens, it would reduce the efficacy of ...19921375347
definition of immunogenic determinants of the human papillomavirus type 16 nucleoprotein e7.specific t lymphocyte lines and t cell clones were established from peripheral blood mononuclear cells of asymptomatic seropositive individuals employing synthetic peptides which correspond to the sequence of the human papillomavirus (hpv) type 16 transforming protein e7. specificity analysis of t cells as determined by means of [3h] thymidine incorporation after stimulation with individual peptides revealed three immunogenic determinants of e7 that are recognised in association with at least tw ...19921375481
expression of the apo-1 antigen in burkitt lymphoma cell lines correlates with a shift towards a lymphoblastoid phenotype.apo-1 is a cell surface molecule that induces apoptosis when ligated with the monoclonal antibody anti-apo-1. expression of apo-1 and response to anti-apo-1 was investigated in a number of epstein-barr virus (ebv)-positive and -negative burkitt lymphoma (bl) cell lines, in ebv-immortalized lymphoblastoid cell lines, and in cells from fresh bl biopsies. apo-1 was not expressed in ebv-negative cell lines and in ebv-positive bl cell lines with a phenotype corresponding to bl tumor biopsy cells (cd1 ...19921375849
surface and molecular expression of complement-receptor type 2 of neoplastic cd5+ b cells in chronic lymphocytic leukemia. 19921376070
specificity and idiotope expression of igm produced by cd5+ and cd5- cord blood b-cell clones.epstein-barr virus (ebv)-immortalized monoclonal b-cell lines were established from cd5+ and cd5- cord-blood b cells. igm from many of both cd5+ and cd5- clones reacted with igg-fc, ssdna, and a variety of other autoantigens. more cd5+ b cells that used light chains of the kappa isotype reacted with igg-fc and ssdna than kappa-bearing cd5- b cells. because many of the clones reacted with igg-fc, they were analyzed for the expression of cross-reactive idiotypes (cri) associated with rheumatoid fa ...19921376073
spontaneous igm autoantibody production in vitro by b lymphocytes of normal human neonates.human neonate b lymphocytes display unique phenotypic and functional characteristics: in addition to cd1c antigens, cd5+ and cd5- subsets both express activation markers such as cd23 and bac-1. they proliferate strongly in the presence of various lymphokines (ril-2, ril-4, low molecular weight bcgf), but differentiate poorly in the presence of the same lymphokines, pokeweed mitogen and epstein-barr virus. it has also been reported that human neonate b lymphocytes produce polyreactive autoantibod ...19921376488
distribution of epitopes within the amino acid sequence of the epstein-barr virus major envelope glycoprotein, gp340, recognized by hyperimmune rabbit sera.epstein-barr virus (ebv) is a major human pathogen for which the development of an effective vaccine remains an important goal. rabbits were immunized with one of a set of 10 fusion proteins representing protein fragments from the ebv receptor-binding ligand and candidate subunit vaccine gp340. sera from recipients of fragments from the amino-terminal half of the polypeptide chain bound gp340 in western blot assays and elisa but were not virus-neutralizing. the fine epitope specificity of these ...19921376768
localization of epstein-barr virus cytotoxic t cell epitopes using recombinant vaccinia: implications for vaccine development.there is considerable interest in designing an effective vaccine to the ubiquitous epstein-barr virus (ebv). an important role for ebv-specific cytotoxic t lymphocytes (ctls) in eliminating virus-infected cells is well established. limited studies using a small number of immune donors have defined target epitopes within the latent antigens of ebv. the present study provides an extensive analysis of the distribution of class i-restricted ctl epitopes within ebv-encoded proteins. using recombinant ...19921377222
reactivity and self-association in vivo of a human monoclonal igg rheumatoid order to study the pathogenic potential of igg rheumatoid factor (igg-rf) we generated a human monoclonal igg4-rf-producing cell line, or-1, by epstein-barr virus transformation of b cells derived from a healthy donor. characterization of or-1 rf specificity demonstrated that this rf binds only to igg and not to dsdna or seven different proteins tested. although both or-1 rf and c1q bind to the fc part of igg, no influence could be observed of or-1 rf on the complement-fixing potential of hea ...19921377400
immune surveillance against epstein-barr virus.following primary infection, ebv retains a life-long latent association with b lymphocytes and a permissive association with stratified epithelium in the oropharynx. this review presents a model for the host-virus relationships in healthy virus carriers, a relationship which, if perturbed, may result in ebv-associated disease. cytotoxic t cells that recognise virally-determined epitopes on infected cells are the major effector arm and control the persistant infection. a strategy for developing a ...19921377517
conserved patterns of somatic mutation and secondary vh gene rearrangement create aberrant ig-encoding genes in epstein-barr virus-transformed and normal human b lymphocytes.expression of n-terminal-truncated ig heavy chains without normal light chain expression has been shown to occur in human b cell tumor lines, and to be due to diverse types of structural alteration within the expressed ig heavy and light chain genes. due to the tumor cell origin of these lines, generation of aberrant ig-encoding genes may only occur after malignant transformation, reflecting the release of the tumor b cell from the need to express functional ig for continued clonal proliferation ...19921377945
restoration of the lfa-3 adhesion pathway in burkitt's lymphoma cells using an lfa-3 recombinant vaccinia virus: consequences for t cell recognition.conjugate formation between cytotoxic t lymphocytes (ctl) and target b cells, as observed in vitro, is mediated by interactions between adhesion molecules on the two cell surfaces rather than involving immune recognition through the t cell receptor. it is still not clear to what extent such adhesive contacts facilitate the process of immune recognition and target cell lysis. however, work on the epstein-barr virus (ebv)-associated malignancy burkitt's lymphoma (bl) has suggested that down-regula ...19921378014
expression of bcl-2 in burkitt's lymphoma cell lines: induction by latent epstein-barr virus genes.the bcl-2 oncogene blocks programmed cell death (apoptosis). epstein-barr virus (ebv) can immortalize b lymphocytes into continuously growing lymphoblastoid cell lines (lcl) by the coordinate expression of at least 9 latent genes (ebv nuclear antigen [ebna] 1-6, latent membrane protein [lmp], and terminal proteins [tp] 1 and 2). we analyzed transcription and expression of bcl-2 and latent ebv genes in burkitt's lymphoma (bl) cell lines with a germinal center phenotype (group i) as well as activa ...19921378321
molecular basis for the association between hla dr4 and rheumatoid arthritis. from the shared epitope hypothesis to a peptidic model of rheumatoid arthritis.susceptibility to rheumatoid arthritis (ra) maps to residues qkraa/qrraa in the third hypervariable region of the hla dr beta 1 chain. peptides from the same area of mhc class ii molecules are able to modulate the t-cell repertoire by deleting self-reactive t-cells. the epstein barr virus glycoprotein gp110 and the dna j heat-shock protein from e. coli mimic the third hypervariable region of hla-dw4dr beta 1. thus, the same area of hla dr beta 1 carries susceptibility to ra, modulates the t-cell ...19921378777
identification of new epitopes recognized by human monoclonal antibodies with neutralizing and antibody-dependent cellular cytotoxicity activities specific for human t cell leukemia virus type 1.we have generated a number of ebv-transformed b cell lines producing human mab against human t cell leukemia virus type 1 (htlv-1) from the peripheral blood b lymphocytes obtained from patients with htlv-1-associated myelopathy/tropical spastic paraparesis. various synthetic peptides corresponding to antigenic regions of htlv-1 gag and env proteins were used for the screening of antibodies in elisa. in our study, four igg mab to the gag p19 amino acids 100 to 130, and 5 igg mab to the env p46 am ...19921378869
antibodies against epstein-barr nuclear antigen (ebna) in multiple sclerosis csf, and two pentapeptide sequence identities between ebna and myelin basic protein.the epstein-barr virus (ebv) causes infectious mononucleosis and is linked to several disparate malignancies. prior studies on patients with multiple sclerosis (ms) showed that 100% are ebv-seropositive and that their blood contains higher antibody titers than those of controls to both transformation and lytic cycle antigens. we performed three different assays for antibodies in csf to three major ebv antigens from patients with ms and controls. among 93 patients with ms, 79 (85%) had csf that r ...19921381067
dehydroepiandrosterone (dhea) and synthetic dhea analogs are modest inhibitors of hiv-1 iiib replication.down-regulation of epstein-barr virus (ebv) induced transformation of human lymphocytes in vitro by dehydroepiandrosterone (dhea), a naturally occurring human steroid secreted by the adrenal gland has been demonstrated. this article reports on the effects of dhea and its novel synthetic analogs 16 alpha-fluoro-5-androsten-17-one (8354) and 3 beta-hydroxy-16 alpha-fluoro-5 alpha-androstan-17-one (oh8356) on human immunodeficiency virus (hiv-1) replication. treatment with dhea, 8354, or oh8356 res ...19921381206
discrimination of human cd4 t cell clones based on their reactivity with antigen-presenting t this report, we describe the discrimination of human t cell clones based on their reactivity with activated t cells as antigen-presenting cells (apc). cd4+ t cell clones specific for peptide p30 of tetanus toxin (amino acids 947-967) and restricted to the dp4 molecule were established and tested for proliferation to peptide presented either by peripheral blood mononuclear cells (pbmc), epstein-barr virus (ebv)-transformed b cells or major histocompatibility complex (mhc) class ii-expressing t ...19921381313
epstein-barr virus latent membrane protein expression by hodgkin and reed-sternberg-like cells in acute infectious the light of reports of latent membrane protein (lmp) expression by hodgkin and reed-sternberg (hrs) cells, paraffin sections of tonsil (two cases), lymph nodes (eight cases; three cervical, one axillary, and four inguinal) and spleen (four cases) from 14 patients with acute infectious mononucleosis (im) have been examined for the presence of hrs-like cells and immunostained with an antibody to lmp. sections of the tonsils and one lymph node were also stained with a panel of antibodies which ...19921381432
in situ hybridization: a valuable tool in diagnostic situ hybridization or hybridohistochemistry has evolved in recent years in a new histologic modality. in situ hybridization (ish) can be used for the detection of dna (dish) or rna (rish). the potential diagnostic value within a pathologic setting are well recognized. in this review paper, we summarize the use of dish in a pathologic setting for the detection of chromosomal aberrations and localization of dna-viruses like cytomegalovirus and epstein barr virus. rish which is still in a more e ...19921381593
human monoclonal islet cell antibodies from a patient with insulin-dependent diabetes mellitus reveal glutamate decarboxylase as the target antigen.the autoimmune phenomena associated with destruction of the beta cell in pancreatic islets and development of type 1 (insulin-dependent) diabetes mellitus (iddm) include circulating islet cell antibodies. we have immortalized peripheral blood lymphocytes from prediabetic individuals and patients with newly diagnosed iddm by epstein-barr virus transformation. igg-positive cells were selected by anti-human igg-coupled magnetic beads and expanded in cell culture. supernatants were screened for cyto ...19921382289
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