epstein-barr virus infections in the x-linked recessive lymphoproliferative syndrome.prospective studies demonstrated variable phenotypic expression of the x-linked recessive lymphoproliferative syndrome (x.l.r.l.s.) in three brothers: (1) hypogammaglobulinaemia and subclinical epstein-barr-virus (e.b.v.) infection with antibody response to e.b.v.; (2) e.b.v. infection with defective immune response to e.b.v., fatal infectious mononucleosis (i.m.), and immunoblastic lymphoma; and (3) histiocytic lymphoma. hypogammaglobulinaemia and measles pneumonitis had preceded infection with ...197885816
false-positive serology in infectious monoucleosis. 197985957
x-linked immunocompetence and epstein-barr virus oncogenesis. 197986133
cell-mediated immunity to epstein-barr virus in nasopharyngeal carcinoma. 197986139
amplified direct immunofluorescence (amdi) for detection of epstein-barr virus nuclear antigen.low antigen concentrations could be identified in human cells by sequential application of (a) fitc-labeled human antibodies at high dilution, (b) rabbit antiserum to the hapten fluorescein isothiocyanate (fitc), and (c) fitc-anti-rabbit globulin. the high specificity of direct immunofluorescence (a) was not affected by the amplifying steps (b) and (c). using this amdi technique epstein-barr (eb) virus nuclear antigen (ebna) could be specifically stained with human sera up to a dilution of 1 : 4 ...197986587
adenovirus infection and heterophile antibody production. 197986693
a novel surface antigen on lymphoid cells transformed by epstein-barr virus.a surface antigen (sa) was detected on ebv-carrying lymphoid cell lines by an indirect membrane immunofluorescence test with serum from rabbits immunized with raji cells; the antiserum had been extensively absorbed with normal human blood and tonsil cells. the sa was not detected on normal human umbilical-cord and adult peripheral blood lymphocytes or ebv-negative cell lines. the incidences of the sa and ebv-determined membrane antigen (ma) on certain ebv-carrying cell lines were not compatible. ...197887368
limited immunodeficiency. 197887559
some progress with nasopharyngeal carcinoma. 197987621
evidence for antigenic distinctness of the epstein--barr virus-determined nuclear antigen and the herpesvirus papio-determined nuclear antigen.previous serological evidence indicated that epstein--barr virus-determined nuclear antigen (ebna) has cross reactive components with the herpesvirus papio-determined nuclear antigen (hupna) and, in addition, it has distinct components of its own, not present in hupna. in the present study, this hypothesis was studied by absorption experiments. absorption of anti-ebna positive human sera with ebna abolished both anti-ebna and anti-hupna reactivity. absorption with hupna removed all anti-hupna re ...197988257
abrogation of cell-mediated immunity by a serum blocking factor isolated from patients with infectious mononucleosis.lymphocytes from 80% of patients with infectious mononucleosis in this study failed to produce macrophage migration-inhibition factor in response to partially purified early antigen of epstein-barr virus or to tetanus toxoid, whereas lymphocytes from normal subjects did produce this lymphokine. subsequent analysis of serum from the patients with infectious mononucleosis revealed a serum factor that completely abrogated antigen-specific inhibition of migration by human leukocytes as well as lymph ...197988489
the antiherpesvirus action of phosphonoacetate. 197988744
search for epstein-barr and type c oncornaviruses in systemic lupus erythematosus.lymphoblastoid cell lines were derived from patients with active systemic lupus erythematosus by allowing spontaneous transformation of peripheral b lymphocytes (b cells) harboring endogenous epstein-barr virus or by superinfecting peripheral lymphocytes with exogeneous epstein-barr virus. results of extensive studies aimed at identifying type c oncornaviruses in these lymphoblastoid cells were entirely negative by electron microscopy, dna-dna hybridization, reverse transcriptase assays, and coc ...197988943
epstein-barr virus-induced membrane antigens: immunochemical characterization of triton x-100 solubilized viral membrane antigens from ebv-superinfected raji an attempt to qualitatively identify the membrane antigen (ma) complex induced by epstein-barr virus (ebv) infection of lymphoblastoid cells, superinfected raji cells were surface labelled with 125i by the lactoperoxidase method and solubilized with triton x-100, then the 125i-labelled membrane proteins were precipitated by sera containing high antibody titers to ma. analysis of these immune precipitates on sodium dodecyl sulfate polyacrylamide gel eletrophoresis identified four major ebv-spe ...197989099
differential inducibility of epstein-barr virus in cloned, non-producer raji cells.cells of the human lymphoblastoid non-producer line raji were cloned in soft agar. individual colonies were isolated and analyzed for their inducibility of the epstein-barr virus-associated early antigen (ea). the induction of ea by the tumor promoter tpa varied among the different cell clones. clones with very high and very low inducibility of the resident epstein-barr virus genome were further analyzed. constant differences in the inducibility of ea were observed after activation by tumor prom ...197989100
perinatal infection by epstein-barr virus. 197989313
thymidine sensitivity of cultured leukaemic lymphocytes.cultured leukaemic lymphocytes from patients with t, null, or pre-b acute lymphoblastic leukaemia are shown to be highly sensitive to growth inhibition by thymidine. thymidine sensitivity was correlated with reduced activity of the catabolic enzyme thymidine phosphorylase and sustained elevation of the deoxythymidine triphosphate pool after exposure to thymidine. it is suggested that thymidine may have a role in the management of certain acute lymphoblastic leukaemias of lymphomas.197989451
reciprocal 8;14 translocation in ebv-negative b-cell acute lymphocytic leukemia with burkitt-type cells.a cytogenetic study, including prophase-prometaphase chromosome analysis, of a patient with ebv-genome-negative acute lymphocytic leukemia of b-cell type with burkitt-type cells is presented. all bone-marrow mitoses examined had a 14q+ marker chromosome which was identified as a reciprocal 8;14 translocation of exactly the same type as in burkitt's lymphoma.197990020
increased tendency to spontaneous in-vitro lymphocyte transformation in clinically active multiple sclerosis.lymphocytes from 8 of 10 patients with clinically active multiple sclerosis (ms) but from only 3 of 18 patients with quiescent ms and 4 of 20 healthy donors transformed spontaneously on in-vitro culture. the transformed cells from all donors had the characteristics of b lymphocytes (surface and intracytoplasmic immunoglobulin and complement receptors) and carried antigens of epstein-barr virus. it is suggested that these results are further evidence that immunoregulation in active ms is abnormal ...197990801
site of epstein-barr virus replication in the oropharynx.cells and cell-free material in saliva, parotid secretions, and throat washings from patients with acute infectious mononucleosis and patients undergoing tonsillectomy were assayed for the presence of infectious epstein-barr (ebv) virus. the agent was invariably present in cell-free form in saliva; neither infectious virus nor viral antigens were found in the cells. tonsillar lymphocytes from eight patients were also free of ebv. in 10 of 40 patients virus was recovered in secretions from paroti ...197991888
establishment of a lymphoblastoid cell line and isolation of an epstein-barr-related virus of gorilla origin.a b-lymphoid cell line was established from a normal gorilla. the cells contained epstein-barr virus-related antigens, and herpesvirus particles were demonstrated by electron microscopy. dna-dna reassociation kinétics revealed 30 to 40% hybridization to epstein-barr virus with 50 genomes per cell. examination of the viral nuclear antigen with gorilla sera showed this to be a unique isolate termed herpesvirus gorilla. h. gorilla transformed gibbon b-lymphocytes in vitro.197992573
correlation between epstein-barr virus membrane antigen and three large cell surface glycoproteins.a correlation between epstein-barr virus membrane antigen (ma) and three surface glycoproteins has been established on the basis of radio-immunoprecipitation and immunoabsorption experiments. for radio-immunoprecipitation, epstein-barr virus-infected cells were radiolabeled either with neuraminidase-galactose oxidase tritiated borohydride, a procedure highly specific for surface glycoproteins, or with a general tritiated amino acid mixture. intact cells were incubated with ma(-) or ma(+) human s ...197992577
education of mouse lymphocytes against human lymphoblastoid cell lines: specific recognition of determinants independent of serologically defined hla allotypes and epstein-barr virus-coded antigens. 197993053
[epstein-barr virus associated burkitt's lymphoma in a french child (author's transl)].pathologic diagnosis of malignant lymphoma, burkitt type, was made in a 12 year old child with an abdominal tumour. the association of this lymphoma with epstein-barr virus was done based on the detection of epstein-barr viral markers within the tumour cells, as well as on the patient's serology. to our knowledge, it is the first time that the association between burkitt's lymphoma and epstein-barr virus, which is frequent in africa, is reported in a caucasian french.197993275
epstein-barr virus-specific antibody-dependent cellular cytotoxicity in patients with burkitt's lymphoma.coded sera from 54 patients with african burkitt's lymphoma (bl) were titrated for antibodies against an epstein-barr virus (ebv)-induced membrane antigen in the antibody-dependent cellular cytotoxicity (adcc) assay. the titers were then correlated with the progression of lymphoma growth following chemotherapy. in 74% of the patients with high adcc titers (greater than 3,840), lymphomas showed partial or complete regression following therapy. in the medium-titered group (240-3,840), 36% of the l ...197993578
abortive expression of the epstein-barr virus (ebv) cycle in a variety of ebv dna-containing cell lines, as reflected by nucleic acid hybridization in situ.a variety of epstein-barr virus (ebv) dna-containing cell lines have been tested for the expression of the ebv-associated antigens ebna (nuclear antigen), ea (early antigen), and vca (viral capsid antigen), and for the presence of cells containing disproportionate amounts of ebv dna. the antigen tests utilized immunofluorescence and 125i-labelled antibodies combined with autoradiography. ebv-dna was detected by in situ hybridization with 3h-labelled ebv rna complementary to p3hr-1 ebv dna (p-ebv ...197993584
the simultaneous observations of immunofluorescence and cellular morphology in viral infections: a new procedure.a simple method for comparative observations of immunofluorescence and cellular morphology has been developed in the investigation of epstein-barr virus (ebv) infection and oncogenesis. when cells were stained for immunofluorescence of ebv-determined nuclear antigen, early antigens, or the viral capsid antigen, followed by treatment with hematoxylin, viral marker expression and cellular morphology could be simultaneously observed in individual cells by simply changing ultraviolet illumination to ...197895789
establishment of specific antibody producing human lines by antigen preselection and epstein-barr virus (ebv)-transformation.specific antibody producing human cell lines were established by preselecting antigen binding b-lymphocytes and subsequently transforming ("immortalizing") them with epstein-barr virus (ebv). nnp-binding b-cells were isolated from the blood of three donors with high anti-nnp titers. ebv-transformation led to polyclonal cell lines that had 15-18% nnp receptor positive cells. a similar fraction of the cells produced nnp-specific plaques in the cunningham-szenberg assay. unconcentrated culture medi ...197995800
herpesvirus papio: state and properties of intracellular viral dna in baboon lymphoblastoid cell lines.herpesvirus papio (hvp) is an indigenous b-lymphotropic virus of baboons (papio sp.) present in latent form in baboon lymphoblastoid cell lines. it shares cross-reacting viral capsid and early antigens with the epstein-barr virus (ebv), and hvp dna and ebv dna show partial sequence homology. ebv-specific complementary rna was employed here as a probe to investigate the physical state of the hvp dna component in baboon lymphoblastoid cells after fractionation of cellular dna by density gradient c ...1979113352
liver disease in patients undergoing hemodialysis and kidney transplantation.liver dysfunction was observed in 33% of patients treated by hemodialysis and kidney transplantation. fifty-eight percent of these cases of hepatitis occurred in patients with past or present hbs antigenemia, and 77% of hbsag-positive patients showed evidence of ld. however, during the course of a program conducted from 1969 to 1976 and involving 267 patients, the decrease in the prevalence of hbs antigenemia observed during the last two years did not lead to any reduction in ld incidence. in a ...1979115244
[classification of malignant lymphomas of the neck: combined morphological, immunocytological, serological and tissues culture studies (author's transl)].81 untreated malignant lymphomas of the neck were classified morphologically according to the german kiel classification and to the american classification of rappaport and berard and these tumors were typed immunologically as to their t- or b-cell nature. cells from 16 of these patients were subsequently grown in tissue culture for periods up to seven months. tissue culture cells were monitored as to spontaneous variations in the morphologic cell type and to the expression of t- or b-cell surfa ...1976136561
comparison of the stimulating capacity of human leukemia t-cell, null cell, and b-cell lines in mixed lymphoma mixed lymphocyte reactions between lymphocytes from normal human donors and mitomycin c-treated cells from human leukemia t-cell, null cell, and b-cell lines were investigated. an epstein-barr virus (ebv)-negative japanese burkitt's lymphoma line and two ebv-positive normal lymphoid cell lines were studied in parallei. normal lymphocytes were stimulated significantly by the cultured null cells and b-cells, but only slightly by the cultured t-cells. the stimulatory capacity of these two l ...1978147348
phosphofructokinase in human blood cells.the subunit composition of phosphofructokinase from normal and malignant blood cells has been investigated by means of immunologic, electrophoretic, and chromatographic methods. immunoprecipitation tests were performed with three specific antisera recognizing each of the basic subunits of human phosphofructokinase: muscle, m-type; liver, l-type; and fibroblast, f-type. mature polymorphonuclear cells contain mainly l-subunits, while lymphocytes and platelets contain hybrids formed of l and f subu ...1979156568
establishment and characterization of human b-lymphocytic lymphoma cell lines (balm-3, -4 and -5); intraclonal variation in the b-cell differentiation stage.this study describes the establishment of three non-burkitt b-lymphoma cell lines (balm-3, balm-4 and balm-5) originating from the pleural effusion of a patient with a poorly differentiated diffuse lymphocytic lymphoma. the cells of balm-3, -4 and -5 exhibited a number of properties which distinguish them from the usual b-cell type lymphoblastoid cell lines. thus, they lacked the epstein-barr virus genome and had abnormal chromosome constitutions including a 14q+ marker. the presence of the iden ...1979160894
induction of permanently proliferating human lymphoblastoid lines by n-methyl-n'-nitro-n-nitrosoguanidine.n-methyl-n'-nitro-n-nitrosoguanidine treatment of human peripheral blood mononuclear cells increases the frequency of lymphocyte transformation into permanently proliferating lines. the transformation is dependent on the dose of n-methyl-n-7-nitro-n-nitrosoguanidine and does not occur in the presence of autologous human serum. thirty-eight of the forty-two established lines resulting fron n-methyl-n'-nitro-n-nitrosoguanidine treatment produced detectable epstein-barr virus antigens.1975162872
primary epstein-barr-virus infections in acute neurologic diseases.infectious mononucleosis has been associated with guillain--barré syndrome, bell's palsy, meningoencephalitis and transverse myelitis. since it is not known that many children with infectious mononucleosis do not develop heterophil antibodies, we looked for evidence of current or recent epstein-barr virus infection in young patients with these neurologic diseases by using serodiagnostic procedures for detection and titration of antibodies to various antigens related to epstein-barr virus. seven ...1975163004
characterization of a serum inhibitor of mlc reactions. iii. specificity: hl-a-related antibodies. 1975163145
elevated immunofluorescence antibody titers to several herpesviruses in burkitt's lymphoma patients: are high titers unique?antibody titers for viral capsid antigens of all four human herpesviruses were measured by immunofluorescence in the sera of 16 burkitt's lymphoma (bl) patients, 16 age-, sex-, and locality-matched controls, and 136 family members from the west nile district of uganda. among family members, titers greater than 1:4 were found in 98% for herpes simplex virus (hsv), 86% for varicella-zoster virus (vzv), 100% for cytomegalovirus (cmv), and 94% for epstein-barr (ebv). titers in patients averaged appr ...1975163324
induction of epstein-barr virus-associated nuclear antigen during in vitro transformation of human lymphoid cells.human lymphoid cells isolated from the peripheral blood of adults, from cord blood, and from fetal liver, spleen, bone marrow, and thymus were cultivated with or without a cell-free preparation of epstein-barr virus (ebv) with demonstrated transforming activity. the cultures were examined for the ebv-associated nuclear antigen (ebna) and for transfromation into permanent lymphoblastoid cell lines (lcl). ebna, seen only in cultures that had received exogenous ebv, was detected between days 1 and ...1975163326
spontaneous lymphoma associated with herpesvirus saimiri in owl monkeys.two cases of lymphoma and one case of lymphoproliferative disease were found in a group of 7 owl monkeys imported into our colony as a single group. herpesvirus saimiri (hvs) was isolated from the tumor cells of 1 lymphoma by cocultivation and from kidney cell cultures from the monkey with lymphoproliferative disease. antibody to hvs was found in serum samples from 2 monkeys positive for hvs but not in the sera from the 4 clinically normal monkeys. antibody to epstein-barr virus-infected cells w ...1975163336
dna of epstein-barr virus. i. comparative studies of the dna of epstein-barr virus from hr-1 and b95-8 cells: size, structure, and relatedness.we have compared the properties of the dna of epstein-barr virus (ebv) purified from hr-1 (ebv hr-1 dna) and b95-8 (ebv b95-8 dna) continuous lymphoblast cultures. our data indicate that (i) the s suc of native ebv dna relative to t4d dna is 55s. using the modified burgi-hershey relationship (5), we estimate the molecular weight of native ebv dna is 101 (plus or minus the molecular weight of native fbv dna by measurement of the length of 3) times 106. estimation of the molecule relative to form ...1975163373
antibodies to epstein-barr-virus antigens before and after the development of burkitt's lymphoma in a patient treated for hodgkin's disease. 1975163432
transfusion-associated hepatitis not due to viral hepatitis type a or b.twenty-two patients who had an episode of transfusion-associated hepatitis not positive for hepatitis b antigen were examined for development of antibody to heaptitis a and b antigens, cytomegalovirus and epstein-barr virus. antibody response to the 27-nm virus-like hepatitis a antigen was measured by immune electron microscopy. in none of the 22 patients studied did serologic evidence of infection with hepatitis a virus develop during the study period. nine of the 22 patients had antibody respo ...1975163436
production of lymphoid tumours in hamsters by direct implantation of normal human leukocytes. 1975163447
infectious mononucleosis: the kissing disease. 1975163472
demonstration of a cellular inhibitor of epstein-barr and cytomegalovirus synthesis. 1975163544
clinical evaluation of patients with infectious mononucleosis and development of antibodies to the r component of the epstein-barr virus-induced early antigen complex.previous reports have emphasized that the transitory antibody responses to epstein-barr virus (ebv)-induced early antigens (ea) in the course of infectious mononucleosis are usually directed against the d (diffuse) component of the ea complex. in this report clinical and serologic data have been presented on 14 patients with infectious mononucleosis who responded either solely with antibodies to the r (restricted) component of the ea complex or revealed anti-r after the initial anti-d responses ...1975163582
human chronic myelogenous leukemia cell-line with positive philadelphia chromosome.a cell-line derived from a patient with chronic myelogenous leukemia (cml) is described. the new cell-line, which has over 175 serial passanges in a 3 1/2-yr period, has the following characteristics: (1) cml cells started to proliferate actively since they were first incubated in culture media. a threefold increase in the total number of cells was observed during the first seven passages; the cell population increased by a factor of 10 to 20 every 7 days from passage 8 through 85; from 20 to 40 ...1975163658
central failure of b-lymphocyte induction in pan-hypogammaglobulinemia. 1975163714
epstein-barr virus binding sites on lymphocyte subpopulations and the origin of lymphoblasts in cultured lymphoic cell lines and in the blood of patients with infectious mononucleosis. 1975163715
an established burkitt's lymphoma line with cell membrane igg. 1975163717
antibody response to epstein-barr virus in infectious mononucleosis.altogether 171 serum specimens from 58 patients with heterophil antibody-positive infectious monomucleosis were studied for antibody response to epstein-barr virus (ebv). the sera were tested for fluorescent immunoglobulin g (igg) and igm gel-precipitating (gp) and complement-fixing (cf) antibodies to ebv. all 58 patients had igg and igm antibodies to ebv. both igg and igm antibodies developed rapidly; the igm antibodies disappeared within 8 to 10 weeks, whereas the igg antibodies remained at an ...1975163790
seroepidemiologic study of epstein-barr virus infections in a rural community.the prevalence and titers of antibodies to capsid antigens of epstein-barr virus ad to the diffuse and restricted components of the epstein-barr virus-induced early antigen complex were determined in 109 families of a semirural community in louisiana. titers of antibody to the capsid antigens larger than or equal to 10 were found in 84 percent of children aged two to five years, and the prevalence increased with age to nearly 100 percent. there was a positive but variable correlation of the prev ...1975163869
letter: eb-virus-seropostivie person is susceptible to infectious mononucleosis. 1975163963
the densities of herpesviral dnas. 1975164053
oncogenic herpes viruses. 1975164249
preparation and spread of unfixed metaphase chromosomes for immunofluorescence staining of nuclear antigens. 1975164358
differences between soluble complement-fixing antigens derived from p3hr-1, raji, and hk-ly-28 cell lines.soluble complement-fixing (cf) antigens of the virus-producing and virus-nonproducing p3hr-1 and raji burkitt lymphoma cell lines and hk-ly-28 nasopharyngeal cancer cell line, identified by use of viral capsid antigen-positive human sera, could readily be distinguished by differences in their stability to chemical and physical conditions. the p3hr-1 soluble cf antigen lost titer when heated or exposed to acid perchlorate under conditions in which raji and hk-ly-28 soluble antigens were stable. d ...1975164559
viruses: causative agents of cancer.evidence accumulated over a period of 60 years has clearly established that a number of different viruses cause neoplasia (of a broad spectrum) amongst a variety of animals. although it is known that viruses do produce self-limiting proliferative human diseases, complete verification of a human viral carcinogen remains to be provided. this paper presents an overview of the conceptual and practical tools for the detection of tumor viruses, which have derived from experimental model systems. it in ...1975164594
studies on the presence of antibodies to eb virus and other herpesviruses in normal children and in infectious mononucleosis.children free from infectious disease have been examined by indirect immunofluorescence for the presence of antibodies to the intracellular capsid antigen of epstein-barr virus (ebv). in the first year of life 46%, between 2 and 6 years of age 66%, and between 7 and 14 years 91%, of the children proved positive. the corresponding percentages for the presence of antibodies to cytomegalovirus (cmv) and herpes simplex virus were about 50%, irrespective of the children's age. serum samples from 69 p ...1975164758
epidemiology of hodgkin's disease: review and etiologic leads. 1975165038
pilot experiments with eb virus in owl monkeys (aotus trivirgatus). iii. serological and biochemical findings in an animal with reticuloproliferative disease.nucleic acid hybridization with eb virus complementary rna has demonstrated unequivocally that eb virus dna is present in the cells of a continuous lymphoblastoid line established in culture from a pathological lymph node of an owl monkey with reticuloproliferative disease after inoculation with eb virus. there were 48 to 49 eb virus genome equivalents per cell. in addition, serological studies showed that the diseased owl monkey developed specific antibodies to eb virus capsid antigens. the ant ...1975165147
characteristics of cell lines derived from human leukocytes transformed by different strains of epstein-barr virus.variation of epstein-barr virus (ebv) in respect to its effect on the properties of transformed cells was probed. human umbilical cord leukocytes from six different individuals were transformed in vitro by either b95-8 (b) or qimr-wil (q) strains of ebv and subsequently 12 lymphoblastoid cell lines (six b-derived and six q-derived lines) were established. the b lines and q lines were different in the expression of ebv genome i.e. production of virus or viral antigens, and in other properties inc ...1975165154
classification and biological nature of established human hematopoietic cell lines.over 200 established human hematopoietic cell lines of normal and malignant origin have been investigated by morphological and functional parameters. employing morphology as the overriding parameter four types of lines were identified. (1) lymphoblastoid cell lines, derived from normal and neoplastic hematopoietic tissue, were characterized by the wide morphologic flexibility of individual lymphoblastoid cells, constant association with epstein-barr virus (ebv), polyclonal derivation, differenti ...1975165156
clinical aspects of infection with the epstein-barr virus. 1975165289
attempts to detect virus-specific dna sequences in human tumors. iii. epstein-barr viral dna in non-lymphoid nasopharyngeal carcinoma cells.fourteen tumors of the nasopharyngeal region were analyzed for the presence of epstein-barr virus-specific dna by dna-crna hybridization. these data were compared to the histology of the respective tumors and the seroreactivity of the tumor-bearing patient against ebv-related antigens. with one exception, all tumor pieces containing nasopharyngeal carcinoma cells hybridized significantly with ebv-crna. tumors of predominantly epithelial morphology annealed in the highest range. in situ-hybridiza ...1975165392
letter: what is the determinant for susceptibility to infectious mononucleosis? 1975165409
letter: burkitt's lymphoma in a patient treated for hodgkin's disease: one or two diseases? 1975165412
acquired agammaglobulinemia after a life-threatening illness with clinical and laboratory features of infectious mononucleosis in three related male children.three males in one family (two siblings and one maternal cousin) had an illness with cervical adenopathy, hepatosplenomegaly, and a fulminant febrile course. in the two survivors agammaglobulinemia developed. one of them became ill at the age of six months and had an epstein-barr-virus antibody titer of 1:10 during illness and convalescence. the white-cell count was 120,000 with 90 per cent lymphocytes, most being atypical and forming increased numbers of sheep erythrocyte rosettes. igm was elev ...1975165416
epstein-barr virus genomes with properties of circular dna molecules in carrier cells.a high-density fraction of high-molecular-weight dna was isolated from the human lymphoid cell line raji. this cell line contains 50 to 60 virus genome equivalents of epstein-barr virus dna per cell, and the high-density dna fraction was 10-fold enriched in such viral sequences. sedimentation analysis on neutral glycerol gradients, followed by hybridization experiments with viral complementary rna, showed that most of the intracellular viral dna sequences in this material did not cosediment with ...1975165511
cytotoxic effector cells specific for b cell lines transformed by epstein-barr virus are present in patients with infectious mononucleosis.peripheral lymphoid cells, from 12 cases of acute infectious mononucleosis (im), were tested in a micro chromium-51 release assay for cytotoxic activity against a variety of cell lines that did or did not carry the epstein-barr virus (ebv) genome. unfractionated lymphocytes from these patients were cytotoxic to both types of cell lines, as were lymphocytes from healthy individuals. if, however, lymphocytes bearing complement receptors were removed, the residual im lymphocyte fraction was specifi ...1975165518
virus antibody levels in serum specimens from patients with optic neuritis and from matched controls;.virus antibody levels in serum specimens taken in acute and convalescent phases from 77 patients with optic neuritits were tested by measles hamagglutination inhibition (hi), measles hemolysis inhibition (hli), rubella hi, parainfluenza-1 hi, epstein-barr immunofluorescence (if), and against 11 other viruses and mycoplasma pneumoniae with the complement fixation (cf) technique. the virus antibody levels were indicated to be usually very stable, and a fourfold change in virus antibody levels was ...1975165652
virus antibody levels in the cerebrospinal fluid from patients with optic neuritis.virus antibody levels were studied in the cerebrospinal fluid (csf) of 58 patients with optic neuritis and 58 control patients with no indication of multiple sclerosis (ms) or infectious disorders of the central nervous system (cns). the specimens were tested against three different structural components of measles virus with measles hemagglutination inhibition (hi), measles hemolysis inhibition (hli) and gel precipitation (gp) tests. measles antibodies occurred in 62 per cent of csf specimens f ...1975165653
infectious mononucleosis preceding acute myelomonocytic leukemia.the patient discussed in this report had acute myelomonocytic leukemia (amml) associated with infectious mononucleosis. infectious mononucleosis (im) in association with acute leukemia (al) has provided clinical investigators an active area of speculation for several years. nineteen cases are presented for review in table 1. the present case represents the second case of amml to be associated with im. it is the first case of a granulocytic leukemia reported in which active im is occurring simult ...1975165722
eb-virus-specific igm and igg antibodies in first-degree relatives of children with acute lymphoblastic leukaemia.eb-virus-specific igm and igg antibodies (to virus capsid and soluble complement fixing antigens) were estimated in sera from mothers and sibs of children with acute lymphoblastic leukaemia, from patients with infectious mononucleosis, and from control induviduals. igm antibodies were present in 12 of 16 mothers and 3 of 4 sibs of children with acute lymphoblastic leukaemia. they were also present in 14 of 16 patients with infectious mononucleosis, but in only 1 of 12 control individuals.1975165784
do viruses cause cancer in man? 1975165866
[superinfection of human lymphoblastoid cells with eb virus (author's transl)]. 1975165904
[preparation of suspension lymphoblastoid cell cultures, containing herpes-like viruses, from hematopoietic organs of hamadryas baboons with hematosarcoma]. 1975165924
biological differences between epstein-barr virus (ebv) strains with regard to lymphocyte transforming ability, superinfection and antigen induction. 1975165958
letter: infectious mononucleosis in children. 1975166350
[letter: burkitt's tumor. simultaneity in 2 sisters of ivory coast nationality]. 1975166354
viral and cellular dna synthesis in nuclei from human lymphocytes transformed by epstein-barr virus.a dna-synthesizing system in vitro, using nuclei prepared by treatment of human lymphocytes with the detergent brij 58, was developed. nuclei from cultured lymphocytes synthesized dna for as long as 5 hr, and required atp, deoxynucleoside triphosphates, magnesium, and a calcium chelator. in nuclei from a partially synchronized line of cultured lymphocytes carrying several hundred copies of the epstein-barr viral genome, synthesis in vitro was predominately viral in early s phase and cellular in ...1975166387
epstein-barr virus: comparison of different strains for their biological activities in vitro.several sources of epstein-barr virus (ebv) were tested for the ability to superinfect raji cells or to transform human cord blood leukocytes. ebv prepared from p3hr-1 cells showed only the ability to superinfect raji cells whereas ebv from qimr-wil cells from a cell line (cru-l1) derived from an infectious mononucleosis (im) patient or virus from throat washings from im patients transformed human cord blood leukocytes, but did not superinfect raji cells.1975166422
cellular cooperation in transformation by eb virus.human lymphoid cells transformed by epstein-barr (eb) virus are thought to be b-lymphocytes. transformation of human mononuclear leukocytes by epstein-barr virus was decreased or lost by removing adhering or nonadhering mononuclear cells from the mixtures of peripheral blood leukocytes but was not decreased by removing t-lymphocytes. recombining adherent and nonadherent mononuclear cells without t-lymphocytes restored transformability, but conditioned medium from cultures of adherent cells did n ...1975166423
eb virus-associated nuclear antigen production and cell proliferation in adult peripheral blood leukocytes inoculated with the qimr-wil strain of eb virus.a nuclear antigen, apparently the epstein-barr virus (ebv)-associated nuclear antigen (ebna), was detected by anticomplement immunofluorescence (acif) tests in adult peripheral blood leukocytes infected with the qimr-wil strain of ebv. ebna was not detectable at 24 h but appeared in about 11% of the cells by 3 days, and by 5 days up to 64% of the cells were positive. proliferation of ebna-positive cells at this stage was confirmed by autoradiography. there was a good correlation between the conc ...1975166936
the detection of the epstein-barr virus (ebv) nuclear antigen (ebna) by anticomplement immunofluorescence. immunoglobulin class of antibodies and role of complement.the epstein-barr virus (ebv)-associated nuclear antigen (ebna) was detected by anti-complement immunofluorescence (acif) in raji lymphoblastoid cell line, and the mechanism of the reaction explored, using ig fractions of anti-ebna sera and purified components of complement. following fractionation of serum from 13 donors, anti-ebna antibodies were always found to be associated with igg, but were also detectable in the igm fraction of four sera. sequential addition of functionally pure complement ...1975166939
immunofluorescence and anti-complement immunofluorescence absorption tests for quantitation of epstein-barr virus-associated antigens.immunofluorescence absorption methods are described which permit quantitative estimation and differentiation of epstein-barr virus (ebv)-associated antigens (virus capsid antigen, vca, early antigen, ea and ebv-determined nuclear antigen, ebna) in cell extracts. ebna was present in all cell lines (producer and non-producer) which carried the ebv-genome, while vca and ea were present in producer lines only. all the antigens were absent from a lymphoid cell line (molt-4) which lacked the ebv-genom ...1975166941
studies of epstein-barr virus (ebv)-associated nuclear antigen. i. assay in human lymphoblastoid cell lines by direct and indirect determination of 125i-igg binding.a quantitative assay for ebna in human lymphoblastiod cell lines has been developed. the assay employs ebna-positive and -negative 125-i-igg preparations as reagents and can be used in a direct or indirect manner. ebna specificity has been demonstrated in a number of ways. the antigenic relationship between ebna present in raji cells and in a number of other human lymphoblastiod cell lines of diverse origins and between the cell-associated antigen present in ft raji cells and that present in is ...1975166942
antibody patterns to herpesviruses in kaposi's sarcoma: serological association of european kaposi's sarcoma with cytomegalovirus.sera from patients with kaposi's sarcoma (ks) were examined for antibody titres to cytomegalovirus (cmv), epstein-barr virus (ebv) and herpes simplex virus (hsv) types 1 and 2 by four techniques: indirect haemagglutination (iha), complement fixation (cf), virus neutralization (nt) and indirect immunofluorescence (if). the patients were classified, according to the stage of disease, as progressive and regressive. control sera were obtained from healthy adults, matched for age, sex, race, socioeco ...1975166949
proceedings: observations on the nature of eb-virus infection of peripheral lymphoid cells in infectious mononucleosis. 1975167166
proceedings: cyotgenetic differences between burkitt lymphoma and other ebv-related human lymphoblastoid-cell lines. 1975167167
proceedings: selection of an ebna-positive variant from an ebna-negative human lymphoblastoid-cell line without detectable ebv dna. 1975167168
assay for epstein-barr virus based on stimulation of dna synthesis in mixed leukocytes from human umbilical cord blood.relationships between the rate of dna synthesis in cultured human umbilical cord leukocytes and the multiplicity of added epstein-barr virus (ebv) were studied. at low multiplicities of approximately 0.1 transforming units/cell (approximately 10 physical particles/cell), inoculated cultures demonstrated increased rates of dna synthesis, by comparison to uninoculated cultures, 3 days after inoculation. stimulation of dna synthesis was evident of progressively longer intervals after inoculations o ...1975167182
newer knowledge in comparative virology--its contribution to human health other comparative sciences, and despite its recent beginning comparative virology has already contributed useful applications and observations to human health research. teachings derived from the study of marek's disease found application in that of burkitt's lymphoma, and may lead to a possible vaccine against the human disease. equally useful information came from the study of canine distemper in the development of a chorio-allantoic membrane attenuated measles vaccine, and in our knowled ...1975167504
[pass-way of epstein-barr virus in the abortive infection of human lymphoid cells (author's transl)].biologically active, 3-h-thymidine-labeled epstein-barr virus has been purified by a method, specifically developed for these experiments, and used to infect the human lymphoid cell lines nc37 and raji. at a late stage of this abortive infection (after 40 hr), 3-h-dna has been recovered from the nuclei of the infected cells. this dna has the density and size of free viral dna. no evidence for integration was observed, in that less than 0,5 viral genome per cell had become covalently bound to cel ...1975167537
differential activation by cell fusion of epstein-barr viral and type-c viral genomes in non-productive human lymphoid cells. 1975167693
detection of viral cores having toroid structures in eight herpes viruses. 1975167709
reconstitution of t-cell disorders following thymus transplantation. 1975167882
relationship between epstein-barr virus-associated nuclear antigen and cell viability in human cell lines.the relationship between the cell viability and frequency of cell with epstein-barr virus (ebv)-associated nuclear antigen was investigated in cultures of 4 ebv-carrier lymphoblastoid cell lines, by means of anti-complement immunofluorescence. the percentages of viable cells and those of eb nuclear antigen-positive cells were close to each other in the entire course of cultivation of all cell lines. this leads to a conclusion that absence of eb nuclear antigen in a given cell population should b ...1975168112
epidemiology of herpesvirus and respiratory virus infections. part 1. serologic findings.the majority of the population group studied had complement-fixation antibodies toward the following viruses: influenza type a, respiratory syncytial, cytomegalovirus, and epstein-barr. the herpesvirus infections (cytomegalovirus and epstein-barr virus) seemed to be prevalent. only low incidences of antibodies were found toward adenovirus, influenza type b, influenza type c, and parainfluenza type ii. a total of 70 aucte virus infections (increases of antibody titer) were diagnosed in 49 patient ...1975168127
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