PMID(sorted ascending)
fireside conference 16. nasopharyngeal carcinoma. 19921327018
association of epstein-barr virus genome with mixed cellularity and cellular phase nodular sclerosis hodgkin's disease subtypes.association of epstein-barr virus (ebv) genome with hodgkin's disease (hd) histological subtypes was investigated using the polymerase chain reaction (pcr). a highly significant association of ebv genome with the mixed cellularity (mc) subtype (10 positive out of 15 cases; 66%) was observed, suggesting a possible etiopathogenetic role of ebv in the induction of this subset. by contrast, a markedly lower frequency of association with ebv genome was found in nodular sclerosis (ns) (12 positive out ...19921327078
epstein-barr virus transcription factors. 19921327084
[evaluation of igg-igm antibodies against the epstein-barr virus nuclear antigen (ebna-1) using elisa].evaluation of two commercial immunoenzymatic techniques (elisa) for detection of anti-ebna-1 igg and igm for diagnosis of epstein-barr virus mononucleosis.19921327168
heterogeneous epstein-barr virus infection patterns in peripheral t-cell lymphoma of angioimmunoblastic lymphadenopathy this study, 32 cases of t-cell lymphoma of angioimmunoblastic lymphadenopathy type (aild-tcl) were investigated for their association with epstein-barr virus (ebv). for this purpose, three different approaches were applied: polymerase chain reaction (pcr) for the presence of ebv-dna, in situ hybridization (ish) for ebv-encoded small nuclear rnas (eber), and immunohistology for ebv-encoded latent membrane protein (lmp). pcr and eber-ish produced almost identical results, showing that all but o ...19921327284
a rapid method for starting a culture for the establishment of epstein-barr virus-transformed human lymphoblastoid cell lines.we developed a simple and efficient procedure for the establishment of epstein-barr virus-transformed human lymphoblastoid cell lines. b-lymphocytes were obtained by centrifugation after hemolysis of red cells with a hemolysis buffer, instead of ficoll-parque gradient. we can start a primary culture within 15 min by using this method.19921327290
primary immunodeficiencies: genetic risk factors for has been estimated that up to 25% of patients with certain genetically determined immunodeficiencies will develop tumors, primarily b-cell lymphomas, during their lifetime. epstein-barr virus appears to be an important cofactor in the development of lymphoproliferative disorders in patients with primary immunodeficiencies, as well as acquired immunodeficiencies. additionally, host defects in immunoregulation and/or gene rearrangement, which are features of certain primary immunodeficiencies, ...19921327508
epidemiological and biological study of acquired immunodeficiency syndrome-related lymphoma in the county of los angeles: preliminary results.a population-based case control study of intermediate- and high-grade lymphoma in the county of los angeles, ca, was initiated in 1989. human immunodeficiency virus (hiv)-positive lymphoma patients are compared to hiv-negative lymphoma patients, to hiv-positive controls with acquired immunodeficiency syndrome but without lymphoma, and to hiv-positive asymptomatic individuals. the hiv-negative lymphoma cases are compared to neighborhood controls, who are matched in terms of age, sex, race/ethnici ...19921327509
epstein-barr virus-induced human b-cell lymphomas in scid mice reconstituted with human peripheral blood leukocytes.epstein-barr virus (ebv) infection is associated with immunoblastic b-cell lymphomas in immunosuppressed or human immunodeficiency virus-infected individuals and in scid mice reconstituted with human peripheral blood leukocytes (hu-pbl-scid) from ebv-seropositive donors. the risk of tumors appearing in the hu-pbl-scid mice differs among ebv-seropositive donors. four different outcomes have been noted: (a) no tumors appear (no incidence donors); (b) tumors appear in a fraction of hu-pbl-scid mice ...19921327510
inhibition of b cell proliferation by antisense dna to both alpha and beta forms of fc epsilon r ii.epstein-barr virus (ebv) infection activates b lymphocyte proliferation through partially understood mechanisms, resulting in phenotypic changes, including the appearance of new antigens. one such antigen is fc epsilon r ii/cd-23 which may be relevant for b cell proliferation. we have used anti-sense oligonucleotides to study the importance of the two forms of this molecule for proliferation in the ebv-transformed, fc epsilon r ii +ve lymphoblastoid b cell line, rpmi 8866. anti-sense oligodeoxyn ...19921327538
enrichment of igg anti-dna-producing lymphoblastoid cell lines by antigen-coated immunomagnetic this paper we describe the establishment of four anti-dna-producing lymphoblastoid cell lines (lcl) by epstein-barr virus (ebv) infection of peripheral blood b-cells from a patient with systemic lupus erythematosus. the lcl showed a heterogeneous cell composition: the frequencies of cells in active proliferation, cells secreting igg or igm, and cells effectively producing igg or igm anti-dna were estimated by limiting dilution analysis. the cells producing anti-dna antibodies were a small fra ...19921327598
detection of the epstein barr viral genome by an in situ hybridization method in salivary gland biopsies from patients with secondary sjögren's syndrome.sections of formalin-fixed paraffin-embedded minor labial salivary glands from 23 patients with sjögren's syndrome associated with rheumatoid arthritis (secondary sjögren's syndrome: sss) and from 11 patients with keratoconjunctivitis sicca (kcs) associated with rheumatoid arthritis but without proven ss were examined by in situ hybridization using the bamh1 v (w) fragment of epstein barr viral (ebv) dna. minor labial salivary glands from 7 healthy individuals were used as the control. ebv speci ...19921327603
induction of a brdu-enhanceable fragile site-like lesion and sister chromatid exchanges at 11q23.1 in ebv-transformed lymphoblastoid cell lines.we examined the expression of a fragile site-like lesion and induction of sister chromatid exchanges (sces) at 11q23.1 in ebv-transformed lymphoblastoid cell lines derived from carriers of distamycin a-inducible fragile sites and ataxia telangiectasia patients. the fragile site-like lesion at 11q23.1 was found to be brdu-enhanceable in all cell lines examined, and the expression frequencies increased linearly with the rates of brdu substitution in replicated dna. in addition, an increased freque ...19921327661
[the epstein-barr virus and aids-associated lymphomas of the central nervous system]. 19921327712
evidence of an in vitro association between human immunodeficiency virus antigen p24 and epstein-barr virus investigate the association between human immunodeficiency virus (hiv) and epstein-barr virus (ebv), simultaneous determinations of hiv antigen (hiv ag) p24 and ebv dna were performed in lymphocyte culture supernatants from 63 individuals at risk of hiv infection. in vitro data, together with hiv immune status results, were subjected to a statistical analysis. hiv infection was identified in 49 patients (78%); of these, in vitro ebv dna was found in 44 individuals (90%), while in only 3 of th ...19921327786
assessment of major histocompatibility complex class i interaction with epstein-barr virus and human immunodeficiency virus peptides by elevation of membrane h-2 and hla in peptide loading-deficient cells.earlier findings indicate that peptides can affect the expression of major histocompatibility complex (mhc) class i molecules on the surface of cells with defective peptide loading mechanism. we have used peptide induced increase of class i antigen expression to assess peptide interaction with mhc class i molecules. a panel of 41 overlapping synthetic peptides derived from the human immunodeficiency virus-1 (hiv-1) gag protein and 33 nonoverlapping peptides from epstein-barr virus (ebv) proteins ...19921327802
role of leucine residues in the c-terminal region of human interleukin-6 in the biological mutagenesis of two sets of three periodic leucine residues which appear at every seventh position in the c-terminal region of human interleukin-6 (il-6) was performed. both receptor-binding and immunoglobulin (ig)-induction activities of a triple mutant leu168,175,182-->val were only 1% compared with those of wild-type il-6. however, the mutant leu152,159,166-->val had 13% receptor-binding and 2% ig-induction activities of those of wild-type il-6. in order to obtain more direct inf ...19921327882
infectious agents in the etiology of esophageal cancer.extensive epidemiological and experimental studies have suggested that some chemical agents, nutritional deficiencies, and physical factors are associated with the development of esophageal cancer (ec). recent evidence also suggests an etiologic role of certain microorganisms in esophageal carcinogenesis either by producing carcinogens or promotors or by acting directly on the host cells. the mutagenic and carcinogenic effects of several fungi and bacteria isolated from the grains and foodstuffs ...19921327935
[experimental study of use of shuttle vector to develop a system of detecting mutagenesis in mammalian cells].the paper presents a series of experimental studies of the use of shuttle vector to develop a short-time mutagenesis system of detecting gene mutation in the mammalian cell at dna level. the system is based on the finding that in the eb virus the shuttle vector pmci5 carries laci gene. the laci is used as a target of induced mutagenesis in mouse cells. use pmci5 plasmid to transform the 3t3 mouse cell, expose it to a chemical mutagen, ethyl methanesulfonate (ems); transfer the treated plasmid dn ...19921328027
prevention of programmed cell death in burkitt lymphoma cell lines by bcl-2-dependent and -independent mechanisms.burkitt lymphoma (bl) cell lines which retain the phenotypic characteristics of the freshly-isolated tumour cells (group i cells) readily enter programmed cell death (apoptosis) in response to a variety of triggers. by contrast, isogenic bl cells which are phenotypically altered as a result of activation of their resident ebv genome (group-iii cells) are highly protected from apoptosis. phenotypic changes in group-iii cells include the up-regulation of the oncogene, bcl-2. expression of the 26-k ...19921328074
[the diagnostic value of epstein-barr virus diagnosis]. 19921328107
[new developments and prospects of antiviral chemotherapy]. 19921328109
inhibition of superoxide production in b lymphocytes by rac antisense oligonucleotides.rac1 and rac2 gene products are small gtp-binding proteins showing 92% homology to each other. according to recent studies performed in cell-free systems, rac1 and rac2 proteins may be involved in the activation of nadph-oxidase, the superoxide-generating enzymatic complex active in phagocytes. epstein-barr virus (ebv) transformed b lymphocytes, which express rac1 and rac2 genes, also efficiently release superoxide anions when triggered by various cell surface stimuli. to investigate the regulat ...19921328203
quantitative oropharyngeal epstein-barr virus shedding in renal and cardiac transplant recipients: relationship to immunosuppressive therapy, serologic responses, and the risk of posttransplant lymphoproliferative disorder.epstein-barr virus (ebv) infection plays a major role in the pathogenesis of posttransplant lymphoproliferative disorder (ptld). quantitative oropharyngeal ebv shedding measured by a dna-dna dot blot assay and the genotype of isolates determined by a polymerase chain reaction assay were studied in 23 renal and 23 cardiac transplant recipients followed over the first posttransplant year. five patients developed ptld and two additional ptld renal transplant recipients were studied from the time of ...19921328412
pseudomonal supraglottitis occurring in a patient with profound neutropenia secondary to virus-associated haemophagocytic syndrome.we present a case of virus-associated haemophagocytic syndrome following epstein-barr virus infection in which a fulminant pseudomonal supraglottitis developed. increasingly, unusual pathogens have been found in immunocompromised patients. this is the first reported case of pseudomonal supraglottitis.19921328436
analysis of the heterogeneity of the biological responses to native and mutant human interleukin-6.the structure-function relationships of the biological activities of mutant varieties of the pleiotropic cytokine interleukin-6 (human) were measured by three assays: induction of immunoglobulin m (igm) secretion from an epstein-barr virus-transformed human b cell line and induction of fibrinogen secretion from either a human hepatoma cell line or a rat hepatoma cell line. the biological effects of the cytokine were characterized by three parameters as determined by a novel analysis: effectivene ...19921328443
involvement of hla class i alleles in natural killer (nk) cell-specific functions: expression of hla-cw3 confers selective protection from lysis by alloreactive nk clones displaying a defined specificity (specificity 2).this study was designed to identify the target molecules of the natural killer (nk) cell-mediated recognition of normal allogeneic target cells. as previously shown, the gene(s) governing the first nk-defined allospecificity (specificity 1) were found to be localized in the major histocompatibility complex region between bf gene and hla-a. in addition, the analysis of a previously described family revealed that a donor (donor 81) was heterozygous for three distinct nk-defined allospecificities ( ...19921328466
alternative splicing determines the carboxy terminus of the epstein-barr virus nuclear antigen 5 species expressed in the burkitt's lymphoma cell line daudi.the daudi strain of epstein-barr virus (ebv) possesses a genomic deletion, relative to the b95-8 ebv prototype, that removes the entire epstein-barr virus nuclear antigen 2 (ebna2) open reading frame (orf) and the sequences encoding the carboxy terminus of ebna5. immunoblot analysis carried out in this study indicates that two species of ebna5 (31k and 37k) are expressed in daudi cells. nucleotide sequence analysis of daudi cdna clones has confirmed that, as a consequence of the genomic deletion ...19921328484
virological and pathological features of mice infected with murine gamma-herpesvirus 68.the primary infection of balb/c mice with murine herpesvirus 68 (mhv-68) was investigated. when the virus was introduced intranasally, the lung was the main tissue infected, the virus being associated with alveolar epithelium and mononuclear cells. a productive infection lasted for 10 days, after which viral dna could be detected by in situ hybridization up to 30 days after infection. at that time lymphoproliferative accumulations were also observed in the lung, with formation of germinal centre ...19921328491
stereotactic brain biopsy in the hope of finding a treatable condition, the need for rapid diagnosis in hiv-seropositive patients with brain lesions is apparent. in order to evaluate the efficacy of stereotactic brain biopsy in aids patients, we retrospectively studied 25 hiv-infected patients undergoing stereotactic biopsy. brain lesions were identified with gadolinium-enhanced mri and/or contrast ct. brain biopsy was performed using the system of riechert. from 8 up to 15 small tissue samples from one or two targets we ...19921328543
frequent expression of epstein-barr virus latent membrane protein-1 in tumour cells of hodgkin's disease in hiv-positive patients.epstein-barr virus (ebv) is believed to be implicated in the aetiology of non-hodgkin's lymphomas developing in immunodeficient individuals including aids patients. ebv has also been associated with hodgkin's disease (hd), where the genomes have been demonstrated in the hodgkin and reed-sternberg cells in some of the cases. recent evidence has shown that ebv genomes are transcribed in these cells, because the ebv-encoded latent membrane protein-1 (lmp-1) can be demonstrated in the tumour cells i ...19921328576
sleep, epstein-barr virus infection, musculoskeletal pain, and depressive symptoms in chronic fatigue syndrome.sleep physiology, viral serology and symptoms of 14 patients with chronic fatigue syndrome (cfs) were compared with 12 healthy controls. all patients described unrefreshing sleep and showed a prominent alpha electroencephalographic nonrapid eye movement (7.5-11.0 hz) sleep anomaly (p less than or equal to 0.001), but had no physiologic daytime sleepiness. there were no group differences in epstein-barr virus (ebv) antibody titers. the patient group had more fibrositis tender points (p less than ...19921328633
herpes simplex virus-infected human fibroblasts are resistant to and inhibit cytotoxic t-lymphocyte activity.we examined the ability of human anti-herpes simplex virus (hsv) cytotoxic t lymphocytes (ctl) to lyse autologous human fibroblasts infected with hsv. in contrast to hsv-infected human epstein-barr virus-transformed b cells (lcl), which were lysed by hla-restricted anti-hsv ctl, autologous fibroblasts infected with hsv were resistant to lysis. this resistance was not due to a lack of infectivity or production of hsv proteins since greater than 90% of the cells were infected and expressed abundan ...19921328666
the only domain which distinguishes epstein-barr virus latent membrane protein 2a (lmp2a) from lmp2b is dispensable for lymphocyte infection and growth transformation in vitro; lmp2a is therefore nonessential.using second-site homologous recombination, epstein-barr virus (ebv) recombinants were constructed which carry an lmp2a mutation terminating translation at codon 19. despite the absence of lmp2a or lmp2a cross-reactive protein, the recombinants were able to initiate and maintain primary b-lymphocyte growth transformation in vitro. ebna1, ebna2, and lmp1 expression was unaffected by the lmp2a mutation. the lmp2a mutant recombinant ebv-infected lymphoblastoid cell lines (lcls) were identical to wi ...19921328675
[epstein-barr virus (ebv): detection of ebv genomic sequences by polymerase chain reaction (pcr)]. 19921328710
incidental oral hairy leukoplakia in immunocompetent persons. a report of two this brief article we report on two hiv-negative patients with documented oral hairy leukoplakia who had no known risk factors for hiv infection nor any evidence of other forms of immune suppression. therefore we conclude that in some instances hairy leukoplakia can represent an isolated and innocuous epstein-barr virus infection.19921328983
[epstein-barr virus infection and immunoglobulin abnormalities in renal transplant recipients].the relationship between immunoglobulin abnormalities and ebv infection has been investigated in 65 renal transplant patients. immunoglobulins abnormalities were demonstrated in 44 (68%) patients, 8 of them (18%, 12% of all patients) had a monoclonal component. up to now no lymphoproliferative disorder has been observed in these patients. all patients were ebv seropositive at the beginning of the study and in 22 of them (33%) a reactivation of ebv infection could be demonstrated. no relation has ...19921329240
the suppressive effect of deoxyspergualin on the differentiation of human b lymphocytes maturing into immunoglobulin-producing cells.deoxyspergualin, an analog of spergualin, has been known as a novel immunosuppressive agent with strong immunosuppressive activity in in vivo experimental systems. in the present study, we examined the effect of deoxyspergualin (dsg) and methyldeoxyspergualin (medsg) on the proliferation and differentiation of human b lymphocytes in vitro. highly purified b cells from human tonsil samples were isolated by percoll density gradient from nonrosetted cells and were used as target cells. both agents ...19921329279
epstein-barr virus-associated t-cell lymphoma of the maxillary sinus in a renal transplant recipient. 19921329289
transcription of bamhi-a region of the ebv genome in npc tissues and b cells.analysis by northern blotting and sequencing of cdna clones from a transcription library of a tumor biopsy from a nasopharyngeal carcinoma (npc) patient showed that the bamhi-a region of the epstein-barr virus genome is abundantly and regularly transcribed in tumor tissues from npc patients. the transcription occurred in a rightward direction terminating between coordinates 160,965 and 160,995, where two polyadenylation sites are located. rightward transcription of this region also occurred in b ...19921329317
characterization of the murine cytomegalovirus genes encoding the major dna binding protein and the icp18.5 several herpesviruses the genes for the major dna binding protein (mdbp), a putative assembly protein, the glycoprotein b (gb), and the viral dna polymerase (pol) collocate. in murine cytomegalovirus (mcmv), two members of this gene block, pol (elliott, clark, jaquish, and spector, 1991, virology 185, 169-186) and gb (rapp, messerle, bühler, tannheimer, keil, and koszinowski, 1992, j. virol., 66, 4399-4406) have been characterized. here the two other mcmv genes are characterized, the gene enc ...19921329325
expression of epstein-barr virus membrane antigen gp350/220 in e. coli and in insect cells.the epstein-barr virus open reading frame bllf1 encodes the major envelope glycoproteins gp350 and gp220. fragments of the gp350/220 gene were expressed in escherichia coli in order to define regions of the polypeptide chain reacting with human sera. the c-terminal half of the protein was sufficient for recognition by all vca-positive sera tested. a membrane anchor truncated version of gp350/220 was expressed in insect cells using the baculovirus system. proteins of different sizes were specific ...19921329330
primary immunodeficiency diseases and epstein-barr virus-induced lymphoproliferative disorders.increased incidence of malignant disorders is noted in patients with both primary and acquired immunodeficiency diseases. the pathogenetic mechanism(s) for these disorders remain unclear. defective immunosurveillance of these patients, however, is mainly postulated to be responsible for the increased risk of these malignant disorders. of the malignant disorders, epstein-barr virus (ebv)-induced lymphoproliferative disorders (lpd) have been increasingly reported, possibly due to improved therapeu ...19921329432
adf (adult t cell leukemia-derived factor)/human thioredoxin and viral infection: possible new therapeutic approach.adf (adult t-cell leukemia-derived factor), originally defined as an inducer of interleukin 2 receptor/alpha (il-2r/alpha), is a homologue of thioredoxin. adf is constitutively produced by human lymphoid cell lines transformed by human t-lymphotropic virus type i (htlv-i) or epstein-barr virus (ebv). adf augments the proliferation of htlv-i and ebv transformed cells as an autocrine growth factor. these data are indicative of the possible involvement of adf in virus-related transformation of cell ...19921329444
painful diffuse osteosclerosis after intravenous drug abuse.we identify a new syndrome of acquired painful diffuse osteosclerosis associated with past intravenous drug abuse in two adults.19921329508
[epstein-barr virus associated lymphoproliferative syndrome after liver transplantation]. 19921329596
effect of ultraviolet irradiation on selected host cell proteins including ro/ss-a and epstein-barr virus in cultured lymphoblastoid cell lines.although systemic lupus erythematosus (sle) and sjögren's syndrome (ss) are distinct collagen vascular illnesses, they share certain features. both have clinical manifestations involving skin and mucous membranes and characteristically have high titers of circulating autoantibodies to the cellular components ro/ss-a, calreticulin/ro, 52 kda ro and la/ss-b. viruses have been postulated to be involved in the pathogenesis of both diseases. sensitivity to sun is a cardinal feature of sle, and uv lig ...19921329799
human b-lymphoid cell lines.the collective efforts during almost three decades by hematologists, tumor biologists and immunologists have provided a collection of established human hematopoietic cell lines, representing most of the hematopoietic cell lineages. the representativity of cell lines derived from the b cell differentiation lineage, however, is the most impressive. human b-lymphoid cell lines are extensively used world wide as models in studies of various aspects of b cell biology and as tools in research on the e ...19921329931
epstein-barr virus (ebv) and human hematopoietic cell lines: a review.two facts need to be pointed out to help explain why the history of epstein-barr virus (ebv) research has been inseparable from that of the studies with human hematopoietic cell lines of neoplastic and non-neoplastic origin. one is that burkitt lymphoma (bl) cell lines, ebv-positive or-negative, can be established in culture quite easily. thus, the bl cell lines which epstein established were indeed some of the first hematopoietic as well as virus-carrying cell lines of human neoplastic origin. ...19921329932
acute hepatitis in childhood: virological, immunological and clinical aspects.virological, immunological and clinical findings in 7 previously healthy children, aged 18 months to 11 years, with viral hepatitis are reported. asymptomatic and fully recovering, although protracted, hepatitis b was diagnosed by chance in a 1 1/2 year-old boy. anicteric and short-term hepatitis occurred in three children with epstein-barr virus infection, concomitantly with typical mononucleosis syndrome. on the contrary, cytomegalovirus (cmv)-associated hepatitis was severe and protracted in ...19921330050
viral infections.viral infections may cause serious morbidity as well as death in elderly patients. of the rna viruses, influenza virus is the most important pathogen; the majority of influenza-related deaths occur in older patients. respiratory syncytial virus appears to be gaining increasing importance in elderly persons. herpes zoster or shingles is caused by the dna virus, varicella-zoster virus, and its major morbidity in older patients is postherpetic neuralgia.19921330279
t cell recognition of epstein-barr virus associated lymphomas.epstein-barr virus, a lymphotropic herpesvirus of humans, has potent b cell growth transforming activity yet persists in the lymphoid tissues of most individuals as a lifelong asymptomatic infection. virus induced b cell growth transformation in vitro is associated with the expression of a limited set of viral genes encoding six nuclear antigens (ebna 1, 2, 3a, 3b, 3c and lp) and two latent membrane proteins (lmp 1, 2). healthy virus carriers possess strong ebv specific ctl memory that can be re ...19921330300
[iga antibodies against epstein-barr virus capsid antigen].the authors describe infection with the epstein-barr virus (ebv) which is associated with the formation of various types of antibodies--heterophil (hp) antibodies as well as antibodies against individual ebv antigens. they evaluate the contribution of a recently elaborated and introduced method used for detection of antibodies against the capsid antigen of the ebv class iga (anti-vca iga) by indirect immunofluorescence to the diagnosis of acute and chronic ebv infections. anti-vca iga are useful ...19921330312
herpesvirus serology, aberrant specific immunoglobulin g2 and g3 subclass patterns and gm allotypes in individuals with low levels of objective of this study was to determine whether igg3-deficient individuals have an increased frequency of reactivated herpesvirus infections. serum titres to epstein-barr virus (ebv) and human herpesvirus-6 were examined in 10 healthy and in 10 symptomatic persons with serum igg3 < 0.1 g/l. atypical titres were found in 16% of the igg3-deficient individuals. reactivations of these viruses thus do not seem common in igg3 deficiency. antigen-specific igg responses were also determined. a lowe ...19921330389
epstein-barr virus infection and associated diseases in children. i. pathogenesis, epidemiology and clinical aspects.epstein-barr virus (ebv), an ubiquitous human b lymphotropic virus, is the cause of infectious mononucleosis. moreover, ebv infection can be followed by lymphoproliferative diseases in patients with inherited and acquired immunodeficiencies. primary ebv infection may be a threat to all children after marrow or organ transplantation or those receiving chronic immunosuppressive treatment for various other reasons. the virus has been also implicated in the pathogenesis of different malignant tumour ...19921330572
in vivo production of interleukin-10 by malignant cells in aids lymphomas.expression of the interleukin (il)-10/bcrf1 gene was studied by in situ hybridization in tissue samples from acquired immunodeficiency syndrome (aids) lymphomas using a bcrf1 probe which also recognizes the human il-10 sequence. hybridization was detected in 8 out of 15 lymphomas. in contrast, the il-10/bcrf1 gene expression was detected in only 1 out of 11 lymphomas from human immunodeficiency virus (hiv)-seronegative patients (p = 0.05). in aids lymphomas, the number of cells labeled with a bc ...19921330578
chronic epstein-barr virus infection. 19921330851
molecular approaches to the study of human b-cell and (auto)antibody repertoire generation and selection.we have shown that the restricted repertoire of vh genes expressed in second trimester human fetal liver is not solely determined by jh proximity. furthermore, by following the fate of two vh gene segments in different b-cell repertoires, we have provided evidence that multiple factors contribute to the frequency with which individual vh genes are utilized. we found that the repertoire of adult blood igm-bearing b cells contains a high proportion of b lymphocytes that express extensively mutated ...19921330894
decreased expression of e-cadherin and increased invasive capacity in ebv-lmp-transfected human epithelial and murine adenocarcinoma cells.the ebv-encoded membrane protein lmp is one of 9 viral proteins regularly expressed in virally immortalized b lymphocytes. it is expressed in ebv-carrying lymphoblastoid cell lines of normal origin and in the majority of the poorly differentiated nasopharyngeal carcinomas, but not in burkitt lymphomas. lmp has been reported to transform rodent fibroblasts, to inhibit epithelial differentiation and to alter morphology and cytokeratin expression in an in vitro immortalized human keratinocyte cell- ...19921330938
high correlation between lipid peroxide radical and tumor-promoter effect: suppression of tumor promotion in the epstein-barr virus/b-lymphocyte system and scavenging of alkyl peroxide radicals by various vegetable extracts.we examined the ability of hot-water extracts of 66 vegetables and plants to suppress tumor promotion, as well as to scavenge lipid peroxide radicals in vitro. to assess the effect against tumor promotion (transformation) in vitro, we used the phorbol myristate acetate/epstein-barr virus/b-lymphocyte system. to assess the lipid radical-scavenging effect, the luminol-enhanced chemiluminescence method using the tert-butyl hydroperoxide/heme system was used, which generates more alkyl peroxide radi ...19921331005
structural constraints within a trimeric transcriptional regulatory region. constitutive and interferon-gamma-inducible expression of the hla-dra gene.constitutive and inducible transcription of the major histocompatibility class ii hla-dra gene involves the upstream s element and the conserved x and y elements. in this report we have addressed the roles of spatial constraints and stereospecific alignment between the upstream s and x elements, and the x and y elements, in both constitutive and interferon-gamma (gamma-ifn)-induced expression. analysis of the constitutive expression in b cell lines (b-lcl) has previously shown that the x and y e ...19921331098
generation of macaque b lymphoblastoid cell lines with simian epstein-barr-like viruses: transformation procedure, characterization of the cell lines and occurrence of simian foamy virus.two simian epstein-barr-like viruses, a rhesus epstein-barr virus and herpesvirus papio, were used to transform b cells from rhesus or cynomolgus macaques. the resulting cell lines exhibited predominantly a b lymphocyte phenotype and expressed epstein-barr virus antigens. the majority of b lymphoblastoid cell lines from macaques, which were seropositive for simian foamy virus, developed giant cells in culture. the cytopathic agent was identified as a foamy virus and was transmissible to human em ...19921331148
cloning and expression of a cdna encoding the epstein-barr virus thymidine kinase gene.a clone of the epstein-barr virus (ebv) thymidine kinase (tk) gene was derived from a cdna library of p3hr1 cells. the gene product was expressed as a fusion protein in a procaryotic system by using t7 rna polymerase. the recombinant tk showed a molecular mass of 67 kda and was biologically active. antiserum raised in mice immunized with partially purified tk recognized an antigen present in ebv-superinfected raji cells using an indirect immunofluorescence assay.19921331157
emerging human b cell repertoire. influence of developmental stage and interindividual b cell precursors developing in fetal lymphopoietic tissue, the selection of vh, dh, and jh gene segments for initial h chain gene assembly is biased. the present study was designed to determine whether these biases persist in fully developed human fetal b cells and to examine specificities encoded by the favored elements. b cells were prepared from two sites representing different stages of development, i.e., fetal liver as a source of cells newly generated in that lymphopoietic environment ...19921331240
the epstein-barr virus immediate-early promoter brlf1 can be activated by the cellular sp1 transcription factor.disruption of viral latency in epstein-barr virus-infected cells is mediated through the activation of the bzlf1 (z) immediate-early gene product. the z protein can be derived from either of two promoters: the bzlf1 promoter, which directs transcription of a 1.0-kb mrna encoding the z gene product alone, or the upstream brlf1 promoter, which directs transcription of a 2.8-kb bicistronic mrna encoding the brlf1 and bzlf1 immediate-early proteins. in this study we have examined the regulation of t ...19921331521
the epstein-barr virus (ebv) nuclear antigen 1 bamhi f promoter is activated on entry of ebv-transformed b cells into the lytic epstein-barr virus (ebv)-positive burkitt's lymphoma cell lines exhibiting the latency i form of infection (i.e., ebv nuclear antigen 1 [ebna1] positive in the absence of other latent proteins), the ebna1 mrna has a unique bamhi q/u/k splice structure and is expressed from a novel promoter, fp, located near the bamhi fq boundary. this contrasts with the situation in ebv-transformed lymphoblastoid cell lines (lcls) exhibiting the latency iii form of infection (i.e., positive for all latent pro ...19921331531
epstein-barr virus nuclear protein 2 is a critical determinant for tumor growth in scid mice and for transformation in vitro.injection of epstein-barr virus (ebv)-transformed human lymphoblastoid b cells into immunodeficient scid mice results in the appearance of rapidly growing, fatal human b-cell tumors. to evaluate the role of ebv nuclear protein 2 (ebna-2) in this process, we generated lymphoblastoid cell lines transformed by several ebv mutants which were identical except for deletions in the ebna-2 gene (j. i. cohen, f. wang, and e. kieff, j. virol. 65:2545-2554, 1991). these cell lines were injected intraperito ...19921331538
evolution of clonality and invasive behavior of epstein-barr virus immortalized lymphoblastoid cell lines in scid mice brains.recently established epstein-barr virus immortalized lymphoblastoid cell lines express polyclonal immunoglobulins, are diploid, and grow into invasive tumors when injected intracerebrally into mice with severe combined immunodeficiency (scid). it is unclear whether clonal selection of neurotropic cell lines occurs during long-term growth in the brain and the effect of this selection on brain invasiveness.19921331608
comparison of in situ hybridization using different nonisotopic probes for detection of epstein-barr virus in nasopharyngeal carcinoma and immunohistochemical correlation with anti-latent membrane protein antibody.the detection of epstein-barr virus (ebv) in nasopharyngeal carcinoma (npc) may be of diagnostic importance, particularly in cases from nonendemic areas. for cellular localization of viral genomes, cold in situ hybridization methods for the demonstration of ebv-associated npc remain difficult and relatively insensitive for routinely processed tissues.19921331609
expression of epstein-barr virus-encoded small rna (by the eber-1 gene) in liver specimens from transplant recipients with post-transplantation lymphoproliferative disease.epstein-barr virus (ebv)-associated post-transplantation lymphoproliferative disease (ptld) develops in 1 to 10 percent of transplant recipients, in whom it can be treated by a reduction in the level of immunosuppression. we postulated that the tissue expression of the small rna transcribed by the eber-1 gene during latent ebv infection would identify patients at risk for ptld.19921331789
epstein-barr virus: culprit or consort? 19921331791
hairy leukoplakia in an hiv-negative, nonimmunosuppressed patient.oral hairy leukoplakia is an epstein-barr virus-associated lesion that is considered to be a marker of immunosuppression. we report a case of oral hairy leukoplakia in a healthy nonimmunosuppressed elderly patient with no evidence of hiv infection on repeated investigation. this report has important implications on our understanding of the significance of this lesion.19921331928
cloning and characterization of the latent membrane protein (lmp) of a specific epstein-barr virus variant derived from the nasopharyngeal carcinoma in the taiwanese population.a dna fragment containing epstein-barr virus (ebv) terminal fragment sequence was obtained from a genomic library of nasopharyngeal carcinoma (npc). one of the clones (clone 1510) contained the gene encoding latent membrane protein (lmp). sequence analysis revealed that this gene had 95% homology with the lmp sequence of the b95-8 strain. among the sequence variations, there was a change from g to t at nucleotide position 169,426, resulting in the loss of an xhoi site in exon 1 of the lmp gene. ...19921331932
o2- production by b lymphocytes lacking the respiratory burst oxidase subunit p47phox after transfection with an expression vector containing a p47phox cdna.the respiratory burst oxidase of phagocytes and b lymphocytes is a complicated enzyme that catalyzes the one-electron reduction of oxygen by nadph. it is responsible for the o2- production that occurs when these cells are exposed to phorbol 12-myristate 13-acetate or other appropriate stimuli. the activity of this enzyme is greatly decreased or absent in patients with chronic granulomatous disease, an inherited disorder characterized by a severe defect in host defense against bacteria and fungi. ...19921332032
localization of epstein-barr virus-encoded rnas eber-1 and eber-2 in interphase and mitotic burkitt lymphoma cells.the subcellular distribution of the small epstein-barr virus-encoded rnas eber-1 and eber-2 has been investigated by using a high-resolution in situ hybridization technique. the distribution patterns in raji cells of fluorescent oligodeoxynucleotides complementary to each rna were detected by confocal laser scanning microscopy. both rnas were found in the cytoplasm as well as in the nuclei of interphase cells. in contrast, use of the same technique indicated an exclusively nuclear location for c ...19921332043
[epstein-barr virus; gene expression and replication]. 19921332122
clinical, histologic and genetic characterization of non-hodgkin's lymphomas in mexicans.we describe the anatomical distribution, histological and molecular characteristics of 32 cases of nhl. staging of the nhl was made according to conventionally accepted schemes. histologically the nhl were classified in grades following the criteria defined by the working formulation. rearrangements in one or more ig or tcr receptor genes were detected in southern blots and allowed us to determine the cell type and stage of differentiation. serological analysis of 26 serum samples revealed the e ...19921332169
purification and characterization of epstein-barr virus gp340/220 produced by a bovine papillomavirus virus expression vector system.our initial results with a bovine papilloma virus (bpv) vector expression system indicated that we could produce significant amounts of epstein-barr virus (ebv) gp340/220 in the supernatant of a mouse fibroblast cell line. we have now extended these findings to show that the truncated version of gp340/220, where the membrane anchor sequence is deleted, is produced even after extended passage of the cells, at a level of approximately 1 mg/4 x 10(8) cells. a simple purification protocol using seph ...19921332270
the etiology and prevention of aerodigestive tract cancers. 19921332445
characterization of hu-pbl-scid mice with high human immunoglobulin serum levels and graft-versus-host disease.a chimeric model consisting of severe combined immune deficiency (scid) mice populated with human peripheral blood leukocytes (pbl) has recently been described (bu-pbl-scid mice). these reports indicated a limited reconstruction of the transferred human immune system and functionality of the human graft. herein we described modifications of the pbl transfer method that minimize transfer time and cellular manipulations, leading to a more effective population of scid mouse recipients. severe combi ...19921332484
quantitation of polymerase chain reaction products by hybridization-based assays with fluorescent, colorimetric, or chemiluminescent this report two nonradioactive assays for quantitative analysis of polymerase chain reaction (pcr) products are presented. in the first assay, magnetic beads coated with streptavidin were used to capture biotinylated pcr fragments. after hybridization with a hapten-labeled probe, these beads were analyzed either by flow cytometry (method a) or by immunoenzymatic reactions (method b). using a dilution series of purified pcr products, we consistently found a lower detection limit of 1.5 fmol fo ...19921332534
the epstein-barr virus lmp1 gene product induces a20 zinc finger protein expression by activating nuclear factor kappa b.a20 is an inducible zinc finger protein that confers resistance to tumor necrosis factor alpha cytotoxicity. a survey of various cell lines revealed that a20 was constitutively expressed in epstein-barr virus (ebv)-immortalized b-cells. transfection experiments demonstrated that the ebv latent membrane protein lmp1 induced a20 expression. lmp1 is a transforming protein of ebv that has dramatic effects on cell growth, activation, and survival. an integral membrane phosphoprotein, lmp1 bears no ho ...19921332946
association of epstein-barr virus infection and pulmonary exacerbations in patients with cystic fibrosis.we observed severe pulmonary exacerbations during primary epstein-barr virus (ebv) infection in adolescent patients with cystic fibrosis. since ebv is not a known respiratory tract pathogen in cystic fibrosis, we studied retrospectively all ebv-susceptible patients ages 6 to 18 years with chronic pseudomonas respiratory tract colonization hospitalized for a pulmonary exacerbation during an 18-month period. patients with serologic evidence of primary ebv infection (n = 5) were compared to control ...19921333067
the interleukin-10 homolog encoded by epstein-barr virus enhances the reactivation of virus-specific cytotoxic t cell and hla-unrestricted killer cell responses.we determined what influence the epstein-barr virus (ebv)-encoded homologue of il-10 (viral or vil-10) had on immune responses important for the control of ebv infection. we produced recombinant vil-10 in a b cell line. a 17-kda recombinant protein was secreted and had the same molecular weight as vil-10 secreted by ebv-infected b cells. functional activity of recombinant vil-10 was shown by the inhibition of interferon-gamma production by activated leukocytes. cytotoxic t cells and hla-unrestri ...19921333123
[contribution of immunohistochemistry and in situ hybridization techniques in diseases caused by or associated with epstein-barr virus].epstein-barr virus is associated with many diseases. today, the pathologist may study either by immunohistochemistry or in situ hybridization on tissue sections: ebv genome, ebv messenger rna, ebv latent and replicative proteins. several technics can be performed on fixed paraffin-embedded tissue to demonstrate the presence of ebv dna, eber-1 rna, lmp-1 protein. frozen tissues are required for the study of ebna-2, zebra and replicating proteins expression. the results, obtained during the study ...19921333177
gene expression in single reed sternberg cells of hodgkin's disease: results from pcr generated single cell cdna libraries. 19921333268
hodgkin's disease and epstein-barr virus.seroepidemiological and molecular biological studies have established an association of hodgkin's disease with epstein-barr virus (ebv). recently, ebv genomes and gene products, most notably the latent membrane protein (lmp), have been detected in approximately 50% of the cases. the findings suggest that ebv may contribute to the pathoetiology of a sizable proportion of hodgkin's disease cases.19921333269
biology of hodgkin's disease.the biology of hodgkin's disease is one of the most intriguing subjects in lymphoma research. the presence of only a small proportion of neoplastic cells and a vast majority of reactive cells reflects the presence of complex interactions between these cell types. in this paper we discuss findings indicating that reed-sternberg cells may be virally transformed cells that have evaded a cytotoxic immune response and induce an ineffective delayed type hypersensitivity reaction.19921333271
oncogenes in hodgkin's disease.the following manuscript reviews data presented at the cologne meeting relating to oncogene expression in hodgkin's disease. presented data ranged from investigations of oncogene expression in cell lines, where transcripts of unique size were identified and lineage related expressions of transcription factors described to detailed cytogenetic investigations of fresh hodgkin's biopsy tissue. particular attention was centred on discrepancies in the described expression of t(14; 18) and the molecul ...19921333273
hepatitis in pre-school children: prevalent role of cytomegalovirus.virological and serological investigations were performed on 8 children with clinical and/or laboratory signs of hepatitis. cytomegalovirus (cmv) appeared as the most frequently involved etiologic agent, since it was associated with 5 severe or chronically-evolving cases. out of the other 3 patients with non-cmv associated hepatitis, all completely recovering, two had clinically typical epstein-barr virus infections, while the remaining patient had an asymptomatic hbv infection.19921333326
midline malignant b-cell lymphoma with leukemic transformation.this article presents a case of lethal midline granuloma on the palate of a 44-year-old woman, which had been identified histologically as b-cell lymphoma with leukemic transformation in the terminal stages. at the first visit, physical and laboratory examinations showed no remarkable findings except for a necrotizing ulcer of the palate, and the biopsy specimens only showed massive inflammatory cell infiltration and necrosis of the granulation tissue. there was a short-term resolution after tre ...19921333355
chediak-higashi lymphoblastoid cell lines: granule characteristics and expression of lysosome-associated membrane proteins.chediak-higashi syndrome (chs) is characterized morphologically by the presence of giant lysosomal granules resulting from the dysregulated fusion of primary lysosomes. lysosome-associated membrane proteins comprise a family of highly glycosylated proteins which are postulated to facilitate many aspects of normal lysosomal function. in this study, epstein-barr virus-transformed lymphoblastoid cell lines derived from a patient with chs were analyzed for the presence of giant granules and the expr ...19921333377
human ebv-transformed lymphocytes of patients with schistosoma japonicum infection secrete idiotypically related immunoregulatory antibodies.lymphocytes derived from the peripheral blood of individuals infected with schistosoma japonica were transformed in vitro with ebstein-barr virus (ebv). serological characterization of antibody molecules revealed both antigen reactive (idiotypic) and anti-idiotypic transformants. one idiotypic ebv transformant, lo2c2, describes a major cross-reactive idiotype associated with anti-antigen binding molecules. other antibody populations expressing idiotypic cross-reactivity were derived from separat ...19921333380
detection and quantification of latently infected b lymphocytes in epstein-barr virus-seropositive, healthy individuals by polymerase chain reaction.we designed a highly sensitive and specific polymerase chain reaction assay for the detection of epstein-barr virus (ebv)-related sequences in b-cell dna of ebv-seropositive healthy individuals. by using this assay, we were able to amplify at least 10 copies of a plasmid containing the bamhi-w region, which is repeated up to 11 times within the ebv genome, in the presence of 1 microgram of ebv-negative dna, indicating that one virus genome was detectable from 150,000 cells. in 15 of 16 tested in ...19921333480
[hiv cofactors in the course of aids].the responsibility of hiv in the occurrence of aids is definitely established, but "cofactors" are strongly suspected to intervene, which would explain the difference courses followed by the disease in one patient or the other. some of these cofactors are related to the host or result from his behaviour. thus, age at the time of hiv acquisition and the patient's hla group are associated with differences in the speed of progression towards aids. attitudes that lead to reexposure to the virus by t ...19921333599
enteropathy-associated t-cell lymphoma in a renal transplant patient with evidence of epstein-barr virus involvement.the clinical and histological findings in a 54-year-old patient with enteropathy-associated t-cell lymphoma (eatl) occurring 18 years after renal transplantation are presented. ten years after adult-onset coeliac disease the patient developed medium to large t-cell non-hodgkin's lymphoma of the small intestine. epstein-barr virus (ebv) genome was detected by polymerase chain reaction in the lymphoma tissue and localized via epstein-barr virus rnas in situ hybridization to some of the tumour cell ...19921333679
epstein-barr virus in waldeyer's lymphatic tissue. 19921333723
tonsils: the gatekeeper of mucosal immunity? 19921333724
identification of a protein encoded in the eb-viral open reading frame bmrf2.using monospecific rabbit sera against a peptide derived from a potential antigenic region of the epstein-barr viral amino acid sequence encoded in the open reading frame bmrf2 we could identify a protein-complex of 53/55 kda in chemically induced b95-8, p3hr1 and raji cell lines. this protein could be shown to be membrane-associated, as predicted by previous computer analysis of the secondary structure and hydrophilicity pattern, and may be a member of ebv-induced membrane proteins in lytically ...19921333758
infectivity determinants encoded in a conserved gene block of human herpesvirus-6.the nucleotide sequence was determined for a 9.3 kb bamhi dna fragment derived from a cosmid clone (lorist 6) library of the 160 kb human herpesvirus-6 (hhv-6) strain u1102 genome. analysis of the sequence showed two different sources for the dna; 8.0 kb was derived from hhv-6, while 1.3 kb was derived from the right repeat of transposon tn10, the insertion sequence (is) element is10r. the is element sequence is shown to be derived from the host bacteria of the plasmid. the hhv-6 sequence repres ...19921333836
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