Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| long-term t-cell-mediated immunity to epstein-barr virus in man. i. complete regression of virus-induced transformation in cultures of seropositive donor leukocytes. | peripheral blood mononuclear cells from donors of known serological status with respect to eb virus were exposed to the virus in vitro and then cultured at various cell concentrations. all cultures from nine seronegative adult and 12 foetal donors gave rise to cell lines following subculture 4 weeks post infection. in contrast, seropositive donor cultures seeded at the higher cell concentrations developed foci of proliferating ebna-positive cells within the first 1--2 weeks but thereafter regres ... | 1978 | 214405 |
| malignant lymphoma in four of five siblings. | in one family four out of five siblings were affected with malignant lymphoma and hd has been diagnosed in two other relatives. hla-haplotypes were identical for the three diseased siblings who were so studied, whereas for the unaffected one they were different. | 1978 | 214406 |
| in vitro effects of epstein-barr virus on peripheral blood mononuclear cells from patients with rheumatoid arthritis and normal subjects. | peripheral blood mononuclear cells from 10 patients with rheumatoid arthritis and 9 control subjects were cultured in vitro for 30 days with and without infection by epstein-barr virus. all cultures showed polyclonal stimulation of b cells as indicated by rising levels of igm in the culture supernates, reaching maximal at 18-24 days, and with no quantitative or kinetic difference between the ra and control cells. igm anti-igg was also produced in both groups and maximally at 18-24 days, but in g ... | 1978 | 214511 |
| antibody-dependent cellular cytotoxicity against epstein-barr virus-associated antigens in african patients with nasopharyngeal carcinoma. | antibody-dependent cellular cytotoxicity (adcc) was performed on serum samples from african patients with nasopharyngeal carcinoma (npc) to determine whether antibody titers determined by this assay might be of prognostic value in this disease. the serum donors were divided into two groups: (1) those individuals who died within 2 years following diagnosis of npc; and (2) individuals who responded well to therapy and surivived for more than 2 years following diagnosis. the adcc gmt for the surviv ... | 1978 | 212366 |
| electron microscopic studies of tumor viruses. ii. entry and uncoating of epstein-barr virus. | entry of epstein-barr virus into human lymphoblastoid cells (daudi cells) was studied by electron microscopy. at the site of viral attachment, two distinct interactions conducive to penetration of the virus occurred between the viral envelope and cell membrane, namely, (i) simultaneous dissolution of both the envelope and cell membrane, presumably resulting in passage of viral capsids into the cytoplasm and (ii) dissolution confined to the cell membrane with resulting penetration of enveloped vi ... | 1978 | 214669 |
| antibody patterns to herpesviruses in kaposi's sarcoma. ii. serological association of american kaposi's sarcoma with cytomegalovirus. | the prominent finding of this extended serologic analysis on american and african kaposi's sarcoma (ks) patients and appropriately matched control groups is the detection of a specific serologic association of cytomegalovirus (cmv) with american ks patients. all american ks sera contained cmv antibodies and their geometric mean titers (gmt) were significantly higher than those in sera of melanoma patients (gmt ratio k = 5.3 to 7.7 by complement fixation [cf], k = 8.9 by indirect hemagglutination ... | 1978 | 212367 |
| production of migration inhibitory factor (mif) by human leukocytes following exposure to epstein-barr virus. | 1978 | 208782 | |
| stimulation of peripheral human lymphocytes by autologous ebv genome-carrying lymphoblastoid cell lines. | 1978 | 208783 | |
| characterization of a unique cell line (laz 221) from human acute lymphocytic ("null" cell) leukemia. | a unique human cell line designated laz 221 has been established from the peripheral blood of a patient with acute lymphocytic leukemia of the "null" cell type. the cell line does not possess the epstein-barr virus nuclear antigen and has a karyotype of 45,xx,-9,-12,+(9q12q). both the established cell line and the patient's uncultured blast cells share the same phenotypic markers. they both lack t-cell markers. they fail to form sheep erythrocyte rosettes and do not react with t-cell-specific an ... | 1978 | 205365 |
| primary epstein-barr virus infections in african infants. i. decline of maternal antibodies and time of infection. | 1978 | 212369 | |
| primary epstein-barr virus infections in african infants. ii. clinical and serological observations during seroconversion. | 1978 | 212370 | |
| serological reactivities of human and baboon sera against ebna and herpesvirus papio-determined nuclear antigen (hupna). | 1978 | 213288 | |
| lysosomal acid hydrolases in established lymphoblastoid cell lines, transformed by epstein-barr virus, from patients with genetic lysosomal storage diseases. | lysosomal acid hydrolases were determined in established lymphoblastoid cell lines, transformed in vitro by epstein-barr virus (ebv) from lymphocyte-rich cell populations isolated from the peripheral blood of patients with genetic lysosomal storage diseases--hurler syndrome, scheie syndrome, gm1-gangliosidosis type 1 and type 2, tay-sachs disease, and i-cell disease--and from obligate heterozygotes for these diseases. the respective enzyme activity was undectectable in lymphoblastoid cells from ... | 1978 | 213379 |
| interaction of the b95-8 and p3hr-1 substrains of epstein-barr virus (ebv) with peripheral human lymphocytes. | two substrains of the epstein-barr virus derived from the b95-8 and p3hr-1 cell lines were studied for their interaction with human peripheral lymphocytes. it has been previously shown that b95-8 virus has and p3hr-1 virus lacks lymphocyte-transforming ("immortalizing") properties. dna stimulation induced by b95-8 virus showed a good correlation with the number of surface ig-positive cells. p3hr-1 virus added before b95-8 virus completely abolished the stimulation of dna synthesis. it also preve ... | 1978 | 212371 |
| a greatly simplified method of establishing b-lymphoblastoid cell lines. | ten lymphoblastoid cell lines were established by epstein-barr virus-induced transformation directly from 0.04 to 0.15 ml of peripheral whole blood of one patient with xeroderma pigmentosum and four normal healthy adults. all these lines expressed b-lymphocyte characteristics. the advantages of this method are: (a) only a few drops of blood are required for establishing a permanent line; (b) damage and loss of cells in separation procedures are minimal; and (c) the method is simple, reliable, an ... | 1978 | 210942 |
| presence of ebna in nasopharyngeal carcinoma and control patient tissues related to ebv serology. | 1978 | 212372 | |
| recovery of transforming ebv from non-producer cells after superinfection with non-transforming p3hr-1 ebv. | cells of the raji and nc37 lines can be induced by chemical inducers, such as brdurd and idurd, or the tumor-promoter tpa to ea-expression only, but do not reveal any vca synthesis. after superinfection by nontransforming p3hr-1 ebv, however, a varying percentage of the cell population shows vca synthesis and releases infectious viral particles. the recovered virus differs biologically from p3hr-1 ebv since it transforms human umbilical cord blood lymphocytes into ebna-positive lymphoblastoid ce ... | 1978 | 212377 |
| [virus diseases of the lymphatic system. differential diagnosis and therapy]. | 1978 | 212661 | |
| two epstein-barr virus-associated dna polymerase activities. | we have partially purified and characterized two separate dna polymerase activities associated with epstein-barr virus (eb virus). one activity is present in eb virus producer cell lines but not in nonproducer or negative cell lines. it adheres more strongly to deae-cellulose than any host cell enzymes, eluting at 210 to 270 mm potassium phosphate buffer. further elution from phosphocellulose and sedimentation in glycerol gradients yields an enzyme purified 900-fold with an s value of 8.3. the s ... | 1978 | 213439 |
| activation of endogenous type c virus in balb/c mouse cells by herpesvirus dna. | several virion and nonvirion dnas were tested for the ability to activate endogenous type c virus in balb/c-derived mouse cells using the calcium precipitation technique. the dnas from all herpesviruses tested activated xenotropic type c virus synthesis. these included dnas from herpes simplex virus types 1 and 2, epstein-barr virus, human cytomegalovirus, sa8 virus, infectious bovine rhinotracheitis virus, pseudorabies virus, and herpes saimiri virus (m-dna). in contrast, dnas from vaccinia vir ... | 1978 | 212761 |
| expression of latent epstein-barr virus genomes in human epithelial/burkitt's lymphoblastoid hybrid cells. | expression of latent epstein-barr virus genomes in somatic-cell hybrids of burkitt's lymphoblastoid cells has been studied. treatment of the hybrid cells, d98/raji and d98/hr-1, with iudr induced the formation of ea and vca and replication of virus dna, whereas the same treatment of raji cells induced only the formation of ea. the patterns of transcription of virus genomes in these three cell lines were, however, very similar: 25% without treatment with iudr, 30% immediately after the treatment ... | 1978 | 215523 |
| interaction between epstein-barr virus and type-c virus in human cells. | the interaction between ebv and type-c viruses was studied in our fvnc experimental system, in which ebv and type-c viral genomes are contained in each cell. the data indicate that the human lymphoid fvnc cells are sensitive to both ebv and type-c virus exposure, showing high frequencies of induction of both repressed viral genomes. the two different viral genomes may be associated with different chromosomes in individual cells. | 1978 | 215524 |
| epstein-barr virus-related serology in nasopharyngeal carcinoma and controls. | 1978 | 215525 | |
| clinical evaluation of ebv serology in american patients with nasopharyngeal carcinoma. | there is now extensive immunological, biological and biochemical evidence to support a possible etiological relationship between ebv and npc in patients from different geographical locations. besides providing information on the question of etiology, the results from immunological investigations suggest that antibodies to some of the ebv-associated antigens might also be of clinical importance in the diagnosis and prognosis of npc. to determine the possible clinical application of ebv serology t ... | 1978 | 215526 |
| the genetic and antigenic basis for the iga antibody response to epstein-barr viral capsid antigen. | 1978 | 215527 | |
| presence of epstein-barr virus specific iga in saliva of nasopharyngeal carcinoma patients: their activity, origin and possible clinical value. | 1978 | 215528 | |
| [isolation of the epstein-barr virus in suspension cell lines obtained from persons with different forms of hemoblastosis]. | isolation of ebv from patients with different types of haemoblastosis by establishing virus-producing cell lines is described. ebv was isolated from patients with myelogenous leukemia in 71%, hodgkin's disease--in 22%. ebv was also isolated from 1 patient with lung tumor and leukemogenic reaction. all 8 established cell lines produced ebv in 0.5--3% of cells. the cells of the lines concerned are lymphoblasts and show b-cell characteristics. | 1978 | 212872 |
| cultural, morphological, cell membrane, enzymatic, and neoplastic properties of cell lines derived from a hodgkin's disease lymph node. | a neoplastic cell line (designated hut11) has been established in continuous culture from an involved lymph node of a patient with stage iia hodgkin's disease of the mixed cellularity type. the hut11 line has been morphologically heterogeneous, consisting of mononucleate lymphoid-like cells, polygonal epithelioid cells, and mono-, bi-, and multinucleate giant cells. four clones initiated from isolated binucleate giant cells of the hut11 line also have been successfully established as continuous ... | 1978 | 209894 |
| detection of epstein-barr virus in biopsied malignant lymphoma cell and its continuous culture. | in more than 80% of tumor cells in the pericardiac effusion of a case of malignant b-cell lymphoma, epstein-barr virus-determined nuclear antigen (ebna) was detected by the anticomplement immunofluorescence test. moreover, herpestype virus particles, although few in number, were demonstrated in the nucleus of lymphoma cells by an electron microscope. tumor cells in the pericardiac effusion were seeded at 98% purity after centrifugation on ficoll-conray and, soon after plating, they proliferated ... | 1978 | 210075 |
| epstein-barr virus in somatic cell hybrids between mouse cells and human nasopharyngeal carcinoma cells. | somatic cell hybrids between mouse cells and cells derived directly from npc biopsies were produced in order to study the association of the epstein-barr virus (ebv) genome and the expression of epstein-barr nuclear antigen (ebna) with the human chromosome(s). all attempts to correlate the presence of ebv-dna and the expression of ebna with the presence of a particular human chromosome(s) showed that the segregation of ebv-dna or of ebna and human chromosomes was dysconcordant. the data, therefo ... | 1978 | 213442 |
| in vivo cell-mediated immunity in chinese patients with nasopharyngeal carcinoma. | delayed hypersensitivity to antigens derived from four ebv-related (khly 28, raji, f265 and nc37) and one ebv-unrelated (molt) t-lymphoid cell lines and to standard antigens (trichophyton, candida albicans and streptokinase/streptodornase) was measured in 104 npc patients and 24 patients with other cancers. of the npc patients with non-disseminated disease, 55/86 (63.2%) had a positive skin reaction to hkly 28 extract, compared with only 1/18 (5.6%) oc patients with non-disseminated disease. the ... | 1978 | 215533 |
| use of immunological studies in evaluating the clinical course of nasopharyngeal carcinoma. | the prognosis of a npc patient is influenced by many factors, including the therapeutic method used and the clinical stage and histological characteristics of the tumour. the immune response of the peripheral lymphocytes was examined in 27 patients with npc, and it was concluded that this response accurately indicates the clinical course of the diseases as compared with clinical stage of disease, histological type of tumour and anti-vca-antibody titre. | 1978 | 215534 |
| nasopharyngeal carcinoma: etiology and control. recommendations. | 1978 | 215535 | |
| animal models for nasopharyngeal carcinoma. | 1978 | 215536 | |
| intracisternal tubular inclusions in nasopharyngeal carcinoma. | biopsy specimens obtained from 20 cases of nasopharyngeal carcinoma were examined under electronmicroscope for the presence of intracisternal tubular inclusions (iti). in eight (40 per cent) of them these inclusions could be identified. although the morphogenesis and significance of these iti are not clearly understood, they have been found in several autoimmune and viral diseases, including infectious mononucleosis, a disease caused by ebv, and burkitt's lymphoma, which is closely associated wi ... | 1978 | 213517 |
| detection of b-lymphocyte (b-cell)-associated antigens on human leukemic lymphocytes. masking of membrane antigens. | an indirect immunofluorescent technic has been used for rabbit antisera (anti-ep) that demonstrated complement-dependent cytotoxicity against human leukemic lymphocytes but not against normal blood lymphocytes. with the immunofluorescent technic, the antisera were found to react with 2--23% of normal blood lymphocytes. simultaneous staining of normal cells with anti-ep and for surface immunoglobulins (sig) on bone-marrow-derived b-cells showed that the proportions of stained cells were similar t ... | 1978 | 213964 |
| tumor viruses and human cancer. ii. dna tumor viruses. | 1978 | 214057 | |
| [early granuloma formation, a rise in eb virus antibody titer with an interesting ultrastructural observation and reticulum cell sarcoma-like pattern--an autopsy case]. | 1978 | 215801 | |
| ebna antibodies are more useful than complement fixing antibodies in monitoring infectious mononucleosis patients. | thirty-six patients with infectious mononucleosis (im) were followed prospectively for both anti-epstein-barr virus nuclear antigen (ebna) and complement-fixing antibodies using the soluble ebv antigen (cf/s). discrepancies in time between the two reactivities were found, due to the differences in sensitivity of the tests. it is suggested that, in clinical virology laboratories, ebna and not cf/s tests be used. | 1978 | 214180 |
| [recent data on the pathogenic role of the epstein-barr virus]. | 1978 | 215968 | |
| dna of epstein-barr virus. iv. linkage map of restriction enzyme fragments of the b95-8 and w91 strains of epstein-barr virus. | the arrangement of ecori, hsu i, and sal i restriction enzyme sites in the dna of the b95-8 and w91 isolates of epstein-barr virus (ebv) has been determined from the size of the single-enzyme-cleaved fragments and from blot hybridizations that identify which fragments cut from the dna with one enzyme contain nucleotide sequences in common with fragments cut from the dna with a second enzyme. the dna of the b95-8 isolate was the prototype for this study. the data indicate that (i) approximately ... | 1978 | 214576 |
| antigenic content of an in vitro cellular immunity reactive cell surface extract of raji cells. | antigenic content of a raji cell surface extract (ts) was studied by sequential abosrption of rabbit antiserum to this extract with different cells. anti-ts was first allowed to react exhaustively with human blood cells followed by hela cells and human diploid fibroblasts. the residual reactivity was studied by stepwise absorption with an ebv-negative b lymphoblastoid cell line, bjab, and then with b1--19, a clone derived from ebv transformed bjab cells. these processes delineated three kinds of ... | 1978 | 215106 |
| a study of epstein barr virus receptor activity in cell free extracts of human lymphoblastoid cells. | epstein barr virus (ebv) receptor activity in cell free extracts is operationally defined as one which causes a reduction in the effective concentration of the early antigen inducing particles of an ebv preparation when the latter is preincubated with the extract before infection. such activity was detected in the surface extract of raji cells and to a lesser extent in that of bjab cells, both of which are b lymphoblastoid cells that are susceptible to infection with ebv. receptor activity was n ... | 1978 | 215107 |
| epstein-barr virus early antigen titer by immunofluorescence in microplates. a new semi-automated method based on microtiter system. | for the titration of antibodies directed against epstein-barr virus early antigen (ebv-ea) we describe a method in microplates with the microtiter system (cooke engineering co., alexandria, va). using this technique, which allows rapid epidemiological investigations, we have titered anti-ebv-ea antibodies in different groups of patients and controls, and particularly in group which had recent contact with infectious mononucleosis. in this group the percentage of individuals having antibodies dir ... | 1978 | 214682 |
| complete heart block in epstein-barr myocarditis. | 1978 | 214746 | |
| epstein-barr virus-epithelial cell interaction and its implication in the etiology of nasopharyngeal carcinoma. | the etiological role of ebv in npc is still a matter for debate. a major question is how the viral dna becomes associated with the carcinoma cell, since only b lymphocytes are at present known to have receptors to ebv. the following hypotheses are proposed: (1) the epithelial cells of the nasopharynx have ebv receptors in vivo; transformation of the epithelial cell thus results from a direct interaction between ebv and this cell type. the epithelial cells of the nasopharynx are not permissive fo ... | 1978 | 215520 |
| early and late components of epstein-barr virus-associated membrane antigen in superinfected daudi cells and their reactivity with sera from nasopharyngeal carcinoma patients. | investigations were carried out on newly synthesized ma, vca and ea in daudi cells superinfected with the p3hr-1 strain of ebv and treated with trypsin to remove previously adsorbed ma-positive material from the cell surface. synthesis of ma, vca and ea was completely blocked by puromycin. a marked reduction in the frequency of ma-positive cells was observed in the infected cell cultures in the presence of either ara-c or pa, but a fraction of the ma-positive cells was insensitive to the inhibit ... | 1978 | 215521 |
| heterogeneity of epstein-barr virus derived from p3hr-1 cells. | infection of cells of the ebv-free human b-lymphoma lines bjab and ramos resulted in conversion of these cells to ebv-genome carriers expressing ebna. ebv isolates from p3hr-1 cells induced a heterogeneous ebna pattern: both a faintly granular pattern and brilliant ebna-expression were observed. the two types of ebna-expressing cells could be separated upon cloning. brilliantly ebna-expressing cells always segregated varying percentages of ebna-negative cells. an ebna-negative subclone derived f ... | 1978 | 215522 |
| differences in ebv antibody titres of patients with nasopharyngeal carcinoma originating from high, intermediate and low incidence areas. | in order to assess the differences in serological reactivities of npc patients from high, intermediate and low incidence areas, 288 npc sera from hong kong, singapore, tunis, east africa, paris and los angeles, together with sera from patients with ear, nose and throat tumours other than npc and with those from normal individuals from the same areas, were tested 'blind' with the same batches of antigen. important differences (up to 3-fold) in the gmts of antibodies directed against vca, ea and e ... | 1978 | 215529 |
| cell-mediated immunity, epstein-barr virus and nasopharyngeal carcinoma. | cellular immunity is apparently important in host defenses against cancer. a growing body of information on cmi to ebv is here reviewed, providing a strong basis for studies of such reactivity in npc. present work suggests that cmi responses to virus- and tumour-associated antigens may have implications for etiology, diagnosis and treatment. | 1978 | 215530 |
| general immunological status of nasopharyngeal carcinoma patients in singapore. | newly diagnosed npc patients were found to have impaired general t-cell functions, as determined in vivo by the mantoux test and in vitro by the pha response assay. treated remission patients were as hyporesponsiveness as newly diagnosed patients. pha hyporesponsiveness was associated with the hla profile of a2-b sin 2 and with high antibody titres to ebv-related antigens, in particular to the early antigen. impaired responses in the mantoux or pha tests were associated with poor survival. the i ... | 1978 | 215531 |
| epstein-barr virus and human chromosomes: close association of the resident viral genome and the expression of the virus-determined nuclear antigen (ebna) with the presence of chromosome 14 in human-mouse hybrid cells. | fourteen hybrid clones derived from the fused cultures of human lymphoblastoid fv5 cells and 5-bromodeoxyuridine-resistant mouse fibroblastic mcb2 cells grown in hypoxanthine/aminopterin/thymidine selective medium were examined for the presence of epstein-barr virus (ebv) dna, the expression of the virus-determined nuclear antigen (ebna), and the presence of human chromosomes, in the course of serial passage in vitro. among the hybrid clones tested, 3 were positive for ebv dna and ebna, whereas ... | 1978 | 217014 |
| infectious mononucleosis and related syndromes. | the "mono syndrome" is an acute febrile disease of older children and young adults which involves the lymphatic system and is characterized hematologically by the presence of 50% or more lymphocytes and monocytes and 10% or more atypical lymphocytes. epstein-barr virus (ebv) causes over 90% of the syndrome, cytomegalovirus (cmv) about 5% to 7%, and toxoplasma gondii less than 1%. viral hepatitis, adenovirus, rubella, and herpes simplex are rare causes. only ebv produces classical heterophile-pos ... | 1978 | 217270 |
| autoantibodies (cold lymphocytotoxins, antiactin antibodies and antinuclear factors) in nasopharyngeal carcinoma patients. | lta, sma and ana were found at higher frequencies in sera from npc patients than in those from matched controls. the frequency and gmts of lta-positive sera varied with the origin of the patient (chinese greater than north african greater than caucasian, thus paralleling the risk for npc in each ethnic group) and the stage of the disease (stage iv greater than stage i). a positive correlation was found between lta and anti-ebv titres with regard to anti-vca and anti-ebna antibodies. sma were spe ... | 1978 | 215532 |
| epstein-barr virus (ebv) antibody in patients treated by radical radiotherapy for head and neck cancer. | antibody against epstein barr viral capsid (ebv-vca) was measured in 65 patients with primary head and neck cancer referred for radical radiotherapy. sixty-three percent had locally advanced cancer and 37% had early disease. forty-eight percent had detectable antibody in their sera. thirty-three percent had elevated titers (above 1:20 dilution). only 19% of cases with early cancer had increased titers. almost 50% of those with locally advanced cancer or recurrence following treatment had titers ... | 1978 | 215844 |
| [epstein-barr virus and infectious mononucleosis. a clinical study of epstein-barr antibodies (author's transl)]. | thirty-seven patients--aged 2 to 58--with clinical and hematological signs of infectious mononucleosis were studied. thirty patients gave serologic evidence of epstein-barr virus infections; antibodies to viral capsid antigen (vca) appeared very early (only two seroconversions and 4 cases of increased titer were detected); antibodies to early antigen (ea) occured in 25 patients, but only 9 had high titers; antibodies to nuclear antigen (ebna) appeared late in the course of the disease. vca-speci ... | 1978 | 215969 |
| discordance of epstein-barr virus (ebv) specific humoral and cellular immunity in patients with malignant lymphomas: elevated antibody titres and lowered in vitro lymphocyte reactivity. | 1978 | 217561 | |
| biology of the human malignant lymphomas. | 1978 | 216061 | |
| herpesviruses, venereal disease, and cancer. | 1978 | 216293 | |
| histological types of nasopharyngeal carcinoma in an intermediate risk area. | between april and december 1974, 485 cases of npc were seen at the salah azaïz institute in tunis. a histological study was carried out according to a classification that comprises four types of tumour:well-differentiated squamous-cell carcinoma, poorly-differentiated squamous-cell carcinoma, 'nasopharyngeal' type carcinoma and an anaplastic type. some correlation between these histological types and clinical, epidemiological, virological and prognostic features was found. | 1978 | 215511 |
| clinical aspects and natural history of nasopharyngeal carcinoma in western europe. | 1978 | 215512 | |
| descriptive and analytical epidemiology of nasopharyngeal cancer. | information concerning the descriptive and analytical epidemiology of npc that has been reported mainly since the first international symposium on the subject in singapore in 1964 are reviewed. npc is rare in most countries in the world, with an age-adjusted incidence rate of less than 1 per 100,000, and the incidence rate is twice as high in males as in females. chinese of southern origin have a uniquely high risk, the incidence rates per 100,000 being 10--20 in males and 5--10 in females. the ... | 1978 | 215514 |
| histopathological types of nasopharyngeal carcinoma in a low-risk area: japan. | npc in japan was studied histopathologically by examining 816 npcs from among 3 338 biopsies from patients with tumours of the nasopharynx and other parts of the upper respiratory system. in addition, a comparative study on npc among taiwanese and an immunoserological study were carried out. when comparing histological types of malignant tumours of the upper respiratory tract, poorly-differentiated squamous-cell carcinoma predominated only in those of the nasopharynx. malignant nasopharyngeal tu ... | 1978 | 215515 |
| histological typing of nasopharyngeal carcinoma. | there is little agreement about correlations between histological type and the epidemiological and biological characteristics of npc. this is largely due to variations in terminology and diagnostic criteria. the use of the terminology and definitions proposed by who would facilitate and improve international comparisons. | 1978 | 215516 |
| morphological transformation of nasopharyngeal epithelial cells in vitro by epstein-barr virus from b95-8 cells. | tissue fragments of fresh biopsy specimens from the non-neoplastic np mucosa of 20 subjects, from the tumours of 10 npc patients, from the mucosa of freshly removed tonsils from nine subjects and from the primary lesions of seven patients with malignancies of the upper respiratory or alimentary tract other than npc were infected with ebv derived from b95-8 cells. a significantly higher frequency of growth stimulation and a greater mean growth ratio between infected and uninfected fragments from ... | 1978 | 215518 |
| epstein-barr virus-regulation studies on somatic-cell hybrids derived from the fusion of burkitt's lymphoma and nasopharyngeal carcinoma cells with human or mouse partners. | 1978 | 215519 | |
| epstein-barr virus--discovery, properties and relationship to nasopharyngeal carcinoma. | 1978 | 215517 | |
| translocation of a hydrocarbon fluorescent probe between epstein-barr virus and lymphoid cells: an assay for early events in viral infection. | translocation of the hydrocarbon fluorescent probe diphenylhexatriene (dph) between membranes was studied by fluorescence polarization (p) analysis. first, using a model system, the high p value (0.324) of dph-labeled cholesterol/phosphatidylcholine liposomes and the low p value (0.157) of dph-labeled phosphatidylcholine liposomes allowed detection of dph translocation between interacting liposomes. this was monitored by the change in p in either direction. early events during cell-virus interac ... | 1978 | 217012 |
| the interaction of hydrocortisone, interferon and the epstein--barr virus in lymphoid cells. | 1978 | 216555 | |
| the virology and immunology of burkitt's lymphoma in africa and the need for corresponding studies in the high endemic regions of papua new guinea. | 1978 | 220813 | |
| isolation of the epstein-barr virus nuclear antigen from chromatin preparations. | 1978 | 221330 | |
| epstein-barr virus genomes and their biological functions: a review. | 1978 | 221334 | |
| igg and iga antibodies to epstein-barr virus in nasopharyngeal carcinoma patients. | immunoglobulin levels and igg and iga antibodies to epstein-barr virus (ebv), viral capsid antigens (vca) and early antigens (ea) were tested in 67 untreated (5 stage i, 32 stage ii, 16 stage iii and 14 stage iv), 21 treated and 7 recurrent nasopharyngeal carcinoma (npc) patients and 54 normal subjects. the mean serum concentrations of igg, iga and igm in npc patients were higher than those of the normal control. 41.1, 86.3, 94.7 and 95.8% of npc patients had anti-vca titers of greater than or e ... | 1978 | 211015 |
| activation of the alternative complement pathway by human b cell lymphoma lines is associated with epstein-barr virus transformation of the cells. | activation of the alternative complement pathway by human b cell lymphoma lines is correlated with the presence of epstein barr virus (ebv) in the cell genome. ebv-negative b cell lymphoma lines produce little activation of the alternative pathway as measured either by c3 deposition on the cell surface or c3 conversion and consumption of alternative pathway activity in the supernatant serum. by contrast, ebv-positive sublines derived by in vitro ebv conversion of ebv-negative parental lines prod ... | 1978 | 211040 |
| posttransplantation syndrome in children: cytomegalovirus vs epstein-barr virus. | 1978 | 206131 | |
| improved detection of virus-specific igm antibodies. elimination of non-specific igm binding. | a non-specific reaction between human igm and cytoplasmic structures of virus infected cells can often be observed if mgm antibodies to virus antigens are detected by indirect immunofluorescence or by immuno enzyme assays. formaldehyde selectively inactivates the cytoplasmic receptors for human igm without affecting the virus structural proteins. alternatively, receptor-free antigens can be obtained by isolation of nuclei from virus infected cells. due to reduced background, a more specific and ... | 1978 | 211190 |
| studies of epstein-barr virus (ebv)-associated nuclear antigen: solubilization from raji cell chromatin with 5 m-urea-2 m-nacl and fractionation on hydroxyapatite. | ebna-containing chromatin from raji cells was solubilized by treatment with high concentrations of urea and salt and fractionated by hydroxyapatite chromatography. fractions eluting at different phosphate concentrations were analysed for the presence of ebna by means of an anti-ebna-specific 125i-labelled igg absorption assay. the antigen was found in fractions containing non-histone chromatin proteins. | 1978 | 211194 |
| shedding of anti-igm from antibody-coated human lymphoma lines: differences between epstein-barr virus-negative lines and their virus-converted sublines. | in vitro converted, epstein-barr (ebv) genome-carrying cell lines showed a reduced ability to shed surface-bound antibodies, compared to their ebv-free parental b lymphoma lines. on the other hand, antibody degradation was higher with ebv-positive cells. analysis of antibody-coated single cells revealed that cells which failed to redistribute or cap their ligand-bound surface components at 37 degrees c, were also capable of shedding bound antibody. this suggests that capping and shedding are ess ... | 1978 | 207534 |
| heterogeneity of epstein-barr virus. iii. comparison of a transforming and a nontransforming virus by partial denaturation mapping of their dnas. | the dnas of a transforming and a nontransforming epstein-barr virus strain, b95-8 and p3hr-1, were compared by partial denturation mapping. b95-8 viral dna showed a homogeneous denaturation pattern. in contrast, p3hr-1 viral dna was heterogeneous, containing at least two classes of molecules, classified into groups a and b and present in a ratio of about 2:1 to 3:1. no evidence could be obtained that molecules from both groups a and b contain identical sequences present in different orientations ... | 1978 | 211256 |
| isolation of a heterogeneous population of temperature-sensitive mutants of measles virus from persistently infected human lymphoblastoid cell lines. | two human lymphoblastoid b-cell lines, wi-l2 and 8866, were infected with the edmonston strain of measles virus at a multiplicity of infection of 10(-6), and stable persistent infections were established. by immunofluorescence and electron microscopy, the vast majority of cells from both cell lines were expressing viral antigens and releasing virion-like particles. however, very little infectious virus could be detected at 37 degrees c, either by an infectious centers assay or by titration of su ... | 1978 | 210247 |
| demonstration of a surface antigen on epstein-barr virus genome-carrying lymphoid cells: distinction from the virus-determined membrane antigen. | a surface antigen (sa) was detected on epstein-barr virus (ebv)-carrying lymphoid cell lines by indirect membrane immunofluorescence with an antiserum from a rabbit immunized with raji cells; the antiserum had been extensively absorbed with normal human blood and tonsil cells. the sa was not detected on normal human umbilical cord and adult peripheral blood lymphocytes or ebv-negative cell lines. incidences of the sa and ebv-determined membrane antigen (ma) on certain ebv-carrying cell lines wer ... | 1978 | 207776 |
| detection of epstein-barr virus (ebv) dna sequences using in situ hybridization. | in situ hybridization was used to detect epstein-barr virus (ebv) dna sequences under conditions where the virus dna is replicating spontaneously and where it is induced to do so following superinfection. the in situ reaction itself is influenced by several parameters, analogous to conventional nucleic acid hybridization, consideration of which should help to optimize the designing of in situ hybridization reactions in general. both ebv complementary rna (crna) and ebv dna synthesized in vitro c ... | 1978 | 208612 |
| binding to chromosomes of herpes simplex-related antigens in biochemically transformed cells. | association of herpes simplex virus (hsv)-related antigens with chromosomes was demonstrated in human and mouse cells biochemically transformed by hsv that had been irradiated with ultraviolet light. this was accomplished by using peroxidase-anti-peroxidase immunological staining with rabbit antisera that had high neutralizing titers against both hsv-specific thymidine kinase activity and virus infectivity. antisera-against hsv did not react with chromosomes of uninfected cells nor did normal se ... | 1978 | 210457 |
| [incidence and specificity of circulating immune complexes in infectious mononucleosis (proceedings)]. | occurrence of immune complexes in infectious mononucleosis has been investigated by the 125i clq binding assay. increased serum clq-binding activity was found in 87% of the 23 patients studied during the acute stage of the disease. the serum clq-binding material detected has properties identical to those of immune complexes. igg antibodies dissociated from the complexes at acid ph and f (ab)'2 fragments obtained after treatment by pepsin appeared to be directed against the viral capsid antigen o ... | 1978 | 211580 |
| alkaline phosphatase in epstein-barr viral nuclear antigen--positive cell lines. | the production and nature of alkaline phosphatase were studied in epstein-barr viral nuclear antigen-positive, surface membrane immunoglobulin negative-cell lines established from two patients, one with acute myeloid leukemia and one with acute lymphoblastic leukemia. the acute myeloid leukemia-derived cells contained myeloid alkaline phosphatase, while the acute lymphoblastic leukemia-derived cells contained lymphoid alkaline phosphatase. the presence of the myeloid-specific enzyme in a surface ... | 1978 | 211588 |
| human malignant lymphomas in vitro. characterization of biopsy cells and establishment of permanent cell lines. | a series of 55 biopsies from different types of malignant lymphomas were characterized in short-term culture experiments and during prolonged growth in vitro. the majority of the lymphocytic lymphomas and half of the histiocytic lymphomas expressed surface immunoglobulin, either in monoclonal or polyclonal form, indicating b-lymphocyte derivation. no lysozyme production was noted in either type of lymphoma, giving further support to the notion that histiocytic lymphomas are not truly histiocytic ... | 1978 | 211794 |
| infection with epstein-barr virus. | 1978 | 212156 | |
| the values of epstein-barr virus titers as a diagnostic shortcut in infectious mononucleosis: two case reports. | 1978 | 212244 | |
| epstein-barr virus. is it timely to discuss a vaccine? | 1978 | 208671 | |
| the epstein-barr virus (ebv) in human pathology. ii. serologic profiles of ebv infections. | antibodies to ebv induced intracellular antigens, (vca, ea, na) and vca-igm antibodies have been investigated to define ebv serologic profile of 245 individuals. this profile was a first studied in ebv primary infections. in infectious mononucleosis, the efficiency of ebv serodiagnosis is lower than paul bunnel davidsohn reaction (pbd) : primary infection profile is only characterised in 80% of the positive pbd infectious mononucleosis (im). on the other hand, ebv antibodies are preponderant for ... | 1978 | 208675 |
| expression of altered ribonucleotide reductase activity associated with the replication of the epstein-barr virus. | 1978 | 210572 | |
| current assessment of "case clustering" of lymphomas and leukemias. | 1978 | 210923 | |
| genetics of neoplasia--impact of ecogenetics on oncogenesis. a review. | 1978 | 207190 | |
| long-term cultivation of plasma cell leukemia cells and autologous lymphoblasts (lcl) in vitro: a comparative study. | two long-term cell lines were established in vitro from the peripheral blood of a patient with plasma cell leukemia: one line with plasma cell proliferation, the other with lymphoblastoid cell proliferation (lcl). the 9-month-old plasma cell line showed the typical morphology of plasmoblasts. the cells neither had b- nor t-lymphocyte characterisitics, were ebv negative, and showed aneuploidy with various marker chromosomes, including the 14 q+ marker. the cytogenetic findings indicate a monoclon ... | 1978 | 207377 |
| a new tumour-derived transforming strain of epstein-barr virus. | 1978 | 205793 | |
| epstein-barr virus is not synonymous with infectious mononucleosis. | 1978 | 205830 | |
| reversible inhibition by phosphonoacetic acid of human b lymphocyte transformation by epstein-barr virus. | 1978 | 208257 | |
| mechanisms of infection with epstein-barr virus. iii. the synthesis of proteins in superinfected raji cells. | 1978 | 208266 | |
| autoradiographic detection of epstein-barr virus (ebv)-associated early antigen in a variety of ebv dna-containing lymphoblastoid cell lines previously designated as nonproducers. | by using autoradiography combined with 125i-labeled igg prepared from human sera containing high anti-ea (early antigen) antibody titers, ea has been detected in a low fraction of cells in a variety of ebv dna-containing cell lines previously designated as negative for this antigen. these positive cell lines are highly inducible for ea following treatment with iododeoxyuridine (iudr) and also contain multiple copies of the virus genome on average. the correlation between iudr inducibility and sp ... | 1978 | 204604 |
| epstein-barr virus-determined nuclear antigen in malignant epithelial cells of nasopharyngeal carcinoma. | tumor tissue from lymph node metastasis of nasopharyngeal carcinoma (npc) was successfully heterotransplanted into an athymic nude mouse and the tumor grown in the nude mouse was identical in its morphology to that form the patient by optical and electron microscopy. tumor cells at passage 2 were dispersed in vitro by enzymic digestion and smeared. epstein-barr virus-determined nuclear antigen (ebna) was demonstrated in malignant epithelial cells of the smear. | 1978 | 205477 |