PMID(sorted ascending)
epstein-barr virus dna in reed-sternberg cells of hodgkin's disease is frequently associated with cr2 (ebv receptor) expression.we studied 44 cases of hodgkin's disease for the presence of epstein-barr virus (ebv) dna, its localization and the expression of the ebv receptor on the tumour cells. ebv dna was found in 52% (16/31) of the hodgkin's lymphomas using the polymerase chain reaction. with a very sensitive non-radioactive dna in situ hybridization technique in combination with immunohistochemistry for cd 30 or cd 15 antigens, ebv dna was localized to reed-sternberg cells and its mononuclear variants. the relationshi ...19921321787
the expression of the ebv latent membrane protein (lmp-1) is independent of cd23 and bcl-2 in reed-sternberg cells in hodgkin's disease.a series of 33 cases of hodgkin's disease was investigated for the presence of the ebv encoded latent gene product lmp-1 and of cd23 using immunohistochemical techniques. the expression of bcl-2 was examined in a subset of cases. lmp-1 was detected in the reed-sternberg cells in 15 cases. although lmp-1 is known to upregulate cd23 and bcl-2, there was no correlation between the expression of lmp-1 and the detection of cd23 and bcl-2 in reed-sternberg cells.19921321789
requirements for the production of high-titre c3 nephritic factor (nef) antibody in vitro.c3 nephritic factor (nef) is an igg autoantibody directed against neoantigenic determinants of the alternative c3 convertase ( structural and functional studies require important amounts of this antibody, which are difficult to obtain from patients' sera. we have developed a method for increasing nef production in vitro. epstein-barr virus is a herpes virus which transforms b lymphocytes. some authors were able to induce the production of nef in vitro after infection with epstein-barr vi ...19921321794
sample age and epstein-barr virus transformation of cryopreserved lymphocytes. 19921321805
ig mu-epsilon isotype switch in il-4-treated human b lymphoblastoid cells. evidence for a sequential switch.ige is produced by b lymphocytes that have undergone a deletional rearrangement of their ig h chain gene locus, a rearrangement that joins the switch region of the mu gene, s mu, with the corresponding region of the epsilon gene, s epsilon. to examine the resulting composite s mu-s epsilon junctions of human lymphoid cells, we have used a polymerase chain reaction strategy to clone the switch regions of the human myeloma u266 and of two ige-producing human cell lines generated by treatment of ly ...19921321850
detection of human herpesvirus 6 in tissues involved by sinus histiocytosis with massive lymphadenopathy (rosai-dorfman disease).after preliminary serologic data demonstrated elevated antibody titers to human herpesvirus (hhv) 6 in patients with sinus histiocytosis with massive lymphadenopathy (shml) or rosai-dorfman disease, tissues were examined from 9 patients with classical shml to search for evidence of hhv-6 infection. involved tissues from 7 of the 9 patients had detectable hhv-6 by in situ hybridization: tissue from 1 had detectable epstein-barr virus genome but no hhv-6 and tissue from another had no detectable h ...19921321861
[detection of epstein-barr virus dna in salivary gland tumors].to evaluate the relations between salivary gland tumors and epstein-barr virus (ebv), the levels of ebv-related antibodies were examined, and detection of ebv nuclear antigen (ebna) and ebvdna in tumor tissue was attempted by the anti-complement immunofluorescence technique and polymerase chain reaction, respectively. the mean vca-igg antibody level was increased to 925 (80-2560) in warthin's tumor, 496 (40-2560) in mucoepidermoid tumor, and 206 (40-640) in pleomorphic adenoma. the positive rate ...19921321900
a pathological and immunohistological case report of fatal infectious mononucleosis, epstein-barr virus infection, demonstrated by in situ and southern blot hybridization.we present an autopsy case of 20-month-old boy who had a fulminant course of infectious mononucleosis, with severe hepatic failure. autopsy revealed marked infiltration of immunoblasts in the lymph nodes, liver, spleen, thymus and kidneys. we identified a large number of epstein-barr virus (ebv) genomes in the immunoblasts of the lymph nodes, liver and spleen by in situ hybridization. ebv genomes were also detected in the liver and spleen by southern blot hybridization. histology of the liver re ...19921321997
morphological findings on peripheral nerve biopsies in 15 patients with human immunodeficiency virus infection.a peripheral nerve biopsy was performed in 15 patients with human immunodeficiency virus (hiv) infection and polyneuropathy. two cases [1 asymptomatic, 1 aids-related complex (arc)] presented with chronic inflammatory demyelinating polyneuropathy; there was 1 case (asymptomatic) of mononeuropathy multiplex and 12 cases (1 asymptomatic, 1 arc, 10 aids) with distal symmetrical polyneuropathy. epi- or endoneurial microvasculitis was observed in 6 cases. electron microscopy showed that nerve fiber l ...19921322001
correlation of pretransplant viral serology and complications of bone marrow transplantation.latent herpes viruses such as herpes simplex virus, cytomegalovirus (cmv), and varicella zoster virus are often reactivated after bone marrow transplantation, giving rise to infections. in contrast, epstein-barr virus infections rarely occur. significant mortality is induced especially by pneumonitis, most often caused by cmv. immunosuppression and pancytopenia caused by cmv increase the risk of bacterial infections and invasive fungal infections. herpes viruses may increase the risk of acute an ...19921322186
epstein-barr virus infection in allogeneic marrow grafting: lessons for transplant physicians and virologists.the relationship between epstein-barr virus (ebv) and the host is profoundly disturbed by allogeneic bone marrow transplantation (bmt) because ebv resides in the recipient's hematopoietic system, which has to be destroyed in the majority of cases, and in the donor's hematopoietic system, i.e., the marrow graft. we have shown that ebv may be eradicated from some bmt recipients and that the virus may be transferred with the marrow graft. during the immediate post-transplant period oropharyngeal eb ...19921322190
interferon-gamma gene expression in human b-cell lines: induction by interleukin-2, protein kinase c activators, and possible effect of hypomethylation on gene regulation.human interferon-gamma (ifn-gamma) is an important immunomodulatory protein produced predominantly by t cells and large granular lymphocytes (lgls). whereas large amounts of data have been accumulated regarding ifn gamma gene expression in these two cell types, little information about ifn gamma expression in other cell types exists. in this study, we have analyzed the production of ifn gamma by the epstein-barr virus (ebv)-positive b-cell line, jlp(c), derived from a patient with burkitt's lymp ...19921322203
ultraviolet mutagenesis in human lymphocytes: the effect of cellular transformation.we have assessed the role of cellular transformation in ultraviolet (uv)-induced mutagenic events in human cells. to maintain uniformity of genetic background and to eliminate the effect of dna repair, primary nontransformed lymphocytes (t-cells) and epstein-barr virus-transformed lymphocytes (b-cells) from one patient (xp12be) with the dna repair-deficient disorder xeroderma pigmentosum (group a) were transfected with the mutagenesis shuttle vector pz189. parallel control experiments were perfo ...19921322318
expression of the epstein-barr virus (ebv)-encoded membrane protein lmp1 impairs the in vitro growth, clonability and tumorigenicity of an ebv-negative burkitt lymphoma a previous study on several independently established epstein-barr virus (ebv)-converted sublines of the ebv-negative burkitt lymphoma (bl) line bl41, we found that expression of the virally encoded membrane protein lmp1 was accompanied by reduced agarose clonability and tumorigenicity. in order to investigate whether lmp1 can induce these phenotypic changes by itself, we have now studied the growth in suspension culture, the clonability in agarose and the tumorigenicity in immunosuppressed a ...19921322379
human b-cell lymphoma in severe combined immunodeficient mice after active infection with epstein-barr virus.b-cell lymphomas (bcl) occur with increased frequency in immunosuppressed patients. bcl develop in severe combined immunodeficient (scid) mice after engraftment with human peripheral blood leukocytes (pbl; hu-pbl-scid mice) and infection with epstein-barr virus (ebv). the contributions of latent and active ebv infection to bcl development, the potential enhancing effects of immunosuppressive therapy, and inhibitory effects of antiviral therapy on the development of bcl in this model were studied ...19921322566
selective inhibition of the 3'-to-5' exonuclease activity associated with epstein-barr virus dna polymerase by ribonucleoside 5'-monophosphates.epstein-barr virus (ebv) dna polymerase possesses a proofreading 3'-to-5' exonuclease activity (tsurumi, t. (1991) virology 182, 376-381). the 3'-to-5' exonuclease activity can be selectively inhibited by ribonucleoside 5'-monophosphates, while no inhibition of the dna polymerase activity can be observed even when the template/primer concentrations are rate-limiting. deoxynucleoside monophosphates except 5'dgmp have almost no effect on the exonuclease activity. of the four ribonucleoside monopho ...19921322605
screening for epstein-barr and human cytomegalovirus in normal and abnormal cervical smears by fluorescent in situ cytohybridization.using the routine papanicolaou test for grouping, 35 normal and 148 abnormal cervical smears were screened by fluorescent in situ cytohybridization for epstein-barr (ebv) and human cytomegalovirus (hcmv). viral presence was detected in 55% of all hybridized smears while routine papanicolaou tests exposed no morphological evidence for hcmv or ebv. thus in situ cytohybridization is necessary and suitable for direct identification of ebv and hcmv. the prevalence of viral infection for women with no ...19921322652
a rheumatoid factor from a normal individual encoded by vh2 and v kappa ii gene gain insight into the immunoglobulin variable-region repertoire of anti-igg antibodies (rheumatoid factors [rf]), we characterized the vh and v kappa gene segments utilized in an igm-rf-secreting lymphoblastoid cell line (ssh23) isolated from a normal individual.19921322670
lethal midline granuloma (peripheral t-cell lymphoma) after lymphomatoid papulosis.a japanese woman with an 8-year history of lymphomatoid papulosis (lp) had lethal midline granuloma (lmg) develop at the age of 51 years. there were histologic similarities between lp and lmg seen in this patient. surface phenotypic studies on nasal and cutaneous lesions demonstrated a population of t-cells expressing cd2, cd4, cd25, cd30, and histocompatibility antigen-dr (hla-dr). genotypic analyses of nasal and skin biopsy specimens disclosed a clonal rearrangement of the beta t-cell receptor ...19921322784
distribution of type a and type b ebv in normal individuals and patients with head and neck carcinomas in taiwan.the subtypes of epstein-barr virus (ebv) according to the ebna 2 gene were investigated in taiwan by the polymerase chain reaction (pcr) and by southern blot hybridization. the materials included 53 nasopharyngeal carcinoma (npc) biopsies, 49 other head and neck cancers and 32 throat washings of normal individuals. ebv dna was found in all npc biopsies, 27 of 49 other head and neck carcinomas and 81% of normal individuals. type a ebv was the predominant type of ebv in both normal individuals and ...19921322927
a novel herpes simplex virus gene (ul49a) encodes a putative membrane protein with counterparts in other herpesviruses.comparative analysis of dna sequences located between the coding regions of genes ul49 and ul50 of herpes simplex virus types 1 and 2 (hsv-1 and -2) has revealed a small open reading frame (orf) of 91 and 87 codons respectively with the characteristics of a genuine protein-coding region. the predicted protein products are clearly related and exhibit features of membrane-inserted proteins, with potential n-proximal signal peptides and c-proximal membrane anchor regions. counterparts are present i ...19921322965
epstein-barr virus: does it cause cancer? 19921322997
[infections after bone marrow transplantation in childhood].in 66 children having undergone bone marrow transplantation (bmt) the occurrence of infections was studied retrospectively. bacterial infections were mostly found in the early period after transplantation before marrow engraftment. the analysis of positive blood cultures showed a dominance of gram-positive bacteria, especially of coagulase-negative staphylococci. cytomegalovirus (cmv) infections were most important, because of its high rate and the risk of cmv associated interstitial pneumonia ( ...19921323001
nuclear localization of the epstein-barr virus/c3d receptor (cr2) in the human burkitt b lymphoma cell, raji.epstein-barr virus/c3d receptor (cr2) is a glycoprotein of mol. wt 140,000 expressed on the surface of raji cells. we previously isolated phosphorylated cr2 from purified raji cell nuclei. we have analyzed the nuclear localization of cr2 by electron microscope immunochemistry of thin sections of raji cells and we have compared the binding properties of cr2 expressed on purified plasma membranes or nuclei. anti-cr2 mab immunogold labeling of thin sections of raji cells identified cr2 at the nucle ...19921323059
in situ hybridization for epstein-barr virus noti repeats in posttransplant lymphoproliferative disorder.epstein-barr virus-associated posttransplant lymphoproliferative disorder (ptld) is a serious complication of solid organ transplantation. in ptld, b-cell expansions range from reactive hyperplasias to large cell lymphomas and are often associated with active epstein-barr virus (ebv) infection. the lymphoproliferations may infiltrate transplant allografts and therefore may need to be distinguished from acute cellular rejection (acr). a total of 36 tissue specimens from 11 transplant patients (si ...19921323104
multiplex polymerase chain reaction.the polymerase chain reaction (pcr) is a widely utilized assay for specifically amplifying small fragments of dna. multiplex pcr is the amplification of more than one dna fragment per reaction and has many potential uses. when more than one primer set per reaction tube is utilized, the total number of tubes in any one experiment may be reduced, conserving expensive reagents and decreasing possible contamination. multiplex pcr allows for an assay of the gene of interest and assures that the ampli ...19921323106
in situ localization of epstein-barr virus in thymic carcinoma.epstein-barr virus (ebv) genome is associated with a variety of lymphoid and epithelial malignancies. ebv dna has been detected in some cases of thymic carcinoma, but the cellular locus of the virus has never been defined. detection of ebv has also been reported in normal thymus, thymic lymphoid hyperplasia, and thymoma by some investigators but not by others. in order to better define the association of the virus with benign and malignant thymic tissues and to characterize its cellular locus, w ...19921323108
pathogenesis of sjögren's syndrome.sjögren's syndrome is a systemic autoimmune disease characterized by lymphocytic infiltrations of lacrimal and salivary glands. ss patients produce a variety of autoantibodies, including rf and ana. genetic factors, including hla-dr3, predispose to primary ss. in contrast to normal sgs, the ss sg epithelial cells express high levels of hla-dr antigens. this class ii gene expression on the target organ may represent the structural basis for hla-associated disease susceptibility. the glands are in ...19921323135
treatment of central nervous system b lymphoproliferative syndrome by local infusion of a b cell-specific monoclonal antibody.a 9-month-old infant developed epstein-barr virus-induced lymphoproliferative syndrome with mediastinal and central nervous system localizations, associated with mediastinal tuberculosis, 5 months after heart transplantation. as a combination of anti-b cell antibodies (cd21- and cd24-specific) and recombinant interferon alpha 2b, given intravenously, was not effective on the central nervous system disease, the anti-cd21 antibody was infused intrathecally via an ommaya reservoir. high local conce ...19921323149
a recent decrease in the time to development of monomorphous and polymorphous posttransplant lymphoproliferative disorder.we have noted a decrease in the time to development of posttransplant lymphoproliferative disorder (ptld) over the last two and one-half years in our multiorgan transplant program. from february 1965 until december 1990, 1622 transplants were performed including 1489 kidneys (ktxp), 87 livers (ltxp), and 46 pancreata. between february 1965 and july 1988 (group 1), there were 1260 transplants performed and nine cases of either monomorphous ptld (m-ptld, n = 8) or polymorphous ptld (p-ptld, n = 1) ...19921323150
malignancy in renal transplant recipients. 19921323158
epstein-barr and human immunodeficiency viruses in acquired immunodeficiency syndrome-related primary central nervous system lymphoma.the prevalence of epstein-barr virus (ebv) and human immunodeficiency virus (hiv) in acquired immunodeficiency syndrome (aids)-related primary central nervous system (cns) lymphoma was examined. deoxyribonucleic acid (dna) extracted from 12 formalin-fixed, paraffin-embedded tumors was used as substrate for the polymerase chain reaction (pcr). targets for amplification were the ebna-1 region of ebv, the gag region of hiv, and a single copy cellular sequence as a control. the cases studied were au ...19921323221
detection of epstein-barr virus genome in ki-1 (cd30)-positive, large-cell anaplastic lymphomas using the polymerase chain (cd30)-positive, large-cell anaplastic lymphoma (lcal) is a distinctive subset of non-hodgkin's lymphoma; morphologically, the neoplastic cells of lcal may closely resemble reed-sternberg cell variants of hodgkin's disease. the neoplastic cells in hodgkin's disease are often cd30-positive, as are some of the transformed lymphocytes in infectious mononucleosis. recent evidence suggests an etiologic role for the epstein-barr virus (ebv) in hodgkin's disease. because of the phenotypic similari ...19921323222
recurrent cutaneous anaplastic large cell (cd30+) lymphoma associated with epstein-barr virus. a case report with 9-year follow-up.cd30-positive large cell cutaneous t-cell lymphomas are known to be associated with the human t-cell leukemia/lymphoma virus type i. we present a case of anaplastic large cell lymphoma that recurred three times during 9 years at different sites. molecular studies [polymerase chain reaction (pcr), in situ hybridization] showed epstein-barr virus (ebv) genome in biopsy samples of this first reported case of ebv-associated cutaneous anaplastic large cell lymphoma. this case was human t-lymphotropic ...19921323224
clinical, epidemiologic, and virologic studies in four clusters of the chronic fatigue syndrome.the purpose of this study is to provide a case definition of chronic fatigue syndrome in an outbreak occurring in the nevada-california region to evaluate candidate etiologic agents and observe the natural history of the illness.19921323246
epstein-barr virus infection-associated renal disease: diagnostic use of molecular hybridization technology in patients with negative serology.there are only a few reports of renal disease associated with epstein-barr virus (ebv) infection. the diagnosis of ebv infection in these previously reported patients was based primarily on positive serology. two patients with renal disease who, despite repeatedly negative serologies, were shown by molecular hybridization techniques--in situ hybridization (ish) and polymerase chain reaction (pcr)--to have ebv infection are reported here. site-specific molecular probes directed against specific, ...19921323338
detection of distinct epstein-barr virus genotypes in npc biopsies from southern chinese and caucasians.using the polymerase chain reaction (pcr) to analyze paraffin sections from 12 caucasian patients, we detected only the prototype f epstein-barr virus (ebv) in 10 specimens from patients with nasopharyngeal carcinoma (npc). this is in contrast to the higher frequency of association of "f" variants in npc biopsies from southern chinese. the results of ebv genotyping support evidence that the ebv strains associated with npc in the southern chinese population differ from those found in caucasians. ...19921323539
the epstein-barr virus r transactivator (rta) contains a complex, potent activation domain with properties different from those of vp16.rta, encoded by epstein-barr virus (ebv), is a potent activator of transcription via enhancer sequences located upstream of several viral genes. to identify the domains of rta that facilitate transcription by interacting with cellular transcription factors, different segments of rta were linked to the dna binding domain of yeast transactivator gal4 (residues 1 to 147). these gal4-rta fusion proteins were tested in transfected cells for their ability to activate the adeno e1b promoter with an ups ...19921323708
partial elimination of epstein-barr virus plasmids from burkitt's lymphoma cells by transfecting the bzlf1 gene.epstein-barr virus (ebv) nonproducer raji cells stably maintain approximately 45 copies of the ebv genome per cell, depending on the presence of the ebv-determined nuclear antigen 1 (ebna-1) protein. we found that transfection of the ebv bzlf1 gene causes the disappearance of ebna proteins on western blots (immunoblots). on the basis of these results, we attempted to eliminate ebv plasmids in raji cells by transfecting a bzlf1 plasmid. among 33 clones that were cotransfected with a bzlf1 plasmid ...19921323712
characterization of the deletion and rearrangement in the bamhi c region of the x50-7 epstein-barr virus genome, a mutant viral strain which exhibits constitutive bamhi w promoter activity.epstein-barr virus infection of peripheral b lymphocytes predominantly results in a latent infection, with a concomitant growth transformation of the infected cells. these cells express six nuclear antigens (ebnas) and three membrane antigens. transcription of all the ebna genes is driven by one of two promoters, cp or wp, located near the left end of the viral genome, and the activities of these promoters are mutually exclusive. we have previously shown that wp is exclusively used during the in ...19921323716
epstein-barr virus dna recombines via latent origin of replication with the human genome in the lymphoblastoid cell line rgn1.we show here that in a lymphoblastoid cell line epstein-barr virus dna recombines with the human genome. the genetic exchange involves the orip region of the virus. a junction between viral and human dna from this line has been cloned and sequenced. the results indicate that the integration of epstein-barr virus dna involves a region of the human genome which contains internal short repetition. an 800-bp probe has been isolated from the human part of the junction. this probe has been used to sho ...19921323719
[a case of acute hepatitis induced by epstein-barr virus complicated with disseminated intravascular coagulation]. 19921323724
marked variation in the size of genomic plasmids among members of a family of related epstein-barr viruses.epstein-barr virus (ebv) genomes in the p3j-hr-1 (hr-1) burkitt lymphoma cell line rearrange at a high rate. previously described deletions and rearrangements in hr-1 cells have been found at sites of ebv replication in vivo, suggesting that dna rearrangement may be an integral aspect of ebv biology and pathogenesis. we examined the structure of linear ebv genomes in subcultures of hr-1 cells using contour-clamped homogenous electric field gel electrophoresis. we developed a second pulsed electr ...19921323852
ebv's open sesame. 19921323889
epstein-barr virus vaccines.epstein-barr virus (ebv) is a human viral pathogen of considerable importance. more than 95% of the human population world-wide becomes infected with the virus during childhood, although in the west infection may be delayed until adolescence. the infection only has an undesirable significant clinical outcome in a tiny minority of cases, but because the virus is so ubiquitous the minority is numerically very significant. the virus is associated with two important human cancers, endemic burkitt's ...19921323899
are endogenous retroviruses involved in human autoimmune disease?a role for viruses in the etiopathogenesis of human autoimmune diseases has long been suspected but has not yet been proven. in sjögren's syndrome (ss), there is continuing experimental support for the possible involvement of epstein-barr virus. since the advent of aids, there is also great interest in retroviruses and autoimmune disease. we previously reported that 30% of ss patients and 36% of systemic lupus erythematosus (sle) patients have serum antibodies to the p24 gag protein of hiv-1. we ...19921323968
interleukin-4 stimulates immunoglobulin secretion by epstein-barr virus (ebv)-activated tonsillar b cells, and by ebv-transformed lymphoblastoid b cell lines without increasing cell division.freshly prepared epstein-barr virus-transformed b lymphoblastoid cell lines derived from five different donors were tested for their responses to recombinant human interleukin-4 and to low molecular weight b cell growth factor. in the absence of either cytokine, all five lines secreted immunoglobulin of more than one isotype (igm, igg, and iga, but not ige). stimulation with interleukin-4 resulted in a significant increase in immunoglobulin secretion, but did not enhance cell division measured b ...19921324027
direct detection of epstein-barr virus dna from a single reed-sternberg cell of hodgkin's disease by polymerase chain reaction.eleven cases of hodgkin's disease (hd) were examined for the presence of the epstein-barr virus (ebv) genome, using the polymerase chain reaction (pcr) to detect ebv dna in whole paraffin-embedded tissue specimens and in single cells picked out from the specimens with a micromanipulator. the ebv genome was detected in 5 of the 11 cases by conventional pcr. single cell pcr demonstrated the ebv genome in reed-sternberg cells from all the ebv-positive cases, but not from any of the ebv-negative cas ...19921324234
[benign lymphoepithelial cysts of the parotid in a patient with hiv infection].we report on a hiv-positive patient presenting bilateral enlargement of the parotid gland. the ultrasound examination demonstrated multiple lesions with cystic aspect. a partial surgical excision of the parotid gland was performed, and histologic examination confirmed the diagnosis of a cystic benign lymphoepithelial lesion. the specimens which were tested for epstein-barr virus and cytomegalovirus by in situ hybridization proved negative. the clinical and pathologic features of this unusual sal ...19921324394
hemiplegia--a rare complication of acute epstein-barr virus (ebv) infection.a 32-year-old male presented to hospital with a transient hemiplegia associated with a rash and systemic upset. he was found to have an acute epstein-barr virus (ebv) infection. hemiplegia complicating glandular fever has been described but once previously.19921324523
chronic verrucous varicella-zoster virus infection in patients with the acquired immunodeficiency syndrome (aids). histologic and molecular biologic findings.verrucous skin lesions have been attributed to various herpes viruses in immunosuppressed patients, including those with human immunodeficiency virus infection (hiv). we examined such lesions from six hiv-infected patients to determine the range of microscopic findings present and to establish which herpesviruses were present. verrucous epidermal hyperplasia, pseudocarcinomatous hyperplasia, and massive hyperkeratosis correlate with the warty clinical appearance of the lesions. herpetic cytopath ...19921324620
detection of epstein-barr virus in the brain by the polymerase chain reaction.epstein-barr virus (ebv) has been implicated in a variety of central nervous system syndromes. in a few well-studied patients, ebv has been detected by viral isolation or ebv dna has been found by southern hybridization analysis. using polymerase chain reaction, we evaluated brain biopsy specimens from 24 patients for the presence of ebv genomes. ebv dna was found in brain specimens from 18 patients in whom presence of the virus in the brain was suspected clinically or on the basis of serologica ...19921324632
phosphorylation of the epstein-barr virus nuclear antigen 2.a major in vivo phosphorylation site of the epstein-barr virus nuclear antigen 2 (ebna-2) was found to be localized at the c-terminus of the protein. in vitro phosphorylation studies using casein kinase 1 (ck-1) and casein kinase 2 (ck-2) revealed that ebna-2 is a substrate for ck-2, but not for ck-1. the ck-2 specific phosphorylation site was localized in the 140 c-terminal amino acids using a recombinant trpe-c-terminal fusion protein. in a similar experiment, the 58 n-terminal amino acids exp ...19921324672
epstein-barr virus dna detection in gingival tissues of patients undergoing surgical extractions.the main oral manifestation of epstein-barr virus (ebv) infection is hairy leukoplakia, a lesion associated with the acquired immunodeficiency syndrome (aids) and occasionally in other immunocompromised patients. however, the recent literature describes the presence of viral genome in clinically normal oral tissues. the purpose of this work was to investigate these occult ebv infections in gingival epithelium. the southern blot method with 32p-radiolabelled dna probes under stringent conditions ...19921324707
inhibitory effects of dihydroagarofuran sesquiterpenes on epstein-barr virus search for possible antitumor promoters, we carried out a primary screening of thirty-seven dihydroagarofuran sesquiterpenes from tripterygium wilfordii hook fil. var. regelii makino and euonymus sieboldianus blume, using their possible inhibitory effects on the epstein-barr virus early antigen (ebv-ea) activation which is induced by 12-o-tetradecanoylphorbol-13-acetate (tpa) in raji cells. some of these sesquiterpenes, triptofordin f-2 (takaishi et al., 1988), 1,2,6,8,15-pentaacetoxy-9-benzo ...19921324784
cytogenetic characterization of b-cell lymphomas from severe combined immunodeficiency disease mice given injections of lymphocytes from epstein-barr virus-positive donors.we analyzed the karyotype of 27 b-cell lymphomas of human origin that developed in mice with severe combined immunodeficiency disease following the injection of peripheral blood leukocytes from epstein-barr virus-seropositive donors. three tumors had clonal abnormalities detected with conventional techniques, 2 had trisomy 11, and 1 had a del(6)(q21q25). one other tumor had trisomy 11 detected with fluorescence in situ hybridization. twelve tumors had a normal karyotype, 11 tumors had nonclonal ...19921324790
effects of il-4, il-5, and il-6 on growth and immunoglobulin production of epstein-barr virus-infected human b the present study we investigated whether interleukin-4 (il-4), il-5, and il-6 could enhance the efficiency of epstein-barr virus (ebv) transformation for the generation of specific human monoclonal antibody (humab)-producing b-cell lines directed against erythrocyte rhesus(d) antigen. in newly ebv-infected b cells, il-4 and il-6 caused a comparable enhancement of proliferation and of total igg and iga production. il-6 showed a much stronger effect than il-4 on igm production, whereas il-4 wa ...19921324802
induced cd25 expression in a human b-lymphoma cell line transfected with the epstein-barr virus nuclear antigen 2 gene.two ebv-negative human b-lymphoma cell lines, bjab and dg75, were transfected with an epstein-barr virus (ebv) nuclear antigen 2 (ebna-2) gene, which plays a critical role in the ebv-induced immortalization of primary b lymphocytes. furthermore, dg75 cells were co-transfected with the ebna-2 gene and a latent membrane protein (lmp) gene. expression of eight surface antigens on the resultant ebna-2-expressing cell clones was analyzed by flowcytometry. none of the ebna-2-expressing cell clones der ...19921325025
clinical observation and neurological outcomes in "alice in wonderland" syndrome.ten patients, aged from 3 to 10 years, who had either had attacks of perception errors of body schema and objects or had visual hallucination were enrolled from 1987 to 1990. seven were boys and three, girls. the metamorphopsia or visual hallucination that has been named the "alice in wonderland syndrome" (aiws) were the leading presentations. all the patients had preceding episodes of upper respiratory tract infection (uri) from two days to four weeks previously except for one who had had chick ...19921325095
molecular pathogenesis of hiv-associated lymphomas.the data presented here indicate that the pathogenesis of aids-nhl is variably associated with multiple genetic alterations including monoclonal ebv infection, oncogene activation (c-myc, n-, ki-ras) and tumor suppressor gene (p53) inactivation. up to three (3 cases) or four (1 case) different lesions have been observed in the same tumor. the distribution of these lesions among the various histotypes is heterogeneous, although some preferential associations have been found either between lesion ...19921325169
ebv-induced human b cell lymphomas in hu-pbl-scid mice.epstein-barr virus (ebv) infection is associated with burkitt's lymphoma (bl) in normal individuals and immunoblastic b cell lymphomas in immunosuppressed or hiv-infected individuals. scid mice reconstituted with human peripheral blood leukocytes (hu-pbl-scid) from ebv-seropositive donors also may develop spontaneous b cell lymphomas which histologically and phenotypically resemble post-transplant tumors, and are distinct from bl. these tumors always contain ebv dna. we have noted three differen ...19921325170
association of epstein-barr virus with primary central nervous system lymphoma in aids. 19921325171
long-term results of pediatric heart transplantation. 19921325185
restoration of superoxide generation to a chronic granulomatous disease-derived b-cell line by retrovirus mediated gene transfer.failure of a superoxide generating system, the nadph oxidase, present in neutrophils and other phagocytes gives rise to chronic granulomatous disease (cgd), a group of single-gene inherited disorders all characterized by an extreme susceptibility to pyogenic infection, with potentially fatal consequences. about 30% of cgd cases are caused by an autosomally inherited deficiency of a 47-kd cytoplasmic component of the oxidase (p47-phox). epstein-barr virus (ebv) immortalized b-lymphocyte lines est ...19921325210
human b-cell interleukin-10: b-cell lines derived from patients with acquired immunodeficiency syndrome and burkitt's lymphoma constitutively secrete large quantities of interleukin-10.a recent addition to the lymphokine network is human il-10 (hil-10). this novel lymphokine has striking homology to bcrf1 protein, the product of a previously uncharacterized open-reading frame in the epstein-barr virus (ebv) genome. to date, il-10 expression has been described in several t clones induced with anti-cd3 and phorbol myristate acetate (pma), in monocytes stimulated with lipopolysaccharide (lps), and in murine b-cell lymphomas. we sought to determine whether human b cells express hi ...19921325212
epstein-barr virus in acquired immune deficiency syndrome (aids) and non-aids primary central nervous system lymphoma.primary central nervous system lymphoma (pcnsl) still occurs mainly in patients who are immunosuppressed, but its incidence is rising dramatically among immunocompetent individuals. the epstein-barr virus (ebv) has been detected by in situ hybridization in pcnsl tumor tissue from patients who are immunodeficient, but not from patients who are immunocompetent. using the more sensitive polymerase chain reaction (pcr) technique, the authors analyzed pcnsl tissue from 13 patients with acquired immun ...19921325277
transition of phenotypic dimorphism with regard to spontaneous sister chromatid exchange in epstein-barr virus-transformed bloom's syndrome lymphoblastoid cell lines.we recently established four lymphoblastoid cell lines (lcls) by infecting the peripheral blood of four japanese patients suffering from bloom's syndrome (bs) with epstein-barr virus (ebv). during the course of propagating these cell lines, two of them exhibited dimorphism regarding spontaneous sister chromatid exchange (sce), i.e., a mixed population consisted of cells with extremely high sce levels characteristic of bs and cells with low sce levels indistinguishable from that of normal control ...19921325430
primary cerebral lymphoma: a study of 47 cases probed for epstein-barr virus determine the prevalence of epstein-barr virus genome in primary cerebral lymphomas occurring in the absence of immune suppression.19921325479
epstein barr virus (ebv) encoded small rnas: targets for detection by in situ hybridisation with oligonucleotide develop a rapid, sensitive, and specific non-isotopic in situ hybridisation (nish) procedure for the detection of epstein-barr virus in formalin fixed, paraffin wax embedded tissues.19921325480
criteria for the definition of epstein-barr virus association in hodgkin's disease.there is a clear association between the epstein-barr virus (ebv) and hodgkin's disease (hd). ebv is not, however, detectable within the affected tissues of all cases. the proportion of positive cases varies from 15-79% depending on the assay used to detect ebv. the techniques utilised vary not only in sensitivity but in their ability to detect viral dna, rna, or protein and in their ability to demonstrate the cellular localisation of the virus. thus, the biological significance of a positive re ...19921325580
epstein-barr virus genotypes in aids-associated lymphomas are similar to those in endemic burkitt's lymphomas.pcr was used to screen ebv-positive lymphomas from endemic and sporadic burkitt's lymphoma patients, including ebv-positive lymphomas derived from patients with hiv infection. only 10% of sporadic lymphomas from either north america (1/15) or south america (2/14) were associated with the type 2 ebv strain, whereas 50% (8/16) of lymphomas from equatorial africa and 46% (10/22) of hiv-associated lymphomas were positive for the type 2 strain. these data, in conjunction with previous reports, sugges ...19921325581
detection of clonal epstein-barr virus in malignant proliferation of peripheral blood cd3+ cd8+ t cells.epstein barr virus (ebv) dna was detected in a monoclonal proliferation of t cells in a three-year-old girl who presented with a history of fever, hepatosplenomegaly, and generalised lymphadenopathy. the disease ran a rapid, fulminant course and the patient died 11 days after presentation. examination of the blood showed a lymphocytosis of 50 x 10(9)/l with all the cells showing the morphology of large granular lymphocytes. these cells were cd2+3+8+25+. cytogenetic studies showed the presence of ...19921325583
a region of herpes simplex virus vp16 can substitute for a transforming domain of epstein-barr virus nuclear protein 2.epstein-barr virus (ebv) nuclear protein 2 (ebna-2) is essential for ebv-induced b-cell transformation in vitro. ebna-2 contains a 14-amino acid domain that directly activates transcription and is required for transformation. to determine whether another transcriptional activator can substitute for this function, a chimeric virus was constructed that contained a portion of the transcriptional activation domain from the herpes simplex virus vp16 protein inserted in place of the 14-amino acid doma ...19921325641
expression of a family of complementary-strand transcripts in epstein-barr virus-infected cells.a major family of polyadenylylated cytoplasmic transcripts are expressed from the bamhi a-i region of the epstein-barr virus genome, off the strand complementary to that encoding several functions associated with viral replication and the lytic cycle, including the dna polymerase (balf-5). these complementary-strand transcripts (the main one is about 4.8 kilobases long), expressed in all cell types associated with epstein-barr virus, are present at high levels in nasopharyngeal carcinoma tumors. ...19921325642
cd23 and the epstein-barr virus. 19921325660
genetic relationships between bovine herpesvirus 4 and the gammaherpesviruses epstein-barr virus and herpesvirus saimiri.the overall arrangement of genes in the unique central part of the bovine herpesvirus type 4 (bhv-4) genome has been deduced by analysis of short dna sequences. twenty-three genes conserved in at least one of the completely sequenced herpesviruses have been identified and localized. all of these genes encoded amino acid sequences with higher similarity to proteins of the gammaherpesviruses epstein-barr virus (ebv) and herpesvirus saimiri (hvs) than to the homologous products of the alphaherpesvi ...19921325698
interaction of the human polyomavirus, jcv, with human b-lymphocytes.the human polyomavirus, jcv, is the causative agent of the central nervous system demyelinating disease progressive multifocal leukoencephalopathy (pml). the principal target of jcv infection in the central nervous system (cns) is the myelinating oligodendrocyte. however, the site of jcv multiplication outside of the cns and the mechanism by which virus gains access to the brain are not known. recently, jcv infected b-lymphocytes have been demonstrated in pml patients in several lymphoid organs, ...19921325703
immune responsiveness and risk of illness in u.s. air force academy cadets during basic cadet has been proposed, but not confirmed, that environmental stressors alter immune function and increase the risk of viral infection among healthy individuals. we evaluated this hypothesis, examining the relationship among stress, immune function, and illness in 96 first-year u.s. air force academy cadets during orientation and 4 weeks later during the stressful environment of basic cadet training (bct). perceived stress and well-being levels of cadets were assessed via questionnaire. immune res ...19921325771
heterogeneity among human nasopharyngeal carcinoma cell lines for inflammatory cytokines mrna expression levels.using polymerase chain reaction (pcr), we confirmed the expression of interleukin-1 alpha (il-1 alpha) by the human nasopharyngeal carcinoma (npc) cell line c15 without contribution of either human il-1 beta or mouse il-1 alpha in the biological activity previously found in c15. however we showed that il-1 alpha was not expressed in all npcs. il-1 beta and/or tumor necrosis factor (tnf)-alpha genes could also be activated, independently from the number of epstein barr virus (ebv) copies harbored ...19921325786
the biology of hiv-associated lymphomas. 19921325824
direct detection of epstein-barr virus in peripheral blood and comparison of epstein-barr virus genotypes present in direct specimens and lymphoblastoid cell lines established from nasopharyngeal carcinoma patients and healthy carriers in hong means of a pcr assay, ebv was demonstrated directly in peripheral blood of previously infected individuals. the virus was detected in approximately 80% of specimens from ebv-seropositive individuals, but not in cord-blood lymphocytes by this method. when virus present in peripheral blood was compared to that observed directly in npc biopsies or throat washings, it was distinct from that seen in biopsies in 4/15 cases (27%) and from that seen in throat washes in 1/22 cases (5%). the throat-was ...19921325949
control of human b cell tumor growth in severe combined immunodeficiency mice by monoclonal anti-b cell antibodies.severe combined immunodeficiency (scid) mice develop ebv (+)b cell tumors after infusion of ebv(+)b cells or of b cells and ebv. in this study, scid mice were infused with b cell lines derived from three patients who developed a b lymphocyte proliferative disorder after bone marrow or organ transplantation. intraperitoneal injection of 5 x 10(6) b cells induced tumor growth in all mice, leading to death within 60 d. human b cells were identified in spleen and bone marrow by means of immunofluore ...19921326002
kikuchi's disease associated with epstein-barr virus infection. 19921326011
the rapid serological diagnosis of infectious mononucleosis.a total of 121 samples of serum collected from 101 patients was tested to determine the sensitivity and specificity of a commercial latex agglutination test for detecting infectious mononucleosis heterophile antibody, a commercial immunofluorescence test for detecting antibody to epstein-barr virus capsid antigen and a rapid enzyme immunoassay for detecting antibody to epstein-barr virus nuclear antigen. although the epstein-barr virus capsid antigen igm indirect immunofluorescence test proved t ...19921326013
viruses in human tumors--personal reflections.a personal view is presented reflecting some developments in human tumor virology during the past three decades. since the discovery of the epstein-barr virus in 1964 members of additional viral groups have been linked to human tumors, e.g. hepatitis b virus, htlv-1, and a number of different papillomavirus types. it became evident that the virus infection per se is not sufficient and that additional events modifying the host cell genome contribute to the development of virus-linked human cancer ...19921326272
stimulation of human b cells specific for candida albicans for monoclonal antibody production.the stimulation and immortalisation of human peripheral blood b lymphocytes specific for candida albicans antigen were investigated. an in vitro immunisation system was employed which involved pretreatment of mononuclear cells with l-leucyl l-leucine methyl ester which removes the suppressive effects of cd8+ t cells, nk cells and monocytes. the remaining cells, cd4+ t cells, b cells and dendritic cells, were cultured with antigen and a mixture of cytokines. a mixture of il-2, -4 and -6 was found ...19921326299
charge and ph effect on the early events of epstein-barr virus fusion with lymphoblastoid cells (raji).fusion of epstein-barr virus (ebv) with raji cells was measured after exposure of the virus to neutral or low ph, enzymatic modification of the viral spike glycoproteins, or chemical modification of the target membrane. the relief of octadecylrhodamine (r18) fluorescence self-quenching was used to monitor fusion. fusion of ebv with raji cells at ph 5.9 was significantly enhanced compared to that at neutral ph. treatment of raji cells with agents known to modify the surface net charge (trinitrobe ...19921326496
effect of protein kinase c inhibitors with different action mechanisms on epstein-barr virus replication.12-0-tetradecanoyl phorbol-13-acetate (tpa) has been widely known as an activator of epstein-barr virus (ebv) replication and is a direct activator of protein kinase c (pkc). these facts suggest that ebv dna synthesis might at least partly be dependent upon pkc activity. in this report, the effects of two different types of pkc inhibitors on ebv dna synthesis were investigated by slot blot hybridization using a biotin-labeled probe. staurosporine and h-7, inhibitors acting on the catalytic domai ...19921326499
cytotoxic t lymphocytes show hla-c-restricted recognition of ebv-bearing cells and allorecognition of hla class i molecules presenting self-peptides.human ctl have been isolated that show self-restricted recognition of autologous lymphoblastoid cell lines and allorecognition. the lymphoblastoid cell line ligand most likely used a peptide that is expressed in ebv-bearing cells when the virus enters the lytic cycle. this peptide is presented to cd8+ ctl by hla-cw7 molecules. the allogeneic ligand recognized on non-ebv-infected cells is composed of a class i glycoprotein and a naturally selected self-peptide. in previous studies we demonstrated ...19921326579
subclinical pneumonitis due to pneumocystis carinii in a young adult with elevated antibody titers to epstein-barr virus.pneumocystis carinii was recovered from the lungs of a 20-year-old woman in apparent good health who had volunteered to undergo bronchoalveolar lavage (bal) as a normal control subject. total and differential cell counts in the bal fluid revealed a significantly increased number and proportion of t lymphocytes, although the cd4:cd8 ratio was in the normal range. despite the lack of specific antibiotic therapy, in a subsequent lavage no p. carinii were recovered, and the total and differential ce ...19921326586
differential splicing of epstein-barr virus immediate-early rna.a variant form of splicing of rna crossing the epstein-barr virus (ebv) bzlf1 gene was observed in the late productive cycle of ebv. this splice omits the middle exon of bzlf1 and joins the outer two exons of bzlf1 in frame, but the shortened form of bzlf1 protein (z delta) could not be detected in natural ebv infections.19921326670
relapsing acute disseminated encephalomyelitis associated with chronic epstein-barr virus infection: mri findings.a 25-year-old women had a fever, left cervical lymphadenopathy, neurological symptoms and signs, csf pleocytosis and persistent high serum antibodies to the epstein-barr virus (ebv); she had a recurrence 1 year later. she was thought to have relapsing acute disseminated encephalomyelitis associated with chronic ebv infection. mri revealed abnormalities, mainly in the right basal ganglia and left midbrain. at the time of the recurrence, further abnormalities appeared in the opposite basal ganglia ...19921326729
sudden onset focal seizures in a 27-month-old boy. 19921326742
development and characterization of a human monoclonal antibody probably detecting the leukocyte differentiation antigen cd39.a human monoclonal igm antibody, referred to as tu223, has been produced. the reactivity of tu223 was tested in various cells and cell lines by complement-dependent microcytotoxicity test and fluorescence-activated cell sorter analysis. the antigen defined by tu223 was expressed on epstein-barr virus-transformed b-cell lines and on some burkitt's lymphoma cell lines, but was not expressed on normal t cells, b cells or erythrocytes. in addition, expression of the antigen defined by tu223 was also ...19921326791
biochemical analysis of hla-dr antigens using one-dimensional isoelectric focusing gel electrophoresis. 19921326792
expression of the epstein-barr virus latent membrane protein (lmp) in insect cells and detection of antibodies in human sera against this protein.recombinant baculoviruses containing the complete lmp and truncated lmp genes were generated and high levels of the lmp proteins were expressed in spadoptera frugiperda insect cells. a specific rabbit antiserum directed against the n-terminal part of lmp was obtained by immunizing the rabbits with escherichia coli-expressed trpe-n-terminal part of lmp fusion protein. a total of 127 human sera were studied for their immune response to the recombinant full-length lmp. in immunofluorescence analysi ...19921326801
epstein-barr virus in lymphoma. protagonist or passenger? 19921326890
epstein-barr virus-associated anaplastic large cell lymphoma in renal transplant patients.a novel association, epstein-barr virus-positive ki-1+/cd30+ anaplastic large cell non-hodgkin's lymphoma of b-cell phenotype in immunosuppressed renal transplant recipients is reported. case 1 involved an aggressive clinical evolution, whereas case 2 followed a more "benign" clinical course. both lymphomas were epstein-barr virus-positive as assessed by in situ hybridization, southern blot, polymerase chain reaction, and immunohistochemical analysis. both lymphomas contained a single clonal eps ...19921326891
influence of epstein-barr virus genomes on patient survival in hodgkin's previous studies, epstein-barr virus was considered a possible etiologic factor in hodgkin's disease. two hundred twenty-nine cases of hodgkin's disease were investigated for the presence of epstein-barr virus dna using the polymerase chain reaction technique on formalin-fixed, paraffin-embedded lymph node tissue to clarify the clinical importance of the incidence of this genome. in 42 cases (18.3%), genomic dna was not amplifiable. the remaining 187 cases included the following subtypes: lym ...19921326892
primary epstein-barr virus infection with clonal t-cell lymphoproliferation.a case of fatal epstein-barr virus infection in a previously healthy girl who was first found to have severe infectious mononucleosis with spontaneous recovery is reported. because an abnormal immune response to the virus persisted, the disease relapsed, manifesting in cutaneous and pulmonary lesions associated with hemophagocytic syndrome responsible for death. pathologic findings were characterized by polymorphous atypical lymphoid infiltrate, prominent necrosis, and histiocytic hyperplasia. l ...19921326893
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