expression of integrins and other adhesion molecules in epstein-barr virus-transformed b lymphoblastoid cells and burkitt's lymphoma cells.lymphocytes adhere to cells or extracellular matrices to perform functions relating to cytotoxicity, extravasation and tissue localization, as well as modulation of lymphocyte growth and maturation. this adherence is mainly mediated by 3 families of cell-surface adhesion molecules: integrins, immunoglobulin-related molecules and selectins. since variations in the degree of adherence may affect the pathophysiology of lymphoproliferative disorders, the expression of a large number of adhesion mole ...19921317364
[switch mechanisms of disruption of epstein-barr virus latency]. 19921317400
ebv-genome positive monoclonal b cell cerebral lymphoma in a renal allograft recipient following okt3 therapy. 19921317529
demonstration of epstein-barr viral dna in paraffin-embedded specimens of lymphoproliferative syndrome. evidence for a productive infection comparable to lymphoblastoid cell lines.b-cell lymphoproliferative syndromes (lps) occurring in immunodeficient subjects are frequently associated with ebv infections. histology as well as ebv-related serology are not diagnostic, but demonstration of ebv dna in lps suspected lesions might be useful for diagnosis. we studied four autopsied cases of lps that developed in the setting of bone marrow transplantation, with proliferations ranging from poly- to monoclonal. our protocol of dna extraction allowed detection of ebv dna in formali ...19921317554
rejection, herpesvirus infection, and ki-67 expression in endomyocardial biopsy specimens from heart transplant recipients.we examined 164 endomyocardial biopsy specimens from 29 patients who received an orthotopic heart transplant since 1984. rejection was graded according to the hannover classification. non-isotopic in situ dna hybridization was conducted on cryostat sections with biotinylated probes for herpes simplex virus, epstein-barr virus, and cytomegalovirus. serial sections of 126 biopsies were investigated immunohistochemically for the presence of activated t lymphocytes and proliferating cells, with mono ...19921317559
cellular receptor for epstein-barr virus. 19921317595
molecular epidemiology of burkitt's lymphoma from south america: differences in breakpoint location and epstein-barr virus association from tumors in other world regions.we have previously shown that the endemic (african) and sporadic (north american) forms of burkitt's lymphoma (bl) differ at a molecular level. we have now extended our studies to the molecular epidemiology of bl in south america, specifically to two climatic regions: temperate (argentina and chile) and tropical (brazil). we have examined the patterns of chromosomal breakpoint locations in 39 tumors with respect to geography and epstein-barr virus (ebv) association. the result of these analyses ...19921317726
hodgkin's disease.the epidemiological features of hodgkin's disease (hd) suggest that it is a heterogeneous condition which may have different aetiologies in different age groups. the risk factors for the development of hd in young adults suggest that delayed exposure to a common infectious agent may be involved in this age group. seroepidemiological studies have shown that hd patients have elevated antibody titres to epstein-barr virus (ebv) and the elevated titres have been shown to precede the diagnosis of hd. ...19921317729
non-hodgkin's lymphomas and paraproteinaemias. 19921317730
early activation of the proto-oncogene c-fgr during epstein-barr virus an attempt to clarify the chronological relationships between epstein-barr virus (ebv) infection, b cell immortalization and c-fgr activation, we evaluated for the presence of ebv-determined nuclear antigen (ebna), cellular dna synthesis and expression of c-fgr-specific rna following infection of human peripheral blood lymphocytes with b95-8 ebv. high expression of c-fgr was observed prior to ebna detection and cellular dna synthesis in ebv-infected cells. these results suggest that activatio ...19921317747
[hodgkin's disease in hiv infection--detection of epstein-barr virus dna in tongue epithelium and lymphoma].we present an hiv-infected patient with lymphadenopathy syndrome in whom an unusually aggressive case of hodgkin's disease developed. examination of tissue excised from the lymphoma and of epithelial cells scraped from the tongue of the patient revealed epstein-barr virus (ebv). the relationship between an enhanced replication of ebv in the epithelium of the tongue and the elevated frequency of hodgkin's lymphomas containing ebv-dna among hiv-infected patients is discussed.19921317834
epstein-barr virus dna in nasopharyngeal carcinomas from chinese patients in hong investigate the presence of epstein-barr virus (ebv) in cases of nasopharyngeal carcinoma (npc) in chinese patients living in hong kong.19921317885
monoclonal proliferation of t cells containing epstein-barr virus in fatal mononucleosis. 19921317943
a simple and efficient mammalian gene expression system using an ebv-based vector transfected by electroporation in g2/m phase. 19921317956
localization of epstein-barr viral genomes in angiocentric immunoproliferative lesions.epstein-barr virus has been demonstrated in angiocentric immunoproliferative lesions. however, these studies, which have used the polymerase chain reaction technique or southern blot analysis, have not revealed the identity of the cell population harboring the virus. to address this issue, we analyzed 12 angiocentric immunoproliferative lesions (14 biopsy specimens) for epstein-barr virus rna using a sensitive and specific in situ hybridization method. epstein-barr virus rna was detected in five ...19921318000
human immunodeficiency virus activates c-myc and epstein-barr virus in human b lymphocytes.we have established a line of malignantly transformed human b cells by infecting purified primary b lymphocytes with human immunodeficiency virus type 1 (hiv-1). this line, termed b-hiv1, may serve as a model system for a subset of aids-related b-cell lymphomas in which the transformed phenotype may be initiated and/or maintained through an hiv-1 gene product. the b-hiv1 line contains both epstein-barr virus (ebv) and hiv-1 genomes. in addition, the c-myc gene is expressed at levels 10 to 20 tim ...19921318011
expression of immunoglobulin heavy chain variable gene (vh) in b-chronic lymphocytic leukemia (b-cll) and b-prolymphocytic leukemia (b-pll) cell lines. "restricted" usage of vh3 family. 19921318012
prevalence of epstein-barr virus dna in different t-cell lymphoma entities in a european population.the epstein-barr virus (ebv) has been classically associated with nasopharyngeal carcinoma and burkitt's lymphoma, a monoclonal b-cell non-hodgkin's lymphoma. since the ebv genome has also been found in post-transplant lymphomas and lymphomas arising in individuals infected with the human immunodeficiency virus, evidence has now accumulated that ebv might be the initiator of a multi-step process leading from polyclonal b-cell hyperplasias to monoclonal lymphoma. in a retrospective study of 60 t- ...19921318268
identification of the 12-o-tetradecanoylphorbol-13-acetate-responsive enhancer of the ms gene of the epstein-barr virus.we previously located two 12-o-tetradecanoylphorbol-13-acetate (tpa)-responsive enhancers, mstre-i and mstre-ii, in the upstream sequence of the ms gene of epstein-barr virus (liu, q., and summers, w.c. (1989) j. virol. 63, 5062-5068). the core sequence of the mstre-i enhancer is now determined to be between -718 and -708 of the upstream sequence of the ms gene. the activity of the enhancer is also sensitive to its immediate surrounding sequence on either side. a single copy of a 30-base pair (b ...19921318308
infection of peripheral blood mononuclear cells by herpes simplex and epstein-barr viruses. differential induction of interleukin 6 and tumor necrosis factor-alpha.infection by herpesviruses can result in profound immunosuppressive or immunomodulatory effects. however, no significant information is available on the effect of such infections on the production of immunoregulatory cytokines. we studied the kinetics of production of two monocyte-derived cytokines, interleukin 6 (il-6) and tumor necrosis factor-alpha (tnf alpha), induced by epstein-barr virus (ebv) and herpes simplex virus type 1 (hsv-1) in peripheral blood mononuclear cell cultures and in frac ...19921318324
virus-specific antibodies to epstein-barr virus, varicella-zoster virus and rubella virus in renal transplant patients with cytomegalovirus infections.renal transplant patients with primary and recurrent cytomegalovirus (cmv) infection had higher antibody titres to epstein-barr virus viral capsid antigen (ebv-vca-igg) before and after transplantation than healthy blood donors. the geometric mean titres (gmt) of ebv-vca-igg were higher in renal transplant patients without cmv infection than in renal transplant patients with cmv infection. four-fold or greater rises in ebv-vca-igg antibody were detected in six patients and a similar rise in anti ...19921318341
prevalence of epstein-barr virus and human papillomavirus in oral mucosa of hiv-infected patients.epstein-barr virus (ebv) has been implicated in the genesis of oral hairy leukoplakia (ohl). initially, ohl was also associated with human papillomavirus (hpv) as evidenced by staining with antiserum to papillomavirus common structural antigens and reports of two hpv-positive ohl as detected by in situ dna hybridization. the aims of this study were to determine the prevalence of ebv and hpv dna in ohl and normal oral mucosa and to explain the basis for the staining of ohl tissues with antibodies ...19921318379
phenotypes of epstein-barr virus lmp1 deletion mutants indicate transmembrane and amino-terminal cytoplasmic domains necessary for effects in b-lymphoma cells.the epstein-barr virus (ebv) latent infection membrane protein 1 (lmp1) has previously been shown to cause ebv-negative b-lymphoma cells to grow in large clumps and to alter expression of surface activation and adhesion molecules (d. wang, d. liebowitz, f. wang, c. gregory, a. rickinson, r. larson, t. springer, and e. kieff, j. virol. 62:1473-4184, 1988; f. wang, c. gregory, c. sample, m. rowe, d. liebowitz, r. murray, a. rickinson, and e. kieff, j. virol. 64:2309-2318, 1990). in order to identi ...19921318423
characterization of ebv-positive lymphoblastoid cell lines obtained from hiv seropositive patients with or without lymphomas.epstein-barr-virus- (ebv-) positive lymphoblastoid cell lines (lcls) spontaneously arising in vitro were obtained from the peripheral blood of six hiv-seropositive patients and from the peripheral blood and the bone marrow of one patient (lam) with aids and lymphoma. the lcls from hiv-seropositive patients had phenotypic, cytogenetic, and biological characteristics indistinguishable from those of normal lcls obtained by infecting b cells with ebv in vitro. the lcls from lam patient comprised com ...19921318463
lymphoma induction by human b cells in scid mice.lymphoma development was studied in scid mice injected i.p. with pbmc from ebv-positive donors. most injected mice developed oligo/monoclonal b-cell tumors within 4 months after the inoculation; ebv genome was found in tumor cells. removal of t lymphocytes from the injected cell populations prevented lymphoma development in all mice, suggesting that t-cell-derived factors are involved in the expansion of the latently ebv-infected b-cell population within the immunodeficient host.19921318472
induction of tumorigenicity and plasmacytoid differentiation in ebv-b cells by expression of exogenous interleukin-6 or il-6 receptor genes. 19921318473
lymphoma: an opportunistic neoplasia of aids. 19921318474
morphological and phenotypical changes in ebv positive lymphoblastoid cells infected by hiv-1.human immunodeficiency virus type 1 (hiv-1) infects cd4+ t lymphocytes and various other cell types, including b cells. since hiv-1 seropositive individuals have high numbers of b cells carrying epstein-barr virus (ebv), and are at high risk for development of ebv-associated lymphoproliferative diseases, we studied the mode of hiv-1 infection in four ebv-positive lymphoblastoid b-cell lines (lcls) as well as some molecular and biological features of the b cells infected by both viruses. we found ...19921318475
the epidemiology of human virus-associated hematologic malignancies. 19921318477
factors in the development of aids-related lymphomas. 19921318478
characteristics of epstein-barr virus transformed b cell lines from patients with alzheimer's disease and age-matched controls.the characteristics of b cell lines isolated from patients with alzheimer's disease (ad) and age-matched controls were investigated after having been transformed by epstein-barr virus (ebv). after isolation of mononuclear blood cells and in vivo or in vitro ebv infection, 35 and 21 lymphoblastoid cell lines (lcls) were generated from 19 patients with ad (mean age 79.4 years) and 21 age-matched controls (mean age 80.0 years), respectively. b lymphocytes from ad patients were immortalised more eas ...19921318479
targeted gene disruption in epstein-barr virus.we report the development of a method that should allow the insertion of a selective marker into any region of the epstein-barr virus (ebv) genome of strain b95-8 through homologous recombination with plasmids. in this method, ebv recombinants are isolated as g418-resistant, immortalized b-cell clones or as g418-resistant, latently infected subclones of burkitt lymphoma cell lines. the presence of the productive replication origin of ebv, orilyt, on the plasmid was found to increase the number o ...19921318603
a survey of epstein-barr virus gene expression in sporadic non-hodgkin's lymphomas. detection of epstein-barr virus in a subset of peripheral t-cell lymphomas.this study analyzes the association of epstein-barr virus (ebv) with non-hodgkin's lymphoma (nhl) arising in patients without pre-existing overt immunodeficiency. the authors examined 201 lymphomas (105 high-grade b-cell, 82 peripheral t-cell, 7 high-grade non-b-cell, non-t-cell, and 7 hairy-cell leukemia) for ebv gene expression by immunohistologic procedures using monoclonal antibodies to ebv latent, immediate early, and replicative infection antigens. transformation-associated ebv latent memb ...19921318639
chronic fatigue syndrome. a review from the general practice perspective.there is no doubt that the chronic fatigue syndrome exists. it is a condition that is debilitating and of unknown cause. research into chronic fatigue syndrome demonstrates possible psychiatric or organic causes. the truth may be somewhere in between. evidence for the existence of an ongoing chronic infection is now not convincing. treatment should be based on supportive counselling, explanation, psychiatric help (both pharmacological and non pharmacological) and a graded programme of increased ...19921318714
[the presence of the epstein-barr virus in waldeyer's lymphatic ring during the acute phase of infectious mononucleosis].the aim of our research was to detect the existence of virus epstein-barr genome in the palatine- and the nasopharyngeal tonsil obtained from six patients during the acute phase of the infectious mononucleosis. the presence of ebv-dna was demonstrated with the help of nucleic acid hybridization techniques (southern-blot and in situ hybridization) in the two components of the lymphoepithelial ring of the throat. the pathogenesis and the therapeutic implications of these findings are discussed.19921318729
epstein-barr virus integration in human lymphomas and lymphoid cell lines.epstein-barr virus (ebv) is maintained as an episome in most infected cells. the presence of fused terminal restriction enzyme fragments distinguishes the circular dna form from the linear virion form.19921318776
trisomy 12 in epstein-barr virus-transformed lymphoblastoid cell lines of normal individuals and patients with nonhematologic malignancies.karyotypes of 36 lymphoblastoid cell lines established by epstein-barr virus (ebv) transformation of peripheral blood lymphocytes (pbl) of eight normal individuals and 28 patients with various nonhematologic malignancies were analyzed. in seven lines (19.4%), cells with trisomy 12 were noted, with clonality in two of these lines. in two of 11 metaphases with such trisomy, chromosome 12 was involved in structural rearrangements [t(8;12)(q12;p12) and t(12;12)(q11;q24)]. no cells with trisomy 12 we ...19921318781
enhanced levels of radiation-induced g2 phase delay in ataxia telangiectasia heterozygotes.ataxia telangiectasia (at) is a multiform genetic disease characterized by immunodeficiency, cerebellar abnormalities, and cancer predisposition. heterozygotes also have an increased risk of developing several different cancers. it has been estimated that as many as 18% of all patients with breast cancer, the cancer most clearly associated with at heterozygotes, may be carriers of the at gene. we describe an assay for at heterozygotes that relies on the previous observation that cells from at ho ...19921318783
studies on inhibitors of skin tumor promotion. xi. inhibitory effects of flavonoids from scutellaria baicalensis on epstein-barr virus activation and their anti-tumor-promoting search for possible anti-tumor-promoters, fourteen flavones obtained from the root of scutellaria baicalensis were examined for their inhibitory effects on the epstein-barr virus early antigen (ebv-ea) activation by a short-term in vitro assay. among these flavones, 5,7,2'-trihydroxy- and 5,7,2',3'-tetrahydroxyflavone showed remarkable inhibitory effects on the ebv-ea activation, and the effect of the latter on raji cell cycle was also examined by flow cytometer. these two flavones exhibited ...19921318792
modulatory effects of epstein-barr, herpes simplex, and human herpes-6 viral infections and coinfections on cytokine synthesis. a comparative study.herpesviruses such as ebv, hsv, and human herpes virus-6 (hhv-6) have a marked tropism for cells of the immune system and therefore infection by these viruses may result in alterations of immune functions, leading at times to a state of immunosuppression. we report the results of a comparative study in which we found that ebv, hsv-1, and hhv-6 act differentially on the immune system with regard to their effect on the synthesis of il-1 beta, il-6, and tnf-alpha, i.e., three immunoregulatory cytok ...19921318897
ebv increases phosphoinositide kinase activities in human b determine whether ebv affects phosphoinositide kinase activities of human b cells, we compared the activities between ebv- and ebv+ human b cell lymphoma lines. the two types of human b cells contained both phosphatidylinositol (ptdins) 4-kinase and phosphatidylinositol 4-phosphate (ptdins(4)p) kinase activities irrespective of the presence of ebv. however, both activities were increased in ebv+ cells compared to ebv- cells. the increases were associated with neither altered km values for sub ...19921318898
cell growth effects of epstein-barr virus leader protein.b lymphoblastoid cell lines immortalized with p3hr1/633 epstein-barr virus (ebv), which has a deletion in the ebv nuclear antigen leader protein (ebna-lp) gene, were transfected with a vector expressing wild-type ebna-lp. the ebna-lp transfectants grew out faster under g418 selection than control cells but expression of ebna-lp made no significant difference to growth rate or saturation density of the resulting established cell lines. when the cells expressing ebna-lp were allowed to grow to sat ...19921318948
[igg and iga antibodies to the epstein-barr virus and nasopharyngeal carcinoma]. 19921318986
comparison of ebv-infectivity measured by ebna-induction and immortalization of human b-cells.epstein-barr virus (ebv) infectivity was measured by two different systems. one was ebv-specific antigen (ebna) induction in ebv-negative bjab cells, the other was immortalization of human peripheral blood b-lymphocytes upon ebv infection. because the sensitivity of the ebna-induction assay, which takes 2-3 days, was similar with that of b-cell immortalization, which takes 6-8 weeks, the former can be used for the rapid and convenient determination of ebv-infectivity.19921319181
a novel human lymphoma cell line (deglis) with dual b/t phenotype and gene rearrangements and containing epstein-barr virus genomes.we report a new and apparently unique human lymphoma cell line termed deglis. the line was established from a polymorphic centroblastic lymphoma. the cell line and its source carry a dual b-cell and t-cell phenotype and epstein-barr virus (ebv) genomes. simultaneous expression of b-cell (cd19+, cd20+, cd23+, cd37+) and t-cell (cd2+, cd3+/-, cd7+, cd43+) antigens, activation antigens (cd30+, cdw70+) as well as cd68+, a macrophage-associated antigen, was observed on the cell line and its source. g ...19921319235
pathogenetic role of epstein-barr virus in malignant lymphomas that develop in immunocompromised patients. 19921319238
acquired immunodeficiency syndrome-related lymphoma. 19921319239
studies on the relationship between chronic fatigue syndrome and epstein-barr virus in japan.among 1,153 consecutive patients, 22 patients (1.9%) who complained of chronic fatigue for a period of over 6 months without detectable causes were studied. ten patients (0.86%) satisfied the criteria of chronic fatigue syndrome (cfs) and were classified to be definite cases of cfs. the other patients were classified as probable cases. in order to clarify the role of epstein-barr virus (ebv) as a cause of cfs, we measured various antibodies for ebv. the definite cases had significantly higher ti ...19921319246
antibodies to histones in infectious mononucleosis.a polyspecific human monoclonal (auto)antibody, isolated from a patient in the acute phase of infectious mononucleosis, was found to react with all subfractions (h1, h2a, h2b, h3 and h4) of histones. this finding prompted us to study the occurrence of antibodies to histones in sera of patients with infectious mononucleosis. it was found that igm binding to histones was detectable both in control and patient sera; however, sera from patients showed binding values of igm antibodies to histones sig ...19921319396
detection of occult nasopharyngeal primary tumours by means of in situ hybridization.detection of nasopharyngeal carcinoma primaries in patients presenting with neck node metastases may sometimes demand considerable efforts. by using the 'in situ hybridization' technique, we manage to identify the epstein-barr virus in neck metastases secondary to nasopharyngeal carcinomas. we propose that such identification in neck node metastases where the primary lesion is unknown indicates a nasopharyngeal primary.19921319444
identification of target antigens for the human cytotoxic t cell response to epstein-barr virus (ebv): implications for the immune control of ebv-positive malignancies.epstein-barr virus (ebv), a human herpes virus with oncogenic potential, persists in b lymphoid tissues and is controlled by virus-specific cytotoxic t lymphocyte (ctl) surveillance. on reactivation in vitro, these ctls recognize ebv-transformed lymphoblastoid cell lines (lcls) in an hla class i antigen-restricted fashion, but the viral antigens providing target epitopes for such recognition remain largely undefined. here we have tested ebv-induced polyclonal ctl preparations from 16 virus-immun ...19921319456
a monkey model for epstein barr virus-associated lymphomagenesis in human acquired immunodeficiency syndrome.high-grade malignant nonhodgkin's lymphomas--five lymphoblastic, three pleomorphic, and two immunoblastic--developed in 10/25 cynomolgus monkeys (macaca fascicularis) followed for up to 746 d after infection with simian immunodeficiency virus, strain sivsm. these lymphomas were shown to be associated with an epstein-barr (eb)-like cynomolgus b-lymphotropic herpesvirus (cblv) by electron microscopy, by southern blot hybridization with probes against human ebv, and by the expression of antigens co ...19921319458
potential role of epstein-barr virus in sjögren's syndrome and rheumatoid the pathogenesis of sjögren's syndrome (ss), a role for epstein-barr virus (ebv) has been suggested because; (a) ebv is present in salivary gland epithelial cells of healthy individuals, and exaggerated immune responses against ebv could play a role in the destruction of salivary glands in ss; (b) ss salivary gland biopsies contain increased levels of ebv dna compared to normal salivary glands, indicating viral reactivation and inability of lymphoid infiltrates to control ebv replication in p ...19921319486
isolation of epstein-barr virus genomes using pulse-field gel electrophoresis. 19921319577
[virus and cancer].there are at least four viruses tightly associated with human cancer: htly-i and htly-ii with certain leukemias, ebv with lymphomas, bhv with hepatocarcinomas and hpv with genital cancer. in this work we discuss some evidences indicating these associations; in particular we emphasize the characteristics of human papillomavirus (hpv), due to its growing importance in the development of uterine-cervix carcinoma and the mortality of mexican women. the low percentage of infected individuals that dev ...19921319613
[macrophage activation syndrome and dysgammaglobulinemia. role of the epstein-barr virus].the case of a five-year-old boy with macrophage activation syndrome and epstein-barr virus infection is reported. several unusual features were found, including an early major increase in iga levels, persistent oligoclonal gammapathy, and delayed development of anti-ea antibodies accompanied with an increase in anti-vca antibodies. despite the negative family history, an immune deficit and purilo syndrome are discussed as possible diagnoses in this child.19921319695
herpes simplex virus-1-specific human cytotoxic t lymphocytes are induced in vitro by autologous virus-infected mononuclear cells.a new technique for in vitro activation of cytotoxic t lymphocytes (ctls) specific for herpes simplex virus type 1 (hsv-1) is described. autologous phytohemagglutinin (pha)-activated, hsv-1-infected peripheral blood mononuclear cells (pbmc) were used, after fixation with 1% paraformaldehyde, to activate virus-specific ctls in short-term cultures. the same unfixed pbmc were used as target cells in the cytotoxicity assay. by using this technique high levels of hsv-1-specific cytotoxic activity (50 ...19921319712
epstein-barr virus-associated primary b-cell lymphoproliferative disorder of the cerebellum in an immune competent man.the role of the epstein-barr virus (ebv) in lymphoproliferative lesions has been widely accepted. most of these lesions occur in patients who have deficiencies in their immune status. lymphomatoid granulomatosis (lg) is a lymphoproliferative disorder originally characterized as an angiocentric, necrotizing, pleomorphic infiltrate of mononuclear cells. the etiology of lg is unknown. it was originally hypothesized that lg may represent an unusual lymphoid response to an infective organism, possibl ...19921319819
[nasopharyngeal carcinoma (npc) mortality rate in two districts of guangdong province and their relation to iga antibody level against eb virus capsid antigen].based upon the retrospective investigation data, the npc mortality rate in the pearl river delta was found to be twice as high as that of chaoshan district. the positive rate of iga/vca antibody in the pearl river delta was also found to be significantly higher than that in the chaoshan district by viro-serological screening. the iga/vca antibody positive rate and mortality rate were concomitantly increased with age in the bracket of 30-59. accordingly, the authors assume that activation of infe ...19921319889
impaired long-term t cell immunity to epstein-barr virus in patients with nasopharyngeal carcinoma.the long-term t cell immunity to epstein-barr virus (ebv) is considered to play an important role in suppressing proliferation of ebv-infected b cells and outgrowth of ebv-associated tumors. it can be manifested and quantified by the ebv-induced focus regression assay. in the present study, we examined the strength of t cell immunity to ebv in patients with nasopharyngeal carcinoma (npc) and other cancers originating from the head and neck region. in contrast to patients with other types of canc ...19921319984
purification and quantification of recombinant epstein-barr viral glycoproteins gp350/220 from chinese hamster ovary cells.truncated epstein-barr virus (ebv) membrane antigen gp350/220 (ebv-ma) lacking the membrane anchor was expressed and secreted into the medium of recombinant chinese hamster ovary cells that had been cultured in plasmapur hollow-fibre modules using defined serum-free medium. the ebv-ma in the medium was concentrated by 70% (w/v) ammonium sulphate precipitation and subsequently purified by immunoaffinity chromatography using an anti-ebv-ma (ebv.0t6) monoclonal antibody (mab) column. adsorbed antig ...19921320046
detection of ebv dna in post-nasal space biopsy tissue from asymptomatic ebv-seropositive individuals.the association between ebv and nasopharyngeal carcinoma (npc) has been well documented although the precise role of the virus in the genesis of the tumour is not understood. we undertook this study to examine the prevalence of ebv infection in nasopharyngeal tissue obtained from 33 healthy individuals not considered to be at risk of developing npc. using polymerase chain amplification (pcr) and in situ hybridization we have identified ebv dna in 70% (23/33) of the tissues examined. our data dem ...19921320096
detection of multiple epstein-barr viral strains in allogeneic bone marrow transplant recipients.we have previously shown in 3 allogeneic bone-marrow transplant (bmt) recipients that complete replacement of recipient marrow was associated with the elimination of the pretransplant epstein-barr virus (ebv) strain of the recipient. to study the kinetics of ebv elimination and reinfection in more detail, we have performed a longitudinal study of bmt recipients combining serology, virus isolation from mouthwashes and peripheral blood, and ebv strain characterization. oropharyngeal ebv excretion ...19921320097
[a basic study on the association of epstein-barr virus with nasopharyngeal carcinoma--detection and genotypic analysis of epstein-barr virus associated with nasopharyngeal carcinoma in japanese patients].epstein-barr virus (ebv) is known to be associated with two human malignant diseases, nasopharyngeal carcinoma (npc) and endemic burkitt's lymphoma. in this study, the genotypes of ebv in tissues from 13 npc patients in japan were analyzed by southern blot hybridization using ebv genome fragment probes. ten of the cases contained reiterated sequences (ebv bamhi-h, -b1*, -k fragments), showing that only one genotype was detected in each specimen. one of these had a bamhi fragment containing a fus ...19921320117
psychosocial modulation of antibody to epstein-barr viral capsid antigen and human herpesvirus type-6 in hiv-1-infected and at-risk gay men.we investigated the effects of two behavioral interventions--aerobic exercise and cognitive behavioral stress management (cbsm)--on epstein-barr virus viral capsid antigen (ebv-vca) and human herpesvirus type-6 (hhv-6) antibody modulation in 65 asymptomatic gay men measured at several time points in the 5 weeks preceding and following notification of their human immunodeficiency virus-type 1 (hiv-1) serostatus. after accounting for potential immunomodulatory confounds, we found that hiv-1 seropo ...19921320279
newest human herpesvirus (hhv-6) in the guillain-barré syndrome and other neurological investigate the presence of human herpesvirus-6 (hhv-6) in patients affected by guillain-barré syndrome (gbs) and by other neurological diseases (ond), we examined by indirect immunofluorescence analysis (ifa) the sera and cerebrospinal fluid (csf) from 28 gbs and 63 ond. moreover, we tested 150 blood donors (bd) to appreciate the diffusion of hhv-6 infection in the italian adult healthy population. we found a significantly higher titre of antibody to hhv-6 in the gbs patients compared with o ...19921320318
asparagine-linked sugar chains of plasma membrane glycoproteins from healthy and common variable immunodeficiency b lymphoblastoid cell lines.asparagine-linked sugar chains of plasma membrane glycoproteins, which are formed by glycosylation during b cell maturation, were examined with b lymphoblastoid cell lines (lcls) transformed by epstein-barr virus derived from healthy controls and patients with common variable immunodeficiency (cvi). both two patients with cvi showed hypogammaglobulinemia and impaired b cell functions. lcls from healthy controls and the patients showed cd19+ and hla/dr+ in the cell surface and secreted igm. in bo ...19921320390
recombinant antigens in viral diagnosis.dna-fragments coding for a variety of different viral antigens have been cloned and expressed in e. coli. selected purified recombinant antigens were used for detection of specific antibodies by the means of elisa technique. this approach has been used for the development of four different elisa's for the detection of hiv- and ebv-specific antibodies.19921320439
clonal frequency analysis of b cells producing pathogenic anti-dna antibody-associated idiotypes in systemic lupus order to identify the mechanism responsible for autoantibody production in systemic lupus erythematosus (sle), b cell repertoires associated with anti-dna idiotypes were explored by a limiting dilution analysis using epstein-barr virus (ebv) transformation methods and elisa spot assays. the frequencies of b cell clones producing antibodies to dna and to conventional antigens, tetanus toxoid, dinitrophenyl, or keyhole limpet hemocyanin were higher in active sle compared to those in inactive sl ...19921320474
use of enzyme-linked immunosorbent assays with chimeric fusion proteins to titrate antibodies against epstein-barr virus nuclear antigen 1.two new enzyme-linked immunosorbent assays (elisas) with chimeric fusion polypeptides for the detection of human antibodies specific to epstein-barr virus nuclear antigen 1 (ebna-1) are described. one is an indirect elisa with affinity-purified beta-galactosidase-ebna-1 fusion protein as the antigen. the other is a "sandwich" assay based on the use of anti-beta-galactosidase antibody to capture beta-galactosidase-ebna-1 fusion proteins in bacterial extracts. a good correlation was shown between ...19921320628
epstein-barr virus infection: aetiological role versus coincidental event in hodgkin's disease. 19921320671
epstein-barr virus-associated lymphoproliferative disorders.herein we have provided a panorama of the clinical, histopathologic, and molecular biologic mechanisms of ebv-induced lpd particularly in immunosuppressed individuals. a listing of ebv-related diseases is shown in table 4. we have stressed the frequent need to use multiple diagnostic methods for detecting ebv genome, particularly in immunodeficient patients who may fail to mount antibody responses to ebv. given that we now recognize some of the immunocompromised patient populations at high risk ...19921320711
demonstration of epstein-barr virus genome in neoplastic cells of hodgkin's disease by in situ hybridization, in paraffin-embedded tissue using biotinylated probes. a study of 46 cases.paraffin-embedded tissue specimens from 46 cases of hodgkin's disease (hd) were studied by in situ hybridization with biotinylated probes for the presence of ebv genomes. ebv specific dna sequences were detected in the nuclei of reed-sternberg (rs) and hodgkin cells (h), in 14 of these 46 cases. there was no correlation between positive hybridization and morphological subtype or site of tumor. by demonstrating an exclusive localization of the viral dna in the tumor cells of hd, our study adds to ...19921320758
epstein-barr virus serology and isoelectrofocusing pattern of serum immunoglobulins in cyclosporin or placebo-treated type i diabetics.epstein-barr virus (ebv) serology was serially studied for a period of 18 months in 49 recently diagnosed type i diabetics randomly assigned to receive cyclosporin (csa) (n = 25) or placebo (n = 24). additionally, isoelectrofocusing of serum (ief) was serially performed in 59 csa- and 38 placebo-treated diabetics, over a 36 month period (687 sera) in order to detect restriction of heterogeneity of circulating immunoglobulins. patients were continuously treated with csa at a dose of 7.5-10 mg/day ...19921320887
antibodies against polypeptides of purified epstein-barr virus in sera from patients with connective tissue diseases.antibodies to epstein-barr virus (ebv) were investigated in patients with systemic lupus erythematosus (sle) and mixed connective tissue disease (mctd) by immunoblotting using purified virus. compared with sera from epstein-barr virus seropositive healthy individuals who served as control, sera from patients less frequently recognized several polypeptides. in particular, a 100 kda envelope polypeptide was recognized by 92% of healthy subjects and only 11% of patients (p less than 0.001). on the ...19921320888
pcr diagnostics of herpes-virus-group infections. 19921320899
epstein-barr virus-carrying cells in hodgkin's disease. 19921320951
apoptotic cell death of primed cd45ro+ t lymphocytes in epstein-barr virus-induced infectious mononucleosis.the expansion of activated t cells, characterized by the expression of cd45ro as well as hla-dr antigens, is a central feature in acute infectious mononucleosis (im) induced by primary infection of epstein-barr virus (ebv). however, the fate of these activated t cells in this disease is not clearly understood. we found that, on simple culture, a large proportion of t cells isolated from acute im patients died rapidly, but only a few t cells from normal individuals did. morphologic observations a ...19921320953
distribution and phenotype of epstein-barr virus-harboring cells in hodgkin's disease.distribution and phenotype of epstein-barr virus (ebv)-harboring cells were determined in hodgkin's disease (hd) biopsies by in situ hybridization with [35s]-labeled rna probes specific for the small ebv-encoded nuclear rnas, eber1 and eber2, in some instances preceded by immunohistology for cd20, cd30, cd45ro, and cd68 antigens, the t-cell receptor beta-chain, and latent membrane antigen (lmp) of ebv. twenty-three of 46 hd cases displayed eber transcripts in all hodgkin and reed-sternberg (h-rs ...19921320954
interleukin-6 production in high-grade b lymphomas: correlation with the presence of malignant immunoblasts in acquired immunodeficiency syndrome and in human immunodeficiency virus-seronegative patients.the mechanisms leading to malignant cell proliferation may differ between the different histologic forms of high-grade non-hodgkin's lymphomas. to analyze the potential role of interleukin-6 (il-6) as a growth factor for lymphomatous cells in these different forms, the in situ production of this cytokine was analyzed in lymphomatous samples taken from 24 patients, 18 of whom were human immunodeficiency virus (hiv) infected. eleven burkitt's lymphomas (bls), seven diffuse large-cell lymphomas, an ...19921320956
biochemical characterization of epstein-barr virus nuclear antigen 2a and an associated atpase activity.a partial purification of the epstein-barr-virus nuclear antigen 2a (ebna 2a) protein from the epstein-barr-virus-infected lymphoblastoid cell line, cherry, has been designed. the main purification step was immunoaffinity chromatography, based on the mab, 115e, directed towards the carboxy terminus of ebna 2a. this was followed by chromatography over a blue sepharose column. according to silver-stained sds/page, ebna 2a was estimated to be 20% pure. the purified fractions contained an atpase act ...19921321048
identification of homologues to the human cytomegalovirus us22 gene family in human herpesvirus 6.the sequence of 10079 bp corresponding to the overlapping sali h and smai g restriction fragments of the genome of human herpesvirus 6 (hhv-6) strain u1102 was determined. the sequence contains six complete open reading frames (orfs) and two incomplete orfs located at the 5' and 3' ends of the sali h and smai g fragments respectively. seven of these orfs have recognizable homologues only in the beta-herpesvirus human cytomegalovirus (hcmv), no obvious counterparts being detectable in the genomes ...19921321206
simian virus 40 (sv40) large t antigen-dependent amplification of an epstein-barr virus-sv40 hybrid shuttle vector integrated into the human hela cell genome.we analysed the dna rearrangements that occurred during the integration and amplification of an epstein-barr virus (ebv)-simian virus 40 (sv40) hybrid shuttle vector in human cells. the human hela cell line was episomally transformed with the ebv-sv40 p205-gti plasmid. after a 2 month culture in a selective medium, a hela cell-derived population (h-g1 cells) was obtained in which the p205-gti vector was integrated as a single intact copy deleted in the ebv latent origin of replication (orip). se ...19921321208
rna polymerase iii-transcribed eber 1 and 2 transcription units are expressed and hypomethylated in the major epstein-barr virus-carrying cell types.the genome of epstein-barr virus (ebv) codes for two non-translated small rna molecules, eber 1 and 2. we found that both ebers are expressed in the major ebv-carrying cell types, group i and iii burkitt's lymphoma (bl) cell lines, lymphoblastoid cell lines (lcls) and in two nude mouse-passaged nasopharyngeal carcinoma (npc) tumours. the relative amount of eber 1 and eber 2 varied in different host cells but did not correlate with the cellular phenotype. the eber coding and flanking sequences we ...19921321209
identification of the glycoprotein h gene of murine cytomegalovirus.partial sequencing of the hindiii c fragment of murine cytomegalovirus (mcmv) revealed an open reading frame of 2172 nucleotides in length encoding a 724 amino acid protein with a predicted mr of 80.4k. analysis of the predicted amino acid sequence revealed homology with glycoprotein h (gh) from a number of other herpesviruses. mcmv gh showed strongest amino acid identity with human (h) cmv and human herpesvirus 6 gh, and less identity with the gh protein sequences of epstein-barr virus, varicel ...19921321219
the epstein-barr virus nuclear protein 1 promoter active in type i latency is autoregulated.the only member of the epstein-barr virus family of nuclear proteins (ebnas) expressed during type i and type ii latent infections is ebna-1. this is in contrast to type iii latency, during which all six nuclear proteins are expressed from a common transcription unit. the exclusive expression of ebna-1 during type i and ii latency is mediated through a recently identified promoter, fp. the objective of this study was to characterize fp in the burkitt lymphoma cell background, where it is known t ...19921321268
epstein-barr virus bzlf1 transactivator is a negative regulator of jun.the epstein-barr virus bzlf1 protein that can induce the lytic cycle in latently infected cells is a transcription factor partially homologous to fos and binds not only the canonical tpa (tetradecanoyl phorbol acetate)-responsive element (tre) site but also sequences deviating from the tre consensus sequence. thus, expression of cellular genes regulated by ap-1, including the autoregulated ap-1 family, should be affected by bzlf1. however, induction of only fos by bzlf1 was observed in a gel mob ...19921321269
transcriptional synergy by the epstein-barr virus transactivator zebra.zebra is an epstein-barr virus (ebv) transcriptional activator that mediates a genetic switch between the latent and lytic states of the virus by binding to the promoters of genes involved in lytic dna replication and activating their transcription. a computer survey revealed that 9 of 23 potential or known zebra-responsive ebv genes contained two or more upstream binding sites; this suggested that zebra can stimulate transcription synergistically. by using a series of synthetic promoters bearin ...19921321270
epstein-barr virus (ebv)-negative b-lymphoma cell lines for clonal isolation and replication of ebv recombinants.previous experiments have demonstrated that positive selection markers recombined into the epstein-barr virus (ebv) genome enable the isolation of transforming or nontransforming mutant ebv recombinants in ebv-negative b-lymphoma (bl) cell lines (a. marchini, j. i. cohen, and e. kieff, j. virol. 66:3214-3219, 1992; f. wang, a. marchini, and e. kieff, j. virol. 65:1701-1709, 1991). however, virus has been recovered from a bl cell clone (bl41) infected with an ebv recombinant in only one instance ...19921321281
trans-acting requirements for replication of epstein-barr virus ori-lyt.epstein-barr virus (ebv) utilizes a completely different mode of dna replication during the lytic cycle than that employed during latency. the latency origin of replication, ori-p, which functions in the replication of the latent episomal form of the ebv genome, requires only a single virally encoded protein, ebna-1, for its activity. during the lytic cycle, a separate origin, ori-lyt, is utilized. relatively little is known about the trans-acting proteins involved in ori-lyt replication. we est ...19921321285
primary structure of the herpesvirus saimiri genome.this report describes the complete nucleotide sequence of the genome of herpesvirus saimiri, the prototype of gammaherpesvirus subgroup 2 (rhadinoviruses). the unique low-g + c-content dna region has 112,930 bp with an average base composition of 34.5% g + c and is flanked by about 35 noncoding high-g + c-content dna repeats of 1,444 bp (70.8% g + c) in tandem orientation. we identified 76 major open reading frames and a set of seven u-rna genes for a total of 83 potential genes. the genes are c ...19921321287
epstein-barr virus-encoded small rnas (ebers) do not modulate interferon effects in infected lymphocytes.the recent derivation of otherwise isogenic epstein-barr virus (ebv) recombinants carrying or lacking the ebv small rna (eber) genes enabled us to test whether ebers are similar to adenovirus va rnas in modulating interferon (ifn) effects on virus infection. eber-positive and -negative ebv recombinants did not differ in their sensitivity to alpha interferon (ifn-alpha)- or ifn-gamma-mediated inhibition of lymphocyte growth transformation. in addition, ebers did not decrease the inhibitory effect ...19921321292
an epstein-barr virus transformation-associated membrane protein interacts with src family tyrosine latently infected growth-transformed human lymphocytes, epstein-barr virus (ebv) encodes two integral plasma membrane proteins: lmp1, which constitutively induces b-lymphocyte activation and intercellular adhesion, and lmp2a, which associates with lmp1 and is a tyrosine kinase substrate. we now demonstrate that lmp2a associates with src family protein tyrosine kinases, particularly lyn kinase, in nonionic detergent extracts of transfected b lymphoma cells or in extracts of ebv-transformed b l ...19921321296
arcanobacterium haemolyticum sepsis and epstein-barr virus infection. 19921321407
recognition of the epstein-barr virus-encoded nuclear antigens ebna-4 and ebna-6 by hla-a11-restricted cytotoxic t lymphocytes: implications for down-regulation of hla-a11 in burkitt lymphoma.evasion from cytotoxic t-lymphocyte (ctl) surveillance may be an important step in the pathogenesis of epstein-barr virus (ebv)-carrying burkitt lymphoma (bl) as suggested by the consistent down-regulation of all transformation-associated viral antigens, except ebv nuclear antigen 1 (ebna-1), and of certain hla class i alleles in bl biopsies and cell lines that maintain the tumor cell phenotype in vitro. the most common hla class i defect recorded in bl lines is a selective down-regulation of hl ...19921321426
coinfection with multiple strains of the epstein-barr virus in human immunodeficiency virus-associated hairy leukoplakia.epstein-barr virus dna was analyzed from specimens of hairy leukoplakia, an oral lesion that occurs in patients infected with the human immunodeficiency virus. the simultaneous presence of both type 1 and type 2 epstein-barr virus was demonstrated by southern blot analysis and polymerase chain reaction assay. restriction fragment length polymorphisms in the bamhi wyh region and in clones of the ecori c region suggested the presence of multiple strains of type 1 and type 2 viruses. the demonstrat ...19921321443
epstein-barr virus (ebv) genomes and c-myc oncogene in oral burkitt's addition to burkitt's lymphomas, tentative evidence suggests the involvement of epstein-barr virus (ebv) in malignant lymphomas of t-cell origin. the c-myc proto-oncogene is strongly associated with the development of lymphoid neoplasias. in the present study, a series of 38 biopsies of oral lymphomas (29 burkitt's lymphomas, 9 malignant lymphomas of other type) obtained from patients in tanzania were studied using in situ hybridization (ish) and polymerase chain reaction (pcr) for detection ...19921321486
peripheral facial palsy caused by borrelia burgdorferi and viruses in south-western a prospective study from 1983 through 1984, 77 patients (31 men and 46 women with a mean age of 47 +/- 20 years) with peripheral facial palsy of primarily unknown etiology were investigated. only 2 patients with acute otitis media received antibiotics. serology of the patients was investigated on days 1 and 14. igg and igm antibodies against herpes simplex, varicella-zoster and cytomegalovirus were determined by enzyme immunoassay, and against epstein-barr virus by immunofluorescence. in a re ...19921321549
isolation and characterization of mitochondria from human b lymphoblastoid cell lines.mitochondria were isolated from detergent-treated epstein-barr virus-transformed human lymphocytes to examine their potential use in the study of the functional expression of genetic disorders of the respiratory chain. the increase of cytochrome c oxidase activity in the mitochondrial fraction indicated a 6-fold purification of intact mitochondria. polarographic and spectrophotometric studies revealed that the isolated mitochondria were functionally well preserved. furthermore, the isolated mito ...19921321601
detection of epstein-barr virus dna from a lymphoma-like lesion of the uterine cervix.the case of a 60-year-old woman in whom a lymphoma-like lesion of the cervix was found during an episode of silent epstein-barr virus (ebv) infection is presented. fractional curettage was performed because of abnormal endometrial smear. the endocervical curettage specimens were diagnosed as highly suggestive of malignant lymphoma, but microscopic examination of a subsequent hysterectomy specimen revealed a benign lymphoid hyperplasia. those were retrospectively interpreted as a lymphoma-like le ...19921321783
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