the expanding challenge of hiv-associated malignancies. 19921311619
modulation of human ige synthesis by transforming growth factor-beta.exogenous transforming growth factor-beta 2 (tgf-beta 2) markedly inhibits the interleukin 4 (il4)-stimulated synthesis of human ige in three models where the b cell co-stimulation signals are contact dependent. this concerns t cell-dependent ige production by (i) unfractionated peripheral blood mononuclear cells (pmbc) cultured with il4 and (ii) highly purified b cells cocultured with irradiated el4 thymoma cells in the presence of il4 and phorbol myristate acetate, as well as monocyte-dependen ...19921311647
isolation of a clone which induces expression of the gene encoding the human tumor necrosis factor receptor.tumor necrosis factor (tnf) is a cytokine with pleiotropic effects upon cell growth, inflammation and immunologic responsiveness. high-affinity tnf receptors (tnfrs) of 55 and 75 kda are found in many cell types. using an epstein-barr virus (ebv)-based mammalian expression library, we have isolated a clone from human lymphoblastoid transfectants that induces overexpression of the tnfr-encoding gene (tnfr). transfectants overproducing the tnfr were isolated by multiple rounds of sorting on a fluo ...19921311700
a human lymphoma cell line with multiple immunoglobulin rearrangements.the development of a cell culture system efficient in the establishment of lymphoma cell lines has made it possible to dissect basic biological and molecular aspects of lymphoma cells. we have established a lymphoma cell line from a patient with b cell lymphoma. the cell line has a complex karyotype with translocations involving bands 8q24, 14q32, and 18q21. molecular analysis revealed that the myc gene was rearranged; we were unable to demonstrate rearrangement of the bcl-2 gene. evaluation of ...19921311715
a rapid limiting dilution assay for measuring frequencies of alloreactive, interleukin-2-producing t cells in humans.a limiting dilution analysis (lda) has been established which measures the total numbers of alloreactive interleukin-2 (il-2)-secreting t cells in human peripheral blood mononuclear cells (pbmc). a significant advantage over most previous lda is that the assay may be completed in approximately 48 h since an il-2-dependent 'indicator' cell line is used to reduce assay time. results are reproducible and correlate with the degree of hla class ii antigenic disparity between responder and stimulator ...19921311737
n-methyl-n-nitrosourea-induced mutations in human cells. effects of the transcriptional activity of the target this study we addressed the question as to whether the mutagenesis by methylating agents is affected by the transcriptional activity of the damaged gene. an epstein-barr virus (ebv)-derived shuttle vector system was developed where the genetic target for mutation analysis, the bacterial gpt gene, is under the control of an eukaryotic inducible promoter in plasmid pf1-ebv and lacks the eukaryotic promoter in plasmid pf2-ebv. two human cell lines that episomically maintain these shuttle vectors ...19921311769
epstein-barr virus in hodgkin's disease from patients with human immunodeficiency virus infection.the polymerase chain reaction (pcr) technique was used to detect the presence of epstein-barr virus (ebv) dna sequences in hodgkin's disease specimens from 10 patients who were also positive for the human immunodeficiency virus (hiv). eight of 10 specimens were positive for ebv, compared to 23 of 57 hodgkin's disease specimens from patients without hiv infection, suggesting a closer association between epstein-barr virus infection and hodgkin's disease in patients with hiv infection than in the ...19921311840
[serology in infectious mononucleosis in children].28 pediatric patients with signs and symptoms of infections mononucleosis (mi) are presented. we did a serologic study in all of them from two or three serum specimen collected in 2-3 week intervals. we detected igg vca but no antibodies against t. gondii or cmv in all of them. the level of the titers of these antibodies didn't help in the establishment of the phase of the illness. igm vca was present in the serum of 22 patients, with or without heterophil antibodies (ah), meanwhile the remainin ...19921311906
'multifocal hairy leukoplakia'. 19921311940
regulation of the escherichia coli lac operon expressed in human cells.we have investigated the use of various epstein-barr virus (ebv)-based vectors bearing the two components of the escherichia coli lac operator-repressor (laco, laci) complex. our aim was to develop a model system of gene expression by looking at the transcription of the bacterial beta-galactosidase coding gene (lacz) in 293 human embryonic kidney cells. several vectors have been built carrying different promoters upstream of the laci and lacz genes and in which natural or synthetic operator sequ ...19921311956
epstein-barr virus and hiv play no direct role in persistent generalized lymphadenopathy syndrome.persistent generalized lymphadenopathy (pgl) and polyclonal b cell activation are features of infection with hiv. epstein-barr virus (ebv) and hiv are known to activate b cells in vitro, but whether they are important b cell activators in patients infected with hiv is less clear. in this study, lymph node tissue was obtained from 10 patients with pgl and assessed for evidence of ebv and hiv gene sequences. dna was extracted and specific viral gene sequences identified using the polymerase chain ...19921311993
effect of tgf-beta 1 on the ebv-induced transformation of human lymphocyte cultures.we tested the effect of tgf-beta 1 on the ebv-induced activation and immortalization of human b lymphocytes. in lymphocyte cultures of ebv-seropositive individuals, t cells can inhibit the ebv-induced transformation of b cells. we found that in the presence of tgf-beta 1 the transformation was more efficient, due to the inhibition of this function. tgf-beta 1 inhibited the ebv-induced proliferation of purified b-cell cultures. its effect was strongest when added at the beginning of the culture. ...19921312065
expression of type iv collagenase correlates with the invasion of human lymphoblastoid cell lines and pathogenesis in scid in vitro model, called the membrane invasion culture system (mics), was used to study the invasive potential of an epstein-barr virus (ebv) positive lymphoblastoid cell line (lcl), an ebv-negative burkitt lymphoma (bl) cell line of american origin and an ebv-positive bl of african origin. mics measured the ability of these cell lines to invade reconstituted basement membrane-coated filters, which correlated with their tumorigenic and metastatic capabilities in a scid mouse model. furthermore, ...19921312223
[infection by human herpesvirus type 6: epidemiology, immunopathology and clinical implications].the human herpesvirus type 6 has been discovered recently and is the object of numerous investigations. even though, its morphology is very close to the cytomegalovirus, its epidemiologic, immunopathologic and clinic characteristics are similar to the epstein-barr virus. like the latter, hhv-6 persists latent in the host during all his live, frequently relapsed and is ubiquitous. exanthema subitum in children and mononucleosis-like syndrome in adults have been attributed to acute hhv-6 infection ...19921312248
dna binding activity is required for ebna 1-dependent transcriptional activation and dna replication.epstein-barr virus nuclear antigen 1 (ebna 1) has been shown to be a sequence-specific dna binding protein that is required for the replication of episomal elements carrying the viral origin of dna replication, orip, as well as for the activation of a specific transcriptional enhancer. we have constructed and analyzed a series of deletion and nonsense mutants in a cloned copy of the ebna 1 gene and have tested mutant peptides for the ability (a) to bind to a synthetic oligonucleotide containing ...19921312270
epstein-barr virus in b lymphocytes: viral gene expression and function in latency. 19921312289
a nuclear matrix attachment region organizes the epstein-barr viral plasmid in raji cells into a single dna domain.the extrachromosomal epstein-barr virus (ebv) plasmid in the burkitt lymphoma cell line, raji, is stably associated with the nuclear matrix. this association is effected by a nuclear matrix attachment region (mar) located in the bamhi c fragment of the viral genome; no other region of ebv dna was found to be attached to the nuclear matrix with high affinity. the mar was mapped to 5.2 kbp of dna, greater than 80% of which is found on the nuclear matrix in unsynchronized cells expressing only vira ...19921312463
immunoglobulin production in severe combined immunodeficient (scid) mice reconstituted with human peripheral blood mononuclear cells.immunodeficient c.b-17 scid/scid (scid) mice were reconstituted with human peripheral blood mononuclear cells and analyzed for humoral immunity. the majority of adoptively transferred animals had serum levels of 1-4 mg/ml of human igg 8-12 weeks after i.p. reconstitution with 20 x 10(6) pbmc, whereas the igm and especially iga concentrations were considerably lower. the half-lives of human igg, igm, and iga in scid mice were 12 days, 36, and 23 hours, respectively. furthermore, iga was rapidly s ...19921312475
bhrf1 of epstein-barr virus, which is homologous to human proto-oncogene bcl2, is not essential for transformation of b cells or for virus replication in vitro.the epstein-barr virus (ebv) genome contains an open reading frame, bhrf1, that encodes a presumptive membrane protein with sequence similarity to the proto-oncogene bcl2, which is linked to human b-cell follicular lymphoma. potential roles for bhrf1 in ebv's ability to growth transform human b cells and to replicate in b cells in culture were investigated by generating ebv mutants that lack most of the open reading frame. this was accomplished by recombination of plasmids carrying mutations in ...19921312610
viruses and hodgkin's disease. 19921312656
epstein-barr virus infection and replication in a human epithelial cell system.epstein-barr virus, a human herpesvirus with oncogenic potential, infects two target tissues in vivo: b lymphocytes, where the infection is largely non-productive, and stratified squamous epithelium in which virus replication occurs. the interaction with b cells, initiated through virus binding to the b-cell surface molecule cr2 (ref. 4), has been studied in vitro and the virus 'latent' genes associated with b-cell growth transformation defined. by comparison, viral infection of epithelium remai ...19921312681
significance of oral hairy leukoplakia.since the original description of oral hairy leukoplakia among homosexual men in san francisco in 1984, this white lesion of the tongue has been seen in the mouths of persons infected with the human immunodeficiency virus (hiv) worldwide. its presence in hiv-positive persons usually but not always indicates fairly rapid progression to acquired immunodeficiency syndrome in the absence of antiretroviral therapy. although the lesion appears to be common in hiv-positive persons, it is also, albeit r ...19921312689
viral infections of the head and neck among hiv-seropositive patients.many viruses cause opportunistic infections in hiv-positive patients. those that cause oral lesions include herpes simplex, varicella zoster, epstein-barr virus, cytomegalovirus, and papillomavirus. importantly, many of the herpes-group viruses are able to augment immunosuppression and some actually transactivate hiv replication-inducing genetic sequences. this article reviews the role of viral agents in the activation of hiv replication and details the features of the reported oral lesions that ...19921312690
immunoglobulin a-induced shift of epstein-barr virus tissue tropism.increased immunoglobulin a (iga) antibodies to the epstein-barr virus (ebv) appear months to years before the clinical onset of nasopharyngeal carcinoma and define populations at high risk for this ebv-associated epithelial cancer common in south china. in the human ht-29 epithelial cell line, polymeric iga (piga) specific for ebv promoted infection of the otherwise refractory epithelial cells. when bound to piga, ebv entered epithelial cells through secretory component-mediated iga transport bu ...19921312750
lupus anti-dna antibodies bearing the 8.12 idiotype appear to be somatically mutated.anti-dna antibodies in systemic lupus erythematosus (sle) sera were analyzed using an antiidiotype designated 8.12 which recognizes a determinant on lambda light chains highly expressed in sle sera. eight of ten normal individuals had peripheral blood lymphocytes which produced high-titered 8.12-positive antibodies, following transformation with epstein barr virus, implying that the 8.12-reactive sequence originates in the germline gene (glg). of 58 sle sera, 32 contained elevated titers of 8.12 ...19921313042
detection of epstein-barr virus genome in natural-killer-like cell line, cells, originally reported as a natural-killer-like (nk-like) lymphoid cell line, were investigated for epstein-barr virus (ebv) genome, gene rearrangement for t-cell receptor (tcr), phenotype and function. the yt cells of the original batch (yt-0) and two subclones (yt2c2 and ytc3) expressed ebv-associated nuclear antigen, and the bamhi-digested dna showed the 3.4 kb hybridizing band with the bamhiw probe of ebv dna in southern blot analysis. when tested with latent-infection membrane protei ...19921313126
establishment of a new human b-cell line (bonna-12) with trisomy 9 and trisomy 12 chromosomal abnormality. 19921313127
a simple and rapid technique to process formalin-fixed, paraffin-embedded tissues for the detection of viruses by the polymerase chain reaction.the use of chelating resin in a simple, rapid and efficient pre-treatment protocol to process formalin-fixed, paraffin-embedded specimens for the polymerase chain reaction (pcr) is described and compared to other pre-treatment techniques. with this modified pcr protocol, a variety of human autopsy and biopsy specimens were investigated for presence of dna of human papilomaviruses, cytomegalovirus or epstein-barr virus. these viruses were detected in a productive or non-/low-productive state. amp ...19921313196
detection of epstein-barr virus in lymphocytic interstitial pneumonia by in situ hybridization.the involvement of epstein-barr virus (ebv) in the pathogenesis of lymphocytic interstitial pneumonia (lip) was investigated using an in situ hybridization technique. archival lung tissue samples from 14 patients (six men and eight women with a mean age of 58 +/- 3 yr) in whom a diagnosis of lip had been previously established were retrospectively examined and compared with samples from 10 patients (six men and four women with a mean age of 58 +/- 3 yr) with idiopathic pulmonary fibrosis (ipf) w ...19921313215
involvement of major histocompatibility complex class ii antigen in epstein-barr virus-mediated b cell proliferation.five mhc class ii monoclonal antibodies costimulated proliferation of cord blood leukocytes with epstein-barr virus. these agonistic antibodies were of different isotypes, but all of them were either specific for or cross-reacting with hla-dr. the other mhc class ii antibodies, including three that were specific for hla-dq and one that was specific for hla-dp and also those that were specific for mhc class i or leukocyte common antigen, were not costimulatory. the agonistic effect of different m ...19921313338
detection of epstein-barr virus dna in a japanese case of lymphoepithelioma-like thymic carcinoma.epstein-barr virus dna was detected in a case of lymphoepithelioma-like thymic carcinoma. a homogeneous terminal structure of the viral dna was demonstrated in this case, indicating the presence of the viral genome in clonally expanded tumor cells. since all of 26 other thymic epithelial tumors (eight non-invasive, 13 invasive thymomas and four non-lymphoepithelioma-like thymic carcinomas) in japanese were negative by polymerase chain reaction, it is suggested that lymphoepithelioma-like thymic ...19921313408
epstein-barr virus and hodgkin's disease. 19921313457
immunocytochemical detection of herpes viruses in oral smears of hiv-infected patients.cytologic smears (cs) were taken from the lateral border of the tongue of hiv-seropositive patients (hiv+) (n = 39) and of seronegative controls (hiv-) (n = 19) and examined by immunocytochemistry (apaap) and in situ hybridization (ish) (biotinylated dna probes) for the presence of viral antigens/dna of ebv and cmv. while none of the hiv controls showed positive results for ebv antigen, 61% (apaap) resp. 79% (ish) of oral epithelial cells in the group of hiv+ patients were ebv-positive. while al ...19921313501
igg antibodies to human herpesvirus-6 (hhv-6) in italian people.sera from a sample of healthy italian people were tested in an indirect immunofluorescence assay (ifa) for reactivity to a human herpesvirus-6 (hhv-6) strain called cv, isolated from a baby with exanthem subitum (portolani et al., 1990). seropositivity values of 83.78%, 92.68% and 63.64% were found in subjects aged 3 months-6 years, 6-18 years and over 18 years respectively. sera from cordal blood, sera from subjects with evidence of active infection by cytomegalovirus (cmv) or by epstein-barr v ...19921313531
frequency of ebv dna detection in sjögren's syndrome. 19921313639
interleukin-5 is an autocrine growth factor for epstein-barr virus-transformed b lymphocytes.because of the recent finding that interleukin-5 (il-5) is produced by epstein-barr virus-transformed b lymphocytes (ebv-b cells), we performed studies to ascertain whether ebv-b cells might use il-5 by an autocrine mechanism. ebv-b cells known to be il-5 producers were capable of responding to addition of exogenous il-5 by dose-related augmented proliferation. the addition of a neutralizing anti-il-5 antibody reduced these effects and also dose-dependently inhibited proliferation and reduced vi ...19921313713
retroviral expression of recombinant p47phox protein by epstein-barr virus-transformed b lymphocytes from a patient with autosomal chronic granulomatous disease.patients with chronic granulomatous disease (cgd) have recurrent infections resulting from a failure of phagocytic cells to produce superoxide. one third of cgd patients have an autosomal gene defect resulting in absence of p47phox protein, a cytoplasmic component critical to superoxide production by phagocytic cells. cdna encoding p47phox has been cloned and recombinant p47phox (rp47phox) restores superoxide-generating activity to a cell-free assay containing cell membranes and cytosol from p47 ...19921313715
detection of epstein-barr virus in lymphoid tissues of patients with infectious mononucleosis by in situ hybridization.although the immunological response during infectious mononucleosis (imn) has been studied in detail, little is known about the spread of epstein-barr virus (ebv) in lymphoid organs or the topographical distribution of the infected cells. in this study, ebv was detected in 11 lymph nodes, 4 tonsils, and 1 spleen of 16 patients with imn. the predominant cell type positive for the ebv genome was identified as small lymphocytes localized chiefly within typical t areas, preferentially in perifollicu ...19921313863
hepatitis b virus (hbv)-specific cytotoxic t-cell response in humans: production of target cells by stable expression of hbv-encoded proteins in immortalized human b-cell analyze the hepatitis b virus (hbv)-specific cytotoxic t-cell (ctl) response during acute and chronic viral hepatitis, target cells that express hbv-encoded antigens in the context of the appropriate hla restriction element must be available for each subject studied. since hbv is not infectious for human cells in vitro, such target cells must be produced by dna-mediated gene transfer into cultured human primary cells or cell lines. for this purpose, we have developed a panel of epstein-barr v ...19921313893
epstein-barr virus latent gene transcription in nasopharyngeal carcinoma cells: coexpression of ebna1, lmp1, and lmp2 transcripts.epstein-barr virus (ebv) genome-positive nasopharyngeal carcinomas (npcs) regularly express the virus-coded nuclear antigen ebna1, but not other ebnas, and a subset of tumors also appear to be latent membrane protein lmp1 positive; the status of npcs with respect to a second virus-coded latent membrane protein lmp2 is unknown. in the present work the ebv-npc cell interaction has been analyzed at the rna level with reverse transcription and polymerase chain reaction-based amplification to detect ...19921313894
regulation of the epstein-barr virus dna polymerase gene.the gene (pol) encoding the epstein-barr virus (ebv) dna polymerase is a member of the "early" class of viral genes which are expressed shortly after activation of latent virus infection. first, mrna from the ebv-producing cell line, b95-8, treated with 12-o-tetradecanoylphorbol-13-acetate and sodium butyrate to induce lytic replication and expression of this gene was analyzed. northern (rna) analysis revealed a message of 3.7 kb found only in induced cells. 5' mapping of pol mrna by s1 nuclease ...19921313904
identification of critical cis elements involved in mediating epstein-barr virus nuclear antigen 2-dependent activity of an enhancer located upstream of the viral bamhi c promoter.the six genes encoding the epstein-barr virus nuclear antigens (ebnas) are transcribed from one of two promoters, bamhi c promoter (cp) or bamhi w promoter (wp), located near the left end of the viral genome. during the establishment of viral latency in b lymphocytes, wp is used exclusively before a switch to cp usage. we and others have previously identified an enhancer in the region upstream of cp which requires ebna 2 for activity (m. woisetschlaeger, x. w. jin, c. n. yandava, l. a. furmanski ...19921313905
use of second-site homologous recombination to demonstrate that epstein-barr virus nuclear protein 3b is not important for lymphocyte infection or growth transformation in vitro.recombinant epstein-barr viruses with a stop codon inserted into the nuclear protein 3b (ebna 3b) open reading frame were generated by second-site homologous recombination. these mutant viruses infected and growth transformed primary b lymphocytes, resulting in the establishment of lymphoblastoid cell lines (lcls). polymerase chain reaction analysis and southern hybridizations with infected cell dna demonstrated the presence of the mutant ebna 3b and the absence of wild-type ebna 3b. immunoblot ...19921313908
a selectable marker allows investigation of a nontransforming epstein-barr virus mutant.the derivation of specifically mutated epstein-barr virus (ebv) recombinants is dependent on strategies to identify, enumerate, and clone infected b lymphocytes. in recent experiments, ebv recombinants containing a positive selection marker were identified and cloned in b-lymphoma (bl) cells infected and then plated under selective conditions (f. wang, a. marchini, and e. kieff, j. virol. 65:1701-1709, 1991). we now use bl cells, for the first time, as hosts for assaying and cloning otherwise is ...19921313925
consistent transcription of the epstein-barr virus lmp2 gene in nasopharyngeal carcinoma.two species of the epstein-barr virus-encoded latent membrane protein 2, lmp2a and lmp2b, are generated by alternative splicing, each species having a distinct first exon. lmp2 transcription in undifferentiated nasopharyngeal carcinoma (npc), which is consistently associated with the epstein-barr virus, was investigated. fifteen npc specimens were analyzed by northern (rna) blot and rna-based polymerase chain reaction; the lmp2a transcript was present in all specimens except one. in some specime ...19921313931
[severe leukopenia and thrombocytopenia and transient acquired hypogammaglobulinemia in a patient with infectious mononucleosis].a 30-year-old male with infectious mononucleosis by the epstein-barr virus who presented severe neutropenia and thrombocytopenia and type igg acquired transitory hypogammaglobulinemia as complications during the acute period of the disease is presented. although the etiopathogenesis of these phenomena is usually associated with an autoimmune basis, the antiplatelet and antileukocyte antibodies were negative. a bibliographic revision of the hematologic alterations of this disease was carried out ...19921313937
nasopharyngeal carcinoma. 19921313945
presence of epstein-barr virus in hodgkin's disease is not exclusive to reed-sternberg cells.thirty-three cases of hodgkin's disease (hd) have been studied for the presence of epstein-barr virus (ebv) using a novel nonisotopic in situ hybridization procedure, based on the detection of epstein-barr encoded rnas with oligonucleotide probes. an intense and morphologically distinct nuclear staining, sparing the nucleolus was seen in a total of 12 cases (36%). in six of these cases, the signal was located to the hodgkin and reed-sternberg cells (hr-s); in the other six positive cases, the si ...19921314022
epstein-barr virus-associated gastric adenocarcinoma.the epstein-barr virus (ebv) has been detected in certain types of lymphoma and some epithelial neoplasms including nasopharyngeal lymphoepithelioma, and rare lymphoepithelioma-like carcinomas occurring in a variety of organs including, most recently, the stomach. the authors investigated the possibility that ebv may be present not only in the rare gastric cancers that resemble nasopharyngeal lymphoepithelioma, but also in typical gastric adenocarcinoma. ebv sequences were detected in 22 of 138 ...19921314023
expression of epstein-barr virus genes and of lymphocyte activation molecules in undifferentiated nasopharyngeal carcinomas.previous studies investigating the role of epstein-barr virus (ebv) in undifferentiated nasopharyngeal carcinoma (npc) have been performed on extracts from biopsies. the authors analyzed expression and localization of viral gene products in 18 undifferentiated npcs at the cellular level using immunohistology and in situ hybridization. all cases were ebv-positive. the small nuclear ebv-encoded rnas, ebers, were regularly expressed whereas the latent membrane protein, lmp1, and ebv was detectable ...19921314028
clonal epstein-barr virus genome in t-cell-rich lymphomas of b or probable b lineage.seventeen nodal lymphomas (originally diagnosed as t-cell lymphomas based on histological features and immunohistochemical staining results) were studied for the presence of epstein-barr virus (ebv) genome, and the results correlated with immunoglobulin and t-cell receptor gene rearrangement analyses performed on the same tissue samples. all four ebv positive cases had clonal rearrangement of the joining region of the immunoglobulin heavy chain (igjh) gene without clonal t-cell receptor beta-cha ...19921314029
virus-associated rnas (va-i and va-ii). an efficient target for the detection of adenovirus infections by in situ hybridization.the identification of adenovirus in tissue can be difficult. in situ hybridization for adenovirus nucleic acids may aid in the demonstration of adenovirus infections. to develop a probe against adenovirus, a 978 bp fragment of dna containing the va-i, va-ii, and a portion of the l-1 regions of the adenovirus type 2 genome was cloned into the sk+ vector. these regions were selected because they are generally conserved among adenoviruses and are abundantly transcribed during the lytic cycle. sense ...19921314030
monoclonal antibodies for the identification of herpesvirus simiae (b virus).to differentiate between b virus and hsv isolates from monkeys and man monoclonal antibodies (mabs) were produced to herpesvirus simiae (b virus) and herpes simplex type 1 and 2 (hsv-1 and hsv-2). mabs were tested by indirect immunofluorescence (ifat) for reactivity against herpesviruses from asiatic monkeys (b virus), african monkeys (sa 8 virus), and man (hsv-1, hsv-2, varicella-zoster virus, cytomegalovirus, and epstein-barr virus). mabs could be divided into groups a-e displaying specific re ...19921314049
epstein-barr viral dna in acute large granular lymphocyte (natural killer) leukemic cells.serologic studies in a male caucasian presenting with an acute hepatitis-like illness, associated with an increase in peripheral blood large granular lymphocytes (lgls), suggested a chronic or reactived epstein-barr virus (ebv) infection. the lgl were shown to have a natural killer (nk) cell, cd3- cd16- cd56+ cd57- phenotype and mediated strong nonspecific major histocompatability complex-unrestricted (nk) cytotoxic activity. a progressive increase in the peripheral blood lgl count was associate ...19921314113
host cell reactivation of cisplatin-damaged plasmid dna in human non-t leukocyte cell reactivation of cisplatin-damaged prsvcat was assessed in three b cell lines (skw 6.4, wil2-ns, rpmi 1788), the monocytic cell line thp-1, and promyelocytic hl-60 cells. ic50 values following a 3-day exposure of the five leukocyte cell lines to cisplatin ranged from 0.45 to 1.92 microm. transfer of prsvcat into all cell lines was effected by electroporation and the resultant cat activity was measured 24 h later by a rapid single vial cat assay. cat activity corresponding to an average ...19921314131
antibodies to human herpes virus-6 and clinical course in patients with hodgkin's disease.serial serum samples from 37 patients with hodgkin's disease (hd) and 39 healthy controls were studied for antibodies to human herpes virus-6 (hhv-6) using elisa and indirect immunofluorescent antibody (ifa) tests and to the epstein-barr virus (ebv) using a radio-complement fixation assay. antibodies to hhv-6 in the pre-treatment sera from the hd patients were not significantly different from those of controls, but significant changes in titers related to clinical course were noted among the hd ...19921314231
high titers of epstein-barr virus antibodies in adult patients with lymphocytic interstitial pneumonitis associated with aids. 19921314403
analysis of nucleotide sequence of the rightmost 43 kbp of herpesvirus saimiri (hvs) l-dna: general conservation of genetic organization between hvs and epstein-barr virus.we present an analysis of 43,658 bp of contiguous nucleotide sequence comprising the right terminal region (conventional orientation) of the unique protein-coding component (l-dna) of the herpesvirus saimiri (hvs) genome. within this region lie the genes encoding the 160-kda virion protein, which is homologous to the 140-kda membrane antigen of epstein-barr virus (ebv), thymidylate synthase (ts), and the immediate-early (ie) 52-kda protein which is homologous to the ebv bmlf1 product. the 160-kd ...19921314457
[viral etiology of sjögren's syndrome]. 19921314679
globic: a very fast microcomputer program for fingerprinting, characterization and comparison of long nucleotide sequences.this paper describes the program globic, which compares, characterizes and fingerprints even 0.1 mbase sequences in a few minutes with the aid of an ibm-at microcomputer. instead of the nucleotide sequences themselves, globic compares the local nucleotide or short oligonucleotide compositions. globic presents two-dimensional maps of contour lines depicting the similarity of two different sequences, a sequence compared to itself, to its complementary sequence or to a random sequence. a vocabulary ...19921314685
heterogeneity among epstein-barr virus-seropositive donors in the generation of immunoblastic b-cell lymphomas in scid mice receiving human peripheral blood leukocyte grafts.epstein-barr virus (ebv) is associated with b-cell malignancy in immunosuppressed humans and scid mice receiving human peripheral blood leukocyte grafts (hu-pbl-scid). we have further characterized the process of lymphoma development in hu-pbl-scid mice. we report that ebv-seropositive donors differ markedly in the capacity of their pbl to give rise to immunoblastic lymphomas in scid mice; some donors (high incidence) generated tumors rapidly in all hu-pbl-scid mice, other donors (intermediate-l ...19921314693
[histiocyte activation syndrome associated with ebv in children].vahs (virus associated hemophagocytic syndrome) is a severe clinical and biological condition associated with certain menacing viral infections. it has been observed in patients with manifest immunodeficiency but also in previously apparently healthy children. epstein-barr virus (ebv) infection, although it is sometimes unrecognized and insidious, is one of the etiologies to be considered. differential diagnosis with other causes of macrophage activation is a delicate task in pediatrics. better ...19921314725
lymphoid pneumonitis in 50 adult patients infected with the human immunodeficiency virus: lymphocytic interstitial pneumonitis versus nonspecific interstitial pneumonitis.lymphocytic interstitial pneumonitis (lip) and nonspecific interstitial pneumonitis (nip) are pulmonary complications of human immunodeficiency virus (hiv) infection that occur in the absence of a detectable opportunistic infection or neoplasm. we reviewed lung biopsy specimens from 50 adult hiv-infected patients, of whom four had lip and 46 had nip. the majority (47 of 50) of specimens from patients with nip showed mild chronic interstitial pneumonitis (cip/nip), with three showing features of ...19921314778
epstein-barr virus serology in the chronic fatigue syndrome.the antibody profiles against epstein-barr virus were studied in 136 patients presenting with chronic fatigue syndromes. these profiles were compared with a panel of sera from blood donors. the patients exhibited higher titres in a combined assay for antibodies to the restricted (r) and diffuse (d) components of the early antigen complex than controls (p less than 0.001) but titres against these antigens were not useful on an individual patient basis. the patients who displayed elevated titres o ...19921314860
subclinical epstein-barr virus infection of both the male and female genital tract--indication for sexual transmission.epstein-barr virus (ebv) can infect b lymphocytes as well as epithelial cells of the oral cavity. recently, infection of epithelial cells of the inflamed uterine cervix has been demonstrated, and ebv-dna has been detected in urethral discharge of men suffering from genital infection. we investigated whether ebv can be found in the genital tract of both sexes independently from inflammatory disease states. genital specimens of men and women of a sexually transmitted diseases outpatient clinic aft ...19921314867
[acute heart insufficiency in an 8-month-old infant presenting with hypocalcemia and epstein-barr virus infection: acute myocarditis? or primary hypokinetic dilated cardiomyopathy?].an eight-month-old was admitted for acute congestive heart failure with fever. the respective parts played by hypocalcemia (due to vitamin-d deficiency rickets) and acute epstein-barr virus infection are discussed. hypocalcemia was sufficiently marked to induce heart failure per se but replenishment of calcium stores was followed by only partial improvement in cardiac manifestations. initial management was difficult because of the risks associated with concomitant administration of calcium and d ...19921315131
facial palsy and infection: the unfolding story.while it is now universally accepted that ramsay hunt syndrome is caused by varicella-zoster virus, bell's palsy continues to be labeled "idiopathic." we review the literature associating bell's palsy with various infectious agents as well as with kawasaki disease, a condition in which an infectious etiology is suspected. good evidence--mostly serological--exists for an etiologic role for the herpes group of viruses, mumps virus, and rubella virus. in addition, recent evidence has focused on bel ...19921315161
detection of an idiotope on a human monoclonal autoantibody by monoclonal anti-idiotypic antibody and its relationship to epstein-barr virus-induced autoimmunity.we have recently described a human igm monoclonal antibody (mab), reactive with both self antigens, i.e., cytoskeleton filaments and smooth muscle, and epstein-barr virus (ebv)-induced nuclear antigen (ebna), produced by ebv-transformed b lymphocytes isolated from a patient with infectious mononucleosis (im). in order to achieve higher antibody secretion in culture supernatant, the mab-producer cells were fused with ouabain-resistant mouse myeloma cells and a stable human-mouse heterohybrid, cod ...19921315162
production of human monoclonal antibodies: potential therapeutic use in gut-derived infectious-toxic shock syndromes. 19921315241
comparison of an ebv transformed cell line and an ebv hybridoma cell line producing the same human anti-hbs monoclonal antibody.a stable human-mouse heterohybridoma secreting human anti-hbs monoclonal antibody in continuous culture for 12 months was generated. it grew faster than the parent ebv transformed lymphoblastoid cell line (lcl) but produced the same level of specific antibody. the lcl was positive for the epstein-barr virus nuclear antigen (ebna), human cd 23 and contained a diploid number of human chromosomes. the heterohybridoma was negative for ebna, cd 23 and mouse ly-1 mouse, despite retaining a full comple ...19921315325
epstein-barr virus detection in neck metastases by polymerase chain reaction.cervical nodal metastasis from occult carcinomas represents a diagnostic challenge. this is a common presentation of undifferentiated nasopharyngeal carcinoma (unpc), but metastatic carcinomas from other sites must be considered. unpc has the distinguishing feature of a close association with epstein-barr virus (ebv). since the polymerase chain reaction (pcr) can detect ebv in archival tissues, it offers significant advantages over previous methods for the detection of viral genomes. its extreme ...19921315410
cloning muscle isoforms of neural cell adhesion molecule using an episomal shuttle vector.three distinct transcripts encoding two phosphatidylinositol (pi) linked isoforms of the neural cell adhesion molecule (ncam) are induced during the differentiation of c2c12 myoblasts into myotubes. corresponding ncam clones were isolated from a mouse muscle cdna library made in an epstein-barr virus shuttle vector that replicates extrachromosomally in human cells. following transfection with the library, human cells expressing mouse ncam were enriched using the fluorescence-activated cell sorte ...19921315456
interaction between epstein-barr virus and a t cell line (hsb-2) via a receptor phenotypically distinct from complement receptor type 2.epstein-barr virus (ebv), the causative agent of mononucleosis and several human cancers, infects cells via complement receptor type 2 (cr2, cd21) which also serves as the receptor for the third complement component, c3. expression of this receptor is restricted to b lymphocytes, immature thymocytes, and certain epithelial cells. in the present investigation; we describe the presence of a seemingly novel ebv receptor which is phenotypically distinct from cr2. among various leukemic t cells studi ...19921315687
[epstein-barr virus infection--a lympho- and epitheliotropic infection].epstein-barr virus (ebv) has long been thought to be primarily a b-lymphotropic virus. this tropism becomes obvious in the association of the virus with diseases that become manifest in lymphoproliferative conditions, such as acute infectious mononucleosis or endemic burkitt's lymphoma. in the course of mononucleosis, however, viraemia cannot be detected and b-lymphocytes infected with ebv in vitro produce only small amounts of the virus. in contrast, recent studies document that ebv replicates ...19921315723
epstein-barr virus infection and von willebrand disease. 19921315855
chemotherapy for lymphoid malignancy in immunosuppressed patients. 19921315856
presence of the diffuse early antigen of epstein-barr virus in lymphomas and lymphoproliferative disorders.the authors recently demonstrated that 40% of epstein-barr virus (ebv) associated lymphoproliferative lesions contained lytic as well as latent ebv dna. to examine more fully the replicative state of ebv in these disorders, the authors studied protein extracts of ebv-associated lymphoid lesions from 13 patients, most of whom were immunosuppressed, for expression of the diffuse early antigen (ea-d) of ebv, by immunoblotting techniques. the reagent used was a mouse monoclonal antibody. seven of th ...19921316087
acute progressive epstein-barr virus infections.a few epstein-barr virus (ebv) infections result in progressive, potentially fatal disease. nearly all of these progressive ebv infections occur in individuals known or suspected to be immunodeficient. diagnosis and treatment are difficult.19921316095
[the clinical usefulness of the serological study of igg antibodies and iga against the antigens associated with the epstein-barr virus in patients with nasopharyngeal carcinoma].the relationship between nasopharyngeal carcinoma (npc) and epstein-barr virus (ebv) has been already well established. titulation of the antibodies igg and iga against ebv-related antigens has been proved reliable but always suitable having tested and age-matched control population. we present here the preliminary results from the 14 patients bearing npc and 12 patients with other carcinoma of the head and neck and healthy donors.19921316136
epstein-barr virus based expression vectors. 19921316260
serum levels of lymphokines and soluble cellular receptors in primary epstein-barr virus infection and in patients with chronic fatigue syndrome.the immunopathology in primary epstein-barr virus (ebv) infections and in chronic fatigue syndrome was studied by examining serum levels of interleukins (il) and of soluble t cell receptors in serum samples. serum samples were from patients during and 6 months after primary ebv-induced infectious mononucleosis and from patients with chronic fatigue syndrome and serologic evidence of ebv reactivation. markers for t lymphocyte activation (soluble il-2 and cd8) and for monocyte activation (neopteri ...19921316417
oral signs and symptoms in 160 greek hiv-infected hundred and sixty hiv-infected greek patients were prospectively examined and the oral signs and symptoms were recorded. at the time of oral examination, 76 patients were asymptomatic seropositive, 47 were in arc stage and 37 had aids. one or more oral findings were recorded in 90.6% of the patients while a total of 33 different lesions were observed. the more common oral lesions (highly suspicious) were candidiasis (61%), hairy leukoplakia (24%), periodontitis (19%), necrotizing gingivitis ...19921316436
binding of ebna-1 to dna creates a protease-resistant domain that encompasses the dna recognition and dimerization functions.the epstein-barr virus nuclear antigen ebna-1 is essential for replication of the viral dna during latency. ebna-1 binds as a dimer to palindromic recognition sequences within the plasmid origin of replication, ori-p. in this study, proteinase k susceptibility has been used to further characterize the dna-binding domain of ebna-1. limited protease digestion of ebna-1 (amino acids 408 to 641) generated a smaller dna-binding species that had a degree of inherent protease resistance. when ebna-1 wa ...19921316452
epstein-barr virus enters b cells and epithelial cells by different routes.epstein-barr virus (ebv) infects two cell types, b lymphocytes and epithelial cells. electron microscopic studies have shown that the virus fuses with the lymphoblastoid cell line raji but is endocytosed into thin-walled non-clathrin-coated vesicles in normal b cells before fusion takes place. to compare early interactions of ebv with epithelial cells and b cells, a fluorescence dequenching assay of fusion was employed, using virus labeled either with the ph-insensitive probe octadecyl rhodamine ...19921316456
epstein-barr virus latent gene expression in uncultured peripheral blood this study of epstein-barr virus (ebv) latency, the polymerase chain reaction was used in modified form for amplification and detection of viral mrna sequences in peripheral blood lymphocytes from healthy seropositive adults. six known promoters for latent gene expression and eight known gene products were identified in in vitro-immortalized lymphocytes and in the cell lines established spontaneously from seropositive adults. we examined whether mrna expression in uncultured b cells from four ...19921316478
lymphoproliferative disorder masquerading as rejection in liver transplant recipients--an early aggressive tumor with atypical presentation. 19921316652
infection of the htlv-i-harbouring t-lymphoblastoid line mt-2 by epstein-barr virus.epstein-barr virus (ebv), a ubiquitous human b-lymphotropic virus, is associated with certain lymphoproliferative diseases of t-cell lineage. to understand the mechanism by which ebv infects t cells, we have tested the susceptibility of various human t-cell lines to the virus. we report here that the htlv-i-harbouring t-lymphoblastoid line mt-2 carries a high level of cd21/ebv receptors on their surface, adsorbs fluorescein isothiocyanate-labeled ebv, and synthesizes virus latent antigens (ebna- ...19921316683
phosphorylation of the epstein-barr virus bzlf1 immediate-early gene product zebra.expression of the epstein-barr virus (ebv) bzlf1 gene product zebra is a first step in the cascade of the virus-productive cycle. zebra protein was detected by immunoblotting as a single band at 38 kda in akata cells after crosslinkage of membrane immunoglobulin g (igg) with anti-igg antibody. immunoprecipitation of [32p]phosphate-labeled, anti-igg-stimulated akata cells with anti-zebra antibody showed that zebra was phosphorylated. phosphoamino acid analysis demonstrated phosphorylation of seri ...19921316687
coincident kaposi sarcoma and t-cell lymphoma in a patient with the wiskott-aldrich syndrome.a 24 year old male with a history of eczema, recurrent mild infections, and thrombocytopenia consistent with the wiskott-aldrich syndrome (was) presented with a mediastinal mass, generalized lymphadenopathy, splenomegaly, and severe thrombocytopenia. studies of immune function including immunoglobulin levels and t-cell subsets were normal. furthermore, his t lymphocytes proliferated normally in response to phytohemagglutinin, concanavalin a, and the combination of neuraminidase/galactose oxidase ...19921316718
patterns of epstein-barr virus infection in non-neoplastic lymphoid tissue.taking advantage of the abundant expression of the small epstein-barr virus (ebv)-encoded rnas (ebers) in latently infected cells, we have analyzed 72 normal and hyperplastic lymph nodes and three tonsils of acute infectious mononucleosis (im) for the presence and distribution of ebv+ cells using eber-specific in situ hybridization, in some cases combined with immunohistologic demonstration of cell type-characteristic antigens. in im, large numbers of ebv+ lymphoid b blasts were detectable in ex ...19921316783
bone marrow transplant recipients have defective mhc-unrestricted cytotoxic responses against cytomegalovirus in comparison with epstein-barr virus: the importance of target cell expression of lymphocyte function-associated antigen 1 (lfa1).cytomegalovirus (cmv) remains the most common single infective cause of death following allogeneic bone marrow transplantation (bmt) from major histocompatibility complex (mhc)-identical siblings, whereas epstein-barr virus (ebv)-related disease is infrequent. we show here that mhc-unrestricted cytotoxic effector cells in the peripheral blood of bmt recipients are highly effective at killing ebv-infected target cells, but are inactive against cmv-infected target cells. differential cytotoxicity ...19921316791
viruses as possible etiological factors in human genital cancer.the antibodies against herpes virus were detected in 34 out of 60 (55%) patients with gynecological malignancies. immunofluorescence revealed herpes virus antigens in 40 (66.7%) malignant tissues. ebv was not detected in either sera or tissue samples. adenovirus was evidenced in sera and tissues of one case of endometrial cancer.19921316844
unusual hla-b alleles in two tribes of brazilian indians.the kaingang and guarani are culturally and linguistically distinct tribes of southern brazil. like all amerindian groups they show limited hla polymorphism, which probably reflects the small founder populations that colonized america by overland migration from asia 11,000-40,000 years ago. we find the nucleotide sequences of hla-b alleles from the kaingang and guarani to be distinct from those characterized in caucasian, oriental and other populations. by comparison, the hla-a and c alleles are ...19921317015
[which serology tests for infection should be performed in a multi-organ donor?]. 19921317018
the role of occupational exposure and immunodeficiency in b-cell malignancies. working group on the epidemiology of hematolymphopoietic malignancies in italy.excesses of non-hodgkin's lymphoma have been observed among farmers exposed to phenoxyacetic acid herbicides and, less persuasively, among workers exposed to insecticides. exposure to organic solvents (particularly chlorinated hydrocarbons) has also been associated with an increased risk of nhl. tcdd (which is a contaminant of phenoxy herbicides), ddt, and chlorinated solvents have all been reported to induce impairment or suppression of cell-mediated immunity. we hypothesize that nhl is caused ...19921317221
herbal medicine use, epstein-barr virus, and risk of nasopharyngeal carcinoma.herbal medicine use is thought to be linked to nasopharyngeal carcinoma (npc) either through its ability to reactivate the epstein-barr virus (ebv) or through a direct promoting effect on ebv-transformed cells. to investigate this, 104 histologically confirmed npc cases and 205 matched controls were studied in the philippines. blood was collected to assess antibody titers against ebv, and an interview was administered which elicited information concerning herbal medicine use and other risk facto ...19921317256
[establishment and characteristics of a lymphoblastoid cell line (ckm) resistant to 6-tg and ouabain].three lymphoblastoid cell lines (ckm-c, ckm-1 and ckm-8) have been established by ebv-transformation from cord blood. they have been maintained for 1 year after 120 passages with rapid growth. the population doubling times were 24.5h (ckm-1), 30 h (ckm-8) and 36 h (ckm-c). the b lymphocyte features were identified under an electron microscope. ebv antigens including ebna, vca-ig a, ma and ea were negative in all the cell lines. the median chromosome number was 48-50. interferon (ifn) was induced ...19921317271
oral hairy leukoplakia. 19921316883
epstein-barr virus latent and replicative gene expression in oral hairy leukoplakia.oral hairy leukoplakia is an epithelial lesion of the tongue associated with productive infection by epstein-barr virus (ebv). however, no data concerning the pattern of ebv latent gene expression have been reported, and it remains unresolved whether true latent infection occurs in basal cell layers of oral hairy leukoplakia. we have studied six cases of oral hairy leukoplakia using monoclonal antibody immunohistology for ebv latent--eb nuclear antigen (ebna) 1, ebna 2 and latent membrane protei ...19921316871
ebv-genome positive monoclonal b cell cerebral lymphoma in a renal allograft recipient following okt3 therapy. 19921317529
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