[effect of sex on immunological responsiveness]. 1979230910
[epstein-barr virus and human chromosomes: close association of the resident viral genome and the expression of the virus-determined nuclear antigen (ebna) with the presence of chromosome 14 in human/mouse hybrid cells (author's transl)].fourteen hybrid clones derived from the fused cultures of human lymphoblastoid fv5 cells and 5-bromodeoxyuridine-resistant mouse fibroblastic mcb2 cells grown in hat selective medium were examined for the presence of epstein-barr virus (ebv) dna, the expression of the virus-determined nuclear antigen (ebna), and the presence of human chromosomes, in the course of serial passage in vitro. among the hybrid clones tested, three were positive for ebv dna and ebna, whereas the remaining 11 clones wer ...1978230998
[epstein-barr virus and human cells: transforming and cell-killing infections (author's transl)]. 1978230999
epstein-barr virus specific iga serum antibodies in nasopharyngeal and other respiratory order to better understand the relationship between iga and igg antibodies to epstein-barr virus (ebv) in nasopharyngeal carcinoma (npc), we analyzed 230 npc sera but also a series of sera from patients with other carcinomas selected for their high ebv/igg antibody titres. we were surprised to find that 21 out of 46 sera from bronchopulmonary carcinomas (bpc) and 6 out of 7 carcinomas of nasal fossae were iga anti-vca positive, and 9 bpc sera and 5 nasal fossae sera were also positive for iga ...1979231014
antibody-dependent cell-mediated cytotoxicity against epstein-barr virus membrane antigen in african burkitt's lymphoma.serial samples of sera from patients with african burkitt's lymphoma were tested for antibody against epstein-barr virus (ebv)-specific membrane antigen (ma) by the antibody-dependent cell-mediated cytotoxicity assay (adcc). titers of patients in the long-term survivor group were generally higher than those found in the sera of patients in the short-term survivor group. although adcc titers to ebv-ma were not useful in predicting which patients would relapse there was a definite relationship bet ...1979231015
blocking of acid-fixed nuclear binding of epstein-barr virus nuclear antigen (ebna) by different dna species.treatment of epstein-barr virus-determined nuclear antigen (ebna) with dna resulted in blocking of its ability to convert acid-fixed ebna-negative cell nuclei to an ebna-positive form. epstein-barr virus (ebv) dna, herpes simplex virus type 2 (hsv-2) dna and dna isolated from three lymphoblastoid cell lines differed in their potency to block this reaction. ebv dna was found to be about three times more effective than cellular dnas in abolishing the ability of dna-cellulose-purified ebna to conve ...1979231093
psychosocial risk factors in the developmental of infectious a 4-year prospective seroepidemiological study of infectious mononucleosis (im) of one class of some 1400 cadets at the west point military academy, susceptibles and immunes were identified by the absence or presence of antibody to epstein-barr virus (ebv), the causative agent, and new infections by the appearance of antibody (seroconversion). on entry, about 1/3 lacked ebv antibody, of whom some 20% became infected (seroconverted); about 1/4 of seroconverters developed definite, clinical and ...1979231279
[the molecular biology of herpes viruses: (part ii) dna structure analysis and latent viral genomes of ebv, mdv, h. saimiri, cmv etc. (author's transl)]. 1979231281
infectious mononucleosis and agranulocytosis.infectious mononucleosis is often complicated by haematological abnormalities but agranulocytosis is so rare that a causal relationship has been questioned. we here describe 2 sisters, 3 and 5 years old, who both developed agranulocytosis or profound granulocytopenia 4-5 weeks after the acute onset of mononucleosis. no contributory cause could be found and we conclude that a constitutional disposition may be responsible for this complication.1979231301
prevalence of antibodies to soluble antigen of epstein-barr virus-producing cells (p3hr-1) in the sera of hamadryas baboons of the high lymphoma incidence stock.soluble antigen of p3hr-1 cells (sa-p3hr-1) was identified in indirect double immunodiffusion enhanced with tannic acid using serum of a nasopharyngeal carcinoma patient containing high-titer antibodies to epstein-barr virus (ebv) antigens. sa-p3hr-1 was nonidentical to soluble antigen of raji cells. human and baboon sera containing antibodies to all the known antigen of ebv and hvp respectively were anti-sa-3hr-1-positive. human and baboon sera containing antibodies to all the known antigens of ...1979231520
surface markers and functional characteristics of human blood lymphocytes residing in the operational "null" subset.after removal of nylon wool-adherent b and e rosetting t cells from the human blood lymphocyte population, a small fraction remains, often denoted as "null" subset. this fraction contains lymphocytes which are highly efficient in antibody-dependent and non-selective lymphocyte cytotoxicity. these cells possess markers. about half of them carry fc receptors, and the majority has low avidity e and c3 receptors. removal of low avidity e receptor-carrying cells did not abolish cytotoxicity to k-562 ...1978231548
cancer, viruses and environmental factors.the genesis of burkitt's lymphoma is visualized as proceeding in three steps: i. primary ebv infection affects the young child, probably at a relatively high multiplicity. it immortalizes a certain number of b-lymphocytes in vivo. ii. this is followed by the impact of an environmental promoting agent, perhaps chronic holoendemic malaria, providing a chronic stimulus to the proliferation of the ebv-carrying preneoplastic cells. iii. chromosomally abnormal variants appear in the stimulated tissue ...1978231549
[epstein-barr virus (ebv) infection of epithelial cells from the nasopharynx--the use of autoradiography (author's transl)]. 1979231647
[significance of virus-specific antibodies to ebv-antigens in patients with nasopharyngeal carcinoma (author's transl)].sera collected from patients with nasopharyngeal carcinoma, patients with other head and neck neoplasms, patients with infectious mononucleosis (im), and healthy donors were titrated for antibodies (igm, igg, iga) against epstein-barr-virus (ebv) specific antigens (vca, ea, ebna) by indirect and by antikomplementary immunfluorescence. npc-patients develop significantly high anti-ebv titers of igg and iga. in contrast patients with neoplasms (mainly carcinomas) arising in sites of the head and ne ...1979231716
reactivity of paul-bunnell type heterophile antibody in sera from infectious mononucleosis patients with the surface of lymphoid cells carrying epstein-barr virus genomes.the possible presence of paul-bunnell (pb) antigen on epstein-barr virus (ebv)-transformed lymphocytes were investigated. of 23 ebv genome-positive lymphoblastoid cell lines tested all but one absorbed pb type antibody from the serum of an infectious mononucleosis patient. the one ebv-negative b cell line tested did not absorb the heterophile antibody. pb antibody, purified by an immunoadsorbent procedure using beef cell antigen, reacted with the ebv producer p3hr-1 cell line in an indirect memb ...1979231730
hodgkin's disease and infectious mononucleosis.sequential sero-positive infectious mononucleosis is described in 3 sibs, 2 of whom subsequently developed hodgkin's disease. eb virus antibodies were present in the serum of both cases.1979231818
a serological survey of st lucia.a serological survey of a random sample of 541 of the population of st lucia was undertaken. the prevalence of antibodies to dengue, herpes virus, vz, rotavirus, rubella and syphilis is described and compared with other communities.1979232090
intracellular state of epstein-barr virus dna in producer cell lines.the physical state of the epstein-barr virus (ebv) dna in three cell lines which spontaneously produce virus has been characterized. circular ebv dna molecules have been found in p3hr-i, b95-8 and m8i cells. the size of the intracellular m8i circular ebv dna molecules is comparable with the linear virus genome isolated from virus particles but the circular p3hr-i and b95-8 dna molecules are shorter than the virion dna . in addition to the circular form, some ebv dna with physical properties indi ...1979232128
identification of transcribed regions of epstein-barr virus dna in burkitt lymphoma-derived cells.rna was extracted from the burkitt lymphoma-derived cell line raji and from burkitt lymphoma tumor biopsies, isotope labeled in vitro by iodination with 125i, and hybridized to electrophoretically separated restriction endonuclease fragments of epstein-barr virus dna on nitrocellulose membranes. the results indicated that only certain parts of the epstein-barr virus genome are represented as polyribosomal rna in raji cells, with a pronounced dominance of rna sequences complementary to a 2.0 x 10 ...1979232190
[determination of igm and igg antibodies against the epstein-barr virus capsid antigen using the indirect immunofluorescence method (author's transl)]. 1979232207
sero-epidemiologic study of the epstein--barr virus infectivity in a healthy cuban population.the indirect immunofluorescence test was used to study the level of humoral antibodies against viral capsid antigen of the epstein--barr virus (vca-ebv), and early antigen (ea) in the sera of 322 healthy donors of the havana city province. the age of donors ranged from several months up to 95 years. the results obtained indicate that the healthy population of cuba is infected by ebv as well as in other countries, even though the spread and the course of infection have its specific characteristic ...1979232252
epstein barr virus (ebv) and complement (c3) interactions with human lymphoid cells. 1979232455
enzyme immunoassay for detection of antibody to epstein-barr virus-specific early antigen.antibody to epstein-barr virus-induced early antigen could be measured in sera using enzyme-linked antibody. the sensitivity of this assay was comparable to that of the indirect immunofluorescent technique. measurement of early antigen was accomplished on a producer cell line which was specifically treated to block late gene expression. it is now possible to measure epstein-barr virus-induced early antigen and viral capsid antigen antibodies by using the same cell line.1979232507
infection and persistence of varicella-zoster virus in lymphoblastoid raji cell line.infection of raji cells by varicella-zoster virus (vzv) resulted in permissive infection with establishment of a persistently infected lymphoblastoid cell line. vzv antigens of the membrane and nuclear type, as detected by the indirect immunofluorescence membrane antigen (ifama) and anticomplement immunofluorescence (acif) tests, were observed. minute amounts of infectious virus were detected by co-cultivation of vzv-infected raji cells (raji-vzv), with permissive human embryo fibroblasts (hef). ...1979232537
[angioimmunoblastic lymphadenopathy: ultrastructural study of lymph nodes. possible relationship with epstein-barr virus (ebv) (author's transl)].angioimmunoblastic lymphadenopathy (ail) has been clinically and histopathologically described by several authors since 1972, but only a few papers have analyzed the morphological aspects of the lymph nodes as observed by using electron microscopy. in the past three years, we happened to see in the curie institute two typical cases of ail, both of whose clinical and immunological data are briefly reported in this paper. we describe some peculiar patterns observed on the lymph nodes during the ac ...1979232552
mitogen-induced differentiation of human cll lymphocytes to antibody-secreting cells.peripheral blood lymphocytes from nine patients with chronic lymphatic leukaemia (cll) have been stimulated by photohaemagglutinin, pokeweed mitogen and epstein-barr virus. our results provide proof that the leukaemic b cells in cll are capable of responding to mitogenic signals by producing and secreting antibodies, transforming into blast cells, and, probably, increasing their rate of dna synthesis.1979232570
differentiation capacity and other properties of the leukemic cells of chronic lymphocytic leukemia. 1979232686
dna glycosylases of human lymphoblastoid cells.lymphoblastoid cell lines were established after transformation by epstein-barr virus of peripheral lymphocytes from xeroderma pigmentosum (xp) patients and normal donors. these lines expressed b-lymphocyte characteristics. typical characteristics related to xp of these cell lines were not altered by transformation. extracts of these cells catalyzed release of uracil (ura) and 3-methyladenine (3meade) from ura-containing dna (ura-dna) and methylated dna (me-dna), respectively. these two activiti ...1979232757
[network-theories and humoral immune response in infectious diseases (author's transl)]. 1979232825
cell-mediated immune responses in man to epstein-barr (eb) virus infection. 1979232867
the epidemiology of burkitt's lymphoma: evidence for a causal association with epstein-barr virus. 1979232877
the possible role of epstein-barr virus in rheumatoid arthritis.the sera of patients with rheumatoid arthritis form a precipitate with an antigen present in the sonicate of a human b (bone marrow-derived) lymphoid cell line (wil2). the antibody is distinct from rheumatoid factor. the antigen can be demonstrated by an immunofluorescence technique to be distributed within the nucleus of the wil2 cell and shows a discrete speckled pattern of nuclear staining. the nuclear antigen is not detectable in organ extracts from many animal species or in human t (thymus- ...1979233172
[application of immunoenzymic method and immunoautoradiographic method for the mass survey of nasopharyngeal carcinoma (author's transl)]. 1979233230
[detection of eb virus-specific iga antibody from patients with nasopharyngeal carcinoma by immunoenzymic method (author's transl)]. 1979233233
translocation 8;14 in an ataxia telangiectasia-derived cell line. 1979233260
phosphonoacetic acid: inhibition of transformation of human cord blood lymphocytes by epstein-barr virus.phosphonoacetic acid disodium salt (paa) inhibited the transformation of human cord blood lymphocytes by epstein-barr virus (ebv) at concentrations of 50-100 microgram/ml. at these concentrations, paa had no effect on the multiplication of ebv transformed human lymphoblastoid cells or on the survival of human cord blood lymphocytes. the transformation of human cord blood lymphocytes by the b95-8 strain of ebv was measured by 3h-thymidine uptake, 5 days or more after infection. the degree of inhi ...1979233303
[detection of iga antibody to eb virus vca from patients with nasopharyngeal carcinoma by immunofluorescence test (author's transl)]. 1979233359
[study of giant group a marker chromosome in several burkitt lymphoma and lymphoblastoid cell lines with epstein-barr virus from different origins (author's transl)]. 1979233360
[serological mass survey of nasopharyngeal carcinoma (author's transl)]. 1979233641
sister chromatid exchange in patients with viral disease.sister chromatid exchange (sce) frequencies were studied in differentially stained chromosomes from lymphocytes of 17 patients with viral disease. the mean sce score for the patients was 8.7 +/- 2.9 standard deviations. sce scores were significantly elevated in the patients compared with the controls (p less than 0.01); however, variability in sce means was observed in the patients. sce elevations were also present in long term cultured epstein barr virus positive human b lymphocytes.1978233771
antibodies to epstein-barr viral capsid and early antigens associated with burkitt's lymphoma and lymphoblastic lymphosarcoma in israel.before and/or after chemotherapy was administered to patients with burkitt's lymphoma (bl) or lymphoblastic lymphosarcoma (lls), their sera and those of matched controls were tested for antibodies to epstein-barr virus (ebv) capsid antigen (vca) and early antigen by the indirect immunofluorescence method. ten of the 16 bl patients were arab children and 8 of the 11 lls patients were jews of asian-african origin. although half the bl patients did not have elevated antibody titers when their disea ...1976233958
stability and dissociation of p3h4-1 burkitt's lymphoma cell soluble complement-fixing antigen identified with human serum.the soluble complement-fixing antigen of the p3hr-1 burkitt's lymphoma cell line, identified with human serum containing antibody against epstein-barr virus viral capsid antigen, loses activity under a variety of conditions. the major characteristic reported here is retention of activity on exposure to specific physical or chemical conditions followed by loss of activity after subsequent neutral dialysis. antigen of untreated cell lysates, which retains activity after dialysis against large volu ...1975236823
epstein-barr virus-induced cap formation in human lymphoblastoid cells.redistribution and consequent cap formation of epstein-barr virus adsorbed to human lymphoblastoid cells were studied by indirect membrane immunofluorescence carried out at 0 degrees c. when ebv was adsorbed on cells at 0 degrees c, the cell surface fluorescence had a mostly r-ng-like pattern. however, the ring cells could be transformed into cap cells when warmed at 37 degrees c. this cap formation could be induced by ebv alone without participation of antibodies involved in the immunofluoresce ...1975237838
purification of soluble complement-fixing antigens from two burkitt's lymphoma cell lines. conditions affecting the stability and recovery of antigens and antibodies.soluble epstein--barr virus (ebv) associated antigens of p3hr-1 and raji cell lines, which fix complement in the presence of human sera containing antibody against ebv--vca antigens, were partially purified by fractionating centrifuged cell lysates by the method of immunoadsorbents or by precipitation with ammonium sulphate or glycine--hcl. purification conditions were limited by the instability of both antigen and antibody activity. the antibody was inactivated under all the conditions commonly ...1975241721
sister chromatid exchanges and chromosomes in chronic myelogenous leukemia and cancer families.spontaneous sister chromatid exchanges and banded karyotypes were studied in blood lymphocytes from 96 individuals: seven patients with chronic myelogenous leukemia, 15 normal controls, and five "cancer families" comprising 12 cancer patients, 40 tumor-free blood relatives and 22 spouses. the families had: malignant melanoma; epstein-barr virus-associated malignancies and a birth defect syndrome; non-hodgkin lymphoma and diverse carcinomas; hodgkin's lymphoma and adenocarcinomas; and acute myelo ...1979282271
cytogenetic study of a european burkitt's lymphoma cell line.the chromosomes of an epstein-barr virus-negative european burkitt's lymphoma cell line were studied. all the cells carried the t(8;14) translocation. one clone had 51 chromosomes and was (+1,+7,+16,+15,+21), whereas another clone also had 51 chromosomes but was (+1q+,+7,+16,+15,+21). a third clone had 46 chromosomes (4q+/-).1979286095
nasopharyngeal and adjacent neoplasms: a clinico-pathologic and immunologic study.thirteen cases of nasopharyngeal carcinoma (npc) and 16 cases of non-npc tumors in the nasopharynx or in adjacent locations were investigated clinically, immunologically, and pathologically. all tumors were classified according to the tnm classification, and the stage and course of the disease was correlated with the histological tumor type, the t- and b-cell distribution in tumor tissue and in the peripheral blood, as well as with antibody titers against epstein-barr virus (ebv). the results sh ...1978305242
establishment of a t-cell line from human lymphosarcoma.a leukemic t-cell line, designated as tall-1, has been established in continuous culture from the bone marrow of a patient with a leukemic phase of t-cell lymphosarcoma. tall-1 cells have t-cell properties and lack epstein-barr virus or its genome. the cells of tall-1 line are considered to have originated from the donor's leukemic cells on the basis of their cytogenetic, morphological, and surface features.1978305872
rabbit antiserum against daudi-cell membrane fractions detects cell surface antigens present on subpopulations of human antiserum was produced by immunization of rabbits with membrane preparations of a human lymphoblastoid b cell line, daudi. after absorption with a human endometrial carcinoma cell line, this antiserum appeared to be specific for antigen(s) present on adult and fetal thymocytes as well as on tonsillar lymphocytes but absent, or present in very small amounts, on normal or phytohemagglutinin- (pha) stimulated peripheral blood lymphocytes (pbl). when t and b cell-enriched fractions from tonsillar ...1978307019
human t lymphocyte cell line producing colony-stimulating activity.we derived a permanent human t lymphocyte cell line that elaborates a potent colony-stimulating activity (csa). the line was established with spleen cells from a patient with a t lymphocyte variant of hairy-cell leukemia. these cells form rosettes with sheep erythrocytes, show a proliferative response to phytohemagglutinin, and are lysed by antithymocyte globulin. they do not synthesize immunoglobulin, nor do they contain epstein-barr virus. csa is regularly detected in the supernatant medium af ...1978308824
a histopathological study of lymphoid tissue reaction to metastatic nasopharyngeal carcinoma in nude attempts to heterotransplant human npc into nude mice, using seven cultured cell lines, we have succeeded in growing a carcinoma simplex, composed of epstein-barr virus-determined nuclear antigen-positive and epstein-barr virus genome-positive cancer cells, at the injected site with two of the cell lines. these originated from a spindle-cell carcinoma (chinese npc-204) and from a combined-cell carcinoma (chinese npc-501), respectively. during the first few passages, wandering macrophages were ...1978310417
cultured "hairy cells" infected with epstein-barr virus: evidence for b-lymphocyte origin. 1979311181
[t- and b-lymphocyte determination in infectious mononucleosis].in 12 children with infectious mononucleosis the peripheral blood t- and b-lymphocytes were studied and compared to a control group of 26 children, aged 2 to 13 years, without any infection. spontaneous sheep red blood cell rosettes were used as a t-cell marker, demonstration of surface immunoglobulins by immunofluorescence technique was used as a b-cell marker. patients with infectious mononucleosis showed a marked increase of t-lymphocytes (percentage and absolute numbers) in comparison with t ...1977328360
epstein-barr virus (ebv) receptors, complement receptors, and ebv infectibility of different lymphocyte fractions of human peripheral blood. i. complement receptor distribution and complement binding by separated lymphocyte subpopulations. 1978340053
[epidemiology of burkitt's lymphoma in tropical areas - its relationship with malaria (author's transl)].epidemiology of tropical burkitt's lymphoma shows the concomitant existence of the three following characteristics in endemic areas: - fairly dense rural population with a low standard of living; - early occurrence of primary infection by the epstein-barr virus (whose genome is always present in all tumour cells); - stable malaria. the social and economic level of these areas accounts for the specific features of the viral infection. climatic factors determine permanent of prolonged transmission ...1978345230
immunohistochemistry of local immunoglobulin production in nasopharyngeal carcinoma.immunohistochemical investigations by the immunoperoxidase method have been carried out on sections of biopsy specimens obtained from the primary tumour sites of patients with nasopharyngeal carcinoma (npc). it was found that, in many of the sections thus examined, there was an accumulation of plasma cells, particularly of the iga type, in the connective tissues surrounding nests of npc cells. similar accumulation of plasma cells in the subepithelial connective tissues was likewise observed in a ...1978348224
[a specific igm test for the diagnosis of epstein-barr virus infections (author's transl)].igm antibodies specific for epstein-barr virus (ebv) were measured in 302 patients with high igg antibody titers to study whether ebv was the cause of disease in children having one or more symptoms of classical infectious mononucleosis. igm antibodies specific for ebv were found in all patients with the defined clinical picture of infectious mononucleosis. in addition the majority of cases with clinical suspicion of the disease had also specific igm titers. besides infectious mononucleosis ebv ...1979390371
biologic and antigenic characteristics of epstein-barr virus-related herpesviruses of chimpanzees and baboons.leukocyte-transforming agents were isolated in baboon leukocytes inoculated with oral excretions from immunosuppressed chimpanzees. the transformed lymphoblasts had b cell surface markers and harbored herpes-type virus particles; 5-10% of the cells contained cytoplasmic antigens reactive with epstein-barr virus (ebv)-antibody-positive chimpanzee, human and baboon sera. these sera also neutralized the transforming activity of the chimpanzee virus. long-term lymphoid cell lines were established fr ...1977409690
acute non-a, non-b hepatitis. prolonged presence of the infectious agent in blood.non-a, non-b hepatitis, previously transmitted to chimpanzees by inoculation of human serum, was serially transmitted through a second and third passage to additional chimpanzees using serum drawn during acute non-a, non-b hepatitis. sera obtained at weeks 4 and 5 after inoculation from two different chimpanzees, and from one chimpanzee at week 13 after inoculation, were shown to cause elevation of serum aminotransferase levels and abnormal liver biopsies in recipient chimpanzees, with no serolo ...1979421994
cell-mediated lymphocytotoxicity directed against hla-d gene products.cytotoxic effector cells were generated in human mixed lymphocyte cultures (mlcs) between cell donors differing at the hla-d/dr region but not at hla-a,b(c). these cells killed epstein-barr virus-infected lymphoblasts (ebv blasts) from donors sharing the hla-d/dr determinants with the normal mlc-stimulating cells, but not phytohaemagglutinin (pha) lymphoblasts from the same donors. because ebv blasts are derived from b cells and express hla-d/dr antigens, the most likely interpretation of these ...1979432943
histological and electron microscopic studies of endemic ethmoidal carcinomas in five cases of endemic ethmoidal carcinoma in cattle from the dominican republic three tumor types could be classified: undifferentiated carcinoma (3), adenocarcinoma (1), and squamous cell carcinoma (1). electron microscopy showed that the tumor cells in undifferentiated carcinomas closely resembled the cells of the normal olfactory mucosa. this was especially true for the dark cells of bowman's gland. ultrastructurally, the lymphoid cells of the undifferentiated bovine carcinoma resembled th ...1979442448
non-a, non-b hepatitis among participants in a plasmapheresis stimulation program.non-a, non-b hepatitis was transmitted to seven of nine participants in a red blood cell-stimulation program following transfusion of blood from one asymptomatic donor. five of the seven patients had clinical as well as biochemical evidence of infection, and three of these five were icteric. incubation periods for the clinical cases ranged from 28 to 50 days, and duration of illness was from two to eight weeks. none of the seven patients showed serologic evidence of acute infection or reinfectio ...1979448190
association of human chromosome 14 with a ts defect in g1 of chinese hamster k12 cells.somatic cell hybrids between human lymphoblastoid cells (raji) and temperature-sensitive chinese hamster cells (k12) were selected from monolayer cultures in mem at 40 degrees c. a total of 21 hybrid clones were isolated and karyotyped. all clones contained a near complete set of chinese hamster chromosomes and 1 to 5 human chromosomes. human chromosome 14 present in the hybrid cells of all clones; and was the only human chromosome retained in 10 clones. the presence of human chromosome 14 in hy ...1979455493
infectious mononucleosis in the elderly.infectious mononucleosis (im) was diagnosed in four patients over the age of 50 years. their age, absence of splenomegaly, lack of significant lymphadenopathy in three and an atypical presentation in one all contributed to a delay in the diagnosis. in two patients, in whom complications occurred, the paul-bunnell test was repeatedly negative. confirmation of the diagnosis was made by the measurement of the epstein-barr virus igm using differential sucrose gradient centrifugation. because the pre ...1979463681
2/8 translocation in a japanese burkitt's lymphoma.a new translocation between chromosomes 2 and 8, t(2p-; 8q+), was found in fresh lymphoma cells from a japanese patient with epstein-barr virus-carrying burkitt's lymphoma, and in a lymphoma cell line derived from this patient. there was no 14q+ translocation, as has been previously described in african and north american burkitt's lymphomas.1979467575
remission of giant lymph node hyperplasia with anemia after radiotherapy.we have described a 51-year-old patient with unresectable mesenteric giant lymph node hyperplasia of the plasma cell type, severe systemic manifestations, and profound anemia. supression of erythropoiesis may have been related to the presence of a circulating erythropoietic inhibitor produced by the lymphoid tumor. markedly elevated titers to epstein-barr virus capsid antigen suggest that this virus may be important in the etiology of the abnormal lymphoid proliferation. the marked clinical resp ...1979476562
cell culture studies in progeria. i. establishment and partial characterization of a lymphoblastoid cell line.a lymphoblastoid cell line (eks-l44) with definitive hla specificities (hla-aw24, w30, b14, w35, cw4-, drw4, 5) was established from peripheral blood cells of a patient with progeria using epstein-barr virus. cytogenetic study indicated that eks-l44 was diploid after four months in vitro. the growth pattern of this cell line was not significantly different from a similarly established normal control nor from other lines initiated from patients with various disorders.1979513854
[carcinoma of the nasopharynx in the young subjects. clinical, pathological and ultrastructural study of 50 cases in eastern algeria (author's transl)].africa, and in particular north africa, is a site of high prevalence of carcinoma of the nasopharynx in the young subject. there is a marked male predominance and a clear peak of incidence between 15 and 25 years. the presenting feature is usually rapidly growing cervical lymphadenopathy. the diagnosis is based upon histological examination of these nodes. it is a squamous carcinoma, usually highly undifferentiated and difficult to identify. however, for the experienced observer, the special app ...1977600721
studies in porphyria. vii. induction of uroporphyrinogen-i synthase and expression of the gene defect of acute intermittent porphyria in mitogen-stimulated human lymphocytes.a 50% reduction in the activity of uroporphyrinogen-i (uro) synthase in liver, erythrocytes, and cultured skin fibroblasts characterizes all patients with clinically active acute intermittent porphyria (aip). the same enzyme defect has also been demonstrated in the erythrocytes and skin fibroblasts of completely latent gene carriers of this disorder and presumably exists in the liver as well. in this study, we examined whether or not the formation of uro-synthase is impaired in aip cells using l ...1978621286
fatal aplastic anaemia complicating infectious mononucleosis.a 7-year-old boy developed fatal aplastic anaemia, 2 weeks after the first signs of infectious mononucleosis. although some drugs were administered, infection with the epstein-barr virus, leading to infectious mononucleosis, is assumed to be the cause of this aplastic anaemia. some mechanisms possibly leading to the aplasia are discussed.1978625631
infectious mononucleosis associated with rhabdomyolysis and renal failure.acute rhabdomyolysis and renal failure occurred in a 16-year-old boy with infectious mononucleosis; recovery was complete. renal failure is a rare complication of infectious mononucleosis. rhabdomyolysis has been reported only once in association with the epstein-barr virus, but has frequently been associated with influenza virus infections. prospective studies measuring creatine phosphokinase enzymes should establish the frequency of rhabdomyolysis in viral infections.1978628861
anti-viral activity induced by culturing lymphocytes with tumor-derived or virus-transformed cells. identification of the anti-viral activity as interferon and characterization of the human effector lymphocyte subpopulation.a viral inhibitor(s) is released in the supernate of mixed cultures containing human or mouse lymphocytes and cells from certain lines. the inhibitor is active against a variety of unrelated viruses and is a protein that is not toxic for cells. it does not inactivate viruses directly, but inhibits viral replication through an intracellular mechanism that involves synthesis by the cells of both rna and protein. these characteristics identify the inhibitor as an interferon. the anti-viral activity ...1978650155
[bcg vaccination, cancer and infectious mononucleosis. a retrospective study of hospitalized patients].a retrospective study (1966--1976) of patients hospitalized for cancer was carried out to determine a possible relationship with bcg vaccination, with a control group matched for age and sex and a similar study concerning infectious mononucleosis. among 105 patients with cancer, 54 (51.42%) had been previously vaccinated with bcg, 56 (53.33%) in the control group, and 22 (33.84%) among the 65 patients with infectious mononucleosis (p less than 0.05, x2). the mean age on admission for cancer or i ...1978711498
fever of undetermined origin: role of cytomegalovirus and epstein-barr virus.roles of various infections, neoplasms and granulomatous diseases in fever of unknown origin were studied in 22 patients. bacterial infections were responsible in seven (32 percent), herpes viruses in five (23 percent), neoplasms in four (18 percent) and granulomata in two (9 percent) cases. patients with herpes infections had initially no clinical or routine laboratory clues to viral involvement, and were given an intensive workup for 5 to 15 days. the diagnosis was made on the basis of cytomeg ...1978716387
transcription and circular dichroism of chromatin in lymphoblastoid cell lines during proliferation and quiescence.rna polymerase activity of nuclei and positive circular dichroism ellipticity in the 250--300 nm region of chromatin were studied in three lymphoblastoid cell lines: hri, an epstein-barr virus (ebv) positive virus-producing line; raji, and ebv positive virus-producing line; raji, an ebv positive non-producer line; and su-amb-1, and ebv negative line. raji consistently exhibited the highest and su-amb-1 the lowest polymerase activity and ellipticity. all lines showed higher rna polymerase activit ...1978718933
spontaneous resolution of severe aplastic anemia associated with viral hepatitis a in a 6-year-old child.recovery from acquired aplastic anemia associated with hepatitis is rare. this case of a 6-year-old boy with severe aplastic anemia is the first reported association of this disease with a hepatitis a infection. antibody to hepatitis a (anti-ha) was not detected on admission, but was detected three weeks later. infection with hepatitis b virus, cytomegalovirus (cmv), and epstein-barr virus (ebv) were excluded. the peak titer of anti-ha was higher than would be expected for passive transfer of an ...1978752262
[relation of antibody levels to epstein-barr virus to histological variant and clinical course of lymphogranulomatosis].the indirect immunofluorescent test in fixed p3hr-1 cells showed the sera from patients with lymphogranulomatosis to contain high titers of humoral antibody to the capsid antigen of epstein-barr virus. higher titers of antibody to ebv were associated with unfavourably running histological variants of lymphogranulomatosis. the lymphoid depletion variant was accompanied by high antibody titers to the virus and had a worse prognosis than the nodular-sclerotic variant with lower antibody titers but ...1976769339
[serological studies of antibodies to epstein-barr virus in infectious mononucleosis].this is the first study in this country of sera from patients with infectious mononucleosis and other infectious diseases (control group) for determination of complement-fixing antibody to the soluble (s) antigen and of fluorescent antibody to the virus capsid antigen (vca) of epstein-barr virus. it was established that the cft and the immunofluorescence test could be used for diagnosis of infectious mononucleosis only on a limited scale because of the lack of complement-fixing antibody in patie ...1976779272
[humoral antibodies to epstein-barr virus in infectious mononucleosis].a high level of humoral antibody to the capsid antigen of epstein-barr virus was found in sera from patients with infectious mononucleosis. for detection of antibody, the method of indirect immunofluorescence in fixed p3hehr cells of burkitt's lymphoma was used. sera from normal subjects were also shown to contain antibody to the capsid antigen of epstein-barr virus, but the titers were 5--10 times lower than in the sera from infectious mononucleosis patients. the occurrance of high titers of an ...1976779273
renal failure in infectious 18-year-old man developed generalized lymphadenopathy and interstitial renal disease which progressed to irreversible renal failure. serologic studies evidenced the recent onset of infectious mononucleosis, and prior exposure to epstein-barr virus was demonstrated. lymph node, liver, and renal biopsies showed mononuclear cell infiltration consistent with infectious mononucleosis. although renal involvement in infectious mononucleosis rarely is severe, an association between infectious mononuc ...1976788163
properties of the k562 cell line, derived from a patient with chronic myeloid leukemia.the k562 cell line derived from a cml patient in blast crisis was examined for properties of b and t lymphocytes and cell lines. k562 lacks the b markers of immunoglobulins, epstein-barr virus (ebv) genome and associated nuclear antigen, and receptors for ebv. a low proportion of cells from rosettes with sheep erythrocytes, the frequency of which is considerably increased after neuraminidase treatment. unlike b lines but like t lines, k562 cells are lysed rapidly by c'/fc receptor-positive human ...1976789258
[herpes virus in cell cultures of the hematopoietic organs of hamadrymas baboons with hemoblastosis].five transplantable suspension lymphblastoid cell cultures from haematopoietic organs of baboons with malignant lymphoma were obtained, designated as kmpg-1, spg-1, 2, 3, 4. the cells of these suspension cultures contain hvp virus belonging to herpes-type according to morphological (electron-microscopic) investigation, but differing from infectious viruses of this group and closely related to epstein-barr virus as evidenced by its characteristics. in conformity with the preliminary data the isol ...1976818808
continuous lymphoblastoid suspension cultures from cells of haematopoietic organs of baboons with malignant lymphoma--biological characterization and biological properties of the herpes virus associated with culture cells.the continuous lymphoblastoid cell lines kmpg-1 and spg-2 producing baboon herpes virus (hvp) with morphological signs of herpes viruses have been established from haematopoietic organs of lymphomatous baboons. as distinct from h.simplex type i and ii viruses hvp is not pathogenic for laboratory rodents. according to its physico-chemical and immunological properties hvp is related to the epstein-barr virus.1976825381
[post-transfusion hepatitis 1973 and 1974 in the zürich region].more than 100 000 units of blood were tranfused during the years 1973-74 in the region of zurich. 30 cases of posttransfusion hepatitis (pth) reported in this period were investigated with regard to the origin of the tranfused blood, and 24 (71%) proved to be hepatitis b antigen (hbs-ag) positive. despite the use of a sensitive method for hbs-ag screening, transfusion of hbs-ag positive blood was found to be responsible for pth in 4 cases only. in 88% of the cases there was no proof that the pth ...1977834982
hematopathology and pathogenesis of the x-linked recessive lymphoproliferative syndrome.subtle immunodeficiency to infectious agents including measles virus and ten epstein-barr virus (ebv) has been described in the x-linked recessive lymphoproliferative syndrome. this syndrome has affected six male cousins and possibly another boy. three brothers died of an infectious mononucleosis syndrome, in a maternal cousin agammaglobulinemia developed three years after infectious mononucleosis, and two half-brothers of the duncan kindred died of lymphoma of the brain and intestinal tract, re ...1977835602
[neurological complications of infectious mononucleosis (author's transl)].the authors report three different and rare forms of neurological complication associated with infectious mononucleosis. the first two, which are faily unusual in their clinical signs, occurred during the course of two typical cases of mononucleosis, one in the form of progressively regressive myelitis and the other in that of amyotrophic paralysis of the shoulder. the third occurred in isolation, after an encephalitic attack combined with a state of epileptic mal, with no other accompanying inf ...1977842275
chromosome 14 translocation in african and north american burkitt's lymphoma;.two established north american burkitt lymphoma cell lines were studied by chromosomal banding techniques. the su-amb-1 line previously shown to be negative for the epstein-barr virus (ebv) was found to have, among other changes, a translocation from the long arm (q) of chromosome 8 onto 14q. the su-amb-2 line, which contains the ebv genome, also displayed the same 8/14 translocation. these results were compared with data from three ebv-positive tumor cell lines derived from patients with africa ...1977844916
a new class of infectious agents detectable by the production of chorioallantoic membrane lesions by human lymphoblastoid cell lines and their culture supernatants.intravenous injection of cells and their tissue culture supernatants (cs) from human lymphoblastoid cell lines (lcl) induced the formation of lesions on the chorioallantoic membrane (cam) of the chicken embryo. injection of cells and cs from non-lcl and normal human lymphocytes induced few or no lesions. irradiated chick embryos were more sensitive to lesion formation than were nonirradiated embryos. the log10 cam lesions induced in irradiated (500 rads) embryos were a linear function of the log ...1977845990
burkitt's lymphoma in australian case of burkitt's lymphoma is reported. the clinical features of large ovarian masses and subsequent bone marrow invasion, as well as the results of investigations related to the epstein-barr virus, were more consistent with the american than the african type of burkitt's lymphoma. after a good initial response to cyclophosphamide and vincristine, the tumour rapidly became resistant to these and other therapeutic measures and the patient died 10 months after diagnosis.1977859483
sequential evaluation of cutaneous delayed hypersensitivity responses to recall and to lymphoid cell line antigens in burkitt's lymphoma.delayed cutaneous hypersensitivity reactions to standard recall antigens (candidin, mumps and ppd), to crude membrane extracts of a cell line derived from burkitt's lymphoma (raji) and to cell line derived from normal lymphocytes (f265) were sequentially evaluated in 44 patients with burkitt's lymphoma. sixteen patients (36%) manifested delayed hypersensitivity responses to the standard antigens and seven (16%) to the raji membrane extract at presentation. following successful chemotherapy, ther ...1977903183
[serologic survey for the determination of antibodies against various virus infections, mycoplasma, beta hemolytic a streptococcus and toxoplasma gondii, performed on children of a state-of-mexico municipality].a seroepidemiologic inquest was carried out in 667 children from the county of huixquilucan, mexico, looking for serum antibodies against infections caused by the following microorganisms: epstein-barr virus; cytomegalovirus; syncytial respiratory; para-influenza 1, 2 and 3; measles; rubella; mumps; mycoplasma pneumoniae, beta hemolytic a streptococcus and toxoplasma gondii. the findings are reported. considerations are made on the incidence of these diseases in different age groups and a compar ...1977911440
preferential inhibition of herpes-group viruses by phosphonoacetic acid: effect on virus dna synthesis and virus-induced dna polymerase activity. 1976960726
survey of human lymphoblastoid cell lines and primary cultures of normal and leukaemic leukocytes for oncornavirus production.velocity sedimentation of uridine-labelled cultures was found to be more reliable than isopycnic sedimentation in detecting oncornavirus production in lymphoid cells. of 13 cell lines (including six derivea from burkitt's lymphomas and two from leukaemic leukocytes) only one, the leukaemia-derived, epstein-barr virus-producing line qimr-wil, showed any activity. the nature of the qimr-wil particles was further defined by isolation of uridine-labelled 70s rna and by the simultaneous assay for rev ...1976977188
disparity in the production of lymphoblastogenesis inhibition factor by cultured human b and t lymphoid cell lines.a comparative study of inhibitory effects of cell-free supernatants from cultured human b and t lymphoid cell lines on lymphocyte blastogenesis indicated that the inhibitory effect of supernatant from b lymphoid cells on lymphocyte blastogenesis was significantly higher than that of supernatant from t lymphoid cells or from non-lymphoid neoplastic cells. the inhibitory effect of supernatant was reversible and dose-related. the inhibitory effect gradually diminished with time when the supernatant ...19751081931
the american burkitt lymphoma registry: a progress report.the american burkitt lymphoma registry (ablr) investigates the cause of burkitt's lymphoma through comparative studies involving endemic and nonendemic regions. in 3 years 114 cases have been confirmed. diagnosis was based on evaluation of pathologic materialy submitted to ablr pathologists. similarities between american and african patients included abrupt clinical presentation with involvement of the gastrointestinal tract, jaw, gonads, and central nervous system. response to high-dose cycloph ...19751147436
lectin agglutinability of non-neoplastic and neoplastic human lymphoid cells in vitro.agglutination by two lectins, concanavalin a (con a) and ricinus communis agglutinin (rca), has been investigated in a human lymphoid cell system. the main conclusions of this study are: (1) no systematic correlation exists between the neoplastic state and sensitivity to con a or rca; (2) cells of neoplastic lines vary unsystematically in their surface properties as evaluated by con a agglutination, with the possible exception that presence of epstein-barr virus (ebv) is associated with a high d ...19751150344
cell-mediated immunity in human acute leukemia.the general cell-mediated immunological reactivity of patients with acute leukemia has been found to be intact, although it may be depressed by extensive disease or by chemotherapy. patients with acute leukemia also have cellular immune reactivity against tumor associated antigens, as measured by skin tests for delayed hypersensitivity, lymphocyte stimulation, and 51cr release cytotoxicity. skin reactions to autologous and allogeneic crude membrane extracts of blast cells correlated with disease ...19751164404
[epidemiology of cancer of the cavum].while rare in most parts of the world, cancer of the nasopharynx is shown to be very common in southern chinese whether living in south china or elsewhere, is of intermediate level in a variety of mongoloid populations such as malays, thais, javanese and vietnamese and the indigeneous peoples of borneo, yet uncommon in north china and very rare in japan. moderate frequencies are seen in parts of the maghreb, malta, the sudan and possibly east africa. occupational risk has not been demonstrated a ...19751191785
the emerging pattern of post-transfusion hepatitis.the exclusion of commercial and hepatitis b surface antigen (hbs ag)-positive donors, as measured by counterelectrophoresis, has markedly reduced the frequency of post-transfusion hepatitis (pth). a further, significant reduction in type-b pth can be achieved by prescreening donors for hbs ag by solid-phase radioimmunoassay (ria); when a voluntary donor population, pretreated by ria, is used, approximately 90 per cent of residual hepatitis is serologically unrelated to either the type-a or type- ...19751235474
sinus histiocytosis with massive lymphadenopathy: fifteen new cases.fifteen cases of chronic lymphadenitis with massive hemophagocytic sinus histiocytosis were analyzed. fourteen patients were whites, 13 were europeans, and 11 were males; 10 patients were under 10, 4 were over 20, and 2 over 60 years old. the oldest patient died; all other either healed without therapy or are in excellent condition. we studied the cytologic features of lymph node imprints. a 5-year-old girl was examined more thoroughly. high antibody titers to klebsiella antigens were found repe ...19761253107
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