PMID(sorted descending)
comparison of the artus epstein-barr virus (ebv) pcr kit and the abbott realtime ebv assay for measuring plasma ebv dna loads in allogeneic stem cell transplant recipients.the ability of the artus epstein-barr virus (ebv) pcr kit and the abbott realtime ebv pcr assay to detect and quantify plasma ebv dnaemia was compared. the agreement between these assays was 95.8%. the ebv dna loads measured by the two assays significantly correlated (p=< 0.0001).201728274502
hodgkin lymphoma in a case of chronic myeloid leukemia treated with tyrosine kinase inhibitors.chronic myeloid leukemia (cml) is characterized by increased and unregulated proliferation of granulocytic lineage in the bone marrow and presence of these immature myeloid cells in the peripheral blood with presence of philadelphia (ph) chromosome. tyrosine kinase inhibitors are the most important drugs in the cml therapy and provide long disease-free survival. due to the increased survival of cml patients with continual administration of these drugs, the chance of development of secondary mali ...201728272671
efficient and reliable establishment of lymphoblastoid cell lines by epstein-barr virus transformation from a limited amount of peripheral blood.lymphoblastoid cell lines (lcls) transformed by epstein-barr virus (ebv) serve as an unlimited resource of human genomic dna. the protocol that is widely used to establish lcls involves peripheral blood mononuclear cell isolation by density gradient centrifugation, however, that method requires as much as 5 ml of peripheral blood. in this study, in order to provide a more simple and efficient method for the generation of lcls, we developed a new protocol using hemolytic reaction to enrich white ...201728272413
understanding epstein-barr virus life cycle with proteomics: a temporal analysis of ubiquitination during virus reactivation.epstein-barr virus (ebv) is a human γ-herpesvirus associated with cancer, including burkitt lymphoma, nasopharyngeal, and gastric carcinoma. ebv reactivation in latently infected b cells is essential for persistent infection whereby b cell receptor (bcr) activation is a physiologically relevant stimulus. yet, a global view of bcr activation-regulated protein ubiquitination is lacking when ebv is actively replicating. we report here, for the first time, the long-term effects of igg cross-linking- ...201728271981
time correlation between mononucleosis and initial symptoms of determine the average age of ms onset vs the age at which epstein-barr infection has previously occurred and stratify this analysis by sex and the blood level of epstein-barr nuclear antigen 1 (ebna1) antibody.201728271078
the autophagic machinery in viral exocytosis.the discovery of the molecular machinery of autophagy, namely atg proteins, was awarded with the nobel prize in physiology and medicine to yoshinori ohsumi in 2016. while this machinery was originally identified by its ability to allow cells to survive starvation via lysosomal degradation to recycle cellular components, it has recently become apparent that it also is used by cells to secrete cytoplasmic constituents. furthermore, viruses have learned to use this atg supported exocytosis to exit ...201728270807
metastatic epstein-barr virus-positive lymphoepithelioma-like cholangiocarcinoma in a young man with ulcerative colitis. 201728270680
plasma epstein-barr virus dna for pediatric burkitt lymphoma diagnosis, prognosis and response assessment in malawi.point-of-care tools are needed in sub-saharan africa (ssa) to improve pediatric burkitt lymphoma (bl) diagnosis and treatment. we evaluated plasma epstein-barr virus (pebv) dna as a pediatric bl biomarker in malawi. prospectively enrolled children with bl were compared to classical hodgkin lymphoma (chl) and nonlymphoma diagnoses. pediatric bl patients received standardized chemotherapy and supportive care. pebv dna was measured at baseline, mid-treatment, and treatment completion. of 121 assess ...201728268254
whole-body (18)f-fdg pet/ct for m staging in the patient with newly diagnosed nasopharyngeal carcinoma: who needs?although whole-body fluorine-18 fluorodeoxyglucose ((18)f-fdg) positron emission tomography (pet)/computed tomography (ct) ((18)f-fdg pet/ct) is commonly used for m staging of newly diagnosed nasopharyngeal carcinoma (npc), some patients may not benefit from this procedure. the present study investigated which patients require this modality for m staging.201728267540
novel mutations in sh2d1a gene in x-linked lymphoproliferative syndrome, diagnosed after b-cell non-hodgkin lymphoma.x-linked lymphoproliferative disease type i (xlp i) is caused by mutations in the sh2d1a gene and characterized mainly by hypogammaglobulinemia and abnormal response to epstein-barr virus with a high predisposition to b-cell non-hodgkin lymphoma development.201728267077
epstein-barr virus and cytomegalovirus infections and their clinical relevance in egyptian leukemic pediatric patients.epstein-barr virus (ebv) and human cytomegalovirus (cmv) infections are environmental risk factors affecting the outcome of cancer due to an impairment in the cell-mediated immunity. therefore, this study aimed to detect the frequency of ebv and cmv dna and their association with clinical characteristics and outcome of pediatric leukemic patients.201728264674
psychosis in a 15-year-old female with herpes simplex encephalitis in a background of mannose-binding lecithin deficiency.historically, psychotic disorder has been associated with viral infection. herpes simplex infections and epstein-barr virus (ebv) among other viral infections have been implicated in psychotic disorder. of note in this case report is psychotic disorder that occurred following reactivation of herpes simplex infection in a background of mannose-binding lecithin (mbl) deficiency, childhood ebv infection, and severe psychosocial stress. herpes simplex encephalitis (hse) remains a significant cause o ...201728261514
pretreatment alkaline phosphatase and epstein-barr virus dna predict poor prognosis and response to salvage radiotherapy in patients with nasopharyngeal carcinoma and metachronous bone-only metastasis.background : the bones are the most common site of distant metastasis in nasopharyngeal carcinoma (npc). few prognostic markers are available to guide treatment and sub-classify patients with bone metastasis. we aimed to identify the prognostic value of pretreatment serum alkaline phosphatase (alp) and plasma epstein-barr virus dna (ebv dna) in patients with bone-only metastasis. methods : a total of 272 patients who developed bone-only metastases after therapy were retrospectively analyzed. pat ...201728261343
peritonsillar abscess: clinical aspects of microbiology, risk factors, and the association with parapharyngeal abscess.pta is a collection of pus located between the tonsillar capsule and the pharyngeal constrictor muscle. it is considered a complication of acute tonsillitis and is the most prevalent deep neck infection (approximately 2000 cases annually in denmark) and cause of acute admission to danish ent departments. teenagers and young adults are most commonly affected and males may predominate over females. however, no studies of age- and gender-stratified incidence rates have previously been published. fu ...201728260599
berberine inhibits the proliferation of human nasopharyngeal carcinoma cells via an epstein-barr virus nuclear antigen 1-dependent mechanism.nasopharyngeal carcinoma (npc) is a malignancy derived from the epithelial cells of the nasopharynx cavity, and is closely associated with epstein-barr virus (ebv) infection. in addition to npc, ebv causes various human malignancies, such as gastric cancer, hematological tumors and lymphoepithelioma-like carcinomas. epstein-barr nuclear antigen 1 (ebna1) encoded by ebv is indispensable for replication, partition, transcription and maintenance of viral genomes. berberine, a naturally occurring is ...201728259949
dissecting the regulation of ebv's bart mirnas in carcinomas.epstein-barr virus (ebv) encodes multiple mirnas known to contribute to its pathogenicity. previous studies have found that the levels of some ebv mirnas are 10-100 fold higher in biopsies and in tumor xenografts than in cells grown in culture. we have asked if these increased levels reflect transcriptional enhancement resulting from the tumor microenvironment, selection for increased levels of the ebv genome, or both. we measured the levels of bart mirnas and their dna templates in tumor xenogr ...201728259048
analysis of bzlf1 mrna detection in saliva as a marker for active replication of epstein-barr virus.monitoring replicative epstein-barr virus (ebv) infection still remains a challenge in modern laboratory routine. the immediate-early protein bzlf1 mediates the switch between latent and replicate forms of ebv infection. the aim of this study was to analyze the feasibility of bzlf1 mrna detection in saliva as a marker for active replication of the virus. various specimens (saliva, plasma, pbmc) from 17 patients with ebv-induced infectious mononucleosis (im) and 4 control patients were examined f ...201728257801
computed tomography findings in epstein-barr virus (ebv)-positive diffuse large b-cell lymphoma (dlbcl) of the elderly: comparison with ebv-negative dlbcl.this study aimed to compare ct findings in patients with epstein-barr virus positive (ebv+) diffuse large b-cell lymphoma (dlbcl) of the elderly with ct findings in patients with epstein-barr virus negative (ebv-) dlbcl.201728256900
frequent downregulation of bach2 expression in epstein-barr virus-positive diffuse large b-cell lymphoma.diffuse large b-cell lymphoma (dlbcl) is the most common b-cell lymphoma subtypes, and the epstein-barr virus (ebv)-positive subtype of dlbcl is known to exhibit a more aggressive clinical behavior than the ebv-negative one. btb and cnc homology 2 (bach2) has been highlighted as a tumor suppressor in hematopoietic malignancies; however, the role of bach2 in ebv-positive dlbcl is unclear. in the present study, bach2 expression and its significance were studied in 23 ebv-positive and 43 ebv-negati ...201728256087
the identification of up-regulated ebv-mir-bhrf1-2-5p targeting malt1 and ebv-mir-bhrf1-3 in the circulation of patients with multiple sclerosis.epstein-barr virus (ebv) is a well-documented aetiological factor for multiple sclerosis (ms). ebv encodes at least 44 micrornas (mirnas) that are readily detectable in the circulation of human. previous studies have demonstrated that ebv-encoded mirnas regulate host immune response and may serve as biomarkers for ebv-associated diseases. however, the roles of ebv mirnas in ms are still unknown. to fill the gap, we conducted a comprehensive profiling of 44 mature ebv mirnas in 30 relapsing-remit ...201728253538
the prevalence of viral agents in esophageal adenocarcinoma and barrett's esophagus: a systematic review.human papilloma virus (hpv), which may reach the esophagus through orogenital transmission, has been postulated to be associated with esophageal adenocarcinoma (eac). a systematic review of the literature investigating the prevalence of infectious agents in eac and barrett's esophagus (be) was carried out.201728252462
prolonged fever in an 8-year-old boy post-epstein-barr virus infection. 201728251742
prolonged fever in an 8-year-old boy post-epstein-barr virus infection. 201728251741
novel chemokine-based immunotoxins for potent and selective targeting of cytomegalovirus infected cells.immunotoxins as antiviral therapeutics are largely unexplored but have promising prospective due to their high selectivity potential and their unparalleled efficiency. one recent example targeted the virus-encoded g protein-coupled receptor us28 as a strategy for specific and efficient treatment of human cytomegalovirus (hcmv) infections. us28 is expressed on virus-infected cells and scavenge chemokines by rapid internalization. the chemokine-based fusion-toxin protein (ftp) consisted of a varia ...201728251165
quantitative nucleic acid amplification methods and their implications in clinical virology.recently, a number of techniques have been approved for quantification of viral nucleic acids in clinical samples. viral load (vl) tests have considerable importance in the management of patients and are widely used in routine diagnosis. in clinical virology, vl testing are important to monitor the antiviral treatment, to initiate preemptive therapy, to understand pathogenesis, and to evaluate the infectivity. these tests have now become a part of many diagnostic and treatment guidelines. consid ...201728251100
primary pulmonary lymphoepithelioma-like carcinoma on fdg pet/ct.a 69-year-old male smoker was referred to (18)f-fluorodeoxyglucose (fdg) positron emission tomography-computed tomography (pet/ct) with the indication of a suspicious solitary pulmonary nodule. fdg pet/ct determined a 2.5 × 2-cm soft-tissue lesion (suvmax, 16.2) with spiculated margins in the hilum of the right lung. a 1-cm diameter lymphadenopathy on the right hilum with an suvmax of 3.2 was also determined. transbronchial biopsy of the right hilar lymphadenopathy did not reveal any malignant f ...201728250863
plasmablastic lymphoma versus plasmablastic myeloma: an ongoing diagnostic determine the utility of clinical, morphological and phenotypical features in the differential diagnosis of plasmablastic lymphoma and myeloma with plasmablastic features.201728249941
chloroquine triggers epstein-barr virus replication through phosphorylation of kap1/trim28 in burkitt lymphoma cells.trials to reintroduce chloroquine into regions of africa where p. falciparum has regained susceptibility to chloroquine are underway. however, there are long-standing concerns about whether chloroquine increases lytic-replication of epstein-barr virus (ebv), thereby contributing to the development of endemic burkitt lymphoma. we report that chloroquine indeed drives ebv replication by linking the dna repair machinery to chromatin remodeling-mediated transcriptional repression. specifically, chlo ...201728249048
plasmablastic lymphoma: review of 60 chinese cases and prognosis analysis.plasmablastic lymphoma (pbl) is a b-cell malignancy associated with human immunodeficiency virus (hiv). pbl could also influence the hiv-negative patients. the study aimed to identify prognostic factors for survival among chinese pbl patients.201728248855
kshv-associated and ebv-associated germinotropic lymphoproliferative disorder: new findings and review of the literature.we report 2 cases of kaposi sarcoma-associated herpesvirus (kshv)-and epstein-barr virus (ebv) associated germinotropic lymphoproliferative disorder. both cases arose in patients from regions endemic for kshv, cape verde, and the democratic republic of the congo, presenting as localized lymphadenopathy. the affected lymph nodes showed colonization of the follicles by clusters of large atypical plasmablasts, but also showed regressive changes with vascular proliferation and interfollicular plasma ...201728248818
hydroa vacciniforme-like t-cell lymphoma: a further brazilian case.hydroa vacciniforme (hv)-like lymphoma is a rare, usually fatal epstein-barr virus-driven lymphoproliferative disease affecting children from asia, mexico, and south america. cutaneous manifestations imitate hv, a benign photodermatosis in which systemic symptoms are not observed, and spontaneous regression occurs later in adolescence or young adulthood. we report a case of hv-like lymphoma in a 12-year-old girl, descendent from an ancient amazon indigenous tribe that, as far as we know, represe ...201728248720
[oncogenetic factors of some herpesvirus infection agents].currently, debate continues about the etiological role of pathogens, in particular viruses, in the development of bladder tumors, their impact on the rate of recurrence and invasive and metastatic forms. the literature has reported some evidence for oncomodulating effect of cytomegalovirus in glioblastomas and bowel tumors. there is the possible role of herpes simplex virus (hsv) type 2 as carcinogenesis cofactor that initiates the development of dysplasia and maintains it in a stable condition. ...201628247637
update from the 4th edition of the world health organization classification of head and neck tumours: what is new in the 2017 who blue book for tumors and tumor-like lesions of the neck and lymph nodes.the world health organization (who) 2017 classification of head and neck tumors ("blue book") will now include a new chapter on tumors and tumor-like lesions of the neck and lymph nodes, which was not included in the previous edition. tumors and tumor-like lesions, including a variety of cysts and metastases, can arise in any component in the neck, including soft tissue, lymph nodes, and developmental remnants. the pathology and clinical features of metastatic carcinoma of unknown primary in the ...201728247228
epstein-barr virus lytic infection promotes activation of toll-like receptor 8 innate immune response in systemic sclerosis monocytes.monocytes/macrophages are activated in several autoimmune diseases, including systemic sclerosis (scleroderma; ssc), with increased expression of interferon (ifn)-regulatory genes and inflammatory cytokines, suggesting dysregulation of the innate immune response in autoimmunity. in this study, we investigated whether the lytic form of epstein-barr virus (ebv) infection (infectious ebv) is present in scleroderma monocytes and contributes to their activation in ssc.201728245863
analysis of the cross-talk of epstein-barr virus-infected b cells with t cells in the marmoset.despite the well-known association of epstein-barr virus (ebv), a lymphocryptovirus (lcv), with multiple sclerosis, a clear pathogenic role for disease progression has not been established. the translationally relevant experimental autoimmune encephalomyelitis (eae) model in marmoset monkeys revealed that lcv-infected b cells have a central pathogenic role in the activation of t cells that drive eae progression. we hypothesized that lcv-infected b cells induce t-cell functions relevant for eae p ...201728243437
the epstein-barr virus-encoded mir-bart22 targets map3k5 to promote host cell proliferative and invasive abilities in nasopharyngeal carcinoma.mir-bart22, a new discovered epstein-barr virus (ebv) mirna, is abundant in nasopharyngeal carcinoma (npc). it has been reported that mir-bart22 promoted the tumor development by down-modulating ebv lmp2 expression to evade the host immune response. but its cell target genes have still been obscure. we have reported an inverse correlation between the bart-22 and map3k5 protein expression in npc tissues and npc cell lines. meanwhile, map3k5 protein expression level was significantly decreased in ...201728243335
neurons derived from patients with bipolar disorder divide into intrinsically different sub-populations of neurons, predicting the patients' responsiveness to lithium.bipolar disorder (bd) is a progressive psychiatric disorder with more than 3% prevalence worldwide. affected individuals experience recurrent episodes of depression and mania, disrupting normal life and increasing the risk of suicide greatly. the complexity and genetic heterogeneity of psychiatric disorders have challenged the development of animal and cellular models. we recently reported that hippocampal dentate gyrus (dg) neurons differentiated from induced pluripotent stem cell (ipsc)-derive ...201728242870
targeting the programmed death-1 pathway in lymphoid neoplasms.programmed death-1 (pd-1) is a co-inhibitory molecule and is seen in cd4+ and cd8+ t cells. upon binding to its ligands, programmed death ligand-1 (pd-l1) and -2 (pd-l2), pd-1 negatively regulates interleukin 2 (il-2) production and t cell proliferation. activated effector t-cells, which kill cancer cells, can be affected by pd-1 signaling in some lymphoid neoplasm that express pd-l1 or pd-l2. pd-l1 expression in tumor cells can be induced by extrinsic signal (i.e. interferon gamma) or intrinsic ...201728242522
anaemia in hospitalised preschool children from a rural area in mozambique: a case control study in search for aetiological agents.young children bear the world's highest prevalence of anaemia, the majority of which is of multifactorial aetiology, which in turn hampers its successful prevention. even moderate degrees of anaemia are associated with increased mortality and morbidity. despite this evidence, there is a lack of effective preventive programs and absence of consensus in the safety of iron supplementation in malaria areas, which reflects the poor understanding of the contribution of different aetiologies to anaemia ...201728241813
pulmonary nodules in a kidney transplant recipient. 201728240826
oncogenic s1p signalling in ebv-associated nasopharyngeal carcinoma activates akt and promotes cell migration through s1p receptor 3.undifferentiated nasopharyngeal carcinoma (npc) is a cancer with high metastatic potential that is consistently associated with epstein-barr virus (ebv) infection. in this study, we have investigated the functional contribution of sphingosine-1-phosphate (s1p) signalling to the pathogenesis of npc. we show that ebv infection or ectopic expression of the ebv-encoded latent genes (ebna1, lmp1, and lmp2a) can up-regulate sphingosine kinase 1 (sphk1), the key enzyme that produces s1p, in npc cell li ...201728240350
hla-dq β1 alleles associated with epstein-barr virus (ebv) infectivity and ebv gp42 binding to cells.epstein-barr virus (ebv) infects b cells and ~95% of adults are infected. ebv glycoprotein gp42 is essential for entry of virus into b cells. ebv gp42 binds to the β1 chain of hla-dq, -dr, and -dp on b cells, and uses these molecules for infection. to investigate if certain hla-dq alleles are associated with ebv seronegativity, we recruited ~3,300 healthy adult blood donors, identified 106 ebv-seronegative individuals, and randomly selected a control group of ebv-seropositive donors from the don ...201728239644
ebv early lytic protein bfrf1 alters emerin distribution and post-translational modification.the nuclear envelope (ne), a structural element of fundamental importance for the cell, is the first barrier that meets a virus in the early stages of viral maturation. therefore, in order to allow the passage of nucleocapsids, viruses are known to modulate the architecture of the nuclear membrane to permit a proficient viral infection. epstein-barr virus (ebv), a pathogen from herpesvirus family, possesses two well conserved proteins, bfrf1 and bflf2, which together form the heterodimeric nucle ...201728238874
corrigendum to "niclosamide inhibits lytic replication of epstein-barr virus by disrupting mtor activation" [(antivir. res.) 138 (2017) 68-78]. 201728235454
amoxicillin-induced generalized eruption associated with epstein-barr virus reactivation in pregnancy. 201728233294
c-myc dysregulation is a co-transforming event for nf-κb activated b-cells.while c-myc dysregulation is constantly associated with highly proliferating b-cell tumors, nf-κb addiction is found in indolent lymphomas as well as diffuse large b-cell lymphomas either with an activated b-cell like phenotype or associated with epstein-barr virus. we raised the question of the effect of c-myc in b-cells with nf-κb activated by three different inducers: epstein-barr virus-latency iii program, tlr-9 and cd40. induction of c-myc overexpression increased proliferation of epstein-b ...201728232371
reappraisal of ebv in diffuse large b-cell lymphoma (dlbcl): comparative analysis between ebv-positive and -negative dlbcl with ebv-positive bystander cells.epstein-barr virus (ebv)-positive diffuse large b-cell lymphoma (dlbcl), not otherwise specified (nos) is defined as monoclonal ebv(+) b-cell proliferation affecting patients without any known immunosuppression. non-neoplastic ebv(+) cells proliferating in or adjacent to ebv(-) dlbcl were reported recently, but their clinical significance is unclear. thus, we investigated the prognostic impact of ebv(+) cells in dlbcl.201728231401
developing a multi-component immune model for evaluating the risk of respiratory illness in athletes.clinical and laboratory identification of the underlying risk of respiratory illness in athletes has proved problematic. the aim of this study was to determine whether clinical data, combined with immune responses to standardised exercise protocols and genetic cytokine polymorphism status, could identify the risk of respiratory illness (symptoms) in a cohort of highly-trained athletes. male endurance athletes (n=16; vo2max 66.5 ± 5.1 underwent a clinical evaluation of known risk f ...201728230530
variation in risk and outcomes of epstein-barr virus-associated breast cancer by epidemiologic characteristics and virus detection strategies: an exploratory study.a relationship of epstein-barr virus (ebv) and breast cancer etiology and outcome may have clinical utility and potential to enhance understanding of tumor biology. research to date has yielded variable results, likely reflecting differing virus detection assays and unaddressed epidemiologic heterogeneity across studies.201728229344
the swi/snf chromatin regulator, brg1, modulates the transcriptional regulatory activity of the ebv dna polymerase processivity factor bmrf1.during lytic phase of epstein-barr virus (ebv), binding of the transactivator zta to the origin of lytic replication (orilyt) and the bhlf1 transcript, forming a stable rna-dna hybrid, are required to initiate viral dna replication. ebv-encoded viral dna replication proteins form complexes to amplify viral dna. bmrf1, the viral dna polymerase accessory factor, is essential for lytic dna replication and also known as a transcriptional regulator of the expression of bhlf1 and balf2 (ssdna-binding ...201728228591
crohn's disease complicated by epstein-barr virus-driven haemophagocytic lymphohistiocytosis successfully treated with rituximab.we report a case of epstein-barr virus (ebv)-driven haemophagocytic lymphohistiocytosis (hlh) in a man with crohn's disease treated with 6-mercaptopurine and adalimumab therapy who was successfully treated with rituximab therapy alone. this is the first published case in an adult patient with ebv-driven hlh in the setting of thiopurine use and inflammatory bowel disease to be successfully treated with rituximab therapy alone. here, we will discuss putative immunological mechanisms which may cont ...201728228435
anti-tumour research of recombinant bcg using bzlf1 and hgm-csf fusion genes.the random primer oligo(dt)15 was used in rt-pcr to obtain cdna from the human granulocyte macrophage colony stimulating factor (hgm-csf) gene and the epstein-barr virus (ebv) gene bzlf1. then, the sequence splicing overlap extension method was used to obtain a gcbf fusion gene containing a linker sequence that encoded the polypeptide (gly4ser)3. the gcbf fusion gene was inserted into pmv261, which was then transformed into competent e. coli dh5 alpha cells, and positive cells were selected base ...201728228322
cd117 (kit) is a useful marker in the diagnosis of plasmablastic plasma cell myeloma.plasmablastic plasma cell myeloma (ppcm) is a rare morphological presentation of multiple myeloma and can resemble plasmablastic lymphoma (pbl), an hiv related lymphoid neoplasm, morphologically and immunophenotypically. we retrospectively searched for factors that could help differentiate these two entities.201728226184
a new case of acute idiopathic frosted branch angiitis in europe.a 20-year-old male with no history of any systemic or eye disease developed loss of visual acuity in both eyes. white exudates surrounding the retinal veins from the posterior pole to the periphery, retinal edema and hemorrhages in both eyes were evident on ophthalmoscopy. fluorescein angiography showed leakage of the dye from the veins and extensive staining of the vein walls. a diagnosis of acute frosted branch angiitis was made. systemic examination revealed axillary, submandibular and inguin ...201728226129
correlations between epstein-barr virus and acute leukemia.infection with epstein-barr virus (ebv) may be correlated to the onset of acute leukemia (al). studies were performed to investigate the relationship between ebv infection and immunophenotyping of acute lymphoblastic leukemia (all) and chromosome aberrations. additionally, the effects of ebv on clinical prognosis were described. fluorescence quantitative polymerase chain reaction (fq-pcr) was used to detect ebv-dna copy numbers from bone marrow in 110 cases of patients with all, 75 cases of pati ...201728225168
global trends in nasopharyngeal cancer mortality since 1970 and predictions for 2020: focus on low-risk areas.nasopharyngeal cancer (npc) mortality shows great disparity between endemic high risk areas, where non-keratinizing carcinoma (nkc) histology is prevalent, and non-endemic low risk regions, where the keratinizing squamous cell carcinoma (kscc) type is more frequent. we used the world health organization database to calculate npc mortality trends from 1970 to 2014 in several countries worldwide. for the european union (eu), the united states (us) and japan, we also predicted trends to 2020. in 20 ...201728224615
cytokine profiles as novel diagnostic markers of epstein-barr virus-associated hemophagocytic lymphohistiocytosis in children.the aim of this study was to identify specific laboratory indices to distinguish epstein-barr virus-associated hemophagocytic lymphohistiocytosis (ebv-hlh) in children.201728222313
epstein-barr virus-specific adoptive immunotherapy for recurrent, metastatic nasopharyngeal carcinoma.early-stage and intermediate stage nasopharyngeal cancer (npc) generally carry a good prognosis, but for patients with recurrent, metastatic disease, options are limited. in the current study, the authors present a phase 1/2 study to evaluate the efficacy of epstein-barr virus (ebv)-stimulated cytotoxic t-lymphocyte (ebv-ctl) immunotherapy in this patient population.201728222215
hepatobiliary and pancreatic: epstein-barr virus-associated smooth muscle tumors: unusual cause of hepatic mass in aids patient. 201728221693
epstein-barr positive t-cell lymphoma in the ocular present two cases of rapidly growing tumors in the ocular adnexa. both tumors were epstein-barr virus (ebv) positive peripheral t-cell lymphoma.201728221481
characterization of epstein-barr virus-encoded small rna gene variations in virus associated lymphomas in northern china.epstein-barr virus (ebv)-encoded small rnas (eber1 and eber2) are highly expressed in all forms of ebv latency in ebv-associated malignancies. eber gene variations and their association with ebv-associated disease still remain poorly characterized. to investigate the patterns of eber gene variations and their roles in tumorigenesis, eber gene sequences were analyzed by nested-pcr and dna sequencing in 101 lymphomas from northern china, a non-nasopharyngeal carcinoma (npc) endemic area. in additi ...201728220325
molecular testing for gastrointestinal cancer.with recent advances in molecular diagnostic methods and targeted cancer therapies, several molecular tests have been recommended for gastric cancer (gc) and colorectal cancer (crc). microsatellite instability analysis of gastrointestinal cancers is performed to screen for lynch syndrome, predict favorable prognosis, and screen patients for immunotherapy. the epidermal growth factor receptor (egfr) tyrosine kinase inhibitor has been approved in metastatic crcs with wildtype ras (kras and nras ex ...201728219002
prognostic role of plasma epstein-barr virus dna load for nasopharyngeal carcinoma: a meta-analysis.predicting prognosis and treatment outcomes for patients with for nasopharyngeal carcinoma (npc) has been difficult due to the heterogeneous nature of the disease this study aimed to evaluate pretreatment copy number of plasma epstein-barr virus (ebv) dna as an outcome marker for survival in npc.201728218577
coexistence of age-related ebv-associated follicular hyperplasia and large b-cell ebv+ lymphoma of the elderly. two distinct features of the same t-cell dysfunction related to senescence?age-related ebv-associated lymphoproliferative disorders form a new clinicopathological group. until recently, this group was associated with diffuse large b-cell lymphoma (dlbcl), characterised by an aggressive clinical presentation and a poor prognosis. recent findings in western caucasian patients, however, suggest that this entity covers a wide spectrum of diseases, ranging from reactive follicular hyperplasia (hr) to dlbcl. we report the case of an 85-year-old caucasian man showing lymphade ...201728215641
lymphoepithelioma-like carcinoma of cervix: cytological features on conventional cervical smear.lymphoepithelioma-like carcinoma (lelc) is a rare neoplasm of the cervix. the importance of distinguishing this undifferentiated carcinoma with a predominant lymphocytic infiltrate lies in the fact that despite being poorly differentiated they have a better prognosis. the diagnosis however becomes more challenging when the pathologist is provided with a small cervical biopsy or a papanicolaou smear. while the reports describing histology and their relation to epstein-barr virus (ebv) are many, t ...201728211234
how we treat chronic active epstein-barr virus infection.chronic active epstein-barr virus infection (caebv) is a prototype of the ebv-associated t- or nk-cell lymphoproliferative diseases, which also include hypersensitivity to mosquito bites and severe-type hydroavacciniforme. the manifestations of caebv are often self-limiting with minimum supportive care or only prednisolone and cyclosporine a with or without etoposide. however, allogeneic hematopoietic stem cell transplantation (hsct) is the only cure, without which patients with caebv die within ...201728210942
hemophagocytic lymphohistiocytosis in a patient with hodgkin lymphoma and concurrent ebv, cmv, and candida infections.hemophagocytic lymphohistiocytosis (hlh) is a syndrome characterized by immune activation and subsequent widespread organ damage. patients affected by hlh commonly develop fever, cytopenias, liver damage, neurologic manifestations, and hypercytokinemia. in this case, we describe a 60-year-old male who presented with hlh and concurrent epstein-barr virus, cytomegalovirus, and candida infections and was subsequently diagnosed with a hodgkin lymphoma. this case highlights the importance of consider ...201728210636
epstein-barr virus-induced mononucleosis as an imitator of severe preeclampsia.background in pregnancy, conditions presenting with hematologic abnormalities, transaminitis, and proteinuria pose diagnostic challenges in pregnancy. case we present the case of an 18-year-old woman, g1p0, at 33 weeks' gestation with fever of unknown cause, who developed progressively elevated liver enzymes, proteinuria, and thrombocytopenia, due to epstein-barr virus (ebv) infection. conclusion acute infection with ebv should be included in the differential diagnosis of preeclampsia with sever ...201728210518
the cumulative burden of double-stranded dna virus detection after allogeneic hct is associated with increased mortality.strategies to prevent active infection with certain double-stranded dna (dsdna) viruses after allogeneic hematopoietic cell transplantation (hct) are limited by incomplete understanding of their epidemiology and clinical impact. we retrospectively tested weekly plasma samples from allogeneic hct recipients at our center from 2007-2014. we used quantitative pcr to test for cytomegalovirus (cmv), bk polyomavirus (bkv), human herpesvirus 6b (hhv-6b), hhv-6a, adenovirus (adv), and epstein-barr virus ...201728209721
use of plasma epstein-barr virus dna monitoring as a tumor marker in follow-up of patients with nasopharyngeal carcinoma: preliminary results and report of two cases.recent studies suggest that plasma epstein-barr virus (ebv) dna may reflect tumor burden in patients with nasopharyngeal cancer. a prospective study was initiated to investigate this correlation in 125 patients (34 pretreatment [group a], 78 in remission [group b] and 13 relapsed [group c]) and 19 healthy controls. in group a, ebv dna was detected in plasma samples of 24 (70%) patients. in group b, ebv dna was detected in 7 patients (range 77-13,731 copies/ml) and further imaging in all but one ...201728207133
clinical application of early gastric carcinoma with lymphoid stroma based on lymph node metastasis status.gastric carcinoma with lymphoid stroma (gcls) is characterized by undifferentiated carcinoma mixed with prominent lymphoid infiltration. gcls has unique clinicopathological features and a better prognosis compared to other types of gastric cancer. we analyzed the clinicopathological features of early gcls in relation to lymph node metastasis (lnm).201728205059
prevalence and significance of plasma epstein-barr virus dna level in nasopharyngeal carcinoma. 201728204596
dual infection with hepatitis b and epstein-barr virus presenting with severe jaundice, coagulopathy, and hepatitis b virus chronicity outcome.background hepatitis b virus (hbv) has been reported as a coinfection with hepatitis c virus (hcv), hepatitis d virus (hdv), cytomegalovirus (cmv), and human immunodeficiency virus (hiv). case report a 34-year-old female presented to our clinic with epigastric pain and severe acute hepatitis manifested as jaundice associated with hyperbilirubinemia, elevated transaminases, and coagulopathy. the patient was diagnosed with acute hbv with epstein-barr virus (ebv) coinfection leading to subsequent c ...201728202897
long term treatment with abatacept or tocilizumab does not increase epstein-barr virus load in patients with rheumatoid arthritis - a three years retrospective study.epstein-barr virus (ebv) is a widely disseminated lymphotropic herpes virus implicated in benign and malignant disorders. in transplant patients, immunosuppressive drugs (cyclosporine) diminish control of ebv replication, potentially leading to lymphoproliferative disorders (lpd). rheumatoid arthritis (ra) patients have impaired control of ebv infection and have ebv load ten times higher than controls. as post transplant patients, patients with ra have increased risk of developing lymphomas. imm ...201728199343
crispr-cas9-mediated disruption of pd-1 on human t cells for adoptive cellular therapies of ebv positive gastric cancer.the successful use of immune cell checkpoint inhibitors pd-1 and pd-l1, over the past 5 y has raised the concern of using immunotherapy to treat various cancers. epstein-barr virus-associated gastric cancer (ebvagc) exhibits high infiltration of lymphocytes and high amplification of immune-related genes including pd-l1 as distinguished from epstein-barr virus-non-associated gastric cancer (ebvngc). here, we presume that this pd-1/pd-l1 pathway may hinder the efficacy of adoptive t cell therapy t ...201728197365
defective t-cell control of epstein-barr virus infection in multiple sclerosis.mounting evidence indicates that infection with epstein-barr virus (ebv) has a major role in the pathogenesis of multiple sclerosis (ms). defective elimination of ebv-infected b cells by cd8(+) t cells might cause ms by allowing ebv-infected autoreactive b cells to accumulate in the brain. here we undertake a comprehensive analysis of the t-cell response to ebv in ms, using flow cytometry and intracellular ifn-γ staining to measure t-cell responses to ebv-infected autologous lymphoblastoid cell ...201728197337
the c-terminus of epstein-barr virus brrf2 is required for its proper localization and efficient virus production.epstein-barr virus (ebv) is a human gammaherpesvirus associated with several malignancies. we reported previously that an ebv lytic gene product brrf2 is involved in the maturation of progeny virus. to analyze the domain(s) needed for efficient production of progeny, we prepared a series of deletion mutants and found two functional domains in the n- and c-terminal regions by complementation assays. immunofluorescence analyses revealed that brrf2 lacking the c-terminal region demonstrated aberran ...201728197146
coxsackie myocarditis and hepatitis with reactivated epstein-bar virus (ebv): a case report.background myocarditis, defined as inflammation of myocardial tissue of the heart, is an uncommon cardiac presentation and is due to a variety of causes. it affects 1% of the us population, 50% of which is caused by coxsackie b virus. cardiac tissue is the prime target, and destruction of myocardium results in cardiac failure with fluid overload. case report our patient was a 57-year-old woman with fever, headache, neck pain, and generalized malaise. her white blood cell count was 13×10³ cells/m ...201728196967
ebv-induced lymphoproliferative disorders in rheumatic patients: a systematic review of the literature.epstein-barr virus (ebv) is involved in the pathogenesis of approximately 40% of lymphoproliferative disorders (lpds) arising in patients receiving immunosuppressive treatment (ist) for rheumatic diseases, but data from large cohorts are still not available. we aimed to identify clinicopathological features, management and outcome of this condition.201728196776
fibrin-associated ebv-positive large b-cell lymphoma: an indolent neoplasm with features distinct from diffuse large b-cell lymphoma associated with chronic inflammation.incidental cases of localized fibrin-associated epstein-barr virus (ebv)+ large b-cell proliferations have been described at unusual anatomic sites and have been included in the category of diffuse large b-cell lymphoma associated with chronic inflammation (dlbcl-ci) in the who classification. we describe 12 cases and review the literature to define their clinicopathologic spectrum and compare features with typical cases of dlbcl-ci. median age was 55.5 years with a m:f ratio of 3. in all 12 cas ...201728195879
metastatic nasopharyngeal carcinoma presenting as an isolated breast mass: a diagnostic pitfall and a review of literature.metastases to breast are much rarer than primary breast tumors. we now present a case of 45-year-old female, who presented with an isolated breast mass. a positron emission tomography-computed tomography (pet-ct) done revealed hypermetabolic right breast nodules, soft tissue deposits, and multiple nodal involvement. the biopsy from the breast and axillary lymph node showed dense lymphoid infiltrate and was interpreted initially as granulomatous inflammation. however, the lumps were hard and susp ...201728195109
a paper-based platform for detection of viral rna.viral detection presents a host of challenges for even the most sensitive analytical techniques, and the complexity of common detection platforms typically preclude portability. with these considerations in mind, we designed a paper microzone plate-based virus detection system for the detection of viral genetic material that can be performed with simple instruments. the sensing system can detect viral cdna reverse-transcribed from total rna extraction by utilizing a biotinylated capture probe an ...201728194453
acute acalculous cholecystitis: a rare presentation of primary epstein-barr virus infection in adults-case report and review of the literature.primary epstein-barr virus (ebv) infection is almost always a self-limited disease characterized by sore throat, fever, and lymphadenopathy. hepatic involvement is usually characterized by mild elevations of aminotransferases and resolves spontaneously. although isolated gallbladder wall thickness has been reported in these patients, acute acalculous cholecystitis is an atypical presentation of primary ebv infection. we presented a young women admitted with a 10-day history of fever, nausea, mal ...201728194287
nk/t cell lymphoma: a tertiary centre experience.extranodal nk/t-cell lymphoma (enktl), nasal type, is a rare type of non-hodgkin lymphoma that is commonly seen in east asian countries and is associated with epstein-barr virus infection. this is a retrospective study where we describe nine cases of enktl; nasal type diagnosed and treated at our center over a period of 2 years. these cases were analyzed retrospectively for clinical presentation (age, sex, site of involvement), immunophenotype, treatment, response and toxicity profile. sino-nasa ...201728194059
a case report of primary nasal natural killer (nk)/t-cell lymphoma in an african american patient presenting with hemophagocytic syndrome.background extranodal natural killer/t-cell lymphoma, nasal type (enktcl) is generally an aggressive and rare non-hodgkin lymphoma. it is most common in east asians, native americans, and south americans, but is rarely reported in blacks. case report a 55-year-old african american male born in grenada presented with a left nostril mass with facial swelling and biopsy subsequently confirmed a diagnosis of extranodal nk/t-cell lymphoma, nasal type (enktcl). immunochemistry was positive for cd2, cy ...201728193996
strong anti-epstein barr virus (ebv) or cytomegalovirus (cmv) cellular immune responses predict survival and a favourable response to anti-tuberculosis therapy.intact immune responses to cytomegalovirus (cmv) and epstein-barr virus (ebv) represent a biologically and clinically relevant correlate of 'immunological fitness' in humans. however, there is a lack of knowledge concerning anti-ebv or anti-cmv responses in patients with pulmonary tuberculosis (tb), in whom aberrant immune responses may promote progression of clinical disease.201728193504
asymptomatic epstein-barr virus shedding in the urine of kidney transplant recipients: case reports and review of the literature.epstein-barr virus (ebv) is associated with a wide range of malignancies and complications like post-transplant lymphoproliferative disorder (ptld). to suppress active ebv infection in transplant recipients, who are at a heightened risk of developing ptld, ebv dnaemia monitoring has been recommended. quantitative multiplex real time polymerase chain reaction (qmrtpcr) offered the advantage of detection of more than one target in the same sample. we present four cases of kidney transplant recipie ...201628191298
leishmania infantum and epstein-barr virus co-infection in a patient with hemophagocytosis.the authors describe a rare case of a 27- year old previously healthy male presenting with high grade fever, pancytopenia, hepatosplenomegaly, high levels of ferritin and triglyceride, suggesting a diagnosis of hemophagocytic lymphohistiocytosis (hlh) syndrome. other investigations showed a positive leishmania infantum serology and high epstein-barr virus (ebv) viremia. the diagnosis of a visceral leishmaniasis was confirmed by bone morrow biopsy, which showed leishman-donovan bodies and evidenc ...201628191297
epstein-barr virus in the pathogenesis of oral cancers.epstein-barr virus (ebv) is a ubiquitous gammaherpesvirus that establishes a lifelong persistent infection in the oral cavity and is intermittently shed in the saliva. ebv exhibits a biphasic life cycle, supported by its dual tropism for b lymphocytes and epithelial cells, which allows the virus to be transmitted within oral lymphoid tissues. while infection is often benign, ebv is associated with a number of lymphomas and carcinomas that arise in the oral cavity and at other anatomical sites. i ...201728190296
mutation analysis of tumor necrosis factor alpha-induced protein 3 gene in hodgkin lymphoma.survival and proliferation of hodgkin and reed-sternberg (hrs) cells, the malignant cells of classical hodgkin lymphoma (chl), are dependent on constitutive activation of nuclear factor kb (nf-κb). a20, encoded by tnf alpha-induced protein 3 (tnfaip3), one of the inhibitors of nf-kb, was found to be inactivated by deletions and/or point mutations in chl.201728189285
alice in wonderland syndrome: a real life version of lewis carroll's novel.alice in wonderland syndrome was originally coined by dr. john todd in 1955. the syndrome is named after the sensations experienced by the character alice in lewis carroll's novel alice's adventures in wonderland. alice in wonderland syndrome consists of metamorphopsia (seeing something in a distorted fashion), bizarre distortions of their body image, and bizarre perceptual distortions of form, size, movement or color. additionally, patients with alice in wonderland syndrome can experience audit ...201728189272
pd1 blockade with pembrolizumab is highly effective in relapsed or refractory nk/t-cell lymphoma failing l-asparaginase.natural killer (nk)/t-cell lymphomas failing l-asparaginse-regimens have no known salvage and are almost invariably fatal. seven male nk/t-cell lymphoma patients (age: 49, 31-68, years) failing 2 (1-5) regimens (including l-asparaginase-regimens, and allogeneic hematopoietic stem cell transplantation, hsct, in 2 cases), were treated with the anti-programmed-death-1 (pd1) antibody pembrolizumab. all patients responded, according to various clinical, radiologic (positron emission tomography), morp ...201728188133
epstein-barr virus particles induce centrosome amplification and chromosomal instability.infections with epstein-barr virus (ebv) are associated with cancer development, and ebv lytic replication (the process that generates virus progeny) is a strong risk factor for some cancer types. here we report that ebv infection of b-lymphocytes (in vitro and in a mouse model) leads to an increased rate of centrosome amplification, associated with chromosomal instability. this effect can be reproduced with virus-like particles devoid of ebv dna, but not with defective virus-like particles that ...201728186092
epstein-barr virus-associated gastric cancer reveals intratumoral heterogeneity of pik3ca mutations. 201728184407
serum proteomic-based analysis identifying autoantibodies against prdx2 and prdx3 as potential diagnostic biomarkers in nasopharyngeal carcinoma.nasopharyngeal carcinoma (npc) is a major head and neck cancer with high occurrence in southeast asia and southern china. we aimed to identify autoantibodies that may contribute to early detection of npc.201728184180
imaging the modulation of adenoviral kinetics and biodistribution for cancer gene therapy.see page 841 to explore systemic utilization of epstein-barr virus (ebv)-specific transcriptionally targeted adenoviruses, three vectors were constructed to examine kinetics, specificity, and biodistribution: adv.orip.luc, expressing luciferase under ebv-specific control; adv.sv40luc, expressing luciferase constitutively; and adv.orip.e1a.orip.luc, a conditionally replicating adenovirus, expressing both luciferase and e1a. bioluminescence imaging (bli) was conducted on tumor-bearing severe combi ...200728182894
next-generation sequencing of mirnas in clinical samples of epstein-barr virus-associated b-cell lymphomas.epstein-barr virus (ebv) encodes 49 micrornas (mirnas) in the bart and bhrf1 regions of its genome. although expression profiles of ebv-encoded mirnas have been reported for ebv-positive cell lines and nasopharyngeal carcinoma, to date there is little information about total mirna expression, including cellular and viral mirnas, in the primary tumors of ebv-associated b-lymphoproliferative disorders. in this study, next-generation sequencing and quantitative real-time reverse transcription-pcr w ...201728181423
the prognostic value and pathobiological significance of glasgow microenvironment score in gastric evaluate the prognostic value and pathobiological significance of glasgow microenvironment score (gms), a parameter based on tumor stroma percentage and inflammatory cell infiltration, in gastric cancer.201728180998
"hemophagocytic lymphohistiocytosis after ebv reactivation and ibrutinib treatment in relapsed/refractory chronic lymphocytic leukemia".hemophagocytic lymphohistiocytosis (hlh) is a rare syndrome characterized by ineffective t-cell and nk response. we report the clinical course of a patient with relapsed cll who developed acute symptoms soon after starting ibrutinib. hyperpyrexia, splenomegaly, hyperferritinemia, hypertriglyceridemia, cytopenias, and a typical cytokine pattern, i.e. high interleukin (il)-6, il10 and il18, were consistent with a diagnosis of hlh. coexistent epstein barr virus reactivation was documented. ibrutini ...201728180067
differentiation-dependent lmp1 expression is required for efficient lytic epstein-barr virus reactivation in epithelial cells.epstein-barr virus (ebv)-associated diseases of epithelial cells, including tumors that have latent infection, such as nasopharyngeal carcinoma (npc), and oral hairy leukoplakia (ohl) lesions that have lytic infection, frequently express the viral latent membrane protein 1 (lmp1). in lytically infected cells, lmp1 expression is activated by the brlf1 (r) immediate early (ie) protein. however, the mechanisms by which lmp1 expression is normally regulated in epithelial cells remain poorly understo ...201728179525
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