activation of latent epstein-barr virus by antibody to human igm. 1978213727
[recent developments in the field of infectious diseases].a résumé is presented of recent developments in the field of infectious diseases of particular interest of practising physicians. examples are given to illustrate recent discoveries concerning the frequent misuse of antibiotics, the problem of infections developing in hospitalized patients, the biology of microbial infection, the etiology of well-known illnesses, new infectious diseases, and the development of vaccines. it is evident that the rate at which new and important information is eviden ...1978213830
induction of nuclear antigen (ebna) synthesis without transformation in human cord lymphocytes by p3hr-1 strain of epstein-barr virus. 1978213884
detection of b-lymphocyte (b-cell)-associated antigens on human leukemic lymphocytes. masking of membrane indirect immunofluorescent technic has been used for rabbit antisera (anti-ep) that demonstrated complement-dependent cytotoxicity against human leukemic lymphocytes but not against normal blood lymphocytes. with the immunofluorescent technic, the antisera were found to react with 2--23% of normal blood lymphocytes. simultaneous staining of normal cells with anti-ep and for surface immunoglobulins (sig) on bone-marrow-derived b-cells showed that the proportions of stained cells were similar t ...1978213964
grand rounds--albany medical center. epstein-barr virus. the ravager. 1978214009
tumor viruses and human cancer. ii. dna tumor viruses. 1978214057
ebna antibodies are more useful than complement fixing antibodies in monitoring infectious mononucleosis patients.thirty-six patients with infectious mononucleosis (im) were followed prospectively for both anti-epstein-barr virus nuclear antigen (ebna) and complement-fixing antibodies using the soluble ebv antigen (cf/s). discrepancies in time between the two reactivities were found, due to the differences in sensitivity of the tests. it is suggested that, in clinical virology laboratories, ebna and not cf/s tests be used.1978214180
biology of the human malignant lymphomas. iv. functional characterization of ten diffuse histiocytic lymphoma cell lines.ten consecutive diffuse histiocytic lymphoma (dhl) cell lines established in our laboratory were studied for the presence of epstein-barr virus (ebv) genomes, lysozyme, nonspecific esterase and other cytochemical reactions, phagocytic activity, cytoplasmic immunoglobulin light and heavy chains, and surface receptors to sheep erythrocytes, complement, and the fc fragment of immunoglobulin. in agreement with previous studies performed on biopsy specimens, our results indicate that the diffuse hist ...1978214220
stimulation of human peripheral blood lymphocytes by autologous ebv-infected b cells. 1978214248
long-term t-cell-mediated immunity to epstein-barr virus in man. i. complete regression of virus-induced transformation in cultures of seropositive donor leukocytes.peripheral blood mononuclear cells from donors of known serological status with respect to eb virus were exposed to the virus in vitro and then cultured at various cell concentrations. all cultures from nine seronegative adult and 12 foetal donors gave rise to cell lines following subculture 4 weeks post infection. in contrast, seropositive donor cultures seeded at the higher cell concentrations developed foci of proliferating ebna-positive cells within the first 1--2 weeks but thereafter regres ...1978214405
malignant lymphoma in four of five one family four out of five siblings were affected with malignant lymphoma and hd has been diagnosed in two other relatives. hla-haplotypes were identical for the three diseased siblings who were so studied, whereas for the unaffected one they were different.1978214406
in vitro effects of epstein-barr virus on peripheral blood mononuclear cells from patients with rheumatoid arthritis and normal subjects.peripheral blood mononuclear cells from 10 patients with rheumatoid arthritis and 9 control subjects were cultured in vitro for 30 days with and without infection by epstein-barr virus. all cultures showed polyclonal stimulation of b cells as indicated by rising levels of igm in the culture supernates, reaching maximal at 18-24 days, and with no quantitative or kinetic difference between the ra and control cells. igm anti-igg was also produced in both groups and maximally at 18-24 days, but in g ...1978214511
dna of epstein-barr virus. iv. linkage map of restriction enzyme fragments of the b95-8 and w91 strains of epstein-barr virus.the arrangement of ecori, hsu i, and sal i restriction enzyme sites in the dna of the b95-8 and w91 isolates of epstein-barr virus (ebv) has been determined from the size of the single-enzyme-cleaved fragments and from blot hybridizations that identify which fragments cut from the dna with one enzyme contain nucleotide sequences in common with fragments cut from the dna with a second enzyme. the dna of the b95-8 isolate was the prototype for this study. the data indicate that (i) approximately ...1978214576
kawasaki-like disease in a young adult. association with primary epstein-barr virus infection. 1979214590
thoughts on kawasaki disease etiology. 1979214591
electron microscopic studies of tumor viruses. ii. entry and uncoating of epstein-barr virus.entry of epstein-barr virus into human lymphoblastoid cells (daudi cells) was studied by electron microscopy. at the site of viral attachment, two distinct interactions conducive to penetration of the virus occurred between the viral envelope and cell membrane, namely, (i) simultaneous dissolution of both the envelope and cell membrane, presumably resulting in passage of viral capsids into the cytoplasm and (ii) dissolution confined to the cell membrane with resulting penetration of enveloped vi ...1978214669
epstein-barr virus early antigen titer by immunofluorescence in microplates. a new semi-automated method based on microtiter system.for the titration of antibodies directed against epstein-barr virus early antigen (ebv-ea) we describe a method in microplates with the microtiter system (cooke engineering co., alexandria, va). using this technique, which allows rapid epidemiological investigations, we have titered anti-ebv-ea antibodies in different groups of patients and controls, and particularly in group which had recent contact with infectious mononucleosis. in this group the percentage of individuals having antibodies dir ...1978214682
complete heart block in epstein-barr myocarditis. 1978214746
antigenic content of an in vitro cellular immunity reactive cell surface extract of raji cells.antigenic content of a raji cell surface extract (ts) was studied by sequential abosrption of rabbit antiserum to this extract with different cells. anti-ts was first allowed to react exhaustively with human blood cells followed by hela cells and human diploid fibroblasts. the residual reactivity was studied by stepwise absorption with an ebv-negative b lymphoblastoid cell line, bjab, and then with b1--19, a clone derived from ebv transformed bjab cells. these processes delineated three kinds of ...1978215106
a study of epstein barr virus receptor activity in cell free extracts of human lymphoblastoid cells.epstein barr virus (ebv) receptor activity in cell free extracts is operationally defined as one which causes a reduction in the effective concentration of the early antigen inducing particles of an ebv preparation when the latter is preincubated with the extract before infection. such activity was detected in the surface extract of raji cells and to a lesser extent in that of bjab cells, both of which are b lymphoblastoid cells that are susceptible to infection with ebv. receptor activity was n ...1978215107
histological types of nasopharyngeal carcinoma in an intermediate risk area.between april and december 1974, 485 cases of npc were seen at the salah azaïz institute in tunis. a histological study was carried out according to a classification that comprises four types of tumour:well-differentiated squamous-cell carcinoma, poorly-differentiated squamous-cell carcinoma, 'nasopharyngeal' type carcinoma and an anaplastic type. some correlation between these histological types and clinical, epidemiological, virological and prognostic features was found.1978215511
clinical aspects and natural history of nasopharyngeal carcinoma in western europe. 1978215512
descriptive and analytical epidemiology of nasopharyngeal cancer.information concerning the descriptive and analytical epidemiology of npc that has been reported mainly since the first international symposium on the subject in singapore in 1964 are reviewed. npc is rare in most countries in the world, with an age-adjusted incidence rate of less than 1 per 100,000, and the incidence rate is twice as high in males as in females. chinese of southern origin have a uniquely high risk, the incidence rates per 100,000 being 10--20 in males and 5--10 in females. the ...1978215514
histopathological types of nasopharyngeal carcinoma in a low-risk area: japan.npc in japan was studied histopathologically by examining 816 npcs from among 3 338 biopsies from patients with tumours of the nasopharynx and other parts of the upper respiratory system. in addition, a comparative study on npc among taiwanese and an immunoserological study were carried out. when comparing histological types of malignant tumours of the upper respiratory tract, poorly-differentiated squamous-cell carcinoma predominated only in those of the nasopharynx. malignant nasopharyngeal tu ...1978215515
histological typing of nasopharyngeal carcinoma.there is little agreement about correlations between histological type and the epidemiological and biological characteristics of npc. this is largely due to variations in terminology and diagnostic criteria. the use of the terminology and definitions proposed by who would facilitate and improve international comparisons.1978215516
epstein-barr virus--discovery, properties and relationship to nasopharyngeal carcinoma. 1978215517
morphological transformation of nasopharyngeal epithelial cells in vitro by epstein-barr virus from b95-8 cells.tissue fragments of fresh biopsy specimens from the non-neoplastic np mucosa of 20 subjects, from the tumours of 10 npc patients, from the mucosa of freshly removed tonsils from nine subjects and from the primary lesions of seven patients with malignancies of the upper respiratory or alimentary tract other than npc were infected with ebv derived from b95-8 cells. a significantly higher frequency of growth stimulation and a greater mean growth ratio between infected and uninfected fragments from ...1978215518
epstein-barr virus-regulation studies on somatic-cell hybrids derived from the fusion of burkitt's lymphoma and nasopharyngeal carcinoma cells with human or mouse partners. 1978215519
epstein-barr virus-epithelial cell interaction and its implication in the etiology of nasopharyngeal carcinoma.the etiological role of ebv in npc is still a matter for debate. a major question is how the viral dna becomes associated with the carcinoma cell, since only b lymphocytes are at present known to have receptors to ebv. the following hypotheses are proposed: (1) the epithelial cells of the nasopharynx have ebv receptors in vivo; transformation of the epithelial cell thus results from a direct interaction between ebv and this cell type. the epithelial cells of the nasopharynx are not permissive fo ...1978215520
early and late components of epstein-barr virus-associated membrane antigen in superinfected daudi cells and their reactivity with sera from nasopharyngeal carcinoma patients.investigations were carried out on newly synthesized ma, vca and ea in daudi cells superinfected with the p3hr-1 strain of ebv and treated with trypsin to remove previously adsorbed ma-positive material from the cell surface. synthesis of ma, vca and ea was completely blocked by puromycin. a marked reduction in the frequency of ma-positive cells was observed in the infected cell cultures in the presence of either ara-c or pa, but a fraction of the ma-positive cells was insensitive to the inhibit ...1978215521
heterogeneity of epstein-barr virus derived from p3hr-1 cells.infection of cells of the ebv-free human b-lymphoma lines bjab and ramos resulted in conversion of these cells to ebv-genome carriers expressing ebna. ebv isolates from p3hr-1 cells induced a heterogeneous ebna pattern: both a faintly granular pattern and brilliant ebna-expression were observed. the two types of ebna-expressing cells could be separated upon cloning. brilliantly ebna-expressing cells always segregated varying percentages of ebna-negative cells. an ebna-negative subclone derived f ...1978215522
expression of latent epstein-barr virus genomes in human epithelial/burkitt's lymphoblastoid hybrid cells.expression of latent epstein-barr virus genomes in somatic-cell hybrids of burkitt's lymphoblastoid cells has been studied. treatment of the hybrid cells, d98/raji and d98/hr-1, with iudr induced the formation of ea and vca and replication of virus dna, whereas the same treatment of raji cells induced only the formation of ea. the patterns of transcription of virus genomes in these three cell lines were, however, very similar: 25% without treatment with iudr, 30% immediately after the treatment ...1978215523
interaction between epstein-barr virus and type-c virus in human cells.the interaction between ebv and type-c viruses was studied in our fvnc experimental system, in which ebv and type-c viral genomes are contained in each cell. the data indicate that the human lymphoid fvnc cells are sensitive to both ebv and type-c virus exposure, showing high frequencies of induction of both repressed viral genomes. the two different viral genomes may be associated with different chromosomes in individual cells.1978215524
epstein-barr virus-related serology in nasopharyngeal carcinoma and controls. 1978215525
clinical evaluation of ebv serology in american patients with nasopharyngeal carcinoma.there is now extensive immunological, biological and biochemical evidence to support a possible etiological relationship between ebv and npc in patients from different geographical locations. besides providing information on the question of etiology, the results from immunological investigations suggest that antibodies to some of the ebv-associated antigens might also be of clinical importance in the diagnosis and prognosis of npc. to determine the possible clinical application of ebv serology t ...1978215526
the genetic and antigenic basis for the iga antibody response to epstein-barr viral capsid antigen. 1978215527
presence of epstein-barr virus specific iga in saliva of nasopharyngeal carcinoma patients: their activity, origin and possible clinical value. 1978215528
differences in ebv antibody titres of patients with nasopharyngeal carcinoma originating from high, intermediate and low incidence order to assess the differences in serological reactivities of npc patients from high, intermediate and low incidence areas, 288 npc sera from hong kong, singapore, tunis, east africa, paris and los angeles, together with sera from patients with ear, nose and throat tumours other than npc and with those from normal individuals from the same areas, were tested 'blind' with the same batches of antigen. important differences (up to 3-fold) in the gmts of antibodies directed against vca, ea and e ...1978215529
cell-mediated immunity, epstein-barr virus and nasopharyngeal carcinoma.cellular immunity is apparently important in host defenses against cancer. a growing body of information on cmi to ebv is here reviewed, providing a strong basis for studies of such reactivity in npc. present work suggests that cmi responses to virus- and tumour-associated antigens may have implications for etiology, diagnosis and treatment.1978215530
general immunological status of nasopharyngeal carcinoma patients in singapore.newly diagnosed npc patients were found to have impaired general t-cell functions, as determined in vivo by the mantoux test and in vitro by the pha response assay. treated remission patients were as hyporesponsiveness as newly diagnosed patients. pha hyporesponsiveness was associated with the hla profile of a2-b sin 2 and with high antibody titres to ebv-related antigens, in particular to the early antigen. impaired responses in the mantoux or pha tests were associated with poor survival. the i ...1978215531
autoantibodies (cold lymphocytotoxins, antiactin antibodies and antinuclear factors) in nasopharyngeal carcinoma patients.lta, sma and ana were found at higher frequencies in sera from npc patients than in those from matched controls. the frequency and gmts of lta-positive sera varied with the origin of the patient (chinese greater than north african greater than caucasian, thus paralleling the risk for npc in each ethnic group) and the stage of the disease (stage iv greater than stage i). a positive correlation was found between lta and anti-ebv titres with regard to anti-vca and anti-ebna antibodies. sma were spe ...1978215532
in vivo cell-mediated immunity in chinese patients with nasopharyngeal carcinoma.delayed hypersensitivity to antigens derived from four ebv-related (khly 28, raji, f265 and nc37) and one ebv-unrelated (molt) t-lymphoid cell lines and to standard antigens (trichophyton, candida albicans and streptokinase/streptodornase) was measured in 104 npc patients and 24 patients with other cancers. of the npc patients with non-disseminated disease, 55/86 (63.2%) had a positive skin reaction to hkly 28 extract, compared with only 1/18 (5.6%) oc patients with non-disseminated disease. the ...1978215533
use of immunological studies in evaluating the clinical course of nasopharyngeal carcinoma.the prognosis of a npc patient is influenced by many factors, including the therapeutic method used and the clinical stage and histological characteristics of the tumour. the immune response of the peripheral lymphocytes was examined in 27 patients with npc, and it was concluded that this response accurately indicates the clinical course of the diseases as compared with clinical stage of disease, histological type of tumour and anti-vca-antibody titre.1978215534
nasopharyngeal carcinoma: etiology and control. recommendations. 1978215535
animal models for nasopharyngeal carcinoma. 1978215536
burkitt's lymphoma in iraq. clinical and pathological study of forty-seven patients.the clinical and pathological features of 47 patients with burkitt's lymphoma seen at the university of baghdad teaching hospital over the years 1969-1977 are described. there were 30 males and 17 females with a mean age of 7.5 years. all patients presented with advanced disease; 44 were in stage iii and 3 in stage iv, (1 with central nervous system involvement; 1 with bone-marrow involvement; 1 presented as acute leukaemia). the abdomen, with or without other sites, was involved in 42 patients. ...1979215559
14q+ marker chromosome in an ebv-genome-negative lymph node without signs of malignancy in a patient with ebv-genome-positive nasopharyngeal a patient with an ebv-genome-positive nasopharyngeal carcinoma, an ebv-genome-negative inguinal lymph node without histological evidence of malignant lymphoma or metastatic carcinoma growth was found to contain a 14q+ marker chromosome, identified as an 8;14 translocation, in all cells analyzed. this observation indicates that chromosome aberrations may precede histological signs of malignancy. the possible implication of this finding in relation to the postulated role of the 14q+ marker and ...1979215561
correlated expression of a b-lymphocyte-specific glycoprotein (gp27/35) and the ebv receptor/c3 receptor complex in sublines from the same burkitt lymphoma.a series of virus-producer and non-producer sublines, derived from the burkitt lymphoma line jijoye and its p3hr-1 clone, were previously found to differ in the expression of ebv receptors and, in parallel, c3 receptors. the differences could be related to an "internal selection" caused by the cytopathic p3hr-1 virus variant, favouring the growth of receptor-negative cells. we have now analyzed the same lines for the expression of a b-lymphocyte-specific glycoprotein (gp27/35). a close parallell ...1979215562
cell-density-dependence for growth in agarose of two human lymphoma lines and its decrease after epstein-barr virus conversion.we have compared the growth in agarose medium of two ebv-negative burkitt lymphoma lines, bjab and ramos, and that of their ebv-converted derivatives. optimal conditions for growth in agarose medium are described. the original ebv-negative lines can grow in agarose to a high efficiency, provided a critical level of cell concentration is attained. below this level, there is no growth at all. ebv-converted sublines form colonies at a much lower cell concentration, and their maximal plating efficie ...1979215564
biochemical identification of primate lymphoid cell-surface glycoproteins.we have employed the galactose oxidase-tritiated sodium borohydride labelling method to examine the surface glycoproteins of cotton-topped marmoset and other primate cell lines either established from tumors or transformed in vitro by different lymphotropic herpesviruses. the labelled surface glycoproteins were separated on acrylamide gels in the presence of sodium dodecyl sulfate (sds) and analyzed by fluorography. our results indicate that (1) lymphocytes of the same class from different prima ...1979215565
[early granuloma formation, a rise in eb virus antibody titer with an interesting ultrastructural observation and reticulum cell sarcoma-like pattern--an autopsy case]. 1978215801
epstein-barr virus (ebv) antibody in patients treated by radical radiotherapy for head and neck cancer.antibody against epstein barr viral capsid (ebv-vca) was measured in 65 patients with primary head and neck cancer referred for radical radiotherapy. sixty-three percent had locally advanced cancer and 37% had early disease. forty-eight percent had detectable antibody in their sera. thirty-three percent had elevated titers (above 1:20 dilution). only 19% of cases with early cancer had increased titers. almost 50% of those with locally advanced cancer or recurrence following treatment had titers ...1978215844
[recent data on the pathogenic role of the epstein-barr virus]. 1978215968
[epstein-barr virus and infectious mononucleosis. a clinical study of epstein-barr antibodies (author's transl)].thirty-seven patients--aged 2 to 58--with clinical and hematological signs of infectious mononucleosis were studied. thirty patients gave serologic evidence of epstein-barr virus infections; antibodies to viral capsid antigen (vca) appeared very early (only two seroconversions and 4 cases of increased titer were detected); antibodies to early antigen (ea) occured in 25 patients, but only 9 had high titers; antibodies to nuclear antigen (ebna) appeared late in the course of the disease. vca-speci ...1978215969
infectious mononucleosis and epstein-barr virus. 1. epidemiology, pathogenesis, immune response.the evidence linking epstein-barr virus causally with infectious mononucleosis is compelling. the disease occurs only in persons who lack antibody to this virus, and serologic findings during the acute illness provide almost certain evidence for its etiologic association. additional evidence is derived from transformation studies using lymphocytes from persons infected with epstein-barr virus. epidemiologic studies have related the need for close contact with salivary secretions to the low commu ...1979215976
infectious mononucleosis and epstein-barr virus. 2. clinical picture, diagnosis, management.the diagnosis of infectious mononucleosis is confirmed by hematologic, biochemical, and serologic data. an absolute increase in peripheral mononuclear cells to at least 4,500/cu mm is an essential feature. the serologic hallmark of the disease is the presence of heterophile antibody. the age of the patient may affect the clinical presentation and laboratory findings. therapy is largely supportive, with steroids being used only in the presence of certain severe complications.1979215977
biology of the human malignant lymphomas. 1978216061
detection of antibody to a new antigen induced by epstein-barr virus in rheumatoid arthritis.a new antigen which is different from epstein-barr virion antigen was detected in nc-37 cells infected with epstein-barr virus (ebv). a significant elevation of the titer of antibody to this new antigen was observed in the sera of the patients with rheumatoid arthritis (ra), but not in the sera of controls. from the standpoint of the etiologic role of ebv in ra, it is interesting that the antibody to the new antigen is not detected in the healthy persons in contrast to other viral antibodies. th ...1979216133
herpesviruses, venereal disease, and cancer. 1978216293
establishment of a malignant, epstein-barr-virus (ebv)-negative cell-line from the pleura effusion of a patient with hodgkin's disease. 1979216443
the interaction of hydrocortisone, interferon and the epstein--barr virus in lymphoid cells. 1978216555
enhanced induction of growth of b lymphoblasts from fresh human blood by primate type-c retroviruses (gibbon ape leukemia virus and simian sarcoma virus). 1979216638
a comparison of the prognostic value of antibody-dependent lymphocyte cytotoxicity and other ebv antibody assays in chinese patients with nasopharyngeal carcinoma.antibody titres to ebv-associated antigens in chinese npc patients were analysed according to length of survival after diagnosis and to disease stage. geometric mean titres of adlc antibody were highest in the long-term survivors, whereas vca, ea and ebna antibody titres showed an inverse relationship to survival. high vca, ea and ebna titres were less frequent, and high adlc titres were more frequent in long-term survivors than in intermediate or short-term survivors. the association of geometr ...1979216639
antibody responses to herpesvirus papio antigens in baboons with epstein-barr virus-related herpesvirus, termed herpesvirus papio (hvp), was isolated from baboons (papio hamadryas) at the institute of experimental pathology and therapy, sukhumi, ussr, where there is a continuing outbreak of lymphoma. in the present study sera from diseased baboons and from age- and sex-matched control animals were examined for antibodies to hvp antigens. results showed that animals with lymphoid disease had antibodies to hvp virus capsid, early, soluble, and nuclear antige ...1979216640
differences in ebv receptor concentration between in vitro ebv-converted lymphoma sublines reflect biological differences between the converting viral substrains.we have compared the ebv-receptor concentration of two originally ebv-negative human b-cell lymphoma lines, after in vitro conversion with the transforming b95-8 or the cytopathic p3hr-1 eb-viral substrain, respectively, into permanent ebv-carrying sublines. receptors were measured by the quantitative ebv-absorption bioassay of sairenji and hinuma (1973). ebv receptor concentration of all p3hr-1 virus-converted sublines was significantly reduced, in comparison with the b95-8 virus-converted subl ...1979216641
characterization of thymus-derived lymphocyte subsets in acute epstein-barr virus-induced infectious mononucleosis.changes in thymus-derived (t) lymphocyte subpopulation numbers were studied in patients with acute and convalescent epstein-barr virus (ebv)-induced infectious mononucleosis (lm). t cell subsets were characterized by the presence of fc receptors for igg (tg), for igm (tm) or by the absence of either receptor (tnon-m, non-g). we found that in acute im, total numbers of t and b lymphocytes were elevated (p less than 0.01). of the t lymphocyte subsets, the total number of tnon-m, non-g lymphocytes ...1979216749
epstein-barr virus antibodies in sudanese patients with nasopharyngeal carcinoma: a preliminary report.with the use of an immunofluorescence technique epstein-barr viral capsid antigen antibody titers were determined in the sera from 226 sudanese: 41 with nasopharyngeal carcinoma (npc), 77 with other head and neck cancers, 21 with malignant lymphomas, 63 with other cancers, 6 with specific granulomas, and 18 normal controls. of the npc patients, 87.8% had titers of 320 or greater and 43.9% had titers of 2,560 or more, whereas none had titers of less than 40. their geometric mean titer (gmt) level ...1979216831
translocation of a hydrocarbon fluorescent probe between epstein-barr virus and lymphoid cells: an assay for early events in viral infection.translocation of the hydrocarbon fluorescent probe diphenylhexatriene (dph) between membranes was studied by fluorescence polarization (p) analysis. first, using a model system, the high p value (0.324) of dph-labeled cholesterol/phosphatidylcholine liposomes and the low p value (0.157) of dph-labeled phosphatidylcholine liposomes allowed detection of dph translocation between interacting liposomes. this was monitored by the change in p in either direction. early events during cell-virus interac ...1978217012
epstein-barr virus and human chromosomes: close association of the resident viral genome and the expression of the virus-determined nuclear antigen (ebna) with the presence of chromosome 14 in human-mouse hybrid cells.fourteen hybrid clones derived from the fused cultures of human lymphoblastoid fv5 cells and 5-bromodeoxyuridine-resistant mouse fibroblastic mcb2 cells grown in hypoxanthine/aminopterin/thymidine selective medium were examined for the presence of epstein-barr virus (ebv) dna, the expression of the virus-determined nuclear antigen (ebna), and the presence of human chromosomes, in the course of serial passage in vitro. among the hybrid clones tested, 3 were positive for ebv dna and ebna, whereas ...1978217014
differential effect of phosphonoacetic acid on early antigen synthesis in two epstein-barr virus producer cell lines. 1979217163
infectious mononucleosis and related syndromes.the "mono syndrome" is an acute febrile disease of older children and young adults which involves the lymphatic system and is characterized hematologically by the presence of 50% or more lymphocytes and monocytes and 10% or more atypical lymphocytes. epstein-barr virus (ebv) causes over 90% of the syndrome, cytomegalovirus (cmv) about 5% to 7%, and toxoplasma gondii less than 1%. viral hepatitis, adenovirus, rubella, and herpes simplex are rare causes. only ebv produces classical heterophile-pos ...1978217270
infection with epstein-barr virus. 1979217475
genetic mechanisms in cancer predisposition: report of a cancer family.a family is described in which four children developed cancer affecting different organs:lymphoma, meningeal sarcoma, osteogenic sarcoma, and adenocarcinoma of the cecum. since there was only one other case of cancer in previous generations of this family, an hypothesis is put forth to explain this unusual aggregation on the basis of recombination of common genes. it is postulated that each parent carried a different combination of genes which, though not associated with increased cancer predisp ...1979217526
characterization of cross-reacting antigens on the epstein-barr virus envelope and plasma membranes of producer cells.a rabbit antiserum has been prepared against the b95-8 transforming strain of ebv. the antiserum has a high virus neutralizing titer (approximately 1:1000) against both the marmoset b95-8 ebv and the human p3hr-1 ebv. the neutralizing antibodies may be absorbed completely with ebv producer cell lines, but not with nonproducer cell lines or producer cell lines treated with phosphonoacetic acid (paa) so as to be nonproducer. after repeated absorption with paa-treated b95-8, the serum remains react ...1979217544
discordance of epstein-barr virus (ebv) specific humoral and cellular immunity in patients with malignant lymphomas: elevated antibody titres and lowered in vitro lymphocyte reactivity. 1978217561
susceptibility of lymphoblastoid cells to infection with human cytomegalovirus.human lymphoblastoid cells of b- and t-cell origin were examined for their in vitro susceptibility to infection with human cytomegalovirus (cmv). results of infectious-center assays, at virus to cell ratios of 10, indicated that in each of the lymphoblastoid cell lines tested less than 1% of the cells produced infectious virus. under these conditions, cmv specific antigens were undetectable. infection of lymphoblastoid cells with cmv resulted in atypical virus growth curves similar to those obta ...1979217829
[clinical significance of sensitive and specific variations of the paul-bunnell reaction]. 1979218156
tumor initiators and promoters in the induction of epstein-barr virus.the effect of various tumor initiators and promoters on induction of persisting epstein-barr virus (ebv) in different lines of lymphoblastoid cells was analyzed. neither five polycyclic aromatic hydrocarbons, amongst them potent tumor initiators (e.g., 7,12-dimethylbenz[a]anthracene), nor the potent (ultimate) liver carcinogen n-acetoxy-n-2-acetylamino-fluorene induced ebv. a series of compounds, representing three classes of tumor-promoting diterpene esters (e.g., 12-o-tetradecanoylphorbol-13-a ...1979218219
cytomegalovirus granulomatous hepatitis.three patients with granulomatous hepatitis due to cytomegalovirus are described. they are compared to the three previously described patients with this disease, and their clinical and serologic characteristics are discussed. similarities and differences between infectious mononucleosis (epstein-barr virus) and cytomegalovirus infections are adduced. that cytomegalovirus may be a cause of granulomatous hepatitis in the adult is stressed.1979218449
simultaneous infection with multiple herpesviruses. 1979218450
effects of nucleoside analogs on epstein-barr virus-induced transformation of human umbilical cord leukocytes and epstein-barr virus expression in transformed cells.two methods of assay, measuring (i) stimulation of host cell dna synthesis by [(3)h]thymidine incorporation and (ii) morphological transformation in microtiter plates, were employed to determine what effect treatment during infection with adenine arabinoside or 5-iodo-5'-amino-2',5'-dideoxyuridine has on epstein-barr virus (ebv) (b95-8)-induced transformation of human umbilical cord leukocytes. it was found that adenine arabinoside inhibited ebv-induced transformation in a dose-dependent manner ...1979218497
hla-d locus typing in ankylosing spondylitis and reiter's syndrome.hla-d typing of 44 patients with ankylosing spondylitis (as) and 31 patients with reiter's syndrome (rs) did not show increased frequency of any particular dw allele in either population of patients as compared to controls. such studies also allowed each patient's general response to be compared with other general responses within each experiment. contrary to reports of diminished lymphocyte responses in as patients, hyperresponsiveness in both as and rs patients was found.1979218595
request for specimens from organ transplant and immunodeficiency patients who developed lymphomas. 1979218723
analysis of the transformation of human lymphocytes by epstein-barr virus. i. sequential occurrence from the virus-determined nuclear antigen synthesis, to blastogenesis, to dna synthesis.a b-cell population of human cord blood lymphocytes was exposed to the b95-8 strain of epstein-barr virus (ebv), and simultaneous observations of immunofluorescence, cellular morphology and autoradiography were carried out in each individual cell. it was evident that ebv-determined nuclear antigen (ebna) synthesis occurred prior to blastogenic response and dna synthesis. ebna-positive cells could be observed as early as 12 h after infection and reached a maximum of 17% at 24 h, followed by a pla ...1979218892
replication of herpes simplex virus in human b lymphocytes stimulated by epstein-barr virus.human b lymphocytes prepared from adult blood or cord blood by the use of neuraminidase-treated sheep red blood cells which did not respond mitogenically to phytohemagglutinin or pokeweed mitogen, responded well to the b95-8 strain of epstein-barr virus, but not to the p3hr-1 strain, with increased incorporation of tritiated thymidine. replication of herpes simplex virus could be demonstrated in cultures of b lymphocytes preincubated with the b95-8 strain.1979218893
sensitive solid-phase radioimmunoassay for detection of human immunoglobulin g antibodies to varicella-zoster virus.a sensitive solid-phase radioimmunoassay for detection of antibodies to varicella-zoster virus (vzv) is described. the antigen consisted of a sonically disrupted extract of vzv-infected human embryo cells. 125i-labeled rabbit anti-human immunoglobulin g (igg) specific for the fc portion of human igg was used to detect human igg bound to viral antigen. with this technique, 193 human sera were evaluated for their igg antibody titer against zvz. subjects included 62 healthy adults, 33 young childre ...1979219015
characterization of an epstein-barr virus-induced dna polymerase.the addition of iododeoxyuridine to p3hr-i cell cultures led to a large increase in both epstein-barr virus (ebv)-induced dna polymerase activity and early antigen-positive cells. this ebv-induced dna polymerase was separated from the cellular alpha- and beta-polymerases by sequential column chromatography on sepharose 6b, deae-cellulose, and phosphocellulose, resulting in partial purification of about 320-fold. the partially purified-ebv dna polymerase could be distinguished from the cellular d ...1979219209
epstein-barr virus-specific rna. iii. mapping of dna encoding viral rna in restringent infection.namalwa and raji cells, originally obtained from a burkitt tumor biopsy, grow as continuous cell lines in vitro and contain the epstein-barr virus (ebv)-related nuclear antigen ebna (b. m. reedman and g. klein, int. j. cancer 11:499-520, 1973) and rna homologous to at least 17 and 30% of the ebv genome, respectively (s. d. hayward and e. kieff, j. virol. 18:518-525, 1976; t. orellana and e. kieff, j. virol. 22:321-330, 1977). the polyribosomal and polyadenylated [poly(a)+] rna fractions of namal ...1979219221
nucleosomal structure of epstein-barr virus dna in transformed cell lines.micrococcal nuclease digestion was used to analyze epstein-barr virus (ebv) dna structure in nuclei of transformed cells. digests of virus-producing (p3hr-1), non-virus-producing (raji), and superinfected rajii cell nuclei were fractionated by electrophoresis on agarose gels, transferred to nitrocellulose, and hybridized to 32p-labeled ebv dna. the viral dna of raji nuclei produced a series of bands on electrophoresis whose lengths were integral multiples of a unit size, which was the same as th ...1979219253
size of the intracellular circular epstein-barr virus dna molecules in infectious mononucleosis-derived human lymphoid cell lines.the size of non-integrated circular epstein-barr virus (ebv) dna molecules isolated from seven different human lymphoblastoid cell lines of infectious mononucleosis origin has been determined by sedimentation analysis and by direct contour length measurements on electron micrographs. six lines had intracellular circular ebv genomes of the same size as linear virion dna molecules. the seventh line, established with the b95-8 strain of ebv, was the only one found to have circular ebv dna molecules ...1979219261
retinoic acid inhibition of epstein-barr virus induction. 1979219367
[blood transfusion and viral diseases. recent acquisitions concerning viral hepatitis viruses, cytomegaloviruses and epstein-barr virus].in recent years, an increasingly clear picture has been formed of the virus-induced syndromes that may follow a blood transfusion or the use of blood derivatives. up to about 10 years ago, post-infusion infection was predominantly due to serum hepatitis. blumberg's discovery of hbsag (formerly known as australia antigen) has made it possible to check and prevent viral hepatitis, type b, and to recognise such distinct forms as the mononucleosis-like syndrome caused by cytomegalic virus, infectiou ...1979219399
epstein-barr virus antibody responses and clinical illness in renal transplant recipients.among 88 renal transplant recipients evaluated for a change in epstein-barr virus (ebv) antibody status in the period after transplant, 22 showed a 4-fold rise and eight showed an 8-fold or greater rise in ebv antibody. among the patients with an 8-fold or greater ebv antibody rise, the occurrence of fever was frequent, one patient developed a lymphoproliferative reaction, and one died with a malignant ebv infection. patients without pretransplant antibody showed a longer mean time to antibody r ...1979219556
viral serology (hepatitis b virus, cytomegalovirus, epstein-barr virus) and abnormal liver function tests in transfused patients with hereditary hemorrhagic diseases. 1979219564
identification of the soluble hvp-associated antigen of the lymphoblastoid cell line established from lymphomatous baboon (papio hamadryas).a new technique (indirect double immunodiffusion) for detection of ebv-associated soluble antigen and corresponding antibodies has been developed. this technique includes three steps: 1) simple double immunodiffusion with extracts of raji cells (or other ebv-genome positive cells) and human sera containing antibodies against ebv-associated soluble antigen; 2) extensive washing and treatment with anti-human globulin; 3) extensive washing and treatment with tannic acid. using this test it was show ...1978219644
anti-gm and anti-inv antibodies in children during the course of clinical infectious mononucleosis syndrome.forty-eight children with the symptoms of infectious mononucleosis were examined. in six of then anti-gm(1) antibodies were discovered, in two anti-gm (21), in one child anti-inv (1).1978219804
identification of human lymphocyte subpopulations by surface marker analysis. 1979219922
[cytomegalovirus and eb-virus antibodies in patients with non-a non-b hepatitis (author's transl)]. 1979219957
epstein-barr virus-related antibody patterns in ataxia-telangiectasia.epstein-barr virus-related antibody titres were determined in twenty-seven patients with ataxia-telangiectasia (at) and twenty-two healthy members of their families, in twenty-two patients with other diseases, among them ten with behçet's disease and ten with various primary immune deficiencies, and fifteen healthy members of their families, and in twenty-three unrelated healthy controls. the at patients showed an increased incidence (55.6%) of high antibody titres (greater than or equal to 1:32 ...1979219976
[125i] clq-binding activity and its relationship with anti-epstein--barr virus antibodies in sera from nasopharyngeal carcinoma patients. 1979220051
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