[on antigen relationship between herpes simplex virus, herpes virus platyrrhinae (marmoset herpesvirus) and equine abortion virus]. 19694318305
deoxyribonucleic acid synthesis in synchronized mammalian kb cells infected with herpes simplex virus.we examined the patterns of host cell and virus deoxyribonucleic acid (dna) synthesis in synchronized cultures of kb cells infected at different stages of the cell cycle with herpes simplex virus (hsv). we found that the initiation of hsv dna synthesis, we well as the production of new infectious virus, is independent of the s, g1, and g2 phases of the mitotic cycle of the host cell. this is in contrast to data previously found with equine abortion virus. because hsv replicates independently of ...19714327586
paralysis of horses associated with equine herpesvirus 1 infection. 19714328999
[virological studies of the central nervous system of horse fetuses and findings in mares suffering from central nervous system disease following abortion caused by equine herpesvirus 1]. 19724334255
ehv-1: a recurrent problem. 19892543119
equine herpesvirus 1: biological and biophysical comparison of two viruses from different clinical entities. 19744376548
morphological components of herpesvirus. i. intercapsomeric fibrils and the geometry of the capsid.highly purified equine herpesvirus type 1 (ehv-1) and herpes simplex virus type 1 (hsv-1) were observed by electron microscopy in various stages of disintegration. under the conditions used, envelopes of virions usually were fragmented, and virus capsids collapsed in situ into flattened sheets of capsomeres. a matrix of intercapsomeric fibrils was observed. evidence suggesting that the capsid hexamer has threefold structural symmetry is presented.19744376809
effect of antiviral agents in equine abortion virus-infected hamsters.equine abortion virus, a member of the herpesvirus group, produces a lethal infection in hamsters. with this system, the protective effect of certain inhibitors of deoxyribonucleic acid viruses, inducers of interferon and exogenous interferon, was evaluated. of the various agents studied, 9-beta-d-arabinofuranosyladenine markedly suppressed mortality, and 5-iodo-2'-deoxyuridine, distamycin a, and n-ethylisatin beta-thiosemicarbazone were inactive. of the inducers tested, statolon, ultraviolet-ir ...19724376907
[effect of air humidity on the tenacity of equine herpesvirus 1 (rhinopneumonitis virus) using different carriers]. 19734692643
equine herpesviruses: antigenic relationships and deoxyribonucleic acid densities.equine herpesviruses with a deoxyribonucleic acid density of 1.716 to 1.717 g/cm(3) were compared with one another by the plaque-reduction test and by the rate of development of cytopathic effect as indicated by plaque size in rabbit kidney cultures. of the 19 isolates studied, the 9 which had already been tentatively labeled equine abortion viruses were serologically similar to one another; each of them grew more quickly than did any of the other 10 isolates although the mean plaque sizes forme ...19734742974
equine herpesvirus outbreaks. 19892543120
comparison of four horse herpesviruses.four equine herpesviruses (equine abortion virus, equine herpesvirus types 2 and 3, and equine cytomegalovirus) were compared. the equine abortion virus did not cross-neutralize with any of the other viruses, but the other three did show varying degrees of cross-neutralization among themselves. equine abortion virus grew more quickly in tissue cultures than did the others, and attained higher titers of infectivity in the culture fluid; it also formed plaques in a wider range of tissue culture sp ...19694982831
equine herpesvirus type 1: the l- character in two of forty-three field isolates. brief report. 19725063121
nucleic acid and protein synthesis in kb cells infected with equine abortion virus (equine herpesvirus type 1). 19715099956
[research into the inactivation effect of surface-active substances on the rhinopneumonitis virus in horses (equine abortion virus)]. 19685683868
mycoplasmal deoxyribonuclease activity in virus-infected l-cell cultures.cell-free extracts of mycoplasma hominis and medium from 72-hr broth cultures had deoxyribonuclease activity like that of deoxyribonuclease i. mg(++) stimulated activity, and the ph optimum was between 8.0 and 9.0. double-stranded or heatdenatured deoxyribonucleic acid (dna) served as a substrate, and oligonucleotides were produced. cell-free extracts of l cells infected with m. hominis or m. hominis plus equine abortion virus (equine herpes virus, eav) had greatly increased activity over that o ...19695782042
[electron optical demonstration of the equine abortion virus with the aid of the negative contrast procedure]. 19645890648
[characterization of an equine abortion virus from poland and comparison with known equine rhinopneumonitis virus strains]. 19655894190
biological and morphological aspects of the growth of equine abortion virus.darlington, r. w. (st. jude children's research hospital, memphis, tenn.), and c. james. biological and morphological aspects of the growth of equine abortion virus. j. bacteriol. 92:250-257. 1966.-the growth of equine abortion virus (eav) was studied by bioassay and electron microscopy in l-cell monolayer and suspension cultures, and in hela and bhk 21/13 cell monolayers. results of virus assay (plaque-forming units) indicated that production of cell-associated virus (cav) began at 6 to 9 hr af ...19665941279
the antiviral spectrum of (e)-5-(2-bromovinyl)-2'-deoxyuridine.the antiviral activity spectrum of (e)-5-(2-bromovinyl)-2'-deoxyuridine (bvdu) is not restricted to herpes simplex virus type 1 (hsv-1) and varicella-zoster virus (vzv) but also encompasses several other herpesviruses such as suid herpesvirus type 1 (shv-1), bovid herpesvirus type 1 (bhv-1), simian varicella virus (svv), herpesvirus saimiri, herpesvirus platyrrhinae, and the baculovirus trichoplusia ni multiple nuclear polyhedrosis virus. other herpesviruses such as herpes simplex virus type 2, ...19846092320
vaccination against equine herpesvirus 1 and equine influenza infection. 19846093330
identification and nucleotide sequence of a gene in equine herpesvirus 1 analogous to the herpes simplex virus gene encoding the major envelope glycoprotein gb.a gene in equine herpesvirus 1 (ehv-1; equine abortion virus) equivalent to the gb glycoprotein gene of herpes simplex virus (hsv) has been identified by dna hybridization and nucleotide sequencing. a 4.3 kbp ehv-1 psti-clai sequence (0.40 to 0.43 map units) contained an open reading frame flanked by appropriate control elements and was capable of encoding a polypeptide of 980 amino acids. this had 50 to 60% identity over a 617 amino acid conserved region with the gb gene products of hsv and thr ...19892543744
identification of the gb homologues of equine herpesvirus types 1 and 4 as disulphide-linked heterodimers and their characterization using monoclonal antibodies.equine herpesvirus types 1 and 4 (ehv-1 and ehv-4) labelled with [14c]glucosamine were purified from infected cell culture medium and profiles of their structural proteins were obtained that enabled identification of the major glycoproteins. nine glycosylated polypeptides were identified for each virus. preparations of the purified viruses each contained a glycoprotein which was linked by disulphide bonds, as determined by diagonal gel electrophoresis under reducing/non-reducing conditions. high ...19892543773
characterization of two equine herpesvirus (ehv) isolates associated with neurological disorders in horses. 19872829471
pathogenicity of equine herpesvirus 1 subtype 2 for foals and adult pony mares.three pony mares and 4 pony foals were inoculated with a subtype 2 strain of equine herpesvirus 1. foals had periods of fever 12 h and 2.5 days after inoculation and leukopenia, involving both neutrophils and lymphocytes, followed by leukocytosis. mares had transient fever and leukopenia 24 hours after inoculation that were less severe than in foals. an increase in circulating virus-neutralizing antibody was seen in 2 of 3 inoculated mares, but not in foals. attempts to isolate virus from blood ...19846095515
[neurological disorders caused by equine herpesvirus type 1 and cauda equina neuritis in horses].the differences in aetiology, symptomatology, pathomorphology, diagnosis and therapy between the nervous form (paralytic form) of equine herpes virus, type 1, and neuritis caudae equinae are reviewed. the conclusion is that in most cases it is possible to differentiate between these two clinical syndromes.19846096984
specificity of pseudorabies virus serotests.pigs experimentally inoculated with bovine herpesvirus-1 or equine herpesvirus-1 developed mild clinical disease signs. regression of clinical disease was accompanied by development of specific virus-neutralizing antibodies. these antibodies did not react positively with pseudorabies antigens in the serum-virus neutralization test, an indirect radioimmunoassay, or a microimmunodiffusion test.19846098206
genetic relatedness and colinearity of genomes of equine herpesvirus types 1 and 3.the arrangement and location of homologous dna sequences within the genomes of equine herpesvirus type 1 (ehv-1) and ehv-3 were investigated by using southern blot hybridization analyses conducted under stringent conditions. recombinant plasmid libraries comprising 95 and 84% of the ehv-1 and ehv-3 genomes, respectively, were labeled with 32p-deoxynucleotides by nick translation and were used as probes in filter hybridization studies. the dna homology between the ehv-1 and ehv-3 genomes was disp ...19863005628
molecular epidemiology and pathogenesis of some equine herpesvirus type 1 (equine abortion virus) and type 4 (equine rhinopneumonitis virus) isolates.representative strains of ehv isolated from an aborted foetus and from a horse with rhinopneumonitis in new zealand had restriction endonuclease dna fingerprints typical of those usually associated with these syndromes elsewhere and now designated ehv1 and 4 respectively. ehv1 was isolated from the brain and spinal cord of a 4-year-old gelding that died of myeloencephalitis. a mare on the same farm, at about the same time as the gelding developed myeloencephalitis, aborted and ehv1 was isolated ...19846099117
studies on cross-reactive antigens in the herpesviruses. 19816162270
the prospects for antiviral chemotherapy in veterinary medicine. 19816167058
antigenic and structural conservation of herpesvirus dna-binding proteins.previously, we have shown a common antigen of several herpesviruses (pseudorabies virus, equine abortion virus and bovine mammillitis virus) to be antigenically related to the major dna-binding proteins of herpes simplex virus types 1 and 2. in this study we have purified the cross-reacting polypeptide from cells infected with pseudorabies virus, equine abortion virus and bovine mammillitis virus and shown the cross-reacting protein to be a major dna-binding protein for each virus. tryptic pepti ...19816171613
chronic production of defective-interfering particles by hamster embryo cultures of herpesvirus persistently infected and oncogenically transformed cells.highly passaged defective-interfering (di) particle preparations of equine herpesvirus type 1 (ehv-1) were found to mediate the co-establishment of persistent infection and oncogenic transformation of permissive hamster embryo cells. four cell lines, designated di-1 to di-4, were shown to possess biological properties typical of transformed cells and to induce the rapid formation of metastatic fibrosarcomas when injected into syngeneic lsh hamsters. corresponding di tumour cell lines, designated ...19826178795
propagation and quantitation of animal herpesviruses in eight cell culture systems.a comparative study was carried out to determine the relative sensitivities of eight different cell culture systems to six different herpesviruses of animals. the cells used were: ofl (ovine fetal lung), ml (mink lung), fk (ferret kidney), ptk-2 (potoroo kidney), tek (turkey embryo kidney), ed (equine dermal), bt (bovine turbinate), and pk15 (porcine kidney). the viruses tested were: prv (pseudorabies) of swine, cphv (caprine herpesvirus), ibrv (infectious bovine rhinotracheitis virus), dn-599 s ...19882846231
immunologic relationships between equine herpesvirus type 1 (equine abortion virus) and type 4 (equine rhinopneumonitis virus).the specificity of selected immune responses to equine herpesvirus type 1 (ehv-1) and type 4 (ehv-4) was examined in 3 colostrum-deprived specific-pathogen-free foals. single foals were vaccinated with inactivated ehv-1, inactivated ehv-4, or control cell lysate plus adjuvant followed by successive intranasal challenge exposures with ehv-1 and ehv-4 or with ehv-4 and ehv-1. vaccination with inactivated virus preparations elicited cellular immune responses and antibody which were augmented by sub ...19846208822
oncogenic transformation by equine herpesviruses (ehv). i. properties of hamster embryo cells transformed by ultraviolet-irradiated ehv-1. 19806244694
specific inhibition of herpesvirus ribonucleotide reductase by synthetic peptides.ribonucleotide reductase is an essential enzyme for dna synthesis in all prokaryotic and eukaryotic cells; it catalyses the reductive conversion of ribonucleotides to deoxyribonucleotides. several herpesviruses including herpes simplex virus type 1 (hsv-1), hsv-2, pseudorabies virus (prv), equine herpesvirus type 1 (ehv-1) and epstein-barr virus (ebv) have been found to induce novel ribonucleotide reductase activities. there is evidence that the hsv-1 ribonucleotide reductase activity is virus-e ...19863012359
ehv1 and equine paresis. 19806246670
differentiation of equine herpesviruses of type 1. 19806250526
differentiation of subtypes within equine herpesvirus type 1. 19806250527
clinical report of a paralytic syndrome affecting stallions, mares and foals on a thoroughbred outbreak of ataxia and paralysis on a thoroughbred studfarm is reported. the cause of the disease was attributed to equid herpesvirus (ehv1) infection which stemmed from a single 10-month abortion on the studfarm. stallions, mares and foals were all affected but the most serious clinical signs occurred in the mares. there were 35 out of 39 mares, 2 out of 4 stallions and 5 out of 39 foals which exhibited signs of ataxia. nine mares became recumbent and died or were euthanased. treatment with ...19806250820
pathological observations on an outbreak of paralysis in broodmares.a description is given of the pathological changes present in 8 mares which died or were euthanased in the course of an outbreak of paralysis on a thoroughbred studfarm. in all cases the principal changes were in the central nervous system (cns) and consisted of a severe and widespread vaculitis in the brain, cord, sheaths of nerves, capsules of ganglia and occasionally elsewhere in the body. associated with the damage to vessel walls there was haemorrhage and exudation of plasma into the periva ...19806250821
vaccination of pregnant ponies against equine rhinopneumonitis.bovine herpesvirus 1247 (one dose) was given subcutaneously to five pregnant pony mares between 227 and 319 days of their gestations. there were no adverse clinical reactions, and the virus was not recovered from nasal swabs collected during a 2-week period after vaccination. four ponies foaled full-term, live, healthy foals. the foal of the fifth mare (no. 1) was found dead, but on the basis of the pathologic and virologic examinations, the virus was not considered to be the cause of the death. ...19806254412
oncogenic transformation by by equine herpesviruses. ii. coestablishment of persistent infection and oncogenic transformation of hamster embryo cells by equine herpesvirus type 1 preparations enriched for defective interfering particles.infection of permissive hamster embryo cells with virus preparations enriched for defective interfering (di) particles of equine herpesvirus type 1 (ehv-1) resulted in persistent infection and oncogenic transformation. six cell lines, designated di-5 to -10, exhibited biological properties (immortality, increased saturation density, growth in soft agar, etc.) inherent to transformed cells, but 2 to 18% of the total cells in these cell lines were shown to release virus as judged by electron micro ...19806255207
response of pregnant mares to equine herpesvirus 1 (ehv1).twenty-one pregnant mares were inoculated with ehv1. nineteen became infected as evidenced by clinical signs and/or viremia but only one mare aborted a virus-infected fetus. the viremias were leukocyte-associated and appeared to be non-productive, latent infections of these cells. infectivity, detectable by cocultivation, persisted in the circulating leukocytes for as long as 9 days without resulting in abortion. the data suggest that it is extremely difficult to evaluate the efficacy of vaccine ...19806257449
a common code of practice for the control of contagious equine metritis and other equine reproductive diseases for the 1981 covering season in france, ireland and the united kingdom. 19806258284
serologic responses of pregnant thoroughbred mares to vaccination with an inactivated equine herpesvirus 1 vaccine.the immunogenic potency and safety of a chemically inactivated equine herpesvirus 1 vaccine with added adjuvant was evaluated by testing serum-neutralizing and complement-fixation antibody responses of pregnant thoroughbred mares. the vaccinated population comprised 321 pregnant mares on 7 farms; 3 in normandy, france; 1 in kildare, ireland; and 3 in central kentucky. the pattern of antibody response to vaccination was found qualitatively and quantitatively similar to that of pregnant mares prev ...19806259970
lymphocyte responses to virus and mitogen in ponies during experimental infection with equine herpesvirus 1.six pony foals, experimentally infected with equine herpesvirus 1 (ehv-1), were studied for their lymphocyte responses to ehv-1 and phytohemagglutinin (pha) stimulations. lymphocyte blastic transformation in the presence of ehv-1 appeared as early as 2 days after the foals were inoculated, reached a peak in 7 to 10 days, and subsequently decreased. in contrast, the lymphocyte blastic transformation in the presence of pha increased sharply, reaching a peak in 2 to 3 days, and then decreased to it ...19806259978
complement requirement for virus neutralization by antibody and reduced serum complement levels associated with experimental equine herpesvirus 1 infection.pony foals, negative for detectable serum-neutralizing antibody to equine herpesvirus 1 by the standard tube-culture virus neutralization test, were experimentally infected with equine herpesvirus 1. complement-requiring (cr) and non-complement-requiring (ncr) serum-neutralizing antibodies were evaluated in preinfection and postinfection sera by means of a complement-enhanced plaque reduction assay. low levels of cr antibodies were found in the preinfection sera of only group ii ponies. upon inf ...19816260672
serological relationship between abortifacient and respiratory strains of equine herpesvirus type 1 in new zealand. 19816262693
herpesviral diseases affecting reproduction in the horse. 19806262988
investigations into fetal and neonatal losses in the horse. 19806262989
systemic diseases of the newborn foal. 19806262990
differentiation of sub-types of equine herpesvirus i by restriction endonuclease analysis. 19816266385
the diagnosis of viral disease in equine practice. 19816267764
differentiation of respiratory and abortigenic isolates of equine herpesvirus 1 by restriction endonucleases.viruses classified by immunologic criteria as equine herpesvirus 1 cause respiratory disease and abortion in horses. restriction endonuclease analyses of the dna's of viruses from animals with respiratory disease and from aborted fetuses show that the patterns for respiratory viruses, while similar to each other, are entirely different from the patterns for fetal viruses. it is therefore proposed that the dna restriction endonuclease patterns of fetal and respiratory viruses analyzed in this stu ...19816270790
structure of the genome of equine herpesvirus type 1. 19816270904
a radial immunodiffusion enzyme assay for detection of antibody to equine rhinopneumonitis virus (ehv-1) in horse serum.a radial immunodiffusion enzyme assay (ridea) was developed for detection and quantitation of antibodies to equine herpes virus-1 (ehv-1) in horse sera. the detection and quantitation of ehv-1 antibody levels were based on the diameter of the radial diffusion zone of specific antibody in each serum sample reacting with ehv-1 antigen. the circular zone was made visible using peroxidase-conjugated rabbit anti-horse immunoglobulin g and a substrate containing hydrogen peroxide. the results of the r ...19882847396
molecular cloning of equine herpesvirus type 1 dna: analysis of standard and defective viral genomes and viral sequences in oncogenically transformed cells.genomic dna sequences of equine herpesvirus type 1 (ehv-1) have been cloned as bamhi and ecori restriction fragments into the plasmid pbr322 and propagated in escherichia coli. with the exception of two ecori restriction fragments that reside in the s region of the viral genome, all of the cloned fragments demonstrated the same electrophoretic mobilities, restriction cleavage sites, and blot-hybridization patterns as did the parent fragments produced by bamhi or ecori digestion of virion dna. th ...19816273883
analysis of the genome of equine herpesvirus type 1: arrangement of cleavage sites for restriction endonucleases ecori, bglii and bamhi.the genome of an australian isolate of equine herpesvirus type 1 (equine abortion virus) has been analysed using the restriction endonucleases ecori, bglii and bamhi, and a physical map constructed. terminal fragments were identified by exonuclease treatments, and linkage of fragments was deduced by a combination of single- and double-digest experiments and cross-blot hybridizations. the genome has a mol. wt. of 100 x 10(6) and is comprised of a short unique region bounded by repetitive sequence ...19816275028
[comparative studies of the efficacy of paramunity inducer pind-avi, mitogen pha-p and rhinopneumonitis virus on peripheral leukocytes in the horse]. 19816275637
an outbreak of paresis in mares and geldings associated with equid herpesvirus outbreak of paresis occurred on a small isolated stud farm in july 1980. of the 42 horses on the stud, infection was confined to a group of nine in-foal mares and their foals and eight other horses which were either housed together at night or grazed adjacent pastures. eight mares and two geldings developed ataxia or paresis and one mare died. equid herpesvirus 1 was isolated from 17 animals and serological studies confirmed that 24 of 26 animals sampled had experienced infection.19816280366
serologic and molecular comparisons of several equine herpesvirus type 1 strains.the molecular and serologic relatedness of 2 recent respiratory tract isolates of equine herpesvirus type 1, designated t1 and t2, were compared with the army 183, kentucky-a hamster-adapted (kya-ha), and l-m cell-adapted (kya-lm) strains. electrophoresis in polyacrylamide gels revealed differences in virion structural proteins among 4 purified strains. seven envelope glycoproteins (molecular weight of 93,000, 65,000, 62,000, 60,000, 36,000, 20,000, and 18,000) corresponding to virion proteins 1 ...19816280520
equine herpesvirus type 1 (ehv-1) induced abortions and paralysis in a lipizzaner stud: a contribution to the classification of equine herpesviruses.out of 30 cases of abortion and perinatal deaths in a lipizzaner stud in austria 10 mares died after having shown central nervous system disturbances, ataxias and paralysis. the etiological agent of this "abortion storm" was equine herpesvirus type 1 (ehv-1). the restriction enzyme pattern of the dna from 5 isolates recovered from fetuses has been analyzed and compared with the known reference strains of ehv-1, -2, -4 and an austrian vaccine strain. the dna restriction profiles of the lipizzaner ...19863015084
characterization of the gene and an antigenic determinant of equine herpesvirus type-1 glycoprotein 14 with homology to gb-equivalent glycoproteins of other herpesviruses.the gene encoding glycoprotein 14 (gp14) of equine herpesvirus type 1 was sequenced. nucleotide sequence analysis revealed a complete transcription unit composed of a cat box, a tata box, a ribosome-binding sequence, a polyadenylation signal and an open reading frame (orf) of 2940 bp transcribed from left to right. the amino acid (aa) sequence deduced from this orf corresponded to that of a protein with 979 aa and had the characteristic features of membrane gp including a 20-aa signal sequence a ...19901692002
lymphocytes from ponies experimentally infected with equine herpesvirus 1: subpopulation dynamics and their response to mitogens.six pony foals, free of detectable serum neutralization (sn) antibody against equine herpesvirus type 1 by the standard virus-neutralization (vn) test, were inoculated with equine herpesvirus type 1. the ponies showed typical clinical signs of respiratory tract disease and developed a transient leukopenia, involving lymphocytes as well as neutrophils. the leukopenia reached its lowest point on postinoculation days (pid) 3 to 5 and then returned to base-line values by pid 8 to 10. on quantitation ...19826285778
equine influenza infections in great britain, 1979. 19826287694
latency of equine herpesvirus 4 (equine rhinopneumonitis virus) 19882849833
abortion and perinatal foal mortality associated with equine herpesvirus type 1 in a herd of grevy's zebra. 19862851577
rhinomune (rhinopneumonitis vaccine) 19826294955
a common code of practice for the control of contagious equine metritis and other equine reproductive diseases for the 1983 covering season in france, ireland and the united kingdom. 19826294959
enzyme-linked immunosorbent assay for the detection of antibodies to equid herpesvirus type 1 (ehv-1). 19863023732
variation in cellular tropism between isolates of equine herpesvirus-1 in foals.subtype-1 isolates of equine herpesvirus-1 (ehv-1) from a quadriplegic horse and from an aborted foetus were compared with each other and with a subtype-2 respiratory isolate. all 3 isolates were detected in the epithelium and macrophages of the respiratory tract. both the paresis and foetal subtype-1 isolates replicated in the epithelium of the ileum and this correlated with the recovery of virus from faeces in vivo. the paresis subtype-1 isolate also had a predelection for vascular endothelial ...19826297429
[creation of an avirulent and immunogenic mutant from the rhinopneumonitis virus].an avirulent immunogenic virus strain mutant of the causative agent of rhinopneumonia was found to cause abortions and respiratory diseases in horses. the mutant was obtained with the use of a virulent strain that induced strongly manifested clinical symptoms of the disease, and was cultured in cell media containing 5-iodine-2-desoxiuridine as an antimetabolite, following a definite pattern. it was found that the mutant completely lost its virulence, however, it retained its immunogenicity. it l ...19863024389
vaccines for ehv1. 19836298987
molecular epizootiologic studies of equine herpesvirus-1 infections by restriction endonuclease fingerprinting of viral dna. 19836299145
infectious center assay of intracellular virus and infective virus titer for equine mononuclear cells infected in vivo and in vitro with equine herpesviruses.a novel, simple method of infectious center assay was developed to detect and quantitate the intracellular existence of equine herpesvirus 1 and equine herpesvirus 2 in peripheral blood mononuclear cells infected in vivo and in vitro with the viruses by cocultivation of these cells with a permissive equine cell culture. the infectious center titers were correlated with the infectious virus titers. in vivo equine herpesvirus 1-infected mononuclear cells obtained from ponies experimentally infecte ...19836299486
application of a chemically inactivated, adjuvanted vaccine to control abortigenic infection of mares by equine herpesvirus i.a chemically inactivated, adjuvanted vaccine prepared from a virulent strain of equine herpesvirus i (ehv-i) was used to immunize pregnant thoroughbred broodmares during a five-year field test designed to determine its safety and efficacy. each mare in the vaccinated groups received 3 intramuscular injections of vaccine beginning immediately prior to and during the last half of pregnancy. vaccine was injected at approximately 60-day intervals. the accumulated incidence of ehv-i abortions among v ...19826299858
alterations in the equine herpesvirus 1 genome after in vitro and in vivo virus passage.the effect of in vitro and in vivo serial virus passage on the genetic stability of equine herpesvirus 1 (ehv-1) was investigated by restriction endonuclease analysis of the viral dna. dnas of ehv-1 isolates at different passage levels in cultured cells or in syrian hamsters were compared by electrophoresis of the dna cleavage fragments produced by restriction endonuclease digestion. no changes were observed in the restriction profile of the dnas of ehv-1 strains after 100 sequential passages in ...19836299965
genetic relatedness of the genomes of equine herpesvirus types 1, 2, and 3.genomic dnas of equine herpesvirus type 1 (ehv-1), ehv-2 (equine cytomegalovirus), and ehv-3 were examined by reassociation kinetic and thermal denaturation analyses to determine the extent and degree of homology among the three viral dnas. results of reassociation analyses indicated a limited homology among the three ehv genomes. homologous dna sequences equivalent to 1.8 to 3.7 megadaltons between ehv-1 and equine cytomegalovirus, 7.6 to 8.2 megadaltons between ehv-1 and ehv-3, and 1.3 to 1.9 ...19836300438
immunogenicity of equine herpesvirus type 1 (ehv1) and equine rhinovirus type 1 (erhv1) following inactivation by betapropiolactone (bpl) and ultraviolet (uv) light.some kinetic data on the inactivation of equine herpesvirus type 1 (ehv1) and equine rhinovirus type 1 (erhv1) by betapropiolactone (bpl) and ultraviolet (uv) irradiation are reported. 0.25% bpl at 37 degrees c for 1 h reduced the titre of ehv1 by greater than 10(3 . 4) and of erhv1 by greater than 10(4 . 1) tcid50/ml. uv irradiation (334 microw/cm2) produced similar reductions in titre after 2 min. these data were used as a basis for inactivating ehv1 and erhv1 by the combined action of bpl and ...19826301141
acute infections in young foals. 19836302008
vaccines for ehv1 abortion. 19836302971
vaccines for equine herpesvirus type 1. 19836304985
comparative efficacy of three 2'-fluoropyrimidine nucleosides and 9-(1,3-dihydroxy-2-propoxymethyl)guanine (bw b759u) against pseudorabies and equine rhinopneumonitis virus infection in vitro and in laboratory animals.the three 2'-fluoropyrimidine nucleosides 1-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)-5-iodocytosine (fiac), 1-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)-5-iodouracil (fiau), and 1-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)-5-methyluracil (fmau), showed high activity in rk13 monolayers against equine rhinopneumonitis virus, (ehv-1, ic50 range 0.02-0.18 microm), aujeszky's disease virus (shv-1, pseudorabies, ic50 range 0.25-7 microm) and infectious bovine rhinotracheitis virus (1br, bhv-1, ic50 ...19873026244
sensitivity of equine herpesviruses 1 and 3 in vitro to a new nucleoside analogue, 9-[[2-hydroxy-1-(hydroxymethyl) ethoxy] methyl] guanine.the following members of the herpetoviridae family were tested to determine their sensitivities to the new antiviral drug, biolf-62: equine herpesvirus types 1 and 3, human herpesvirus types 1 and 2, swine herpesvirus, bovine herpesvirus type 4, feline herpesvirus, canine herpesvirus, and herpes simiae virus. equine herpesviruses 1 and 3, human herpesviruses 1 and 2, and herpes simiae virus were all sensitive to biolf-62 at concentrations of less than 0.55 micrograms/ml. equine herpesvirus types ...19836307092
in vivo harboring of equine herpesvirus-1 in leukocyte populations and subpopulations and their quantitation from experimentally infected ponies. 19836309042
glycoprotein 300 is encoded by gene 28 of equine herpesvirus type 1: a new family of herpesvirus membrane proteins?a portion of equine herpesvirus type 1 (ehv-1) gene 28, which is homologous to herpes simplex virus type 1 gene ul32, was expressed using a prokaryotic system to yield a fusion protein which reacted on western blots with p19, a monoclonal antibody (mab) that reacts with ehv-1 glycoprotein 300 (gp300), confirming that this gene encodes gp300. hydrophobicity analysis showed that gp300 is a glycoprotein with multiple hydrophobic domains that might interact with, or span, the membrane several times. ...19921331295
chorioretinopathy associated with neuropathology following infection with equine herpesvirus-1. 19921332242
identification and control of the cis-acting elements of the immediate early gene of equid herpesvirus type 1.consensus cis-acting dna sequences upstream of the immediate early (ie) gene of equid herpesvirus type 1 (ehv-1, strain ab4) were identified. one copy of the conserved motif taatgarattc, which is the binding site for the host cellular factor oct-1 and herpes simplex virus type 1 (hsv-1) virion protein, vmw65, complex, was identified at positions -630 to -620. using transient transfections and chloramphenicol acetyltransferase assays the ie promoter of ehv-1 was shown to be trans-activated by vmw ...19921545217
a murine model for studying ehv-1-induced abortion.balb/c mice were infected with two abortigenic strains of equine herpesvirus-1 (ehv-1) by intranasal inoculation. the inoculation of one strain produced subclinical disease while the other produced a disease characterised by weight loss, constitutional signs, and death in the most severely affected animals. when pregnant mice were infected by the same method of inoculation, one strain of virus produced premature parturition; both strains produced fetal abnormalities. in some cases, virus could b ...19911654586
identification of cross-reacting glycoproteins of four herpesviruses by western blotting.monospecific rabbit antisera against purified herpes simplex virus type 1 (hsv-1) gb, gc or gd antigens or polyvalent rabbit antiserum against equine herpesvirus type 1 (ehv-1)-infected cells was used in western blotting to identify antigenically related proteins in cells infected with hsv-1, hsv-2, bovine mammillitis virus or ehv-1. monomeric and oligomeric polypeptides related to hsv-1 gb were found in cells infected with each of the four herpesviruses. the gc antiserum was specific for hsv-1 ...19852993488
comparison of stationary and roller cultures for the isolation of herpesviruses affecting livestock.the effect of rotating cultures on the isolation and cultivation of pseudorabies, infectious bovine rhinotracheitis and equine rhinopneumonitis viruses was studied. serial ten-fold dilutions of laboratory strains of these viruses and field strains of pseudorabies virus were inoculated in two each of roller and stationary tubes of appropriate cell cultures. all tubes were examined daily for the appearance of cytopathic effects. roller cultures were found to slightly enhance the speed and extent o ...19873035348
[equine herpesvirus 1 (ehv-1) infection in the horse: neurologic symptoms in a standard bred mare with acute fatal course. molecular characterization of the brain isolates and pathologic correlates]. 19873038071
kinetics of viral deoxyribonucleic acid, protein, and infectious particle production and alterations in host macromolecular syntheses in equine abortion (herpes) virus-infected cells.infection of exponential-phase suspension cultures of mouse fibroblast cells (l-m) with equine abortion virus (eav) resulted in inhibition of cell growth and marked alterations in host metabolic processes. the synthesis of deoxyribonucleic acid (dna) and ribonucleic acid was inhibited within 4 hr after infection and was suppressed by more than 90% by the time of maximal virus replication (14 to 18 hr). the overall rate of protein synthesis, however, was similar in uninfected and virus-producing ...19684302745
protein kinase activity in equine herpesvirus.a protein kinase which is intimately associated with equine herpesvirus (equine abortion virus) was found by using adenosine triphosphate-gamma-(32)p as a phosphate donor and virus protein as an acceptor. consistent demonstration of the activity requires prior removal of phosphohydrolase. the kinase activity requires mg(2+), is not stimulated by cyclic adenosine monophosphate, but is enhanced by added protamine or arginine-rich histone. the labeled product is resistant to ribonuclease, deoxyribo ...19724335515
equine abortion (herpes) virus: evaluation of markers in a field vaccination trial.twelve mares were vaccinated with attenuated equine abortion virus (eav) strain rac-h. two nonvaccinated mares served as controls. in at least three mares the vaccination appeared to coincide with a natural infection. this was indicated by characterization of the eav isolated from nasal secretions of six vaccinated mares, a nonvaccinated control, and also from the lung, spleen, and liver of a fetus aborted by a vaccinated mare. the relative sensitivity of the isolated eav to dithiothreitol was u ...19734796167
electron microscopy of equine respiratory viruses in organ cultures of equine fetal respiratory tract epithelium.organ cultures of equine fetal tracheal and nasal turbinate epithelium were inoculated with equine influenza virus-a1 (eiv-a1), equine herpesvirus-1 (ehv-1), or equine rhinovirus-1 (erv-1). infected organ cultures were processed for scanning and transmission electron microscopy at various intervals and were compared with noninfected controls. organ cultures inoculated with erv-1 appeared normal with the exception of rare island-like lesions in infected nasal turbinate. virus particles were not s ...19846093639
growth kinetics of equine respiratory tract viruses in cell and organ cultures.growth kinetics of equine influenza virus-a1, equine herpesvirus-1, and equine rhinovirus-1 were determined in susceptible cell monolayers and in organ cultures of equine fetal tracheal and nasal turbinate epithelium. equine influenza virus-a1 was replicated in cell and organ cultures and was released more readily and for longer periods from nasal turbinate epithelium than from tracheal epithelium. equine herpesvirus-1 was also replicated in cell and organ cultures. during the first 24 hours aft ...19846093640
functional and ultrastructural changes in neutrophils from mares and foals experimentally inoculated with a respiratory tract strain of equine herpesvirus-1.neutrophils isolated from venous blood of adult and foal ponies inoculated with equine herpesvirus-1 were evaluated by in vitro function tests and by electron microscopy. foals had fever and severe neutropenia 24 hours after inoculation; increased neutrophil random migration under agarose and decreased antibody-dependent cell-mediated cytotoxicity were significant at 24 hours, but values had returned to preinoculation levels by 72 hours. mares had fever and leukopenia of less severity, increases ...19846093641
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