Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| assessment of the base sequence homology between the two subtypes of equine herpesvirus 1. | the magnitude of the genetic relatedness of the two antigenic subtypes of equine herpesvirus 1 (ehv-1) was determined by dna-dna reassociation kinetics. denatured, labeled viral dna from one ehv-1 subtype was allowed to reassociate in the presence or absence of the unlabeled heterologous viral dna. the initial rate of reassociation of either labeled viral dna was increased by the presence of the heterologous viral dna to an extent indicating 10 to 20% homology between the two ehv-1 genomes. simi ... | 1982 | 6292488 |
| rhinomune (rhinopneumonitis vaccine) | 1982 | 6294955 | |
| a common code of practice for the control of contagious equine metritis and other equine reproductive diseases for the 1983 covering season in france, ireland and the united kingdom. | 1982 | 6294959 | |
| identification of the envelope surface glycoproteins of equine herpesvirus type 1. | the structural polypeptides of purified enveloped virions of the army 183 strain of equine herpesvirus type 1 (ehv-1) were examined by different analytical techniques to identify the envelope glycoproteins. glycoproteins were identified by electrophoretic analysis in polyacrylamide slab gels of virus labelled in vivo with [3h]glucosamine or labelled enzymically in vitro with either udp-[14c]galactose or sodium [3h]borohydride. fluorograms revealed eleven glycoproteins (mol. wt. 260000, 150000, 1 ... | 1982 | 6296290 |
| variation in cellular tropism between isolates of equine herpesvirus-1 in foals. | subtype-1 isolates of equine herpesvirus-1 (ehv-1) from a quadriplegic horse and from an aborted foetus were compared with each other and with a subtype-2 respiratory isolate. all 3 isolates were detected in the epithelium and macrophages of the respiratory tract. both the paresis and foetal subtype-1 isolates replicated in the epithelium of the ileum and this correlated with the recovery of virus from faeces in vivo. the paresis subtype-1 isolate also had a predelection for vascular endothelial ... | 1982 | 6297429 |
| immunogenicity of equine herpesvirus type 1 (ehv1) and equine rhinovirus type 1 (erhv1) following inactivation by betapropiolactone (bpl) and ultraviolet (uv) light. | some kinetic data on the inactivation of equine herpesvirus type 1 (ehv1) and equine rhinovirus type 1 (erhv1) by betapropiolactone (bpl) and ultraviolet (uv) irradiation are reported. 0.25% bpl at 37 degrees c for 1 h reduced the titre of ehv1 by greater than 10(3 . 4) and of erhv1 by greater than 10(4 . 1) tcid50/ml. uv irradiation (334 microw/cm2) produced similar reductions in titre after 2 min. these data were used as a basis for inactivating ehv1 and erhv1 by the combined action of bpl and ... | 1982 | 6301141 |
| application of a chemically inactivated, adjuvanted vaccine to control abortigenic infection of mares by equine herpesvirus i. | a chemically inactivated, adjuvanted vaccine prepared from a virulent strain of equine herpesvirus i (ehv-i) was used to immunize pregnant thoroughbred broodmares during a five-year field test designed to determine its safety and efficacy. each mare in the vaccinated groups received 3 intramuscular injections of vaccine beginning immediately prior to and during the last half of pregnancy. vaccine was injected at approximately 60-day intervals. the accumulated incidence of ehv-i abortions among v ... | 1982 | 6299858 |
| observations on vascular accidents in the central nervous system of neonatal foals. | a technique for the subarachnoid perfusion-fixation of the central nervous system was developed to help identify various significant vascular accidents (svas) in the central nervous system (cns) of 24 neonatal foals submitted for necropsy. svas, comprising subarachnoid, parenchymal and nerve root haemorrhages, and oedema and necrosis, occurred in 17 foals, more frequently in the spinal cord than the brain. they occurred as frequently in premature foals as in those born at full term, in foals bor ... | 1982 | 6962896 |
| ulcerative duodenitis in foals. | seven foals aged 18 days to 3 1/2 months had either single or multiple full-circumference segments or long antimesenteric bands of necrotizing duodenitis, sharply delineated from adjacent viable duodenum. perforation of the necrotic wall had occurred in all foals, leading to acute fibrinous peritonitis. on the mucosal surface severe diffuse, acute inflammation and ulceration involved the anterior half of the duodenum. two further foals, aged 28 and 30 days, had lesions that are believed to be a ... | 1983 | 6649336 |
| alterations in the equine herpesvirus 1 genome after in vitro and in vivo virus passage. | the effect of in vitro and in vivo serial virus passage on the genetic stability of equine herpesvirus 1 (ehv-1) was investigated by restriction endonuclease analysis of the viral dna. dnas of ehv-1 isolates at different passage levels in cultured cells or in syrian hamsters were compared by electrophoresis of the dna cleavage fragments produced by restriction endonuclease digestion. no changes were observed in the restriction profile of the dnas of ehv-1 strains after 100 sequential passages in ... | 1983 | 6299965 |
| genetic relatedness of the genomes of equine herpesvirus types 1, 2, and 3. | genomic dnas of equine herpesvirus type 1 (ehv-1), ehv-2 (equine cytomegalovirus), and ehv-3 were examined by reassociation kinetic and thermal denaturation analyses to determine the extent and degree of homology among the three viral dnas. results of reassociation analyses indicated a limited homology among the three ehv genomes. homologous dna sequences equivalent to 1.8 to 3.7 megadaltons between ehv-1 and equine cytomegalovirus, 7.6 to 8.2 megadaltons between ehv-1 and ehv-3, and 1.3 to 1.9 ... | 1983 | 6300438 |
| acute infections in young foals. | 1983 | 6302008 | |
| vaccines for ehv1 abortion. | 1983 | 6302971 | |
| vaccines for equine herpesvirus type 1. | 1983 | 6304985 | |
| [vaccination against the paralytic form of ehv1?]. | 1983 | 6306862 | |
| sensitivity of equine herpesviruses 1 and 3 in vitro to a new nucleoside analogue, 9-[[2-hydroxy-1-(hydroxymethyl) ethoxy] methyl] guanine. | the following members of the herpetoviridae family were tested to determine their sensitivities to the new antiviral drug, biolf-62: equine herpesvirus types 1 and 3, human herpesvirus types 1 and 2, swine herpesvirus, bovine herpesvirus type 4, feline herpesvirus, canine herpesvirus, and herpes simiae virus. equine herpesviruses 1 and 3, human herpesviruses 1 and 2, and herpes simiae virus were all sensitive to biolf-62 at concentrations of less than 0.55 micrograms/ml. equine herpesvirus types ... | 1983 | 6307092 |
| in vivo harboring of equine herpesvirus-1 in leukocyte populations and subpopulations and their quantitation from experimentally infected ponies. | 1983 | 6309042 | |
| equine immunology 4: vaccines and antisera. | this paper attempts to relate the practicalities of vaccine development to the ideals which should be aimed for in a new vaccine. the type of immune response induced is dependent upon the nature of the antigen in the vaccine and the site and timing of its presentation to the immune system. in this respect the influence of age, maternal immunity and antigenic competition are discussed. the possible side effects associated with vaccination are defined and vaccines which are currently available for ... | 1983 | 6309511 |
| outbreak of equine herpesvirus abortion in the hunter valley, new south wales. | 1983 | 6309512 | |
| location and orientation of homologous sequences in the genomes of five herpesviruses. | molecular hybridization experiments were carried out to investigate homologous regions in the genomes of herpes simplex virus types 1 and 2 (hsv-1 and hsv-2), equid herpesvirus 1 (ehv-1), pseudorabies virus (prv) and varicella-zoster virus (vzv). virion dna probes from ehv-1, prv and vzv hybridized to similar regions of the hsv genome, and the use of cloned dna probes allowed heterologous genomes to be oriented with respect to homologous regions. the hsv-1 and hsv-2 genomes are colinear, the ehv ... | 1983 | 6310033 |
| the pathogenicity in mice of respiratory, abortion and paresis isolates of equine herpesvirus-1. | eleven isolates of equine herpesvirus-1 (subtype 1) all infected the brain following intracerebral inoculation of 2 d.o. mice. most isolates were from cases of paresis, abortion or respiratory disease in the u.k., but established strains were also included. they divided into two subgroups. the 5 less pathogenic isolates were characterized by being restricted predominantly to the olfactory lobes. the 6 pathogenic isolates included the three known to cause equine paresis and were detected in neuro ... | 1983 | 6310851 |
| detection of equine herpesvirus-1 antigen and the specific antibody by enzyme-linked immunosorbent assay. | enzyme-linked immunosorbent assays (elisa) were developed to detect equine herpesvirus-1 (ehv-1) antigens and specific antibodies. detection of ehv-1 antigens was done by a 4-layer elisa. in inoculated cell cultures, ehv-1 cell antigen was detected after postinoculation (pi) hour 4, reached approximately twice the value of ehv-1 viral antigen (extra-cellular virus) in pi hour 18, and peaked in pi hour 24, whereas ehv-1 viral antigen appeared after pi hour 12, increased steadily, and peaked highe ... | 1983 | 6314855 |
| relative activities of acyclovir and bw759 against aujeszky's disease and equine rhinopneumonitis viruses. | compound bw759 (9-[2-hydroxy-1-(hydroxymethyl)ethoxymethyl]guanine) was shown to be about 230 times more active than acyclovir (9-[2-hydroxyethoxymethyl]guanine) (acv) against equid herpesvirus type 1 infection in syrian hamsters and was more effective against aujeszky's disease in mice. the therapeutic superiority of bw759 over acv was greater than expected from quantitative inhibitory results in tissue culture with these viruses. when administered to hamsters at dose rates sufficient to preven ... | 1983 | 6314886 |
| restriction endonuclease dna fingerprinting of respiratory, foetal and perinatal foal isolates of equine herpesvirus type 1. | dna was prepared from 43 equine herpesvirus type 1 (ehv 1) isolates, 11 of which were from horses with respiratory disease, 22 from aborted equine foetuses, and 10 from foals that died perinatally. the restriction endonuclease dna fingerprints of 10 of the 11 respiratory isolates, known with certainty to have been recovered from horses with respiratory disease, were entirely different from all but 3 of the 32 foetal or perinatal foal isolates. the exceptional respiratory isolate, ehv 1 army 183, ... | 1983 | 6314938 |
| equine herpesvirus abortion in australia 1977 to 1982. | until 1977 no case of abortion caused by equine herpesvirus 1 (ehv1) had been recorded in australia although the virus, called equine rhinopneumonitis virus, had been known to have been present at least since 1962. outbreaks of ehv1 abortion occurred in new south wales in 1977 and in 1981. sporadic cases of ehv1 abortion had been confirmed in some parts of australia each year since 1975. it was concluded that an abortigenic subtype of ehv1 had been introduced to australia in 1977 and that the pr ... | 1983 | 6315394 |
| a common code of practice for the control of contagious equine metritis and other equine reproductive diseases for the 1984 covering season in france, ireland and the united kingdom. | 1983 | 6318420 | |
| a specific viral dna sequence is stably integrated in herpesvirus oncogenically transformed cells. | 1983 | 6297800 | |
| vaccines for ehv1. | 1983 | 6298987 | |
| molecular epizootiologic studies of equine herpesvirus-1 infections by restriction endonuclease fingerprinting of viral dna. | 1983 | 6299145 | |
| infectious center assay of intracellular virus and infective virus titer for equine mononuclear cells infected in vivo and in vitro with equine herpesviruses. | a novel, simple method of infectious center assay was developed to detect and quantitate the intracellular existence of equine herpesvirus 1 and equine herpesvirus 2 in peripheral blood mononuclear cells infected in vivo and in vitro with the viruses by cocultivation of these cells with a permissive equine cell culture. the infectious center titers were correlated with the infectious virus titers. in vivo equine herpesvirus 1-infected mononuclear cells obtained from ponies experimentally infecte ... | 1983 | 6299486 |
| antibody to neuritogenic myelin protein p2 in equine paresis due to equine herpesvirus 1. | 1983 | 6190332 | |
| immunologic relationships between equine herpesvirus type 1 (equine abortion virus) and type 4 (equine rhinopneumonitis virus). | the specificity of selected immune responses to equine herpesvirus type 1 (ehv-1) and type 4 (ehv-4) was examined in 3 colostrum-deprived specific-pathogen-free foals. single foals were vaccinated with inactivated ehv-1, inactivated ehv-4, or control cell lysate plus adjuvant followed by successive intranasal challenge exposures with ehv-1 and ehv-4 or with ehv-4 and ehv-1. vaccination with inactivated virus preparations elicited cellular immune responses and antibody which were augmented by sub ... | 1984 | 6208822 |
| the antiviral spectrum of (e)-5-(2-bromovinyl)-2'-deoxyuridine. | the antiviral activity spectrum of (e)-5-(2-bromovinyl)-2'-deoxyuridine (bvdu) is not restricted to herpes simplex virus type 1 (hsv-1) and varicella-zoster virus (vzv) but also encompasses several other herpesviruses such as suid herpesvirus type 1 (shv-1), bovid herpesvirus type 1 (bhv-1), simian varicella virus (svv), herpesvirus saimiri, herpesvirus platyrrhinae, and the baculovirus trichoplusia ni multiple nuclear polyhedrosis virus. other herpesviruses such as herpes simplex virus type 2, ... | 1984 | 6092320 |
| vaccination against equine herpesvirus 1 and equine influenza infection. | 1984 | 6093330 | |
| electron microscopy of equine respiratory viruses in organ cultures of equine fetal respiratory tract epithelium. | organ cultures of equine fetal tracheal and nasal turbinate epithelium were inoculated with equine influenza virus-a1 (eiv-a1), equine herpesvirus-1 (ehv-1), or equine rhinovirus-1 (erv-1). infected organ cultures were processed for scanning and transmission electron microscopy at various intervals and were compared with noninfected controls. organ cultures inoculated with erv-1 appeared normal with the exception of rare island-like lesions in infected nasal turbinate. virus particles were not s ... | 1984 | 6093639 |
| growth kinetics of equine respiratory tract viruses in cell and organ cultures. | growth kinetics of equine influenza virus-a1, equine herpesvirus-1, and equine rhinovirus-1 were determined in susceptible cell monolayers and in organ cultures of equine fetal tracheal and nasal turbinate epithelium. equine influenza virus-a1 was replicated in cell and organ cultures and was released more readily and for longer periods from nasal turbinate epithelium than from tracheal epithelium. equine herpesvirus-1 was also replicated in cell and organ cultures. during the first 24 hours aft ... | 1984 | 6093640 |
| functional and ultrastructural changes in neutrophils from mares and foals experimentally inoculated with a respiratory tract strain of equine herpesvirus-1. | neutrophils isolated from venous blood of adult and foal ponies inoculated with equine herpesvirus-1 were evaluated by in vitro function tests and by electron microscopy. foals had fever and severe neutropenia 24 hours after inoculation; increased neutrophil random migration under agarose and decreased antibody-dependent cell-mediated cytotoxicity were significant at 24 hours, but values had returned to preinoculation levels by 72 hours. mares had fever and leukopenia of less severity, increases ... | 1984 | 6093641 |
| pathogenicity of equine herpesvirus 1 subtype 2 for foals and adult pony mares. | three pony mares and 4 pony foals were inoculated with a subtype 2 strain of equine herpesvirus 1. foals had periods of fever 12 h and 2.5 days after inoculation and leukopenia, involving both neutrophils and lymphocytes, followed by leukocytosis. mares had transient fever and leukopenia 24 hours after inoculation that were less severe than in foals. an increase in circulating virus-neutralizing antibody was seen in 2 of 3 inoculated mares, but not in foals. attempts to isolate virus from blood ... | 1984 | 6095515 |
| [neurological disorders caused by equine herpesvirus type 1 and cauda equina neuritis in horses]. | the differences in aetiology, symptomatology, pathomorphology, diagnosis and therapy between the nervous form (paralytic form) of equine herpes virus, type 1, and neuritis caudae equinae are reviewed. the conclusion is that in most cases it is possible to differentiate between these two clinical syndromes. | 1984 | 6096984 |
| specificity of pseudorabies virus serotests. | pigs experimentally inoculated with bovine herpesvirus-1 or equine herpesvirus-1 developed mild clinical disease signs. regression of clinical disease was accompanied by development of specific virus-neutralizing antibodies. these antibodies did not react positively with pseudorabies antigens in the serum-virus neutralization test, an indirect radioimmunoassay, or a microimmunodiffusion test. | 1984 | 6098206 |
| [current virus diseases in horses. diseases in foals and respiratory tract infections]. | at the moment, horse praxis is confronted by two disease complexes which are difficult to fight against as well in prophylaxis as in therapy, but which get an increasing importance. first they concern virus infections of the foals and second primary virus-caused respiratory diseases. foals get infected during the embryonal/fetal development, in the perinatal or postnatal period. normally the infection is caused by latent infected, clinical healthy mares, or in the postnatal period by ubiquitous, ... | 1984 | 6098971 |
| molecular epidemiology and pathogenesis of some equine herpesvirus type 1 (equine abortion virus) and type 4 (equine rhinopneumonitis virus) isolates. | representative strains of ehv isolated from an aborted foetus and from a horse with rhinopneumonitis in new zealand had restriction endonuclease dna fingerprints typical of those usually associated with these syndromes elsewhere and now designated ehv1 and 4 respectively. ehv1 was isolated from the brain and spinal cord of a 4-year-old gelding that died of myeloencephalitis. a mare on the same farm, at about the same time as the gelding developed myeloencephalitis, aborted and ehv1 was isolated ... | 1984 | 6099117 |
| structure and genetic complexity of the genomes of herpesvirus defective-interfering particles associated with oncogenic transformation and persistent infection. | the complexity and structural organization of defective-interfering (di) particle dna of equine herpesvirus type 1 (ehv-1) have been elucidated by using restriction enzyme and southern blot hybridization analyses. di particles were generated by serial high-multiplicity passage of ehv-1 in l-m cells, and total viral dna was extracted from virus purified from supernatants of these serial passages. ehv-1 di particle dna was quantitatively separated from standard (std) dna by several cycles of cscl ... | 1984 | 6321784 |
| [the neurological form of evh1 in horses]. | 1984 | 6322380 | |
| coestablishment of persistent infection and oncogenic transformation of hamster embryo cells by equine cytomegalovirus. | semipermissive, primary hamster embryo (he) cells were morphologically transformed in vitro by infection with uv-irradiated equine cytomegalovirus (equine herpesvirus type 2; ecmv). cell lines (designated ec-1-3) were established independently from foci and were shown to exhibit growth and biological properties typically associated with transformed cells: altered morphology, loss of contact inhibition, increased saturation density, decreased generation time, immortality in culture, normal growth ... | 1984 | 6322417 |
| structure of the genome of equine herpesvirus type 3. | restriction endonuclease mapping studies were performed to determine the molecular structure of the genome of equine herpesvirus type 3 (ehv-3). purified ehv-3 dna, either unlabeled or 32p-labeled, was analyzed using the restriction enzymes bamhi, bcli, bglii, ecori, and hindiii. the findings that four 0.5 m (molar) fragments were present, that two of these were terminal fragments, and that all 0.5 m fragments contained homologous dna sequences as judged by dna hybridization analyses indicated t ... | 1984 | 6322418 |
| equine cytomegalovirus: structural proteins of virions and nucleocapsids. | enveloped virions and nucleocapsids of equine cytomegalovirus (ecmv; equine herpesvirus type 2) have been purified from the supernatants and the nuclear extracts of infected rabbit kidney (rk) cells, respectively, and their structural protein compositions have been analyzed. sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis revealed that ecmv nucleocapsids were composed of nine proteins (average molecular weights = 148k, 52k, 49.5k, 46k, 43.5k, 38.5k, 27k, 20k, and 18k), which t ... | 1984 | 6324468 |
| trials of an inactivated equid herpesvirus 1 vaccine: challenge with a subtype 1 virus. | vaccinated yearlings , two-year-old and in-foal pony mares with appropriate controls were exposed to aerosols of a subtype 1 virus one to two months after two or three vaccinations; all became infected. no obvious differences in the febrile responses, clinical signs and subsequent abortions were found between vaccinated and control mares. all vaccinated yearlings and two-year-old ponies developed a febrile respiratory disease but this was less severe than that suffered by the controls and the am ... | 1984 | 6328728 |
| trials of an inactivated equid herpesvirus 1 vaccine: challenge with a subtype 2 virus. | serological responses following two and three doses of an inactivated equid herpesvirus 1 ( ehv -1) vaccine containing a subtype 1 strain were examined in yearling ponies. complement fixing antibody responses were significantly higher against the subtype 1 vaccine strain than against a subtype 2 virus. complement fixing antibody responses declined rapidly after the second dose of vaccine and had returned to almost pre-vaccination levels eight weeks after the second dose of vaccine. complement fi ... | 1984 | 6328729 |
| antigenic and biochemical analysis of gb of herpes simplex virus type 1 and type 2 and of cross-reacting glycoproteins induced by bovine mammillitis virus and equine herpesvirus type 1. | an antiserum was produced to the oligomeric form of glycoprotein b (gb) induced by herpes simplex virus type 1 (hsv-1) strain 17. this antiserum gave a single common precipitin line in agar gel immunodiffusion with hsv-1, hsv-2, bovine mammillitis virus (bmv) and equine herpesvirus type 1 (ehv-1). it also neutralized hsv-1, hsv-2 and bmv but not ehv-1. absorption of the antiserum with excess hsv-2 or bmv antigen resulted in an hsv-1-specific neutralizing antiserum. in immunoprecipitation, two pr ... | 1985 | 2981966 |
| a new field strain of equine abortion virus (equine herpesvirus-1) among kentucky horses. | from restriction endonuclease characterization of the dna of 317 isolates of equine abortion virus (equine herpesvirus-1; ehv-1) from 176 epizootically unrelated outbreaks of equine virus abortion occurring over 24 years in kentucky, an epizootic pattern and variation of the virus have emerged. two electropherotypes of ehv-1 (1p and 1b) accounted for greater than 90% of the nonvaccine-related abortion isolates examined. from 1960 to 1981, ehv-1 1p was the predominant isolate circulating in the c ... | 1985 | 2982294 |
| the effect of 2'-fluoro-2'-deoxycytidine on herpes virus growth. | the effect of 2'-fluoro-2'-deoxycytidine (dcfl) on the growth of certain viruses of the herpes type was investigated. it is shown that the compound has considerable anti-viral activity against hsv-i, hsv-ii, pseudorabies virus and equine abortion virus. it has an effect comparable to that of arac and is more efficient than br5dc, but less so than acyclovir. experiments with thymidine kinase-negative strains of hsv-i indicated that dcfl was phosphorylated by the viral kinase, and its km appears t ... | 1985 | 2982405 |
| inclusions in equine cytologic specimens. | inclusions and cellular changes were seen in cytologic specimens from 1 healthy horse, 6 horses hospitalized because of respiratory problems, and 1 horse hospitalized because of colic and hepatitis. two bronchial aspirates contained detached ciliated cytoplasmic tufts and cytoplasmic inclusions characteristic of the specific degenerative process called ciliocytophthoria. these changes and inclusions resembled those seen in bronchial aspirates from human beings with parainfluenza virus infection. ... | 1985 | 2982775 |
| [ehv1, to vaccinate or not?]. | 1985 | 2984805 | |
| endoscopic and virological observations on respiratory disease in a group of young thoroughbred horses in training. | a group of racehorses in training was examined on several occasions with a fibreoptic endoscope and monitored for viral infection. only equine herpes virus-2 (ehv-2) infection was detected. pharyngeal lymphoid hyperplasia (plh) was present in all horses and decreased in severity with age. there was no association between plh severity and antibody titres to ehv-1, or with the isolation of ehv-2. finishing position in races was not affected by plh severity. exercise induced pulmonary haemorrhage ( ... | 1985 | 2985380 |
| rapid subtyping of equine herpesvirus 1 with monoclonal antibodies. | two antigenically similar subtypes of equine herpesvirus 1 (ehv-1) cause disease in horses. a procedure for rapid differentiation of the two ehv-1 subtypes with monoclonal antibodies was developed. subtype-specific pools of monoclonal antibodies were constructed, characterized, and used in enzyme immunofiltration and indirect immunofluorescence assays to subtype 50 epizootiologically unrelated field isolates of ehv-1. both assays allowed accurate subtype identification of each ehv-1 isolate with ... | 1985 | 2987299 |
| equine herpesvirus type 1 infected cell polypeptides: evidence for immediate early/early/late regulation of viral gene expression. | ehv-1 polypeptide synthesis was examined in productively infected rabbit kidney and hamster embryo cells. sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) analyses of extracts from [35s]methionine- and 3h-amino acid-labeled-infected and mock-infected cultures revealed the presence of 30 infected cell-specific polypeptides (icps) which ranged in apparent molecular weights from 16.5k to 213k. twenty-two of these icps comigrated with virion structural proteins. four icps (203k, ... | 1985 | 2990102 |
| identification of cross-reacting glycoproteins of four herpesviruses by western blotting. | monospecific rabbit antisera against purified herpes simplex virus type 1 (hsv-1) gb, gc or gd antigens or polyvalent rabbit antiserum against equine herpesvirus type 1 (ehv-1)-infected cells was used in western blotting to identify antigenically related proteins in cells infected with hsv-1, hsv-2, bovine mammillitis virus or ehv-1. monomeric and oligomeric polypeptides related to hsv-1 gb were found in cells infected with each of the four herpesviruses. the gc antiserum was specific for hsv-1 ... | 1985 | 2993488 |
| a comparison of the acid-soluble polypeptides of five herpesviruses. | the polypeptides soluble in 0.25 m-hcl were extracted from the nuclei of bhk cells infected with herpes simplex virus type 1 or type 2 and separated by sds-page. seventeen polypeptides were detectable in each extract of which 10 type 1 and nine type 2 polypeptides were reproducibly effectively extracted. in cells infected with bovine mammillitis virus, pseudorabies virus or equine herpesvirus type 1, at least 12, 13 and eight polypeptides respectively were acid-soluble. in addition to histones, ... | 1985 | 2995559 |
| equid herpesvirus 1 (ehv 1) latency: more questions than answers. | 1985 | 2996877 | |
| experimental reactivation of equid herpesvirus 1 (ehv 1) following the administration of corticosteroids. | eight ponies were experimentally infected with equid herpesvirus 1 (ehv 1) (subtype 1). all animals showed clinical and serological evidence of infection and virus was isolated from nasal swabs and leucocytes. these ponies were kept in isolation for a further three months during which time complement fixing antibody decreased at least four-fold. following immunosuppression with dexamethasone and prednisolone subtype 1 virus was recovered from six of the eight animals within 14 days. five of thes ... | 1985 | 2996879 |
| neurologic diseases. | the responses of apparently healthy newborn foals to neurologic testing differ significantly from those of adult horses. these responses and the diagnostic techniques pertinent to neurologic problems are reviewed as a basis for evaluation of the compromised neonatal foal. the more frequently encountered neurologic diseases are discussed in a problem-oriented format. these clinical problems include behavioral abnormalities, convulsions, changes in consciousness, blindness, ataxia without loss of ... | 1985 | 3000543 |
| equine herpesvirus type 1 abortion in an onager and suspected herpesvirus myelitis in a zebra. | 1985 | 3001004 | |
| isolation of equine herpesvirus 1 from the brain of a horse affected with paresis. | 1985 | 3002312 | |
| selected animal herpesviruses: new concepts and technologies. | 1985 | 3004157 | |
| [application of the passive hemagglutination test to the study of equine rhinopneumonitis. i. serologic studies in rabbits inoculated with equine herpesvirus type 1]. | 1985 | 3016853 | |
| antibodies against equine herpesvirus 1 in the cerebrospinal fluid in the horse. | neutralizing antibodies against equine herpesvirus 1 were measured in serum and cerebrospinal fluid of 16 horses and ponies from a closed herd both before and after vaccination with modified live equine herpesvirus 1. these titers were also measured in 22 neurologically normal and 15 neurologically abnormal horses at a teaching hospital. animals from the closed herd had prevaccination serum titers up to 1:8 and postvaccination serum titers up to 1:128. horses from the teaching hospital had serum ... | 1985 | 17422553 |
| equine herpesvirus type 1 (ehv-1) infection and vaccination with an inactivated ehv-1 vaccine. | 1986 | 16031215 | |
| viruses associated with respiratory disease of horses in new zealand: an update. | viruses causing or associated with respiratory disease in horses worldwide are reviewed. results are presented from a serological survey of 121 new zealand foals and horses that had been affected by respiratory disease, determining the prevalence of antibodies in this country to the major viruses associated with similar disease overseas. to date there is no evidence of equine influenza virus in new zealand. both equine herpesvirus type 1 and 2 have been frequently isolated and show high serologi ... | 1986 | 16031261 |
| equine herpesvirus type 1 infection--a reply. | 1986 | 16031308 | |
| molecular pathogenesis of equine coital exanthema: temperature-sensitive function(s) in cells infected with equine herpesviruses. | preliminary experiments have revealed that several laboratory and wild-type strains of the equine herpesvirus (ehv) triad were temperature-sensitive for growth when assayed at 39 degrees c. the efficiencies of plating (eop) observed were 10(-2) for both ehv 1 and 2, and 1 x 10(-6) for ehv 3. the eops were determined by plaque assays which compared titrations at 34 degrees c and 39 degrees c on equine fetal dermal fibroblast cells. growth yield experiments, assayed at 34 degrees c, reflected thos ... | 1986 | 3016974 |
| cloning and fine mapping the dna of equine herpesvirus type one defective interfering particles. | equine herpesvirus type one (ehv-1) defective interfering (di) particle dna fragments were inserted into the xbai site of the plasmid vector pacyc184. five di xbai fragments, which ranged in molecular weight from 4.5 to 6.7 mda, were selected for detailed analysis. each di dna clone was labeled with 32p-deoxynucleotides by nick translation and hybridized to genomic digests of ehv-1 standard (std) dna bound to nitrocellulose. all five clones were shown to hybridize to dna sequences derived from t ... | 1986 | 3016989 |
| equine herpesvirus genomes: heterogeneity of naturally occurring type 4 isolates and of a type 1 isolate after heterologous cell passage. | the restriction endonuclease dna fingerprints of 20 low passage, epidemiologically unrelated isolates of equine herpesvirus 4 (equine rhinopneumonitis virus) showed considerable heterogeneity in certain fragments, the positions of which were assigned to quite restricted positions on the 141 kilobase (kb) genome. we note that the heterogeneity observed in the restriction endonuclease dna fingerprints of ehv 1 (equine abortion virus) and of pseudorabies virus also tend to map to these same restric ... | 1986 | 3022687 |
| enzyme-linked immunosorbent assay for the detection of antibodies to equid herpesvirus type 1 (ehv-1). | 1986 | 3023732 | |
| [creation of an avirulent and immunogenic mutant from the rhinopneumonitis virus]. | an avirulent immunogenic virus strain mutant of the causative agent of rhinopneumonia was found to cause abortions and respiratory diseases in horses. the mutant was obtained with the use of a virulent strain that induced strongly manifested clinical symptoms of the disease, and was cultured in cell media containing 5-iodine-2-desoxiuridine as an antimetabolite, following a definite pattern. it was found that the mutant completely lost its virulence, however, it retained its immunogenicity. it l ... | 1986 | 3024389 |
| rapid method for the identification and screening of herpesviruses by dna fingerprinting combined with blot hybridization. | rapid characterization of herpesvirus isolates exemplified by equine herpesviruses is described. total dnas were isolated from virus infected small scale cell cultures. the dna fragments obtained after restriction enzyme digestion were separated on agarose gels, transferred and immobilized on filter membranes. a radioactively labelled probe derived from the purified dna of an ehv-1 reference strain was used for hybridization in order to detect the restriction fragments of different ehv-1 field i ... | 1986 | 3025240 |
| [application of the passive hemagglutination test to the study of equine rhinopneumonitis. ii. serologic study in horses]. | 1986 | 3035677 | |
| molecular epizootiology, pathogenesis, and prophylaxis of equine herpesvirus-1 infections. | 1986 | 2856183 | |
| heat-inactivated frog virus 3 selectively inhibits equine herpesvirus type 1 translation in a temporal class-dependent manner. | superinfection of equine herpesvirus type 1 (ehv-1)-infected rabbit kidney cells with heat-inactivated frog virus 3 (delta fv3) differentially blocked ehv-1 protein synthesis. the extent of inhibition varied with the specific ehv-1 message, but in general late protein synthesis was inhibited more than early and immediate early translation. since fv3 has been shown to block heterologous rna and protein synthesis, it was necessary to determine whether the observed reduction in herpesvirus protein ... | 1986 | 3727402 |
| genetic relatedness and colinearity of genomes of equine herpesvirus types 1 and 3. | the arrangement and location of homologous dna sequences within the genomes of equine herpesvirus type 1 (ehv-1) and ehv-3 were investigated by using southern blot hybridization analyses conducted under stringent conditions. recombinant plasmid libraries comprising 95 and 84% of the ehv-1 and ehv-3 genomes, respectively, were labeled with 32p-deoxynucleotides by nick translation and were used as probes in filter hybridization studies. the dna homology between the ehv-1 and ehv-3 genomes was disp ... | 1986 | 3005628 |
| genomic and antigenic comparison of an equine herpesvirus 1 (ehv 1) isolate from the 1983 lippizan abortion storm with ehv 1 reference strains. | an ehv 1 isolate from the lippizan stud at piber, which caused the abortion and paresis outbreak in 1983, was investigated using 3 known subtype 1 and 2 subtype 2 strains for comparison. broad-scale restriction enzyme analysis as well as cross-neutralization with hyperimmune sera produced in rabbits were performed, and sds-page of infected cell proteins was conducted on a limited scale. the piber isolate was clearly classified as a subtype 1 strain of ehv 1, and showed closest resemblance in its ... | 1986 | 3012290 |
| specific inhibition of herpesvirus ribonucleotide reductase by synthetic peptides. | ribonucleotide reductase is an essential enzyme for dna synthesis in all prokaryotic and eukaryotic cells; it catalyses the reductive conversion of ribonucleotides to deoxyribonucleotides. several herpesviruses including herpes simplex virus type 1 (hsv-1), hsv-2, pseudorabies virus (prv), equine herpesvirus type 1 (ehv-1) and epstein-barr virus (ebv) have been found to induce novel ribonucleotide reductase activities. there is evidence that the hsv-1 ribonucleotide reductase activity is virus-e ... | 1986 | 3012359 |
| endothelial cell infection and thrombosis in paralysis caused by equid herpesvirus-1: equine stroke. | eight mares were infected with equid herpesvirus-1 subtype 1 isolated from a case of equine paresis. in two mares killed at 4 d.p.i. immunofluorescence showed endothelial cell infection together with thrombosis in the rete arteriosus of the nasal mucosa and also in the spinal cord of one of these mares. circulating platelet counts in the other six mares fell as early as 2 d.p.i. and remained depressed for seven days. circulating immune complexes started to appear at 2 d.p.i., reached maximum lev ... | 1986 | 3015074 |
| equine herpesvirus type 1 (ehv-1) induced abortions and paralysis in a lipizzaner stud: a contribution to the classification of equine herpesviruses. | out of 30 cases of abortion and perinatal deaths in a lipizzaner stud in austria 10 mares died after having shown central nervous system disturbances, ataxias and paralysis. the etiological agent of this "abortion storm" was equine herpesvirus type 1 (ehv-1). the restriction enzyme pattern of the dna from 5 isolates recovered from fetuses has been analyzed and compared with the known reference strains of ehv-1, -2, -4 and an austrian vaccine strain. the dna restriction profiles of the lipizzaner ... | 1986 | 3015084 |
| foetal and neonatal foal losses on equine herpesvirus type 1(ehv-1) infected farms before and after ehv-1 vaccination was introduced. | on 6 farms with a history of ehv-1 abortions the overall prevalence of losses due to abortion, stillbirth and neonatal foal mortality during 5 foaling seasons preceding the introduction of vaccination against ehv-1 were compared with corresponding data for 8 subsequent seasons in which all horses were immunized with a modified live ehv-1 vaccine. although both sporadic and epizootic ehv-1 abortions occurred in immunized mares, the numbers of foetal and neonatal foal losses decreased significantl ... | 1986 | 2830601 |
| abortion and perinatal foal mortality associated with equine herpesvirus type 1 in a herd of grevy's zebra. | 1986 | 2851577 | |
| innate immunity during equid herpesvirus 1 (ehv-1) infection. | intrinsic phagocytosis and killing of c. albicans by equine monocytes and polymorphonuclear leucocytes (pmn) was examined during equid herpesvirus 1 (ehv-1) (subtypes 1 or 2) and adenovirus infections. monocyte function increased during ehv-1 subtype 2 and adenovirus infection. conversely, there was an impairment of monocyte ingestion during ehv-1 subtype 1 infection which was ascribed to virus replication in peripheral blood mononuclear cells. pmn phagocytosis was not decreased in any of the in ... | 1986 | 2431815 |
| rapid diagnosis and characterization of equid herpesvirus 1 using monoclonal antibodies. | twelve monoclonal antibodies (mabs) were produced against the attenuated rach subtype 1 strain of equid herpesvirus 1. nine were subtype specific by immunofluorescence or immunoperoxidase, while the other three were against epitopes common to both subtypes. use of these mabs allowed isolates to be rapidly and easily subtyped. immunoblotting indicated that the subtype common mabs were against viral proteins of mw 140 k and 90 k (vp 9 and 14), while the subtype-specific mabs were against the glyco ... | 1987 | 2438808 |
| demonstration of equine herpes virus 1 (ehv-1) in histological sections and tissue cultures by the peroxidase-antiperoxidase (pap) technique. | 1987 | 2830744 | |
| neuropathogenicity for suckling mice of equine herpesvirus 1 from the lipizzan outbreak 1983 and of selected other ehv 1 strains. | 1987 | 2830747 | |
| [new observations in the diagnosis of ehv (equine herpes virus) abortions]. | over the last years the percentage of foals lost by ehv at term or close to term seems to be higher than in former years. furthermore, the pathological findings seem to shift from liver to lung. so far there has been no explanation for this phenomenon. | 1987 | 2831640 |
| changes in restriction enzyme pattern of the equine herpes virus type 1 (ehv-1) strain rac h dna during attenuation. | 1987 | 2823507 | |
| serological and virological investigations of an equid herpesvirus 1 (ehv-1) abortion storm on a stud farm in 1985. | an extensive outbreak of ehv-1 abortions occurred on a stud farm in england in 1985. of the 67 pregnant mares present on the stud farm, 31 were challenged with ehv-1, resulting in the loss of 22 fetuses or foals. laboratory investigations revealed that the spread of the virus closely followed movement of apparently healthy mares (during the incubation period of the infection). during the outbreak mares were challenged 1-4 months before the expected foaling date. there was no relationship between ... | 1987 | 2824770 |
| plasma concentrations of progestagens, oestrone sulphate and prolactin in pregnant mares subjected to natural challenge with equid herpesvirus-1. | multiparous pregnant mares, on two studfarms, were studied following natural challenge with equid herpesvirus-1 (ehv-1). they were divided into three groups according to serum complement fixation titres: group a (n = 11) were not challenged and delivered normal foals; group b (n = 13) were challenged but delivered normal foals; group c (n = 23) were challenged and delivered infected foals which were stillborn or lived for less than 31 h. in groups a and b mean (+/- s.d.) gestational age at deliv ... | 1987 | 2824771 |
| characterization and mapping of equine herpesvirus type 1 immediate early, early, and late transcripts. | northern blot analysis was used to characterize and map equine herpesvirus type 1 (ehv-1) immediate early (ie), early, and late transcripts. genomic ehv-1 dna and cloned ehv-1 restriction endonuclease fragments, representing the entire genome, were 32p-labeled and hybridized to immobilized total cell rna isolated from ehv-1 infected rabbit kidney cells incubated in the presence or absence of metabolic inhibitors. a single 6.0 kilobase (kb) ie transcript mapped to viral inverted repeat sequences. ... | 1987 | 2825444 |
| differential metabolism of (e)-5-(2-iodovinyl)-2'-deoxyuridine (ivdu) by equine herpesvirus type 1- and herpes simplex virus-infected cells. | thymidine kinase (tk) enzymes encoded by herpes simplex viruses types 1 and 2 (hsv-1, hsv-2), and equine herpesvirus type 1 (ehv-1) catalyze the phosphorylation of thymidine (dthd) and (e)-5-(2-bromovinyl)-2'-deoxyuridine (bvdu). the replication of hsv-1 is sensitive to bvdu, but the replication of hsv-2 and ehv-1 is not. to investigate the differential sensitivity of the viruses to halogenated vinyldeoxyuridine drugs, the phosphorylation of 125i-labeled (e)-5-(2-iodovinyl)-2'-deoxyuridine (ivdu ... | 1987 | 2825591 |
| genetic restriction of cytolysis during equid herpesvirus 1 subtype 2 infection. | six welsh mountain pony foals were experimentally infected with a subtype 2 isolate of equid herpesvirus 1 (ehv-1) and subsequently examined for t cell mediated cytotoxicity against both subtypes. cytotoxicity was not observed at 3 or 7 days after primary exposure but virus-specific, and genetically restricted, cytotoxicity of ehv-1-labelled autologous skin fibroblasts could be demonstrated 7 and 21 days after the animals were given a second exposure to live virus. killing of subtype 2 antigen-l ... | 1987 | 2827921 |
| characterization of two equine herpesvirus (ehv) isolates associated with neurological disorders in horses. | 1987 | 2829471 | |
| a suspected case of equine herpesvirus 1 encephalomyelitis. | 1987 | 2833882 | |
| the proteins of equid herpesvirus 1 (ehv 1) recognised by equine antisera and their ability to promote antibody-dependent cell-mediated cytotoxicity. | equine sera were used to immunoprecipitate radiolabelled virus-infected cell proteins; subsequent resolution with polyacrylamide gel electrophoresis identified the ehv-1 polypeptides vp 2, 10a, 11, 13, 14, 15, 16, 20, 21 and 23a. the humoral support of adcc by these sera was examined in vitro. cytotoxicity could be demonstrated against both subtypes irrespective of the immunising isolate. the implications of these results are discussed. | 1987 | 3035747 |