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the genome of bovine herpesvirus 1 (bhv-1) strains exhibiting a neuropathogenic potential compared to known bhv-1 strains by restriction site mapping and cross-hybridization.bovine herpesvirus 1 (bhv-1) strains can be differentiated by their dna and polypeptide patterns, and by antigenic properties as demonstrated by monoclonal antibodies. we classified the bhv-1 strains according to these data as bhv-1.1, bhv-1.2 (a/b) and bhv-1.3 (a/b). bhv-1.1 and bhv-1.2 correspond to the well known 'common' bhv-1 strains, whereas bhv-1.3 has only recently been recognized and exhibits a neuropathogenic potential. in the present paper we describe the structural genome characteris ...19863026111
isolation of a herpesvirus serologically related to bovine herpesvirus 1 from a reindeer (rangifer tarandus). 19863026156
restriction endonuclease patterns of bovine herpesvirus type 1 isolated from bovine mammary glands.restriction endonuclease analysis was used, in conjunction with viral neutralization and growth-curve experiments, to compare a bovine herpesvirus type 1 (bhv-1) isolate, originally obtained from bovine mammary gland lesions, with a standard bhv-1 strain, infectious bovine rhinotracheitis virus. although differences were not detected by viral neutralization or growth-curve experiments, restriction fragment patterns generated by bam hi, eco ri, hind iii, and hpa i, revealed definite differences b ...19863026212
comparative efficacy of three 2'-fluoropyrimidine nucleosides and 9-(1,3-dihydroxy-2-propoxymethyl)guanine (bw b759u) against pseudorabies and equine rhinopneumonitis virus infection in vitro and in laboratory animals.the three 2'-fluoropyrimidine nucleosides 1-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)-5-iodocytosine (fiac), 1-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)-5-iodouracil (fiau), and 1-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)-5-methyluracil (fmau), showed high activity in rk13 monolayers against equine rhinopneumonitis virus, (ehv-1, ic50 range 0.02-0.18 microm), aujeszky's disease virus (shv-1, pseudorabies, ic50 range 0.25-7 microm) and infectious bovine rhinotracheitis virus (1br, bhv-1, ic50 ...19873026244
intracellular localization and transport of three different bovine herpesvirus type 1 glycoproteins involved in neutralization.monoclonal antibodies against 3 different glycoproteins of bovine herpesvirus type 1 (bhv-1) involved in virus neutralization were used in indirect immunofluorescence (iif) tests to characterize the appearance and transport to the plasma membrane of virus antigens in the infected cells. antibodies against gp 117 and gp 71 glycoproteins first showed pronounced ring-like nuclear fluorescence at 4 hours post-infection (pi), followed by staining of the perinuclear region, presumably the golgi appara ...19873026288
an evaluation of five serological tests for the detection of antibody to bovine herpesvirus 1 in vaccinated and experimentally infected cattle.more than 300 bovine sera from a previously reported vaccination and challenge trial were tested for antibodies to bovine herpesvirus 1 (bhv1) by five serological assays: enzyme-linked immunosorbent assay (elisa) for igm and igg, passive haemagglutination (pha), and two methods of virus neutralisation (vn). in a statistical comparison of elisa (igg), pha and vn results, the assays showed highly significant correlations (p less than 0.01). the sensitivities of elisa and 24-hour neutralisation tes ...19863027800
[bvd (bovine virus diarrhea) and ibr (infectious bovine rhinotracheitis): serologic studies on 35 farms].1099 female cattle from 35 farms in the provinces of north brabant, gelderland and overijssel were examined for the presence of antibodies against bovine virus diarrhoea (bvd) and infectious bovine rhinotracheitis (ibr) by serum neutralisation tests. one of the farms examined was seronegative for bvd and 11 farms were seronegative for ibr. on the seropositive farms about 50% of cattle carrying their first calf had not experienced infection i.e. were seronegative (bvd 51.2%, ibr 48.3%). after cal ...19863027917
patterns of bovine t cell-mediated immune responses to bovine herpesvirus 1.lymphocyte proliferation was used to evaluate t cell-mediated immune responses to different isolates of bovine herpesvirus 1 (bhv-1). groups of high, moderate, and low responses were observed when lymphocytes from three breeds of dairy cattle were stimulated with each of the bhv-1 isolates. proliferation of cells in the low responding group could be augmented by exogenous il-2. the mechanism of unresponsiveness by cells from one individual whose response was not altered by il-2 supplementation w ...19863029942
natural cell-mediated cytotoxicity to cells infected with infectious bovine rhinotracheitis virus.cell-mediated cytotoxicity against viral-infected cells was demonstrated in a 6-hr 51cr release assay. peripheral blood mononuclear leukocytes from both infectious bovine rhinotracheitis virus (ibrv)-infected and noninfected cattle exhibited preferential lysis against ibrv-infected primary bovine embryonic kidney (bek) cells compared to cells infected with pseudorabies virus and noninfected bek cells. addition of specific antibody to the assay did not enhance cytotoxicity. the effector cell was ...19873029944
[infectious bovine rhinotracheitis. kinetics of antibody in a dairy herd]. 19863030026
[development and course of ibr antibody titers after vaccination with bovigrip plus]. 19873030258
[suitability of the immunodiffusion test for determining humoral antibodies to bovine herpesvirus (bhv 1)]. 19863030261
inheritance and interaction of immune traits in beef calves.three sets of blood samples were obtained from beef calves of two experimental populations and assayed for various immunological measurements. the first set of samples was taken between 24 and 48 h after birth and quantified for igg1 concentration. a second set was taken immediately prior to vaccination for infectious bovine rhinotracheitis virus (ibrv; at an average age of 164 d) and a third set taken 60 d post-vaccination. these later samples were quantified for antibodies specific to ibrv. le ...19873030992
hemagglutination inhibition and virus neutralizing response of rabbits inoculated with bovine herpesvirus 1 subunit vaccine.the immunogenicity of bovine herpesvirus type 1 (bhv-1) hemagglutinin has been investigated. both live and nonionic detergent solubilized vaccines were prepared and 5000 hemagglutinating units (hau) were injected subcutaneously into rabbits. both types of vaccine induced a good antibody response but live virus was four times more efficient in inducing hemagglutination inhibiting and neutralizing antibodies than either triton x-100- or octylglucoside-solubilized subunit vaccine. blotting analysis ...19873031871
highly sensitive antigen detection procedures for the diagnosis of infectious bovine rhinotracheitis: amplified elisa and reverse passive haemagglutination.the sensitivity of an enzyme-linked immunosorbent assay (elisa) for the detection of bovid herpesvirus 1 antigen was increased by up to 50-fold using the biotin-avidin interaction to amplify the reaction, when compared with a simple sandwich elisa. an alternative immunoassay, reverse passive haemagglutination (rpha), had a similar sensitivity to the amplified elisa, and was technically simpler to perform. both the amplified elisa and the rpha could detect viral antigen in the nasal secretions of ...19873031873
reactivation in calves of latent infection by bovid herpesvirus-4.nine calves, six of which had been infected with strain 85/bh 16tv and three with strain 85/bh 232tn of bovid herpesvirus-4 (bhv-4), were treated with dexamethasone (dms) three months after infection. dms administration did not induce any clinical signs of disease, but bhv-4 was isolated from the nasal swabbings of all calves for a maximum of 8 days after the start of dms treatment. the virus was also isolated from the nerve tissues, nasal mucosa, lymph nodes, lung and spleen of 4 calves that we ...19873033450
[freeze-etching study of the replication of the infectious bovine rhinotracheitis virus]. 19863033912
reactivity of monoclonal antibodies to proteins of a neurotropic bovine herpesvirus 1 (bhv-1) strain and to proteins of representative bhv-1 strains.15 monoclonal antibodies (mcabs) were induced with the neuropathogenic strain n 569 of bovine herpesvirus 1 type 3 (bhv-1.3). nine of them could be shown by radioimmunoprecipitation assay to react with viral glycoproteins and two of these mcabs were able to neutralize strain n 569. the reactivity of these 15 monoclonals was compared with 11 monoclonal antibodies induced with a bhv-1.1 strain. the available monoclonal antibodies made it possible to characterize bhv-1.3 and to classify bhv-1 into ...19873034198
reactivation of infectious bovine rhinotracheitis virus by transport.transport was studied as a cause of reactivation of infectious bovine rhinotracheitis virus (bovine herpesvirus-1; bhv-1) in heifers vaccinated 2-6 months before transport, using a double dose of the thermosensitive (ts) vaccine strain (tracherine). eight out of 19 animals showed ts strain re-excretion over a period of 1-3 days, beginning, in 5 out of the 8 heifers, the day after transport. in 14 other heifers, only sera were examined by sero-neutralisation: only 1 out of these 14 animals showed ...19873034502
comparison of stationary and roller cultures for the isolation of herpesviruses affecting livestock.the effect of rotating cultures on the isolation and cultivation of pseudorabies, infectious bovine rhinotracheitis and equine rhinopneumonitis viruses was studied. serial ten-fold dilutions of laboratory strains of these viruses and field strains of pseudorabies virus were inoculated in two each of roller and stationary tubes of appropriate cell cultures. all tubes were examined daily for the appearance of cytopathic effects. roller cultures were found to slightly enhance the speed and extent o ...19873035348
viral-bacterial pneumonia in calves: effects on plasma eicosanoids and long chain fatty acids.in the pathogenesis of bovine pneumonic pasteurellosis, immunodepression induced by stress or respiratory viral infection permits superinfection of the lungs with pasteurella hemolytica, which results in exudative fibrinous pneumonia. therefore, bovine pneumonic pasteurellosis was induced by sequential inoculations of calves with bovine herpes virus-1 (bhv-1, 3 x 10(7) tissue culture infectious dose 50 (tcid50)/nostril), followed 3 days later by challenge with p. hemolytica (15 x 10(9) colony-fo ...19873036736
the genome of caprine herpesvirus 1: genome structure and relatedness to bovine herpesvirus 1.caprine herpesvirus 1 (caphv-1) dna was examined by electron microscopy, restriction site mapping and homology studies with bovine herpesvirus 1 (bhv-1) dna. although the restriction site maps differed significantly, we showed that the genome structures of caphv-1 and bhv-1 were identical and that the dnas shared a high degree of base sequence homology.19873037021
first isolation of ibr virus in turkey. 19873037743
protection of cattle from bovine herpesvirus type i (bhv-1) infection by immunization with individual viral glycoproteins.the major glycoproteins gi, giii, and giv of bovine herpesvirus-1 (bhv-1) were found to induce high levels of antibody in cattle which could neutralize virus and participate in antibody-dependent cell cytotoxicity of bhv-1-infected cells. immunized animals were fully protected from disease, using a bhv-1/pasteurella haemolytica aerosol challenge model but not from infection with the virus. thus, virus could still replicate in the nasal passages of immunized animals, although to a lesser extent t ...19873037783
detection of bovine herpesvirus-1 nucleic acid sequences, using a dot-blot hybridization procedure.a sensitive and specific procedure for the detection of argentine isolates of bovine herpesvirus-1 was developed. the procedure was based on a dot-blot, nucleic acid hybridization, using 32p, nick-translated, plasmidic probes. the probes contained cloned bam h1 restriction fragments in the left half of the viral genome. the detection limit of the procedure was 10 pg of viral dna.19873037953
use of recombinant bovine alpha 1 interferon in reducing respiratory disease induced by bovine herpesvirus type 1.intranasal or intramuscular treatment of calves with recombinant bovine alpha 1 interferon before challenge with bovine herpesvirus type 1 and pasteurella haemolytica reduced clinical signs, number of sick days, lung lesions, and weight loss. the effective dose was determined to be relatively broad within the range of 1 to 50 mg per animal. no adverse effects were observed even at high doses of interferon (50 mg per animal). administration before virus infection was more effective than administr ...19873038008
elisa for bovine herpesvirus 1 (bhv1) antibody with hirt supernatant.detailed studies were made of the ability of hirt supernatant (hs) from bovine herpesvirus 1 (bhv1) infected cultures to sensitize plates for enzyme-linked immunosorbent assay (elisa). the ultracentrifuged pellet of hs had less sensitizing activity than the supernatant, but the antigen was removed completely by 0.22 micron filters and to some extent by 0.45 micron filters; it was minimally affected by sonication; it was destroyed by the action of pronase but not by dnaase i when a kallikrein ina ...19873038916
[bhv-1 infection of cattle: stabilization by active immunization of seropositive animals with inactivated vaccine--a field trial]. 19873040371
comparison of five cell lines for the propagation of bovine viral diarrhea and infectious bovine rhinotracheitis viruses.a comparative study was carried out to determine the susceptibility of 5 different cell types to bovine viral diarrhea (bvd) and infectious bovine rhinotracheitis (ibr) viruses. the cell systems used were swine testicle (st), mink lung (ml), bovine turbinate (bt), porcine kidney (pk15) and equine dermal (ed) cells. for bvd virus, the titers obtained on day 8 post-infection were 10(1.13), 10(3.25), 10(4.13), 10(0.00) and 10(0.00), in st, ml, bt, pk15 and ed cells, respectively. for the ibr virus, ...19873041184
[evaluation of synthetic activity of lymphocyte nucleoli and phagocytic activity in peripheral blood leukocytes in heifers after infection with infectious bovine rhinotracheitis virus].the biosynthetic activity of the nucleoli of peripheral blood lymphocytes in calves, and the proportion of lymphocytes with the micronucleoli, with compact and ring-shaped nucleoli, were evaluated after experimental intranasal and intratracheal infections with the infectious bovine rhinotracheitis (ibr) virus. ibr virus belongs to the group of herpes viruses, the control of which is dominated by cellular immunity. the results are compared with the values for phagocytizing neutrophils, phagocytic ...19873122402
prevalence and specificity of antibodies to bovine respiratory syncytial virus in sera from feedlot and range cattle.the specificity of serum antibodies for the polypeptides of bovine respiratory syncytial virus (brsv) was examined, using sera obtained from feedlot and range cattle. test results in sera from feedlot cattle indicated a 60% rate of seroconversion and 95% seropositivity to brsv, associated with lack of clinical signs indicative of respiratory tract disease. exposure to other common respiratory tract viruses also was high (greater than or equal to 92% to bovine herpesvirus type 1, bovine viral dia ...19883178027
reproductive difficulties in cattle with antibody titers to haemophilus somnus.a herd of holstein cows was examined because of suspected embryonic death. four cows had embryonic loss, and 2 cows had aborted. paired serum samples were tested for antibodies to infectious bovine rhinotracheitis virus, bovine viral diarrhea virus, and haemophilus somnus. of 16 cows, 8 had antibody titers to h somnus greater than 1:1,024, and 3 had greater than or equal to four-fold changes in antibody titers to h somnus. haemophilus somnus infection was active in this herd and may have been re ...19883192475
nitrogen kinetics of infectious bovine rhinotracheitis-stressed calves.eight crossbred feeder steers were used in two consecutive n balance studies to investigate the effects of infectious bovine rhinotracheitis virus (ibrv) on n kinetics. balance study 1, which followed a 10-d acclimation phase, consisted of 7 d of sample collection referred to as the healthy phase (d -7 to d -1). study 2, the ibrv-infected phase, began 2 d after a nasal ibrv challenge and continued for 6 d (d 2 to d 8). a stable isotope, [15n]-glycine, was used to determine n kinetics in both stu ...19883209506
control of infectious bovine rhinotracheitis in calves with a bhv-1 subunit-iscom vaccine.an iscom subunit vaccine was prepared by adsorption of purified viral membrane proteins of bhv-1 on the glycoside quil a and assayed in six-month-old seronegative calves. groups of five animals were given three intramuscular doses of 50 or 25 micrograms bhv-1/iscoms or a commercial attenuated vaccine. both types of vaccine induced seroconversion: the iscom vaccine consistently gave a serological response superior to that of the attenuated vaccine with maximal titres of 1/608 in haemagglutination ...19883245294
activation of protein breakdown and prostaglandin e2 production in rat skeletal muscle in fever is signaled by a macrophage product distinct from interleukin 1 or other known monokines.during sepsis or after injection of endotoxin into rats, there is a large increase in muscle protein breakdown and prostaglandin e2 (peg2) production. prior studies showed that partially purified interleukin 1 (il-1) from human monocytes can stimulate these processes when added to isolated rat muscles. the availability of pure recombinant il-1 and other monokines has allowed us to investigate the identity of the active agent in this process. incubation of muscles with recombinant human or murine ...19883284911
efficacy of a streptomycin-dependent, live pasteurella haemolytica vaccine against challenge exposure to pasteurella haemolytica in cattle.a streptomycin-dependent, live pasteurella haemolytica vaccine was given in 1 or 2 doses to 2 groups of weaned calves; 2 other groups of calves were not vaccinated. all calves in the vaccinated groups and calves in 1 of the nonvaccinated groups were stressed by transport, intratracheally inoculated with bovine herpesvirus type-1 (cooper strain), and then intratracheally inoculated with p haemolytica type a1. the 4th group of calves (nonvaccinated controls) was not stressed and were not intratrac ...19873296883
vaccination studies against experimental bovine pasteurella pneumonia.vaccination-challenge experiments were conducted in colostrum-deprived calves to evaluate the efficacy of pasteurella bacterins and vaccines against experimental pneumonic pasteurellosis. calves were vaccinated with formalin-killed bacterins and live vaccines, then challenge exposed intratracheally with p. haemolytica or p. multocida. infectious bovine rhinotracheitis virus was inoculated intranasally three to four days prior to p. haemolytica challenge-exposure. all calves were examined for mac ...19873300921
validation of disease diagnoses reported to the national animal health monitoring system from a large colorado beef feedlot.clinical observation and collection of biological specimens from a large beef feedlot (approximately 30,0000 animals) were used to evaluate 6 approaches for validation of a disease reporting system. data collected during a 12-month period were used to evaluate each approach. a subsample of disease cases reported to the national animal health monitoring system (nahms) was compared with the clinical observations of the investigators. although the agreement between clinical diagnosis by the nahms v ...19883372333
early embryonic death in heifers after inoculation with bovine herpesvirus-1 and reactivation of latent virus in reproductive tissues.thirteen crossbred heifers seronegative for bovine herpesvirus-1 (bhv-1) were bred naturally to a seronegative bull. eight heifers were inoculated with bhv-1, iv, on postbreeding day (pbd) 7 or 14. viremia was detected in heifers 1 through 7, and virus also was isolated from nasal and vaginal secretions of heifers 2, 3, 4, 6, and 7. the pregnancy status of all heifers was monitored from pbd 14 to pbd 35 by determining plasma progesterone concentrations at 1- to 3-day intervals. decreased progest ...19873434897
cytotoxicity of bovine lymphocytes after treatment with lymphokines.cytotoxic lymphocytes were generated from bovine peripheral blood mononuclear leukocytes after in vitro stimulation with lymphokines that contained interleukin-2. lymphokine-stimulated cultures were cytotoxic to k562 cells (human natural killer [nk] targets) and yac-1 cells (mouse nk targets), but not to hsb-2 cells (human nk targets) in a 4-hour, 51cr-release assay. cells generated after lymphokine activation also mediated antibody-dependent cellular cytotoxicity to hsb-2 cells. appearance of e ...19863488701
bovine peripheral blood leukocyte subpopulation dynamics following a primary bovine herpesvirus-1 infection.population dynamics of bovine peripheral blood leukocyte subpopulations were quantitated following a primary bovine herpesvirus-1 (bhv-1) infection. percoll isolated peripheral blood mononuclear cell (pbmc) subpopulations were analyzed using flow cytometry (fc) and cytochemical stains. between days two to eight post-infection (pi) there was a significant decrease in the percentage of t-cells and nont/nonb cells which was accompanied by an increased percentage of b-cells and monocytes. these perc ...19873509948
t lymphocyte population dynamics and function following a primary bovine herpesvirus type-1 infection.following a primary bovine herpesvirus-1 (bhv-1) infection the concanavalin a (con a) induced proliferative responses of peripheral blood t lymphocytes were suppressed. this suppression occurred in the absence of detectible serum suppressor factors, suppressor cell activity or decreased accessory cell function. however, regression analysis demonstrated a significant correlation between the percentage of t lymphocytes present within the peripheral blood mononuclear cell (pbmc) population and the ...19873509949
diagnosis of bovine respiratory syncytial virus infections improved by virus detection in lung lavage samples.the potential of lung lavage to diagnose and study infections with bovine respiratory syncytial virus (brsv) was investigated. the technique was simple to perform under field conditions, and even in calves with respiratory tract disease, serious drawbacks were not observed. in 9 epizootics of respiratory tract disease, brsv infection was diagnosed in 1 or more calves. the brsv was detected from a total of 21 of 32 calves by immunofluorescence in cells recovered from lavage fluids, and the virus ...19863511803
effects of infectious bovine rhinotracheitis virus infection on bovine airway reactivity.the response of isolated tracheal and bronchial strips to isoproterenol in vitro was studied in eleven male jersey calves. clinical, microbiological and pathological evaluations of the calves were carried out. in calves exposed once or twice to infectious bovine rhinotracheitis virus, the relaxation threshold of the trachealis muscle to isoproterenol was significantly (p less than 0.05) impaired (threshold 5.0 x 10(-7) m, single exposure and 1.0 x 10(-7) m, double exposure), when compared with u ...19873651887
effect of dexamethasone on bovine leukocyte functions and bovine herpesvirus type-1 replication.in an attempt to elucidate the mechanism whereby dexamethasone could reactivate bovine herpesvirus type-1 the effect of dexamethasone on virus replication and leukocyte functions was assessed. no effect was detectable on either virus yield or in vitro replication kinetics. in contrast, dexamethasone influenced several leukocyte functions thought to be of importance in antiviral defense and maintenance of latency. in vitro exposure of peripheral blood polymorphonuclear neutrophilic granulocytes o ...19873651888
vaccines for respiratory disease in cattle.respiratory disease is one of the most serious disease complexes affecting beef cattle production. for example, it is claimed to cost the uk industry about 70 million pounds per year. it is usually associated with young cattle and can occur in a variety of situations. it is a good example of multifactorial disease in that its aetiology involves both infection by a variety of microorganisms and a number of environmental factors. several distinct syndromes occur and a number of microorganisms are ...19873673201
study on the etiologic role of bovine respiratory syncytial virus in pneumonia of dairy calves.bovine respiratory syncytial virus (brsv) was the viral agent most commonly identified in 14 epizootics of pneumonia in dairy calves. a microtiter serum-virus neutralization test proved to be the best means of identifying involvement of brsv; seroconversion (fourfold or greater rise in titer) was demonstrated in 10 of the 14 epizootics. only limited involvement of bovine viral diarrhea virus, infectious bovine rhinotracheitis virus, parainfluenza 3 virus, and bovine adenovirus type 3 was recogni ...19863733504
colonization of the nasal passages of calves with pasteurella haemolytica serotype 1 and regeneration of colonization after experimentally induced viral infection of the respiratory tract.healthy nonstressed calves were inoculated intranasally with or subjected to aerosol exposure to pasteurella haemolytica serotype 1. only 4 of 28 calves harbored the bacterium in enough numbers to be isolated from the nasal passages for more than 7 days. after apparent clearing from the nasal passages, 8 calves were inoculated intranasally with infectious bovine rhinotracheitis virus; 2 of these calves shed the p haemolytica during clinical illness due to the virus. the remaining 20 calves were ...19863752678
systemic neonatal infectious bovine rhinotracheitis virus infection in suckler calves. 19863776068
serologic survey of viral antibodies in the peruvian alpaca (lama pacos).sera from more than 100 alpacas (lama pacos) from the peruvian southern sierra were examined for antibodies to 8 viruses known to infect other domestic animals. on the basis of these serologic findings and previously published serologic or clinical data, it is now known that the alpaca can be infected with the following viruses: parainfluenza-3, bovine respiratory syncytial virus, bovine herpesvirus-1, bluetongue virus, border disease virus, influenza a virus, rotavirus, rabies virus, vesicular ...19873826854
clinical and experimental modifications of plasma iron and zinc concentrations in cattle.plasma iron and zinc concentrations were studied in pyrexic cattle or in cattle experimentally infected with infectious bovine rhinotracheitis virus or escherichia coli endotoxin. plasma iron and zinc levels tended to decline in the animals given endotoxin and in the pyrexic cattle, but the plasma iron level was only modified after experimental infectious bovine rhinotracheitis infection. these changes were not always related to pyrexia. plasma iron and zinc concentrations taken together may be ...19853890352
preliminary studies with a live streptomycin-dependent pasteurella multocida and pasteurella haemolytica vaccine for the prevention of bovine pneumonic pasteurellosis.twelve pasteurella-free holstein-friesian calves were used in a study to test the efficacy of a live streptomycin-dependent pasteurella multocida a:3 and streptomycin-dependent pasteurella haemolytica a1 vaccine. the calves were inoculated intramuscularly twice at 14-day intervals with either the streptomycin-dependent vaccine, containing 1 x 10(6) colony forming units/ml p. multocida and 4 x 10(8) colony forming units/ml p. haemolytica, commercial bacterin, or phosphate buffered saline. two wee ...19853907804
serum complement-fixing antibody to pasteurella haemolytica a1 and its effect on experimentally induced pneumonic pasteurellosis in calves.ninety-three calves comprising 16 experimental groups were exposed to viral (bovine herpesvirus-1 or parainfluenza-3 virus) and pasteurella haemolytica aerosols. serum samples from these calves were tested before and after exposure for antibodies to p haemolytica by a modified direct complement-fixation test. at slaughter of the calves, the extent of pneumonia produced was estimated for each calf and compared with the results of the modified direct complement-fixation tests. the extent of pneumo ...19853909862
parasites, diseases, and health status of sympatric populations of fallow deer and white-tailed deer in kentucky.in august 1983, a study on parasites, diseases, and health status was conducted on sympatric populations of fallow deer (dama dama) and white-tailed deer (odocoileus virginianus) from land between the lakes, lyon and trigg counties, kentucky. five adult deer of each species were studied. white-tailed deer had antibodies to epizootic hemorrhagic disease (ehd) virus and leptospira interogans serovariety icterohemorrhagiae, and fallow deer had antibodies to bluetongue and ehd viruses. serologic tes ...19853999248
morphometric studies of the bovine uterus: microscopic lesions and retrospective reproductive history.a survey of reproductive tracts from nonpregnant cows at an abattoir was conducted. reproductive tracts from 98 cows were studied microscopically and evaluated with a grading system to determine the severity of pathologic changes. inflammatory reaction (type and distribution), fibrosis, gland morphology, and appearance were measured, quantified, and scored (1 to 4). category 1 endometrium (normal) was found in 18 cows, category 2 in 23, category 3 in 34, and category 4 in 23. the category of end ...19854083598
effects of hydroxyurea on replication of infectious bovine rhinotracheitis virus. 19704099191
intracellular localization and sequential development of infectious bovine rhinotracheitis viral antigens. 19714108774
[experimental infections of the central nervous system caused by bovine rhinotracheitis virus]. 19654284146
a quantitative study of the infectious bovine rhinotracheitis neutralization test. 19654285515
an indirect hemagglutination test for detecting antibody to infectious bovine rhinotracheitis virus. 19654285755
the activity of immuno-conglutinin in in vitro viral infections. 19654286431
studies of infectious bovine rhinotracheitis virus. ii. effect of metabolic inhibitors on virus multiplication. 19654286662
infectious bovine rhinotracheitis abortion and its relationship to nutrition in california beef cattle. 19654287014
viral causes of bovine abortion in ohio. 19654287330
infectious bovine rhinotracheitis--clinical, pathological, and virological aspects. 19664287716
a case of infectious bovine rhinotracheitis. 19664287903
mixed infections or contaminations of bovine viral diarrhea virus with infectious bovine rhinotracheitis virus. 19664287980
infectious bovine rhinotracheitiis--sudden death in feedlot cattle. 19664288095
susceptibility of goats to infectious bovine rhinotracheitis. 19664288510
immunization programs for dairy cattle. 19664289588
survey of bovine respiratory diseases with special reference to the serological examination of paired serum samples. 19664289917
placental pathology. v. placental lesions of cattle experimentally infected with infectious bovine rhinotracheitis virus. 19664290879
the pathogenesis of encephalitis caused by the infectious bovine rhinotracheitis virus. 19664290885
reactivation of ether-inactivated infectious bovine rhinotracheitis virus. 19674291078
isolation and characterization of a strain of infectious bovine rhinotracheitis virus associated with enteritis in cattle: isolation, serologic characterization, and induction of the experimental disease. 19664291326
isolation and characterization of a strain of infectious bovine rhinotracheitis virus associated with enteritis in cattle: comparative developmental study by fluorescent antibody tracing and electron microscopy. 19664291327
isolation and characterization of a strain of infectious bovine rhinotracheitis virus associated with enteritis in cattle: pathogenesis studies by fluorescent antibody tracing. 19664291328
a microbiological study of bovine conjunctivitis and keratoconjunctivitis. 19674291465
influence of relative humidity on the survival of some airborne viruses.a system for studying the effects of relative humidity (rh) and temperature on biological aerosols, utilizing a modified toroid for a static aerosol chamber, is described. studies were conducted at 23 c and at three rh levels (10, 35, and 90%) with four viruses (newcastle disease virus, infectious bovine rhinotracheitis virus, vesicular stomatitis virus, and escherichia coli b t3 bacteriophage). virus loss on aerosol generation was consistently lower at 90% than at 10 or 35% rh. when stored at 2 ...19674291670
the effect of artificial insemination with semen contaminated with ibr-ipv virus. 19674291681
viral respiratory infection in commercial feedlot cattle. 19674291850
the growth of mycoplasma bovigenitalium in cell cultures. 19674291869
plaque characteristics of four classes of bovine viruses. 19674292079
infectious bovine rhinotracheitis--a summary of the disease and its control with emphasis on new zealand conditions. 19674292389
the virus of infectious bovine rhinotracheitis in northern nigeria. 19664292451
abortion in cattle caused by experimental infection with infectious bovine rhinotracheitis virus. 19674292796
comparison of selected characteristics of four strains of infectious bovine rhinotracheitis virus. 19674293273
infectious bovine rhinotracheitis-infectious pustular vulvovaginitis virus infection in pregnant cows. 19674293274
inactivation of infectious bovine rhinotracheitis virus by calcium alginate wool swabs. 19674293387
comparative virology of the herpes group. 19674293689
the structure of infectious bovine rhinotracheitis virus. 19664294185
immunogenicity of bovine rhinotracheitis--virus diarrhea vaccine in cattle inoculated simultaneously with concentrated homologous antiserum. 19674294704
application of the agar gel technique in the diagnosis of infectious bovine rhinotracheitis. 19674294769
a histological study of the effects of the herpesvirus of infectious bovine rhinotracheitis in the lactating bovine mammary gland.mastitis was produced in three first calf heifers by inoculation of the udder with the virus of infectious bovine rhinotracheitis. these animals were killed at different times during the course of the disease and tissues were harvested and examined histologically. the basic lesion resulted from the necrotizing effect of the virus upon the epithelial cells of the alveoli and ducts. eosinophilic intranuclear inclusion bodies were demonstrated in tissues from animals killed at three and six days po ...19674295256
[experimental infection of the testis with rhinotracheitis (ibr) virus]. 19674295719
[incidence and distribution of antibodies against causal agents of respiratory infections in the serum of cattle in italy. ii. serum neutralization of infectious rhinotracheitis virus]. 19664295774
the effect of 5-iodo-2'-deoxyuridine on the multiplication of infectious bovine rhinotracheitis virus. 19684296236
complications in cattle following vaccination with a combined bovine viral diarrhea-infectious bovine rhinotracheitis vaccine. 19684297313
long term maintenance of cell cultures under agar overlay and development of herpes t plaques. 19684297881
[a simple method for the enrichment of virus in tissue culture fluid by adsorption on calcium phosphate]. 19664298117
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