anti-herpesvirus activity of carbocyclic oxetanocin g in vitro.a series of new compounds, carbocyclic oxetanocins, have been synthesized and their anti-herpesvirus activity determined. carbocyclic oxetanocin g (oxt-g) was most active against herpes simplex virus (hsv) and human cytomegalovirus (hcmv) among carbocyclic oxetanocins tested; the median effective concentrations (ec50) for hsv-1, -2, and hcmv were 0.23, 0.04 and 0.40 micrograms/ml, respectively. the ec50 value of carbocyclic oxt-g against hsv-2 was significantly lower than those of acyclovir, gan ...19892559911
the effect of cimetidine on survival of mice infected with herpes simplex virus type 2, murine encephalomyelitis virus and vesicular stomatitis virus infections.the effect of cimetidine on survival was investigated in mice infected with herpes simplex virus type 2 (hsv-2), murine encephalomyelitis virus (gd-vii), and vesicular stomatitis virus (vsv). balb/c mice, 5 weeks of age, were injected intraperitoneally (i.p.) with 5.5 x 10(5) plaque-forming units (pfu) of virus/0.5 ml, and cimetidine (1 mg/0.5 ml) was administered simultaneously. the survival rates of 80% and 85% in the cimetidine groups were significantly greater than the 10% and 23% for the co ...19892561403
[epidemiological evaluations of human immunodeficiency virus, herpes simplex virus type 1 and 2 and cytomegalovirus infections in drug addicts].eighty-eight drug addicts from the "ban center" in torre annunziata (naples) and 88 normal subjects pair-matched for age and sex were tested for igg to human immunodeficiency virus (hiv), herpes simplex virus (hsv) type 1 and 2 and cytomegalovirus (cmv). a high prevalence of subjects with antibodies to hsv-1 and cmv (80.7% and 65.9%) were recorded in the control group testifying to the high level of these infections in campania. prevalences were higher in drug addicts, and drug abuse was identif ...19892562004
[characteristics and role of the gluteal herpes in a female population].based on the fact that the gluteal herpes may constitute the clinical expression of the reactivation of a herpes simplex virus latent at the sacral lymph node, we investigated a group of women who were carriers of gluteal herpetic infection, the characteristics of the infection, the virus type principally associated to it, and its possible relation with the genital herpes. forty one women with gluteal herpes verified by virologic laboratory were studied. 75.7% of these women had had in addition ...19892562294
[evaluation of 2 commercial monoclonal antibody kits for the identification of herpes simplex virus].two commercial monoclonal antibody kits (virgo and mikrotrak for the identification of herpes simplex virus (hsv) have been assessed. in 65 positive hsv cultures from clinical samples, 25 strains (38%) were identified as hsv1 with both kits. the virgo kit identified 37 strains (57%) as hsv2, and did not identify the remaining 3 (5%). with the microtrak kit, 34 strains (52%) corresponded to type 2, 2 (3%) strains remained nonidentified, and in the remaining 4 isolates (6%) both viral types were i ...19892562319
[maternal genital herpes: virus isolation during the third trimester of pregnancy and at delivery].we followed 42 pregnant women at high risk for genital herpes infection. viral isolation was attempted weekly from the 34th week of pregnancy. one or more episodes of herpes infection developed in 74% of women. the virus was isolated in 28% after 34 weeks and in 22% after 37 weeks. a prophylactic cesarean section was performed in the latter group; only one of these women had a positive viral isolation at delivery. the hsv-2 of virus was found in 83% of cases and hsv-1 in 17%.19892562415
hsv hepatitis in the mouse: a light and electron microscopic study with immunohistology and in situ order to characterize better the morphology and immune response in acute necrotizing hsv infection, murine hsv hepatitis was examined. balb/c mice were inoculated intraperitoneally with 10(6) plaque-forming units (pfu) of hsv-1 (lenette) and hsv-2 (d316). in both groups half the animals were pretreated with silica particles to block macrophage function. up to 6 days after infection four mice from each group were sacrificed at daily intervals and the livers were examined by light and electron ...19892567083
some immunological characteristics of subjects suffering from frequent herpes simplex virus recrudescences.some parameters of specific and non-specific immunity were tested in a group of 44 subjects suffering from frequent herpes simplex type 1 (hsv-1) or herpes simplex type 2 (hsv-2) recrudescences. the tests performed included determinations of (i) hsv complement-independent and complement-dependent neutralizing antibodies, (ii) antibodies to glycoprotein c of hsv-1 and glycoprotein g of hsv-2, (iii) antibodies to viral capsid and early antigens of epstein-barr virus, (iv) antibodies to tetanus tox ...19892576584
lack of antiviral activity of ketoconazole alone or in combination with the acyclic nucleoside ganciclovir against a herpes virus type 2 infection in mice.ketoconazole and ganciclovir were tested for antiviral activity, each alone and in combination, against a herpes simplex virus type 2 (hsv-2) systemic infection in swiss-webster mice. when given once daily for 5 days starting 24 hr after infection, the ed50 for ketoconazole either alone or in combination was greater than 60 mg/kg; for ganciclovir, the ed50 was 7.1 mg/kg alone and 10.8 mg/kg in combination. thus, ketoconazole did not potentiate or antagonize the antiviral activity of an acyclic n ...19892576599
studies on the herpes simplex virus alkaline nuclease: detection of type-common and type-specific epitopes on the enzyme.five monoclonal antibodies to the alkaline nuclease of herpes simplex virus (hsv) types 1 and 2 have been used in immunoperoxidase tests to demonstrate the nuclear localization of the enzyme within hsv-1- and hsv-2-infected cells and to purify the enzyme from cells infected with either virus by immunoadsorbant chromatography. affinity chromatography with a 32p-labelled extract of hsv-2-infected cells has enabled us to demonstrate that the nuclease eluting from the immunoadsorbant is a phosphopro ...19852578550
dna-binding protein associated with herpes simplex virus dna polymerase.purified preparations of herpes simplex virus type 2 dna polymerase made by many different laboratories always contain at least two polypeptides. the major one, of about 150,000 molecular weight, has been associated with the polymerase activity. the second protein, of about 54,000 molecular weight, which we previously designated icsp 34, 35, has now been purified. the purified protein has been used to prepare antisera (both polyclonal rabbit serum and monoclonal antibodies). these reagents have ...19852578573
human cytotoxic t cell clones directed against herpes simplex virus-infected cells. iii. analysis of viral glycoproteins recognized by ctl clones by using recombinant herpes simplex viruses.human cytotoxic t cell (ctl) clones specific for herpes simplex virus (hsv) type 1- and type 2-infected cells were generated and were analyzed with regard to the viral glycoproteins they recognize on autologous hsv-infected cells. by use of target cells infected with wild-type hsv strains, a gc deletion mutant of hsv-1, and hsv-1 x hsv-2 intertypic recombinants, some hsv-1-specific ctl clones were found to be directed against l region-encoded ga/b-1, and others against s region-encoded glycoprot ...19852579149
frequency of herpes simplex virus-specific helper t lymphocyte precursors in the lymph node cells of infected mice.we have established a limited dilution assay to estimate the frequency of herpes simplex virus type 1 (hsv)-specific, interleukin 2 (il 2)-producing helper t lymphocyte precursors (htl-p). the estimated frequency of such cells in suspensions of local lymph node (ln) cells 5 days after in vivo virus infection was 1:2470 to 1:5800. frequencies of htl-p in cells from uninfected mice were below levels of detection of our system and were judged to be below 1:100,000. removal of lyt-2+ cells from resp ...19852579150
antibody response to type-common and type-unique epitopes of herpes simplex virus polypeptides.herpes simplex virus type 1 (hsv1) and type 2 (hsv2) polypeptides with type-common and type-unique epitopes were identified using cross-adsorbed hyperimmune rabbit sera and western blotting techniques. twelve hsv1 and fourteen hsv2 polypeptides with type-specific epitopes were identified. cross-adsorbed human sera reacted to a subset of the type-specific epitopes defined by rabbit sera. human sera with both hsv1- and hsv2-specific antibodies were identified by their reaction to hsv1-type-specifi ...19852580054
rapid detection of cytomegalovirus in cell culture by indirect immunoperoxidase staining with monoclonal antibody to an early nuclear antigen.a method for the rapid detection of cytomegalovirus (cmv) in mrc-5 cells 48 h after inoculation with clinical specimens was developed. a commercially available monoclonal antibody to a cmv early nuclear antigen was used in an indirect immunoperoxidase (ipa) staining procedure performed directly on acetone-fixed cell monolayers in standard tubes (16 by 125 mm). of 190 clinical specimens tested, 30 specimens produced cmv cytopathic effect in tissue culture (tc-cpe) within 14 days after inoculation ...19852581991
herpes simplex virus type 2 transcripts in trigeminal ganglia during acute and latent infection in mice were inoculated intracerebrally with herpes simplex virus type 2 (hsv-2), and perfused at days 5, 8, 81, 108, and 130 after infection. trigeminal ganglia and roots were dissected out and embedded in paraffin. four 35s-labelled dna probes that contained immediate-early (icp-0, icp-4), delayed-early (thymidine kinase; tk), or late (morphological transforming region; mtr) genes were prepared. in situ hybridization methods or an immunoperoxidase antigen method using hsv-2 antibody were ap ...19892592985
a self-inducing runaway-replication plasmid expression system utilizing the rop protein.a highly efficient prokaryotic expression system has been developed that produces proteins at levels exceeding 150 micrograms/ml of culture medium. the system consists of a temperature-sensitive-copy-number plasmid that carries the rop gene and promoter downstream from the trp promoter. any sequence cloned into the pvuii site of the rop gene alters rop protein activity and causes lethal runaway plasmid dna replication. this plasmid replication can be suppressed in trans by complementation with a ...19892670683
characterization of a continuous smooth muscle cell line derived from rabbit aorta.a spontaneously arising continuous cell line (rb-1) derived from collagenase-elastase digested rabbit aorta has been propagated in vitro for over 100 passages. during this period, the rb-1 cells remained spindle-shaped and formed regularly oriented parallel bundles. after passage 50, rb-1 cells were found to be serum-independent in their growth and reached higher saturation density than rabbit aorta smooth muscle cells. alpha-actin and desmin filaments were detected by immunostaining in rb-1 cel ...19892681130
[acyclovir and pregnancy: current aspects].acyclovir (acv), an antiviral nucleoside analog, is active against herpes simplex viruses (hsv1, hsv2) and varicella virus (vzv). these viruses seems to be prejudicial to the pregnant woman and to the fetus. yet, acv is not recommended for use in pregnancy. however in certain cases, this drug has been used. we review in this paper, the pharmacokinetics and transplacental passage of acv, indications, and whether the benefits of the administration of acv in pregnancy outweigh the theoretical risks ...19892681370
a novel form of intrauterine infection by herpes simplex virus type 2: its association with spontaneous abortion, latent neonatal infection, and adult inflammatory diseases. 19892685911
[malpighian epithelia infected by dna viruses and langerhans cells].malpighian epithelia exhibit an immune surveillance through langerhans cells (lc) against the dna viruses, which induce various epithelial lesions, mainly on the skin, conjunctiva, mouth, uterine cervix. these viral infections are usual during childhood. the most common dna viruses are: adenoviruses (more than 40 types), human papillomaviruses (hpv, more than 50 types) and herpes viruses (herpes simplex type 1 and 2, varicella and zooster, epstein-barr virus). different clinical aspects are obse ...19892694082
[is mollaret's meningitis caused by herpes simplex virus type 2?].mollaret's meningitis is characterised by recurrent, brief episodes of serous meningitis with symptom-free intervals. although viral etiology has been suspected, no pathogenic agent has hitherto been found. using the immunoblot technique, we found igg antibodies of intrathecal origin against the hsv-2-specific protein, gg2, in a patient with mollaret's meningitis. since being put on prophylactic acyclovir treatment twelve months ago, the patient has had no further episodes of meningitis. because ...19892709930
5-(2-bromoethyl)-2'-deoxyuridine: a selective inhibitor of herpes simplex viruses in vitro.a new pyrimidine analog, 5-(2-bromoethyl)-2'-deoxyuridine (beudr), was tested in vitro for antiviral activity on herpes simplex virus types 1 and 2. as reference compounds, acg, bvudr and paa were used. compared to acg and bvudr, beudr resulted less potent on both hsv-1 and hsv-2. however, a 50% inhibition of the multiplication of uninfected cells could be obtained only at very high beudr concentration (id50 = 8500 microm). this makes beudr the least toxic analog known and gives it a selective i ...19892713989
herpetic stromal keratitis in congenital dysgammaglobulinemia. case report.the clinical picture and laboratory findings of a case of secondary herpes simplex virus type 2 in a patient with congenital dysgammaglobulinemia who was followed for 26 months is described. local combined therapy of acyclovir and decadron (dexamethasone) 0.1% was given for management for 14 months. the clinical and immunopathologic findings of our patient match the results described by other investigators in experimental animals.19892714093
the inhibitory effect of gossypol on human sperm motility: relationship with time, temperature and concentration.the inhibitory effect of gossypol acetic acid on human sperm motility was studied with a transmembrane migration method. gossypol decreased sperm motility after it had been incubated with semen for more than 15 min. however, when sperm motility was evaluated immediately after semen had been mixed with gossypol, no inhibitory effect could be found. we consider that the sperm immobilizing potency of gossypol is much less than our previously studied sperm immobilizing agents. it is unlikely that go ...19892714811
efficacy of phosphonylmethoxyalkyl derivatives of adenine in experimental herpes simplex virus and vaccinia virus infections in vivo.the phosphonylmethoxyalkyl derivatives (s)-9-(3-hydroxy-2-phosphonylmethoxypropyl)adenine [(s)-hpmpa], 9-(2-phosphonylmethoxyethyl)adenine (pmea), and 9-(2-phosphonylmethoxyethyl)-2,6-diaminopurine (pmedap) were evaluated for their in vivo efficacies in several animal model infections, i.e., mice infected intravenously with vaccinia virus and mice infected intracutaneously, intraperitoneally, or intracerebrally with herpes simplex virus type 1 (hsv-1) or type 2 (hsv-2) or thymidine kinase-defici ...19892719463
synthesis and antiviral activity of 5'-o-(substituted) sulfamoyl pyrimidine nucleosides.5'-o-[n-(aminoacyl or isobutyryl)sulfamoyl]uridines 4a-e, 5a-e and 5'-o-[n-(isopropyl)sulfamoyl]cytidines 7-9 have been synthesized and tested against herpes simplex virus type 2. condensation of 2',3'-o-isopropylidene-5'-o-sulfamoyluridine with the n-hydroxysuccinimide esters of boc-gly, boc-l-ala, boc-d-ala and boc-l-phe, gave 4a-d which, on deprotection under acidic conditions, provided 5a-d. a similar condensation of 2',3'-di-o-acetyl-5'-o-sulfamoyluridine with the n-hydroxysuccinimide ester ...19892730285
axonal degeneration and regeneration in sensory roots in a genital herpes a mouse model of genital herpes simplex virus type 2 (hsv-2) infection, roots of the lower spinal cord were examined 5 days to 6 months after inoculation. using immunoperoxidase methods on paraffin sections, viral antigen was found in sensory ganglia, their proximal roots and distal nerves on days 5 and 6 after infection. in epon sections, most mice had focal sensory root abnormalities in lower thoracic, lumbar or sacral levels. at days 7 and 10, lesions showed chiefly nerve fiber degeneratio ...19892750478
two cases of necrotizing myelopathy associated with malignancy caused by herpes simplex virus type 2.two cases of necrotizing myelopathy were autopsied; one was complicated with lung carcinoma and the other with chronic type adult t cell leukemia (atl leukemia). to our knowledge, they were the first cases of their type in japan. in both cases, necrosis of the spinal cord was observed in the gray and white matter along most of its extent. marked changes were found in the lumbar segment. the patients were not treated with intravenous cancer chemotherapy or irradiation. immunohistochemical and ele ...19892763797
antibody responses and protection in mice immunized with herpes simplex virus type 1 antigen immune-stimulating complex preparations.the formation of immune-stimulating complexes (iscoms) obtained by mixing the glycoside quil a with an antigen preparation derived from herpes simplex virus type 1 (hsv-1)-infected cell cultures using a zwitterionic detergent is described. the hsv-1 antigen preparation incorporated into iscoms elicited significantly greater antibody responses in mice than the same preparation administered together with aluminium hydroxide gel, and provided complete protection against hsv-1 or hsv-2 lethal, syste ...19892769234
t lymphocytes in genital lymph nodes protect mice from intravaginal infection with herpes simplex virus type 2.herpes simplex virus type 2 (hsv-2) is a human venereal pathogen that causes lethal neurological illness after intravaginal inoculation into balb/cj mice. intravaginal vaccination of mice with an attenuated strain of hsv-2 rapidly induces immunity to a lethal intravaginal challenge with wild-type hsv-2. this resistance is transferrable to syngeneic mice with genital lymph node (gln) cells but not with cells from other lymphoid sources. here we demonstrate that minimal numbers of hsv-2-stimulated ...19892783720
broad-spectrum in vivo antiviral activity of 7-thia-8-oxoguanosine, a novel immunopotentiating agent.a novel immunopotentiating agent, 5-amino-3-beta-d-ribofuranosylthiazolo [4,5-d]pyrimidine-2,7(3h,6h)-dione (7-thia-8-oxoguanosine), lacks virus-inhibitory properties in vitro but induces interferon and potentiates immune functions, such as natural killer cell activity. it was evaluated in rodent models to determine the spectrum of antiviral activity and effective treatment regimens. at 50 to 200 mg/kg given as single or divided intraperitoneal (i.p.) doses 1 day before virus inoculation, signif ...19892817849
evaluation of a monoclonal antibody typing system for herpes simplex virus.this study compared conventional viral isolation (vi) in cell cultures with a commercial product--virgo antigen detection system--for the identification and typing of herpes simplex virus (hsv). the system is designed to identify and type hsv from direct lesion smears (ds) and from cell culture smears (cs) infected with a swab from the patient's lesion by means of an indirect fluorescence assay (ifa) using hsv type-common and hsv-2 type-specific monoclonal antibodies. a total of 71 coded specime ...19872819800
intertypic recombinants of herpes simplex virus types 1 and 2 infected-cell polypeptide 4.the wild-type icp4s (infected-cell polypeptide 4) encoded by herpes simplex virus types 1 and 2 (hsv-1 and hsv-2) are functionally interchangeable. in order to test the functional interchangeability of their intramolecular domains, a series of intertypic icp4 genes was constructed and characterized to determine if any of the encoded chimeric proteins were functionally impaired. we generated the recombinants in escherichia coli using cloned icp4 genes and the lambda recombination vectors develope ...19872820127
occupational risk of hiv, hbv and hsv-2 infections in health care personnel caring for aids patients.we have prospectively followed for 9-12 months, 246 female health care workers (hcws): 102 with high exposure (he), 43 with low exposure (le), and 101 with no exposure (ne) to aids (acquired immunodeficiency syndrome) patients. no hcws have clinical, serologic, or immunologic evidence of hiv (human immunodeficiency virus) infection. no hcws in the he group seroconverted to cytomegalovirus (cmv). one hcw in the he group seroconverted to hepatitis b virus (hbv), another hcw in the he group serocon ...19872820252
limiting dilution analysis of specific in vitro anti-herpes simplex virus antibody production by human enzyme linked immunosorbent assay (elisa) system to study specific anti-herpes simplex virus (hsv) antibody production in vitro by human peripheral blood mononuclear cells (pbmc) has been developed. hsv specific antibody production was detected in culture supernatants of pbmc from hsv seropositive healthy individuals by stimulating with optimal concentrations of hsv antigen without mitogens. to investigate the specificity of the resulting antibody, a limiting dilution analysis was performed a ...19872820632
improved sensitivity of restriction endonuclease analysis of herpes simplex virus type 2 dna with polyacrylamide gradient gel electrophoresis.polyacrylamide gradient gel electrophoresis was used to resolve fragments of herpes simplex virus type 2 (hsv-2) dna, produced by the restriction endonucleases alu i, bam hi, pst i, and sma i, which cleave the hsv-2 dna into more than 30 fragments each. hsv-2 strains isolated from different individual patients could be easily distinguished from each other by the endonucleases bam hi and sma i. successive virus isolates from a single person, analyzed using alu i and sma i, showed variability of f ...19872821049
immunity to herpes simplex virus type 2. suppression of virus-induced immune responses in ultraviolet b-irradiated mice.ultraviolet b irradiation (280 to 320 nm) of mice at the site of intradermal infection with herpes simplex virus type 2 increased the severity of the herpes simplex virus type 2 disease and decreased delayed-type hypersensitivity (dth) responses to viral antigen. decrease in dth resulted from the induction of suppressor t cells, as evidenced by the ability of spleen cells from uv-irradiated mice to inhibit dth and proliferative responses after adoptive transfer. lymph node cells from uv-irradiat ...19872821119
replication characteristics and core size of intranuclear herpes simplex virus (hsv-1) in genital skin lesions: electronmicroscopy studies of a biopsy from a female patient.herpes simplex virus (hsv) type 1 genital infection, leading to ulcerating lesions in a female patient, was studied by electronmicroscopy. infection had probably been recent, through oro-genital contact with a cold sore on the husband's lip. cell-culture typing and serological tests indicated that the patient currently had an hsv-1 secondary infection. aspects studied in a skin biopsy from an ulcerating labium majus were epidermal cell types infected, stages in virus genesis, virus core diameter ...19872821262
clinical isolate of herpes simplex virus type 2 that induces a thymidine kinase with altered substrate vitro and in vivo studies were done on a herpes simplex virus type 2 strain recovered from a patient on acyclovir (acv) which was acv resistant but expressed thymidine (dthd) kinase (ec activity. plaque-purified clones derived from the original clinical sample were heterogeneous with respect to plaque size and drug susceptibility. the heterogeneity of this viral mixture was also evident from varied 125i-labeled 5-iodo-2'-deoxycytidine autoradiographic patterns and from varied expres ...19872821890
in vitro and in vivo antiviral activity of 2'-fluorinated arabinosides of 5-(2-haloalkyl)uracil.5-(2-fluoroethyl)-2'-deoxyuridine (fedu), its 2'-fluoroarabinofuranosyl analog (fefau) and the 2'-fluoroarabinofuranosyl analog (cefau) of the potent anti-herpesvirus compound 5-(2-chloroethyl)-2'-deoxyuridine (cedu) were evaluated for activity against herpes simplex virus type 1 (hsv-1) and hsv-2 in vitro and in vivo. fedu, fefau and cefau proved to be potent and selective anti-herpesvirus agents in vitro. their potency is evident from their low minimum inhibitory concentrations for hsv-1 and h ...19872821896
activation of latent herpes simplex virus type 2 infection in vitro requires a (e)-5-(2-bromovinyl)-2'-deoxyuridine-sensitive gene function.previous studies have shown that herpes simplex virus (hsv) type 2 (hsv-2) can be maintained in a latent state in a limited number of cells by elevating the incubation temperature after treatment of hsv-infected human fetus lung fibroblast cells with metabolic inhibitors. superinfection with human cytomegalovirus (hcmv) of latently infected cells maintained at the elevated temperature reactivated latent virus. in addition, superinfection with temperature-sensitive mutants indicated that reactiva ...19872822602
specific phosphorylation of 5-ethyl-2'-deoxyuridine by herpes simplex virus-infected cells and incorporation into viral dna.5-ethyl-2'-deoxyuridine (edu) is a potent and selective inhibitor of the replication of herpes simplex virus type 1 (hsv-1) and 2 (hsv-2), which is currently being pursued for the topical treatment of hsv-1 and hsv-2 infections in humans. using [4-14c]edu as the radiolabeled analogue of edu, it was ascertained that, at antivirally active doses, edu is phosphorylated to a much greater extent by hsv-infected vero cells than by mock-infected cells. within the hsv-1-infected cells, edu was incorpora ...19872822705
a sensitive enzyme-linked immunosorbent assay for the detection of herpes simplex virus antigens.a sandwich elisa for the detection of herpes simplex virus (hsv) antigens was developed using sheep anti-hsv f(ab')2 fragments for capture and an indirect antibody system for detection. current detection limits are 0.5 ng protein for hsv1 and 1.5 ng protein for hsv2. this compares to a single hsv1-infected vero-cell in a background of 10(6) non-infected cells or 10 plaque forming units (pfu) of hsv1 in culture supernatants as determined in separate experiments. limiting dilution experiments show ...19872822751
acetylenic nucleosides. 4. 1-beta-d-arabinofuranosyl-5-ethynylcytosine. improved synthesis and evaluation of biochemical and antiviral properties.5-ethynyl-1-beta-d-arabinofuranosylcytosine (eac) was prepared from 1-(2,3,5-tri-o-acetyl-beta-d-arabinofuranosyl)cytosine by iodination followed by coupling with (trimethylsilyl)acetylene and deblocking. at 50 microm, eac was found to inhibit the in vitro replication of herpes simplex virus type 1 and type 2 by greater than 99%. eac also showed activity against a strain of hsv-1 resistant to (e)-5-(2-bromovinyl)-2'-deoxyuridine which has an alteration of the virus-induced thymidine kinase (tk). ...19872822932
evidence that neomycin inhibits binding of herpes simplex virus type 1 to the cellular receptor.the effect of neomycin, a phosphoinositide-binding aminoglycoside, on herpes simplex virus type 1 (hsv-1) infection of bhk cells was studied. we showed earlier that it specifically inhibits hsv-1 production but not hsv-2 production (langeland et al., biochem biophys. res. commun. 141:198-203, 1986). we now show that neomycin had no effect on cellular protein synthesis, as judged by the appearance of 35s-labeled polypeptides separated by polyacrylamide gel electrophoresis. virus-induced polypepti ...19872822948
thymidylate synthase is the principal target enzyme for the cytostatic activity of (e)-5-(2-bromovinyl)-2'-deoxyuridine against murine mammary carcinoma (fm3a) cells transformed with the herpes simplex virus type 1 or type 2 thymidine kinase gene.murine mammary carcinoma fm3a cells, deficient in cytosol thymidine (dthd) kinase (tk) activity and transformed by the herpes simplex virus type 1 (hsv-1) or type 2 (hsv-2) tk gene (designated fm3a tk-/hsv-1 tk+ and fm3a tk-/hsv-2 tk+, respectively) proved extremely sensitive to the cytostatic action of the potent antiherpetic drugs (e)-5-(2-bromovinyl)-2'-deoxyuridine (bvdu) and (e)-5-(2-iodovinyl)-2'-deoxyuridine (ivdu). the fact that fm3a tk-/hsv-2 tk+ cells were 5-fold more sensitive to the ...19872823092
effects on infants of a first episode of genital herpes during pregnancy.although genital herpes simplex virus (hsv) infections occurring during pregnancy are known to be associated with neonatal and maternal complications, their frequency and contributing risk factors are not well understood. we prospectively followed 29 patients who acquired genital herpes during pregnancy, to evaluate the perinatal effects of the infection. the patients were classified on the basis of clinical or serologic criteria. fifteen patients had a primary first episode of genital hsv type ...19872823137
[(e)-5-(2-bromovinyl)-2'-desoxyuridine--a new nucleoside analog with selective inhibitory action against herpesviruses. studies in cell culture and animal experiments].(e)-5-(2-bromovinyl)-2'-deoxyuridine (1; brvudr) inhibits the replication of herpes simplex virus type 1 (hsv-1) and of varicella-zoster virus (vzv) in vitro at concentrations of 0.01 to 0.23 mumol/l, whereas herpes simplex virus type 2 (hsv-2) is influenced only at 5.5 to 27 mumol/l. in comparison to some classical and newly developed antiherpetics, i. e. 5-iodo-2'-desoxyuridine (2; idoxuridine, idu), 9-beta-d-arabinofuranosyladenine (4; vidarabine ara-a), 9-(2-hydroxyethoxymethyl) guanine (5; ...19872823299
study of ribonucleotide reductase in cells infected with six clinical isolates of herpes simplex virus type 2 (hsv-2) with mutations in its larger subunit.herpes simplex virus type 2 (hsv-2) induces a novel ribonucleotide reductase (rr) composed of two subunits (140 and 38 kda) in infected cells. other investigators have developed a monoclonal antibody, a6, against the 140-kda subunit of rr and have found, in about 1% of the cases, an inability to detect this protein in cells infected with clinical isolates of hsv-2. we therefore investigated whether in such cases the clinical isolates were capable of inducing viral rr activity and whether the lac ...19872823465
critical determinants of antiherpes efficacy of buciclovir and related acyclic guanosine analogs.buciclovir is an example of an antiherpes, acyclic guanosine analog activated by the viral thymidine kinase and inhibiting viral dna synthesis in infected cells. an investigation of closely related buciclovir-analogs with similar antiherpes activities in cell cultures and similar, or identical, modes of action but with disparate effects in vivo, revealed the following critical determinants of antiherpes efficacy. (1) the accumulation of guanosine analog-triphosphates in infected cells, which is ...19872823702
redistribution of nuclear ribonucleoprotein antigens during herpes simplex virus infection.infection of human epidermoid carcinoma no. 2 cells with herpes simplex virus type 1 (hsv-1) leads to a reorganization of antigens associated with both the small and heterogeneous nuclear ribonucleoprotein complexes (snrnp and hnrnp). the hnrnp core protein antigens remain associated with the host chromatin, which appears to collapse into internal aggregates and along the nuclear envelope. more striking is the formation of prominent clusters of snrnp antigens (both general and u1 snrnp specific) ...19872824525
herpes simplex virus glycoproteins gc-1 and gc-2 bind to the third component of complement and provide protection against complement-mediated neutralization of viral infectivity.cells infected with herpes simplex virus type 1 (hsv-1) form rosettes with c3b-coated erythrocytes, whereas cells infected with herpes simplex virus type 2 (hsv-2) or other herpes viruses do not. it was reported that glycoprotein c of hsv-1 (gc-1) mediates the binding of c3b-coated erythrocytes to infected cells and has regulatory (decay-accelerating) activity for the alternative pathway c3 convertase of human complement. we show here that solubilized gc-1 binds to ic3-sepharose affinity columns ...19872824652
regulation of glycoprotein d synthesis: does alpha 4, the major regulatory protein of herpes simplex virus 1, regulate late genes both positively and negatively?earlier studies have described the alpha 4/c113 baby hamster kidney cell line which constitutively expresses the alpha 4 protein, the major regulatory protein of herpes simplex virus 1 (hsv-1). introduction of the hsv-1 glycoprotein b (gb) gene, regulated as a gamma 1 gene, into these cells yielded a cell line which constitutively expressed both the alpha 4 and gamma 1 gb genes. the expression of the gb gene was dependent on the presence of functional alpha 4 protein. in this article we report t ...19882824843
entry of herpes simplex virus 1 in bj cells that constitutively express viral glycoprotein d is by endocytosis and results in degradation of the virus.the bj cell line which constitutively expresses herpes simplex virus 1 glycoprotein d is resistant to infection with herpes simplex viruses. analysis of clonal lines indicated that resistance to superinfecting virus correlates with the expression of glycoprotein d. resistance was not due to a failure of attachment to cells, since the superinfecting virus absorbed to the bj cells. electron microscopic studies showed that the virions are juxtaposed to coated pits and are then taken up into endocyt ...19882824844
a human cytomegalovirus function inhibits replication of herpes simplex virus.human embryonic lung (hel) cells infected with human cytomegalovirus (hcmv) restricted the replication of herpes simplex virus type 1 (hsv-1). a delay in hsv replication of 15 h as well as a consistent, almost 3 log inhibition of hsv replication in hcmv-infected cell cultures harvested 24 to 72 h after superinfection were observed compared with controls infected with hsv alone. treatment of hcmv-infected hel cells with cycloheximide (100 micrograms/ml) for 3 or 24 h, conditions known to result i ...19882824846
differential metabolism of (e)-5-(2-iodovinyl)-2'-deoxyuridine (ivdu) by equine herpesvirus type 1- and herpes simplex virus-infected cells.thymidine kinase (tk) enzymes encoded by herpes simplex viruses types 1 and 2 (hsv-1, hsv-2), and equine herpesvirus type 1 (ehv-1) catalyze the phosphorylation of thymidine (dthd) and (e)-5-(2-bromovinyl)-2'-deoxyuridine (bvdu). the replication of hsv-1 is sensitive to bvdu, but the replication of hsv-2 and ehv-1 is not. to investigate the differential sensitivity of the viruses to halogenated vinyldeoxyuridine drugs, the phosphorylation of 125i-labeled (e)-5-(2-iodovinyl)-2'-deoxyuridine (ivdu ...19872825591
suppression of humoral antibody formation against sheep red blood cells by infections with hsv-2 and the influence of mouse cytomegalovirus. brief report.hsv-2 infections suppress the antibody response to hsv-1 but do not impair the mouse cytomegalo virus (mcmv) antibody generating system. in contrast, the sheep red blood cell (srbc) igm response is impaired by preinfections with hsv-2 but not with hsv-1. from the time kinetics of this suppression it can be concluded that only a certain submechanism "spills over" to the srbc system. mcmv suppresses the antibody formation induced by hsv-1.19872825620
ribonucleotide reductase induced by varicella zoster virus. characterization, and potentiation of acyclovir by its enzyme that catalyzes the conversion of cdp to 2'-dcdp in the presence of dithiothreitol (dtt) was detected in ammonium sulfate fractionated-extracts of varicella zoster virus (vzv)-infected cells. this ribonucleotide reductase was antigenically distinguishable from the isofunctional eucaryotic enzyme as well as the ribonucleotide reductases induced by herpes simplex virus types 1 and 2 (hsv-1 and hsv-2). the vzv-induced enzyme was purified to the extent that most of the contaminating enzymes ...19872825724
reinfection of guinea pigs with herpes simplex virus type 2: failure to establish a second latent ganglionic infection.guinea pigs were infected with herpes simplex virus type 2 (hsv 2) and at various times thereafter reinfected at a different site with the same or a different strain of hsv 2. viruses were isolated from infected tissues either from the site of primary or secondary infection or from regional ganglia. viral isolates were characterised by dna fingerprinting using restriction endonucleases capable of differentiating between the virus strains used. the second infection resulted in a second site of pe ...19872825951
monoclonal antibody to hsv2 protein as an immunodiagnostic marker in cervical cancer.the present study was designed to evaluate the possible use of monoclonal antibodies (mabs) as diagnostic adjuncts to exfoliative cytology and tissue sections in intraepithelial (cin) and invasive cervical cancer. specimens were collected from 42 patients with various degrees of cin, 15 patients with invasive cancer and two patients with condylomatous changes only. mab h17, that recognizes a herpes simplex virus protein (icp) representing a component of the viral ribonucleotide reductase, staine ...19872826000
simultaneous presence of herpes simplex and human papilloma virus sequences in human genital tumors.herpes simplex virus (hsv) and human papillomavirus (hpv) sequences were analyzed in tumors of the female lower genital tract, by probing dna from 13 intraepithelial and 30 invasive neoplastic lesions with radiolabelled hpv-16 and hpv-18 dna as well as cloned fragments of hsv-2 dna. careful removal of stromal tissue from the pathological specimens allowed authentic tumor dna to be processed. normal genital tissue obtained from the patients and genital condylomata were included as internal contro ...19872826337
dna binding of a 38,000-dalton herpes-simplex-virus-2-specific protein.western transfer and immunoenzymatic staining with affinity-purified monospecific antiserum was used to detect a 38-kd protein that bound to native and denatured dna cellulose. this protein has previously been shown to be a delayed early herpes simplex virus type-2 specific protein.19872826362
herpes simplex virus glycoprotein treatment of recurrent genital herpes.we investigated the possibility that herpes simplex virus (hsv) glycoproteins might provide potential immunotherapy for recurrent hsv disease by using the guinea pig model of genital hsv infection. in two experiments, after recovery from initial genital herpes, 58 animals were randomized either to receive a glycoprotein vaccine or to serve as controls. both a mixture of hsv-2 glycoproteins derived from infected tissue cultures and a mixture of hsv-1 glycoproteins b and d, prepared by genetic eng ...19882826603
type-specific antibodies to herpes simplex virus type 2 (hsv-2) glycoprotein g in pregnant women, infants exposed to maternal hsv-2 infection at delivery, and infants with neonatal herpes.we used a murine monoclonal antibody to herpes simplex virus (hsv) type 2 (hsv-2) glycoprotein g (gg) to develop an enzyme immunoassay that detected hsv-2 type-specific antibodies in human sera. antibodies to hsv-2 gg were detected in 98 (96%) of 102 sera from pregnant women with culture-proved hsv-2 infection. sixty-five percent of the women had serological evidence of past hsv-2 infection by the rawls index, based on titers of neutralizing antibody to hsv type 1 and hsv-2. thirty (88%) of 34 i ...19882826604
generation of a herpes simplex virus type 2 variant devoid of xbai sites: removal of the 0.91 map coordinate site results in impaired synthesis of glycoprotein g-2.herpes simplex virus (hsv) type 2 (strain hg52) has four xbai sites at map coordinates (m.c.) 0.45, 0.7, 0.91 and 0.94, i.e. two in the unique long and two in the unique short regions of the genome. previously, we had isolated a genome containing only the 0.45 and 0.94 xbai sites. here we report the isolation of a mutant (jh2611) in which all four xbai sites have been removed using an enrichment selection procedure, without any loss of viability. removal of each site has been shown to be due to ...19882826660
herpes simplex virus infection of the hand. a profile of 79 cases.seventy-nine cases of herpes simplex (hsv) infection of the hand were documented by viral culture over a 40-month period. a marked bimodal age distribution was found with 32 cases occurring in adults aged 21 to 30 years and 16 occurring in children less than 10 years old. the infection most commonly occurred on the fingers (69 percent) and thumb (21 percent). all 20 herpes simplex type 2 (hsv-2) isolates came from patients over 20 years old. all of the 13 specimens that were typed after isolatio ...19882827469
glycosylation pattern of herpes simplex virus type 2 glycoprotein g from precursor species to the mature form.the changes in the apparent molecular weight of herpes simplex virus type 2 glycoprotein g (gg2) were studied by using different [3h] mannose labeling time intervals. various size classes of precursors, probably derived from proteolytic cleavage of the translational product, were identified. our experiments provide evidence that only the 74 kd species is the real precursor of the mature 120 kd gg2. the increase in size is due for the most part to the assembly of o-linked oligosaccharides and to ...19872827609
nuclear accumulation of a heat-shock 70-like protein during herpes simplex virus replication.a monoclonal antibody defines an antigen, p68, related to hsp70, which is located in nuclei of uninfected exponential cells. nuclear p68 is released by dnase but not rnase treatment suggesting an association with dna. lytic productive infection of confluent quiescent bhk 21 cells with herpes simplex virus type-2 causes p68 to accumulate in nuclei. the effect is specific for hsv-2, and does not occur in hsv-1 infected cells. maximum nuclear accumulation of p68 requires virus dna synthesis althoug ...19872827804
use of densitometric analysis for interpreting hsv serologies based on western blot.western blot assays have been described for detecting antibodies to herpes simplex virus (hsv). a predominance of antibody binding to either the hsv-1 or the hsv-2-containing blot has been reported to indicate infection with hsv-1 or hsv-2, respectively. by densitometry, differential binding of total antibody on hsv-1 versus hsv-2 strips can be expressed as a ratio. to determine the clinical correlation of these ratios, sera from 81 patients with culture-proven oral or genital herpes were tested ...19872828399
typing of herpes simplex virus types 1 and 2 by immunoblotting analysis using polyclonal antisera to herpes simplex virus glycoproteins.herpes simplex virus (hsv) isolates were differentiated by immunoblotting analysis using a mixture of polyclonal antisera directed against hsv type 1 (hsv-1) and hsv type 2 (hsv-2) glycoprotein fractions (gb/gc of hsv-1 and gc/ge/84-kda protein of hsv-2), since the mixed antisera recognized viral polypeptides with different molecular weights in hsv-1- and hsv-2-infected cells. results of typing by immunoblotting analysis were consistent with those obtained by restriction endonuclease analysis of ...19872828400
herpes simplex virus type 2 establishes latency in the mouse footpad.balb/c mice were infected with herpes simplex virus type 2 by inoculation into the footpad. three months or more after infection, and in the total absence of any evidence of illness, latent virus could be reactivated from both ganglia and footpad tissue but only after explantation and culturing for a week or more. virus recovery from the footpad could not be prevented by treatment of the infected mice with acycloguanosine for up to 6 months in their drinking water at 40 mg/kg, nor by femoral and ...19882828516
herpes simplex virus type 2 latency in the footpad of mice: effect of acycloguanosine on the recovery of virus.herpes simplex virus type 2 has been reactivated from the latent state in the footpad and dorsal root ganglia of acycloguanosine-treated balb/c mice. virus was also recovered from the footpad tissue but not from the ganglia of denervated, latently infected mice. treatment in vitro of explanted footpad cultures with acycloguanosine or phosphonoacetic acid did not affect the rate of virus reactivation. in all the isolates examined the virus was found to be acycloguanosine-sensitive. recovery of vi ...19882828517
the effect of triterpenoid compounds on uninfected and herpes simplex virus-infected cells in culture. iii. ultrastructural study of virion maturation.electron microscope studies been made of mock-infected or herpes simplex virus (hsv) type 1 (strain 17)- or hsv-2(strain hg52)-infected flow 2002 cells grown in the absence or presence of various concentrations of cicloxolone sodium (ccx). fifty non-serial, thin sections of mock-infected or hsv-infected cells, which contained a portion of the nucleus, were examined by transmission electron microscopy. with increasing drug concentration, counts of capsid structures both in the nucleus and cytopla ...19882828518
synthesis and biological activities of 4-o-(difluoromethyl)-5-substituted-uracil nucleoside analogues.various 4-o-difluoromethyl analogues of 5-substituted uridine (urd), 2'-deoxyuridine (durd), and arabinofuranosyluracil (arau) nucleosides were prepared via a cf2-insertion reaction into 4-o-silylated nucleosides and evaluated for activity against herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) and cytotoxicity in human embryonic lung fibroblast (helf) cell cultures. the introduction of the 4-substituent led to a strong reduction of antiviral activity for durd but not for arau analogues. ...19882828623
herpes simplex virus virion host shutoff function.herpes simplex virus (hsv) virions contain one or more functions which mediate the shutoff of host protein synthesis and the degradation of host mrna. hsv type 1 (hsv-1) mutants deficient in the virion shutoff of host protein synthesis (vhs mutants) were isolated and were found to be defective in their ability to degrade host mrna. furthermore, it was found that viral mrnas in cells infected with the vhs 1 mutant have a significantly longer functional half-life than viral mrnas in wild-type viru ...19882828686
prior herpes simplex virus type 2 infection as a risk factor for hiv infection.antibodies to cytomegalovirus (cmv), epstein-barr virus (ebv), and herpes simplex viruses types 1 and 2 (hsv-1, hsv-2) in three sequential serum samples from 62 men who did and 61 men who did not develop human immunodeficiency virus (hiv) infection at the time of the final (third) serum specimen were studied. antibody titers to cmv, ebv, and hsv-1 did not significantly rise in or differ between men who did or did not get hiv infection. however, we found that 32 (68%) of 47 hiv seroconverters had ...19882828700
role of herpes simplex virus type-2 and human papilloma virus in penile cancers in detect the presence of herpes simplex virus type 2 (hsv-2) and human papilloma virus (hpv), common structural antigens in penile cancers, 20 squamous cell carcinomas were studied by immunohistochemical methods. all specimens were from singaporeans ranging in age from 44 to 69 years. none of the specimens contained hsv-2 and hpv common structural antigens. the reasons for the absence of hpv common antigen were discussed. the significance of the association of hpv with penile cancers is uncerta ...19872829697
nucleotide sequence changes in thymidine kinase gene of herpes simplex virus type 2 clones from an isolate of a patient treated with identify the nucleotide changes that occur in drug-induced thymidine kinase (tk) mutants of herpes simplex virus type 2 (hsv-2), we compared the nucleotide sequences of the tk genes of two mutant hsv-2 clones isolated from a patient who had been treated with acyclovir [9-(2-hydroxyethoxymethyl)guanine; acv] with the nucleotide sequence of the parental tk+ hsv-2(8703) strain isolated from the same patient. one of the mutants, tk-altered (tka) hsv-2(9637), was acv resistant but induced the inco ...19872829709
inactivation of herpes simplex virus types 1 and 2 by synthetic histidine peptides.synthetic homologous histidine peptides were found to directly and irreversibly inactivate herpes simplex virus types 1 and 2 (hsv-1 and hsv-2). the inactivation, which occurred within 1 min of virus exposure to the drug, was independent of temperature but dependent upon the ph and molecular size of the polypeptide. poly-l-histidine consisting of 24 residues (his-24), with a molecular weight (m.w.) of 3,310, inactivated greater than 99% of the virus present at ph 6.0 and 62% at ph 5.0. poly-l-hi ...19872829711
delta-9-tetrahydrocannabinol inhibits the splenocyte proliferative response to herpes simplex virus type 2.the present investigation was undertaken to determine the effect of in vivo delta-9-tetrahydrocannabinol (delta-9-thc) treatment on immune responsiveness to secondary exposure to herpes simplex virus type 2 (hsv2) antigens in vitro. a splenocyte proliferative assay, employing hsv2-infected mouse embryo fibroblasts as target cells, was used to measure immune responsiveness. administration of 50 mg/kg or 100 mg/kg delta-9-thc to b6c3f1 mice in concert with hsv2 infection resulted in suppression of ...19872830329
transactivation of human immunodeficiency virus by herpesviruses.we examined the interaction of human immunodeficiency virus (hiv) and herpes group viruses. for this purpose, a chimeric plasmid (pltr-cat) was constructed in which the long terminal repeat (ltr) sequences derived from a molecular clone of hiv were fused to a bacterial chloramphenicol acetyltransferase gene (cat). transient expression assays in transfected tissue culture cells were used to monitor the activity of the ltr. basal levels of cat activity were measured in hela and human lung fibrobla ...19872830574
[biological effects of coordinated compounds of transitional metals. 7. antiviral activity of 4-methyl-2-aminopyridine palladium chloride against herpes simplex virus type 1 and type 2 in vitro and in vivo].4-methyl-2-amino-pyridine-palladium chloride (map) showed an antiviral activity against herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) in a serum-free medium under in vitro conditions. the replication of these viruses on primary rabbit testes cells was completely suppressed by 10(-5) m/l map. in animal tests using abd2-mice the course of hsv-1 and hsv-2 encephalitis was not influenced by map indicated by mean survival time and lethality.19872830739
insertion and extension of acyclic, dideoxy, and ara nucleotides by herpesviridae, human alpha and human beta polymerases. a unique inhibition mechanism for 9-(1,3-dihydroxy-2-propoxymethyl)guanine triphosphate.the ability of human alpha and beta dna polymerases and herpes simplex virus type 2 (hsv-2) and human cytomegalovirus (hcmv) dna polymerases to insert and extend several nucleotide analogs has been investigated using a variation of sanger-coulson dna sequencing technology. the analogs included the triphosphates of two antiviral nucleosides with incomplete sugar rings: 9-(1,3-dihydroxy-2-propoxymethyl)guanine (dhpg) and 9-(2-hydroxyethoxymethyl)guanine (acyg or acyclovir), as well as dideoxy and ...19882831212
evidence that the gene for herpes simplex virus type 1 dna polymerase accounts for the capacity of an intertypic recombinant to spread from eye to central nervous system.hsv-1(17) replicates 100-fold more efficiently than hsv-2(186) within trigeminal ganglia following ocular infection. in order to identify the nucleotide sequences responsible for the differences in the capacity of the two hsv strains to grow within the peripheral nervous system, an intertypic recombinant was generated by infecting neuroblastoma cells with hsv-2(186) and a hsv strain possessing nucleotide sequences from hsv-1(17). the genome of the intertypic recombinant was composed entirely of ...19882831653
zwitterionic detergent solubilisation of hsv-1 surface antigens.a preparation was obtained from herpes simplex virus type 1 (hsv-1)-infected cells using a zwitterionic detergent, empigen bb. the preparation was partially-purified either by ultracentrifugation over a cusion of 20% sucrose or on a sucrose density gradient. partial characterisation of these materials by elisa, using both polyclonal and monoclonal antibodies showed them to contain at least four major hsv glycoproteins, gb, gc, gd and ge. comparison of empigen-extracted hsv-1 antigen preparations ...19882831853
genital virus infection and cervical neoplasia.the reported association between genital infection by herpes simplex virus (hsv) type 2 and cervical neoplasia has been investigated at a molecular level. tissue culture of over 50 cervical cancers resulted in the development of several cell lines but none of these expressed hsv information. the permanent cell line, cx106, which was generated was found to contain multiple genome copies of human papillomavirus (hpv) type 16. southern blot analysis of nine cervical cancers showed an authentic 2.8 ...19882831934
trifluorothymidine: potential non-invasive diagnosis of herpes simplex infection using 19f nuclear magnetic resonance in a murine hepatitis model.trifluorothymidine (tft) is known to be concentrated in herpes simplex virus (hsv) infected cells in vitro in the form of phosphorylated derivatives. we studied a murine hepatitis model of hsv infection to determine whether this in vitro observation would also be demonstrable in vivo. following i.v. injection of 100 or 160 mg/kg tft, tft was found in significantly higher concentrations in the livers of hsv-2 infected mice than in the livers of uninfected mice, mice infected with murine hepatitis ...19872832432
antiviral activity of chamaecyparis lawsoniana extract: study with herpes simplex virus type 2.ethanolic extract of the green part of chamaecyparis lawsoniana was tested for antiviral activity and toxicity in tissue culture. all experiments were carried out in confluent human embryo lung fibroblasts. treatment of fibroblast cells with extracts after viral inoculation was effective in inhibiting the replication of herpes simplex virus type 2 (hsv-2). the critical time for virus inhibition was 4 to 5 h after virus absorption. the antiviral activity was assayed employing the techniques of vi ...19882832710
use of routine viral cultures at delivery to identify neonates exposed to herpes simplex virus.we obtained specimens for viral culture from mothers, infants, or both at the time of 6904 deliveries, without regard to the mothers' history of genital herpes. herpes simplex virus (hsv) was recovered in cultured specimens from 14 of the 6904 deliveries (0.20 percent); all 14 mothers were asymptomatic. all viral isolates were herpes simplex virus type 2 (hsv-2). only 1 of the 14 women (7 percent) had a history of genital herpes, whereas 12 (86 percent) had serologic evidence of a previous infec ...19882832756
expression of the hsv-2 ribonucleotide reductase subunits in adenovirus vectors or stably transformed cells: restoration of enzymatic activity by reassociation of enzyme subunits in the absence of other hsv proteins.we have cloned the large subunit (rr1) of the hsv-2 ribonucleotide reductase into a helper-independent adenovirus 5 vector under control of the viral major late promoter. infection of 293 cells with the adred-1 recombinant virus resulted in the expression of the hsv-2 rr1 protein. we have also produced cells which constitutively express the small (rr2) subunit of the hsv-2 enzyme by transfecting 293 cells with a plasmid encoding this protein and the neo resistance marker (psv2neo-rr2). infection ...19882833019
enhanced in vitro reactivation of latent herpes simplex virus from neural and peripheral tissues with hexamethylenebisacetamide.we evaluated the effect of the demethylating agent hexamethylenebisacetamide on reactivation of latent herpes simplex virus type 2 (hsv-2) from guinea pig neural and extraneural tissues. four explant cultures from the dorsal root ganglia of 42 latently infected guinea pigs and vaginal and cervical explant cultures from 33 animals were divided so that half received 5 mm of hexamethylenebisacetamide supplemented media and half media alone. hsv-2 was recovered earlier and from a greater percentage ...19882833203
human papillomavirus, herpes simplex virus and cervical cancer incidence in greenland and denmark. a population-based cross-sectional greenland, the incidence of cervical cancer is 5.7 times higher than in denmark among women aged 20-39. from nuuk (greenland) and nykøbing falster (denmark) a sample of 800 women aged 20-39 years was drawn at random. a total of 586 and 661 women were investigated in greenland and denmark respectively. all women had a gynecological examination including a pap-smear and cervical scrape for hpv-analysis (filter in situ hybridization). a blood sample was taken for analysis of hsv type-specific an ...19882833450
the epidemiology of infection with the human herpesviruses in navajo children.investigations of serum antibody status to the five human herpesviruses--herpes simplex virus type 1, herpes simplex virus type 2, cytomegalovirus, epstein-barr virus, and varicella-zoster virus--were conducted on 197 navajo children, aged 1-15 years, at a reservation pediatric outpatient clinic in crownpoint, new mexico, from 1981-1984. to determine the severity of infection with the human herpesviruses, the authors reviewed lifetime medical records of 166 of the children for evidence of herpes ...19882833854
effect of progesterone on the replication of herpes simplex virus type 2 in vitro.this project involved an investigation into the effect of progesterone on the replication of herpes simplex virus type 2 in a vero-cell culture. the first part of the experiment relates to preincubation of the cells with progesterone in the duration of 48 hours. after innoculation, replication of the virus was continued in the presence of progesterone. samples for titration were taken 10, 21 and 32 hours after innoculation for the purpose of determining the single-cycle replication curve of herp ...19882834117
effect of progesterone and pregnancy on the replication of herpes simplex virus type 2 in vivo.this project involved an investigation into the effect of pregnancy and progesterone on genital herpes simplex virus (hsv) type 2 infection in mice, with the aim of establishing whether elevated progesterone level, due either to pregnancy or parenteral administration, makes the vaginal hsv infection all the heavier. the investigation was carried out on three groups of mice, representing three test situations in connection with vaginal hsv infection: the first group consisted of non-pregnant expe ...19882834118
efficacy of recombinant glycoprotein d subunit vaccines on the development of primary, recurrent, and latent genital infections with herpes simplex virus type 2 in guinea pigs.vaccination of guinea pigs with recombinant glycoprotein d (subunit vaccine) from either herpes simplex virus type 1 or type 2 (hsv-1, hsv-2), before intravaginal inoculation with hsv-2, markedly altered viral replication in the vaginal tract and reduced, or completely prevented, the development and spread of external genital lesions. vaccination with glycoprotein d from hsv-2 prevented the establishment of latent hsv-2 infections in dorsal root ganglia and reduced the frequency of recurrent epi ...19882834467
antibodies to simian vacuolating virus 40 in bladder cancer patients.the occurrence of antibodies to herpes simplex virus type 2 (hsv-2) and simian vacuolating virus 40 (sv40) (two presumptive oncogenic viruses) was investigated by the technique of enzyme-linked immunosorbent assay in 233 patients with bladder cancer (200 males and 33 females) and in 466 controls (400 males and 66 females). the age of both the bladder cancer patients and controls varied from 55 to 65 years. a statistically significant association between bladder cancer and antibodies to sv40 was ...19872834853
human papillomavirus and herpes simplex virus in vulvar squamous cell carcinoma in situ.human papillomavirus deoxyribonucleic acid were detected in tissue specimens from 38 to 46 patients (83%) with squamous cell carcinoma in situ of the vulva. herpes simplex virus type 2--related antigen icsp 34/35 was demonstrated in 23 of the lesions (50%), and antibodies to herpes simplex virus types 1 and 2 were found in 74% and 65% of the serum samples tested, respectively. both human papillomavirus deoxyribonucleic acid and herpes simplex virus type 2 antigen were detected in 19 cases (41%). ...19882834955
comparison of western blot (immunoblot) and glycoprotein g-specific immunodot enzyme assay for detecting antibodies to herpes simplex virus types 1 and 2 in human sera.sera from patients with culture-proven genital herpes infections were tested for herpes simplex virus type 1 (hsv-1)- and hsv-2-specific antibodies by both a western blot (immunoblot) technique (wba) and immunodot enzyme assays (ieas) specific for hsv-1 or hsv-2 glycoprotein g (gg). of 137 serum samples tested, none was mistyped by either wba or iea. both tests were most sensitive with sera obtained at least 21 days after onset of primary hsv-2 infections or sera drawn during recurrent hsv-2 gen ...19882835389
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