TitleAbstractYear(sorted ascending)
disease and latency characteristics of clinical herpes virus isolated after acyclovir therapy.herpes simplex virus (hsv) type 1 from a bone marrow transplant recipient and hsv type 2 from a patient with genital herpes infection were examined for sensitivity to acyclovir after both patients received therapy with the drug. a 38- and 83-fold shift in sensitivity was detected in association with a marked decrease in viral thymidine kinase activity in isolates from both patients. the resistant hsv-1 isolate was approximately 900 times less neurovirulent to balb/c mice but had similar cutaneou ...19826285725
spectrum of sensitivity of acyclovir of herpes simplex virus clinical isolates.hsv-1 and hsv-2 clinical isolates were tested for their in vitro sensitivity to acyclovir. the median id50 for 32 genital hsv-2 isolates was 0.215 micrograms/ml and this was not significantly higher than a median value of 0.125 micrograms/ml for 28 oral hsv-1 isolates (p = 0.08). a wider range of id50s was observed with the hsv-2 isolates compared with the hsv-1 isolates. the concentration of drug required to inhibit virus replication by 90 and 99 percent was approximately 10- and 100-fold great ...19826285726
mechanism of action and selectivity of acyclovir.acyclovir, an acrylic purine nucleoside analog, is a highly potent inhibitor of herpes simplex virus (hsv), types 1 and 2, and varicella zoster virus, and has extremely low toxicity for the normal host cells. this selectivity is due to the ability of these viruses to code for a viral thymidine kinase capable of phosphorylating acyclovir to a monophosphate; this capability is essentially absent in uninfected cells. the acyclovir monophosphate (acyclo-gmp) is subsequently converted to acyclovir tr ...19826285736
efficiency and selectivity of (e)-5-(2-bromovinyl)-2'-deoxyuridine and some other 5-substituted 2'-deoxypyrimidine nucleosides as anti-herpes agents.a number of novel 5-substituted 2'deoxypyrimidine nucleosides exhibited antiviral activity against herpes simplex virus type 1 strain v3 (hsv-1-v3) when assayed under one-step conditions in primary human lung fibroblast j(phlf) cell cultures, and compared with the reference compounds cytosine arabinoside (ara-c), 5-iodo-2'-deoxyuridine (iudr), and 5-iodo-5'amino-2',5'-dideoxyuridine (aiu). the most effective of these were (in order of decreasing activity): (e)-5-(2-bromovinyl)-udr (brvudr) great ...19826285818
macrophage migration inhibition as a correlate of cell-mediated immunity to herpes simplex virus type 2 in mice.mice inoculated intraperitoneally or intravenously with herpes simplex virus type 2 (hsv-2) develop a focal necrotizing hepatitis. the livers show expanding foci of necrosis and increasing virus content during the first days of the infection with maximal titers achieved on day 3. the clearance of virus from the organ is manifest from day 4 onward with the most dramatic fall in virus content occurring between days 4 and 5. the development of immunity during the course of infection was assessed by ...19826286467
intratypic and intertypic specificity of lymphocytes involved in the recognition of herpes simplex virus glycoproteins.cytotoxic t lymphocytes (ctl) were generated in c57bl/6 mice with herpes simplex virus type 1 (hsv-1) (strains kos, 17, hfem, and mp) and hsv-2 (strains 186, g, and gp6). effector lymphocytes were tested for cytotoxicity against syngeneic hsv-1- and hsv-2-infected cells in a 5-h 51cr release assay. hsv-1 strain hfem was found to induce ctl efficiently only when 100-fold more virus was used as compared with hsv-1 strains kos, 17, and mp. all hsv-1 and hsv-2 strains induced cross-reactive populati ...19826286488
identification and typing of herpes simplex viruses with monoclonal antibodies.monoclonal antibodies which reacted with type-specific antigens of herpes simplex virus type 2 or with antigens shared by herpes simplex virus types 1 and 2 were used in an indirect immunofluorescence assay to type virus isolates and to detect viral antigens in cells obtained from herpetic lesions. complete concordance was obtained for 42 isolates typed by endonuclease restriction analysis of viral dna and by indirect immunofluorescence with monoclonal antibodies. examination of a limited number ...19826286719
failure of 2-deoxy-d-glucose in the treatment of experimental cutaneous and genital infections due to herpes simplex virus.the effectiveness of 2-deoxy-d-glucose (2-dg) was evaluated in the treatment of cutaneous infections with herpes simplex virus type 1 (hsv-1) in mice and genital infections with hsv type 2 (hsv-2) in mice and guinea pigs. groups of mice were inoculated in the lumbosacral or orofacial area with hsv-1 and treated topically three times a day with 0.2% or 0.5% 2-dg solution beginning 3 hr after inoculation. no effect on skin lesions, mortality, or latency was observed. mice were inoculated intravagi ...19826286785
genital herpes in guinea pigs: pathogenesis of the primary infection and description of recurrent disease.guinea pigs inoculated intravaginally with herpes simplex virus type 2 (hsv-2) developed a self-limiting infection characterized by vesiculo-ulcerative lesions on the external genital skin, urinary retention, and hindlimb paralysis. infection rarely resulted in death. virologic, histologic, and immunoperoxidase data suggested the following scheme for viral pathogenesis: initial replication in the introitus, vagina, and bladder; spread via sensory nerves to the lumbosacral dorsal root ganglia and ...19826286797
transfection with the isolated herpes simplex virus thymidine kinase genes. ii. evidence for amplification of viral and adjacent cellular dna sequences.we have previously described several series of biochemical transformation experiments in which small defined portions of herpes simplex virus (hsv-1 and hsv-2) dna encompassing the thymidine kinase (tk) gene were introduced into ltk- cells by the calcium transfection procedure. the presence of authentic virus tk enzyme in several subcloned cell lines derived from these experiments was confirmed by either the specific incorporation of [125i]iododeoxycytidine into their nuclei or the inhibition of ...19826286848
clinical and virological features of herpes genitalis in japanese women.we report herein clinical and virological findings in 35 japanese women with a confirmed herpes genitalis. the frequency of herpes genitalis was 0.24% in our obstetrical and gynecological outpatient clinic, while that in cancer screening clinics was 0.01%. eighty percent of all patients were between 20 and 49 years of age (median age of 38.5 years). genital herpes was detected more frequently in summer and autumn. virus isolations were carried out in 20 patients and 13 were positive (65.0%). of ...19826287795
augmented immunogenicity of lewis lung carcinoma by infection with herpes simplex virus type vitro, llt cells sustain hsv-2 replication without evidence of lysis. simultaneously, multiplication of the cells is stimulated. these xenogenized cells were tested for their immunopotentiating capacity: three-step immunization with xenogenized viable cells conferred significantly augmented transplantation resistance to a challenge graft with 4 x 10(4) intact llt cells. latency periods preceding tumor formation were increased and 15% of the mice failed to form primary tumors. metastasis was l ...19826288391
early and delayed shut-off of host protein synthesis in cells infected with herpes simplex virus.a mutant of herpes simplex virus type 1 [hsv-1(hfem)], tsb7, appears to have two temperature-sensitive functions. one is required during the first hour of infecting a cell (suggesting that it is performed by a virion protein) and the other is the non-essential function of "early shut-off" of cellular protein synthesis, which is mediated by a virion protein. the latter function remained temperature-sensitive in a revertant virus (rc2) grown at the non-permissive temperature (39 degrees c). howeve ...19826288847
animal studies on the efficacy of vaccination against recurrent herpes.guinea pigs, latently infected with herpes simplex virus type 2 (hsv 2), were immunized with an hsv 2 vaccine derived from extracts of infected cells and either formol inactivated or detergent treated. although such postinfection vaccination could significantly increase the titers of neutralizing antibodies, it had no effect on the course of recurrent herpes in these animals.19826289061
neutralization of herpes simplex virus by antibody in amniotic fluid.the ability of amniotic fluid to neutralize herpes simplex virus type 2 (hsv-2) was quantitated and compared with the serum neutralization titer in 158 pregnant women. neutralizing activity was expressed as the ability of 0.1 ml of amniotic fluid to reduce the expected number of plaque-forming units (pfu) in the inoculum by 99%. all amniotic fluid samples from 32 women with serum titers of 1:40 or greater neutralized 5 pfu or more; at term, 96% of these fluid samples neutralized 50 pfu or more, ...19826289206
clinical and virologic studies on female genital herpes.ninety women with genital herpes were studied clinically and virologically. from a clinical standpoint, these patients could be classified into 3 clinical types--acute, recurrent, and provoked. when herpes simplex virus (hsv) strains isolated from these patients and asymptomatic virus secretors were typed, 36 strains were determined to be type 1 (hsv-1) and the remaining 54 to be type 2 (hsv-2). the acute type was caused by primary infection of hsv-1 or hsv-2 as well as by infection of hsv-2 in ...19826289208
[humoral immunity to herpes simplex virus type 2 in cervical cancer].the analysis of the results of serological studies revealed differences between the number of positively reacting sera from patients with cervical carcinoma and from normal women. the activity of complement-fixing and virus-neutralizing antibodies in the patients' sera and the frequency of their detection were higher than in the sera from normal subjects. these results in combination with other methods of laboratory examinations may be used for early detection of cervical carcinoma as well as fo ...19826289530
the effect of prostaglandins on the multiplication and cell-to-cell spread of herpes simplex virus type 2 in vitro.herpes simplex virus type 2 (hsv-2) after infecting an individual has the ability to remain latent in nervous tissues. the factors that control herpesvirus infection, latency, and reactivation are poorly understood. fever, menstruation, emotional stress, exposure to sunlight, and surgical resection have been associated with activation of latent herpes. the situations which activate latent herpes are associated with a local or systemic rise in prostaglandins. the data presented show that prostagl ...19826289665
protection against lethal challenge of balb/c mice by passive transfer of monoclonal antibodies to five glycoproteins of herpes simplex virus type 2.monoclonal antibodies secreted by six hybridomas and recognizing antigenic sites on glycoproteins gc, gab, gd, ge, and gf of herpes simplex virus type 2 were examined for their ability to protect balb/c mice from lethal infection by the virus. administration of monoclonal antibodies to individual glycoproteins intraperitoneally 3 h before footpad challenge with 10 times the 50% lethal dose of virus protected between 35 and 75% of the mice, except for one of two monoclonal antibodies recognizing ...19826290390
early interactions of herpes simplex virus with mouse peritoneal macrophages.adsorption of herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) to resident peritoneal macrophages (pm) of 4-week-old swiss albino (sa) and gr/afib mice was studied. a significantly (p less than 0.05) higher hsv-2 adsorption rate was found with pm of sa mice than with pm of gr/afib mice. of added hsv-2 65% bound to the cells of sa mice over a 120-min period versus 15% to pm of gr/afib mice. only 15 to 20% of added hsv-1 bound to pm regardless of the mouse strain. these patterns of adsorptio ...19826290397
herpetic proctitis and meningitis: recovery of two strains of herpes simplex virus type 1 from cerebrospinal fluid.a patient with simultaneous proctitis and meningitis due to herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) was extensively investigated. in both disease locations the infection was clinically evident and culture-proven. analysis by sodium dodecylsulfate-polyacrylamide gel electrophoresis of rectal isolates revealed both hsv-1 and hsv-2. the cerebrospinal fluid harbored two apparently different strains of hsv-1, one of which was shown by restriction endonuclease analysis to be identical w ...19826290573
genomic location and lack of phosphorylation of the hsv immediate-early polypeptide ie 12.herpes simplex virus type 1 (hsv-1) induces an immediate-early (ie) polypeptide ie 12. an equivalent polypeptide coded by hsv-2 which migrated slightly more slowly on sds-polyacrylamide gels was identified and designated ie 12.3. analysis of the serotype of the ie polypeptide induced by five hsv-1/hsv-2 intertypic recombinants when correlated with their genome structures showed that ie 12/ie 12.3 mapped in the region spanning the trs/us junction. unlike four other ie polypeptides induced by hsv- ...19826290593
[morphological, cytogenetic and proliferative characteristics of syrian hamster htc-2 and htc-1 cells and of the htct tumor cell line transformed by herpes simplex type 2 virus].morphological, caryological, cytoproliferative, and isoenzyme studies of syrian hamster cells htc-2 and htc-1 transformed by herpes simplex virus type 2 and a htct line of tumor cells were carried out. the hamster origin of these cell lines was established by chromosomal analysis and electrophoretic mobility of lactate dehydrogenase and glucose-6-phosphate-dehydrogenase isoenzymes. the transformed and tumor cell lines differ from normal cells by altered morphology, an aneuploid chromosome set an ...19826291246
capacity of deoxycytidine to selectively antagonize cytotoxicity of 5-halogenated analogs of deoxycytidine without loss of antiherpetic activity.enzyme kinetic studies from this laboratory (m. dobersen and s. greer, biochemistry 17:920-928, 1978) suggested that deoxycytidine could antagonize the toxicity of 5-halogenated analogs of deoxycytidine without interfering with their antiviral activity. antagonism by deoxycytidine of the toxicity of 5-chlorodeoxycytidine without impairing its anti-herpes simplex virus type 2 activity is demonstrated in the present studies. tetrahydrouridine, an inhibitor of cytidine deaminase, was utilized. the ...19826291452
genital herpes simplex infections in israel: 1973 throughout 1980.ninety-eight cases of genital herpes simplex virus infection (hsv-2) were confirmed by laboratory examinations during the years 1973-80. until 1975 there were less than five cases per year, but from 1976 the number of cases gradually increased, reaching 24 in 1980. the age distribution is typical of a sexually transmitted disease; and since the peak of susceptibility is at childbearing age, an increase in neonatal hsv infection could be expected. in 1980, laboratory testing confirmed neonatal hs ...19826292133
correlation of the virus sequence content and biological properties of cells carrying the herpes simplex virus type 2 thymidine kinase cell lines, transformed to a thymidine kinase-positive phenotype with herpes simplex virus type 2 (hsv-2) dna fragments, have been examined to determine their virus-specific dna sequence content. the results are consistent with the reported map position of the hsv-2 thymidine kinase gene. different cell lines contained different amounts of non-selected virus-specific sequences and this correlated with the ability of some cell lines to compensate the deficiencies in some temperature-sensiti ...19826292338
transfection with the isolated herpes simplex virus thymidine kinase genes. i. minimal size of the active fragments from hsv-1 and hsv-2.we have defined the minimal size and physical map locations in the genomes of both herpes simplex virus types 1 and 2 (hsv-1 and hsv-2) for dna sequences capable of conferring stable biochemical transformation under thymidine kinase (tk) selection. the experiments involved transfection of ltk- cells with either isolated virus dna fragments or cloned pbr322 plasmids containing the 3.5 kilobase (kb) bamhi-o fragment from hsv-1(mp) or the 5.6 kb sali-g fragment from hsv-2(333). mapping of restricti ...19826292348
isolation and characterisation of two strains of herpesvirus hominis type 1 from fallopian tubes.herpesvirus hominis type 1 (hsv-1) was isolated from fallopian-tube biopsies of two women who were using an intra-uterine contraceptive device; the strains were isolated on different dates and at different hospitals. both the isolates were shown to be hsv-1 by restriction-enzyme analysis, polypeptide profiles and indirect immuno fluorescence; the two isolates could not be distinguished by these tests, but the tests distinguished them from hsv-1 strain 71-15 and hsv-2 strain 333. the viruses were ...19826292427
expression of cloned herpesvirus genes. i. detection of nuclear antigens from herpes simplex virus type 2 inverted repeat regions in transfected mouse cells.three different recombinant plasmids containing the entire 15-kilobase l and s inverted repeat sequence of herpes simplex virus type 2 dna have been introduced into cultured ltk- or bsc cells by both the calcium and deae-dextran transfection procedures. in each case, after 24 h approximately 1% of the cells gave strongly positive nuclear staining when assayed by immunofluorescence with hyperimmune antisera made against early and immediate-early infected-cell polypeptides. the nuclear fluorescenc ...19826292452
glycoproteins of herpes simplex virus type 2 as defined by monoclonal antibodies.we used monoclonal antibodies reacting with glycoproteins specified by herpes simplex virus type 2 (hsv-2) to characterize the individual antigens in terms of structure, processing, and kinetics of synthesis in bhk or vero infected cells. our results provided a direct demonstration of the structural identity of the ga and gb proteins of hsv-2 as well as confirmation of the existence of type-specific and type-common domains within the gd molecule. they also show that, with the exception of gc, pr ...19826292496
herpes simplex virus glycoprotein ga/b: evidence that the infected vero cell products comap and arise by proteolysis.we recently reported (pereira et al., proc. natl. acad. sci. u.s.a. 78:5202-5206, 1981) that herpes simplex virus 1 and 2 glycoproteins, previously designated ga and gb, could not be differentiated by a bank of independently derived type-specific and type-common monoclonal antibodies. we also reported that from lysates of infected vero cells, all but one monoclonal antibody precipitated ga/b glycoproteins which had faster electrophoretic mobility than the corresponding infected hep-2 cell glycop ...19826292507
unusual serologic response to two patients to an early antigen of herpes simplex virus type 2.two patients infected with herpes simplex virus type 2 (hsv-2) who were free of recurrences over a 13-14-month period had an unusually high titer (greater than 1:48) of complement-fixing antibody to the early antigen (ag-4) of hsv-2. their response was also unusual in that it was an igg complement-fixing antibody rather than igm, which normally is found in patients with hsv-2 infection. the antibody was also present 13-14 months after the episode of hsv-2 disease, whereas in other patients, the ...19826293106
[transformation and malignant degeneration of newborn hamster cells inoculated with herpes simplex virus type 2]. 19826293203
comparison of the in vitro and in vivo anti-herpes activities of 1-beta-d-arabinofuranosylthymine and its 5' vitro and in vivo anti-herpes activities of 1-beta-d-arabinofuranosylthymine 5'-monophosphate (ara-tmp) were compared with those of 1-beta-d-arabinofuranosylthymine (ara-t). on a molar basis ara-tmp was almost as active as ara-t against six strains of herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) as monitored by a cytopathogenicity-inhibition and a plaque reduction assay in human embryonic lung fibroblast cells. when tested against experimental encephalitis in mice inoculated intrace ...19826293376
comparison of restriction endonuclease profiles of dna from local herpes simplex virus type 2 (hsv-2) strains.comparisons of restriction endonuclease profiles of dna isolated from nine genital hsv-2 strains isolated from 1973 to 1981 were made using six restriction endonucleases (bgl ii, hind iii, xba 1, eco ri, bam hi and hpa i). the only variation detected in the dna profiles was found with eco ri and bam hi, and in each case all the isolates could be classified into two groups. the difference between the two eco ri groups could be accounted for by the absence of an eco ri cleavage site from the hsv d ...19826293436
chemical interactions with herpes simplex type 2 virus: enhancement of transformation by selected chemical carcinogens and pro-carcinogens.quantitative assays for the morphological transformation of 3t3 swiss mouse cells by herpes simplex type 2 virus (hsv-2) were employed to examine the effect on cell transformation of chemical carcinogens and pro-carcinogens. the carcinogens tested were n-methyl-n'-nitro-n-nitrosoguanidine, quinacrine mustard, n-nitrosomethyl urea, urethane, and benzene. the pro-carcinogens tested were n-nitrosodimethylamine, 3-methylcholanthrene, benzo[a]pyrene, and p-dimethylaminoazobenzene. exposure of the cel ...19826293737
detection by rna blot hybridization of rna sequences homologous to the bglii-n fragment of herpes simplex virus type 2 dna.rna species, extracted at the time of peak synthesis of the alpha, beta, and gamma classes of herpes simplex virus polypeptides from lytically infected vero cells, were examined for homology to the bglii-n fragment (map units 0.58 to 0.63) of herpes simplex virus type 2 dna. by using northern blot analysis, two major and several minor polyadenylated rna species showed homology to the bglii-n fragment at times corresponding to the maximum synthesis of the beta (7 h postinfection) and gamma (12 h ...19826294326
[acquired immunologic deficiency syndrome, opportunistic infections and homosexuality. presentation of 3 cases studied in switzerland].three cases of multiple opportunistic infections in previously healthy homosexual males were observed in 3 different university hospitals in switzerland. two of the patients died. infections were multiple, with p. carinii pneumonia (3 cases), chronic mucocutaneous ulcers probably due to herpes simplex virus (2 cases), mucosal candidiasis (2 cases) and disseminated infections due to cytomegalovirus (1 case) and mycobacterium avium (1 case). all patients had depressed cellular immunity with marked ...19826294820
chromosomal organization of the herpes simplex virus type 2 genome. 19826294981
confirmation of herpes simplex virus type 2 infections in herpes-like genital lesions by a simple complement-fixation test.the presence of complement-fixing antibody to an early herpes simplex virus type 2 (hsv-2) antigen (the ag-4 antigen) was correlated with hsv-2 infection in the sera of patients with genital herpes. eighty-eight per cent of sera taken two weeks after clinical diagnosis of a primary or recurrent herpes infection in patients, confirmed to have hsv-2 by virus isolation and typing, contained the anti-ag-4 complement-fixing antibody. none of the patients with genital hsv-1 had the antibody, and only ...19826275941
investigations in the transforming activity of temperature-sensitive mutants of herpes simplex virus.transformation of rat embryo fibroblast was induced by a temperature-sensitive mutant of herpes simplex virus type 2. transformation was scored using the morphological criterion of focus formation and the cell changes that lead to the final focus are described. virus genome persistence in the transformed cell line was demonstrated by the presence of virus-specific membrane antigens and by the specificity of antibodies elicited in rabbits by inoculation of transformed cells. the cytogenetic analy ...19826280377
antibody-dependent cell-mediated cytotoxicity to herpes simplex virus-transformed cells in the course of cervical carcinoma.the antibody-dependent cell-mediated cytotoxicity assay was used to determine whether humoral antibodies from women with cervical carcinoma in the presence of normal mononuclear cells could induce cytotoxicity to hamster embryo fibroblasts, originally transformed by herpes simplex virus type 2 (hsv-2) (333-8-9), to a cervical carcinoma cell line (me-180) and to a lung carcinoma cell line (a-549). control groups consisted of 81 age-matched healthy women and 77 patients suffering from malignancies ...19826280493
immunity to herpes simplex virus type 2. iv. impaired lymphokine production during recrudescence correlates with an imbalance in t lymphocyte subsets.cell-mediated immunity (cmi) to herpes simplex virus type 2 (hsv-2) was studied in patients with recurrent disease. peripheral blood lymphocytes (pbl) obtained during recrudescence (0 to 3 days post-onset of lesion), convalescence (4 to 14 days), and quiescence (greater than 14 days), which were cultured in vitro in the presence of uv-inactivated hsv-2 (g) antigen, demonstrated similar, virus-specific lymphoproliferative responses. however, the production of the lymphokine, leukocyte migration-i ...19827045224
spinal cord and optic nerve demyelination in experimental herpes simplex virus type 2 produce a model of central nervous system (cns) demyelination, three strains of herpes simplex virus type 2 (hsv-2) were injected intracerebrally into swiss-webster outbred mice. animals developed pupillary abnormalities and leg weakness and, by light and electron microscopy, had focal lesions of primary demyelination in optic nerves and spinal cords. oligodendrocytes and astrocytes in these lesions contained herpesvirus. both mouse age and virus dose were important determinants of the rate a ...19827077361
optimal treatment of herpes simplex virus encephalitis in mice with oral acyclovir.the effect of oral or intraperitoneal administration of acyclovir was evaluated in four experimental models of herpes simplex virus (hsv) encephalitis in mice. mice were inoculated with hsv-1 or hsv-2 intracerebrally or with hsv-2 intranasally, intraperitoneally, or intravaginally. with all four routes of inoculation, oral acyclovir therapy significantly reduced mortality when started as late as 72 to 96 hours after viral challenge. intraperitoneal acyclovir was not effective in protecting mice ...19827102699
virus-specific antibodies in sera from patients with genital herpes simplex virus infection.virus-specific antibodies against a number of herpes simplex virus type 2 antigens were determined by radioimmunoprecipitation assays in sequential serum samples obtained from 12 patients with initial genital herpes simplex virus infection. the progressive appearance of antibodies to virus-specific antigens was observed; antibodies against a 130,000-molecular-weight glycoprotein complex appeared first, followed by antibodies against the major nucleocapsid polypeptide and then antibodies against ...19827118244
herpes simplex type 2 virus encephalitis presenting as psychosis.the current literature recognizes two antigenic types of herpes simplex virus, type 1 and 2. type 1 is the most common cause of sporadic necrotizing encephalitis in the united states, with a mortality rate of 30 to 70 percent, and leaves various neurologic sequelae in the survivors. herpes simplex virus type 2 had been recognized as an etiologic agent in fatal infections in neonates and a mild meningitis in adults, but its role in encephalitis in adults is less well known. we report a case of he ...19827124772
lethal encephalitis and non-lethal multifocal central nervous system demyelination in herpes simplex virus type 2 infections in mice.six-week-old swiss-webster mice were infected intracerebrally with a low dose of herpes simplex virus type 2 (hsv-2) and were studied by pathological, virological and immunological methods. one third of mice developed severe neurological disease and died during the first 31/2 weeks of infection, while the remaining two thirds survived this acute stage with relatively minor neurological signs. these survivors had multifocal demyelinative white-matter lesions in the cns, and gray-matter lesions, i ...19827150512
aseptic meningitis and herpes simplex proctitis. a case report.a male homosexual patient developed an aseptic meningitis during the course of acute ano-proctitis due to herpes simplex virus type 2 (hsv-2). aseptic meningitis (sometimes proved to be due to hsv-2) has been associated with genital but not with anal herpes. this sexually transmissible virus should be considered when patients with aseptic meningitis are investigated.19827198922
epidural analgesia in the presence of herpes simplex virus (type 2) infection. 19827201280
epidemiology of sexually transmitted diseases in the scandinavian the scandinavian countries, laws require reporting of the traditional venereal diseases, i.e., gonorrhea, syphilis, lymphogranuloma venereum (lgv), and ulcus molle, or chancroid. incidence figures for these diseases, therefore, are fairly reliable. in the scandinavian countries, lgv and chancroid are rare diseases, and the syphilis incidence has remained low. these three diseases are typically male afflictions. gonorrhea outnumbers the other diseases mentioned and at present has a nearly 1:1 ...19826958011
modulation by cyclosporin a of murine natural resistance against herpes simplex virus infection. i. interference with the susceptibility to herpes simplex virus balb/c mice which are medium-high resistant against intraperitoneal (i.p.) infection with herpes simplex virus type 2 (hsv-2) manifested a drastic increase in susceptibility to the virus when treated locally with cyclosporin a (cya) during infection. oral application of the drug had no effect on the natural resistance status. mice appeared normal 2 weeks after cya treatment with regard to their ability to resist i.p. infections. cya did not interfere with established specific immune protec ...19826980627
congenital herpes simplex virus, type 2, bilateral endophthalmitis.a set of dizygotic twins, both born prematurely, developed herpes simplex encephalitis shortly after birth. the second twin had unilateral keratoconjunctivitis and bilateral endophthalmitis and subsequently died from disseminated herpes infection. herpes simplex, type 2, was isolated from conjunctiva of both eyes, cerebrospinal fluid, nasopharynx and trachea. histologic examination of the eyes revealed bilateral endophthalmitis with a necrotizing retinitis. intranuclear inclusion bodies were dem ...19826985228
influenza type a virus-mediated adherence of type 1a group b streptococci to mouse tracheal tissue in vivo.influenza type a virus-mediated adherence of pathogenic bacteria to the mucosal surface of the respiratory tract may be one of several mechanisms whereby influenza predisposes to bacterial pneumonia. in the present study, we quantified the adherence of intranasally administered type 1a group b streptococci to the tracheal tissue of influenza type a/pr8/34 (honi) virus-infected and mock-infected mice. influenza type a/pr8/34 virus infection effected a 120-fold increase in the adherence of type 1a ...19826754623
treating viral venereal infections with the co2 laser.viral venereal infections caused by herpes simplex virus 2 and papillomavirus (wart) are epidemic diseases in the united states. unfortunately, these two infections have eluded conventional therapy to date. therefore, 306 women were treated for viral venereal diseases with the co2 laser. two hundred twenty-eight patients were treated for condyloma acuminatum and 78 for genital herpes. all the patients who underwent treatment for herpes were enrolled in an investigative protocol. since the viral ...19826819358
differential susceptibility of human neural cell types in culture to infection with herpes simplex virus.the ability of herpes simplex virus (hsv)-1 glasgow (strain 17), hsv-2 (strain hg52), temperature sensitive mutants and a thymidine kinase negative mutant of glasgow strain 17 to produce a cytopathic effect and induce viral antigen expression in primary and passaged human fetal brain cells was studied. no difference was detected between the behaviour of hsv-1 and hsv-2. cell-type-specific markers and indirect immunofluorescence were used to define unambiguously the type of individual cells in cu ...19836831193
detection of type-specific antibody to herpes simplex virus type 1 by radioimmunoassay with herpes simplex virus type 1 glycoprotein c purified with monoclonal antibody.herpes simplex virus type 1 (hsv-1) and hsv-2 specify at least four glycoproteins designated ga/gb, gc, gd, and ge. previous studies have shown that gc produced by hsv-1 is antigenically distinct from the corresponding hsv-2 glycoprotein. with the exception of gc, the glycoproteins of both serotypes share antigenic sites. standard serological assays fail to differentiate the antibody to the shared antigenic determinants from the type-specific antibody. in this paper, we describe a procedure for ...19836832831
[possibilities and limits of chemotherapy of diseases caused by herpes simplex and varicella-zoster virus].the available antiviral chemotherapeutic agents for the treatment of herpes simplex type 1 and 2 and varicella zoster virus infections are discussed in respect of their mechanism of action, clinical effects, and side effects. although effective under experimental conditions most of the antiherpetic drugs need further evaluation of clinical efficacy in controlled trials. it is possible to reduce initial herpetic pain mainly in patients with zoster by topical application of 5% 5-iodo-2'-deoxyuridi ...19836841073
protection of newborn mice against herpes simplex virus infection by prenatal and postnatal transmission of antibody.pre- and postnatally acquired protection against herpes simplex virus type 2(hsv-2) infection mediated by maternal antibody was investigated in the newborn mouse. newborn mice, 2 days old, were inoculated with hsv-2 intraperitoneally after maternal immunization with live or inactivated virus. the survival rates improved in proportion to the maternal neutralizing antibody titres. ninety-three percent of animals delivered by caesarean section from immune mothers and suckled by non-immune mothers s ...19836842184
leptomeningitis and polycaryocyte formation in the cns of rats inoculated subcutaneously with herpes simplex virus.wistar rats were inoculated subcutaneously with either type 1 (hsv1) or type 2 (hsv2) herpes simplex virus at 5 days of age. animals were killed in extremis or at the end of the 14-day observation period postinoculation. acute destructive meningoencephalitis with hemorrhage and leukocytic infiltration was observed in both groups. polycaryocytes comprised of cells of the internal granular layer of the cerebellum were observed in some animals inoculated with hsv1. these multinucleated cells appear ...19836852211
viruses and human cancer.the cause of human cancer is probably multifactorial and the role of viruses is unclear. the study of retroviruses has led to the identification of oncogenes responsible for transformation and tumor induction. human viruses associated with malignancies include the jc virus (associated with progressive multifocal leukoencephalopathy) and some adenoviruses. no human malignancies have been associated with the latter group. a number of herpes viruses of lower animals have been associated with malign ...19836870184
herpes type 2 infection with unusual generalised manifestations and delayed diagnosis in an adult male.a case of severe generalised herpes simplex type 2 infection is described in an adult male who had known exposure to herpes. the patient first complained of headache, fever and neurological symptoms, and three to six days later of conjunctivitis, severe pharyngitis, arthralgia and vesicular lesions about the body. during the first 14 days of illness, including three in hospital, the patient was diagnosed as having infection with varicella virus, vesicular stomatitis virus, or hand-foot-and-mouth ...19836875292
preferential inhibition of 5-trifluoromethyl-2'-deoxyuridine phosphorylation by 5'-amino-5'-deoxythymidine in uninfected versus herpes simplex virus-infected cells.the cytotoxic effects of 5-trifluoromethyl-2'-deoxyuridine (cf3durd) were effectively antagonized by 5'-amino-5'-deoxythymidine (5'-adthd). the antiproliferative actions of cf3durd were reduced in a dose-dependent manner by 5'-adthd in both hela and vero cells. in addition, the ability of cf3durd to kill hela cells (95% at 1 microm and 99% at 3 microm), as measured by cloning efficiency, was ablated entirely by 5'-adthd (300 microm). in contrast, the inhibition of herpes simplex virus type 2 (hs ...19836408395
dna sequence of the herpes simplex virus type 2 glycoprotein d gene.we describe a 1635-bp herpes simplex virus type 2 (hsv-2) dna sequence containing the entire coding region of glycoprotein d (gd-2). the amino acid sequence of gd-2, deduced from the nucleotide sequence, was compared to that of the analogous herpes simplex virus type 1 (hsv-1) glycoprotein (gd-1). the two glycoproteins are 85% homologous and contain highly conserved regions of as much as 49 amino acids in length. comparison of dna sequences upstream from gd-1 and gd-2 coding regions identified p ...19836323270
herpetic whitlow: report of a case with multiple recurrences.a case of recurrent herpetic whitlow is discussed. the patient, a dental assistant at the time of her initial infection, developed herpetic whitlow caused by herpes simplex virus type 2 (hsv-2). she has subsequently experienced nine recurrent infections with lesion spread, scarring, and persistent paresthesia. the severity of the most recent recurrences appears to have diminished, and prodromal sensations, that is, increased local sensitivity, have occurred without the subsequent development of ...19836576289
efficacy of the acyclic nucleoside 9-(1,3-dihydroxy-2-propoxymethyl)guanine against primary and recrudescent genital herpes simplex virus type 2 infections in guinea pigs.the acyclic nucleoside 9-(1,3-dihydroxy-2-propoxymethyl)guanine (dhpg) was evaluated for its efficacy in protecting guinea pigs from primary and recrudescent infections of herpes simplex virus type 2. vaginally infected guinea pigs were treated twice a day with dhpg at 25 mg/kg per dose for 3 weeks. subcutaneous doses were started 3 h, 24 h, or 5 weeks after virus inoculation. treatment starting at 3 or 24 h reduced the severity of the primary infection by greater than 70% when lesions were grad ...19836607031
cell surface alterations induced by the acycloguanosine (acg) resistant and sensitive strains of herpes simplex virus type 2 (hsv-2) in the presence of acg and tunicamycin.hsv-2-acg, a mutant of herpes simplex virus type 2, which is resistant to acycloguanosine and also capable of inducing fusion of bsc1 cells, was isolated. the viral-induced surface changes in these cells were studied using scanning electron microscopy. the formation of gigantic fused cells with increased number of microvilli and virus-like particles, extensively covering the infected cell surface, were investigated. tunicamycin (50 micrograms/ml), an inhibitor of glycosylation of polypeptides, b ...19836322282
rapid diagnosis of varicella-zoster virus infection by detection of viral deoxythymidine kinase in serum and vesicle fluid.a sensitive enzyme assay utilizing [125i]iododeoxyuridine as the substrate and ctp as the phosphate donor in combination with isozyme-specific antisera was used for direct detection and typing of herpesvirus deoxythymidine kinase (dtk) in clinical specimens. an investigation of 16 coded vesicle fluid specimens, taken in connection with varicella-zoster virus (vzv) and herpes simplex virus infections, revealed viral dtk activity in 14 samples. all positive samples except one were taken within 5 d ...19836339548
current status and prospects for oral acyclovir treatment of first episode and recurrent genital herpes simplex virus.herpes simplex genitalis is an increasingly common primary or recurrent hsv infection. acyclovir (acv) has been found to diminish virus shedding and clinical symptoms in patients with first episode genital hsv when given topically or intravenously. as most patients with first episode disease do not require hospitalization and recurrent disease is usually less severe than the primary infection, out-patient treatment forms the basis of practical management. acyclovir is absorbed orally with plasma ...19836355052
lysis of herpesvirus-infected cells by macrophages activated with free or liposome-encapsulated lymphokine produced by a murine t cell hybridoma.thioglycolate-induced mouse peritoneal macrophages were activated in vitro by the lymphokine designated macrophage-activating factor (maf) produced by a murine t cell hybridoma to lyse herpes simplex virus type 2 (hsv-2)-infected murine target cells. comparison of uninfected balb/c 10e2 cells with hsv-2-infected 10e2 cells showed that macrophages activated with maf selectively destroyed hsv-2-infected cells and left uninfected cells unharmed, as measured by an 18-h 51cr-release assay. in contras ...19836358037
expression and cellular compartmentalization of a herpes simplex virus type 2 protein (icp 10) in productively infected and cervical tumor cells.antiserum to icp 10, a herpes simplex virus type 2 (hsv-2) protein that is expressed in cells neoplastically transformed by viral dna sequences within the bgl ii/hpa i cd fragment, specifically precipitates the icp 10 protein from hsv-2 infected cells and stains cells infected with hsv-2 for 4 to 16 hrs by indirect immunofluorescence. at 4 hr post infection (p.i.), the staining is primarily perinuclear, while at 16 hr p.i., it is cytoplasmic and intranuclear. compartmentalization studies indicat ...19836320993
therapeutic activities of 1-(2-fluoro-2-deoxy-beta-d-arabinofuranosyl)-5-iodocytosine and -thymine alone and in combination with acyclovir and vidarabine in mice infected intracerebrally with herpes simplex virus.the therapeutic effectiveness of two new antiviral agents, 1-(2-fluoro-2-deoxy-beta-d-arabinofuranosyl)-5-iodocytosine and 1-(2-fluoro-2-deoxy-beta-d-arabinofuranosyl)thymine, was compared with that of acyclovir and vidarabine. in mice inoculated intracerebrally with high 50% lethal doses of herpes simplex virus type 2, nontoxic intraperitoneal or oral treatments with the two new fluorinated antiviral agents were highly effective in reducing mortality. the two drugs were also effective when trea ...19836625559
topography of remyelinated chronic spinal cord lesions in herpes simplex virus type 2 infections of mice.six-week-old outbred mice were infected intracerebrally with a low dose of the ms strain of herpes simplex virus type 2. about 1% of neurologically abnormal survivors developed paralysis or severe leg weakness during the first three weeks of infection. weakness persisted with little subsequent improvement. five to 8 months later, 4 such mice were killed, and each spinal cord was examined in epon sections in a series of transverse sections along their lengths. all cords had 2 or more major white ...19836655486
increase in laboratory-confirmed cases of herpes genitalis and neonatal herpes infections in israel.jewish women have been considered to be at low risk for genital herpes simplex virus type 2 (hsv-2) infections. during the period 1973 to 1981, genital herpes simplex infections were laboratory confirmed in 129 cases (81 women and 48 men). until 1976, only sporadic cases were reported to our laboratory. since then, the number of cases has gradually increased, reaching 31 new reported cases in 1981. the age distribution was typical for a sexually transmitted disease, with the peak of infection at ...19836662679
congenital absence of scalp skin and herpes simplex virus. a case report.a male infant, born at 40 weeks' gestation of a 27-year-old mother, presented at delivery with three scalp lesions, 2 to 4 cm in diam, covered with necrotic tissue and devoid of hair. the infant was born by natural vertex delivery within 2 hours of membrane rupture; birth weight was 3,300 g and apgar score 10. there were no other skin lesions, and physical and neurological examinations were normal. herpes simplex virus type 2 was isolated from the lesions and the mother's cervix. high titers of ...19836662680
antiviral and antitumor compounds from tunicates.tunicates provide a rich source of biologically active compounds with potentially useful medicinal properties. the most interesting compounds identified thus far are the didemnins, depsipeptides from a caribbean trididemnum species, which are potent inhibitors of l1210 leukemia cells in vitro and are also active in vivo against p388 leukemia and b16 melanoma. in addition the didemnins inhibit growth of a variety of rna and dna viruses in vitro and protect mice infected intravaginally with herpes ...19836681537
co-existence of malignant squamous cells and herpes simplex virus type 2-infected cells.a case is presented in which malignant squamous cells and herpes virus infected cells were recognized concomitantly at routine cytologic examination for detection of cervical cancer. further examinations on admission revealed stage ib carcinoma of the cervix and characteristic pathologic changes of herpetic infection in the tumor cell nests. virus was isolated from the cancerous tissues in the ectocervix following radical hysterectomy, and was identified as herpes simplex virus type 2 (hsv-2). t ...19836681607
[the autoinoculation test. false-positive reactions caused by herpes simplex virus].the autoinoculation test has been used as a diagnostic procedure in patients with chancroid-suspected genital ulcerations. in four of 13 patients with a positive autoinoculation test, herpes simplex virus type 2 was isolated from the primary lesions. in two of the cases this virus was also isolated from the inoculation site, and one patient developed a recurrent herpetic eruption here. two patients were proven to have chancroid. three of the remaining seven patients developed recurrent herpes ge ...19836682839
carbocyclic arabinofuranosyladenine (cyclaradine): efficacy against genital herpes in guinea pigs.carbocyclic arabinofuranosyladenine (cyclaradine), a novel nucleoside analog with such desired features as hydrolytic and enzymatic stability, adenosine deaminase resistance, and low systemic toxicity, inhibited the replication of herpes simplex virus types 1 and 2. the 5'-methoxyacetate prodrug form exhibited significant efficacy in the topical treatment of genital infections by herpes simplex virus type 2.19836684328
vaginal contraception with gossypol: a clinical study.a clinical study concerning the vaginal contraceptive efficacy of gossypol acetic acid was performed. fifteen women who had undergone tubal sterilization volunteered for the study. the effect of vaginal gossypol-containing gel on spermatozoa was determined by postcoital tests performed in subjects without and after using gossypol gel. after gossypol application, the number of spermatozoa found in cervical mucus was greatly decreased and, in eleven of the fifteen women, all spermatozoa seen were ...19836684533
antiviral activities of cloned human leukocyte interferons against herpes simplex virus type 2 infections of mice.human alpha-interferons (ifn-alpha s) made in bacteria were examined for antiviral activity against herpes simplex virus type 2 (hsv-2) infections of mouse l-cells in vitro, and acute cervicovaginal and lethal systemic hsv-2 infections of balb/c mice. the recombinant dna-derived hybrid interferon ifn-alpha ad(bgl) showed pronounced antiviral activity in vitro, exceeding the activity of either of the parental subtypes ifn-alpha a and ifn-alpha d and that of the other hybrids ifn-alpha ad(pvu) and ...19836684674
lack of efficacy of amp against recrudescent genital herpes infections in guinea pigs.amp was evaluated for its efficacy against herpes simplex virus type 2 genital infections in guinea pigs. vaginally infected animals were treated twice a day with amp at 50 mg/kg per dose for 2 weeks. subcutaneous doses were started 10 weeks postinfection. amp treatment did not reduce the number of animals with recrudescent episodes when compared with saline-treated controls. in addition, amp treatment did not reduce either the number of lesions or the duration of the recrudescent episodes which ...19836685993
evaluation of prodrugs of 9-beta-d-arabinofuranosyladenine for therapeutic efficacy in the topical treatment of genital herpesvirus infections in guinea pigs.prodrugs of the antiviral agent 9-beta-d-arabinofuranosyladenine (araa), which were more effective than the parent compound in penetrating vaginal membranes in vitro, were synthesized and examined for efficacy in the topical treatment of genital infections with herpes simplex virus type 2 in female guinea pigs. treatment with 10% araa-5'-monophosphate or 10% araa-5'-monovalerate twice a day for 7 days, starting 6 h after intravaginal inoculation with virus, completely aborted the primary infecti ...19836686429
effect of cp-20,961 on genital herpes in guinea pigs.cp-20,961 (n,n-dioctadecyl-n',n'-bis(2-hydroxyethyl)propanediamine) has been reported to be an interferon inducer, an adjuvant and a macrophage activator. in the present study, this compound was used therapeutically and prophylactically to treat genital herpes simplex virus type 2 (hsv-2) infections in guinea pigs. a significant decrease in the incidence of clinical lesions (p less than 0.05) was observed in animals treated intravaginally with 20 mg cp-20,961 (two doses each containing 10 mg) pr ...19836686755
a herpesvirus antigen in human premalignant and malignant cervical biopsies and explants.cervical biopsies and explant cultures from patients with squamous metaplasia, various grades of dysplasia, carcinoma in situ (cis), and invasive squamous cell carcinoma were screened for vp143, an early nonstructural polypeptide of herpes simplex virus type 2 (hsv-2), vp143 was identified in 31% of biopsies exhibiting severe dysplasia, 29% with cis, and 41% with invasive squamous cell carcinoma. similar results were obtained when explants derived from these biopsies were examined for vp143. the ...19836295165
immunity to herpes simplex virus type 2. v. risk of recurrent disease following primary infection: modulation of t-cell subsets and lymphokine (lif) production.phenotypic and functional characteristics of peripheral blood lymphocytes (pbl) from 133 patients (87 female; 46 male) with documented primary or recurrent genital hsv-2 infection were determined by serologic testing and cmi [lymphocyte blastogenesis and lymphokine (lf)] evaluation. primary infection was followed by the development of virus-specific immune memory as defined in vitro by lymphocyte blastogenesis and the development of neutralizing antibody. virus-specific lif production was detect ...19836209056
changes in the vaginal cytology of the guinea pig induced by herpes simplex virus.the vaginal cytology of guinea pigs with genital herpes simplex virus type 2 (hsv-2) infection was examined during acute and latent infection. cytologic changes similar to those observed in humans with hsv-2 genital infections were observed in the guinea pigs during acute infection. during latent infection, some animals continued to have abnormal cytology. the severity of the changes was reduced after treatment with phosphonoformic acid during acute infection.19836223477
immunity to herpes simplex virus type 2: recurrent lesions are associated with the induction of suppressor cells and soluble suppressor factors.cell-mediated immunity to herpes simplex virus type 2 was investigated in infected inbred strain 13/n guinea pigs with (45%), and without, a history of recurrent herpetic disease (a. d. donnenberg, e. chaikof, and l. aurelian, infect. immun. 30:99-109, 1980). induction of suppressor cells capable of reducing the proliferative response of herpes simplex virus type 2-stimulated immune lymphoid cells was demonstrated in spleen cells from animals with a history of recurrent disease at recrudescence ...19836227561
a comparative analysis of restriction enzyme digests of the dna of herpes simplex virus isolated from genital and facial lesions.the restriction endonuclease (re) cleavage patterns of the dna of herpes simplex virus (hsv) genital isolates from two geographically distinct groups and from one group of facial isolates were examined. eleven of 21 genital isolates from females, 1 of 27 genital isolates from males and all 17 of the facial isolates were hsv type 1 (hsv-1). the groups of isolates of the same serotype could not be distinguished by significant differences in the frequency of variable restriction endonuclease sites ...19836300289
herpes simplex virus. an expanding relationship to human cancer.herpes simplex virus (hsv) causes a wide spectrum of infectious disease in humans. during the past 15 years, evidence has accumulated indicating that, in addition, hsv may be a cause of human cancer, particularly cervical cancer. the research on hsv has taken five directions. first is detection of previous infection with hsv 2. this was the earliest approach and was performed largely with serologic assays. studies showed a higher incidence of infection with hsv-2 in cervical cancer patients as c ...19836300393
use of monoclonal antibodies against two 75,000-molecular-weight glycoproteins specified by herpes simplex virus type 2 in glycoprotein identification and gene mapping.we produced two monoclonal antibodies that precipitate different glycoproteins of similar apparent molecular weight (70,000 to 80,000) from extracts of cells infected with herpes simplex virus type 2. evidence is presented that one of these glycoproteins is the previously characterized glycoprotein ge, whereas the other maps to a region of the herpes simplex virus type 2 genome collinear with the region in herpes simplex virus type 1 dna that encodes gc.19836300459
herpes simplex virus proctitis in homosexual men. clinical, sigmoidoscopic, and histopathological features.acute herpes simplex virus (hsv) infection was detected in 23 of 102 consecutively examined, sexually active male homosexuals who presented with anorectal pain, discharge, tenesmus, or hematochezia, as compared with 3 of 75 homosexual men without gastrointestinal symptoms (p less than 0.01). findings that were significantly more frequent in men with hsv proctitis than in men with proctitis due to other infectious causes included fever (48 per cent), difficulty in urinating (48 per cent), sacral ...19836300674
[level of cyclic nucleotides (camp and cgmp), atpase activity and calcium ion transport in hamster lymphocytes during tumor growth].the biochemical systems of lymphocytes of the peripheral blood and lymph nodes of hamsters with a herpes simplex (type 2) virus--induced transplantable tumor rapp hsv2 were studied. the camp concentration in tumor-bearing hamsters was 1.5-2 times higher, while cgmp level--2.2 times lower than in control. this was matched by a rise in the level of transport atpases in the lymphocytes of experimental animals. concanavalin a had practically no effect on the uptake of ca2+ by lymphocytes of tumor-be ...19836301159
replication of non-respiratory viruses in tracheal organ cultures.employing hoorn's technique, tracheal explant cultures were set from adult hamsters, rabbits and human foetuses. to determine the susceptibility of these cultures they were infected with nine different mainly non-respiratory viruses. assay of virus was carried out in susceptible cell lines. the results of these studies indicated that herpes simplex type i (hsv-1) and vaccinia viruses multiplied in these cultures and caused ciliostasis. herpes simplex type 2 (hsv-2) although multiplying in all, p ...19836301520
herpesvirus.herpesvirus (hsv) infection of the genital tract is a sexually transmitted disease that is increasing at an epidemic rate. 2 types of virus, type 1 (hsv-1) and type 2 (hsv-2) have been identified, of which hsv-2 is the major cause of genital and neonatal infection. type 2 herpes infections may be the 2nd most common venereal disease in the us. more than 60% of the adult us population has antibodies to hsv, and socioeconomic factors have been found to influence the incidence of hsv infection. ...19836301717
enzyme-linked immunosorbent assay for detection and typing of herpes simplex enzyme-linked immunosorbent assay for herpes simplex virus was tested using commercially available peroxidase-conjugated and unconjugated rabbit antibodies to herpes simplex virus type 1 and type 2 (dako immunoglobulins a/s, copenhagen, denmark). one hundred and thirty-seven clinical specimens from vesicles and superficial cutaneous lesions were tested and the results compared with virus isolation. in addition 210 herpes simplex virus isolates were typed. forty-four specimens yielded herpes s ...19836301810
viral genes modify herpes simplex virus latency both in mouse footpad and sensory ganglia.herpes simplex virus type 2 wild-type and 13 temperature-sensitive mutants have been examined for their ability to be recovered from latently infected biozzi mice. after inoculation into the rear footpad, virus could be recovered from both the dorsal root ganglia (drg) and the footpad (fp) at the site of inoculation (wt, ts 1, ts 2, ts 4, ts 6, ts 7 and ts 8), or from the drg only (ts 11 and ts 13), or from fp only (ts 3, ts 9, ts 12). two mutants (ts 5 and ts 10) have not been recovered from ei ...19836302212
herpes simplex virus non-structural proteins. iii. function of the major dna-binding protein.the herpes simplex virus type 2 major dna-binding protein has been functionally characterized using temperature-sensitive mutants in the complementation group 2-2. the mutants were shown to be defective in the dna-binding protein gene by mapping the mutants to the area of the genome known to code for the protein, and by demonstrating alterations in the major dna-binding protein induced in mutant-infected cells. the mutants were shown to be defective in the replication of virus dna. the nature of ...19836302216
9-[(1,3-dihydroxy-2-propoxy)methyl]guanine: a new potent and selective antiherpes agent.the synthesis of a new acyclic analogue of deoxyguanosine, 9-[(1,3-dihydroxy-2-propoxy)methyl]guanine (dhpg, 1), is described starting from epichlorohydrin via condensation of 2-o-(acetoxymethyl)-1,3-di-o-benzylglycerol (5) with n2,9-diacetylguanine (6). in vitro studies indicate that dhpg is a potent and broad-acting (herpes simplex virus types 1 and 2, cytomegalovirus, and epstein-barr virus) antiherpetic agent. in vivo studies indicate its lack of toxicity [ld50 (mice) = 1-2 g/kg, ip] and its ...19836302255
unique spectrum of activity of 9-[(1,3-dihydroxy-2-propoxy)methyl]-guanine against herpesviruses in vitro and its mode of action against herpes simplex virus type 1.a guanosine analog, 9-[(1,3-dihydroxy-2-propoxy)methyl]guanine (dhpg), was found to inhibit herpes simplex virus type 1 (hsv-1), herpes simplex virus type 2, cytomegalovirus, and epstein-barr virus replication by greater than 50% at concentrations that do not inhibit cell growth in culture. the potency of the drug against all of these viruses is greater than that of 9-[(2-hydroxyethoxy)methyl]guanine (acyclovir). dhpg was active against hsv-1 growth during the early phase of virus replication an ...19836302704
Displaying items 601 - 700 of 7420