oculogenital transmission of type 2 herpes simplex virus in adults.type 2 (genital) herpes simplex virus (hsv-2) was isolated from three patients (two adults and an 11-year-old girl) with acute ocular infections. two of these patients had acute blepharoconjunctivitis and one had acute keratoconjunctivitis. genital herpes infections had preceded the eye infections in the two adults. this was not the case in the 11-year-old, but she had been in close contact with her sister who had apparently had the genital disease. the study strongly suggests transmission of hs ...1976185732
type 1 and type 2 herpes simplex virus in corneal cell cultures.the pathogenicity of herpes simplex virus (hsv) in primary cultures of epithelia, stromal, and endothelial cells of rabbit corneas was studied at various incubation temperatures (30 degrees, 36 degrees, and 40 degrees c). we tested three strains each of type 1 (hsv-1) and type 2 (hsv-2). at all three temperatures, the epithelial cells appeared to be more susceptible to both hsv-1 and hsv-2 than the stromal and endothelial cells. in general, less hsv-1 was requered than hsv-2 to infect the same t ...1976185735
a new method for the isolation of herpes simplex virus type 2 dna. 1976185791
antiviral effect of pyran against systemic infection of mice with herpes simplex virus type 2.the immunomodulator pyran markedly protected 5-week-old mice from lethal intravenous infection with herpes simplex virus type 2. the 50% lethal dose was increased almost 100-fold in pyran-treated mice as compared with controls. although the protection was not as marked in older mice (10 and 16 weeks old), there was a significant increase in mean survival time. when the pathogenesis of herpesvirus disease was monitored in control and drug-treated mice, the effect of pyran was most evident in the ...1976185945
effects of cytochalasin and alkaloid drugs on the biological expression of herpes simplex virus type 2 dna. 1976186287
plaque dissociation of herpes simplex viruses: biochemical and biological characters of the viral variants.polycaryocytogenic (p) and non-polycaryocytogenic, or aggregating (a), stable variants were selected from a herpes simplex virus type 1 (hsv-1) and from a herpes simplex virus type 2 (hsv 2) which had not been deliberately exposed to known mutagenic agents. the p variant of hsv-1 (fp) differed from the a variant (fa) in polypeptides and glycoprotein patterns, but no gross differences were evident between the two variants of hsv-2 (gp and ga). each p variant proved more 'specific' than each a var ...1975186433
studies on kinetics of dna synthesis in cv-1 cell cultures infected with different multiplicities (moi) of herpes simplex virus type 2 (hsv-2).infection of cv-1 cell cultures with hsv-2 at moi 5 respectively 1pfu/cell resulted in markedly different patterns of dna-synthesis. isolation and separation of cellular and viral dna on cscl equilibrium density gradients during the "late" phase after infection with 5pfu/cell revealed a rapid increase in synthesis of virus-specific dna (which banded at density q25 = 1.729 g/ccm) while synthesis of host cell dna (banding at 1.705 g/ccm) was constantly inhibited. 15 hours after infection and incub ...1976186742
herpesviruses and cancer in man and subhuman primates.because there is strong evidence for the involvement of epstein-barr virus in the etiology of burkitt's lymphoma and nasopharyngeal carcinoma, we have discussed the relationship of epstein-barr virus to these two diseases in the context of geographic distribution, pathology, epidemiology, genetics, immunovirology, and biochemistry. we have also discussed the relationship of epstein-barr virus to other diseases, both malignant and non-malignant. although the etiologic relationship of herpes simpl ...1976187259
reaction of antigens isolated from herpes simplex virus-transformed cells with sera of squamous cell carcinoma patients.antigens isolated from herpes simplex virus type 1, herpes simplex virus type 2, or cytomegalovirus-transformed hamster cells were tested against 66 sera from non-cancer individuals or patients with different types of cancer. by use of the microcomplement fixation procedure to quantify all antigen-antibody interactions, it was observed that 94% (p less than 0.001) of all sera from patients with squamous cell carcinoma reacted with antigens from herpes simplex virus type 1-transformed cells, whil ...1976187320
comparison of dna polymerase activities induced by herpes simplex virus types 1 and 2.hsv-2-induced a salt-stimulated dna polymerase activity in hep-2 cells at similar levels to that induced by hsv-1 virus at multiplicites of infection from 2 to 20 pfu/cell. de novo synthesis of protein and mrna was required for dna polymerase induction by both types. dna polymerase activity of three strains of each type was compared by response to various salt concentrations and by heat inactivation. strain-dependent, but not type-dependent differences were found by both tests. considerable cros ...1975187552
lasar flow cytophotometric immunoperoxidase detection of herpes simplex virus type 2 antigens in infected cultured human cells.human cells in culture (hep-2) were infected with herpes simplex virus type 2 (hsv-2) at multiplicities of infection varying from 0.2 to 10, and fixed 6, 12, 18 and 24 hr after infection. infection-related antigens were detected by an indirect double antibody (peroxidase conjugated goat anti-rabbit to rabbit anti-herpes simplex virus type 2) immunoenzymatic staining reaction that rendered infection-related antigens visible by light microscopy. a corresponding series of laser flow cytophotometric ...1976187690
immune interactions with cells infected with herpes simplex virus: antibodies to radioiodinated surface antigens.lactoperoxidase-catalyzed radioiodination was used to study reactions between surface antigens and antibodies on bhk-21 cells infected with hsv-1 and hsv-2. isolation of iodinated surface antigens was achieved by indirect immune precipitation of triton x-100 disrupted cells with antisera to hsv and igg. analysis of immune precipitates by polyacrylamide gel electrophoresis (page) revealed at least 10 antigens, ranging in m.w. from 35 x 103 to 160 x 103 daltons. antigens were detectable on cell su ...1977187696
cell surface antigens induced by herpes simplex virus (hsv).among the antigens induced by hsv on the surface of infected cells there are antigens binding anti-hsv antibodies by their combining (fab) site (hsv-ag), and others binding antibodies of various specificities, probably through their fc fragment (igr). hsv-ag were demonstrated by cytotoxicity and mixed agglutination tests; igr were made evident by passive hemadsorption. the ability to induce igr is especially characteristic of hsv-1 strains, being absent or limited with hsv-2 strains. igr were no ...1976188230
dna-binding proteins of cells infected by herpes simplex virus type 1 and type 2.proteins showing affinity for dna in hsv-1-and hsv-2-infected cells were compared by dna-cellulose chromatography and page. the proteins observed depended on the type of virus used to infect the cell; however, several examples of analogous polypeptides were present in cells infected by both virus types. proteins showing highest affinity for dna-cellulose were similar in molecular size in cells infected by both virus types.1976188782
correlation of herpes simplex virus types 1 and 2 with clinical features of infection.strains (338) of herpes simplex virus (hsv) were isolated in stockholm during 1965-1974. by immunoelectroosmophoresis it was possible to identify all strains as either hsv type 1 (hsv-1) or 2 (hsv-2). no strains of intermediate antigenic type or with untypable characteristics were found. the antigenic type of hsv was correlated with body site and clinical features of infection. a case of severe, recurrent, abdominal pain in association with hsv-2 infection is described. in one patient with acute ...1977188954
[properties of herpes simplex virus type 2 and its relation to cervicouterine cancer]. 1976189359
genetic and physiological studies with herpes simplex virus type 2 temperature-sensitive mutants. 1975190124
chromosome changes in hamster cell line exposed to herpes simplex virus type 2. 1975190130
quantitative transformation of swiss/3t3 cells by uv-irradiated herpes simplex virus type 2. 1975190148
transformation of mouse cells with herpes simplex virus type 2. 1975190149
transformation of embryonic rat fibroblasts by herpes simplex virus type 2. 1975190150
preliminary studies of nonvirion antigens associated with herpes simplex virus 1 and 2 (hsv1 - hsv2).biochemical and radioimmunological studies have indicated the presence of a nonvirion antigen associated with herpes virus. guinea pig kidney cells were grown in culture and infected (i-cells) for 3h with herpes virus. uninfected cells (n-cells) served as control. the cells were collected and sonicated to obtain, after centrifugation, "soluble extract" (si, sn). the pellets were extracted with kcl (isp, nsp). sn, si were submitted to gel-filtration on sephadex g-200. extracts from i and n cells ...1976191042
[investigations concerning the evidence of herpes simplex virus type 2 (hsv-2) antibodies in patients with carcinoma of the cervix (author's transl)].the neutralizing antibodies to hsv-1 and hsv-2 were determined in the sera of 128 patients. infection was detectable in nearly 100% of the cases in each of the three investigated groups (patients with carcinoma of the cervix, female patients with chronic recurrent hsv infection in the genital area and a control group without and history of hsv infection). the percentage of patients displaying hsv-2 antibodies in the group with carcinoma of the cervix (38%) is significantly higher than in the con ...1977191994
long-term follow-up studies on herpes simplex antibodies in the course of cervical cancer: patterns of neutralizing antibodies.the kinetics of neutralization was used to study antibodies to herpes simplex virus type 2 in 90 patients with invasive carcinoma of the cervix uteri. the first sera were drawn before treatment and consective sera were drawn at intervals thereafter. control groups consisted of 90 age-matched healthy women and 70 cancer patients suffering from malignancies other than cervix carcinoma. patients surviving during the observation period of 12-60 months had a significantly higher frequency of neutrali ...1977192072
immunodiagnostic potential of a virus-coded, tumor-associated antigen (ag-4) in cervical cancer.the central theme of this communication is the recognition of an immunodiagnostic potential in a herpes virus antigen, the molecular interrelationship of which with cervical tumor cells is described. in addition to the productive infection caused by herpes simplex virus type 2 (hsv-2) we are confronted by latency and, as suggested by recent studies, by cancer. these different types of virus-host cell interactions are discussed at the host, as well as at the cellular level. a defined level of mol ...1977192436
specific non-immunoglobulin g antibodies and cell-mediated response to herpes simplex virus antigens in women with cervical carcinoma.non-immunoglobulin g-neutralizing antibodies to herpes simplex virus (hsv) type 2 were assayed in sera adsorbed with staphylococcus aureus cowan i. they were present in 8% of women with normal cervical smear and in 20, 41, and 74% of women with atypia, dysplasia, and cervical carcinoma, respectively. lymphocytes of the patients were tested for in vitro transformation by killed hsv type 1 and hsv type 2 (hsv-2), as well as by mitomycin c-treated hamster cells transformed by hsv or other viruses o ...1977192446
[prevention and therapy of viral diseases with special reference to interferon].human leukocyte interferon (hli) was used for treatment of human diploid fibroblasts before and after infection with vaccinia virus, herpes simplex virus type 1 (hsv 1), herpes simplex virus type 2 (hsv 2), and varicella zoster virus (vzv). vero cells were infected with medical lake macaque herpes virus (mlmv), and treated with hli in the same way. in all of these systems hli exhibited an antiviral effect when administered before infection, and this effect could be increased by additional hli tr ...1977192653
a sensitive method for the detection of herpes simplex virus type 2 specific thymidine kinase.thymidine kinase (tk) activity of uninfected and in vitro herpes simplex virus type 2 (hsv-2) infected human fetal lung cells was analyzed. polyacrylamide gel electrophoresis was carried out, and subsequent incubation of equally divided gel slices in the presence of normal or anti-hsv-2 specific tk serum, together with different substrates, was performed. small amounts of virus-specific enzyme could be detected using this combination of electrophoresis and antibodies and replacing atp by ctp in ...1977192698
effect of immunization on acute and latent infections of vaginouterine tissue with herpes simplex virus types 1 and 2.inoculation of mice with herpes simplex virus types 1 and 2 (hsv-1 and hsv-2) by the vaginal route resulted in viral shedding during the acute phase of the infection and the development of a latent infection in vaginouterine tissue lasting up to one year in greater than 25% of the animals. immunization with either serotype of hsv markedly reduced viral shedding when animals were subsequently challenged with the homologous serotype, but immunization with hsv-2 was significantly less effective in ...1977192810
herpes simplex virus type 2 date only 9 culture-proven cases of herpes simplex virus type 2 meningitis have been recorded. presented here is the 10th case with a review of the literature. although generally a benign disease, its recognition in association with herpes progenitalis must be appreciated to avoid confusion in the treatment of this common veneral disease.1977191771
transfer of the gene for thymidine kinase to thymidine kinase-deficient human cells by purified herpes simplex viral dna.transformation of human cells from a thymidine kinase (atp:thymidine 5'-phosphotransferase, ec to a thymidine kinase-positive phenotype has been achieved by using purified dna from herpes simplex virus type 2. the specific activity of the dna was in the range 0.5 to 2.0 transformants per microng and the efficiency of gene transfer was up to 1 transformant per 10(5) recipient cells. several transformed lines able to grow continuously in medium selective for thymidine kinase-pos ...1977193108
[oncogeny of herpes simplex viruses and lupidon].the part of the herpes simplex virus type 2 in the development of the cervex carcinoma of women is still an open question. after the serological findings that patients with cervix carcinoma have a higher rate of antibodies against type 2 than healthy women, which were initially published with great expectation, the molecular-virological examinations which were meanwhile carried out by several work groups to find traces of the herpes simplex virus type 2 in the carcinoma cells were negative, exce ...1977193311
herpes simplex virus types 1 and 2 in clinical infections: differences observed by electron microscopy.microtubule-like structures have been observed ultrastructurally in bhk-21/4 cells infected with herpes simplex virus type 2 (hsv-2) but not in cells infected with herpes simplex virus type 1 (hsv-1). bhk-21/4 cells were infected with two known strains of hsv-2 and one known strain of hsv-1, as well as with vesicular fluid from 50 clinical isolates (47 perioral, two penile, and one from the leg) in an examination of the accuracy of electron microscopy for differentiation between clinical isolate ...1977191545
multicystic cerebral degeneration in neonatal herpes simplex virus encephalitis.typical herpetic papulovesicular skin lesions developed in an apparently normal infant at 12 days of age and were followed within 48 hours by signs and symptoms of acute encephalitis. herpes simplex virus type 2 was cultured from the intact skin vesicles, and a fourfold increase in complement fixation titer to herpes simplex virus type 2 was found over the ensuing 24 days. the infant survived her acute illness, but was left with severe neurologic sequelae manifested as microcephaly with multicys ...1977193394
cell-mediated immune responses in recurrent herpesvirus infections. i. lymphocyte proliferation assay.studies in immunosuppressed and immunodeficient patients indicate that the cell-mediated immune response appears to be responsible for controlling reactivated herpesvirus infections. in this study, the various parameters of a herpesvirus (types 1 and 2) antigen specific lymphocyte proliferation assay were optimized and used to evaluate individuals with clinical, recurrent hsv-1 and hsv-2 infections. normal individuals with neutralizing antibody to hsv-1 or hsv-2 responded to virus antigen in cul ...1977191528
biochemical and immunological characterization of deoxythymidine kinase of thymidine kinaseless hela cells biochemically transformed by herpes simplex virus type.thymidine kinase (tk) from herpes simplex virus type 1 (hsv-1) biochemically transformed hela cells, purified by affinity chromatography, has been characterized with respect to its electrophoretic mobility, molecular weight, activation energy, substrate specificity, and immunological specificity. tk purified from hsv-1-transformed hela cells has the same electrophoretic mobility as tk purified from hela cells lytically infected with hsv-1. the sedimentation velocity of purified tk from transform ...1977193790
efficacy of herpes simplex virus type 1 immunization in protecting against acute and latent infection by herpes simplex virus type 2 in mice.icr mice were immunized with herpes simplex virus type 1 (hsv-1) and later challenged with hsv-2 by footpad inoculation. both immunized animals and age-matched, nonimmunized controls were observed for ascending neurological disease and latent infection of spinal ganglia resulting from the hsv-2 challenge. control animals had a 78% incidence of acute and latent infection compared with a 1.7% incidence in immunized mice. the data show immunity to hsv-1 is protective against both acute and latent i ...1977193798
effect of hormones on herpes simplex virus type-2-induced transformation. 1977194162
comparison of dna facilitators in the uptake and intracellular fate of infectious herpes simplex virus type 2 dna.the comparative efficiencies of polyornithine, cacl2 and deae-dextran in enhancing the infectivity of exogenous herpes simplex virus (hsv) type 2 dna were examined. cacl2 was 12-times more effective in promoting genetic expression of viral dna than deae-dextran, while polyornithine did not mediate any hsv dna infectivity. a comparison of sedimentation profiles of dna extracted from cells inoculated with viral dna in the presence of each facilitator revealed that input 56 s hsv dna underwent mark ...1976187236
purification of herpesvirus nucleocapsids by fluorocarbon extraction.purified nucleocapsids were prepared from herpes simplex virus type-1 and herpes simplex virus type-2 infected cells, using a fluorocarbon extraction technique with freon 113. fifty to 85% of the total nucleocapsid population remained 'nucleated' or full in terms of the nucleic acid core making them useful as a source of dna. cscl banded dna represented 30--40% of the theoretical yield of the total nucleocapsid population.1976195183
quantification of the herpes simplex virus dna present in biochemically transformed mouse cells and their revertants.four cell lines biochemically transformed by u.v.-irradiated herpes simplex virus contain virus dna fragments ranging from 3 to 22% of the hsv genome. of five revertant clones selected for 3h-tdr or brdurd resistance, four had lost all detectable virus dna while the fifth, selected for brdurd resistance, retained the entire virus fragment but there was a reduction of virus copies per cell from 5 to 1. three 'supertransformed' revertant cell lines contained virus dna fragments ranging from 12 to ...1977196037
ultrastructural effects of herpes simplex virus type 2 infection of rat dorsal root ganglia in culture.the ultrastructural consequences of herpes simplex virus type 2 (strain r-2) infection of organotypic cultures of embryonic rat dorsal root ganglia were studied. the intial consequences (dilation of endoplasmic reticulum and/or golgi apparatus and distortion of mitochondrial structure) occurred in the cytoplasm. these effects were followed by several nuclear changes including loss of nucleoplasm, and margination of chromatin. extensive nuclear membrane proliferation was accompanied by the viral ...1977196052
type specificity and use of human sera for rapid typing of herpes simplex virus isolates by an indirect peroxidase-labeled antibody technique: a comparison with three other methods.human sera from patients showing seroconversion following a primary herpes simplex virus type 1 (hsv-1) or hsv type 2 (hsv-2) infection, as well as 19s and 7s fractions obtained from the same sera, have been used for typing 191 hsv isolates by an indirect peroxidase-labeled antibody (pla) method. typing has also been performed on all of the isolates using a microneutralization (mn) and the indirect hemagglutination (iha) inhibition test. furthermore, 30 isolates have been typed by a plaque metho ...1977196572
replication of herpes simplex virus in mouse spleen cell cultures stimulated by lipopolysaccharide.replication of hsv was demonstrated in spleen cell cultures of d2 and several other strains of mice after prestimulation with mitogenic doses of lps for 2 days. no viral replication occurred in unstimulated cultures or in cultures prestimulated with pha and con a, whereas there was some viral replication in spleen cell cultures of d2 mice after prestimulation with poly i-c. spleen cells of b6 mice did not support replication of hsv under any of the conditions we have tested thus far. the reasons ...1976186534
immunopotentiation and antiviral chemotherapy in a suckling rat model of herpesvirus encephalitis.encephalitis was induced in 10-day-old wistar rats by intraperitoneal injection of approximately 100 50% tissue culture infective doses of herpes simplex virus type 2. treatment regimens included immunopotentiation with levamisole and combined therapy with levamisole and an antiviral agent, adenine arabinoside. rats treated with levamisole alone had significantly higher rates of survival than placebo-treated controls 14 days after injection of virus. combination therapy with levamisole and adeni ...1976180203
chromosomal site(s) of integration of herpes simplex virus type 2 thymidine kinase gene in biochemically transformed human cells. 1977197027
laser cytophotometric detection of variations in spatial distribution of concanavalin-a cell surface receptors following viral infection.human cells in culture (h.ep. 2) were infected with herpes simplex virus type 2 at a multiplicity of infection (m.o.i.) of 3 for 3, 18 and 36 hr. spatial redistribution of concanavalin (con-a) cell surface receptor sites was detected by an indirect double-antibody (peroxidase-conjugated goat anti-rabbit to rabbit anti-con-a) immunoenzymatic staining reaction which rendered the con-a sites visible to light microscopy and to flow laser cytophotometry. significant spatial redistribution of cell sur ...1977197163
the photoinactivation of an rna animal virus, vesicular stomatitis virus, with the aid of newly synthesized psoralen derivatives.the newly synthesized psoralen derivatives, 4' hydroxymethyl 4,5',8 trimethylpsoralen, 4' methoxymethyl 4,5',8 trimethylpsoralen, and 4' aminomethyl 4,5',8 trimethylpsoralen hydrochloride photoreact with the single-stranded rna animal virus, vesicular stomatitis virus, vsv. this virus is inactivated 10(3) times more effectively by photoreaction with these compounds than when photoreacted with 4,5',8 trimethylpsoralen. under these conditions the rna virus remains more than 10(3) times less sensit ...1977197489
antiviral activity of arabinosylthymine in herpesviral replication: mechanism of action in vivo and in vitro.1-beta-d-arabinofuranosylthymine (ara-t), a metabolite of the sponge tethya crypta, has shown selective activity against herpes simplex virus (hsv) replication (g. a. gentry and j. f. aswell, virology 65:294-296, 1975). analysis of hsv-infected and uninfected cell lysates by cscl isopycnic centrifugation showed that ara-t blocked the incorporation of [(3)h]hypoxanthine into viral deoxyribonucleic acid and, to a large extent, into host deoxyribonucleic acid of infected (but not uninfected) cells. ...1977197886
anatomy of herpes simplex virus dna. ix. apparent exclusion of some parental dna arrangements in the generation of intertypic (hsv-1 x hsv-2) recombinants.we are reporting the physical location of parental dna sequences in 28 recombinants produced by crossing herpes simplex viruses (hsv) 1 and 2. the parental crosses were of two kinds. in the first, temperature-sensitive mutants of hsv-1 and hsv-2 were crossed to produce wild-type recombinants. in the second, temperature-sensitive mutants of hsv-1 rendered resistant to phosphonoacetic acid were crossed with wild-type hsv-2, and recombinants that multiplied at nonpermissive temperature and were res ...1977198577
prolongation of herpes simplex virus latency in cultured human cells by temperature elevation.treatment of herpes simplex virus type 2 (hsv-2)-infected human fibroblast cells with cytosine arabinoside (ara-c) at 25 microgram/ml resulted in complete inhibition of virus replication. removal of ara-c after 7 days of treatment ultimately resulted in renewed virus replication, but after a delay of at least 5 days. if however, the temperature was elevated from 37 degrees c to 39.5 to 40 degrees c at the time of ara-c reversal, infectious hsv-2 did not reappear. as long as the cultures were mai ...1977198583
the detection of virus dna sequences in a herpes type 2 transformed hamster cell line (333-8-9).herpes simplex virus type 2 dna labelled in vitro with 125i has been used as a probe to search for virus dna sequences in the 333-8-9 line of transformed primary hamster cells and in clines derived from the line. virus dna sequences were present when the cells were examined initially but these sequences were lost on subsequent handling. no virus sequences were detected in clones derived from the line. it is suggested that the presence of virus dna is an unstable characteristic of the cell line a ...1976179957
complement-dependent cytotoxic antibodies in the course of cervical carcinoma.complement-dependent cytotoxic antibodies to three cervical carcinoma cell lines (me-180, sw-732 and hela) and to herpes simplex virus type 2 (hsv-2)-infected cells were determined in a long-term study of women with cervical carcinoma. cytolysis of surface antigens differed significantly between the cervical carcinoma cell lines and hsv-2. regression of the tumor during treatment was accompanied by decreasing cytolysis in the cervical carcinoma lines, while tumor bearers and patients who became ...1977200570
herpes simplex virus dna in transformed cells: sequence complexity in five hamster cell lines and one derived hamster tumor.analyses of the hybridization kinetics of labeled herpes simplex virus 2 (hsv-2) dna with dna from five hamster cell lines transformed by uv light-irradiated hsv-2 revealed the following. (i) viral dna sequences were detected in all five cell lines tested. (ii) none of the cell lines contained the full complement of hsv-2 dna. (iii) the amount of viral dna present in the cells varied in different transformed cell lines and ranged from 8 to 32% of the hsv-2 dna genome in 1 to 3 copies/cell. (iv) ...1976178927
[herpes simplex virus (hsv-1 and hsv-2) infection: its clinical and oncogenic properties (author's transl)].the epidemiology and clinical features of diseases caused by the herpes simplex virus, are reviewed and recent results are discussed which give an insight into the complex mechanism of primary and chronic, recurrent hsv-1 infections. immunological reactions in hsv infection and data concerning the oncogenic potential of hsv-1 and -2 are dealt with. furthermore, current therapeutic possibilities are outlined.1977201107
studies on tumors produced by cells transformed with herpes simplex virus type 2.biological, histological, and ultrastructural studies were made on tumors produced in weanling hamsters inoculated with hamster embryo fibroblasts (hef) transformed by herpes simplex virus type 2 or with cultured cells (tumor cell lines t1 and t2) derived from the tumor tissues. the malignancy of transformants increased through passages in vitro and in vivo. histologically, two tumor cells lines (155-4t1 and u-15t1) produced fibrosarcomas and one (u-26t1) produced lesions resembling "malignant f ...1977201537
an investigation into the relationship of the immunological status of herpes simplex virus type-2 (hsv-2) infected animals and the tumor-enhancing property of this order to compare the state of immunity of hsv-2 infected animals with their sham treated counterparts, several in vivo methods for cell-mediated immunity in addition to the stimulation in vitro of mouse splenocytes with pha were performed. the grafting of c 3 h mice with heterologous skin and the paw test with pha showed no noticeable difference between the two groups. the tuberculin test performed on guinea pigs infected via the footpads, permitted the detection of a slightly impaired reacti ...1977201898
prospective double-blind evaluation of topical adenine arabinoside in male herpes progenitalis.thirty-four virologically proven episodes of herpes progenitalis in 32 men were treated in a prospective double-blind study with either adenine arabinoside ointment or an identical-appearing placebo for 7 days. clinical evaluation and quantitative virological studies were done on days 1, 3, and 8. there was a highly significant correlation between clinical response and quantitative virology. there was no difference in clinical or virological response between drug and control groups. primary atta ...1975174489
production of group- and type-specific antigens during non-permissive infection of dog kidney cells with herpes simplex virus type 2. 1978203280
macrophages and age-dependent resistance to hepatitis induced by herpes simplex virus type 2 im age-dependent increase in the resistance of balb/c mice to induction of focal necrotic hepatitis by herpes simplex virus type 2 was demonstrated. in 3-week-old mice inoculated intraperitoneally with virus, numerous necrotic foci developed in the liver. as the mice matured, the number of lesions declined until the age of 8 weeks, when no further increase in resistance appeared. corresponding to this, the virus titers of livers and spleens of 3-week-old mice were higher than in 8-week-old anima ...1978203536
radiometric detection of herpes simplex viruses.two radiometric techniques were investigated as possible means of detecting viruses in clinical specimens. the effect of herpes simplex virus on dna synthesis by monolayers of human embryonic lung fibroblasts was monitored by the incorporation of [3h] thymidine or [125i] iododeoxyuridine ([125i] idu). radiometric results were compared with those obtained from visual examination for cytopathic effects in the same cell line. cells infected with herpes simplex virus type-2 (hsv-2, 10(6.8) virions) ...1978203666
[photodynamic inactivation of herpes simplex virus: ii. photodynamic inactivation with proflavine in experimental herpetic keratitis (author's transl)]. 1977204176
herpes simplex virus type 2 induced pyrimidine nucleoside kinase: enzymatic basis for the selective antiherpetic effect of 5-halogenated analogues of deoxycytidine. 1978204334
the epidemiology and interrelationship of cervical dysplasia and type 2 herpesvirus in a low-income housing project.this report examines the association between cervical dysplasia and herpes simplex virus type 2 antibodies in a low-income housing project, atlanta, georgia. we established a clinic in this project at which women could receive a breast examination and papanicolaou smear and have blood drawn to be tested for herpesvirus type 2 antibodies. prevalence of herpesvirus rose sharply as age increased. there was a significant absence of antibodies in young, nulliparous women. dysplasia showed a tendency ...1975170825
incidence and distribution of herpes simplex virus types 1 and 2 from genital lesions in college women.during a 9-month period, 9,772 women were treated at the student health center's gynecology clinic. herpes simplex virus was isolated from 30 of 57 patients clinically diagnosed as suffering from a herpetic or herpetic-like genial infection for a virological incidence rate of 0.31%. using virus plaque diameter in chick embryo cells and heat stability of viral thymidine kinase, 37% of the isolates were classified as herpes simplex virus type 1 and 63% were classified as herpes simplex virus type ...1977204741
chromosome abberations in rat embryo cells transformed after infection with ultraviolet-irradiated herpes simplex virus.two rat embryo cell lines (tr1 and tr2) transformed after infection with ultaviolet-inactivated herpes simplex virus type 2 showed modal chromosome numbers in the tetraploid range. in the tr1 cell line a near-diploid cell population replaced during subcultivation the hypotetraploid cells thus explaining the loss of immunofluorescent viral antigens. both transformed cell lines showed frequent achromatic gaps. in the tr1 cell line such gaps were preferentially located in specific chromosome region ...1978205041
induction of human cell dna synthesis by herpes simplex virus type 2.the effect of herpes simplex virus type 2 (hsv-2) infection on the synthesis of dna in human embryonic fibroblast cells was determined at temperatures permissive (37 c) and nonpermissive (42 c) for virus multiplication. during incubation of hsv-2 infected cultures at 42 c for 2 to 4 days or after shift-down from 42 to 37 c, incorporation of (3h)tdr into total dna was increased 2-to 30-fold as compared with mock-infected cultures. analysis of the (3h)dna suggested that host cell dna synthesis was ...1975163370
production of plasminogen activator by cells transformed by herpesviruses.plasminogen activator is produced by hamster cells transformed by human herpesviruses. these cell lines have previously been shown to be oncogenic when injected s.c. into newborn syngeneic hamsters. lysis of fibrin overlays by these cell lines was plasminogen dependent. normal hamster embryo fibroblasts and a hamster cell line transformed by para-7 (an adenovirus-sv 40 hybrid) failed to produced lysis. in separate experiments fibrin overlay of lytically infected secondary rabbit kidney cells did ...1978205345
role of activated macrophages in resistance of congenitally athymic nude mice to hepatitis induced by herpes simplex virus type 2.congenitally athymic nude (nu/nu) mice of a balb/c genetic background were found considerably more resistant to the induction of focal necrotic hepatitis by herpes simplex virus type 2 (hsv-2) tha, were phenotypically normal littermates (nu/+) or balb/c mice. the augmented resistance was age dependent, as it was only manifested in mice from 4 to 5 weeks of age. studies of the course of infection showed that nude mice were able to restrain virus multiplication in the liver far better than normal ...1978205507
quantitative assay for transformation of 3t3 cells by herpes simplex virus type 2.the interaction of herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) with swiss/3t3 cells was investigated. virus-induced cytopathic effects developed in the absence of production of infectious virus. hsv-2 inactivated with uv light (2, 4, 6, and 8 min) also induced cell death in the absence of virus replication. cell death was not detectable after infection by hsv-2 that had been inactivated by uv irradiation for 10, 12, and 14 min. 3t3 cells infected with uv-inactivated virus (10 and 12 m ...1975163369
biochemical transformation of mouse cells by herpes simplex virus type 2: enhancement by means of low-level photodynamic treatment.the biochemical transformation of thymidine kinase-deficient cells by uv-inactivated herpes simplex virus is enhanced by low-level photodynamic treatment of the infected cells. at the concentration of proflavine used, the virus was not inactivated and both virus and cellular dna syntheses were only marginally inhibited. the observed enhancement of the transfer of a virus gene to the cell genome suggests a possible cocarcinogenic role for photodynamically active dyes at very low concentrations.1978206727
congenital herpes simplex virus, type 2, bilateral endophthalmitis.a set of dizygotic twins, both born prematurely, developed herpes simplex encephalitis shortly after birth. the second twin had unilateral keratoconjunctivitis and bilateral endophthalmitis and subsequently died from disseminated herpes infection. herpes simplex, type 2, was isolated from conjunctiva of both eyes, cerebrospinal fluid, nasopharynx and trachea. histologic examination of the eyes reveaed bilateral endophthalmitis with a necrotizing retinitis. intranuclear inclusion bodies were demo ...1977206999
resistance to vaginal or systemic infection with herpes simplex virus type 2. 1978207239
alkaline dnase activity in cells infected with a temperature-sensitive mutant of herpes simplex virus type 2.bhk cells infected with the temperature-sensitive mutant ts13 of herpes simplex virus type 2 at a nonpermissive temperature lack the alkaline nuclease activity, which is induced by the mutant at a permissive temperature and by wild-type virus at either temperature. for ts13, enzyme activity could be induced by a temperature shift to permissive conditions, but not in the presence of cycloheximide. after a shift from permissive to nonpermissive conditions in the presence of cycloheximide, the acti ...1978207883
identification of a common antigen of herpes simplex virus bovine herpes mammillitis virus, and b immunoelectrophoretic analyses one common antigen was demonstrated in antigen preparations from herpes simplex virus types 1- and 2- (hsv-1 and hsv-2), bovine herpes mammillitis (bhm) virus-, and b virus-infected cells solubilized by triton x-100. the antigen was also demonstrated in solubilized purified hsv-1 and bhm virus. the common antigen was identified as antigen 11 of hsv-1 or hsv-2. differences were found in the polypeptide composition of the related antigens when isolated from the fo ...197878988
5,6-dihydro-5-azathymidine: in vitro antiviral properties against human herpesviruses.5,6-dihydro-5-azathymidine (dhadt), a novel water-soluble nucleoside antibiotic, inhibits herpes simplex virus type 1 (hsv-1) in appropriately infected cell cultures to a greater extent than herpes simplex virus type 2 (hsv-2). vaccinia virus was less susceptible than hsv-2, and pseudorabies virus yields were not reduced at the concentrations studied. plaque formation by varicella-zoster virus was suppressed by dhadt. dhadt was slightly toxic to cells at concentrations that were inhibitory for h ...1978208459
protective effect of an oral infection with herpes simplex virus type 1 against subsequent genital infection with herpes simplex virus type 2.the problem of whether oral herpes simplex virus type 1 (hsv-1) infection provides protection against subsequent genital infection by herpes simplex virus type 2 (hsv-2) was investigated. mice were used as models. following conditions in man, both the oral and genital infections applied were noninjurious. mice infected orally with hsv-1 were weakly protected against virus 'take' following vaginal challenge with hsv-2. genital 'takes' were found in 67% of the immunized mice, as compared with 83% ...1978209295
[herpes simplex virus type 2 and cervix cancer]. 1978209356
conditions required for induction of murine p30 by herpes simplex virus.mouse cells (line n cia cl10) contain 1.2-2.5 ng murine leukaemia virus (mulv) p30 antigen/mg of protein; this amount of antigen is measurable by competition radioimmunoassay (ria) but is not detectable by indirect immunofluorescence (if). infection of n cia cl10 cells with herpes simplex virus type 2 (hsv-2) induces expression of mulv p30. induction by hsv-2 does not require either cell or virus dna synthesis and is optimal 8 h post infection when cells at 50-70% confluence are infected at a mu ...1978210128
serum antibodies to herpesvirus early antigens in patients with cervical carcinoma determined by anticomplement immunofluorescence technique.sera from patients with carcinoma of the uterine cervix were used with the anticomplement immunofluorescence (acif) technique to develop a simple procedure for detection of antibodies to herpes simplex virus type 2 (hsv-2) early antigens. test cells used in the acif assay were hep-2 cells infected with hsv-2 sequentially treated with inhibitors of protein and of dna synthesis. the cells were first treated for 7 h with cycloheximide (100 mug/ml) and then for 3 h with hydroxyurea (150 mug/ml). in ...197662718
herpes simplex virus type 2 and cancer of the prostate.a seroepidemiologic study was performed to determine if there was an association between antibodies to herpes simplex virus type 2 and cancer of the prostate, similar to that reported for herpes simplex virus type 2 and cervical carcinoma. no significant difference in herpes simplex virus type 2 antibodies was seen between patients with cancer of the prostate and those with benign prostatic hypertrophy. further studies are needed to define the possible role of herpes simplex virus type 2 in the ...197662063
herpes simplex virus type 2 infection of neonatal mice.we have demonstrated that newborn mice can be infected orally with herpes simplex virus type 2. transplacental and transvaginal transmission of herpes simplex virus type 2 did not occur in our mice. failure to produce natural transvaginal infection in mice may be explained by the relatively short period of exposure to the virus during parturition. these experiments demonstrate that high oral doses of herpes simplex virus type 2 can produce disseminate herpes neonatal infection in mice and theref ...1978211091
in vivo and in vitro "markers" of human cervical intraepithelial neoplasia.the human uterine cervix offers a unique opportunity to study the early lesions of squamous cell carcinoma, i.e., carcinoma in situ and dysplasia [combined as cervical intraepithelial neoplasia (cin)]. in vivo, the patients with cin have the epidemiological common denominators or "markers" of early onset of coitus, multiple sexual partners, 1st delivery before age 20, and antibodies to herpes simplex virus type 2 more frequently than do controls. the lesions themselves have specific epithelial a ...197658720
complement-mediated cytolysis of hsv-1 and hsv-2 infected cells: plasma membrane antigens reactive with type-specific and cross-reactive antibody.surface antigens of bhk-21 cells infected with hsv-1 or hsv-2 were radioiodinated (125i) with lactoperoxidase, immune precipitated and analysed by polyacrylamide gel electrophoresis (page). experiments using antiserum to hsv-1 or hsv-2, absorbed with appropriate hemotypic or heterotypic antigens, revealed that both type-specific (homotypic) and cross-reactive antibody combined with surface glycoproteins to form a single large radioactive peak. this peak, which constituted the major glycoprotein ...1978211189
herpes simplex virus necleic acid synthesis following infection of non-permissive xc cells.dna hybridization kinetic analysis of cellular dna following high multiplicity infection of non-permissive xc cells by herpes simplex virus type i showed that hsv dna penetrates to the nucleus of the cell but that the number of virus dna copies present in each cell quickly begins to decline. there did not appear to be any net virus dna synthesis and the loss of virus dna copies continued until there was approximately one per haploid genome equivalent. hsv-2 likewise did not show any detectable v ...1978211197
serological and epidemiological considerations of the role of herpes simplex virus type 2 in cervical assess the possible biological significance of the observations that women with cervical cancer tend to be younger at first intercourse than control women, data from 1823 women were analyzed for the relationship between age at 1st intercourse and number of sex partners. women who were younger at first intercourse had more sex partners than did women who were older at first intercourse. the interdependence of age at first intercourse and number of sex partners does not exlude the possibility t ...197656227
evidence for a herpes simplex virus-specific factor controlling the transcription of deoxypyrimidine kinase.a cistron-specific, enzyme-forming-capacity method was used to study the control of herpes simplex virus (hsv)-specific deoxypyrimidine kinase (dpyk) mrna synthesis. a virus-specific factor was formed by a primary infecting virus, and this factor effected the transcription of dpyk mrna of a superinfecting virus in the presence of cycloheximide, suggesting that the factor acted in "trans" and was a diffusible one. after the addition of actinomycin d to prevent further transcription and upon remov ...1978211259
cellular immune response in genital herpes simplex virus infection.we studied the relations between the cellular immune response, pre-existing complement-fixing antibody and virus type with duration of virus excretion in genital herpes simplex virus (hsv) infection. thirty-six patients (seven with hsv-1 and 29 with hsv-2) with genital herpes underwent serologic testing, sequential viral cultures and weekly determination of lymphocyte-transformation stimulation index with inactivated hsv antic n. the duration of virus excretion was shortest in those with pre-exi ...1978211414
differential feulgen-deoxyribonucleic acid hydrolysis patterns of herpes simplex virus type 1 and type 2 infected cells.infection of human embryonic lung cells with herpes simplex virus type 1 (hsv-1) and herpes simplex type 1 (hsv-2) resulted in: (a) qualitative (nuclear cytopathologic) alterations and quantitative (nuclear area) differences in infected compared to control nuclei; (b) increased feulgen-deoxyribonucleic acid (f-dna) amounts in infected cells, probably due to viral dna; (c) higher f-dna levels in hsv-2 infected cells; and (d) increased rates of f-dna hydrolysis in viral-infected as compared to uni ...197547871
optical diffraction analysis of electron microscope images of tubular structures found in cells infected with herpes simplex virus type 2.optical diffraction analysis was done on the electron micrographs of tubular structures found in cells infected with herpes simplex virus type 2. by this method, the helical arrangement of subunits forming a tubular structure was demonstrated.1978212515
ribonucleotide reductase from herpes simplex virus (types 1 and 2) infected and uninfected kb cells: properties of the partially purified enzymes.mammalian ribonucleotide reductase is a complex enzyme modified in its activity by a complex regulatory system involving adenosine triphosphate (atp) and deoxyribonucleoside triphosphates. infection of kb cells with herpes simplex virus (hsv) type 1 or 2 induces the formation of an altered ribonucleotide reductase. the properties of partially purified reductase from uninfected kb cells have been compared with the enzymes obtained from hsv-1 and hsv-2 infected kb cells. we found that the virus-in ...197718550
collaborative complementation study of temperature-sensitive mutants of herpes simplex virus types 1 and 2.twenty-three complementation groups of herpes simplex virus type 1 (hsv-1) and 20 of hsv-2 were identified by qualitative and quantitative complementation analysis from among 43 temperature-sensitive (ts) mutants of hsv-1 and 29 ts mutants of hsv-2 which had been isolated independently in 10 laboratories.1978212578
a rational approach to the development of antiviral chemotherapy: alternative substrates of herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) thymidine kinase (tk). 1977212988
antibodies to herpes simplex virus types 1 and 2 in patients with squamous-cell carcinoma of uterine cervix in india.antibody activity to herpes simplex virus type-1 (hsv-1) and type-2 (hsv-2) was measured by the indirect hemagglutination (iha) test in sera from 124 women with squamous-cell carcinoma of the uterine cervix, 46 women with non-cervical cancer and 116 matched normal women. the type-specific antibodies were assayed by iha inhibition test. the proportion of cervical cancer cases positive for hsv-2 infection was significantly greater than that of non-cervical cancer cases and matched control women. n ...1978214407
recombinants between herpes simplex virus types 1 and 2: analyses of genome structures and expression of immediate early polypeptides.recombinants between temperature-sensitive mutants of herpes simplex virus types 1 (hsv-1) and 2 (hsv-2) were constructed. using restriction endonucleases, we analyzed the genome composition of 17 intertypic recombinants and detected crossovers in every region of the genome. the virion dna of one recombinant appeared to be largely "frozen" in two of the four possible genome arrangements of hsv. knowledge of the genome structures of recombinants enabled us to physically map immediate early polype ...1978214575
colposcopic examination of the cervix of cebus monkeys.a total of 410 colposcopic examinations were performed on 188 female cebus monkeys that were under study to determine the oncogenic potential of herpes simplex virus type 2 in this genus. a split-cone vaginal speculum was developed that permitted good observation of the vaginal cervix in the cebus monkey. the cervical anatomy of cebus monkeys was found to differ from that of humans in that the surface of the animal cervix was more papilliform, with thinner squamous epithelium, and the squamocolu ...1978214730
effect of treatment with 5-ethyl-2'-deoxyuridine on herpes simplex virus encephalitis in normal and immunosuppressed mice.5-ethyl-2'-deoxyuridine (5-ethyl-durd), an analog of thymidine, was evaluated for its capacity to inhibit herpes simplex virus (hsv) replication in vitro and in vivo. the 50% effective dose concentration of 5-ethyl-durd for hsv types 1 and 2 (hsv-1 and -2) cultured in vero cells was 6 and 9 mug/ml, respectively. levels of 5-ethyl-durd 14-fold in excess of the 50% effective dose for hsv-1 did not inhibit the formation of confluent monolayers by vero cells, suggesting that the compound was not cyt ...1978215081
the tumor enhancing property of herpes simplex virus type-2 (hsv-2).the s.c. infection of dbf-1 mice with hsv-2 has a tumor enhancing effect on simultaneously i.m. implanted mca-induced syngeneic spindle cell sarcoma. thus with the dosage used here, the first palpable tumors appeared on day 6 in the virus treated group as compared to day 9 p.i. in the sham treated batch. further, more tumors were formed: 75% among the infected and 40% among the sham injected. the tumor yield could be modulated: no difference between the 2 groups was found when the tumor cell dos ...1977193079
comparison of various macrophage-inhibitory agents on vaginal and systemic herpes simplex virus type 2 infections.pretreatment of mice intraperitoneally with silica, trypan blue, or dextran sulfate to inhibit macrophage function markedly increased the lethality of a systemic intravenous infection with herpes simplex virus type 2, but did not affect the lethality or local virus growth after vaginal infection of mice with herpes simplex virus type 2. agents which inhibit macrophage function by different mechanisms decreased host resistance to herpes simplex virus type 2, but the effects of macrophage-inhibito ...1978215548
characterization of human tk- cell lines transformed to a tk+ phenotype by herpes simplex virus type 2 dna.human tk- cells carrying the hsv-2 tk gene as a result of transformation with virus dna express a tk activity of virus origin and maintain the tk+ phenotype when grown in hat medium. under non-selective conditions, however, reversion to a tk- phenotype occurs with a significant frequency characteristic of each transformed line. once reversion has occurred the tk- phenotype appears to be stable, since only very rare instances of tk- to tk+ reversion have been observed. tk- revertants were suscept ...1979215706
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