Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| smoking and infectious agents and risk of in situ cervical cancer in sydney, australia. | in a study of 116 in situ cervical cancer patients and 193 matched community controls in sydney, australia, smoking was found to be a major risk factor. current smokers had a adjusted relative risk [rr] of 4.5 compared to nonsmokers [95% confidence interval (ci) 2.2-9.1] and exsmokers a rr of 1.3 [95% ci 0.6-3.0]. there was a stepwise dose-response relationship between risk and number of cigarettes smoked (30+ cigarettes/day, rr = 5.1, 95% ci 1.5-17.3); this dose-response relationship was more m ... | 1989 | 2547521 |
| efficacy of oxetanocin g against herpes simplex virus type 2 and murine cytomegalovirus infections in mice. | 1989 | 2547744 | |
| replacement of the pseudorabies virus glycoprotein giii gene with its postulated homolog, the glycoprotein gc gene of herpes simplex virus type 1. | giii, the major envelope glycoprotein of pseudorabies virus (prv), shares approximately 20% amino acid similarity with glycoprotein gc of herpes simplex virus type 1 (hsv-1) and hsv-2. we describe here our first experiments on the potential conservation of function between these two genes and gene products. we constructed prv recombinants in which the giii gene and regulatory sequences have been replaced with the entire hsv-1 gc gene and its regulatory sequences. the gc promoter functions in the ... | 1989 | 2548015 |
| enhancement of herpes simplex virus type 2 (hsv-2) dna synthesis in infected cells that constitutively express the bglii-n region of the hsv-2 genome. | the bglii-n fragment of the herpes simplex virus type-2 (hsv-2) genome encodes one of two known transforming regions of this dna virus. in this study, we report the derivation of hela s3 cells (2dc4) that stably express the hsv-2 bglii-n region, including the small subunit of hsv-2 ribonucleotide reductase (rr). superinfection of the 2dc4 cells with wild-type hsv-2 resulted in the efficient induction of hsv-2-encoded icp10, dna polymerase, and thymidine kinase. the amount of hsv-2 dna synthesis ... | 1989 | 2548338 |
| a herpes simplex virus type 2 variant in which a deletion across the l-s junction is replaced by single or multiple reiterations of extraneous dna. | we have isolated and characterized a novel variant of herpes simplex virus type 2 (hsv-2) strain hg52 which has a deletion of 13.5 kb across the l-s junction of the genome, resulting in all of the irl region, half of the irs region and the intervening l-s junction 'a' sequence(s) being removed. the deleted dna has been replaced by single or multiple (up to 14) reiterations of a dna sequence approximately 1 kb in length. individual genomes within the population range in size from approximately 12 ... | 1989 | 2549183 |
| retention and expression of the left end subfragment of the herpes simplex virus type 2 bglii n dna fragment do not correlate with tumorigenic conversion of nih 3t3 cells. | cotransfection experiments have been carried out using recombinant plasmids pag60, conferring resistance to antibiotic g418, and pxho3 which contains the left end subfragment (map coordinates 0.583 to 0.596) of the transforming herpes simplex virus type 2 bglii n dna fragment and encodes the 36k polypeptide associated with the viral ribonucleotide reductase activity. several nih 3t3 cell clones resistant to g418 and having morphological changes commonly observed for transformed nih 3t3 cells wer ... | 1989 | 2549186 |
| [detection of igg and igm antibodies to herpes simplex virus type 1 and type 2 in human serum using an immunoenzyme method (elisa) and the complement fixation reaction]. | on the basis of borderline optical density values (od) in an enzyme-linked immunosorbent assay (elisa) resulting from comparative testing of this method with complement fixation reactions (cf), determination was made of a set criteria for negative, weakly positive and positive results of igg and igm antibody detection of herpes simplex type 1 (hsv 1), respectively type 2 (hsv 2). of the 192 examined specimens by elisa, 69.2% and 49.5% igg hsv 1 and hsv 2 antibodies were found respectively. the n ... | 1989 | 2549311 |
| herpes simplex virus type 2 antibodies: high prevalence in monogamous women in costa rica. | we studied the prevalence of antibody to herpes simplex virus types 1 and 2 (hsv-1 and hsv-2) in 766 randomly selected costa rican women 25-59 years of age in a national household survey in 1984-1985. overall, 97.1% were seropositive for hsv-1 and 39.4% for hsv-2. only 1.1% of hsv-2 seropositive women gave a history of symptomatic genital herpes. hsv-2 virus antibody increased with age and with the number of lifetime sexual partners. hsv-2 seroprevalence among women who reported only 1 lifetime ... | 1989 | 2549796 |
| viral etiology of cervical carcinoma. human papilloma virus and herpes simplex virus type 2. | the possible role of human papilloma virus (hpv) and herpes simplex virus type 2 (hsv-2) in the viral etiology of cervical carcinoma was investigated a series of cervical lesions were studied for the presence of hpv and hsv-2 dna sequences as well as hpv and hsv-2 antigens by dna dot blot hybridization technique and high-specificity pap staining method. the results obtained were correlated with the histologic diagnosis. hpv 16 dna sequences detected in cervical carcinoma biopsies were 43%, where ... | 1989 | 2550184 |
| clinical and serological outcome of genital herpes simplex virus (hsv) type 2 inoculation following oral hsv type 1 infection in guinea-pigs. | the clinical and serological outcome of genital herpes simplex virus type 2 (hsv-2) inoculation in animals previously orally infected with hsv type 1 was evaluated. a prior hsv-1 oral infection modified the genital hsv-2 infection so that only four of 18 (22%) animals were initially symptomatic although all but one animal shed hsv-2 from the cervicovaginal area for at least 5 days following inoculation. three of four animals with symptomatic initial disease also developed recurrences, as did an ... | 1989 | 2550572 |
| antigen-presenting capacity of epidermal cells infected with vaccinia virus recombinants containing the herpes simplex virus glycoprotein d, and protective immunity. | we studied the association of herpes simplex type 1 (hsv-1) glycoprotein d (gd-1) expression in epidermal cells (ec) with virus-specific immunity and protection of mice from fatal hsv-2 challenge. vaccinia virus recombinants containing gd-1 under the control of an early (vp176) or late (vp254) vaccinia virus promoter were used. mature gd-1 protein was expressed in vp176-infected ec and they had accessory cell function for hsv-2-induced t cell proliferation of immune lymph node cells (lnc). it wa ... | 1989 | 2550579 |
| evaluation of a new herpes simplex virus typing reagent for tissue culture confirmation. | monoclonal antibody typing reagents for herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) from california integrated diagnostics, inc. (berkeley, ca) were compared to two other commercially available hsv monoclonal antibody typing reagents. of the 105 specimens tested, of which 81 were positive for hsv, there was 100% agreement with all three typing reagents. | 1989 | 2551569 |
| a variant of herpes simplex virus type 2 strain hg52 with a 1.5 kb deletion in rl between 0 to 0.02 and 0.81 to 0.83 map units is non-neurovirulent for mice. | the virulence of a deletion variant of herpes simplex virus type 2 (hsv-2) strain hg52 has been determined by intracranial inoculation of 3-week-old balb/c mice. the variant jh2604 has a 1.5 kb deletion within each copy of the long repeat region (rl) of the genome between 0 to 0.02 and 0.81 to 0.83 map units. jh2604 is avirulent for mice compared to the parental wild-type virus, and fails to replicate in mouse brain in vivo. correction of the deletion by marker rescue resulted in the isolation o ... | 1989 | 2543755 |
| isolation of a herpes simplex virus type 2 that is retinovirulent in mice. | the virulence of a herpes simplex virus type 2 (hsv-2) isolated from the urine of a patient (sl) with acquired immunodeficiency syndrome (aids) and bilateral acute retinal necrosis (arn), was investigated in mice. the ratio of plaque forming units (pfu) in fibroblasts to the 50% lethal dose (ld50) of hsv-2(sl) in mice was 10 fold more than the pfu to ld50 ratio of a neurovirulent hsv-2, strain 186. further, hsv-2(sl) caused retinitis with and without lethal encephalitis in mice inoculated intrac ... | 1989 | 2551573 |
| inhibition of herpes simplex virus infection by tannins and related compounds. | several chemically defined plant extracts were investigated for their antiviral action on herpes simplex virus (hsv-1, hsv-2)-infected african green monkey kidney cells and human adenocarcinoma cells, using a plaque formation assay. among them, the monomeric hydrolyzable tannins, oligomeric ellagitannins and condensed tannins, having galloyl groups or hexahydroxydiphenoyl groups, had the most potent anti-hsv activity. their 50% effective doses (0.03-0.1 microgram/ml) were by two-three orders of ... | 1989 | 2552915 |
| detection of herpes simplex virus dna in a cutaneous squamous cell carcinoma by in situ hybridization. | a case of a cutaneous squamous cell carcinoma (scc) occurring at the site of a long-standing recurrent hsv infection is described. no deficit of the cell-mediated immunity was recorded. hsv2 was isolated in several viral cultures. hsv dna was visualized in the scc by in situ hybridization with biotinylated probes in paraffin-embedded tissue. the samples were negative for hpv2, 5, 16, and 18 probes. a causal relationship between hsv infection and cutaneous scc is hypothesized. | 1989 | 2552936 |
| [correlation of human papilloma virus (hpv) and herpes simplex virus-2 (hsv-2)in the viral etiology of carcinoma of uterine cervix]. | hpv dna and hsv-2 antigens were detected in a series of cervical lesions by dna hybridization and pap stain techniques in order to investigate the possible role of hpv and hsv-2 in the viral etiology of cervical carcinoma. the correlation of hpv dna sequence and hsv-2 antigens was analyzed. the results showed that the positive rates of hpv 16, 18 and 11 in cervicitis, dysplasia and cervical cancer were 13%; 0, 13%; 17%, 17%, 17%; 43%, 8%, 4%; and hsv-2 antigens were found in 41% of cervicitis, 5 ... | 1989 | 2553361 |
| proliferative t-cell response to glycoprotein b of the human herpes viruses: the influence of mhc and sequence of infection on the pattern of cross-reactivity. | oligopeptides of the highly conserved herpes virus glycoprotein b (gb) were expressed from dna fragments of the ebv gb (balf4) and hsv-2 gb open reading frames as fusion proteins with the lambda cii protein and beta-galactosidase (gz), respectively, in escherichia coli. after immunopurification using anti-gb or anti-gz affinity columns, the fusion proteins were used in vitro to stimulate human peripheral blood lymphocytes (pbl) or murine lymph node cells that have been primed with ebv, hsv-1, hs ... | 1989 | 2553584 |
| thymidine kinase deficient human cells have increased uv sensitivity in their capacity to support herpes simplex virus but normal uv sensitivity for colony formation. | a thymidine kinase deficient (tk-) and two thymidine kinase proficient (tk+) human cell lines were compared for uv sensitivity using colony-forming ability as well as their capacity to support the plaque formation of herpes simplex type 1 (hsv-1). the tk- line (143 cells) was a derivative of one of the tk+ lines (r970-5), whereas the other tk+ line (ac4 cells) was a derivative of the 143 cells obtained by transfection with purified sheared hsv-2 dna encoding the viral tk gene. 143, r970-5 and ac ... | 1989 | 2554138 |
| human topoisomerase 1 messenger rna is not destabilized by the herpes simplex virus type 2 virion-associated shut-off function. | a cdna for human topoisomerase i (topo 1) was used to identify a 4.1 kb polyadenylated topo 1 mrna in methotrexate-resistant human kb cells that are permissive for herpes simplex virus type 2 (hsv-2) infection. using these cells, no effect of the hsv-2-associated early shut-off function on levels of topo-1 mrna was observed up to 6 hours postinfection, whereas the actin mrna level was 22% cellular transcripts are susceptible. the level of several host-cell polyadenylated rnas detected as cdna cl ... | 1989 | 2554584 |
| protection and serum antibody responses in guinea-pigs and mice immunized with hsv-1 antigen preparations obtained using different detergents. | guinea-pigs immunized with a zwitterionic detergent-extracted hsv-1 antigen preparation responded with eia and nt serum antibody titres that were significantly greater than those elicited by a non-ionic detergent-extracted antigen preparation inoculated using a similar dosage schedule. following intravaginal challenge of the guinea-pigs with hsv-2 (strain sh/b), there was no statistically significant difference in the protection afforded to these animals by the two antigen preparations, although ... | 1989 | 2554607 |
| analysis of dna synthesis in herpes simplex virus infected cells by dual parameter flow cytometry. | the dna incorporation of a thymidine analogue bromodeoxyuridine (brdurd) in herpes simplex virus type 2 (hsv-2) infected cervical cancer cell lines (caski and c-33 a) was studied by dual parameter flow cytometry. hsv-2 infection resulted in an exponential increase in dna synthesis in the caski cells. in the less permissive c-33 a cells the proportion of dna-synthesizing cells cycled during hsv-2 infection. the inhibition of viral dna synthesis by phosphonoformate (pfa) was able to inhibit the vi ... | 1989 | 2554855 |
| recovery frequencies of herpes simplex virus types 1 and 2 from symptomatic and asymptomatic genital herpes cases and antiviral sensitivities of isolates. | herpes simplex viruses were isolated from 40.8 to 56.0 per cent of the patients with genital herpes. the frequency of recovery seemed to be higher in females than in males, particularly during the first episode of infection. asymptomatic shedding of the virus from female genitalias was approximately 0.7 per cent. herpes simplex virus type 2 represented 98.4 per cent of all isolates and the remaining isolates were type 1. these isolates exhibited a wide range of sensitiveness to 9-(2-hydroxyethox ... | 1989 | 2555430 |
| the herpes simplex virus type 2 (hg52) variant jh2604 has a 1488 bp deletion which eliminates neurovirulence in mice. | the herpes simplex virus type 2 (hg52) deletion variant jh2604 is avirulent (ld50 greater than 10(7) p.f.u./mouse) for mice compared to the parental wild-type virus (ld50 less than 10(2) p.f.u./mouse) and fails to replicate in vivo. jh2604 has a 1488 bp deletion within the 3 kb bamhi v fragment (0 to 0.02 and 0.81 to 0.83 map units) which removes one copy of the 17 bp direct repeat dr1 element of the 'a' sequence and terminates 522 bp upstream of the 5' end of the immediate early gene 1. in vivo ... | 1989 | 2555437 |
| evaluation of a hsv specific monoclonal antibody reagent for laboratory diagnosis of herpes simplex virus infection. | a new fitc-conjugated hsv specific monoclonal antibody reagent (syva co., palo alto, ca) was evaluated for the confirmation of hsv clinical isolates. the reagent was also compared to type-specific monoclonal antibodies for the pre-cpe detection of hsv from clinical specimens in centrifugation culture and by direct examination of specimens smears by direct immunofluorescent antibody staining (dfa). hsv was isolated from 75 of 232 specimens (32%). all 75 isolates were confirmed with both the type- ... | 1989 | 2556235 |
| herpes simplex virus stromal keratitis is not titer-dependent and does not correlate with neurovirulence. | we developed a murine model of ocular herpes simplex virus (hsv) disease which is particularly suited for testing stromal keratitis because most animals show some evidence of infection. using this model, we characterized the ocular disease patterns caused by ten recent low-passage clinical isolates of hsv-1, as well as those caused by the established laboratory strains hsv-1 kos and hsv-2 333. viral strains were evaluated for their ability to cause stromal keratitis, blepharitis, vascularization ... | 1989 | 2556353 |
| prevalence of antibodies to herpes simplex virus types 1 and 2 in pregnant women, and estimated rates of infection. | there has been a recent increase in notifications of genital herpes but it is not known whether this has been reflected in the pregnant population. we have therefore carried out a study to determine the prevalence of herpes simplex antibodies in pregnant women and to estimate the incidence of primary infection. sera were collected from 3533 women at antenatal clinics and tested for total antibodies to herpes simples virus (hsv), and if positive, for specific antibodies to hsv-2. estimates of hsv ... | 1989 | 2556492 |
| anti-herpesvirus activity of citrusinine-i, a new acridone alkaloid, and related compounds. | citrusinine-i, a new acridone alkaloid isolated from the root bark of the citrus plant (rutaceae), exhibited potent activity against herpes simplex virus (hsv) type 1 and type 2 at low concentrations relative to their cytotoxicity; 50% effective concentrations (ed50) of citrusinine-i were 0.56 micrograms/ml and 0.74 micrograms/ml against hsv-1 and hsv-2, respectively. inhibitory action was also demonstrated against cytomegalovirus (cmv) and thymidine kinase-deficient or dna polymerase mutants of ... | 1989 | 2556960 |
| active replication of human immunodeficiency virus type 1 by peripheral blood mononuclear cells following coincubation with herpes viruses. | patients with acquired immunodeficiency syndrome (aids) commonly suffer from opportunistic infections associated with members of the herpes virus family. to investigate whether certain of these other viruses might have an effect on the ability of the human immunodeficiency virus type 1 (hiv-1) to replicate, we coincubated peripheral blood mononuclear cells (pbmc) from nine hiv-1-seropositive donors with live preparations of various herpes viruses. in seven of nine cases, exposure of pbmc to prep ... | 1989 | 2557380 |
| rapid identification and typing of herpes simplex virus by a new enzyme immunoassay with peroxidase-labeled complement c1q. | a new enzyme-linked immunoassay system (tc-elisa) was developed for the rapid, specific detection and typing of herpes simplex virus (hsv) using anti-hsv immune sera and enzyme-labeled complement component c1q (p*-c1q). the method is based on viral antigen produced in hsv-infected cells being detected by simultaneous addition of immune serum and p*-c1q (elisa-cf). with this assay, it was found that hsv was detected with anti-hsv immune serum and p*-c1q and that hsv type 1 and hsv type 2 could be ... | 1989 | 2557809 |
| evidence of the non-infectivity of herpes simplex viral particles in trichomonas vaginalis. | fan et al., employing an indirect immunofluorescent antibody (ifa) technique, reported the presence of herpes simplex virus type 2 (hsv-2) in trichomonas vaginalis. however, using the same method, we found that the protozoa showed autofluorescence immediately after acetone fixation. in order to demonstrate the non-infectivity of hsv-2 in t. vaginalis, several other methods were performed in this study. trichomonads were experimentally incubated together with hsv-2 and examined after immunofluore ... | 1989 | 2558006 |
| gene ul11 of herpes simplex virus type 1 encodes a virion protein which is myristylated. | we have investigated whether herpes simplex virus (hsv) contains structural polypeptides which are modified by myristic acid. we demonstrate that herpes simplex virions contain a family of myristylated proteins, mr approximately 13,000 to 16,000. these were mapped, using hsv-1/hsv-2 intertypic recombinants, to 0.130 to 0.204 map units on the virus genome. using anti-peptide sera, raised against the carboxyterminus of the predicted ul11 gene product, we have established that the myristylated viri ... | 1989 | 2558153 |
| herpes simplex virus in oral mucosal ulcers in patients with hematological malignancy. | developments of oral mucosal ulcers induced by herpes simplex virus (hsv) were studied in patients with hematologic malignancy. herpes simplex virus type-1 (hsv-1) was identified by immunological staining using virus-specific monoclonal antibodies in the epithelial cells of such ulcers from two patients with malignant lymphoma (ml), three with acute myeloblastic leukemia (aml), one with refractomy anemia with excess blasts, two with chronic myelocytic leukemia (cml), one with acute lymphoblastic ... | 1989 | 2558241 |
| simian alphaherpesviruses and their relation to the human herpes simplex viruses. | biochemical and immunological properties of structural and non-structural polypeptides of the human simplex viruses (hsv1 and hsv2) and four related herpesviruses of non-human primates [herpesvirus simiae (b virus), h. cercopithicus (sa8), h. saimiri 1 (hvs 1), and h. ateles 1 (hva 1)] were compared. using a radioimmunoassay (ria), the presence of antigenic determinants shared among all six viruses was demonstrated. the relative degree of antigenic cross-reactivity among these viruses was furthe ... | 1989 | 2558632 |
| histopathologic study of herpes virus-induced retinitis in athymic balb/c mice: evidence for an immunopathogenic process. | in order to determine whether antiviral immunity is pathogenic in mouse eyes, hsv-1 was injected into the anterior chamber of one eye of adult athymic balb/c mice. the eyes of these t cell deficient mice were examined clinically and histopathologically for ocular disease. the anterior segment of injected eyes developed progressive inflammatory reactions that eventually destroyed the ciliary body and then progressed to the posterior compartment where partial necrosis occurred, but only in the inn ... | 1989 | 2558848 |
| increased efficacy of ganciclovir in combination with foscarnet against cytomegalovirus and herpes simplex virus type 2 in vitro and in vivo. | in tissue culture, efficacy against either murine cmv or hsv-2 was increased 27-fold for the acyclic nucleoside ganciclovir and 3-fold for foscarnet (trisodium phosphonoformate) when the 2 drugs were combined; whereas against human cmv, efficacy was increased 3-fold for both drugs. in mice, efficacy was increased 2-fold for ganciclovir and 4- to 5-fold for foscarnet when used in combination against either murine cmv or hsv-2. these results suggest an additive interaction between the two drugs in ... | 1989 | 2559657 |
| synthesis and biological activity of dansyl thymidines. | the synthesis of thymidines selectively dansylated in the 3'- and 5'-positions is reported. the biological investigation showed that these fluorescent nucleosides behave as competitive inhibitors of thymidine kinase (tk) from herpes simplex virus (hsv) and are endowed with a certain degree of antiviral activity against hsv-2 and hsv-1. | 1989 | 2559740 |
| anti-herpesvirus activity of carbocyclic oxetanocin g in vitro. | a series of new compounds, carbocyclic oxetanocins, have been synthesized and their anti-herpesvirus activity determined. carbocyclic oxetanocin g (oxt-g) was most active against herpes simplex virus (hsv) and human cytomegalovirus (hcmv) among carbocyclic oxetanocins tested; the median effective concentrations (ec50) for hsv-1, -2, and hcmv were 0.23, 0.04 and 0.40 micrograms/ml, respectively. the ec50 value of carbocyclic oxt-g against hsv-2 was significantly lower than those of acyclovir, gan ... | 1989 | 2559911 |
| the effect of cimetidine on survival of mice infected with herpes simplex virus type 2, murine encephalomyelitis virus and vesicular stomatitis virus infections. | the effect of cimetidine on survival was investigated in mice infected with herpes simplex virus type 2 (hsv-2), murine encephalomyelitis virus (gd-vii), and vesicular stomatitis virus (vsv). balb/c mice, 5 weeks of age, were injected intraperitoneally (i.p.) with 5.5 x 10(5) plaque-forming units (pfu) of virus/0.5 ml, and cimetidine (1 mg/0.5 ml) was administered simultaneously. the survival rates of 80% and 85% in the cimetidine groups were significantly greater than the 10% and 23% for the co ... | 1989 | 2561403 |
| [epidemiological evaluations of human immunodeficiency virus, herpes simplex virus type 1 and 2 and cytomegalovirus infections in drug addicts]. | eighty-eight drug addicts from the "ban center" in torre annunziata (naples) and 88 normal subjects pair-matched for age and sex were tested for igg to human immunodeficiency virus (hiv), herpes simplex virus (hsv) type 1 and 2 and cytomegalovirus (cmv). a high prevalence of subjects with antibodies to hsv-1 and cmv (80.7% and 65.9%) were recorded in the control group testifying to the high level of these infections in campania. prevalences were higher in drug addicts, and drug abuse was identif ... | 1989 | 2562004 |
| [characteristics and role of the gluteal herpes in a female population]. | based on the fact that the gluteal herpes may constitute the clinical expression of the reactivation of a herpes simplex virus latent at the sacral lymph node, we investigated a group of women who were carriers of gluteal herpetic infection, the characteristics of the infection, the virus type principally associated to it, and its possible relation with the genital herpes. forty one women with gluteal herpes verified by virologic laboratory were studied. 75.7% of these women had had in addition ... | 1989 | 2562294 |
| [evaluation of 2 commercial monoclonal antibody kits for the identification of herpes simplex virus]. | two commercial monoclonal antibody kits (virgo and mikrotrak for the identification of herpes simplex virus (hsv) have been assessed. in 65 positive hsv cultures from clinical samples, 25 strains (38%) were identified as hsv1 with both kits. the virgo kit identified 37 strains (57%) as hsv2, and did not identify the remaining 3 (5%). with the microtrak kit, 34 strains (52%) corresponded to type 2, 2 (3%) strains remained nonidentified, and in the remaining 4 isolates (6%) both viral types were i ... | 1989 | 2562319 |
| [maternal genital herpes: virus isolation during the third trimester of pregnancy and at delivery]. | we followed 42 pregnant women at high risk for genital herpes infection. viral isolation was attempted weekly from the 34th week of pregnancy. one or more episodes of herpes infection developed in 74% of women. the virus was isolated in 28% after 34 weeks and in 22% after 37 weeks. a prophylactic cesarean section was performed in the latter group; only one of these women had a positive viral isolation at delivery. the hsv-2 of virus was found in 83% of cases and hsv-1 in 17%. | 1989 | 2562415 |
| hsv hepatitis in the mouse: a light and electron microscopic study with immunohistology and in situ hybridization. | in order to characterize better the morphology and immune response in acute necrotizing hsv infection, murine hsv hepatitis was examined. balb/c mice were inoculated intraperitoneally with 10(6) plaque-forming units (pfu) of hsv-1 (lenette) and hsv-2 (d316). in both groups half the animals were pretreated with silica particles to block macrophage function. up to 6 days after infection four mice from each group were sacrificed at daily intervals and the livers were examined by light and electron ... | 1989 | 2567083 |
| some immunological characteristics of subjects suffering from frequent herpes simplex virus recrudescences. | some parameters of specific and non-specific immunity were tested in a group of 44 subjects suffering from frequent herpes simplex type 1 (hsv-1) or herpes simplex type 2 (hsv-2) recrudescences. the tests performed included determinations of (i) hsv complement-independent and complement-dependent neutralizing antibodies, (ii) antibodies to glycoprotein c of hsv-1 and glycoprotein g of hsv-2, (iii) antibodies to viral capsid and early antigens of epstein-barr virus, (iv) antibodies to tetanus tox ... | 1989 | 2576584 |
| lack of antiviral activity of ketoconazole alone or in combination with the acyclic nucleoside ganciclovir against a herpes virus type 2 infection in mice. | ketoconazole and ganciclovir were tested for antiviral activity, each alone and in combination, against a herpes simplex virus type 2 (hsv-2) systemic infection in swiss-webster mice. when given once daily for 5 days starting 24 hr after infection, the ed50 for ketoconazole either alone or in combination was greater than 60 mg/kg; for ganciclovir, the ed50 was 7.1 mg/kg alone and 10.8 mg/kg in combination. thus, ketoconazole did not potentiate or antagonize the antiviral activity of an acyclic n ... | 1989 | 2576599 |
| gynaecological infections and strategies for treatment. | frequently, encountered gynaecologic infections and pathogens involved are presented. a review is given of bartholinitis, vulvovaginitis-bacterial vaginosis, candidiasis, trichomoniasis cervicitis and pelvic inflammatory disease: neisseria gonorrhoeae, chlamydia trachomatis and herpes simplex virus 2. of each infection a thorough description of clinical symptoms and diagnostic criteria is followed by up-to-date treatment advices. for vulvovaginitis, cervicitis and pelvic inflammatory disease ant ... | 1990 | 2075077 |
| [lesions of the spinal cord and spinal roots in human immunodeficiency virus infection]. | spinal cord lesions, although far less frequent than brain lesions, are not uncommon in aids. almost all diffuse or multifocal pathological processes involving the central nervous system and/or leptomeninges may also affect the spinal cord. however, some of them involve it predominantly causing specific, clinically overt, myelopathic diseases. the most characteristic of these is vacuolar myelopathy which usually manifests as progressive spastic ataxia. its incidence varies in different pathologi ... | 1990 | 2077614 |
| effect of the estrous cycle on susceptibility of female mice to intravaginal inoculation of herpes simplex virus type 2 (hsv-2). | in order to explore the effect of sexual maturity on the susceptibility of mice to genital herpesvirus infections, mice were separated into the four stages of the estrous cycle and inoculated intravaginally with varying doses of hsv-2, strain 186. deaths were observed as indicators of susceptibility and were recorded as follows: proestrous, 33%; estrous, 16%; metestrous, 9% and diestrous, 75%. to determine the course of infection in animals inoculated at different stages of estrous, cotton swabs ... | 1990 | 2088207 |
| protection from herpes simplex virus type 1 lethal and latent infections by secreted recombinant glycoprotein b constitutively expressed in human cells with a bk virus episomal vector. | the herpes simplex virus type 1 (hsv-1) glycoprotein b (gb-1) gene, deleted of 639 nucleotides that encode the transmembrane anchor sequence and reconstructed with the extramembrane and intracytoplasmic domains, was cloned under control of the rous sarcoma virus long terminal repeat in the episomal replicating vector prp-rsv, which contains the origin of replication and early region of the human papovavirus bk as well as a cdna for a mutant mouse dihydrofolate reductase that is resistant to meth ... | 1990 | 2152829 |
| fluoro carbocyclic nucleosides: synthesis and antiviral activity of 2'- and 6'-fluoro carbocyclic pyrimidine nucleosides including carbocyclic 1-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)-5-methyluracil and carbocyclic 1-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)-5-iodouracil. | the racemic carbocyclic 2'-fluoroarabinosyl pyrimidine nucleosides 8, 9 (c-fiau), 12, and 13 (c-fmau) and the 2'-fluororibosyl pyrimidine nucleosides 17, 20, and 21 were prepared from their respective protected 2'-fluoro amino diols 5 and 14. the carbocyclic 2'-2'-difluorothymidine analogue 27 was obtained from the protected difluoro amino diol 24 which was prepared from the ketone 23 and (diethylamino)sulfur trifluoride (dast). the chiral carbocyclic 2'-deoxy-6'-fluorouridines 33, 34, 38, and 3 ... | 1990 | 2153201 |
| identification and characterization of the herpes simplex virus type 2 gene encoding the essential capsid protein icp32/vp19c. | we describe the characterization of the herpes simplex virus type 2 (hsv-2) gene encoding infected cell protein 32 (icp32) and virion protein 19c (vp19c). we also demonstrate that the hsv-1 ul38/orf.553 open reading frame (orf), which has been shown to specify a viral protein essential for capsid formation (b. pertuiset, m. boccara, j. cebrian, n. berthelot, s. chousterman, f. puvian-dutilleul, j. sisman, and p. sheldrick, j. virol. 63: 2169-2179, 1989), must encode the cognate hsv type 1 (hsv-1 ... | 1990 | 2154597 |
| localization on the herpes simplex virus type 1 genome of a region encoding proteins involved in adsorption to the cellular receptor. | we have previously shown that aminoglycosides such as neomycin and the polyamino acids polylysine and polyarginine selectively inhibit the binding of herpes simplex virus type 1 (hsv-1) to the cellular receptor, whereas hsv-2 infection is unaffected. in the present study we took advantage of this difference between hsv-1 and hsv-2 by using hsv(-1)-hsv(-2) intertypic recombinants to locate a region on the hsv-1 genome encoding proteins affecting the binding of the virion to the cellular receptor. ... | 1990 | 2154609 |
| in vitro synthesis and processing of herpes simplex virus type 2 gg-2, using cell-free transcription and translation systems. | translation of in vitro-synthesized herpes simplex virus type 2 (hsv-2) gg-2 mrna in a reticulocyte lysate system was used to study the processing of hsv-2 gg-2. in the presence of canine pancreatic microsomal membranes, a single species that is protected from trypsin digestion was detected. this product comigrates with the 104,000-mr (104k) high mannose intermediate seen in hsv-2-infected-cell lysates. endo-beta-n-acetylglucosaminidase h treatment of the in vitro-synthesized 104k protein yielde ... | 1990 | 2154614 |
| restriction endonuclease analysis of deoxyribonucleic acid (dna) from genital herpes simplex virus type 2 isolates in thailand. | local herpes simplex virus (hsv) isolates were characterized by the use of bamhi, kpni, and ecori restriction endonucleases. analysis of 120 clinical isolates by observation of presence or absence of cleavage sites and of mobility variation in deoxyribonucleic acid (dna) fragments from the terminal and joint regions of the hsv genome revealed that 112 (93.3%) were type-2 (hsv-2) and the others were type-1 (hsv-1), mixed hsv-2 and hsv-1, and mixed hsv-2 strains. the genomes of hsv-2 isolates were ... | 1990 | 2154866 |
| structural organization of the conserved gene block of herpesvirus saimiri coding for dna polymerase, glycoprotein b, and major dna binding protein. | lymphotropic herpesviruses such as epstein-barr virus and herpesvirus saimiri are commonly grouped as gamma-herpesviruses, although overall genome organization and numerous biological properties are quite different in the viruses. to define the relationship more precisely, we sequenced the kpnl fragments f (6.5 kb) and c (9.8 kb) of the h.saimiri strain no. 11 genome; these dna fragments were found to contain the genes coding for equivalents of the major dna binding protein, a putative glycoprot ... | 1990 | 2154888 |
| influence of vehicle on topical efficacy of 2-acetylpyridine thiosemicarbazone and related derivatives on in vivo type 2 herpes simplex virus infections. | 2-acetylpyridine semicarbazone (apsc), 2-acetylpyridine thiosemicarbazone (aptsc) and 2-acetylpyridine-4-methyl-3-thiosemicarbazonoe (apmtsc) were evaluated against type-2 herpes simplex virus (hsv-2)-induced genitalis and encephalitis in mice and guinea pigs. the antiviral activity of these compounds was compared with that of acyclovir. with 1,3-butanediol as a topical treatment vehicle, 1% apsc and aptsc showed significant activity against the genital infection in mice, as evidenced by increas ... | 1990 | 2155092 |
| transfer of ul41, the gene controlling virion-associated host cell shutoff, between different strains of herpes simplex virus. | studies with mutant viruses have suggested that the product of gene ul41 of herpes simplex virus type 1 (hsv-1) controls the virion-mediated inhibition of cellular protein synthesis as well as the rate of degradation of viral mrnas. hsv-1 strain 17+ has a weak host shutoff function, whereas hsv-2 strain g shuts off strongly. a gene of hsv-2(g), judged from its position in the genome to be the probable analogue of gene ul41 of hsv-1, was inserted into the nonessential thymidine kinase gene of hsv ... | 1990 | 2155295 |
| nmr investigation of cell cultures: early detection of infection by herpes simplex virus type 2 and transformation. | the water proton nuclear magnetic resonance (nmr) spin-lattice relaxation rates were measured in suspension of uninfected and herpes simplex virus type 2-infected wi-38 diploid cells and shown to allow reliable detection of (i) the early signs of productive infection, and (ii) the tendency to undergo either spontaneous or virus-induced transformation. in addition, nmr investigation of several transformed cell lines currently and previously established from wi-38 cells clearly indicated that the ... | 1990 | 2155373 |
| sexually transmitted diseases in victims of rape. | the risk of acquiring a sexually transmitted disease as a result of rape is not known, in part because it is difficult to ascertain whether infections were present before the assault or acquired during it. to investigate this question, we examined female victims of rape within 72 hours of the assault and again at least one week after the assault. of the 204 girls and women initially examined within 72 hours of the rape, 88 (43 percent) were found to have at least one sexually transmitted disease ... | 1990 | 2155389 |
| role of dna topoisomerase i in the replication of herpes simplex virus type 2. | camptothecin (cpt), a specific inhibitor of dna topoisomerase i, effectively inhibited the replication of herpes simplex virus type 2 (hsv-2). cpt was found to reduce the level of immediate early viral mrna in a dose-response manner. on the other hand, we could not find any evidence that a novel dna topoisomerase i was induced in hsv-2-infected cells. our observations suggest that host cell dna topoisomerase i is involved in the replication of hsv-2. | 1990 | 2155595 |
| neutralizing antibody to herpes simplex in pregnant women and their neonates. | the neutralizing antibody (nab) titer against herpes simplex virus (hsv) was determined in blood obtained at term delivery in 76 women with documented genital hsv infection. maternal and cord blood nab titers against hsv-1 and hsv-2 displayed significant correlation (r = .88 and r = 0.89, respectively). in 33% of the paired samples, the neonatal nab titer against hsv-1 exceeded the concomitant maternal titer, and in 59% the maternal and neonatal nab titers against hsv-1 were equal. in 28% of the ... | 1990 | 2156030 |
| carbocyclic 5-iodo-2'-deoxyuridine (c-idu) and carbocyclic (e)-5-(2-bromovinyl)-2'-deoxyuridine (c-bvdu) as unique examples of chiral molecules where the two enantiomeric forms are biologically active: interaction of the (+)- and (-)-enantiomers of c-idu and c-bvdu with the thymidine kinase of herpes simplex virus type 1. | the (+)- and (-)-enantiomers of the carbocyclic analogues of (e)-5-(2-bromovinyl)-2'-deoxyuridine (c-bvdu) and 5-iodo-2'-deoxyuridine (c-idu) were synthesized by separate routes. both the (+)- and (-)-enantiomers of c-bvdu and c-idu were markedly inhibitory to herpes simplex virus type 1 (hsv-1) replication. (+)-c-bvdu and (+)-c-idu were as inhibitory to hsv-1 as the racemic (+/-)-c-bvdu and (+/-)-c-idu, respectively, whereas the (-)-enantiomers were only 10-fold less active. also, the (+)- and ... | 1990 | 2156153 |
| phenotypic properties of herpesvirus-transformed cells with high tumorigenic and metastatic ability. | herpes simplex virus type 2-transformed hamster embryo fibroblasts (333-8-9 cells) produce increased plasminogen activator (pa) compared with normal hamster cells. these cells produce undifferentiated fibrosarcomas at the inoculation site in newborn hamsters, and metastasize to the lungs. using a direct pa assay, in which 125i-labeled plasminogen is cleaved, the optimum ph and osmolarity for detection of the 333-8-9 extracellular pa were ph 8.9 and approximately 150 mosmol. secretion of enzyme d ... | 1990 | 2156388 |
| risk factors for cervical human papillomavirus and herpes simplex virus infections in greenland and denmark: a population-based study. | risk factors for genital human papillomavirus (hpv) types 6/11 and 16/18 and any hpv type as well as for herpes simplex virus type 2 (hsv-2) infections were investigated in a population-based study of 1,600 randomly selected women (20-39 years) from godthåb (native, nuuk), greenland, and nykøbing falster, denmark. a total of 586 greenlandic women and 661 danish women were included, respectively. they all had a personal interview and a gynecologic examination with cervical smear and swab for hpv ... | 1990 | 2156420 |
| herpes simplex virus type 2 meningoencephalitis resistant to acyclovir in a patient with aids. | a case is reported of relapsing fatal meningoencephalitis caused by a neurovirulent thymidine kinase-positive (tk+) type 2 herpes simplex virus (hsv) that developed thymidine kinase deficiency (tk-) during intravenous acyclovir therapy. a patient with aids was admitted for acyclovir treatment of a persistent perirectal herpetic ulcer. he subsequently developed meningoencephalitis. a tk+ type 2 hsv was isolated from a brain biopsy specimen. a progressive and fatal relapse occurred, and a tk- type ... | 1990 | 2156946 |
| acyclic purine phosphonate analogues as antiviral agents. synthesis and structure-activity relationships. | a series of 9-(phosphonoalkyl)purines, which are analogues of 9-[2-(phosphonomethoxy)ethyl]purines (guanine, pmeg, 1; adenine, pmea, 2), were synthesized. the analogues were tested for activity against herpes simplex virus types 1 and 2 (hsv-1 and hsv-2), human cytomegalovirus (hcmv), rauscher murine leukemia virus (r-mulv), and human immunodeficiency virus type 1 (hiv-1). with variations in the length of the alkyl chain, the optimal activity was achieved with two carbons between the purine base ... | 1990 | 2157012 |
| identification of c3b-binding regions on herpes simplex virus type 2 glycoprotein c. | glycoprotein c from herpes simplex viruses types 1 and 2 (gc-1 and gc-2) acts as a receptor for the c3b fragment of the third component of complement. our goal is to identify domains on gc involved in c3b receptor activity. here, we used in-frame linker-insertion mutagenesis of the cloned gene for gc-2 to identify regions of the protein involved in c3b binding. we constructed 41 mutants of gc-2, each having a single, double, or triple insertion of four amino acids at sites spread across the prot ... | 1990 | 2157859 |
| stereospecific synthesis and antiviral properties of different enantiomerically pure carbocyclic 2'-deoxyribonucleoside analogues derived from common chiral pools: (+)-(1r,5s)- and (-)-(1s,5r)-2-oxabicyclo[3.3.0]oct-6-en-3-one. | enantiomerically pure (+)- and (-)-carbocyclic thymidine, (-)-carbocyclic 3'-epi-thymidine, (+)-carbocyclic 3'-deoxy-3'-azidothymidine, (+)-carbocyclic 2,3'-o-anhydrothymidine, (+)-carbocyclic 3'-o,6'-methylenethymidine, and (+)-(6's)-carbocyclic 6'-methylthymidine were synthesized in a stereospecific manner from common chiral pools of (+)-(1r,5s)- and (-)-(1s,5r)-2-oxabicyclo[3.3.0]oct-6-en-3-one and evaluated for antiviral activity. (+)-carbathymidine and, to a lesser extent, (+)-carbocyclic 2 ... | 1990 | 2158560 |
| (+-)-(1 alpha,2 beta,3 alpha)-9-[2,3-bis(hydroxymethyl)-cyclobutyl] guanine [(+-)-bhcg or sq 33,054]: a potent and selective inhibitor of herpesviruses. | (+-)-(1 alpha,2 beta,3 alpha)-9-[2,3-bis(hydroxymethyl)cyclobutyl] guanine [(+-)-bhcg or sq 33,054] is a newly synthesized nucleoside analog with potent and selective antiviral activity against members of the herpesvirus group, including human cytomegalovirus. the activity against a thymidine kinase deficient hsv-2 mutant was 25-fold poorer than against the parent virus, suggesting that phosphorylation is an important prerequisite for antiviral activity against hsv-2. (+-)-bhcg is readily phosph ... | 1990 | 2159261 |
| soluble forms of herpes simplex virus glycoprotein d bind to a limited number of cell surface receptors and inhibit virus entry into cells. | herpes simplex virus type 1 (hsv-1) and hsv-2 plaque production was inhibited by treating cells with soluble forms of hsv-1 glycoprotein d (gd-1t) and hsv-2 glycoprotein d (gd-2t). both glycoproteins inhibited entry of hsv-1 and hsv-2 without affecting virus adsorption. in contrast, a soluble form of hsv-2 glycoprotein b had no effect on virus entry into cells. specific binding of gd-1t and gd-2t to cells was saturable, and approximately 4 x 10(5) to 5 x 10(5) molecules bound per cell. binding o ... | 1990 | 2159532 |
| [diagnosis of varicella-zoster virus (vzv) infection by using fitc-labeled monoclonal antibodies]. | anti-varicella zoster virus (vzv) mouse monoclonal antibodies conjugated with fluorescein isothiocyanate were evaluated for their usefulness as a practical diagnostic tool in the clinical field by examining cells infected with isolated herpes viruses and 431 clinical samples. the kit stained clearly the cells infected with 14 isolated vzv strains without cross reaction to 15 isolated herpes simplex virus type-1 strains (hsv-1) and 14 type-2 (hsv-2) strains. in clinical specimens, viral antigens ... | 1990 | 2159967 |
| synthesis and antiviral activity of (s)-9-[4-hydroxy-3-(phosphonomethoxy)butyl]guanine. | the synthesis of (s)-9-[4-hydroxy-3-(phosphonomethoxy)butyl]guanine (3), starting from (s)-4-(2-hydroxyethyl)-2,2-dimethyl-1,3-dioxolane (4), is described. alkylation of trityl derivative 7 with (diethylphosphono)methyl triflate provided phosphonate 8, which was readily converted to mesylate 12 in three steps. nucleophilic substitution of the mesylate group of 12 by 2-amino-6-chloropurine sodium salt led to (s)-2-amino-6-chloro-9-[3-[(diethyl-phosphono)methoxy]-4-(tetrahydro- 2h-pyran-2-yloxy)bu ... | 1990 | 2160539 |
| viral activation of the coagulation cascade: molecular interactions at the surface of infected endothelial cells. | herpesviral infection of endothelial cells (ecs) induces arterial injury. we now demonstrate that such infection promoted enhanced monocyte-endothelial adhesion. enhanced adhesion was blocked by monoclonal antibodies to the viral-encoded cell surface glycoprotein gc but not by antibodies to gd or ge. adhesion was also blocked by treating ecs with specific thrombin inhibitors or by growing cells in prothrombin-depleted serum. we found that gc bound and promoted activation of factor x on infected ... | 1990 | 2160855 |
| nucleotide sequences responsible for the inability of a herpes simplex virus type 2 strain to grow in human lymphocytes are identical to those responsible for its inability to grow in mouse tissues following ocular infection. | a study was undertaken to determine whether genes associated with herpes simplex virus (hsv) neuroinvasiveness in mice influence the growth of hsv in man, the virus's natural host. hsv-2(186), a nonneuroinvasive hsv strain, was found to replicate poorly (less than 3-fold) in cultures of phytohemagglutinin (pha) stimulated human peripheral blood mononuclear cells (pbmc). in contrast, seven other hsv strains all multiplied 40- to 100-fold. the paucity of hsv-2(186) growth in pbmc was not due to a ... | 1990 | 2161143 |
| nucleotide sequence of the herpes simplex virus type-2 syn gene that causes cell fusion. | the nucleotide (nt) sequence of the entire coding region of the syn gene of herpes simplex virus type 2 (hsv-2) is reported. the amino acid (aa) sequence deduced from the nt sequence has been compared to analogous herpes simplex virus type 1 (hsv-1) syn gene that causes cell fusion. the aa sequences are 82.8% homologous and contain a highly conserved region of 90 aa. these results identify some of the conserved regions in the coding sequences between the two types. | 1990 | 2161382 |
| herpes simplex virus-encoded ribonucleotide reductase: evidence for the dissociation/reassociation of the holoenzyme. | 35s-labeled cells infected with herpes simplex virus type 1 (hsv-1), temperature-sensitive (ts) mutant ts 1222 were used as a source of the large subunit of the viral ribonucleotide reductase (rr) to investigate the binding of the large (rr1) and small (rr2) subunits in the active enzyme. mixing 35s-labeled rr1 from ts 1222 with unlabeled rr1/rr2 complex from wild type (wt) infected cells resulted in the formation of a complex between 35s-labeled rr1 and unlabeled rr2, indicating that the comple ... | 1990 | 2161585 |
| comparative dna sequence analysis of the host shutoff genes of different strains of herpes simplex virus: type 2 strain hg52 encodes a truncated ul41 product. | herpes simplex virus (hsv) particles contain a factor which can shut off host protein synthesis during the earliest stages of infection. the efficiency of shutoff varies between different strains of virus, type 2 strains being generally more active than type 1 strains. however, hsv-2 strain hg52 is deficient in host cell shutoff. we have investigated the basis of the different shutoff phenotypes of a strong shutoff strain (hsv-2 strain g), a weak shutoff virus (hsv-1 strain 17 syn+) and hg52 by ... | 1990 | 2161906 |
| the herpes simplex virus us11 open reading frame encodes a sequence-specific rna-binding protein. | herpes simplex virus 1- and 2 (hsv-1 and hsv-2)-infected cell extracts but not uninfected cell extracts contain an rna-binding activity for an in vitro-transcribed sequence from the domains of the hsv-1 us11 and alpha 47 genes. the transcript of this sequence has not been detected in infected cells. the binding is sequence and secondary structure specific and protects approximately 95 nucleotides from rnase digestion. analyses of hsv-1 x hsv-2 recombinants and hsv-1 deletion mutants mapped the f ... | 1990 | 2161949 |
| detection of varicella-zoster virus dna by field-inversion gel electrophoresis. | a new method for detection of varicella-zoster virus (vzv) dna using field-inversion gel electrophoresis (fige) was devised. vzv-genomic dna could be differentiated from the host cell dna of human embryonic lung (hel) fibroblasts infected with vzv under electrophoretic conditions allowing resolution of linear and double-stranded dnas in the 49-230 kilobase pairs (kb) range. the detection of vzv-genomic dna from infected hel cells was successful regardless of whether the vzv was a laboratory stra ... | 1990 | 2161996 |
| treatment of primary and recurrent herpes simplex virus type 2 induced meningitis with acyclovir. | we describe therapy with acyclovir in 1 patient with acute meningitis induced by herpes simplex virus type 2 (hsv-2) and in 1 patient with ascending myelitis in connection with meningitis after a primary genital hsv-2 infection. in addition, intermittent or continuous acyclovir prophylaxis against meningitis was employed in 3 patients with recurrent meningitis of probable hsv-2 etiology. possible beneficial effects of the treatment were seen. | 1990 | 2162558 |
| the frequency of unrecognized type 2 herpes simplex virus infection among women. implications for the control of genital herpes. | to evaluate the prevalence of symptomatic versus asymptomatic or unrecognized type 2 herpes simplex virus (hsv-2) infection, the authors performed physical examination, viral cultures, and type-specific serologic assays in 776 randomly selected women attending an std clinic and 636 female university students. forty-six percent of women attending the std clinic compared with 8.8% of the university students had serologic evidence of hsv-2 infection. clinical or historical evidence of genital herpe ... | 1990 | 2163115 |
| inhibition of herpes simplex virus type 2 growth by phosphorothioate oligodeoxynucleotides. | phosphorothioate homo-oligodeoxynucleotides were found to be potent inhibitors of herpes simplex virus type 2 (hsv-2) but less potent for hsv-1 in cell culture studies. oligomers with longer chain lengths were more active against hsv-2 than those with shorter ones. of all the compounds examined, the 28-mer phosphorothioate homo-oligodeoxynucleotides were the strongest inhibitors of hsv-2. the degree of inhibition was related to the base moiety on the order of deoxycytidine = thymidine greater th ... | 1990 | 2163243 |
| analysis of the role of antibody-dependent cellular cytotoxic antibody activity in murine neonatal herpes simplex virus infection with antibodies to synthetic peptides of glycoprotein d and monoclonal antibodies to glycoprotein b. | the role of antibody in neonatal herpes simplex virus (hsv) infection remains controversial. a battery of well-characterized monoclonal antibodies to hsv glycoprotein b (gb), and polyclonal antibodies against synthetic peptides of predicted epitopes of hsv glycoprotein d (gd) were used to determine in vitro functional activity and association with protection against lethal infection in a murine model of neonatal hsv disease. antiviral neutralization activity of hsv was not associated with antibo ... | 1990 | 2164044 |
| recurrent male herpes genitalis with two different strains of herpes simplex virus type 2. | a case of a 50-year-old man with recurrent herpes genitalis with two different isolated strains of herpes simplex virus (hsv) type 2 is reported. morphologically, two types of cytopathic effects (cpe) induced by viruses were observed in the vero cell cultures, one being a syncytial giant cell formation and the other a rounded cell formation without cell adhesion resembling the cpe induced by ordinary hsv type 2. in this case, it is clinically interesting that the patient complained of persistent ... | 1990 | 2164052 |
| no association between herpes simplex virus type-2 seropositivity or anogenital lesions and hiv seroconversion among homosexual men. | recent reports have suggested that hsv-2 infection and associated anogenital ulcerations represent an important risk factor for acquisition of hiv infection. although this is an appealing biological hypothesis, inferences drawn for homosexual men, as well as other at-risk populations, must be made after careful consideration of methods to control for potential confounding data. this report utilized a nested case-control study in which 49 homosexual hiv seroconverters were compared to 49 homosexu ... | 1990 | 2164082 |
| genomic variation of herpes simplex virus type 2 isolates analysed by hybridization after electroblotting from polyacrylamide gels. | genomic variation of herpes simplex virus type 2 (hsv-2) strains was analysed by polyacrylamide gradient gel electrophoresis and subsequent hybridization to cloned hsv-2 sequences. two probes were used, one from the l-segment and one from the s-segment of the hsv-2 genome. the probes did not contain a-repeat sequences. hybridization to the specific sequences in individual dna fragments obtained by use of the frequently cleaving restriction endonuclease alu i revealed variations in the genome not ... | 1990 | 2164145 |
| identification of the cross-reactive antigens between marek's disease virus and pseudorabies virus by monoclonal antibodies. | among the 33 monoclonal antibodies (mabs) against pseudorabies virus (prv) examined, three mabs (24-17, 74-26, and 8) were found to react with cells infected with marek's disease virus (mdv)-related viruses by immunofluorescence test. two of the mabs (24-17 and 74-26) reacted with the nuclei of cells infected with mdv serotype 1 (mdv1), mdv serotype 2 (mdv2), and herpesvirus of turkeys (hvt), whereas mab 8 reacted with the cytoplasm of mdv2- and hvt-infected cells. however, none of the mabs agai ... | 1990 | 2164393 |
| serologic type conversion of a herpes simplex virus type 1 (hsv-1) to an hsv-2 epitope caused by a single amino acid substitution in glycoprotein c. | a monoclonal antibody to herpes simplex virus type 2 glycoprotein c (gc-2) did not recognize wild-type herpes simplex virus type 1 gc (gc-1) but did recognize a mutant gc-1 molecule. this conversion from a type 1 to a type 2 epitope was shown to be due to a single amino acid substitution in gc-1. | 1990 | 2164613 |
| serum amyloid a protein-related mrna expression in herpes simplex virus type 2-transformed hamster cells. | a nearly full-length cdna very similar to murine serum amyloid a protein was cloned from herpes simplex virus type 2-transformed hamster cells. the expression of mrna was constitutive in these cells and could be superinduced by conditioned medium. higher rates of nuclear runoff transcription in transformed and induced cells indicated some regulation at the transcriptional level. | 1990 | 2164641 |
| a prospective evaluation of primary genital herpes simplex virus type 2 infections acquired during pregnancy. | in order to study the epidemiology of herpes simplex type 2 (hsv-2) infections during pregnancy, we used an enzyme immunoassay to detect type-specific antibodies to hsv-2 glycoprotein g in serial blood samples obtained from a cohort of 1891 pregnant women. blood samples obtained at about 17 and 32 weeks of gestation and at the time of delivery were assessed for antibody to hsv-2 glycoprotein g in order to evaluate the prevalence of past infections with hsv-2 and the rate of acquisition of hsv-2 ... | 1990 | 2164656 |
| immortalization of rabbit vascular smooth muscle cells after transfection with a fragment of the bglii n region of herpes simplex virus type 2 dna. | rabbit arterial smooth muscle cells were transfected with the pbc24neo plasmid dna which consists of a sequence of 790 base pairs from the bglii n fragment of herpes simplex virus type 2 dna linked to the neo resistance gene. selection in g418-containing medium resulted in eleven immortalized clones which showed increased growth rate and saturation densities. one of the eleven clones maintained the transforming dna sequence integrated in the cellular genome and showed continuous resistance to g4 ... | 1990 | 2165042 |
| nucleocapsid, nuclear association, and genome location of the herpes simplex virus type 2 38-kd dna-binding protein. | a previously-described herpes simplex virus type 2 dna-binding protein with a molecular size of 38 kd has been further characterized. using purified nucleocapsids, a dnase release assay, and intertypic recombinants, this protein was found to be a component of the nucleocapsid, intimately associated with the nuclear matrix, and encoded between 0.605 and 0.720 on the herpes simplex virus type 2 genome. | 1990 | 2165049 |
| epidemiology of genital herpes in pittsburgh: serologic, sexual, and racial correlates of apparent and inapparent herpes simplex infections. | women attending family planning clinics in western pennsylvania were enrolled into a prospective epidemiologic study of herpes simplex virus (hsv) infection. detection of antibodies to hsv-1 and hsv-2 was based on an immunodot assay using type-specific glycoproteins gg-1 and gg-2. serologic and historical data at enrollment were analyzed for 4527 subjects; the seroprevalence of hsv-2 was 21.6%. by multivariate analysis, hsv-2 infection as determined by seroprevalence was significantly and indepe ... | 1990 | 2165102 |
| a cross-sectional study of herpes simplex virus types 1 and 2 in college students: occurrence and determinants of infection. | occurrence rates and determinants of infection with herpes simplex virus types 1 and 2 (hsv-1 and hsv-2) were measured in first- and fourth-year undergraduate students at a state university. this cross-sectional multistage probability sample survey included sociodemographic characteristics, sexual behavior patterns, disease history, and hsv type-specific antibody status. the prevalence of hsv-1 antibody was 37.2% in freshmen and 46.1% in fourth-year students; that for hsv-2 antibody was 0.4% and ... | 1990 | 2165103 |
| primary and recurrent herpes simplex virus type 2-induced meningitis. | in 27 patients with a first episode of herpes simplex virus type 2 (hsv-2)-induced meningitis, confirmed by virus isolation from the cerebrospinal fluid (csf) or seroconversion to hsv-2, initial neurologic complications were found in 10 (37%) but subsided before 6 months in all patients. long-term complications were recurrent meningitis in 5 (19%) and periodic headache related to genital hsv recrudescences in 4 (15%). seven additional patients had possible hsv-2-induced recurrent meningitis. in ... | 1990 | 2165105 |
| mucosal and systemic antiviral antibodies in mice inoculated intravaginally with herpes simplex virus type 2. | herpes simplex virus type 2 (hsv-2) causes lethal illness after intravaginal (ivag) inoculation into balb/cj mice. in the present studies, we demonstrated in mice that primary ivag vaccination with an attenuated strain of hsv-2 induced humoral immunity in sera and in vaginal secretions. secondary genital exposure to hsv-2 enhanced this response. however, intraperitoneal exposure to attenuated hsv-2 elicited an antiviral antibody response in sera but not in vaginal secretions. in both sera and va ... | 1990 | 2165134 |
| the jh2604 deletion variant of herpes simplex virus type 2 (hg52) fails to produce necrotizing encephalitis following intracranial inoculation of mice. | the pathological changes and distribution of virus antigen in mouse brains were studied following intracranial inoculation of 3 week old balb/c mice with the herpes simplex virus (hsv) type 2 strain hg52 and its deletion variant jh2604. the variant jh2604 failed to produce necrotizing encephalitis compared to the parental hg52. the morphological changes induced in jh2604-infected brains consisted of localized perivascular cuffing by lymphocytes and infiltration by immune cells. immunohistochemic ... | 1990 | 2165139 |
| alterations in the cellular phenotype induced by herpes simplex viruses. | numerous studies have shown that herpes simplex virus types 1 and 2 (hsv-1, hsv-2) are able to transform the morphological phenotype of rodent cells. unlike other dna tumor viruses the transformed cells did not consistently retain or express a given set of viral genes. in fact, transformation could be obtained using fragments of viral dna that did not wholly encode viral proteins. of interest within the transforming fragments were sequences which could assume a secondary structure like that of i ... | 1990 | 2165516 |
| from virus to vaccine: recombinant mammalian cell lines as substrates for the production of herpes simplex virus vaccines. | the use of mammalian cells as substrates for the production of various vaccines is a time-honored procedure. with the advent of recombinant dna technology, various investigators soon realized that these techniques could be applied to mammalian cells to convert them to reliable, safe producers of a variety of vaccines. while none of these types of recombinant vaccines have, as yet, been widely utilized, many individuals believe that these technologies are the wave of the future in vaccine product ... | 1990 | 2165517 |