control of expression of the herpes simplex virus-induced deoxypyrimidine triphosphatase in cells infected with mutants of herpes simplex virus types 1 and 2 and intertypic recombinants.infection of cells with herpes simplex virus type 1 (hsv-1) induces high levels of deoxypyrimidine triphosphatase. the majority of the enzyme activity is found in infected cell nuclei. a similar activity is induced by hsv type 2 (hsv-2) which, in contrast to the hsv-1 enzyme, fractionates to more than 99% in the soluble cytoplasmic extract. of a series of temperature-sensitive mutants of hsv-1 studied, only the immediate-early mutants in complementation group 1-2 (strain 17 mutants tsd and tsk a ...19826128430
detection of rna complementary to herpes-simplex virus in mononuclear cells from patients with behçet's syndrome and recurrent oral ulcers.viral dna probes were used in in-situ hybridisation to detect the complementary rna in mononuclear cells from the blood of normal subjects and patients with behçet's syndrome and recurrent oral ulcers. hybridisation of 125i-labelled dna probes was detected by means of autoradiography and quantitated with a video image-analysis technique. hybridisation between herpes-simplex virus type 1 (hsv-1) dna and the complementary rna in mononuclear cells was significantly greater in 10 of 20 patients with ...19826129461
induction of nuclear deoxyribopyrimidine triphosphatase and sensitivity of clinical isolates of herpes simplex virus to (e)-5-(2-bromovinyl)-2' a blind study, 21 clinical isolates of herpes simplex virus which had been typed using differential growth on guinea pig embryo versus chicken embryo cells were tested for the presence of the viral deoxyribopyrimidine triphosphatase. in all isolates of type 1, the triphosphatase was present in the nuclei of the infected cells, while none of the hsv-2 isolates induced a nuclear enzyme. in all isolates there was a complete correlation between the presence of nuclear deoxyribopyrimidine triphosp ...19836135391
demonstration of a herpes simplex virus type 2-induced deoxyuridine triphosphate nucleotidohydrolase in infected kb cells and in biochemically transformed hela cells.the deoxyuridine triphosphate nucleotidohydrolase (dutpase) activity which is induced in kb cells infected with herpes simplex virus type 2 (hsv-2) and expressed in biochemically transformed hela cells was separated from the host dutpase activity using either gel filtration or chromatofocusing chromatography. the hsv-2-induced dutpase differed from the host dutpase activity in its molecular weight (53000 versus 46000) and isoelectric point (pi 5 x 9 versus 6 x 4). however, it was similar to the ...19846141216
antiherpes activity of some novel analogues of (e)-5-(2-bromovinyl)-2'-deoxyuridine (e-brvudr) in two different cell a series of 5-vinyl-2'-deoxyuridine (vudr) analogues (5-(2-x-vinyl)-udrs) the (e)-5-(2-bromovinyl)-udr (e-brvudr) proved the most potent inhibitor of plaque formation of two herpes simplex virus type 1 (hsv-1) strains in human embryonic lung fibroblast (helf) and african green monkey kidney (vero) cell cultures. the (z)-5-(2-bromovinyl)-udr (z-brvudr) isomer and the 5-(2,2-dibromovinyl)-udr (br2vudr) analogue were 10-20 times less efficient, whereas the (e)-5-(2-cyanovinyl)-udr (cnvudr) and t ...19846143491
effects of herpes simplex virus strains on human fibroblast and lymphocyte chromosomes and the localization of chromosomal aberrations.the effects on human chromosomes of types 1, 2 and of intermediate serotype of herpes simplex virus (hsv) was compared in fibroblast and lymphocyte cultures. the karyological changes due to hsv were shown to depend on the serotype used as well as on the kind of cells examined (agent-specificity and cell-reaction specificity). differences were noted among the strains in relation to the degree and character of the aberrations induced. conventional giemsa staining and the trypsin g-banding techniqu ...19846145351
differential antiherpes activity of the (e)-and (z)-isomers of 5-(2-fluorovinyl)-2'-deoxyuridine (fvudr).four novel analogues of (e)-5-(2-bromovinyl)-2'-deoxyuridine [(e)-brvudr]--the (e)- and (z)-isomer of 5-(2-fluorovinyl)-udr (fvudr), (z)-5-(2-carboxy-2-fluorovinyl)-udr [(z)-cooh-fvudr], and (e)-5-(2-ethoxyvinyl)-udr [(e)-eovudr] were compared with the reference compounds (e)-brvudr and 5-vinyl-udr (vudr) for their inhibitory effects on plaque formation of herpes simplex virus type 1 (hsv-1 strain 77) and type 2 (hsv-2 strain 82) in human embryonic lung fibroblast (helf) cell cultures. (z)-fvudr ...19846148852
purification of herpes simplex virus-specified major dna-binding proteins and detection of antibodies to them in human and rabbit sera.we purified herpes simplex virus type 1 and 2-specified nonstructural dna-binding proteins (icp8 and icsp 11/12, respectively) from infected vero cells and applied them in elisa test to analyse human and rabbit sera. only a weak immune response to the icp8 and icsp 11/12 could be demonstrated following experimental hsv-1 or hsv-2 infections of rabbits. low levels of icsp8 antibodies were found also in acute hsv infections of man.19846152122
inhibition of herpes simplex virus type 2-induced biochemical transformation by interferon.the effect of interferon on the biochemical transformation of thymidine kinase-deficient cells by uv-inactivated herpes simplex virus type 2 has been studied. transformation was much less sensitive to the action of interferon than virus multiplication. however, the continuous presence of a high dose of interferon (2,000 u) inhibited transformation almost completely. although we could not differentiate between the effect of interferon on fixation and expression of the virus thymidine kinase gene, ...19806154151
biochemical transformation of mouse cells by a purified fragment of marmoset herpesvirus dna.although the size of marmoset herpesvirus (marhv) dna, estimated by velocity sedimentation in sucrose gradients, was similar to that of herpes simplex virus type 1 (hsv-1) dna, the restriction endonuclease sites of marhv and hsv-1 dnas were quite different. a specific bamhi restriction fragment (6.2 x 10(6) daltons) of marhv dna biochemically transformed lm(tk-) mouse fibroblasts to the thymidine kinase(tk)-positive phenotype. rabbit antisera, prepared against marhv tk, inhibited marhv-induced t ...19806154671
structural analysis of the capsid polypeptides of herpes simplex virus types 1 and 2.capsids of herpes simplex virus (hsv) types 1 and 2 contain seven polypeptides ranging in molecular weight from 154,000 to 12,000 (termed nc-1 through nc-7 in order of descending molecular weight). antibodies prepared to hsv-1 capsid polypeptides isolated from sodium dodecyl sulfate-polyacrylamide gels reacted in an immunofluorescence assay against hsv-1-infected kb cells. three of the antibodies (anti-nc-1, anti-nc-2, and anti-nc-3,4) also reacted with hsv-2-infected cells. tryptic peptide anal ...19806154808
glycoproteins with type common and type specific antigenic sites excreted from cells infected with herpes simplex virus types 1 and 2.agar immunodiffusion tests demonstrated that bhk 21 cells infected with either hsv i or hsv 2 release only a few hsv-specified antigens into the extracellular fluid (infected cell released polypeptides-icrp). neutralization blocking experiments showed that the majority of antigens/(including the band ii common antigen) involved as target sites in antibody-mediated virus neutralization are present in the icrp of both hsv i and hsv 2.sds-page identified six regions of virus-specified proteins in t ...19806156995
function, target-cell preference and cell-surface characteristics of herpes-simplex virus type-2-induced non-antigen-specific killer cells.wild-type and congenitally athymic nude mice injected with herpes-simplex virus type 2 (hsv 2) responded with a local outburst of non-antigen-specific killer cells masking any virus-specific response. cytolytic activity could be assayed on mouse-tumor cell lines and on syngeneic or allogeneic non-transformed cells from various sources. some of the tumor cell lines and proteose-peptone-induced peritoneal exudate cells were lysed more efficiently after infection with either hsv 2, vaccinia or infl ...19816165679
comparison of the effects of arabinosyladenine, arabinosylhypoxanthine, and arabinosyladenine 5'-monophosphate against herpes simplex virus, varicella-zoster virus, and cytomegalovirus with their effects on cellular deoxyribonucleic acid a single line of human foreskin fibroblasts, minimum inhibitory concentrations (mics) and the minimum intracellular virus inactivation concentrations (miics) of arabinosyladenine, arabinosylhypoxanthine, and arabinosyladenine 5'-monophosphate were assayed for a number of recent isolates of herpes simplex virus types 1 and 2 (hsv-1, hsv-2), varicella-zoster virus (vzv), and cytomegalovirus (cmv). (the term miic is used here to describe the selective qualitative intracellular inhibition of the ...19816166244
detection of rna complementary to herpes simplex virus dna in human cervical squamous cell neoplasms.nonneoplastic and neoplastic cervical biopsy specimens were examined by in situ hybridization to 125i-labeled dna of herpes simplex virus (hsv), adenovirus, and bacteriophage lambda dna's, and quantitative hybridization data were obtained using a video image analyser. hsv-specific rna was detected in 72% of cervical intraepithelial neoplasia, 60% of squamous cervical carcinomas, 2% of nonneoplastic cervices, and 9% of primary adenocarcinomas of the cervix. none of the tissues gave positive hybri ...19816167349
monoclonal antibodies to two glycoproteins of herpes simplex virus type 2.monoclonal antibodies to herpes simplex virus type 2 were found to precipitate different numbers of radiolabeled polypeptides from lysates of virus-infected cells. antibodies directed against two viral glycoproteins were characterized. antibodies from hybridoma 17 alpha a2 precipitated a 60,000-molecular-weight polypeptide which chased into a 66,000- and 79,000-molecular-weight polypeptide. all three polypeptides labeled in the presence of [3h]glucosamine and had similar tryptic digest maps. the ...19816168775
characteristics of a persistent rubella infection in a human cell line.a persistent infection of the human mcf-7 cell line (mcf-7rv) was established with the dbs strain of rubella virus at a low multiplicity of infections. fluorescent antibody staining revealed that 100% of the cells were positive for rubella antigens. the infected cells were refractory to superinfection with vesicular stomatitis virus (vsv) but were susceptible to herpes simplex virus type 2 (hsv-2). no interferon could be detected in infected cell culture fluid, and continuous passage in the pres ...19816169785
herpes simplex virus type 1 and 2 intracellular p40: type-specific and cross-reactive antigenic determinants on peptides generated by partial proteolysis.intracellular p40 is a class of protein ranging in molecular weight from 39,000 to 45,000 that is immunoprecipitated from herpes simplex virus type 1 (hsv-1)- and hsv-2-infected cell extracts by mouse monoclonal antibodies or guinea pig antisera against hsv-1 and hsv-2 nucleocapsid p40. analysis by a two-dimensional gel system showed that hsv-1 and hsv-2 intracellular p40 each consisted of three major components. however, these hsv-1 and hsv-2 proteins differed in charge and size. analysis of st ...19816172597
effect of monoclonal antibodies on limited proteolysis of native glycoprotein gd of herpes simplex virus type 1.we examined the properties of 17 monoclonal antibodies to glycoprotein gd of herpes simplex type 1 (hsv-1) (gd-1) and hsv-2 (gd-2). the antibodies recognized eight separate determinants of gd, based on differences in radioimmuno-precipitation and neutralization assays. the determinants were distributed as follows: three were gd-1 specific, one was gd-2 specific, and four were type common. several type-specific and type-common determinants appeared to be involved in neutralization. we developed a ...19826176725
protease inhibitors suppress fibrinolytic activity of herpesvirus-transformed cells.previous studies in this laboratory have quantified the fibrinolytic activity of herpesvirus-transformed cell lines and implicated the proteolytic capacity of cloned cell lines in the formation of primary and metastatic tumours. because of the involvement of proteases in tumourigenesis in this system, we examined the effect of various protease inhibitors (alpha-1-antitrypsin, leupeptin and alpha-2-macroglobulin), as well as hamster serum containing acute phase proteins generated in response to p ...19826178796
anti-herpesvirus activity of adenine arabinoside analogues in tissue culture and a genital infection of mice and guinea pigs.four analogues of adenine arabinoside (ara-a) were compared for activity against herpes simplex virus (hsv) in tissue culture and in a genital infection of mice and guinea pigs. these analogues, 5'-monophosphate (ara-amp), 5'-valerate ester (ara-av), 2'3'-diacetate ester (ara-ada), and 2',3',5'- triacetate ester (ara-ata) have greater water and lipid solubility and resistance to deamination than ara-a. in mouse embryo fibroblast cells, similar viral inhibitory levels were noted with ara-a, amp, ...19826179469
effect of combinations of acyclovir with vidarabine or its 5'-monophosphate on herpes simplex viruses in cell culture and in mice.the combination of various concentrations of acyclovir and vidarabine or its 5'-monophosphate usually produced an additive interaction with various strains of herpes simplex virus types 1 and 2 in vero cells. similarly, certain combinations of these drugs were more effective than the individual drugs in decreasing the mortality and increasing the mean day of death of mice inoculated intracerebrally with herpes simplex virus type 2. neither antagonism nor interference was noted for any of the in ...19826182836
herpes simplex infection simulating a positive auto-inoculation for haemophilus from a genital ulcer suspected of being ulcus molle gave redness after 24 hours and after 48 hours vesicles and pustules appeared. cultivation from the auto-inoculation after the 48 hours was positive for herpes simplex virus type 2. our observation underlines two points: auto-inoculation for the diagnosing of haemophilus ducreyi infection may be mimicked by herpes simplex infection, and the incubation period of herpes simplex can be shorter than the 4-5 days usually given.19826183910
experimental infection of inbred mice with herpes simplex virus. iv. comparison of interferon production and natural killer cell activity in susceptible and resistant adult mice.inbred mouse strains differ in susceptibility to infection with herpes simplex virus type 1 or type 2 (hsv-1, hsv-2). in this study interferon production was tested in the peritoneal exudate of mice after intraperitoneal (i.p.) injection of hsv-1 or hsv-2. in hsv-resistant mice (c57 bl/6, c3 h/hej) high titers of interferon were already present 2 to 4 hours after injection. in comparison, less resistant mice (dba/2, akr) lacked this early response. there was no correlation between interferon tit ...19826187314
hla-restricted t lymphocyte-mediated cytotoxicity against herpes simplex virus-infected cells in humans.cytotoxic t lymphocytes (ctl) against herpes simplex virus (hsv) were induced in vitro from human peripheral blood lymphocytes by stimulation with hsv antigen. ctl generated by hsv type 1 (hsv-1) antigen stimulation killed not only hsv-1-infected target cells but also hsv type 2 (hsv-2)-infected target cells, though at a lower level. this evidence suggests that ctl against hsv recognize the hsv type-specific and type-common determinants on hsv-infected target cells. these ctl were generated from ...19836187690
characteristics of a shared idiotype by two igm anti-herpes simplex virus monoclonal antibodies that recognize different determinants.two igm monoclonal antibodies (mcab) that recognized distinct antigenic determinants of herpes simplex virus type 2 (hsv2) were shown to share idiotypes. seven other igm mcab generated to either hsv2 or the major envelope glycoprotein of hsv2, vp119, did not express this shared idiotype. the idiotypic determinants were associated with the antibody-combining site, because hsv2 inhibited the idiotype-anti-idiotype reactions. anti-hsv2 sera raised in balb/c mice also expressed the idiotype shared b ...19836187827
antigenic variants of herpes simplex virus selected with glycoprotein-specific monoclonal antibodies.monoclonal antibodies specific for herpes simplex virus type 1 (hsv-1) glycoproteins were used to demonstrate that hsv undergoes mutagen-induced and spontaneous antigenic variation. hybridomas were produced by polyethylene glycol-mediated fusion of p3-x63-ag8.653 myeloma cells with spleen cells from balb/c mice infected with hsv-1 (strain kos). hybrid clones were screened for production of hsv-specific neutralizing antibody. the glycoprotein specificities of the antibodies were determined by sod ...19836187935
human cytomegalovirus and herpes simplex type 2 virus in normal and adenocarcinomatous prostate glands.normal prostate, benign prostate hypertrophy (bhp), and prostate adenocarcinoma (acp) biopsy specimens were analyzed for the presence of human cytomegalovirus (hcmv) and herpes simplex virus type 2 (hsv-2)-specific macromolecules. hcmv dna homologous sequences were detected in 2 of 13 normal prostate, 2 of 9 bhp, and 3 of 10 acp tissues, and hsv-2 dna homologous sequences were found in 1 normal prostate tissue and 2 acp tissues. in situ dna-rna hybridizations indicated hcmv-specific rna in 3 of ...19836188872
a micro solid-phase radioimmunoassay for detection of herpesvirus type-specific antibody: parameters involved in standardization.a micro solid-phase radioimmunoassay (micro-spria) was developed to demonstrate type-specific antibodies to herpes simplex virus types 1 and 2 (hsv1 and hsv2). glycoproteins from the 123,000 dalton region of hsv1 (vp123) and the 119,000 dalton region of hsv2 (vp119) were isolated on preparative polyacrylamide gels for use as antigens in the micro-spria. human sera selected from clinical samples by virological history and appropriate microneutralization data were used to standardize the micro-spr ...19836189848
a micro solid-phase radioimmunoassay for detection of herpesvirus type-specific antibody: specificity and sensitivity.the specificity and sensitivity of a micro solid-phase radioimmunoassay (micro-spria) that detects type-specific igg antibody to herpes simplex virus types 1 and 2 (hsv1 and hsv2) were evaluated. glycoproteins vp123 (molecular weight, 123,000) of hsv1 and vp119 (molecular weight, 119,000) of hsv2 were found to display the greatest degree of antigenic type-specificity of several hsv antigens tested with the micro-spria technique. when testing a group of sera, negative for anti-hsv antibodies by m ...19836189849
rapid and selective shutoff of plasma protein production in herpes simplex virus type 2-infected hepatoma cells.the effect of herpes simplex virus type 2 (hsv-2) infection of hepatoma mca-rh7777 cells on the production of alpha-fetoprotein (afp) and several other secreted plasma proteins was examined. cells infected with hsv-2 were labeled with [35s]methionine for various times postinfection (p.i.). culture media and intracellular extracts were immunoprecipitated with plasma protein antibodies and examined by polyacrylamide gel electrophoresis. the relative levels of afp and several other plasma proteins ...19836190309
herpes simplex virus type 2 glycoprotein gf and type 1 glycoprotein gc have related antigenic determinants.the 104-s monoclonal antibody immunoprecipitated from herpes simplex virus type 2 (hsv-2)-infected cell extracts the 75,000-molecular-weight glycoprotein gf and its 65,000-molecular-weight precursor (pgf). the precursor pgf was sensitive to endoglycosidase h digestion, indicating the presence of high mannose-type oligosaccharides, whereas the stable gf product was sensitive to neuraminidase digestion, indicating the presence of sialic acid residues. the 104-s antibody also weakly precipitated th ...19836191040
characterization of a herpes simplex virus type 2 75,000-molecular-weight glycoprotein antigenically related to herpes simplex virus type 1 glycoprotein c.evidence is presented that the herpes simplex virus type 2 glycoprotein previously designated gf is antigenically related to herpes simplex virus type 1 gc (gc-1). an antiserum prepared against type 1 virion envelope proteins immunoprecipitated gf of type 2 (gf-2), and competition experiments revealed that the anti-gc-1 component of the antiserum was responsible for the anti-gf-2 cross-reactivity. an antiserum prepared against fully denatured purified gf-2, however, and three anti-gf-2 monoclona ...19836194310
mode of protection of mice against herpes simplex virus type 2 infection by propionibacterium.we compared various strains of propionibacterium with regard to protection of young adult mice against lethal infection with herpes simplex virus type 2 (hsv-2). propionibacterium acnes, p. granulosum, and p. avidum were protective, while p. acidi-propionici and p. lymphophilum were ineffective. the protective effect proved to be in the cell wall fraction. attempts were made to elucidate possible mechanisms of the protection using both effective and ineffective strains. the results strongly sugg ...19836195512
x-linked resistance of mice to high doses of herpes simplex virus type 2 correlates with early interferon production.mice inoculated intraperitoneally with herpes simplex virus type 2 develop focal necrotizing hepatitis and eventually die from ascending myelitis and encephalitis. the genetics of resistance to the infection were analyzed in crosses between resistant c57bl/10 mice and susceptible balb/c mice. it was shown that the resistance of c57bl/10 mice to hepatitis induction was influenced by an x-linked dominant gene as previously shown for the gr mouse strain. the course of infection in the liver pointed ...19836196293
comparison of the antiviral effects of 5-methoxymethyldeoxyuridine-5'-monophosphate with adenine arabinoside-5'-monophosphate.methoxymethyldeoxyuridine-5'-monophosphate (mmudr-mp) and arabinofuranosyladenine-5'-monophosphate (ara-amp) had significant antiviral activity against herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) in rk-13 and vero cells. mmudr-mp and ara-amp were more potent than methoxymethyldeoxyuridine (mmudr) and arabinofuranosyladenine (ara-a) against the ms strain of hsv-2. mmudr-mp inhibited replication of hsv-1r (mutant resistant to mmudr). mmudr in combination with ara-amp showed additive act ...19836197027
localization and synthesis of an antigenic determinant of herpes simplex virus glycoprotein d that stimulates the production of neutralizing antigenic determinant capable of inducing type-common herpes simplex virus (hsv)-neutralizing antibodies has been located on glycoprotein d (gd) of hsv type 1 (hsv-1). a peptide of 16 amino acids corresponding to residues 8 to 23 of the mature glycoprotein (residues 33 to 48 of the predicted gd-1 sequence) was synthesized. this peptide reacted with an anti-gd monoclonal antibody (group vii) previously shown to neutralize the infectivity of hsv-1 and hsv-2. the peptide was also recognized by p ...19846197535
identification and preliminary mapping with monoclonal antibodies of a herpes simplex virus 2 glycoprotein lacking a known type 1 counterpart.the properties of herpes simplex virus 2 (hsv-2)-specific proteins reactive with monoclonal antibody h966 derived from mice immunized with hsv-2 strain g are reported. the reactive proteins contained in infected cell lysates subjected to electrophoresis in denaturing gels and transferred to nitrocellulose sheets form a relatively sharp band characteristic of mr 124,000 proteins and a diffuse, more slowly migrating band. antigens reactive with h966 were detected on the surface of viable, unfixed ...19846199891
a rapid procedure for the enrichment of undenaturated, antigenically active herpes simplex virus glycoproteins.the usefulness of lentil lectin affinity chromatography for the rapid enrichment of hsv glycoproteins in an undenatured state for both research and clinical purposes was investigated. in order to compare the lentil lectin-binding characteristics and immunologic specificities of undenatured hsv-1 and hsv-2 glycoproteins, [35s]methionine-labelled extracts of virus-infected hep-2 cells were subjected to lentil lectin affinity chromatography. individual hsv-1 and hsv-2 glycoproteins in bound and unb ...19846200489
potent synergistic inhibition of herpes simplex virus-2 by 9-[(1, 3-dihydroxy-2-propoxy)methyl]guanine in combination with recombinant interferons.dhpg, an acyclic guanine nucleoside with the structure 9-[(1,3-dihydroxy-2-propoxy)methyl]guanine], showed potent synergism with recombinant alpha or beta interferons and modest synergism with gamma interferon in inhibiting the replication of herpes simplex virus type 2 in vitro. the most potent direct anti-herpes viral synergism was obtained by combination of dhpg and recombinant human interferon-beta-ser17; when combined, doses of each near their separate effective dose50's resulted in almost ...19846201172
successful use of oligopeptides as immunogens in the preparation of antisera to immediate-early gene products of herpes simplex virus type 1.antisera to two herpes simplex virus-type 1 (hsv-1) immediate-early gene products ( ie12 and ie175 ) have been produced using oligopeptides, constructed on the basis of proposed dna coding sequences, as immunogens. in both cases the synthetic peptides were linked to bovine serum albumin via an n-terminal tyrosine prior to immunization. both the ie12 and the ie175 antisera reacted with their respective hsv-1 antigens and with antigens produced by the hsv-1 immediate-early mutant tsk but not with ...19846202828
antiidiotype modulation of herpes simplex virus infection leading to increased pathogenicity.antiidiotype reagents that recognize idiotypic determinants associated with the combining site of monoclonal antibodies to herpes simplex virus type 2 ( hsv2 ) were used to manipulate the immune response to hsv2 in balb/c mice. the injection of antiidiotype antibodies into mice before challenge with a 50% lethal dose of hsv2 resulted in a shorter survival time than that of mice receiving either preimmune rabbit immunoglobulin g or antiidiotype reagents against hepatitis b surface antigen before ...19846202879
localization of a type-specific antigenic site on herpes simplex virus type 2 glycoprotein d.a herpes simplex virus type 1 strain isolated from a recurrent lesion of the nose reacted with monoclonal antibodies recognizing a type 2-specific site on glycoprotein d but not with monoclonal antibodies recognizing other type 2-specific sites. dna sequence analysis of the glycoprotein d gene of the isolate revealed a single nucleotide alteration which changed the codon for asparagine to one encoding histidine at amino acid 97 in the protein. histidine is located at this position in glycoprotei ...19846202883
inhibition of the herpes simplex virus-coded thymidine kinase-complex by 9-beta-d-arabinofuranosyladenine 5'-monophosphate (ara-amp) and 9-(2-hydroxyethoxymethyl)guanine-monophosphate (acyclo-gmp).the thymidine kinase-complex isolated from herpes simplex virus type 1 and type 2 (hsv-1 and hsv-2) is associated with the following enzyme activities:atp:dthd (dcyd) deoxypyrimidine kinase, atp:dtmp thymidylate kinase, adp:dthd- and amp:dthd 5'-phosphotransferase. in kinetic experiments it is shown that ara-amp inhibits amp:dthd- and adp:dthd phosphotransferase activity, while acyclo-gmp impairs adp:dthd phosphotransferase reaction only; the inhibition was found to be non-competitive. the funct ...19846206825
human cytotoxic t cell clones directed against herpes simplex virus-infected cells. ii. bifunctional clones with cytotoxic and virus-induced proliferative activities exhibit herpes simplex virus type 1 and 2 specific or type common reactivities.human cytotoxic t lymphocyte (ctl) clones directed against herpes simplex virus (hsv)-infected cells were generated after stimulation of peripheral blood lymphocytes (pbl) with hsv type 1 (hsv-1) and hsv type 2 (hsv-2). these ctl clones were studied with regard to hsv type specificity and with regard to whether they also express helper cell activity. some clones, generated after stimulation with hsv-1, were cytotoxic for autologous cells infected with either hsv-1 or hsv-2 ("hsv type common clon ...19846207243
characterization of mrnas that map in the bglii n fragment of the herpes simplex virus type 2 genome.the bglii n dna fragment (0.580 to 0.620 map units) of herpes simplex virus type 2 strain (333) is of interest because of its transforming potential. this fragment contains either partial or the complete coding sequences for nine mrna transcripts that can be detected during a lytic infection. subclones of the bglii n dna fragment were generated in plasmid vectors, and the approximate locations of the mrna transcripts were mapped by rna blot hybridization technology. precise 5' or 3' ends (or bot ...19846207312
sensitivity and specificity of monoclonal antibodies directed against antigenic determinants of treponema pallidum nichols in the diagnosis of syphilis.murine anti-treponema pallidum monoclonal antibodies were employed in studies on sensitivity and specificity of binding to examine their potential for use in the detection of low numbers of pathogenic treponemes present in various body fluids. monoclonal antibodies were used as a primary antibody source in a solid-phase immunoblot assay system. all monoclonal antibodies assayed were capable of detecting ca. 1.0 x 10(3) to 2.5 x 10(3) treponemes. of 13 monoclonal antibodies examined, 3 were able ...19846208218
fine structure analysis of type-specific and type-common antigenic sites of herpes simplex virus glycoprotein d.the fine structure of the antigenic determinants of herpes simplex virus type 1 and 2 glycoprotein d (gd) was analyzed to determine whether structural differences underlie the differential immunogenicity of these glycoproteins. a region common to herpes simplex virus type 1 and 2 gd (amino acid residues 11 to 19) and two sites specific for herpes simplex virus type 2 gd (one determined by proline at position 7, the other determined by asparagine at position 21) were localized within the n-termin ...19846208376
immunity to herpes simplex virus type 2. v. risk of recurrent disease following primary infection: modulation of t-cell subsets and lymphokine (lif) production.phenotypic and functional characteristics of peripheral blood lymphocytes (pbl) from 133 patients (87 female; 46 male) with documented primary or recurrent genital hsv-2 infection were determined by serologic testing and cmi [lymphocyte blastogenesis and lymphokine (lf)] evaluation. primary infection was followed by the development of virus-specific immune memory as defined in vitro by lymphocyte blastogenesis and the development of neutralizing antibody. virus-specific lif production was detect ...19836209056
clonal analysis of t-cell responses to herpes simplex virus: isolation, characterization and antiviral properties of an antigen-specific helper t-cell clone.a herpes simplex virus (hsv)-specific long-term t-cell clone has been established from the draining lymph node cells of balb/c mice; the cells required repeated in vitro restimulation with uv-irradiated virus. the established t-cell clone expresses the thy-1 and lyt-1+2,3- surface antigens. for optimal proliferation of the cloned cells, both the presence of specific antigen and an exogenous source of t-cell growth factor are required. the proliferative response of the cloned t cells was found to ...19846209206
immunofluorescent evidence of prior herpes simplex virus type-2 infection in prostate carcinoma.the finding of herpes simplex virus type-2 (hsv-2) particles in prostatic carcinoma (pca) tissue has led to speculation that the virus might cause this disease. we studied 27 pca and 33 benign prostatic hyperplasia (bph) specimens for the presence of hsv-2 antigens by indirect immunofluorescent staining to hsv-2 using commercially prepared rabbit anti-hsv-2 and fluorescein-tagged goat and anti-rabbit antibody. the slides were randomly number coded by an impartial referee then read independently ...19846209848
changes in the vaginal cytology of the guinea pig induced by herpes simplex virus.the vaginal cytology of guinea pigs with genital herpes simplex virus type 2 (hsv-2) infection was examined during acute and latent infection. cytologic changes similar to those observed in humans with hsv-2 genital infections were observed in the guinea pigs during acute infection. during latent infection, some animals continued to have abnormal cytology. the severity of the changes was reduced after treatment with phosphonoformic acid during acute infection.19836223477
immunity to herpes simplex virus type 2: recurrent lesions are associated with the induction of suppressor cells and soluble suppressor factors.cell-mediated immunity to herpes simplex virus type 2 was investigated in infected inbred strain 13/n guinea pigs with (45%), and without, a history of recurrent herpetic disease (a. d. donnenberg, e. chaikof, and l. aurelian, infect. immun. 30:99-109, 1980). induction of suppressor cells capable of reducing the proliferative response of herpes simplex virus type 2-stimulated immune lymphoid cells was demonstrated in spleen cells from animals with a history of recurrent disease at recrudescence ...19836227561
treatment of primary acute genital herpes in guinea pigs by intraperitoneal administration of fluoropyrimidines.fiac [1-(2'-deoxy-2'-fluoro-beta-d-arabinofuranosyl)-5-iodocytosine], fiau [1-(2'-deoxy-2'-fluoro-beta-d-arabinofuranosyl)-5-iodouracil], and fmau [1-(2'-deoxy-2'-fluoro-beta-d-arabinofuranosyl)-5-methyluracil] were evaluated for their efficacies in the treatment of genital infections with herpes simplex virus type 2 in guinea pigs. intraperitoneal administration of these drugs in daily doses of 100 mg/kg of body weight initiated 24 h after virus inoculation and repeated 2 successive days therea ...19846239591
comparison of foldback sequences of herpes simplex virus types 1 and 2 dna.the dnas of herpes simplex virus types 1 and 2 (hsv-1 and hsv-2) were separately denatured and allowed to renature briefly. the intrastand foldback structures that resulted from base pairing of inverted repeated sequences on otherwise single-stranded (ss) dna were visualized in the electron microscope. the two genomes were found to contain similar size classes of small duplex stem dna sequences. however, hsv-2 dna appeared to possess an additional, larger size class of foldback structures not fo ...19806243346
association of herpes simplex thymidine kinase gene with chromosome no. 18 in transformed human cells.the herpes simplex virus type-2 (hsv-2)-transformed human cell line hb-2-3 was fused with thymidine kinase (tk)-deficient mouse cells [lm(tk-)], and 12 independent hybrids were isolated with the use of the hat (hypoxanthine, amethopterin, and thymidine)-ouabain selection system. discontinuous polyacrylamide gel electrophoresis studies demonstrated that the hsv-2-specific tk was the selected enzyme in the hybrids. isoenzyme analysis and karyotyping were used in the analysis of the hybrids for the ...19806243715
suppression by progesterone of nonspecific in vitro lymphocyte stimulation in mice as a mechanism for the enhancement of herpes simplex virus type 2 vaginal infection.the role of antibody, interferon, and cell-mediated immunity (cmi) were studied to determine the mechanisms for progesterone enhancement of vaginal herpes simplex virus type 2 (hsv 2) infection in mice. three groups of mice were studied: nonpregnant control, pregnant, and nonpregnant progesterone-treated mice. vaginal infection with hsv 2 did not elicit a neutralizing antibody or a systemic interferon response in any of the groups tested. splenic lymphocytes from noninfected and infected mice we ...19806243861
treatment of genital herpes simplex virus in male patients.twenty-three male patients with herpes simplex virus type 2 blisters on the prepuce, glans penis, and penile shaft were divided into 2 groups. ten patients served as controls, and thirteen patients were treated with ultrasound (1 w/cm2 for 60 sec) and herpigon on 3 consecutive days. results showed a negative virus culture after 3 days of treatment and significant reduction of the recurrent infection in the treated group while the control group experienced recurrent infection within 62--80 days. ...19806243914
characterization of herpes simplex virus strains isolated from patients with various diseases.herpes simplex virus strains (hsv) were isolated from various herpetic diseases. these hsv-strains isolated in bulgaria (687) and in other countries (13) were studied by the neutralization test using standard type specific rabbit antisera and human gamma globulin. the serotype distribution of all strains showed: hsv-1 = 323 strains, hsv-2 = 372 strains, 5 strains behaved antigenically intermediate. a close correlation between the serotype of the strains and the localization of the lesions was es ...19806243918
different susceptibilities of skin to type 1 and type 2 herpes simplex viruses in newborn infections with type 1 herpes simplex virus (hsv-1) were compared with skin infections with type 2 virus (hsv-2). five strains each of hsv-1 and hsv-2 were tested by injecting 10(3) 50% tissue culture infective doses of each strain subcutaneously into 1-day-old new zealand white rabbits. all five strains of hsv- 2 produced severe skin lesions that resulted in wide dissemination of the infection to many organs, paralysis of the hind legs, and finally death. the virus could be isolated freque ...19806244227
distribution of sequences homologous to the dna of herpes simplex virus, types 1 and 2, in the genome of pseudorabies virus.the distribution of related sequences between the genomes of pseudorabies virus (prv) and herpes simplex virus, types 1 (hsv-1) and 2 (hsv-2), was determined. approximately 7% of the sequences in prv are shared by hsv-1 and hsv-2 dnas. by means of the southern blot technique, it was found that the homologous sequences are not sequestered in one region but are distributed throughout the prv genome. hsv-1 and hsv-2 have the greatest homology with the long unique region of prv dna and the least wit ...19806244240
reduction of cell surface fibronectin (lets protein) correlates with tumorigenicity of hamster fibroblasts transformed by herpes simplex virus type2.a series of herpes simplex virus type 2 (hsv-2)-transformed hamster cells has been examined using the technique of lactoperoxidase-catalyzed cell surface protein iodination. the amount of cell surface fibronectin (lets protein) was reduced after hsv-2 transformation, and this reduction correlated with increased tumor-forming ability.19806244242
a type-specific antiserum induced bya major herpesvirus type 1 glycoprotein.a type-specific antiserum was prepared against vp 7/8, the major hsv-1 type-specific glycoprotein. the specificity of this antiserum was demonstrated by both neutralization of hsv-1 infectivity and immunoprecipitation followed by sds-polyacrylamide gel electrophoresis of the immunoprecipitates. only minimal cross-reactivity with hsv-2 was observed. this major vp 7/8 glycorprotein was also purified from a host of virus-induced proteins with the specific immunoadsorbent prepared with anti-vp 7/8 s ...19806244352
analysis of viral dna sequences in hamster cells transformed by herpes simplex virus type 2.herpes simplex virus type 2 (hsv-2) dna was treated with four restriction endonucleases (ecori, hinciii, bgl ii, and xba i) and eight fragments were purified and labeled with 32p in vitro. the kinetics of renaturation of each of the fragments was measured in the presence of dna extracted from 333-8-9, a hamster cell line transformed by uv light-inactivated hsv-2 strain 333, and from a series of cloned derivatives and their tumor lines. all of the lines examined contained a partial set of viral s ...19806244590
cytomegalovirus and dual infection in four unrelated infants without underlying immunodeficiency, dual infections with cytomegalovirus (cmv) and another microorganism developed. the patients included the following: (1) a 3-month-old girl with congenital cmv and perinatal cutaneous herpes simplex virus; (2) a 5-week-old girl with cmv and pneumocystis carinii; (3) a 12-week-old girl with cmv and haemophilus influenzae meningitis; and, (4) a 2 1/2-month-old girl with cmv and escherichia coli meningitis. in all four cases, the patien ...19806244804
some characteristics of an early protein (icp 22) synthesized in cells infected with herpes simplex vero cells incubated at 40 degrees c or treated with azetidine at 37 degrees c, synthesis of a polypeptide ('c') of apparent mol. wt. 66000 was stimulated. it was not phosphorylated and was found in the cytoplasmic fraction of cell lysates. in cells infected with herpes simplex virus type 1 (hsv-1) in the presence of azetidine, synthesis of cellular proteins, including polypeptide c, was suppressed and infected cell polypeptides icp 4, 0, 22 and 27 (apparent mol. wt. 170000, 120000, 75000 and ...19806245173
identification of the herpes simplex virus dna sequences present in six herpes simplex virus thymidine kinase-transformed mouse cell lines.we have used a novel filter hybridization approach to detect and map the herpes simplex virus (hsv) dna sequences which are present in four hsv thymidine kinase (hsvtk+)-transformed cell lines which were derived by exposure of thymidine kinase negative (tk-) mouse cells to uv light-irradiated hsv type 2 (hsv-2). in addition, we have mapped the hsv-1 dna sequences which are present in two hsv-1tk+-transformed cell lines produced by transfection of tk- mouse cells with sheared hsv-1 dna. the resul ...19806245232
location and cloning of the herpes simplex virus type 2 thymidine kinase gene.the herpes simplex virus type 2 thymidine kinase gene has been mapped to a position colinear with the herpes simplex virus type 1 thymidine kinase gene and cloned within a 4.0-kilobase fragment in pbr 322.19806245273
specific viral antigens in rat cells transformed by herpes simplex virus type 2 and in rat tumors induced by inoculation of transformed cells.a rat cell line, re1, oncogenically transformed by a herpes simplex virus type 2 ts mutant (ts 1), has been demonstrated to contain herpes simplex virus type 2-specific thymidine kinase activity, as have two of four tumors induced in rats by inoculation of these transformed cells. a high proportion of sera from tumor-bearing rats (5 of 11) have detectable antibody against herpes simplex virus thymidine kinase, and there is a correlation between enzyme activity in a tumor and antibody in "tumor s ...19806245808
experimental type 2 herpes simplex ophthalmitis in the newborn animal model for the study of type 2 herpes simplex virus (hsv2) ophthalmitis is described. wistar rats were inoculated intracerebrally with hsv2 at 0, 5, 10, 15, 20, or 30 days of age. eyes and brain from all animals, whether they survived or succumbed with encephalitis, were collected for microscopic and virologic studies. up to 20% or more of the hsv2-inoculated rats had lesions in the cornea, uveal tract, and/or retina. herpetic keratiis occurred in a few animals while the eyelids were st ...19806246021
tubular structures in the cervix of mice experimentally infected with herpes simplex virus type 2.experimental infection of the c3h/n mouse genital tract was demonstrated after intravaginal inoculation with herpes simplex virus type 2 (hsv-2). about 75% of the infected animals died by day 7, and 75% of the surviving animals had severe vaginitis or neurological signs on day 7. titers of the virus recovered from vaginal secretions of infected animals reached a maximum on day 2 and gradually decreased until day 7. on the other hand, under the electron microscope, virus particles and tubular str ...19806246195
resistance of herpes simplex virus to acycloguanosine: role of viral thymidine kinase and dna polymerase loci.acycloguanosine [9-(2-hydroxyethoxymethyl)guanine; acyclo-guo] is a potent inhibitor of herpes simplex viruses (hsv); it is selectively phosphorylated in virus-infected cells. in order to define those viral functions that may mediate resistance to acyclo-guo, the drug sensitivities of temperature-sensitive (ts) and phosphonoacetic acetic acid (paa)-resistant mutants of hsv-1 and hsv-2 have been determined. two distinct viral genetic loci are independently associated with acyclo-guo resistance. m ...19806246532
tumorigenic transformation induced by a specific fragment of dna from herpes simplex virus type 2.transfection of syrian hamster embryo cells with limit digests of bgl ii-, hpa i-, or bgl ii/hpa i-cleaved dna from herpes simplex virus type 2 (strain s-1) but not with salmon sperm dna resulted in the appearance of refractile, morphologically altered cells at a frequency of 10(-5)/0.005 microgram of viral dna within two to four passages. transformed lines manifested reduced serum requirement and anchorage-independent growth and were tumorigenic in newborn hamsters. they expressed icp10, a vira ...19806246534
herpes simplex virus infection: problems and prospects as perceived by a peripatetic pediatrician.the multivaried aspects of the herpes simplex viruses (hsv) types 1 and 2 and the infections they produce are discussed. points emphasized are: (1) the need for considering these (and other viruses) from an evolutionary perspective; (2) the necessity of disseminating current methods for virus identification; (3) the great progress in molecular-virological aspects and in the genetics of the virus which provide new tools for epidemiological and immunological studies and define more convincingly th ...19806246686
comparative analysis of the virion polypeptides specified by herpes simplex virus type 2 strains.this paper reports on the variability of structural polypeptides of 32 strains of herpes simplex virus 2 (hsv-2) isolated from various locations in the united states and italy. most strains were passaged a limited number of times at low multiplicity outside the human host; a few strains were characterized by numerous passages at variable multiplicities in cell culture. the acrylamide gel electrophoresis of polypeptides from purified virions revealed very few differences. considering these differ ...19806246854
collagenase levels in a new model of experimental herpetic interstitial keratitis.a reproducible model of severe herpetic interstitial keratitis was developed by injecting live herpes simplex virus type 2 intrastromally into the corneas of presensitized rabbits. herpes-infected corneas showed significantly more stromal infiltration and vascularization, iritis, conjunctivitis, and epithelial disease than the control corneas injected with cell supernatant without virus. levels of total collagenase detected in the culture media of the herpes-infected corneas were high and were s ...19806246865
macrophage extrinsic antiviral activity during herpes simplex virus infection.peritoneal macrophages from mice infected with herpes simplex virus type 2 (hsv-2) exhibited extrinsic antiviral resistance. when the macrophages were co-cultivated in vitro with virus-infected cells the yield of virus was reduced markedly. activity was not present 1 to 2 days p.i., peaked at 3 to 4 days, declined by 7 days and was absent at 14 days after hsv-2 infection. the extrinsic antiviral activity was limited to the adherent peritoneal macrophage population. the macrophage antiviral activ ...19806247426
comparative neurovirulence and latency of hsv1 and hsv2 following footpad inoculation in mice.the effect of virus dose and animal age on the appearance of acute and latent neurologic infection by hsv1 and hsv2 was studied in balb/c and icr mice inoculated in the footpad. at low viral doses hsv2 was found to be 1,500 times more neurovirulent than hsv1. at high doses there was no difference in neurovirulence. age-acquired resistance to disease was shown to be less complete with hsv2 than with hsv1. neurovirulence was shown to be associated with spread of infection to the spinal ganglia. th ...19806247441
use of [125i]deoxycytidine to detect herpes simplex virus-specific thymidine kinase in tissues of latently infected guinea pigs.the footpad skin and the lumbosacral dorsal root ganglia were removed from inbred guinea pigs at different times after subcutaneous infection with herpes simplex virus type 2 (hsv-2) in both hind footpads. these tissues, shown by our previous study to harbor latent hsv, were dispersed into single cells. the presence of virus-specific thymidine kinase (tk) in these cells was assayed by the uptake and phosphorylation of [125i]deoxycytidine in culture. [125i]deoxycytidine was shown to be a specific ...19806247502
genetic retrieval of viral genome sequences from herpes simplex virus transformed cells.oncogenic transformation of cultured cells by inactivated herpes simplex virus (hsv) types 1 and 2 has been demonstrated. expression of hsv information in these transformed cells has been shown by immunofluorescence studies, detection of hsv neutralizing antibody in sera from tumour-bearing animals and by hybridization of hsv-specific rna. molecular hybridization studies of dna from hsv-2 transformed hamster cells have detected up to 40% of the hsv genome present in several copies. complementati ...19806247659
[isolation of an herpes simplex virus type 2 from a human cervical cancer by means of a cell cocultivation]. 19806247704
[cellular immune response detected in blast transformation test with lymphocytes from rabbits immunized with nucleocapsid of herpes simplex type 2 virus].a preparation of herpes simplex virus type 2 (hsv-2) nucleocapsid (nc) was obtained after treatment of intracellular virus with detergents followed by centrifugation in linear sucrose density gradient. electron microscopic analysis revealed polymorphism of hsv-2 nc population. three main types of capsid structures were found: empty capsids, nc containing various amounts of nucleoid material, and nc with complete nucleoid. morphological heterogeneity correlated with heterogenous sedimentation pro ...19806247843
oxime and dithiolane derivatives of 5-formyl-2'-deoxyuridine and their 5'-phosphates: antiviral effects and thymidylate synthetase inhibition.5-formyl-2'-deoxyuridine (2a), an effective inhibitor of herpes simplex virus type 1 or 2 (hsv-1, hsv-2) and vaccinia virus, was converted to the oxime (3a) and dithiolane (4a) derivatives. the oxime (3a) was equally as potent as the formyl compound against hsv-1, but one-fifth as active against hsv-2, 100 times less effective against vaccinia, and 25 times less toxic for the host cells. in addition, compound 3a was about 10 times less active than 2a in inhibiting thymidylate synthetase in vivo ...19806248643
immunoprecipitation of polypeptides from hamster embryo cells transformed by herpes simplex virus type 2. 19806249032
combination chemotherapy: interaction of 5-methoxymethyldeoxyuridine with adenine arabinoside, 5-ethyldeoxyuridine, 5-iododeoxyuridine, and phosphonoacetic acid against herpes simplex virus types 1 and 2.the antiviral activity of 5-methoxymethyl-2'-deoxyuridine (mmudr) was compared with that of 5-iodo-2'-deoxyuridine (iudr), 5-ethyl-2'-deoxyuridine (etudr), adenine arabinoside (ara-a), and phosphonoacetic acid (paa) against herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2). mmudr was more potent than ara-a and paa but less active than etudr and iudr against hsv-1 in rabbit kidney (rk-13) cells. in vero cells, the antiviral activities of mmudr, ara-a, and paa against hsv-1 were of the same o ...19806249191
differences in attachment between herpes simplex type 1 and type 2 viruses to neurons and glial cells.fractions of nerve cell perikarya, synaptosomes, and astrocytic glia were prepared from human, monkey , rabbit, rat, and mouse brain tissue. the herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) binding capacity of these fractions was studied. pretreatment of fractions with one type of hsv and the subsequent testing of adsorption of homotypic and heterotypic virus ws employed to reveal type selectivity of virus binding receptors. a higher density of hsv-1 than of hsv-2 selective receptors w ...19806249749
cervical carcinoma: detection of herpes simplex virus rna in cells undergoing neoplastic change.3h-hsv-2 dna has been hybridized in situ to frozen sectioned tissue from human cervical biopsies. rna complementary to the virus-specific probe was detected in cells undergoing pre-malignant changes, but not in the cells of the fully developed squamous-cell cancer.19806249760
ag-4 complement-fixing antibodies in cervical cancer and herpes-infected patients using local herpes simplex virus type 2.the incidence of anti-ag-4 complement-fixing antibodies in australian cervical carcinoma (cacx), herpes simplex virus (hsv) type 1 and 2, and control patients, was investigated using local hsv strains. the local hsv strains (both hsv-imi and hsv-2mi) were found, by neutralization experiments, to vary from the american prototype strains. all hsv-2-strains tested were able to induce ag-4 in 4-h infected hep-2 cells. anti-ag-4-complement-fixing antibodies were detected in 40% of dysplasia patients, ...19806249764
elution of herpes simplex virus-specific cytotoxic antibodies from squamous cell carcinoma of uterine cervix.there is evidence that carcinoma of the cervix uteri is associated with infection with herpes simplex virus type 2 (hsv-2), and although indirect evidence suggests an aetiological relationship, this remains controversial. one facet of the problem is that complement-dependent cytotoxic antibodies is hsv-2-infected cells have been undetectable or found only in low titre in sera from patients with progressive cervical cancer whereas higher titres have been found where cervical lesions are less adva ...19806250079
[effect of the physiological status of culture on herpes simplex virus type 2 infection of synchronized cells bnk-21].infecting of synchronized cells-bnk-21 by herpes simplex virus type 2 in the s period resulted in a secondary rise of the labeled cells index and shortened time of the cell cycle as a whole. while infecting of the cells during the s period some morphological transformation was observed. the infecting of the cells in the periods m, g1, g2 revealed no essential changes in cell dna synthesis and failed to result in any cell transformation.19806250284
properties of herpes simplex virus type 1 and type 2 dna polymerase.herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) dna polymerases were highly purified from infected hela bu cells by deae cellulose, phosphocellulose and dna cellulose column chromatography. dna exonuclease activity but not endonuclease activity was found associated with both types of dna polymerase. both dna polymerase activities could be activated by salt in a similar fashion with the optimal activity in the range of ionic strength between 0.22 and 0.29 alpha. at an ionic strength of 0.1 ...19806250618
experimental infection and the state of viral latency of adult tupaia with herpes simplex virus type 1 and 2 and infection of juvenile tupaia with temperature-sensitive mutants of hsv type 2.the susceptibility of adult tupaia belangeri to infection with herpes simplex virus (hvs) was investigated. adult animals were inoculated intraperitoneally with hsv type 1 or 2. with the exception of hsv-2, strain hg-52, 10(5)--10(6) pfu of all hsv strains caused lethal infection irrespective of the age of the animals. infections hsv was recovered from the spinal cord of those animals which had survived infection with a low dose of virus. the dna of the recovered viruses was compared to the dna ...19806251787
herpes simplex virus phosphoproteins. ii. characterization of the virion protein kinase and of the polypeptides phosphorylated in the virion.the protein kinase associated with purified herpes simplex virus 1 and 2 virions partitioned with the capsid-tegument structures and was not solubilized by non-ionic detergents and low, non-inhibitory concentrations of urea. the enzyme required mg2+ or mn2+ and utilized atp or gtp. the activity was enhanced by non-ionic detergents and by na+ even in the presence of high concentrations of of mg2+, but not by cyclic nucleotides. the enzyme associated with capsid-tegument structures phosphorylated ...19806252339
temporal regulation of herpes simplex virus type 2 transcription and characterization of virus immediate early mrna's.nuclear and cytoplasmic virus rnas, synthesized in cells infected with herpes simplex virus type 2 at early and late times post-infection, and in the continuous presence of the protein synthesis inhibitor cycloheximide (immediate early), have been analyzed by blot hybridization to virus dna fragments generated by bam hi and eco ri restriction endonucleases. polyadenylated immediate early mrnas were separated on denaturing gels containing ch3hgoh giving three virus-specific mrna bands of estimate ...19806253886
responses of mouse spleen morphology to the growth of subcutaneously injected virally transformed cells.the changes in the fractional volume of six structural components in the spleens of balb/c mice injected with herpes simplex virus type 2-transformed cells (h238 tumor cells) were quantitated during progressive tumor growth. spleen stereology was performed at three time intervals during the early stages of tumor development. the results revealed that the volume of the compact myeloid tissue and reaction center of lymphoid nodules increased about four- to five-fold from 10 to 33 days after h238 t ...19806254396
comparison of indirect hemagglutination and 51chromium release tests for detection of herpes simplex virus type 1 and 2 antibodies in patients with recurrent herpes infections.indirect hemagglutination and 51cr release tests (ihat and 51-crt respectively) were compared in patients with recurrent herpes simplex virus (hsv) infections from whom hsv-1 or hsv-2 was isolated. both tests were equally sensitive and specific in detecting hsv antibodies. however, ihat was more specific in detecting homologous hsv antibody response in patients with recurrent hsv-2 infections. past infections with hsv-1 in the patients with dual infections were detected by determining hsv-type s ...19806254476
reaction patterns of herpes simplex virus type 1 and type 2 proteins with sera of patients with uterine cervical carcinoma and matched controls.serum from 105 individuals with diagnosed uterine cervical cancer and 231 matched controls were examined for their ability to react with a large number of herpes simplex virus type 1 or type 2 (hsv-1, hsv-2) proteins. radiolabeled hsv-1 or hsv-2 proteins were mixed with test serum and immune complexes were isolated with staphylococcal protein a. viral proteins in the immune complexes were resolved by polyacrylamide gel electrophoresis and visualized by fluorography. when the frequency of precipi ...19806254649
pathogenicity of wild-type and temperature-sensitive mutants of herpes simplex virus type 2 in guinea pigs.the pathogenicity of herpes simplex virus type 2 strain 186, the wild-type (wt) strain, and four temperature-sensitive (ts) mutants was studied after genital inoculation of female guinea pigs. infection with the wt virus was generally severe, with extensive skin lesions in 89% and mortality in 37% of inoculated animals. guinea pigs inoculated with ts mutants manifest remarkably mild disease, with lesions occurring in only 16% of the guinea pits and a mortality rate of 7%. wt virus was recovered ...19806254877
immunity to herpes simplex virus type 2: cell-mediated immunity in latently infected guinea pigs.cell-mediated (cmi) and humoral immunity to herpes simplex virus type 2 (hsv-2) were evaluated in infected strain 13/n guinea pigs with (45%) and without a history of recurrent herpetic disease. virus was isolated by cocultivation from active herpetic lesions (9 of 10) as well as from the footpads (17 of 38), sacral ganglia (7 of 21), and sciatic nerves (1 of 21) of asymptomatic animals. viral isolates grew in cells of human origin and were neutralized by hyperimmune anti hsv-2 sera. humoral imm ...19806254886
presence of herpes simplex virus type 2 (hsv-2) antibodies and antigens in patients with cervical carcinoma in thailand. 19806255050
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