Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| interaction of polylysine with the cellular receptor for herpes simplex virus type 1. | we earlier reported that neomycin blocked reversibly the binding of herpes simplex virus type 1 (hsv-1) to the receptor of bhk cells, while the binding of hsv-2 to the receptor was unaffected. we could not determine whether the effect was on the virus particle, the receptor, or both. we have now tested several other cationic substances, and report that polylysine (and polyarginine) block the binding of hsv-1 to the receptor by interfering with the cellular receptor function; higher molecular wei ... | 1988 | 2838567 |
| effect of herpes simplex virus type 2 infection on mitochondrial gene expression. | a cdna clone derived from the mitochondrial cytochrome oxidase i gene has been used to show that the level of mitochondrially encoded rna species declines during herpes simplex virus type 2 infection in a manner similar to that for rna species derived from nuclear genes. in contrast to the situation for nuclear genes, however, no change in the transcription rate of the mitochondrial genome during infection was detected, indicating that post-transcriptional processes alone are responsible for the ... | 1988 | 2838578 |
| purification of the herpes simplex virus type 1 65-kilodalton dna-binding protein: properties of the protein and evidence of its association with the virus-encoded dna polymerase. | using a combination of conventional column chromatography and velocity sedimentation, we have purified the 65-kilodalton dna-binding protein (65kdbp) encoded by herpes simplex virus (hsv) greater than 625-fold. the hsv type 1 (hsv-1)-encoded dna polymerase (pol) cofractionated with 65kdbp through deae-sephacel, blue sepharose, and mono q columns and was only separated from 65kdbp by sedimentation through a glycerol gradient. immunoaffinity columns containing monoclonal antibody (mab) 6898 immuno ... | 1988 | 2839706 |
| changing patterns of genital herpes simplex virus infections. | herpes simplex virus type 2 is considered by many venereologists as the predominant serotype among those patients suffering from genital herpes. however, identification of genital isolates obtained from 16 through 20, and 31 through 40 year old individuals among our female patient population shows a predominance of hsv-1 infections. these data suggest that the "above the waist"-"below the waist" epidemiologic patterns commonly ascribed to reflect a prevalence of hsv-1 and hsv-2 infections respec ... | 1988 | 2839940 |
| methyl gallate, methyl-3,4,5-trihydroxybenzoate, is a potent and highly specific inhibitor of herpes simplex virus in vitro. i. purification and characterization of methyl gallate from sapium sebiferum. | a potent anti-herpetic compound was identified and purified to homogeneity from the leaves of sapium sebiferum by plaque reduction assay using herpes simplex virus type 2. the chemical structure of the purified compound was determined by mass spectroscopy and proton and carbon-13 nuclear magnetic resonance as methyl gallate, methyl-3,4,5-trihydroxybenzoate. this is the first demonstration that methyl gallate is a potent anti-herpetic compound in vitro, present in high concentration in the leaves ... | 1988 | 2840132 |
| methyl gallate, methyl-3,4,5-trihydoxybenzoate, is a potent and highly specific inhibitor of herpes simplex virus in vitro. ii. antiviral activity of methyl gallate and its derivatives. | methyl gallate (mg), methyl-3,4,5-trihydroxybenzoate, was highly active against herpes viruses as determined by plaque reduction assay. herpes simplex virus type 2, ms strain, was sensitive to mg at a mean 50% inhibitory concentration (ic50) of 0.224 micrograms/ml in monkey kidney cells. mg was specific for herpes viruses with the relative sensitivity hsv-2 greater than hsv-1 greater than cmv. two rna viruses tested were significantly less sensitive to mg. the structural components of mg which m ... | 1988 | 2840133 |
| detection of herpes simplex virus type 2 dna in uterine cervix lesions using cloned bgl ii n fragment of hsv-2 dna as a probe. | 1988 | 2840390 | |
| effect of macrophage activation on resistance of mouse peritoneal macrophages to infection with herpes simplex virus types 1 and 2. | to define the effect of heterogeneity of murine peritoneal macrophages (m phi) on intrinsic resistance to herpes simplex virus (hsv) infection, several m phi populations were characterized for their response to infection with hsv type 1 (hsv-1) and hsv-2. steady-state resident m phi (res m phi) were compared in parallel with m phi activated with corynebacterium parvum (now designated propionibacterium acnes) (cp m phi) and thioglycollate-elicited inflammatory m phi (tg m phi). res m phi were com ... | 1988 | 2841412 |
| specific induction of cellular gene transcription in herpes simplex virus type 2-transformed cells. | in spite of much work, the mechanism of oncogenic transformation by herpes simplex virus (hsv) is as yet unknown. it has been proposed that hsv type 2 (hsv-2) can transform cells by a 'hit and run' mechanism. in the past we have demonstrated that several polypeptides can be immunoprecipitated from hsv-2-transformed cells, but not from control cells or adenovirus-transformed cells, by rabbit hyperimmune sera to hsv-2. it is possible that the expression of these proteins might be the result of act ... | 1988 | 2841413 |
| a cellular protein related to heat-shock protein 90 accumulates during herpes simplex virus infection and is overexpressed in transformed cells. | a monoclonal antibody produced from mice immunized with hsv-infected cell dna binding proteins reacts with two cell-encoded polypeptides, p90 and p40, synthesized constitutively by many different cell types and expressed at high levels in transformed cells. in uninfected cells heat shock induces a cytoplasmic accumulation of p90 and in agreement with this we show that p90 is related to hsp90. during hsv-2 lytic productive infection, p90 accumulates to very high levels, such accumulation being de ... | 1988 | 2842174 |
| in vivo behavior of genetically engineered herpes simplex viruses r7017 and r7020: construction and evaluation in rodents. | the herpes simplex virus (hsv) recombinant r7017 was constructed from hsv-1 (strain f) by deleting a portion of the thymidine kinase (tk) gene and by replacing the sequences representing the internal inverted repeats and adjacent genes in the l component with a fragment of the hsv-2 genome encoding the glycoproteins g, d, i, and a portion of e. in addition, the r7020 recombinant contains an hsv-1 dna fragment encoding the tk gene fused to the alpha 4 gene promoter. the results of studies in mice ... | 1988 | 2842408 |
| history of recurrent mucocutaneous herpes correlates with relatively low interferon production by herpes simplex virus-exposed cultured monocytes. | monocytes separated from blood specimens of healthy voluntary blood donors were infected with herpes simplex virus (hsv) 1 or 2. the amount of interferon produced by the hsv-exposed monocytes was found to be donor-specific and was similar whether tested with hsv 1 or hsv 2. the interferon levels in repeated blood specimens from a given donor were relatively stable within an observation period of a few months. the survey was expanded by testing 27 persons with history of recurrent herpes disease ... | 1988 | 2842448 |
| phosphonoformate and phosphonoacetate derivatives of 5-substituted 2'-deoxyuridines: synthesis and antiviral activity. | the synthesis of potential "combined prodrugs" wherein phosphonoformate or phosphonoacetate was attached to the 5'-position of 2'-deoxyuridine, 2'-deoxythymidine, 5-iodo-2'-deoxyuridine (idu), 5-(2-chloroethyl)-2'-deoxyuridine (cedu), or 5-(2-bromovinyl)-2'-deoxyuridine (bvdu) or to the 3'-position of cedu is described. the antiviral activities of these derivatives and of reference compounds were compared in vero, hep-2, and primary rabbit kidney cells against herpes simplex virus types 1 and 2 ... | 1988 | 2842506 |
| class-specific herpes simplex virus antibodies in sera and cervical secretions from patients with cervical neoplasia: a multi-group comparison. | serum and cervical secretions were collected from patients with cervical dysplasia, carcinoma-in-situ (cis), squamous cell carcinoma (cervical scc), and controls with normal cervices, attending clinics within the west lambeth health district, london. enzyme-linked immunosorbent assays were used to examine cervical secretory iga (siga) and serum igg and iga antibodies to herpes simplex virus (hsv). sexual and demographic factors were considered during data analysis, which involved fitting multipl ... | 1988 | 2837405 |
| a test for the relative potency of herpes simplex virus vaccines based upon the female guinea-pig model of hsv 2 genital infection. | an elisa for total herpes simplex virus (hsv) 1 antigen content and a test of immunogenicity based upon the female guinea-pig model of hsv 2 genital infection were applied to two samples from batches of hsv 1 subunit ('skinner') vaccine. the elisa was reproducible within an approximately threefold limit of error and batches 1 and 2 were indistinguishable in antigen content. the effects of vaccination in the guinea-pig model were assessed by a statistical analysis of scores derived from the princ ... | 1988 | 2836425 |
| transmission of genital herpes in couples with one symptomatic and one asymptomatic partner: a prospective study. | to determine the risk of transmission of genital herpes simplex virus (hsv) infection, we prospectively studied, for a median of six months, 38 couples who had been together for a median of 10 mo. in each couple, one partner had a history of symptomatic genital herpes and one did not. at entry, of the 38 asymptomatic, exposed partners, 21 were seronegative, and results of western blot analysis showed that seven had antibody to hsv type 1 (hsv-1), four to hsv type 2 (hsv-2), and six to both hsv-1 ... | 1988 | 2836518 |
| antibody to herpes simplex virus (hsv) icp-35 proteins after hsv challenge of animals immunized with hsv subunit vaccines. | the serological response to the icp-35 group of herpes simplex virus type 2 (hsv-2) proteins was examined 30 d after vaginal challenge with hsv-2 of guinea pigs previously immunized with hsv glycoproteins. detection of antibodies to icp-35 proteins correlated best with the magnitude of vaginal viral replication. animals that developed antibody to icp-35 proteins had more-frequent recurrences (p less than .0002) that were of longer duration (p less than .005) than animals that did not. by covaria ... | 1988 | 2836519 |
| antigen-specific immune-suppressor factor in herpes simplex virus type 2 infections of uv b-irradiated mice. | uv b-irradiation (280 to 320 nm) of mice at the site of cutaneous infection with herpes simplex virus type 2 (hsv-2) induced suppressor t-cell circuits that decreased hsv-2-induced proliferative responses of hsv-2-immune lymph node cells. adoptive transfer experiments indicated that splenocytes from uv b-irradiated hsv-2-infected animals contain l3t4+ cells that suppress proliferative responses in vivo, consistent with suppressor inducer cells. however, following in vitro culture of the splenocy ... | 1988 | 2836632 |
| monoclonal antibodies directed against a 130k glycoprotein of bovine herpesvirus 2 cross-react with glycoprotein b of herpes simplex virus. | envelope proteins of bovine herpesvirus type 2 (bhv-2) and herpes simplex virus types 1 and 2 (hsv-1, -2) share cross-reacting determinants. monoclonal antibodies directed against epitopes of a 130k glycoprotein of bhv-2 detect determinants on the surface of infected cells and react either with cytoplasmic or nuclear antigens. biological and biochemical characterization of the proteins recognized by these antibodies revealed that the bhv-2 specific 130k glycoprotein and the gb of hsv share commo ... | 1988 | 2837013 |
| incorporation into nucleic acids of the antiherpes guanosine analog buciclovir, and effects on dna and protein synthesis. | using cells expressing herpes simplex virus (hsv) thymidine kinase, we investigated the metabolism of the acyclic antiherpes guanosine analog buciclovir, in relation to the effects of the drug on viral dna and protein synthesis. in these cells the predominant metabolite of buciclovir was its triphosphate, as in the hsv-1 infected vero cells investigated in parallel. further metabolism of buciclovir led to incorporation into rna and dna. buciclovir inhibited dna synthesis, not rna synthesis, and ... | 1988 | 2837218 |
| comparison of western blot (immunoblot) and glycoprotein g-specific immunodot enzyme assay for detecting antibodies to herpes simplex virus types 1 and 2 in human sera. | sera from patients with culture-proven genital herpes infections were tested for herpes simplex virus type 1 (hsv-1)- and hsv-2-specific antibodies by both a western blot (immunoblot) technique (wba) and immunodot enzyme assays (ieas) specific for hsv-1 or hsv-2 glycoprotein g (gg). of 137 serum samples tested, none was mistyped by either wba or iea. both tests were most sensitive with sera obtained at least 21 days after onset of primary hsv-2 infections or sera drawn during recurrent hsv-2 gen ... | 1988 | 2835389 |
| efficacy of recombinant glycoprotein d subunit vaccines on the development of primary, recurrent, and latent genital infections with herpes simplex virus type 2 in guinea pigs. | vaccination of guinea pigs with recombinant glycoprotein d (subunit vaccine) from either herpes simplex virus type 1 or type 2 (hsv-1, hsv-2), before intravaginal inoculation with hsv-2, markedly altered viral replication in the vaginal tract and reduced, or completely prevented, the development and spread of external genital lesions. vaccination with glycoprotein d from hsv-2 prevented the establishment of latent hsv-2 infections in dorsal root ganglia and reduced the frequency of recurrent epi ... | 1988 | 2834467 |
| suppression of humoral immune response against herpes simplex virus induced by defective strains, ts- and tk- mutants. | suppression of humoral antibody formation against hsv is not only induced by replicating herpes simplex virus type 2 (hsv-2) but also by the defective strain ang and the deletion mutant 1301 of herpes simplex virus type 1 (hsv-1). moreover, ts-mutants a, h, k, s, 1201 and 1208 of hsv-1 as well as some ts-mutants of hsv-2 and "defective-interfering" particles of hsv-1 after high multiplicity of infection-passages induced suppression. treatment of infected mice with acg reduced antibody-formation ... | 1988 | 2835944 |
| on the control of immediate early (alpha) mrna survival in cells infected with herpes simplex virus. | the alpha or immediate early mrna of herpes simplex virus strain hsv-2(g) had a half-life of about 15 min if made in the absence of viral protein synthesis but was relatively stable if viral protein synthesis occurred, either freely or restricted by the presence of the proline analogue azetidine. in contrast, the alpha mrna of other strains of the virus is stable, even in the absence of protein synthesis. studies with recombinant viruses showed that the region of the viral dna between 0.58 and 0 ... | 1988 | 2846763 |
| herpes simplex virus (hsv)-specific proliferative and cytotoxic t-cell responses in humans immunized with an hsv type 2 glycoprotein subunit vaccine. | studies were undertaken to determine whether immunization of humans with a herpes simplex virus type 2 (hsv-2) glycoprotein-subunit vaccine would result in the priming of both hsv-specific proliferating cells and cytotoxic t cells. peripheral blood lymphocytes (pbl) from all eight vaccines studied responded by proliferating after stimulation with hsv-2, hsv-1, and glycoprotein gb-1. the pbl of five of these eight vaccines proliferated following stimulation with gd-2, whereas stimulation with gd- ... | 1988 | 2846864 |
| herpes simplex viruses lacking glycoprotein d are unable to inhibit virus penetration: quantitative evidence for virus-specific cell surface receptors. | herpes simplex virus (hsv) glycoprotein d (gd) plays an essential role in the entry of virus into cells. hsv mutants unable to express gd were constructed. the mutants can be propagated on vd60 cells, which supply the viruses with gd; however, virus particles lacking gd were produced in mutant-infected vero cells. virus particles with or without gd adsorbed to a large number (greater than 4 x 10(4] of sites on the cell surface; however, virions lacking gd did not enter cells. cells pretreated wi ... | 1988 | 2846873 |
| properties of two 5'-coterminal rnas transcribed part way and across the s component origin of dna synthesis of the herpes simplex virus 1 genome. | in cells infected with herpes simplex virus 1 the domains of the two identical origins of viral dna synthesis in the s component (oris) are transcribed in two 5'-coterminal rnas. orisrna1, synthesized early in infection and also in the absence of protein synthesis by infected cells, runs antisense to and terminates at the transcription initiation site for alpha genes 22 or 47. orisrna2, reported earlier, was detected in cells 14 hr after infection; it extends across the origin and is coterminal ... | 1988 | 2847162 |
| the cleavage recognition signal is contained within sequences surrounding an a-a junction in herpes simplex virus dna. | herpesvirus genome maturation involves site-specific cleavage of viral dna concatemers and encapsidation of unit-length molecules, processes that are apparently coupled. here, applying a transfection-infection approach, we have investigated the arrangement of the dna sequence elements involved in cleavage and shown that specific cleavage occurs independently of dna replication. we show that the cis-acting signal for cleavage is located within a 179-bp fragment from across an a-a junction formed ... | 1988 | 2847414 |
| infection of human thymic epithelial cells by human cytomegalovirus and other viruses: effect on secretion of interleukin 1-like activity. | cultures of human thymic epithelial (te) cells are able to produce a interleukin 1 (il-1) like activity. this il-1 activity can be detected either using mouse thymocytes in a traditional il-1 assay, or using thymic lymphocytes obtained from cases of pediatric cardio-vascular surgery. production of il-1 activity by te cells was found to be maximal between 3 and 4 weeks after culture initiation. human thymocytes worked best as targets in an il-1 assay, when these cells were derived from donors you ... | 1988 | 2844452 |
| effects of various nucleosides on antiviral activity and metabolism of 1-beta-d-arabinofuranosyl-e-5-(2-bromovinyl)uracil against herpes simplex virus types 1 and 2. | uptake of 1-beta-d-arabinofuranosyl-e-5-(2-bromovinyl)uracil (bv-arau) into herpes simplex virus type 1 (hsv-1)- and 2 (hsv-2)-infected cells was elevated about 190 to 40 times, compared with that into mock-infected human embryo lung fibroblast cells. uptake was not enhanced by infection with thymidine kinase-negative hsv-1 and hsv-2 mutants, however. in hsv-1-infected cells, 9.7% of bv-arau was phosphorylated to bv-arau triphosphate, but only 1.1% was phosphorylated in hsv-2-infected cells. the ... | 1988 | 2847634 |
| selective inhibition of human cytomegalovirus replication by a novel nucleoside, oxetanocin g. | a novel nucleoside with an oxetanosyl-n-glycoside has been recently isolated from a culture filtrate from bacillus megaterium and named oxetanocin a (n. shimada, s. hasegawa, t. harada, t. tomisawa, a. fujii, and t. takita, j. antibiot. 39:1623-1625, 1986). in this study, we evaluated the antiherpesvirus activity of oxetanocin a and its derivatives and found that 9-(2-deoxy-2-hydroxymethyl-beta-d-erythro-oxetanosyl)guanine (oxt-g) was very potent and selective in inhibiting the replication of hu ... | 1988 | 2847638 |
| correlation of histologic types of carcinoma of the uterine cervix and human papillomavirus and herpes simplex virus type 2 dna sequences in the uterine cervical biopsies. | forty patients with invasive cancer of cervix from new delhi were examined for the presence of human papillomavirus (hpv) and herpes simplex virus (hsv) type 2 dna sequences in a southern hybridization assay. data were analysed according to the histologic type of cancer. hpv dna sequences were detected in 82.5% biopsies and hsv-2 dna sequences were found in 10% biopsies. hpv-16 dna sequences were found either alone or together with hpv-18 and/or hsv-2 bgl ii n fragment in 55% biopsies from kerat ... | 1988 | 2848476 |
| the effect of triterpenoid compounds on uninfected and herpes simplex virus-infected cells in culture. iii. ultrastructural study of virion maturation. | electron microscope studies been made of mock-infected or herpes simplex virus (hsv) type 1 (strain 17)- or hsv-2(strain hg52)-infected flow 2002 cells grown in the absence or presence of various concentrations of cicloxolone sodium (ccx). fifty non-serial, thin sections of mock-infected or hsv-infected cells, which contained a portion of the nucleus, were examined by transmission electron microscopy. with increasing drug concentration, counts of capsid structures both in the nucleus and cytopla ... | 1988 | 2828518 |
| ability of herpes simplex virus (hsv) types 1 and 2 to induce clinical disease and establish latency following previous genital infection with the heterologous hsv type. | guinea pigs were infected intravaginally with either herpes simplex virus type 1 (hsv-1) or type 2 (hsv-2). one month postinoculation, animals were inoculated with the heterologous hsv type and observed for clinical disease and shedding of virus. one month after superinfection, animals were killed, and tissues were cocultivated to detect latent virus. although the severity of clinical disease and the degree of shedding of virus were greatly reduced by prior infection with the heterologous virus ... | 1988 | 2848899 |
| chronic-dose acyclovir to suppress frequently recurring genital herpes simplex virus infection: effect on antibody response to herpes simplex virus type 2 proteins. | to determine the effect of prolonged suppressive acyclovir therapy on the antibody response to herpes simplex virus type 2 (hsv-2) proteins, we studied sequential sera from 33 patients with frequently recurring (six or more recurrences per year) genital herpes. twenty-two patients received 400 mg of oral acyclovir and 11 received placebo, twice daily for one year. sera collected at enrollment, after six months and 12 months of therapy, and during the first recurrence after cessation of therapy w ... | 1988 | 2848900 |
| herpes simplex virus type 2 latency in the footpad of mice: effect of acycloguanosine on the recovery of virus. | herpes simplex virus type 2 has been reactivated from the latent state in the footpad and dorsal root ganglia of acycloguanosine-treated balb/c mice. virus was also recovered from the footpad tissue but not from the ganglia of denervated, latently infected mice. treatment in vitro of explanted footpad cultures with acycloguanosine or phosphonoacetic acid did not affect the rate of virus reactivation. in all the isolates examined the virus was found to be acycloguanosine-sensitive. recovery of vi ... | 1988 | 2828517 |
| herpes simplex virus type 2 establishes latency in the mouse footpad. | balb/c mice were infected with herpes simplex virus type 2 by inoculation into the footpad. three months or more after infection, and in the total absence of any evidence of illness, latent virus could be reactivated from both ganglia and footpad tissue but only after explantation and culturing for a week or more. virus recovery from the footpad could not be prevented by treatment of the infected mice with acycloguanosine for up to 6 months in their drinking water at 40 mg/kg, nor by femoral and ... | 1988 | 2828516 |
| herpes simplex virus ii antigen in adenocarcinoma of the uterine cervix. | 1988 | 2844663 | |
| mutational analysis of the herpes simplex virus type 1 trans-inducing factor vmw65. | the herpes simplex virus type 1 (hsv-1) polypeptide vmw65 is a structural component of the virus particle and is also responsible for trans-induction of immediate early (ie) transcription. functional domains of this polypeptide were investigated by constructing a series of 10 plasmids each with a 12 bp insertion in the gene encoding vmw65. plasmids were analysed for their ability to stimulate ie transcription in short term transfection assays, and the altered vmw65 polypeptides were assayed for ... | 1988 | 2844968 |
| detection of herpes simplex virus type 1 in herpetic ocular diseases by dna-dna hybridization using a biotinylated dna probe. | a diagnostic hybridization assay for detecting herpes simplex virus type 1 (hsv-1) in ocular specimens was developed using cloned viral dna as a probe. this hybridization assay is based on visualizing a biotinylated probe that is hybridized to the target dna by a streptavidin/alkaline phosphatase system. the time required for performing this assay system is only two days. this assay system could detect a probe which had been hybridized to as little as 1 pg of homologous dna and did not cross-rea ... | 1988 | 2844977 |
| c3b receptor activity on transfected cells expressing glycoprotein c of herpes simplex virus types 1 and 2. | glycoprotein c from herpes simplex virus type 1 (gc-1 from hsv-1) acts as a receptor for the c3b fragment of the third component of complement on hsv-1-infected cell surfaces. direct binding assays with purified gc-1 and c3b demonstrate that other viral and cellular proteins are not required for this interaction. although c3b receptor activity is not expressed on hsv-2-infected cell surfaces, purified gc-2 specifically binds c3b in direct binding assays, suggesting that gc-1 and gc-2 are functio ... | 1988 | 2845122 |
| anti hsv-2 properties of amniotic fluid. | some antiviral properties of amniotic fluid (af) were studied. a close relationship between maternal blood and af antiviral antibodies was seen. substances other than antibodies which could have an effect on viruses were assumed to be present in af. using hsv2 as a model, we found a virus-neutralizing activity in 6 out of 7 specimens studied which does not appear to be related to antibodies. | 1988 | 2845231 |
| modification of the genital herpes infection in a guinea pig model, by prior immunization with bovine herpes mammillitis virus. | bovine herpes mammillitis virus has been shown to partially protect guinea pigs against primary genital herpes simplex virus type 2 infections, further confirming the immunologic cross-reactivity of these two viruses. | 1988 | 2845232 |
| inhibition of terminal n- and o-glycosylation specific for herpesvirus-infected cells: mechanism of an inhibitor of sugar nucleotide transport across golgi membranes. | the nucleoside analog (e)-5-(2-bromovinyl)-2'-deoxyuridine (bvdu) inhibited the golgi-associated terminal glycosylation in herpes simplex virus type 1- and type 2-infected cells, specifically incorporation of galactose and sialic acid into n-linked oligosaccharides, and incorporation of sialic acid and, to a lesser extent, of galactose into o-linked oligo saccharides. this resulted in formation of viral glycoproteins with terminal glcnac and fuc in n-linked oligosaccharides and terminal o-linked ... | 1988 | 2845656 |
| an acyclovir-resistant strain of herpes simplex virus type 2 which is highly virulent for mice. | herpes simplex virus type 2 (hsv-2), strain ys-4 c-1, isolated by plaque cloning from a clinical isolate was found to be resistant to acyclovir (acv; acycloguanosine) in vitro. it was sensitive to phosphonoacetic acid and 9-beta-d-arabinofuranosyladenine. thymidine kinase (tk) activity of ys-4 c-1 was less than 1% of that of other strains from the same clinical source. however, thymidine plaque autoradiography showed that ys-4 c-1 was not completely deficient in tk activity. ys-4 c-1 showed high ... | 1988 | 2845890 |
| interaction of herpes simplex virus type 2 with a rat glioma cell line. | the interaction between herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) and two neural cell lines, mouse neuroblastoma (n1e-115) and rat glioma (c6-bu-1), was investigated. n1e-115 cells were permissive to both types of hsv. in c6-bu-1 cells, on the other hand, all the hsv-1 strains tested so far showed persistent infection, and the infectious virus of hsv-2 strains disappeared spontaneously. the hsv-2-infected c6-bu-1 cells were positive for hsv-2-specific dna sequences, virus-specific r ... | 1988 | 2850449 |
| herpes simplex virus induces the replication of foreign dna. | plasmids containing the simian virus 40 (sv40) dna replication origin and the large t gene are replicated efficiently in vero monkey cells but not in rabbit skin cells. efficient replication of the plasmids was observed in rabbit skin cells infected with herpes simplex virus type 1 (hsv-1) and hsv-2. the hsv-induced replication required the large t antigen and the sv40 replication origin. however, it produced concatemeric molecules resembling replicative intermediates of hsv dna and was sensitiv ... | 1988 | 2850486 |
| neurovirulence of individual plaque stocks of herpes simplex virus type 2 strain hg 52. | the virulence of ten independent plaque stocks of hsv-2 strain hg 52 was determined by intracranial inoculation of 3 week old balb/c mice. the ten stocks exhibited a range of ld50 which could be grouped into three classes; high (less than 10(3) pfu/mouse), intermediate (10(3)-10(4) pfu/mouse) and low (greater than 10(5) pfu/mouse). analysis of the ten stocks using six different restriction endonucleases showed no significant variation in the size and distribution of fragments. isolates from the ... | 1988 | 2850778 |
| effect of progesterone on the replication of herpes simplex virus type 2 in vitro. | this project involved an investigation into the effect of progesterone on the replication of herpes simplex virus type 2 in a vero-cell culture. the first part of the experiment relates to preincubation of the cells with progesterone in the duration of 48 hours. after innoculation, replication of the virus was continued in the presence of progesterone. samples for titration were taken 10, 21 and 32 hours after innoculation for the purpose of determining the single-cycle replication curve of herp ... | 1988 | 2834117 |
| effect of progesterone and pregnancy on the replication of herpes simplex virus type 2 in vivo. | this project involved an investigation into the effect of pregnancy and progesterone on genital herpes simplex virus (hsv) type 2 infection in mice, with the aim of establishing whether elevated progesterone level, due either to pregnancy or parenteral administration, makes the vaginal hsv infection all the heavier. the investigation was carried out on three groups of mice, representing three test situations in connection with vaginal hsv infection: the first group consisted of non-pregnant expe ... | 1988 | 2834118 |
| the use of colloidal gold immunoelectron microscopy to diagnose varicella-zoster virus (vzv) infections by rapid discrimination between vzv, hsv-1 and hsv-2. | colloidal gold immunoelectron microscopy was used to diagnose rapidly 53 cases clinically suspected of varicella-zoster virus (vzv) infection and one special case selected from another study on typical herpes simplex virus (hsv) infections. the viruses were identified and subsequently typed within 2.5 h by a direct labelling test for vzv, and within 3.5 h by an indirect labelling test with monoclonal antibodies against hsv type 1 and type 2. the protein a purified igg fraction of human anti-vzv ... | 1988 | 2851604 |
| secretory iga against herpes simplex virus in cervical secretions. | a recently developed method to recover proteins from cervical secretions was combined with methods to detect minute concentrations of herpes simplex virus (hsv) type specific antibodies to measure the concentrations of locally produced antibodies in women with genital infections. forty nine women attending a sexually transmitted disease (std) clinic were included. cervical secretions were obtained by suction into a plastic catheter. soluble proteins were recovered from the secretions by elution ... | 1988 | 2852157 |
| does neoplasia in-situ develop due to the interaction of epstein-barr virus or herpes simplex virus-2 with langerhans cells in the epithelium? | 1988 | 2852413 | |
| broad-spectrum antiviral activity of the acyclic guanosine phosphonate (r,s)-hpmpg. | (r,s)-9-(3-hydroxy-2-phosphonomethoxypropyl)guanine [(r,s)-hpmpg] exhibits broad spectrum antiviral activity with an ed50 of less than 1 microm against herpes simplex virus (hsv) types 1 and 2, varicella zoster virus, human cytomegalovirus (hcmv) and vaccinia in plaque reduction assays. wild type hsv-2 and its thymidine kinase deficient variant are equally sensitive to (r,s)-hpmpg. (r,s)-hpmpg is 100-fold more potent than acyclovir (ed50 = 0.45 microm vs. 44 microm, respectively) against hcmv in ... | 1988 | 2852486 |
| inhibition of herpes simplex virus type 2 replication in vitro by 1-n-pentadecanoyl-3''-n-trifluoroacetyl kanamycin a. | the in vitro antiviral activity as well as the mechanism of action of a new antiviral agent, a kanamycin analogue, 1-n-pentadecanoyl-3''-n-trifluoroacetyl kanamycin a (ptka) against herpes simplex virus type 2 (hsv-2) was investigated. the drug showed excellent antiviral action with negligible cytotoxic effect on the culture cells. based on plaque reduction assays the 50% inhibitory dose (id50) of the drug was 1 microgram/ml, and at 20 micrograms/ml plaque formation was totally suppressed. the c ... | 1988 | 2839107 |
| herpes simplex virus glycoprotein treatment of recurrent genital herpes reduces cervicovaginal virus shedding in guinea pigs. | treatment of previously infected guinea pigs with herpes simplex virus (hsv) glycoproteins reduces the frequency and severity of subsequent genital recurrences. an effective vaccine should also reduce episodes of viral shedding. in this study, hsv glycoproteins b and d treatment of animals experiencing recurrent genital herpes reduced the frequency of both clinical recurrences and cervicovaginal viral shedding. when virus was shed, however, the peak viral titer and duration of shedding was unalt ... | 1988 | 2852922 |
| homology of the hsv-2 "a-sequence" to cellular sequences. | bgl-ii fragments of the genome of herpes simplex virus type 2 (hsv-2) hg-52 were cloned into the vector p-neo and were used to screen the complete hsv-2 genome for regions cross-hybridizing with the genome of hel cells. most extensive cross-hybridizing activity was observed with a 530 bp sstii subfragment of the viral bamhi g dna-fragment (contained in bgl ii f), which spans the joint and the viral a-sequence. from a lambda-l47 library, a cellular 15 kb hindiii dna fragment was subcloned in pbr ... | 1988 | 2853486 |
| human papillomavirus and herpes simplex virus in vulvar squamous cell carcinoma in situ. | human papillomavirus deoxyribonucleic acid were detected in tissue specimens from 38 to 46 patients (83%) with squamous cell carcinoma in situ of the vulva. herpes simplex virus type 2--related antigen icsp 34/35 was demonstrated in 23 of the lesions (50%), and antibodies to herpes simplex virus types 1 and 2 were found in 74% and 65% of the serum samples tested, respectively. both human papillomavirus deoxyribonucleic acid and herpes simplex virus type 2 antigen were detected in 19 cases (41%). ... | 1988 | 2834955 |
| dna sequence and rna transcription through a site of recombination in a non-neurovirulent herpes simplex virus intertypic recombinant. | re6 is a herpes simplex type-1 (hsv-1) x herpes simplex type-2 (hsv-2) intertypic recombinant that cannot replicate in the adult mouse nervous system. in the accompanying report, we have shown that hsv-1 sequences between 0.698 and 0.721 map units can restore a partial neurovirulent phenotype to re6. in this report, we have used comparative dna sequence analysis of re6, 17syn+ (hsv-1) and hg52 (hsv-2) to demonstrate that this region contains a site of recombination between hsv-1 and hsv-2 sequen ... | 1988 | 2854325 |
| influence of sera from patients affected by neoplasia on some human macrophage functions. | it has been repeatedly reported that several functions of mononuclear cells are impaired in patients affected by neoplasia. moreover, inhibitory activity of serum and tumor extracts on macrophages have been described. in a previous study, we found a marked impairment of the intrinsic antiviral activity of macrophages derived from monocytes isolated from peripheral blood of patients with breast carcinoma or melanoma compared with that from blood of normal subjects. the aim of the present work was ... | 1988 | 2846174 |
| vaccine induced hsv 1 antibodies fail to correlate with protection against hsv 2 in guinea pigs. | guinea pigs immunised with hsv 1 subunit vaccine were bled, and subsequently challenged intravaginally with hsv 2. the clinical response to virus challenge was quantified, and correlations were sought between clinical score and virus-specific serum antibody titre in functional and binding assays (elisa, neutralization, complement-mediated cytotoxicity and antibody-dependent cellular cytotoxicity). no significant relationships were found, and it was concluded that reactivity in the serological as ... | 1988 | 2856207 |
| [herpes simplex virus type 2 antibody levels in sera from various age groups using elisa and ifat]. | we have investigated hsv-2, igg antibody levels in 180 sera which were obtained from 60 girl students between 18-20 years old, 60 pregnant women between 19-39 years old and 60 young men between 20-22 years old, by using elisa (enzyme linked immunosorbent assay) and ifat (indirect fluorescent antibody technique). all of the sera obtained from girl students and pregnant women were found negative for hsv-2, igg antibody by elisa. however 2 of 60 young men's sera were positive. totally 2 of 180 sera ... | 1988 | 2856217 |
| antiviral activities of 2'-deoxyribofuranosyl and arabinofuranosyl analogs of sangivamycin against retro- and dna viruses. | eight sugar-modified pyrrolopyrimidine nucleoside analogs related to the antibiotic sangivamycin were evaluated in cell culture against herpes simplex types 1 (hsv-1) and 2 (hsv-2), cytomegalovirus (cmv), adenovirus, and visna virus. five of the compounds were highly active against most of the viruses with 50% inhibition (ed50) values of 1-10 microm. the selectivity of the agents was low, with inhibition of uninfected cell proliferation occurring within 5-fold that of the virus ed50 for most of ... | 1988 | 2854957 |
| antiherpes activity of the immunomodulator ok-432, a streptococcal preparation, in immunosuppressed mice. | the antiviral activity of ok-432, an antitumor agent originating from streptococcal preparations, against herpes simplex virus type 2 (hsv-2) was investigated in mice immunosuppressed by cyclophosphamide (cy). intraperitoneal administration of ok-432 to mice 1 day after treatment with 200 mg cy/kg prevented death due to hsv-2 encephalitis in a dose-dependent manner. when the immunosuppressed mice were given ok-432 prior to hsv-2 infection, both by the intraperitoneal route, virus growth in the p ... | 1988 | 2854959 |
| regulation of glycoprotein d synthesis: does alpha 4, the major regulatory protein of herpes simplex virus 1, regulate late genes both positively and negatively? | earlier studies have described the alpha 4/c113 baby hamster kidney cell line which constitutively expresses the alpha 4 protein, the major regulatory protein of herpes simplex virus 1 (hsv-1). introduction of the hsv-1 glycoprotein b (gb) gene, regulated as a gamma 1 gene, into these cells yielded a cell line which constitutively expressed both the alpha 4 and gamma 1 gb genes. the expression of the gb gene was dependent on the presence of functional alpha 4 protein. in this article we report t ... | 1988 | 2824843 |
| entry of herpes simplex virus 1 in bj cells that constitutively express viral glycoprotein d is by endocytosis and results in degradation of the virus. | the bj cell line which constitutively expresses herpes simplex virus 1 glycoprotein d is resistant to infection with herpes simplex viruses. analysis of clonal lines indicated that resistance to superinfecting virus correlates with the expression of glycoprotein d. resistance was not due to a failure of attachment to cells, since the superinfecting virus absorbed to the bj cells. electron microscopic studies showed that the virions are juxtaposed to coated pits and are then taken up into endocyt ... | 1988 | 2824844 |
| a human cytomegalovirus function inhibits replication of herpes simplex virus. | human embryonic lung (hel) cells infected with human cytomegalovirus (hcmv) restricted the replication of herpes simplex virus type 1 (hsv-1). a delay in hsv replication of 15 h as well as a consistent, almost 3 log inhibition of hsv replication in hcmv-infected cell cultures harvested 24 to 72 h after superinfection were observed compared with controls infected with hsv alone. treatment of hcmv-infected hel cells with cycloheximide (100 micrograms/ml) for 3 or 24 h, conditions known to result i ... | 1988 | 2824846 |
| herpes simplex virus glycoprotein treatment of recurrent genital herpes. | we investigated the possibility that herpes simplex virus (hsv) glycoproteins might provide potential immunotherapy for recurrent hsv disease by using the guinea pig model of genital hsv infection. in two experiments, after recovery from initial genital herpes, 58 animals were randomized either to receive a glycoprotein vaccine or to serve as controls. both a mixture of hsv-2 glycoproteins derived from infected tissue cultures and a mixture of hsv-1 glycoproteins b and d, prepared by genetic eng ... | 1988 | 2826603 |
| generation of a herpes simplex virus type 2 variant devoid of xbai sites: removal of the 0.91 map coordinate site results in impaired synthesis of glycoprotein g-2. | herpes simplex virus (hsv) type 2 (strain hg52) has four xbai sites at map coordinates (m.c.) 0.45, 0.7, 0.91 and 0.94, i.e. two in the unique long and two in the unique short regions of the genome. previously, we had isolated a genome containing only the 0.45 and 0.94 xbai sites. here we report the isolation of a mutant (jh2611) in which all four xbai sites have been removed using an enrichment selection procedure, without any loss of viability. removal of each site has been shown to be due to ... | 1988 | 2826660 |
| herpes simplex virus infection of the hand. a profile of 79 cases. | seventy-nine cases of herpes simplex (hsv) infection of the hand were documented by viral culture over a 40-month period. a marked bimodal age distribution was found with 32 cases occurring in adults aged 21 to 30 years and 16 occurring in children less than 10 years old. the infection most commonly occurred on the fingers (69 percent) and thumb (21 percent). all 20 herpes simplex type 2 (hsv-2) isolates came from patients over 20 years old. all of the 13 specimens that were typed after isolatio ... | 1988 | 2827469 |
| enhanced in vitro reactivation of latent herpes simplex virus from neural and peripheral tissues with hexamethylenebisacetamide. | we evaluated the effect of the demethylating agent hexamethylenebisacetamide on reactivation of latent herpes simplex virus type 2 (hsv-2) from guinea pig neural and extraneural tissues. four explant cultures from the dorsal root ganglia of 42 latently infected guinea pigs and vaginal and cervical explant cultures from 33 animals were divided so that half received 5 mm of hexamethylenebisacetamide supplemented media and half media alone. hsv-2 was recovered earlier and from a greater percentage ... | 1988 | 2833203 |
| human papillomavirus, herpes simplex virus and cervical cancer incidence in greenland and denmark. a population-based cross-sectional study. | in greenland, the incidence of cervical cancer is 5.7 times higher than in denmark among women aged 20-39. from nuuk (greenland) and nykøbing falster (denmark) a sample of 800 women aged 20-39 years was drawn at random. a total of 586 and 661 women were investigated in greenland and denmark respectively. all women had a gynecological examination including a pap-smear and cervical scrape for hpv-analysis (filter in situ hybridization). a blood sample was taken for analysis of hsv type-specific an ... | 1988 | 2833450 |
| the epidemiology of infection with the human herpesviruses in navajo children. | investigations of serum antibody status to the five human herpesviruses--herpes simplex virus type 1, herpes simplex virus type 2, cytomegalovirus, epstein-barr virus, and varicella-zoster virus--were conducted on 197 navajo children, aged 1-15 years, at a reservation pediatric outpatient clinic in crownpoint, new mexico, from 1981-1984. to determine the severity of infection with the human herpesviruses, the authors reviewed lifetime medical records of 166 of the children for evidence of herpes ... | 1988 | 2833854 |
| antiviral activity of chamaecyparis lawsoniana extract: study with herpes simplex virus type 2. | ethanolic extract of the green part of chamaecyparis lawsoniana was tested for antiviral activity and toxicity in tissue culture. all experiments were carried out in confluent human embryo lung fibroblasts. treatment of fibroblast cells with extracts after viral inoculation was effective in inhibiting the replication of herpes simplex virus type 2 (hsv-2). the critical time for virus inhibition was 4 to 5 h after virus absorption. the antiviral activity was assayed employing the techniques of vi ... | 1988 | 2832710 |
| insertion and extension of acyclic, dideoxy, and ara nucleotides by herpesviridae, human alpha and human beta polymerases. a unique inhibition mechanism for 9-(1,3-dihydroxy-2-propoxymethyl)guanine triphosphate. | the ability of human alpha and beta dna polymerases and herpes simplex virus type 2 (hsv-2) and human cytomegalovirus (hcmv) dna polymerases to insert and extend several nucleotide analogs has been investigated using a variation of sanger-coulson dna sequencing technology. the analogs included the triphosphates of two antiviral nucleosides with incomplete sugar rings: 9-(1,3-dihydroxy-2-propoxymethyl)guanine (dhpg) and 9-(2-hydroxyethoxymethyl)guanine (acyg or acyclovir), as well as dideoxy and ... | 1988 | 2831212 |
| evidence that the gene for herpes simplex virus type 1 dna polymerase accounts for the capacity of an intertypic recombinant to spread from eye to central nervous system. | hsv-1(17) replicates 100-fold more efficiently than hsv-2(186) within trigeminal ganglia following ocular infection. in order to identify the nucleotide sequences responsible for the differences in the capacity of the two hsv strains to grow within the peripheral nervous system, an intertypic recombinant was generated by infecting neuroblastoma cells with hsv-2(186) and a hsv strain possessing nucleotide sequences from hsv-1(17). the genome of the intertypic recombinant was composed entirely of ... | 1988 | 2831653 |
| zwitterionic detergent solubilisation of hsv-1 surface antigens. | a preparation was obtained from herpes simplex virus type 1 (hsv-1)-infected cells using a zwitterionic detergent, empigen bb. the preparation was partially-purified either by ultracentrifugation over a cusion of 20% sucrose or on a sucrose density gradient. partial characterisation of these materials by elisa, using both polyclonal and monoclonal antibodies showed them to contain at least four major hsv glycoproteins, gb, gc, gd and ge. comparison of empigen-extracted hsv-1 antigen preparations ... | 1988 | 2831853 |
| genital virus infection and cervical neoplasia. | the reported association between genital infection by herpes simplex virus (hsv) type 2 and cervical neoplasia has been investigated at a molecular level. tissue culture of over 50 cervical cancers resulted in the development of several cell lines but none of these expressed hsv information. the permanent cell line, cx106, which was generated was found to contain multiple genome copies of human papillomavirus (hpv) type 16. southern blot analysis of nine cervical cancers showed an authentic 2.8 ... | 1988 | 2831934 |
| synthesis and biological activities of 4-o-(difluoromethyl)-5-substituted-uracil nucleoside analogues. | various 4-o-difluoromethyl analogues of 5-substituted uridine (urd), 2'-deoxyuridine (durd), and arabinofuranosyluracil (arau) nucleosides were prepared via a cf2-insertion reaction into 4-o-silylated nucleosides and evaluated for activity against herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) and cytotoxicity in human embryonic lung fibroblast (helf) cell cultures. the introduction of the 4-substituent led to a strong reduction of antiviral activity for durd but not for arau analogues. ... | 1988 | 2828623 |
| herpes simplex virus virion host shutoff function. | herpes simplex virus (hsv) virions contain one or more functions which mediate the shutoff of host protein synthesis and the degradation of host mrna. hsv type 1 (hsv-1) mutants deficient in the virion shutoff of host protein synthesis (vhs mutants) were isolated and were found to be defective in their ability to degrade host mrna. furthermore, it was found that viral mrnas in cells infected with the vhs 1 mutant have a significantly longer functional half-life than viral mrnas in wild-type viru ... | 1988 | 2828686 |
| prior herpes simplex virus type 2 infection as a risk factor for hiv infection. | antibodies to cytomegalovirus (cmv), epstein-barr virus (ebv), and herpes simplex viruses types 1 and 2 (hsv-1, hsv-2) in three sequential serum samples from 62 men who did and 61 men who did not develop human immunodeficiency virus (hiv) infection at the time of the final (third) serum specimen were studied. antibody titers to cmv, ebv, and hsv-1 did not significantly rise in or differ between men who did or did not get hiv infection. however, we found that 32 (68%) of 47 hiv seroconverters had ... | 1988 | 2828700 |
| flow cytometric detection of herpes simplex virus type 2 infected and transformed cells by immunoenzymatic and by indirect immunofluorescence staining. | the glucose oxidase antiglucose oxidase (gag) immunoenzymatic staining procedure has been used to detect herpes simplex virus (hsv) antigens microscopically. in this study, the gag procedure was adapted to cells in suspension, and its potential usefulness in flow cytometry was examined. hsv-2 infected monkey kidney and hsv-2 transformed mouse cells were stained using antisera to hsv-2 or to an hsv-2 specific protein with a molecular weight of 38 kd, respectively, with the gag procedure. flow cyt ... | 1988 | 2452054 |
| enhanced efficacy of nucleoside analogs and recombinant alpha interferon in weanling mice lethally infected with herpes simplex virus type 2. | weanling icr swiss mice were inoculated intranasally with a lethal dose of herpes simplex virus type 2, and treated with either vidarabine, vidarabine-5'-monophosphate, acyclovir or a hybrid recombinant human alpha interferon which is active in murine tissues. treatment with antiviral drugs was initiated 2, 24, 48, 72 or 96 h following virus inoculation. single drug treatment showed little effect on mortality, with only acyclovir showing some slight reduction. four dual drug combinations (vidara ... | 1988 | 2455473 |
| enhanced in vitro reactivation of herpes simplex virus type 2 from latently infected guinea-pig neural tissues by 5-azacytidine. | 5-azacytidine (5-azc) reduces cytosine methylation in dna and has been reported to activate quiescent virus genes. treatment of explant cultures of latently herpes simplex virus type 2 (hsv-2)-infected guinea-pig dorsal root ganglia and spinal cords in vitro with 5-azc significantly enhanced the rate of hsv recovery. both the number of isolates from ganglia (p less than 0.001) and the rate of recovery (p less than 0.001) were significantly increased with the addition of 50 microm-5-azc to explan ... | 1988 | 2453606 |
| anal cancer in women. | we studied predisposing factors in 56 women with anal cancer, comparing them with 56 matched controls drawn from the population. a detailed pretested questionnaire was administered to each study subject in a structured interview and blood was drawn for detection of herpes simplex virus antibodies by radioimmunoassay. pathologic material from cases was obtained and evidence of human papilloma virus infection was sought. by univariate analyses we found associations between anal cancer and positive ... | 1988 | 2836255 |
| prevalence of herpes simplex virus type 2 antibodies in selected population groups in italy. | the estimated prevalence of herpes simplex virus type-2 specific antibodies was less then 0.1% in a national sample of 1,169 healthy young males, 4.8% among 411 health care workers and 55% among 397 homosexuals. these viral infections seem to pose a health problem for risk groups such as homosexuals but not for the general population. | 1988 | 2837392 |
| increased efficacy of phosphonoformate and phosphonoacetate inhibition of herpes simplex virus type 2 replication by encapsulation in liposomes. | phosphonoformate and phosphonoacetate encapsulated in liposomes have substantially greater activity against herpes simplex virus type 2 in vero cell tissue culture than the nonencapsulated compounds at the same dose. encapsulation of phosphonoformate in liposomes resulted in a 30-fold increase of the antiviral effect with no increase in cytotoxicity measured by inhibition of thymidine incorporation into normal vero cells. thus, the selectivity of the liposomal drug increased 27-fold compared wit ... | 1988 | 2843083 |
| hypomethylation of host cell dna synthesized after infection or transformation of cells by herpes simplex virus. | infection of rat embryo cells with herpes simplex virus type 2 caused undermethylation of host cell dna synthesized during infection. dna made prior to infection was not demethylated, but some of its degradation products, including methyl dcmp, were incorporated into viral dna. the use of mutant virus showed that some viral dna synthesis appears to be required for the inhibition of methylation. inhibition of methylation cannot be explained by an absence of dna methyltransferase as the activity o ... | 1988 | 2837642 |
| use of routine viral cultures at delivery to identify neonates exposed to herpes simplex virus. | we obtained specimens for viral culture from mothers, infants, or both at the time of 6904 deliveries, without regard to the mothers' history of genital herpes. herpes simplex virus (hsv) was recovered in cultured specimens from 14 of the 6904 deliveries (0.20 percent); all 14 mothers were asymptomatic. all viral isolates were herpes simplex virus type 2 (hsv-2). only 1 of the 14 women (7 percent) had a history of genital herpes, whereas 12 (86 percent) had serologic evidence of a previous infec ... | 1988 | 2832756 |
| regulation of viral and cellular genes in a human neuroblastoma cell line latently infected with herpes simplex virus type 2. | a latent state of the herpes simplex virus type 2 genome was established in a human neuroblastoma cell line (sms-kcnr) to initiate studies on the mechanism by which host cells interact and regulate latent viral genes. to establish viral latency, it was necessary to prevent virus replication by briefly exposing the infected cells to antiherpetic acycloguanosine (20 microm) and human interferon (120 u/ml). subsequently however, these cells could be propagated without any antiherpetic agents and al ... | 1988 | 2838126 |
| multicentric intraepithelial neoplasia involving the vulva. clinical features and association with human papillomavirus and herpes simplex virus. | sixteen of 46 patients (35%) with grade 3 vulvar intraepithelial neoplasia (vin 3) were found to have an additional site of lower genital tract squamous cell neoplasia, primarily in the cervix. the frequency of multicentricity decreased significantly with age. in addition, patients with multicentric disease (involving the vagina and/or cervix in addition to the vulva) had a significantly higher frequency of multifocal disease involving the vulva (involving more than one location on the vulva) an ... | 1988 | 2844383 |
| inducible expression of herpes simplex virus type 2 glycoprotein gene gg-2 in a mammalian cell line. | the gg-2 glycoprotein gene of herpes simplex virus type 2 (hsv-2) was cloned into the mammalian expression vector pmsg under the control of the inducible mouse mammary tumor virus promoter. transfection of this cloned gg-2 construct into nih 3t3 cells resulted in the stable expression of gg-2 upon induction with dexamethasone. in addition, the 104,000-molecular-weight (104k) and 72k gg-2 precursors as well as the 34k secreted component were generated in the transformed cells. the synthesis of gg ... | 1988 | 2843667 |
| induction of cervical intraepithelial neoplasia in balb/c mice by uv-inactivated herpes simplex virus type 2. | 1988 | 2845906 | |
| effectiveness of a procedure for disinfecting the vaginal photoplethysmograph contaminated with herpes simplex virus type 2. | to test the effectiveness of a disinfecting procedure involving 2% glutaraldehyde, a vaginal photoplethysmograph was contaminated with a known amount of herpes simplex type 2 (hsv-2). the vaginal photoplethysmograph was then put through the disinfection procedure. two virus solutions were tested, one designed to approximate the concentration found in a naturally occurring infection (low inoculum), the other with 100 times more virus (high inoculum). varying lengths of exposure to glutaraldehyde ... | 1988 | 2844137 |
| combined herpes simplex virus type 2 and human papillomavirus type 16 or 18 deoxyribonucleic acid leads to oncogenic transformation. | tumorigenic transformation was produced by combining herpes simplex virus type 2 and human papillomavirus type 16 or 18 deoxyribonucleic acid (each is associated with cervical carcinoma). recombinant human papillomavirus type 16 and 18 deoxyribonucleic acid each containing a head-to-tail dimer of the full-length human papillomavirus respective genomes, induced morphologic transformation in an immortalized cell line (ae), obtained by transfection of syrian hamster embryo cells with immortalizing ... | 1988 | 2847531 |
| human papillomavirus & herpes simplex virus type 2 dna sequences with chronic cervicitis & invasive cervical cancer. | 1988 | 2848763 | |
| electron microscopic localization of acridine orange chromatin interaction products in cells transformed by herpes simplex virus type 2. | the purpose of this study was to examine the distribution pattern of acridine orange (ao) binding to dna in transformed cells and derived tumor cells. two cell clones from hamster embryonic fibroblasts (hef) transformed by herpes simplex virus type 2, tumor cells derived from the transformed cell clones, and normal hef in culture were processed for ultracytochemistry. uptake studies and autoradiography using [3h] thymidine and [3h]-uridine were also performed. ultrastructural examination reveale ... | 1988 | 2851256 |
| presence of insulin binding sites on viral particles. | using a standard radio-receptor assay, we have demonstrated that [125i]insulin can bind specifically to each of two types of purified enveloped viruses, influenza a virus and rous sarcoma virus. a non-enveloped icosahedral virus (echovirus 11) and herpes simplex virus type 2, which acquires its envelope from the nuclear membrane of the cell, did not possess insulin receptor activity. displacement of specifically bound radiolabelled insulin from the viral surface was achieved by addition of an ex ... | 1988 | 2851292 |
| [seroepidemiological survey of herpes simplex virus type 2 in cervical erosion women]. | 1988 | 2852541 | |
| the aetiological relationship between herpes simplex virus type 2 and carcinoma of the cervix: an unanswered or unanswerable question? | after two decades of investigation the proposed aetiological link between herpes simplex virus type 2 (hsv2) and human carcinoma of the cervix remains unproved. the initial seroepidemiological studies, which were of a retrospective nature, were in favour of the aetiological relationship, whereas the more recent prospective studies suggest that hsv infection is a co-variable. the detection of hsv genes and their products in tumour tissues has been plagued by problems of cross reactivity, but the ... | 1988 | 2853997 |