immunological reactivity of human sera with individual herpes simplex proteins: a comparative study of sera from patients with preinvasive or invasive cervical cancer and from controls.forty-three human sera collected from patients with preinvasive or invasive cervical carcinoma were analyzed for their repertoire of herpes simplex virus (hsv) specific antibodies reactive with individual viral hsv-1 and hsv-2 proteins. the reactivity was compared to that of sera from 27 control persons. the patients and controls were clinically and histologically characterized in a previous study we carried out, where the analysis of the hla-antigen pattern was compared among the groups [vass-s ...19863005489
characterization of the genes encoding herpes simplex virus type 1 and type 2 alkaline exonucleases and overlapping proteins.a detailed sequence analysis of the herpes simplex virus type 1 (hsv-1) and hsv-2 dna encoding the alkaline exonuclease mrna clusters has been completed. three partially colinear mrnas (2.3, 1.9, and 0.9 kilobases) are completely encoded within the dna sequence presented. the putative promoter regions of the transcripts were inserted upstream of a plasmid-borne chloramphenicol acetyl transferase (cat) gene and assayed for their ability to induce transcription of the cat gene upon low multiplicit ...19863005609
pathogenicity in mice of herpes simplex virus type 2 mutants unable to express glycoprotein c.herpes simplex virus type 2 (hsv-2) mutants that were unable to express glycoprotein c (gc-2) were isolated. deletions were made in a cloned copy of the gc-2 gene, and recombinant viruses containing these deletions were screened by using an immunoreactive plaque selection protocol. the viruses did not display a syncytial phenotype. intravaginal inoculation of balb/cj mice with one of the hsv-2 gc-2- viruses produced local inflammation followed by a lethal spread of the viral infection into the n ...19863005656
effects of mercury (ii) compounds on the activity of dutpases from various sources.the deoxyuridine triphosphate nucleotidohydrolases (dutpases, ec from escherichia coli k-12-,acholeplasma laidlawii b-pg9-, human kb cell-, and the herpes simplex virus (hsv) type 1- and 2-induced dutpases were purified and used to determine the effect of various mercury (ii) compounds on their activities. mercuric acetate, 5-mercuri-dutp (hgdutp), and 5-mercuri-dctp (hgdctp) acted as irreversible active site-directed inhibitors of the dutpases purified from eukaryotic organisms but no ...19863005836
infections with herpes simplex viruses (1). 19863005858
dna amplification and neoplastic transformation mediated by a herpes simplex dna fragment containing cell-related sequences.the transforming potential of the herpes simplex virus type 2 (hsv-2) bamhi fragment e (map position 0.533-0.583) encoding the 140-kda ribonucleotide reductase was assayed by transfection in established rat-2 cells. foci of refractile, morphologically distinguishable cells were induced at lower efficiency and after a longer incubation period as compared to the human tumor oncogene ej-ha-ras. focus-derived bamhi fragment e-transformed cell lines formed medium-to-large (0.1-0.25 mm) colonies in so ...19863006067
acyclovir prophylaxis in bone marrow transplant recipients.forty-two patients undergoing bone marrow transplantation were included in a randomised, double-blind and placebo controlled trial of prolonged acyclovir prophylaxis against infections with viruses of the herpes group. twenty patients were allocated to receive acyclovir and 22 to receive placebo. acyclovir or placebo was administered i.v. at a dose of 250 mg/m2 twice daily, starting 5 days before transplantation. at 5 weeks after transplantation, administration was changed to tablets, 400 mg thr ...19853006228
virological aspects on herpesvirus infections.the family herpesviridae include five important human pathogens, herpes simplex virus types 1 and 2 (hsv-1, hsv-2), varicella-zoster virus (vzv) and cytomegalovirus (cmv). the five viruses are represented in all three herpesvirus subfamilies, a subdivision based on differences in phenotypic properties such as length of replication cycle, cytotoxic properties and host cell range. the herpesviruses have dna genomes sharing characteristic anatomical features. they replicate in the nuclei of eukaryo ...19853006235
identification, properties, and gene location of a novel glycoprotein specified by herpes simplex virus 1.we report the identification of a novel herpes simplex virus 1 (hsv-1) glycoprotein reactive with type specific monoclonal antibody h1379. the monoclonal antibody reacted with two broad bands with apparent mol wt of 60k to 68k and 44k to 48k formed by infected cell lysates subjected to electrophoresis in denaturing polyacrylamide gels and electrically transferred to a nitrocellulose sheet. early in infection the h1379 reactive protein was found in the faster migrating band. the rate of accumulat ...19863006335
comparison of risk factors for cervical cancer in different populations.the incidence of cervical cancer has been found to vary between populations. risk factors of cervical cancer include early age at first marriage, multiple marriages and antibodies to herpes simplex virus type 2 (hsv-2). the interrelatedness of these risk factors was examined by comparing data collected from 428 cancer cases and 947 control women selected from 6 populations having standardized cervical cancer incidence rates varying from 9.3 to 85.1 per 100,000. logistic regression analysis revea ...19863007372
characterization of a herpes simplex virus type 2-specified glycoprotein with affinity for n-acetylgalactosamine-specific lectins and its identification as g92k or gg.extracts from herpes simplex virus type 2 (hsv-2)-infected cells were subjected to affinity chromatography with gel-bound helix pomatia lectin (hpa). only one hsv-2-specified glycoprotein was isolated by this procedure and the glycoprotein had an apparent molecular weight of 130 000 (130k). the hpa-binding glycoprotein was genetically mapped, using hsv-1 x hsv-2 intertypic recombinants into the short component of the hsv-2 genome. the mapping position, electrophoretic mobility and the antigenic ...19863007660
novel herpes simplex virus type 1 glycoproteins identified by antiserum against a synthetic oligopeptide from the predicted product of gene us4.gene us4 of herpes simplex virus type 1 (hsv-1) has been predicted, from dna sequence analysis, to encode a protein of molecular weight 25237 and its properties suggest it to be a membrane-associated protein. we have investigated this protein by raising antiserum to a synthetic oligopeptide corresponding to a stretch of amino acids from an internal hydrophilic region of the predicted sequence. this antiserum immunoprecipitates three glycoprotein species of apparent mol. wt. 37 000, 48 000 and 56 ...19863007661
herpes simplex virus type 1 dna sequences which direct spread of virus from cornea to central nervous system.the virulence of a herpes simplex virus (hsv) intertypic recombinant possessing hsv-1 dna sequences from map units 0.31 to 0.44 and hsv-2 sequences from map units 0 to 0.30 and 0.45 to 1.0 were compared with the virulence of the two parental strains. following ocular inoculation, both the intertypic recombinant and the hsv-1 parent replicated at the infection site and spread to the peripheral and central nervous system (cns) to produce fatal encephalitis. the hsv-2 parent also replicated at the ...19863008422
efficacy of 5-methoxymethyl-2'-deoxyuridine in combination with arabinosyladenine for the treatment of primary herpes simplex genital infection of mice and guinea pigs.the relative efficacy of 5-methoxymethyl-2'-deoxyuridine (mmdurd), arabinosyladenine (ara-a) and the combination of mmdurd and ara-a in the treatment of experimental genital herpes (gh) was investigated using mouse and guinea pig models. the infection was initiated by intravaginal inoculation using either hsv-2, strain x-265 or hsv-2, strain ms. treatment was initiated 3 h post virus inoculation. the parameters used to evaluate efficacy were: percent mortality; mean day of death; virus yield fro ...19863008644
antibody response to the herpes simplex virus type 2 ag-4 antigen in guinea pigs with genital herpes infections.female guinea pigs, inoculated intravaginally with a herpes simplex virus-type 2 (hsv-2) strain m1, developed typical symptoms of a primary and recurrent genital herpes infection. sera from hsv-2 infected guinea pigs taken during the primary or recurrent stages of the genital infection contained complement (c)-fixing antibody which reacted with an apparent type specificity to an early 4 h hsv-2 infected cell extract (ag-4) when compared to a 4 h hsv-1 infected cell extract. this c-fixing anti-ag ...19863008691
[demonstration of latent infection by herpes simplex virus type 2 (hsv-2) in the spinal cord of the guinea pig]. 19853008964
[inhibitory effect of tai-ding-an on dna and protein synthesis of herpes simplex virus type 2 (hsv-2)]. 19853008965
intracellular localization and serological identification of a hsv-2 protein in cervical cancer.exfoliated atypical cells from us and italian patients with cervical intraepithelial neoplasia or invasive cancer stained in indirect immunofluorescence with a monoclonal antibody to a high mw hsv-2 protein (icp10). the proportion of staining cells increased as a function of the severity of the cervical anaplasia. in the majority of cases with mild or moderate dysplasia staining was restricted to the cytoplasm while atypical cells from 6 of 7 cases with marked dysplasia and 7 of 7 cases with inv ...19863009194
genital reinfection after recovery from initial genital infection with herpes simplex virus type 2 in guinea pigs.the natural history of genital reinfection with herpes simplex virus type 2 (hsv-2) three weeks to one year after initial infection was explored by using a guinea pig model. intravaginal reinoculation with hsv-2 of animals that had recovered from initial genital infection produced by wild-type or thymidine kinase-deficient hsv-2 produced asymptomatic genital reinfection characterized by high-titered replication of hsv-2 in the vaginal vault. reinoculation did not result in acute neural infection ...19863009643
synthesis and antiviral properties of 5-(2-substituted vinyl)-6-aza-2'-deoxyuridines.the following 5-(2-substituted vinyl)-6-aza-2'-deoxyuridines were synthesized: (e)-5-(2-bromovinyl) (2) (6-aza-bvdu), 5-(2-bromo-2-fluorovinyl) (a mixture of e and z isomers) (3), (e)-5-(2-chlorovinyl) (4), (e)-5-[2-(methylthio)vinyl] (5), 5-(2,2-dibromovinyl) (6), and 5-(3-furyl) (7). the synthesis of 2-6 utilized wittig-type reactions on 5-formyl-1-(2'-deoxy-3', 5'-di-o-p-toluoyl-beta-d-erythro-pentofuranosyl)-6-azauracil (16). 6-aza-bvdu (and its alpha-anomer) was also synthesized from (e)-5- ...19863009815
cloning and characterization of oril2, a large palindromic dna replication origin of herpes simplex virus type origin of replication within the long unique sequence of herpes simplex virus type 2 designated oril2 has been identified in a position homologous to its type 1 counterpart, oril1, between map coordinates 0.398 and 0.413. the difficulties encountered in previous attempts to clone both oril2 and oril1 in an undeleted form were surmounted by minimizing the growth of the host escherichia coli, using a recbc sbcb e. coli host, and purifying the full-length plasmid from delected forms by using a n ...19863009865
expression of herpes simplex virus glycoproteins in polarized epithelial cells.members of the herpesvirus family mature at inner nuclear membranes, although a fraction of the viral glycoproteins is expressed on the cell surface. in this study, we investigated the localization of herpes simplex virus type 2 (hsv-2) glycoproteins in virus-infected epithelial cells by using a panel of monoclonal antibodies directed against each of the major viral glycoproteins. all of the hsv-2 glycoproteins were localized exclusively on the basolateral membranes of vero c1008, madin-darby bo ...19863009881
identification by antibody to a synthetic peptide of a protein specified by a diploid gene located in the terminal repeats of the l component of herpes simplex virus the course of studies on the a sequences located at the termini of and at the junction between the l and s components of herpes simplex virus 1 dna, j. chou and b. roizman (j. virol. 57:629-637, 1986) noted that the a sequence acted as a gamma 1 promoter when fused to the structural sequence of the thymidine kinase gene, the b inverted repeat sequences located in the l component next to the a sequences contained an open reading frame predicted to encode the protein of 358 amino acids with a m ...19863009891
effect of delta 9-tetrahydrocannabinol on herpes simplex virus type 2 vaginal infection in the guinea pig.the present investigation was undertaken to determine whether delta 9-tetrahydrocannabinol (delta 9-thc) decreases host resistance to herpes simplex virus type 2 vaginal infection in the guinea pig. the guinea pig was selected as the host since it has been shown to express a spectrum of primary herpes genitalis which is similar to that in humans. animals were administered delta 9-thc or vehicle intraperitoneally on days 1-4, 8-11, and 15-18. herpes simplex virus was introduced intravaginally on ...19863010327
recurrent genital herpes and suppressive oral acyclovir therapy. relation between clinical outcome and in-vitro drug evaluate the association between in-vitro resistance of herpes simplex virus type 2 to acyclovir and breakthrough recurrences of herpes despite chronic suppressive therapy, we determined the in-vitro sensitivity of herpes simplex virus isolated before, during, and after therapy. one hundred eighty-three virus isolates from 107 patients were tested. before therapy, the median amount of drug required to inhibit 50% of the virus in tissue culture (id50) was 0.91 microgram/ml. the median id50 aft ...19863010782
plaque autoradiography assay for the detection and quantitation of thymidine kinase-deficient and thymidine kinase-altered mutants of herpes simplex virus in clinical isolates.a plaque autoradiography assay to detect and quantitate thymidine kinase (tk) mutants of herpes simplex virus type 1 (hsv-1) and hsv-2 in clinical samples is described. this method utilizes the selective incorporation of [125i]iododeoxycytidine, a pyrimidine analog selectively phosphorylated by the hsv tk. only cells infected with tk-competent virus will efficiently incorporate iododeoxycytidine and are the only cells detected by autoradiography. furthermore, this assay discriminates between tk+ ...19853010836
selective in vitro and in vivo activities of 5-(2-haloalkyl)pyrimidine nucleoside analogs, particularly 5-(2-chloroethyl)-2'-deoxyuridine, against herpes simplex virus.5-(2-chloroethyl)-2'-deoxyuridine (cedu), 5-(3-chloropropyl)-2'-deoxyuridine (cpdu), and 5-(2-chloroethyl)-2'-deoxycytidine (cedc) were evaluated for activity against herpes simplex virus type 1 (hsv-1) and hsv-2 in vitro. their mics for hsv-1 in primary rabbit kidney cell cultures were 0.15, 0.20, and 0.60 micrograms/ml, respectively; their mics for hsv-2 were about 10-fold higher. when tested in parallel, the reference compounds 5-ethyl-2'-deoxyuridine, 5-iodo-2'-deoxyuridine, acyclovir, and ( ...19853010841
mechanism of selective inhibition of human cytomegalovirus replication by 1-beta-d-arabinofuranosyl-5-fluorouracil.four kinds of 1-beta-d-arabinofuranosyl-5-halogenouracil were examined for inhibition of human cytomegalovirus (hcmv) and herpes simplex virus type 1 (hsv-1) and 2 (hsv-2) replication. 1-beta-d-arabinofuranosyl-5-fluorouracil (ara-fu) was the most effective against hcmv, whereas 1-beta-d-arabinofuranosyl-5-bromouracil was the most effective against hsv-1 and hsv-2. the mechanism of action of ara-fu on hcmv replication was also studied. the dttp pool size in human embryonic fibroblasts was increa ...19853010844
molecular cloning of herpes simplex virus type 2 dna.restriction enzyme hindiii digestion of the whole genome of herpes simplex virus type 2 strain 186 yielded 10 dna fragments with molecular weights ranging from approximately 22 x 10(6) to 1.2 x 10(6), which were cloned into the hindiii site of bacterial plasmid pacyc 184. the cloned fragments were identified by hybridization to hsv-2 virus dna and by double digestion with restriction endonucleases. the recombinant plasmids, even if they carried dna sequences with molecular weights of more than 1 ...19863011764
acute genital infection in guinea pigs: effect of recombinant interleukin-2 on herpes simplex virus type 2.human recombinant interleukin-2 (ril-2) modifies infection with herpes simplex virus type 2 (hsv-2) in normal guinea pigs. animals were injected sc with ril-2 twice a day, beginning 24 hr before infection and continuing for three subsequent days. guinea pigs were assigned to five different regimens in which ril-2 was administered daily at dosages from 8 x 10(3) u/kg to 8 x 10(5) u/kg. after intravaginal inoculation with hsv-2, 83% of 24 control animals developed apparent genital herpes, and 5% h ...19863011918
suppression and enhancement of humoral antibody formation by herpes simplex virus types 1 and 2.intraperitoneal infection of mice and rats by herpes simplex virus type 2 (hsv-2) but not type 1 (hsv-1) resulted in suppression of antibody formation on subsequent challenge with hsv-1 or hsv-2. application of silica considerably enhanced antibody formation after primary hsv-1 infection, but only slightly after primary hsv-2 infection. suppression induced by hsv-2 was, however, reduced significantly by injection of silica 21 days later, on the day of the second injection of hsv-2. suppression c ...19863011967
x-linkage of the early in vitro alpha/beta interferon response of mouse peritoneal macrophages to herpes simplex virus type 2.the genetics of the early interferon response of mouse peritoneal cells to infection with herpes simplex virus type 2 (hsv-2) was studied in susceptible balb/c and more resistant c57bl/6 mice and in reciprocal crosses between these mice. wash-outs of the peritoneal cavity of normal c57bl/6 mice contained significantly more cells than wash-outs from balb/c mice. therefore, interferon induction with hsv-2 was studied under standardized conditions in vitro. peritoneal cells reacted to hsv-2 infecti ...19863011968
the herpes simplex virus type 2 alkaline dnase activity is essential for replication and growth.a mutant of herpes simplex virus type 2 (hsv-2), which is temperature-sensitive (ts) for the induction of an alkaline dnase activity, was examined at a number of different temperatures. induction of dnase activity by this mutant resembled that of wild-type (wt) virus at 31 degrees c but was greatly reduced at 38.5 degrees c and barely detectable at 39.2 degrees c. virus dna synthesis showed similar patterns, exhibiting wt levels at 31 degrees c, reduced levels at 38.5 degrees c and very little i ...19863011973
inhibition of herpes simplex virus-induced dna polymerases and cellular dna polymerase alpha by triphosphates of acyclic guanosine analogs.the triphosphates of the antiherpes acyclic guanosine analogs (r)- and (s)-enantiomers of 9-(3,4-dihydroxybutyl)guanine [bcvtp and (s)-dhbgtp], 9-(4-hydroxybutyl)guanine (hbgtp), and 9-(2-hydroxyethoxymethyl)guanine (acvtp) were investigated for their effects on partially purified dna polymerases of herpes simplex virus type 1 and type 2 (hsv-1 and hsv-2) as well as cellular dna polymerase alpha of calf thymus and vero cells. the triphosphates of the four analogs were all competitive inhibitors ...19863012321
specific inhibition of herpesvirus ribonucleotide reductase by synthetic peptides.ribonucleotide reductase is an essential enzyme for dna synthesis in all prokaryotic and eukaryotic cells; it catalyses the reductive conversion of ribonucleotides to deoxyribonucleotides. several herpesviruses including herpes simplex virus type 1 (hsv-1), hsv-2, pseudorabies virus (prv), equine herpesvirus type 1 (ehv-1) and epstein-barr virus (ebv) have been found to induce novel ribonucleotide reductase activities. there is evidence that the hsv-1 ribonucleotide reductase activity is virus-e ...19863012359
analysis of dna from recurrent genital herpes simplex virus isolates by restriction endonuclease a blind study, dna from 40 clinical isolates of herpes simplex viruses was analyzed by restriction endonucleases to determine whether serial isolates from an individual patient could be identified and whether exogenous reinfection occurred within this population. five of the 40 isolates served as controls. based on restriction patterns obtained following bam h1 cleavage of dna, 35 isolates were assigned to 15 patients. isolates from two patients displayed variation in the electrophoretic mobi ...19863012807
cellular immunity to vaccinations and herpesvirus infections after bone marrow transplantation.the cellular immune response to herpesviruses was studied in 46 recipients of marrow grafts (23 autologous, 23 allogeneic). that study was performed in vitro by evaluating the degree of lymphocyte proliferative responses to herpes simplex virus (hsv), cytomegalovirus (cmv), and varicella zoster virus (vzv). no primary infections with any of those viruses were noted after bone marrow transplantation (bmt). the incidence of active infection in seropositive patients was significantly lower after au ...19863012833
efficacy of the acyclic guanosine analog buciclovir [(r)-9-(3,4-dihydroxybutyl)guanine] in experimental genital herpes.the efficacy of the anti-herpesvirus drug buciclovir [(r)-9-(3,4-dihydroxybutyl)guanine] was investigated in guinea pigs and mice infected intravaginally with herpes simplex virus type 2. topical treatment initiated early after infection was efficacious, in contrast to topical treatment delayed 24 h or more. systemic treatment of infected mice could not prevent the spread of virus to the brain and mortality. systemically administered buciclovir had an effect in guinea pigs, even after delayed on ...19863013082
comparison of susceptibilities of varicella-zoster virus and herpes simplex viruses to nucleoside analogs.the susceptibilities of varicella-zoster virus (vzv) and herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) to 17 nucleoside analogs were compared by a plaque reduction assay with human embryonic lung fibroblast cells. the susceptibility of vzv to certain nucleoside analogs was different from that of hsv-1. against vzv the 5-halogenovinyl-arabinosyluracils were the most potent of the compounds tested.19863013088
evaluation of five cell types for the isolation of herpes simplex virus.five cells were evaluated in a comparative analysis for sensitivity, specificity, and rapidity in detecting the presence of herpes simplex virus hsv-1 and hsv-2. included in this study were human embryonic kidney (hek), rabbit kidney (rk), mrc-5, mink lung (ml), and microtus agrestis (umma). a total of 274 specimens from genital, throat, skin, or other sources that were submitted for hsv isolation were used in the study. the sensitivity of the different cells was assessed by the total number of ...19863013496
rapid identification of herpesvirus simiae (b virus) dna from clinical isolates in nonhuman primate colonies.a rapid, simple technique, based on restriction endonuclease analysis of radioactively labeled infected cell dna, is described for identification of herpesvirus simiae (b virus) infection in clinical isolates from nonhuman primates. isolates can be screened within 2-3 days from the time of collection of a specimen from a suspect lesion to final viral identification. isolates were obtained from eight animals with suspected b virus infections. the results indicated the presence of b virus in each ...19863013916
a filamentous distribution for the herpes simplex virus type 2-encoded major dna-binding protein.monoclonal antibodies reacting with the herpes simplex virus (hsv)-encoded major dna-binding protein defined an intracellular filamentous network. this network was associated predominantly with the infected cell nucleus and occurred in cells infected with hsv type 2. it did not co-distribute with microfilaments, microtubules or intermediate filaments, and dna synthesis was required for its formation. we suggest explanations for the occurrence and function of this novel filamentous network struct ...19863014046
characterization of the tumor-associated 38-kd protein of herpes simplex virus type 2.the bglii n dna fragment of herpes simplex virus type 2 (hsv 2), which is capable of oncogenically transforming cells in vitro, encodes a 37,800-dalton (38-kd) protein that has been seroepidemiologically associated with uterine cervical carcinoma. polyclonal monospecific antiserum was produced against electrophoretically purified 38 kd from hsv 2-infected cells and used to identify antigenic and biochemical characteristics of the protein as well as to probe transformed cells for the expression o ...19863014138
comparative studies of host-cell reactivation, cellular capacity and enhanced reactivation of herpes simplex virus in normal, xeroderma pigmentosum and cockayne syndrome reactivation (hcr) of uv-irradiated herpes simplex virus type 2 (hsv-2), capacity of uv-irradiated cells to support hsv-2 plaque formation and uv-enhanced reactivation (uver) of uv-irradiated hsv-2 were examined in fibroblasts from 4 patients with cockayne syndrome (cs), 5 with xeroderma pigmentosum and 5 normals. all uv-survival curves for hsv-2 plaque formation showed 2 components. hcr was similar to normal for the xp variant strain and the 2 cs strains tested, but substantially redu ...19863014327
[comparative study of the effectiveness of acycloguanosine and phosphonoacetic acid in herpetic infection in cell cultures].a comparative study of cytotoxicity and antiherpes activity of acycloguanosine (acg) of the soviet and american manufacture and a soviet preparation of phosphonoacetic acid (paa) for herpes simplex virus types 1 and 2 (hsv-1 and hsv-2) was carried out in primary and continuous cell cultures. the acg preparation was shown not to be inferior in maximal tolerated concentration, minimal inhibiting concentration, and chemotherapeutic index (cti) to american drug. zovirax (zvr), in relation to both he ...19863014751
activities of 1-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)-5-iodocytosine and its metabolites against herpes simplex virus types 1 and 2 in cell culture and in mice infected intracerebrally with herpes simplex virus type measured by plaque and yield reduction assays, several metabolites of 1-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)-5-iodocytosine (fiac) were highly active against herpes simplex virus types 1 and 2. these metabolites included the 2'-deoxy-2'-fluoroarabinosyl derivatives of 5-iodouracil (fiau), cytosine (fac), uracil (fau), and thymine (fmau). in mice inoculated intracerebrally with herpes simplex virus type 2, the relative order of potency of these compounds and licensed antiviral drugs was ...19863015003
effect of interferon on herpes simplex virus replication in murine macrophage-like cell lines.the effect of interferon on the replication of herpes simplex virus types 1 and 2 was studied in two murine macrophage-like cell lines which differ in their ability to synthesize interferon. higher titers of herpes simplex virus type 1 occurred in pu5-1.8 cell cultures where interferon was not produced than in j774a.1 cell cultures where low amounts of interferon were produced. herpes simplex virus type 2 replicated poorly in both types of cell cultures. interferon synthesis was induced in j774a ...19863015020
herpes simplex virus type 1 and 2 in the adrenal glands: replication and histopathology.the adrenal glands were shown to be the most severely infected organs in the early phase of hsv-1 infections (up to 10 days p.i.) after i.p. infections in mice. virus could be isolated from the adrenal glands as early as one hour after infection with pathogenic and apathogenic strains. infection of the adrenal glands is a result of viremia. the content of hsv-1 (5 strains) was much higher in the adrenals than in spleen and liver. it peaked at 3-4 days p.i. compared to 1-2 days in spleen and live ...19863015081
a latent infection of herpes simplex virus type 2 in a human neuroblastoma cell line imr-32.human neuroblastoma (imr-32) cells were infected with herpes simplex virus type 2 (hsv-2) at a multiplicity of infection (moi) of 2 plaque-forming units (pfu)/cell and were cultured at 40 degrees c for 14 days. then neither infectious virus particles nor virus capsids were detected in these cells whereas the presence of virus-specific antigens was observed by immunofluorescent antibody staining technique in 16.9 +/- 3.2 per cent of the infected cell population. when the cultivation temperature w ...19863015082
transformation of dog embryo kidney cells by human herpesviruses.the infection of dog embryo kidney (dek) cells with herpes simplex virus type 2 (hsv-2) or human cytomegalovirus (hcmv) led to the development of transformed cell lines. rapidly dividing dek cells with unlimited division potential exhibited growth in 2% serum, contained nuclear virus antigens, and formed small (+/- 0.2 mm) colonies in 0.3% agarose. immortal cell lines showing the same transformation properties were also obtained after transfection with purified hsv-2 or hcmv dna. these results c ...19863015422
[discovery of herpes simplex virus (hsv)-like particles in biopsy samples of cervical carcinoma].the tissue of cervical cancer and hsv-2 infected baby rabbit kidney (brk) cells were studied comparatively by electron microscope. in 2 out of 12 samples from patients with cervical carcinoma, some virus-like particles were observed in the cytoplasm or intercellular space. some particles seemed to be enveloped by endoplasmic reticulum. according to their size, structure and morphology, these particles resembled the herpes simplex virus. the relationship between the discovery of herpes simplex vi ...19853015529
purulent penile ulcers of patients in 80 patients with painful purulent penile ulcers who attended the outpatient service of middle road hospital in singapore, haemophilus ducreyi was isolated from 18 (22%), herpes simplex virus type 2 from nine (11%), and neisseria gonorrhoeae from eight (10%). primary pathogens were not isolated from 45 (57%) men. painful purulent penile ulcers were more common in uncircumcised men, and patients had often acquired the disease after sexual intercourse with prostitutes in singapore.19863015775
prospective study on the relationship between cervical neoplasia and herpes simplex type 2 virus. iii. presence of herpes simplex type-2 antibody in sera of subjects who developed cervical neoplasia later in the study.paired sera taken from 63 subjects who developed cervical neoplasia in the course of a prospective study on this disease were examined for the presence of herpes simplex virus type 2 (hsv-2) antibody. the first serum was taken at enrollment into the study, while the second was obtained after development of the disease, i.e. 2-4 years later. simultaneously, paired sera from a group of control subjects, who remained free of any cytological and colposcopical abnormalities throughout the study, were ...19863015805
antibody response, recurrence patterns and subsequent herpes simplex virus type 2 (hsv-2) re-infection following initial hsv-2 infection of guinea-pigs: effects of acyclovir.the production of antibody to specific herpes simplex virus type 2 (hsv-2) polypeptides, the recurrence patterns and the susceptibility to re-infection were studied in the guinea-pig model of genital hsv-2 infection. further, we defined the effects of acyclovir (acv) therapy on these parameters of infection. treatment with acv reduced the clinical severity of the initial disease but did not affect vaginal viral shedding. production of neutralizing antibody as well as antibody to the nucleocapsid ...19863016155
asymptomatic virus shedding in men with genital herpes infection.13 men with a history of recurrent genital herpes simplex virus type 2 (hsv-2) infection were followed daily for 4 weeks with samples taken from the urethra for virus isolation. asymptomatic virus shedding occurred in 5 men who had 1 single positive isolation each. four of these urethral isolates were typed as hsv-1 and 1 as hsv-2.19863016883
a rapid detection of herpes simplex virus by elisa in asymptomatic pregnant women and neonates.herpes simplex virus (hsv) was detected by the enzyme-linked immunosorbent assay (elisa). the assay system employed rabbit anti-hsv-2-coated microplates to detect hsv in clinical specimens and the same reagent labelled with peroxidase as a conjugate. the hsv type 2 obtained from vero cell culture and normal cell lysate (ncl) were used as positive and negative reference antigens respectively. hsv was detected in 40 (9.93%) of vaginal swabs obtained from 403 pregnant women just before the deliveri ...19863016912
the herpes simplex virus type 2 equivalent of the herpes simplex virus type 1 us7 gene and its flanking sequences.nucleotide sequencing studies (d. j. mcgeoch, a. dolan, s. donald, and f. rixon, 1985, j. mol. biol. 181, 1-14) have indicated that herpes simplex virus type 1 (hsv-1) has a coding sequence, referred to as us7, between the genes for the glycoproteins d and e (gd and ge). northern blot analysis and nucleotide sequencing have been carried out to show that the type 2 virus (hsv-2) has an equivalent to the us7 gene. a comparison with the hsv-1 sequence has revealed some surprising similarities and d ...19863016980
[isolation of an endogenous guinea pig herpeslike virus from hartley guinea pigs with latent herpes simplex virus type 2 infection]. 19853017012
herpes-related polypeptides from a human cervical carcinoma cell line.using antiserum against herpes simplex virus type 2 (hsv-2) infected cells, eight polypeptides with similar molecular weights could be immunoprecipitated from the nearly diploid, human cervical carcinoma cell line c4ii and from hsv-2 transformed hamster and mouse cells. only two of these polypeptides corresponded to those from hsv-2 infected cells, including the putative hsv-2 transformation-related 35k protein. partial proteolytic cleavage products of the immunoprecipitated 35k polypeptides fro ...19863017554
cryosurvival of herpes simplex virus-2 during cryopreservation of human spermatozoa.herpes simplex virus (hsv) is a sexually transmitted agent which has potential association with cervical cancer, as well as with high morbidity and mortality in perinatal infection. its incidence is estimated just after gonorrhea and chlamydia trachomatis infections. cryobanking of human semen is accepted worldwide in therapeutic use for both husband and donor. serious consequences could follow the clinical applications of cryobanked human semen in insemination, if some ejaculates contain hsv an ...19863017635
failure to induce cervical cancer in mice by long-term frequent vaginal exposure to live or inactivated herpes simplex viruses.c57 mice aged 8-10 weeks in groups of 50 each received vaginal cotton pellets soaked in lysates of hep-2 cells, either mock-infected or infected with herpes simplex virus i, herpes simplex virus 2, and highly attenuated recombinant viruses 5 times a week for 89 to 114 weeks. an untreated group was also included. the mock-infected and some of the infected cell lysates were exposed to ultraviolet light at a dose sufficient to inactivate virus. smears of exfoliated vaginal cells collected once a mo ...19863017867
analyses of transplanted murine tumors for hsv dna sequences.meignier et al. (1986) report the results of exposure of c57bl/6ncr mice to vaginal plugs containing live or inactivated herpes simplex virus 1 or 2 (hsv-1 or hsv-2) or recombinant viruses 5 times a week for up to 114 weeks. genital organs showing abnormalities were transplanted into nude mice. of 33 transplants, 13 produced subcutaneous tumors in nude mice and 12 were subsequently transplanted into c57bl/6ncr mice. we report that the dna extracted from coded tumor tissues of nude mice and from ...19863017868
zosteriform spread of herpes simplex virus type 2 genital infection in the guinea-pig.a model of herpes simplex virus type 2 (hsv-2) infection was developed in the guinea-pig that permits investigation of the role of neural spread of virus in the pathogenesis of genital skin disease. after hsv-2 inoculation of an abraded area lateral to the external genital skin, virus was first detected in the ipsilateral (to the inoculation site) peripheral nerves or genital skin on day 2 or 3 post-inoculation followed by detection in the ipsilateral dorsal root ganglia and spinal cord on day 3 ...19863018127
genetically determined difference in the antiviral action of alpha/beta interferon in cells from mice resistant or susceptible to herpes simplex virus type 2.resistance of mice to infection with herpes simplex virus type 2 (hsv-2) is genetically determined. embryonic cells from susceptible balb/c and resistant c57bl/6 mice were equally sensitive to infection with hsv-2 as judged by plaque area, plaquing efficiency, endpoint titration and virus yield. cells from c57bl/6 mice showed a higher sensitivity than cells from balb/c mice to the protective action of two preparations of alpha/beta interferon against challenge with hsv-2. this was evident both f ...19863018128
delta-9-tetrahydrocannabinol enhances release of herpes simplex virus type 2.this study was undertaken to determine the effect of micromolar concentrations of delta-9-tetrahydrocannabinol (delta-9-thc) on herpes simplex virus type 2 (hsv-2) replication in vitro. virus-infected vero cells pretreated for 24 h with 10(-5) m- or 10(-6) m-delta-9-thc yielded 100-fold increases in infectious extracellular virus. transmission electron microscopy of drug-treated cells revealed plasma membrane dissolution, distension of the smooth and rough endoplasmic reticulum, and the appearan ...19863018134
carbocyclic analogues of 5-halocytosine nucleosides.carbocyclic analogues of 5-halocytosine nucleosides were prepared by direct halogenation of the carbocyclic analogues of cytidine, 2'-deoxycytidine, 3'-deoxycytidine, or ara-c. the 5-chloro and 5-bromo derivatives of the cytidine (carbodine) and of the 2'-deoxycytidine analogues and the 5-iodo derivatives of all four of the cytosine nucleoside analogues were prepared. all of the c-5-halocytosine nucleosides, as well as the parent c-cytosine nucleosides, were tested against a strain of herpes sim ...19863018246
herpes simplex virus suppression of human endothelial matrix protein synthesis is independent of viral protein synthesis.protein synthesis was determined in cultures of human umbilical cord vein endothelial cells (ec) infected with either herpes simplex type 1 (hsv-1) or type 2 (hsv-2). monolayers were infected for 1 hour with either 5 or 20 infectious virus particles per ec (multiplicity of infection of 5 or 20). at different times after infection, infected and noninfected cultures were pulsed with either 5 mu ci/ml of [14c]proline or 25 mu ci/ml of [35s]methionine for 1 or 2 hours. autoradiograms of sodium dodec ...19863018360
synergistic inhibitory effect of acyclovir and human native beta-interferon on the growth of herpes simplex virus type 2 in human embryo fibroblast cell cultures. 19863018459
antiherpetic effects of a human alpha interferon analog, ifn-alpha con1, in hamsters.the efficacy of a novel consensus form of human alpha interferon designated ifn-alpha con1 was evaluated against herpesvirus infections in vitro and in vivo. at comparable antiviral concentrations, natural lymphoblastoid ifn, ifn-alpha con1, the molecular subtype ifn-alpha, and the hybrid ifn-alpha ad(bgl) obtained by recombinant dna methods conferred similar protection against herpes simplex virus type 1 and type 2 (hsv-2) infections of human cells in vitro. whereas 7 x 10(5) u of ifn-alpha ad( ...19863019238
specific transfer factor protects mice against lethal challenge with herpes simplex virus.bovine transfer factor (tfd) specific to herpes simplex virus (hsv)1 or to hsv2 was prepared by immunizing calves with the corresponding virus. the tfd preparations were then injected into swiss mice in an attempt to protect them against a subsequent lethal challenge with hsv1 or hsv2 virus. it was thus shown that injection of anti-hsv tfd protects the mice against the corresponding hsv virus, whereas the injection of a nonspecific tfd (anti-cmv) fails to protect against a challenge with hsv1. f ...19863019568
human papillomavirus in clinically and histologically normal tissue of patients with genital study the association of human papillomavirus (hpv) and herpes simplex virus (hsv) with genital cancer, we collected specimens of cervical, vulvar, endometrial, and vaginal tumors at the time of operation in patients with cancer. in some patients, matched internal-control (histologically normal) tissue was also collected. dna extracted from the tissue was probed with radiolabeled hpv type 16 dna, hpv type 18 dna, and cloned fragments of hsv type 2 dna. hybridization to the hindiiia clone of h ...19863020404
localization and comparative nucleotide sequence analysis of the transforming domain in herpes simplex virus dna containing repetitive genetic elements.the 7.5-kilobase bamhi e fragment (bamhi-e) of herpes simplex virus type 2 (hsv-2) dna (map position 0.533-0.583) encodes the 144-kda subunit of ribonucleotide reductase and induces the neoplastic transformation of immortalized cell lines. to define the minimal transforming region of bamhi-e, a series of subclones were constructed that spanned the entire fragment. these subclones were assayed for focus formation in rat-2 cells. removal of the promoter region from the viral 144-kda-protein gene l ...19863020562
lack of evidence for intertypic recombinants in the pathogenesis of recurrent genital infections with herpes simplex virus type 1.clinical observations indicate that herpes simplex virus type 1 (hsv-1) is significantly less likely than herpes simplex virus type 2 (hsv-2) to establish latency in (or reactivate from) sacral ganglionic tissue. in an effort to identify viral functions associated with latency, we analyzed hsv-1 isolates from three patients with established recurrent genital herpes and sought evidence of dna sequences and proteins similar to those found in hsv-2. by restriction endonuclease cleavage patterns and ...19863020720
passage of herpes simplex virus type 1 on chick embryo fibroblasts confers virulence for chick embryos.the pathogenesis of chorioallantoic membrane (cam) infection with herpes simplex virus 1 and 2 (hsv-1 and hsv-2) as well as chick embryo fibroblast (cef) passaged hsv-1 was studied. it was found that hsv-2 is at least a million-fold more virulent than hsv-1 as measured by pfu/ld50 ratios for the embryo. serial passage of hsv-1 in vitro on cef cells selected for a virus (cefp10) which, unlike its parental hsv-1 strain, is able to kill the embryo. the restriction endonuclease maps of cefp10 and it ...19863020830
in vitro activity of ketoconazole against herpes simplex virus.the effects of ketoconazole alone and in combination with acyclovir and adenine arabinoside upon the replication of herpes simplex virus types 1 and 2 (hsv-1 and -2) were investigated by using a yield reduction assay. ketoconazole demonstrated antiviral activity against hsv-1 and -2 and synergistic antiviral activity when it was combined with acyclovir. combinations of ketoconazole with adenine arabinoside resulted in either interference or indifference. the effects of ketoconazole upon the prot ...19863021048
establishment of mutant murine mammary carcinoma fm3a cell strains transformed with the herpes simplex virus type 2 thymidine kinase establish cell systems appropriate for investigating the mode of action of antiherpetic nucleoside analogues, mutant cell strains were constructed from murine mammary carcinoma fm3a cells, which were deficient in tk, but were transformed with a recombinant plasmid dna containing the hsv-2 tk gene. the transformed cells incorporated the viral dna, expressed viral tk activity and showed unusually high sensitivity to the cytostatic action of the antiherpetic nucleoside analogues acv and ivdu, bo ...19863021345
elisa for detection of igg and igm antibodies to hsv-1 and hsv-2 in human sera.a rapid, enzyme-linked immunoassay (elisa) was applied to identify and measure specific igg and igm antibodies to herpes simplex viruses types 1 and 2 (hsv-1 and hsv-2). detergent solubilized infected cells and mock-infected cells were used as antigens in the assay. identification of type-specific antibodies was achieved by a competition assay in which clinical sera mixed with hsv-1 or hsv-2 antigens were assayed for reactivity to identical antigens coating wells of polystyrene microtiter plates ...19863021796
a novel glycoprotein for detection of herpes simplex virus type 1-specific antibodies.a novel herpes simplex virus type 1 (hsv-1)-specific glycoprotein reactive with monoclonal antibody h1379 was purified by affinity chromatography. this glycoprotein, provisionally designated as gg-1, forms two sets of bands with molecular weights of 40-44,000 and 60-88,000. when used in an immunodot enzymatic assay, gg-1 reacted strongly with rabbit antisera to hsv-1, but not with sera hyperimmune to hsv-2. specificity of the assay was further established by the lack of reactivity of convalescen ...19863021801
binding of complement component c3b to glycoprotein gc of herpes simplex virus type 1: mapping of gc-binding sites and demonstration of conserved c3b binding in low-passage clinical isolates.the sites on glycoprotein gc of herpes simplex virus type 1 (hsv-1) which bind complement component c3b were evaluated by using anti-gc monoclonal antibodies and mutants which have alterations at defined regions of the glycoprotein. monoclonal antibodies were incubated with hsv-1-infected cells in a competitive assay to block c3b binding. each of 12 different monoclonals, which recognize the four major antigenic sites of gc, completely inhibited c3b binding. with this approach, no one antigenic ...19863021981
construction and properties of a cell line constitutively expressing the herpes simplex virus glycoprotein b dependent on functional alpha 4 protein synthesis.we report the construction of a cell line constitutively expressing the glycoprotein b (gb) of herpes simplex virus (hsv) 1. the cell line was constructed in two steps. in the first, a baby hamster kidney cell line was transfected with the dna of a plasmid containing the neomycin phosphotransferase gene that confers resistance to the antibiotic g418 and the gene specifying a temperature-sensitive (ts-) alpha 4 protein of hsv-1, the major viral regulatory protein. a clonal cell line, alpha 4/c113 ...19863022001
sequences homologous to two separate transforming regions of herpes simplex virus dna are linked in two human genital tumors.ten human genital invasive squamous cell carcinomas and five human premalignant tissues were analyzed for the presence of selected sets of herpes simplex virus 2 (hsv-2) dna sequences. two vulvar tumors and one vulvar dysplastic tissue were found to contain dna sequences homologous to the bglii o fragment (coordinates 0.38-0.42) and the bglii n fragment (coordinates 0.58-0.63) of hsv-2 dna. these two fragments overlap the subsets of hsv-1 and hsv-2 dna sequences (respectively) shown previously t ...19863022470
efficacy of 2'-nor-cyclicgmp in treatment of experimental herpes virus infections.9-[(2-hydroxy-1,3,2-dioxaphosphorinan-5-yl)oxymethyl]guanine p-oxide (2'-nor-cgmp), the cyclic phosphate of 2'-nor-deoxyguanosine (2'-ndg) was synthesized by phosphorylation of 2'-ndg and evaluated for antiherpetic activity in cell cultures and in animal protection studies. 2'-nor-cgmp was effective in cell culture against both thymidine kinase deficient and wild-type herpes simplex virus type 1 strains and also against herpes simplex virus type 2. the anti-herpes activity of 2'-nor-cgmp against ...19863022643
autonomic nervous system involvement in experimental genital infection by herpes simplex virus type 2.peroxidase-antiperoxidase technique and histology were employed to elucidate the peripheral routes involved in hsv-2 progression from vagina towards the central nervous system in mice. 12 week-old female balb/c mice were intravaginally infected with 5 x 10(5)ld50 of hsv-2. sixty per cent of animals developed vulvovaginitis, perigenital alopecia and hind-limb paresia. death occurred at 9-11 days post-infection. colon dilatation and urinary bladder distention were observed in all cases. complete t ...19863022683
type specificity of complement-fixing antibody against herpes simplex virus type 2 ag-4 early antigen in patients with asymptomatic infection.we evaluated the type specificity of complement-fixing (cf) antibody against the ag-4 early antigen of herpes simplex virus (hsv) type 2 (hsv-2) by comparing a commercial ag-4 cf kit (simplex-2; gene link australia, inc., princeton, n.j.) with quantal microneutralization (mn) and absorption-western blotting in testing sera from patients with and without a history of genital herpes. sera characterized as hsv type 1 (hsv-1) or hsv-2 positive or negative by mn were selected and tested by cf, and th ...19863023439
herpes simplex virus type 2 mutagenesis: characterization of mutants induced at the hprt locus of nonpermissive xc a previous report, herpes simplex virus type 2 (hsv-2) was shown to increase the frequency of mutation at the hypoxanthine phosphoribosyltransferase (hprt) locus of nonpermissive rat xc cells (l. pilon, a. royal, and y. langelier, j. gen. virol. 66:259-265, 1985). a series of 17 independent mutants were isolated after viral infection together with 12 spontaneous noninfected mutants to characterize the nature of the mutations induced by the virus at the molecular level. the dna of the mutants ...19863023954
viral antibodies in serum and cryoprecipitate of patients with essential mixed and secondary cryoglobulinemia. preliminary results.antibodies to measles virus, cmv, hsv1, hsv2 and ebv have been tested in 9 patients with essential mixed cryoglobulinemia (emc) and 24 patients with cryoglobulinemia associated with lymphoproliferative, autoimmune or hepatic diseases (secondary cryoglobulinemia, sc). the assays were performed in serum, cryoprecipitate and supernatant by using immunoenzymatic methods. the highest prevalence was observed in the supernatant of both emc and sc patients. antibodies to measles virus show a serum/cryop ...19863024297
the nucleotide sequence of the gb glycoprotein gene of hsv-2 and comparison with the corresponding gene of hsv-1.the nucleotide sequence of the gb glycoprotein gene of hsv-2 has been determined and compared with the homologous gene of hsv-1. the two genes are specified by the same total number of codons (904); eight additional codons of the hsv-1 gene are found within the signal sequence, and eight additional codons of the hsv-2 gene are found at three different sites in the gene. the signal cleavage, membrane-spanning, and eight potential n-linked oligosaccharide sites, as well as 5'- and 3'-regulatory si ...19863024391
mode of action, toxicity, pharmacokinetics, and efficacy of some new antiherpesvirus guanosine analogs related to buciclovir.9-[4-hydroxy-3-(hydroxymethyl)butyl]guanine (3hm-hbg), (rs)-9-[4-hydroxy-2-(hydroxymethyl)butyl]guanine ([+/-]2hm-hbg), and cis-9-(4-hydroxy-2-butenyl)guanine (2en-hbg), new acyclic guanosine analogs structurally related to buciclovir (bcv [(r)-9-(3,4-dihydroxybutyl)guanine]), were evaluated in parallel with buciclovir as anti-herpes simplex virus (hsv) agents. in cell cultures, replication of different strains of hsv type 1 (hsv-1) and hsv-2 was inhibited at nontoxic drug concentrations. the co ...19863024562
characterization of acyclovir-resistant and -sensitive herpes simplex viruses isolated from a patient with an acquired immune deficiency.several different genital and non-genital hsv isolates were obtained from a patient with an acquired immune deficiency of unknown aetiology. the patient was initially treated with topical acyclovir (acv) and later with topical and intravenous acv. in spite of treatment with antiviral drugs the patient continued to shed virus and to have extensive genital ulcerations. restriction endonuclease (re) analyses of the viral dna revealed that all the isolates had characteristic hsv-2 patterns and that ...19863025153
anti-herpes virus combinations in relation to drug resistance.two broad categories of combinations have been subject to experimental investigation against hsv-1 and hsv-2; those consisting of an active antiviral drug and an inhibitor of its catabolism and those which contain two active antiviral agents. the latter seek to exploit biochemical mechanisms likely to lead to a synergistic or complementary interaction. examples of both types of combination for the suppression of resistance development and for the treatment of infection caused by resistant strain ...19863025155
the antiviral activity against herpes simplex virus of the triterpenoid compounds carbenoxolone sodium and cicloxolone sodium.dose-response experiments show that the presence of 300 microm cicloxolone sodium (ccx) or 500 microm carbenoxolone sodium (cbx) during the hsv replication cycle reduced the infectious virus yield by 10,000- to 100,000-fold: ccx is the more potent anti-herpes agent. hsv-2 replication was consistently more severely restricted by either drug than was that of hsv-1. the ed50 values obtained for either drug against hsv-1 or hsv-2 correlate well with data from dose-response curves. ccx, and to a less ...19863025156
antiviral activities of human monoclonal antibodies to herpes simplex virus.hybridomas producing human monoclonal antibodies (mabs) against herpes simplex virus (hsv) were established by fusing human tonsillar lymphocytes with mouse myeloma cells. three hybridomas have been stably producing mabs for more than 16 months. all three mabs--h1, h2, and h3--were of the igg1 isotype and recognized the gb glycoprotein of hsv types 1 and 2 (hsv-1 and hsv-2). mabs h2 and h3 not only bound to the surface membrane of hsv-infected cells but also neutralized both hsv-1 and hsv-2, whe ...19873025308
[immunohistological study on malignant and premalignant lesions of the uterine cervix associated with herpes simplex virus].herpes simplex virus 2 (hsv-2) has become the object of public attention as an etiological cause of cervical cancer. uneven distribution of hsv antigen in tissue was examined dy dyeing tissue materials of cervical intraepithelial neoplasia by the method called peroxidase-antiperoxidase using hsv-2 antibody. the positive rate in the control group was 10.3%, while it was 10.7% in the mild dysplasia group, 17.5% in the moderate dysplasia group, 25.5% in the severe dysplasia group, 31.3% in the carc ...19863025314
neonatal herpes simplex virus infection occurring in second twin of an asymptomatic mother. failure of a modern protocol.a case of neonatal herpes that occurred after vaginal delivery in the absence of genital lesions is presented. the mother had a history of drug addiction and genital herpes. asymptomatic shedding of herpes simplex virus type 2 from the cervix was noted in the second trimester of pregnancy. despite being followed with a protocol of close surveillance and serial culturing of the genital tract, the patient gave birth to twins, one of whom developed herpes simplex virus type 2 in the postpartum peri ...19873025467
isolation and characterization of expressible cdna clones encoding the m1 and m2 subunits of mouse ribonucleotide reductase.mammalian ribonucleotide reductase consists of two nonidentical subunits, proteins m1 and m2, which are differentially regulated during the cell cycle. we have isolated expressible cdna clones of both subunits from an okayama-berg cdna library made with mrna from hydroxyurea-resistant, m2 protein-overproducing mouse ta3 cells. expression of m2 protein could be demonstrated by electron paramagnetic resonance spectroscopy after transfection of cos-7 monkey cells with the plasmid. electrophoresis a ...19863025593
efficacy of vaccination with skinner vaccine towards the prevention of herpes simplex virus induced cervical carcinoma in an experimental mouse model.the incidence of cervical carcinoma, which had been induced by vaginal application of inactivated herpes simplex virus type 2 (hsv-2) with 20% croton oil, was significantly reduced in mice prevaccinated with the skinner herpes vaccine. there was evidence of an immunological response in both vaccinated and unvaccinated mice.19863026107
photodynamic inactivation of influenza and herpes viruses by hematoporphyrin.hematoporphyrin (hp), at concentrations as low as 0.5 microgram/ml, was found to inhibit the in vitro replication of influenza a and herpes simplex viruses, but not of several other viruses. the effect required exposure of the viruses or cells to visible light and was demonstrable when hp was administered shortly before virus inoculation or during the infection. in studies on the mechanism of action of hp, we found that in the presence of light, hp caused decomposition of gmp but not of various ...19873026246
evidence that neomycin inhibits hsv 1 infection of bhk cells.the effect of neomycin on the herpes simplex virus (hsv) type 1 and 2 infection of baby hamster kidney cells was studied. neomycin concentrations of 3 mm or more caused a more than 90% inhibition of hsv 1 proliferation, while it had no effect on hsv 2 proliferation, measured as plaque-forming units. furthermore, neomycin must be present at the time of infection in order to exert full effect, addition 1 hour postinfection was comparable to untreated cells. this indicates that neomycin may specifi ...19863026374
induction of cellular functions in spontaneously immortalized rat-2 cells transfected with cloned herpes simplex virus type 2 (hsv-2) dna.experiments were done to determine if cloned transforming sequences from herpes simplex virus type 2 (hsv-2) dna confer upon transfected rat-2 cells the capacity to be stimulated in phospholipase and cyclooxygenase activities following 12-o-tetradecanoylphorbol-13-acetate (tpa) treatment. tumor-derived rat-2 cells transformed with sub-fragments (bamhi-e, hindiii/hpai-ed, or psti-c) overlapping the right-hand end of the bglii-c transforming region of hsv-2 dna were stimulated by tpa in both phosp ...19873026679
transmission of infection with herpes simplex virus by renal transplantation.disseminated infection with herpes simplex virus type 2 was identified in two patients 20 days after they had received kidney transplants from the same organ donor. neither patient had neutralizing antibody to herpes simplex virus before transplantation, and both had herpes simplex virus isolated from surveillance cultures of urine before the onset of clinical symptoms. a clear focus of primary infection was not found in either patient. analysis of the patients' isolates by dna restriction endon ...19873027191
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