Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| metabolism of the carbocyclic analogue of (e)-5-(2-iodovinyl)-2'-deoxyuridine in herpes simplex virus-infected cells. incorporation of c-ivdu into dna. | the carbocyclic analogues of (e)-5-(2-bromovinyl)-2'-deoxyuridine (bvdu) and (e)-5-(2-iodovinyl)-2'-deoxyuridine (ivdu), in which the sugar moiety is replaced by a cyclopentane ring and which have been designated as c-bvdu and c-ivdu, respectively, are, like their parent compounds bvdu and ivdu, potent and selective inhibitors of herpes simplex virus type 1 (hsv-1) and, to a lesser extent, herpes simplex virus type 2 (hsv-2) replication. we have now synthesized the radiolabeled c-ivdu analogue, ... | 1985 | 2993282 |
| the therapeutic efficacy of a xanthate compound on herpes simplex virus in skin lesions of mice and guinea-pigs. | xanthates have recently been shown to inhibit the replication of both dna and rna viruses in vitro. the antiviral activity was exerted only under acidic ph conditions. curative effects in vivo on herpes simplex virus (hsv)-induced skin lesions were only observed when the xanthate compound was administered in the form of an ointment containing acidic buffer (sodium phosphate ph 5.0). advanced hsv-2-induced skin lesions in mice were healed by topical treatment with the xanthate compound. hsv-1-ind ... | 1985 | 2993486 |
| identification of cross-reacting glycoproteins of four herpesviruses by western blotting. | monospecific rabbit antisera against purified herpes simplex virus type 1 (hsv-1) gb, gc or gd antigens or polyvalent rabbit antiserum against equine herpesvirus type 1 (ehv-1)-infected cells was used in western blotting to identify antigenically related proteins in cells infected with hsv-1, hsv-2, bovine mammillitis virus or ehv-1. monomeric and oligomeric polypeptides related to hsv-1 gb were found in cells infected with each of the four herpesviruses. the gc antiserum was specific for hsv-1 ... | 1985 | 2993488 |
| serum antibodies to the major hsv-2-specified dna-binding protein in patients with an acute hsv infection or cervical neoplasia. | the major hsv-2-specified dna-binding protein (icsp 11/12) was purified from hsv-2-infected cells. elisa and immunoblotting techniques were used to study its antigenicity in hsv-infected patients and patients with cervical neoplasia and control women. patients with an acute hsv-2 infection had clearcut antibody responses to the purified icsp 11/12 preparation. determination of the icsp 11/12 antibodies by elisa revealed considerably higher serum antibody levels in patients with cervical carcinom ... | 1985 | 2993495 |
| lack of oral hsv-2 in a college student population. | a population of individuals with a high incidence of genital herpes simplex virus type 1 (hsv-1), due most likely to oro-genital contact, was examined to determine the incidence of oral herpes simplex virus type 2 (hsv-2) infection. herpes simplex virus was isolated from the oral cavity of 43 college students whose symptoms ranged from singular lesions of the lips with minimal discomfort to severe oral disease with systemic involvement resulting in lymphadenopathy, chills, sweat, myalgia, and fe ... | 1985 | 2993498 |
| synthesis and antiherpes simplex virus activity of 9-[(1,3-dihydroxy-2-propylthio)methyl]guanine. | the synthesis of the thio analogue (thio-dhpg, 2) of 9-[(1,3-dihydroxy-2-propoxy)methyl]guanine (dhpg, 1) is described. the synthesis of 2 proceeded via the condensation of acetoxymethyl sulfide 9 with diacetylguanine 10 to give the protected nucleoside analogue 11. although catalytic hydrogenolysis failed, the benzyl ether functionalities of 11 were successfully cleaved by an acetolysis reaction to furnish 14. ammonolysis of 14 gave 2, which was also transformed to sulfoxide 15 and sulfone 16. ... | 1985 | 2993615 |
| application of antibody to synthetic peptides for characterization of the intact and truncated alpha 22 protein specified by herpes simplex virus 1 and the r325 alpha 22- deletion mutant. | the alpha 22 protein is one of five proteins synthesized immediately after infection of permissive cells with herpes simplex virus 1 and 2 (hsv-1 and hsv-2). on the basis of the reported nucleotide sequence of the hsv-1 gene, we synthesized two peptides containing the predicted amino acids 12 through 23 (12 residues) and 21 through 36 (16 residues) in two hydrophilic domains near the n terminus of the protein. rabbit antisera made against these peptides were then used to characterize the alpha 2 ... | 1985 | 2993651 |
| virion component of herpes simplex virus type 1 kos interferes with early shutoff of host protein synthesis induced by herpes simplex virus type 2 186. | herpes simplex virus (hsv) strains hsv type 1 (hsv-1) kos and hsv-2 186 are representative of delayed and early shutoff strains, respectively, with regard to their ability to inhibit protein synthesis in friend erythroleukemia cells. when these cells were simultaneously infected with hsv-1 kos and hsv-2 186, hsv-1 kos interfered with the rapid suppression of globin synthesis induced by hsv-2 186. the observed interference was competitive and not due to exclusion of hsv-2 by hsv-1 at the level of ... | 1985 | 2993660 |
| cytomegalovirus and herpes simplex virus ascending myelitis in a patient with acquired immune deficiency syndrome. | progressive ascending myelitis was the presenting feature of the acquired immune deficiency syndrome (aids) in a homosexual man who also had kaposi's sarcoma, pneumocystis pneumonia, and disseminated cytomegalovirus (cmv) infection. neuropathological studies showed profuse cytomegalic cells throughout the brain and spinal cord, but no inflammatory response. at postmortem examination, cmv and herpes simplex virus, type 2 (hsv-2), were recovered from multiple sites throughout the central nervous s ... | 1985 | 2994553 |
| immunoblotting and enzyme-linked immunosorbent assay analysis of serological responses in patients infected with herpes simplex virus types 1 and 2. | serological responses to soluble membrane and cytoplasmic antigens specified by herpes simplex type 1 (hsv1) and 2 (hsv2) were studied by immunoblotting and by enzyme-linked immunosorbent assay (elisa). in the immunoblotting test, polypeptides migrating like the hsv1-specified glycoprotein c showed type-specific reactivity but could not always be detected. the immunoblotting and elisa results were in agreement when antibody responses to hsv1- and hsv2-specified antigens were compared, and they a ... | 1985 | 2995271 |
| differences in humoral immunogenicity between herpes simplex virus types 1 and 2. | infection of nmri mice with increasing doses of six different strains of herpes simplex virus type 1 (hsv-1) induced increasing levels of neutralizing antibodies. in contrast, three strains of hsv-2, irrespective of the dose, induced only marginal antibody responses. only strain hg 52 (hsv-2) at high doses of infection stimulated antibody formation. the virus content of some organs in 6- to 8-week-old mice appeared to be lower after hsv-2 than after hsv-1 infection. application of immune-modulat ... | 1985 | 2995556 |
| inhibition of herpes simplex virus replication in vitro by human cytotoxic t cell clones and natural killer cell clones. | the abilities of human cytotoxic t cell (ctl) clones and natural killer (nk) cell clones to inhibit the replication of herpes simplex virus (hsv) in vitro were shown. the specificities of clones inhibiting hsv replication were the same as those of cytotoxicity in hsv type specificity and hla restriction, i.e. hsv type 1 (hsv-1) and hsv type 2 (hsv-2) common ctl clones inhibited the replication of both hsv-1 and hsv-2 in autologous cells, but not in allogeneic cells. hsv-1-specific ctl clones inh ... | 1985 | 2995557 |
| acute and latent herpes simplex virus neurological disease in mice immunized with purified virus-specific glycoproteins gb or gd. | groups of 5-week-old balb/c mice were immunized intraperitoneally with approximately 10 micrograms of purified alum-precipitated glycoprotein gb or gd of either herpes simplex virus type 1 (hsv-1) or type 2 (hsv-2) origin. control mice received injections of alum-precipitated 1% bovine serum albumin (bsa). following a second immunization 4 weeks later, seroconversion was confirmed by demonstrating the presence of glycoprotein-specific antibody by immune precipitation. all animals were challenged ... | 1985 | 2995573 |
| (+/-)-2-amino-3,4-dihydro-7-[2,3-dihydroxy-4-(hydroxymethyl)-1- cyclopentyl]-7h-pyrrolo[2,3-d]pyrimidin-4-ones: new carbocyclic analogues of 7-deazaguanosine with antiviral activity. | 5-allyl-2-amino-4,6-dihydroxypyrimidine (3) was chlorinated and ozonized to yield (2-amino-4,6-dichloro-pyrimidin-5-yl)acetaldehyde (5). acetalization of 5 with ethanol afforded a new pyrimidine intermediate 6 which can lead to 2-amino-3,4-dihydro-7-alkyl-7h-pyrrolo[2,3-d]pyrimidin-4-ones and therefore to carbocyclic analogues of 7-deazaguanosine. the 7-substituent was a cyclopentyl analogue of the arabinofuranosyl moiety in 10a, lyxofuranosyl moiety in 10b, and ribofuranosyl moiety in 10c. comp ... | 1985 | 2995667 |
| effect of dimethyl sulfoxide on interaction of human cytomegalovirus with host cell: conversion of a nonproductive state of cell to a productive state for virus replication. | the effect of dimethyl sulfoxide (dmso) on the interaction of human cytomegalovirus (hcmv) with host cell was studied. confluent state of a human rhabdomyosarcoma cell line (a204) showed a much lower susceptibility to hcmv infection when compared to that in subconfluent actively growing cell cultures. treatment of confluent cultures with dmso, however, converted many nonproductive cells in these cultures to a productive state for virus replication. infectious center assay revealed that approxima ... | 1985 | 2996216 |
| immunoperoxidase staining for the detection of herpes simplex virus antigen in cervicovaginal smears. | destained cervicovaginal smears from eight patients with herpes simplex virus (hsv) infections were stained by means of the peroxidase-antiperoxidase (pap) technique to demonstrate the presence of the hsv type 2 (hsv-2) antigen. positive results were obtained in six of the eight cases, with intense staining for the hsv-2-specific antigen throughout the cytoplasm and nuclei of cells having a ground-glass nuclear appearance as well as in multinucleated giant cells. virus isolation was successfully ... | 1985 | 2996270 |
| herpes simplex viral infection in human neonates: an immunohistochemical and electron microscopic study. | specimens obtained at autopsy from six neonates with herpes simplex virus (hsv) infections were examined microscopically, electron microscopically, and immunohistochemically. coagulative necrosis with inclusions was found in the livers and adrenal glands in all cases, as well as in various other organs, including the spleen, bone marrow, lungs, esophagus, tongue, and thymus, in some cases. distinct hemorrhagic diathesis was found in three cases. no characteristic clinical findings, such as skin ... | 1985 | 2997017 |
| the sources of thymidine nucleotides for virus dna synthesis in herpes simplex virus type 2-infected cells. | the thymidine nucleotide sources present during herpes simplex virus type 2 (hsv-2) infection were examined. it was concluded that the source of dttp in hsv-2-infected cells is not only derived from the ribonucleotide reductase-catalyzed de novo pathway, but also from host dna. when the de novo pathway was inhibited by the addition of hydroxyurea, an inhibitor of ribonucleotide reductase, the dttp levels were maintained by a compensatory increase in dttp derived from host dna. the utilization of ... | 1985 | 2997161 |
| humoral immune response to herpes simplex virus type 2 glycoproteins in patients receiving a glycoprotein subunit vaccine. | serial serum specimens from 22 herpes simplex virus (hsv)-seronegative recipients of an hsv type 2 (hsv-2) glycoprotein subunit vaccine were analyzed by radioimmunoprecipitation and polyacrylamide gel electrophoresis for the development of antibodies to hsv-2 gb, gd, and g80, a complex of gc and ge. volunteers received 50 (n = 12) or 100 micrograms (n = 10) of vaccine at days 0, 28, and 140; sera were drawn weekly for 8 weeks and again at days 140, 147, and 365. among seronegative volunteers, an ... | 1985 | 2997470 |
| evaluation of two rapid methods for the detection of herpes simplex virus antigen in patient specimens. | an indirect immunofluorescent antibody procedure (ifa) for the detection and typing of herpes simplex virus (hsv) and an enzyme-linked immunosorbent assay (elisa) procedure were compared with conventional viral culture. specimens for culture and elisa were inoculated into serum free viral transport medium (vtm) and, for ifa, onto slides provided in the kit. tissue cultures (mrc-5 and primary rabbit kidney) were inoculated and examined daily for cytopathogenic effect (cpe). the remaining vtm was ... | 1985 | 2998264 |
| topical butylated hydroxytoluene treatment of genital herpes simplex virus infections of guinea pigs. | the effect of topical treatment with butylated hydroxytoluene (bht) was evaluated in primary and recurrent genital herpes simplex virus type 2 (hsv-2) infection of guinea pigs. in the first experiment, treatment with placebo, 5%, 10%, or 15% bht was initiated 48 h after viral inoculation and continued 4 times daily for 15 days. during primary infection no differences in maximum lesion severity or titers of virus in lesions were observed, however, lesion duration was reduced in bht-treated animal ... | 1985 | 2998276 |
| delta-9-tetrahydrocannabinol decreases host resistance to herpes simplex virus type 2 vaginal infection in the b6c3f1 mouse. | the effect of delta-9-tetrahydrocannabinol (delta-9-thc) on host resistance to herpes simplex virus type 2 (hsv-2) vaginal infection in the b6c3f1 mouse was determined. animals were given delta-9-thc or vehicle on days -1 to 2, or cyclophosphamide on day -1 or on days -1 to 2. hsv-2 was introduced intravaginally on day 0. host resistance to virus infection was assessed by comparing frequency and severity of lesions, virus shedding and mortalities. replicate groups of animals were bled on days 5, ... | 1985 | 2999306 |
| synthetic glycoprotein d-related peptides protect mice against herpes simplex virus challenge. | glycoprotein d (gd) of herpes simplex virus (hsv) protects mice from a lethal challenge by either hsv type 1 (hsv-1; oral) or hsv-2 (genital). we evaluated whether synthetic peptides representing residues 1 through 23 of gd (mature protein) can be used as a potential synthetic herpesvirus vaccine. the immunogenicity of the peptides was demonstrated by the biological reactivity of antipeptide sera in immunoprecipitation and neutralization assays. all sera which immunoprecipitated gd had neutraliz ... | 1985 | 2999419 |
| effect of herpes simplex virus types 1 and 2 on surface expression of class i major histocompatibility complex antigens on infected cells. | cytotoxic t lymphocytes (ctl) generated in c57bl/6 (h-2b) mice in response to infection with the serologically distinct herpes simplex virus type 1 (hsv-1) or type 2 (hsv-2) were cross-reactive against target cells infected with either serotype. however, hsv-2-infected cells were shown to be much less susceptible to ctl-mediated lysis, and analysis through the use of hsv-1 x hsv-2 intertypic recombinants mapped the reduced susceptibility to a region contained within 0.82 to 1.00 map units of the ... | 1985 | 2999432 |
| a modular system for the assay of transcription regulatory signals: the sequence taatgarat is required for herpes simplex virus immediate early gene activation. | a modular system for assaying the activity of transcriptional regulatory signals based on herpes simplex virus (hsv) promoter and terminator sequences linked to the bacterial chloramphenicol acetyltransferase (cat) gene has been used to study activation of hsv immediate early (ie) gene expression. insertion of the sv40 72 base pair (bp) repeat increased mrna levels by 15-fold thus demonstrating the ability of the hsv ie promoter to respond to a heterologous enhancer. a fragment containing part o ... | 1985 | 2999706 |
| expression of the herpes simplex virus type-2 major dna-binding protein (icp8) gene in cos-1 cells. | by the use of an sv40 origin of replication plasmid vector and the cos-1 cell system, expression of the gene encoding the herpes simplex virus type-2 (hsv-2) major dna binding protein (icp8) has been achieved. the hsv-2 4.5 kb bglii 0 dna fragment containing the icp8 coding region was inserted into the plasmid vector psvod containing the sv40 origin of replication. transfection of cos-1 cells by the resultant recombinant plasmid (psvod20), and subsequent multiplication of this plasmid, led to th ... | 1985 | 3000103 |
| synergistic effect of human leukocyte interferon and nonoxynol 9 against herpes simplex virus type 2. | the nonionic surfactant nonoxynol 9 (np9), in combination with human alpha interferon, synergistically reduced the titer of herpes simplex virus type 2 (hsv-2) in vitro. the degree of synergy was highest at an interferon concentration of 10(3) iu/ml and an np9 dilution of 1:1,500. we postulate that np9 inactivates extracellular hsv-2, whereas interferon inhibits hsv-2 replication at the intracellular level. | 1985 | 3000288 |
| antiviral activity of 5-ethyl-2'-deoxyuridine against herpes simplex viruses in cell culture, mice, and guinea pigs. | the susceptibility of 3 laboratory strains and 24 clinical isolates of herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) to 5-ethyl-2'-deoxyuridine was determined in plaque reduction assays in vero cells. the median effective doses were 8.6 and 7.8 microm, respectively. the drug was less potent than acyclovir and other related antiviral drugs, but it had a high therapeutic index against both hsv-1 and hsv-2. drug-resistant viruses were readily produced in cell culture. these variants were c ... | 1985 | 3000291 |
| construction of rat cell lines that contain potential morphologically transforming regions of the herpes simplex virus type 2 genome. | hybrid recombinant plasmids were constructed; they were composed of the herpes simplex virus type 2 (hsv2) thymidine kinase (tk) gene and dna sequences of hsv2 that have been reported to induce morphological and/or oncogenic transformation of rodent cells in culture. several plasmids were made in two versions, with or without the simian virus 40 enhancer sequences. these plasmids were employed to transfect contact-inhibited rat-2 tk- cells. it was demonstrated that cell lines with stably integra ... | 1985 | 3000980 |
| recurrent genital herpes simplex virus infection in guinea pigs. | after recovery from initial genital herpes simplex virus (hsv) infections, female guinea pigs developed spontaneous recurrent infections characterized by discrete erythematous or vesicular herpetic lesions on the external genital skin. hsv type 2 (hsv2) caused significantly more recurrent infections in guinea pigs than did hsv type 1 (hsv1). hsv2-infected animals demonstrated a significant decline in frequency of recurrences over time. the viral nature of the recurrent lesions was confirmed by r ... | 1985 | 3000982 |
| the cervical tumor-associated antigen (icp-10/ag-4) is encoded by the transforming region of the genome of herpes simplex virus type 2. | bglii fragment c mapping between 0.416 and 0.580 map units (mu) on the herpes simplex virus type 2 (hsv-2) genome was used for in vitro translation to identify proteins encoded on this fragment. rna homologous to the bglii c fragment directs the synthesis of three proteins with approximate molecular weights of 144,000, 52,000 and 27,000. the 27,000 dalton protein is encoded by sequences within the ecori/hindiii ae fragment (0.419-0.525 mu) that overlap the immortalizing sequences within bglii c. ... | 1985 | 3001010 |
| typing of herpes simplex virus isolates with monoclonal antibodies and by nucleic acid spot hybridization. | fifty-one clinical isolates of herpes simplex virus (hsv) were typed by an enzyme immunoassay (eia) using mouse monoclonal antibodies, by dna spot hybridization, and by restriction enzyme analysis using restriction endonuclease eco ri. extracts of vero cells infected with the isolates were used for coating microtitre plates or denatured and spotted onto nitrocellulose filters. viral antigens passively adsorbed to microtitre plates were detected by an indirect eia using mouse monoclonal antibodie ... | 1985 | 3001118 |
| detection of herpes simplex virus type 2-specific antibody with glycoprotein g. | a recently described herpes simplex virus (hsv) type 2 (hsv-2)-specific glycoprotein (gg-2) was purified on an immunoaffinity column prepared with monoclonal antibody. this purified antigen was used in an immunodot enzymatic assay on nitrocellulose paper for the detection of hsv-2 antibodies in human serum. the test was very sensitive in that hsv-2 antibodies were detected in the convalescent sera of 132 of 134 patients with recurrent genital infections in which hsv-2 had been isolated earlier. ... | 1985 | 3001136 |
| detection of herpes simplex virus type-2 antigen(s) in biopsies from carcinoma of the uterine cervix. | indirect immunofluorescence technique was employed to detect herpes simplex virus type-2 (hsv-2) antigens in tumor biopsies from 215 patients with carcinoma of the uterine cervix. a total of 169 samples (79%) revealed brilliant nuclear fluorescence. inflammatory cells infiltrating the tumor mass were positive to 60 of the 215 patients (28%). samples showed no significant variation in the degree of fluorescence or proportion of cells binding hsv antibody with advancement in the clinical stage of ... | 1985 | 2431113 |
| the effect of food dye and other environmental substances on the host defense reaction in mice in relation to virus infection. | environmental substances were examined for their effect on interferon induction and delayed type hypersensitivity (dth) responses in mice. amaranth, safrole, phenacetin and nicotine suppressed the dth response, and suppressed the serum interferon titers induced by virus infection. however, they did not affect the interferon titers which were induced by tilorone, a chemical inducer. the peak of interferon titer was 12 hours after infection with herpes simplex virus type 2 (hsv-2). therefore, amar ... | 1986 | 2432279 |
| enhancement of host resistance against virus infections by mtp-pe, a synthetic lipophilic muramyl peptide--i. increased survival in mice and guinea pigs after single drug administration prior to infection, and the effect of mtp-pe on interferon levels in sera and lungs. | muramyl tripeptide-phosphatidylethanolamine (mtp-pe, cgp 19835) displays prophylactic antiviral activity in mice infected with influenza viruses a and b, parainfluenza 1 virus or herpes simplex type 1 viruses (hsv/1) and in guinea pigs infected with herpes simplex type 2 viruses (hsv/2). mtp-pe is effective when given in a single intranasal dose as early as 1-4 weeks before infection. in the case of hsv/2 infections, prophylactic effectiveness can be demonstrated after a single topical applicati ... | 1986 | 2433236 |
| immunity to herpes simplex virus type 2: viral antigen-presenting capacity of epidermal cells and its impairment by ultraviolet irradiation. | ia+ epidermal cells (ec) had accessory cell function for herpes simplex virus type 2 (hsv-2)-induced t cell proliferation of immune lymph node cells (lnc). the ec-mediated virus-induced proliferative response of immune lnc was inhibited by ultraviolet b (uvb) irradiation. when ec were pulsed with viral antigen before uvb irradiation, the response was partially restored by addition of epidermal cell-derived thymocyte-activating factor (etaf). although normal ec secreted prostaglandin e2, the leve ... | 1986 | 3001186 |
| induction of recurrent genital herpes simplex virus type 2 infection by ultraviolet light. | 1986 | 2870285 | |
| studies on interactions of dtk-hsv mutants with neurons in vitro. | thymidine kinase negative (dtk-) mutants of herpes simplex virus type 1 (hsv-1) multiplied well in rat brain glioma cells. a proportion (less than 1%) of glioma cells survived the infection with hsv and were designated "survivor" glioma cells. survivor cells of dtk- mutant virus infection ceased to produce infectious virus after two passages and were highly resistant to both hsv-1 and hsv-2 but not to vesicular stomatitis virus (vsv). flow cytometric studies indicated morphological differences b ... | 1986 | 2871727 |
| early intrathecal production of specific igm and igg antibodies and alpha-interferon in herpes simplex virus encephalitis. | a complex approach was used in order to establish non-invasively the aetiology in three cases of encephalitis presumptively caused by herpes simplex virus (hsv). as indicative of brain hsv infection was considered the lowered serum to cerebrospinal fluid specific antibody ratio, which also assessed the humoral immune response within the cns. for this purpose, during ongoing brain tissue infection, the early intrathecal (ith) production of igg and igm antibodies was analysed by a differential enz ... | 1986 | 2873733 |
| induction of chromosomal aberrations in human cells by a temperature-sensitive mutant of herpes simplex virus type 2 and its revertants. | the induction of chromosomal aberrations by a temperature-sensitive (ts) mutant of herpes simplex virus type 2 (hsv-2) strain hg52 (ts 13), its revertants 4-8 and 5-8 and by etalon strains hsv-1 17 syn+ and hsv-2 hg52 was studied in human fibroblast and lymphocyte cultures. the effect on chromosomes of the revertants was tested at permissive (31 degrees c) and non-permissive (38 degrees c) temperatures. at 38 degrees c the revertants could not induce dnase activity. the present results contribut ... | 1986 | 2874732 |
| vaccine potential of a live avirulent herpes simplex virus. | we previously described a herpes simplex intertypic recombinant that is completely and specifically non-neurovirulent following intracranial inoculation. in this report the vaccine potential of this mutant (re6) is analyzed. it is shown that re6 replicates to wild type levels at the peripheral site of inoculation (the mouse footpad) but, unlike its virulent parental strains, does not replicate to detectable levels in lumbosacral ganglia. a single vaccination with re6 is sufficient to completely ... | 1986 | 2854601 |
| transcriptional induction of cellular gene expression during lytic infection with herpes simplex virus. | herpes simplex virus type 2 causes a severe repression of host cell biosynthesis at a number of levels. we show that despite this, non-viral cdna clones derived from cellular rna species which accumulate to high levels after infection can be isolated using differential screening techniques. by using nuclear run-off assays, we have shown that this rna accumulation is mediated by transcriptional induction of the corresponding cellular genes. | 1986 | 2883998 |
| acyclovir treatment of disseminated herpes simplex virus type 2 infection in weanling mice: alteration of mortality and pathogenesis. | intranasal inoculation of weanling mice with herpes simplex virus type 2 (hsv-2) provides an experimental infection that closely resembles disseminated and central nervous system hsv infections of human neonates. intraperitoneal treatment with acyclovir (acv) successfully reduced mortality even when therapy was begun as late as 2 days and oral therapy as late as 4 days after viral challenge. treatment with acv beginning on day 1 completely inhibited hsv-2 replication in lung, spleen, kidney, olf ... | 1986 | 2427021 |
| antiviral effect of two aryl-oligopeptides, fr41565 and fr48217. | macrophages from mice pretreated with two chemically synthesized immunostimulating aryl-oligopeptides, fr41565 and fr48217, inhibited the multiplication of herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) in monkey vero cells, and that of vesicular stomatitis virus (vsv) in murine l929 cells. in addition, the aryl-oligopeptides protected mice against a lethal hsv-1 infection. in particular, when treated with fr48217 at 6 mg/kg, all mice survived, whereas all control mice died from the hsv- ... | 1986 | 2427022 |
| localization of discontinuous epitopes of herpes simplex virus glycoprotein d: use of a nondenaturing ("native" gel) system of polyacrylamide gel electrophoresis coupled with western blotting. | previously, a panel of monoclonal antibodies (mcab) was used to define specific epitopes of herpes simplex virus glycoprotein d (gd) (r. j. eisenberg et al., j. virol. 53:634-644, 1985). three groups of antibodies recognized continuous epitopes; group vii reacted with residues 11 to 19 of the mature protein (residues 36 to 44 of the predicted sequence), group ii reacted with residues 272 to 279, and group v reacted with residues 340 to 356. four additional antibody groups recognized discontinuou ... | 1986 | 2427745 |
| structural features of ribonucleotide reductase. | herpes simplex virus type 1 (hsv-1) encodes a ribonucleotide reductase which comprises two polypeptides with sizes of 136,000 (rr1) and 38,000 mol. wt. (rr2). we have determined the entire dna sequence specifying hsv-1 rr1 and have identified two adjacent open reading frames in varicella-zoster virus (vzv) which have homology to hsv rr1 and rr2; the predicted sizes for the vzv rr1 and rr2 polypeptides are 87,000 and 35,000 mol. wt. respectively. amino acid comparisons with rr1 and rr2 polypeptid ... | 1986 | 2835765 |
| herpes simplex virus specifies two subunits of ribonucleotide reductase encoded by 3'-coterminal transcripts. | we have previously described a transcription unit located between map coordinates 0.558 and 0.595 on the herpes simplex virus type 2 strain 333 genome which encodes two mrnas of 5.0 and 1.2 kilobases that share a common 3' terminus, and we have determined the nucleotide sequence of a 38,000-dalton protein specified by the smaller rna (d. a. galloway and m. a. swain, j. virol. 49:724-730, 1984). the entire nucleotide sequence of the 140,000-dalton protein specified by a 3,432-base-pair open readi ... | 1986 | 2419588 |
| human antibody response to herpes simplex virus-specific polypeptides after primary and recurrent infection. | human antibody responses to specific polypeptides of herpes simplex virus types 1 and 2 (hsv-1 and hsv-2, respectively) were assessed in serial serum specimens from 18 infected patients by immunoblot technology. nine patients had hsv-1 infections (six genital and three oral) and nine had hsv-2 genital infections. antibodies to homologous and heterologous hsv antigens were studied and correlated with total microneutralization and enzyme-linked immunosorbent assay antibodies as well as correlated ... | 1986 | 2422204 |
| human cytotoxic t cell clones directed against herpes simplex virus-infected cells. iv. recognition and activation by cloned glycoproteins gb and gd. | results of studies in mice and clinical observations in man indicate that t cell-mediated immunity is important in resistance to herpes simplex virus (hsv) infections. this study was undertaken to elucidate the viral antigen specificity of human hsv-immune t cells. purified hsv-1 glycoproteins gb-1 and gd-1, cloned and expressed in mammalian cells, were found to stimulate proliferation of, and interleukin 2 (il 2) production by, peripheral blood lymphocytes (pbl) of hsv seropositive individuals, ... | 1986 | 2423598 |
| an analysis of the biological properties of monoclonal antibodies against glycoprotein d of herpes simplex virus and identification of amino acid substitutions that confer resistance to neutralization. | four monoclonal antibodies to glycoprotein d (gd) of herpes simplex virus (hsv) types 1 and 2 neutralized virus in the presence of complement but exhibited diverse activities in its absence. amino acid substitutions that conferred resistance to neutralization by each antibody were identified by deriving the nucleotide sequence of the gd gene from resistant mutants. each antibody selected a substitution from different parts of the molecule and mutants resistant to a single antibody always arose f ... | 1986 | 2423636 |
| metabolism and mode of action of (r)-9-(3,4-dihydroxybutyl)guanine in herpes simplex virus-infected vero cells. | the metabolism and mode of action of the anti-herpes compound buciclovir [r)-9-(3,4-dihydroxybutyl)-guanine, bcv) has been studied in herpes simplex virus-infected and uninfected vero cells. in uninfected cells, a low and constant concentration of intracellular bcv was found, while in herpes simplex virus-infected cells, an increasing concentration of bcv phosphates was found due to metabolic trapping. the major phosphorylation product was bcv triphosphate (bcvtp) which was 92% of the total amou ... | 1986 | 2418021 |
| induction of neutralizing antibody against varicella-zoster virus (vzv) by vzv gp3 and cross-reactivity between vzv gp3 and herpes simplex viruses gb. | glycoprotein gp3 (64k) is one of the major proteins specified by varicella-zoster virus (vzv). this glycoprotein was purified on an immunoadsorbent consisting of monoclonal antibody (clone 8) against gp3 linked to protein a-sepharose. rabbits were then immunized with the purified antigen to obtain monospecific antisera against gp3. the monospecific antisera and monoclonal antibody immunoprecipitated polypeptides with the same molecular weights of approximately 64,000 (64k), 106k, and 116k from a ... | 1986 | 2418583 |
| synthesis of dihydrofolate reductase and metabolism of related rna in a methotrexate resistant human cell line infected with herpes simplex virus type 2. | using a human cell line with amplified gene copy for dihydrofolate reductase (dhfr) and permissive for herpes simplex virus type 2 (hsv-2), the effect of hsv-2 on dhfr synthesis and on the steady-state level of total cellular and nuclear dhfr rna was examined. there was a reduction in dhfr synthesis accompanied by a proportional decrease in the levels of dhfr messenger rna (mrna) 1 hr after infection. both effects could be induced by hsv pretreated with ultraviolet light and this early virus ind ... | 1986 | 2418585 |
| antimicrobial activity of various immunomodulators: independence from normal levels of circulating monocytes and natural killer cells. | the effects of 89sr treatment on the natural host resistance of cd-1 mice and the enhancement of resistance by immunomodulators to infection with listeria monocytogenes or herpes simplex virus type 2 (hsv-2) were determined. in the cd-1 mouse, single-dose treatment with 89sr caused a profound decrease in the number of circulating monocytes (mo), lymphocytes, and polymorphonuclear leukocytes (pmn) within 1 week. there was also marked functional impairment of the mo inflammatory response, as well ... | 1986 | 2416693 |
| delta 9-tetrahydrocannabinol decreases alpha/beta interferon response to herpes simplex virus type 2 in the b6c3f1 mouse. | this study was undertaken to determine the effect of delta 9-tetrahydrocannabinol (delta 9-thc) on polyinosinic:polycytidylic acid [poly(i):poly(c)]-induced, and on herpes simplex virus type 2 (hsv-2)-induced, alpha/beta interferon in the b6c3f1 mouse. animals were administered delta 9-thc, or the diluent, intraperitoneally for 4 consecutive days or at various time intervals prior to administration of the interferon inducer. poly(i):poly(c) or hsv-2 was injected intravenously on day 4. animals r ... | 1986 | 3003756 |
| [immunohistological study on malignant and premalignant lesions of the uterine cervix associated with herpes simplex virus]. | herpes simplex virus 2 (hsv-2) has become the object of public attention as an etiological cause of cervical cancer. uneven distribution of hsv antigen in tissue was examined dy dyeing tissue materials of cervical intraepithelial neoplasia by the method called peroxidase-antiperoxidase using hsv-2 antibody. the positive rate in the control group was 10.3%, while it was 10.7% in the mild dysplasia group, 17.5% in the moderate dysplasia group, 25.5% in the severe dysplasia group, 31.3% in the carc ... | 1986 | 3025314 |
| purulent penile ulcers of patients in singapore. | in 80 patients with painful purulent penile ulcers who attended the outpatient service of middle road hospital in singapore, haemophilus ducreyi was isolated from 18 (22%), herpes simplex virus type 2 from nine (11%), and neisseria gonorrhoeae from eight (10%). primary pathogens were not isolated from 45 (57%) men. painful purulent penile ulcers were more common in uncircumcised men, and patients had often acquired the disease after sexual intercourse with prostitutes in singapore. | 1986 | 3015775 |
| prospective study on the relationship between cervical neoplasia and herpes simplex type 2 virus. iii. presence of herpes simplex type-2 antibody in sera of subjects who developed cervical neoplasia later in the study. | paired sera taken from 63 subjects who developed cervical neoplasia in the course of a prospective study on this disease were examined for the presence of herpes simplex virus type 2 (hsv-2) antibody. the first serum was taken at enrollment into the study, while the second was obtained after development of the disease, i.e. 2-4 years later. simultaneously, paired sera from a group of control subjects, who remained free of any cytological and colposcopical abnormalities throughout the study, were ... | 1986 | 3015805 |
| antibody response, recurrence patterns and subsequent herpes simplex virus type 2 (hsv-2) re-infection following initial hsv-2 infection of guinea-pigs: effects of acyclovir. | the production of antibody to specific herpes simplex virus type 2 (hsv-2) polypeptides, the recurrence patterns and the susceptibility to re-infection were studied in the guinea-pig model of genital hsv-2 infection. further, we defined the effects of acyclovir (acv) therapy on these parameters of infection. treatment with acv reduced the clinical severity of the initial disease but did not affect vaginal viral shedding. production of neutralizing antibody as well as antibody to the nucleocapsid ... | 1986 | 3016155 |
| asymptomatic virus shedding in men with genital herpes infection. | 13 men with a history of recurrent genital herpes simplex virus type 2 (hsv-2) infection were followed daily for 4 weeks with samples taken from the urethra for virus isolation. asymptomatic virus shedding occurred in 5 men who had 1 single positive isolation each. four of these urethral isolates were typed as hsv-1 and 1 as hsv-2. | 1986 | 3016883 |
| a rapid detection of herpes simplex virus by elisa in asymptomatic pregnant women and neonates. | herpes simplex virus (hsv) was detected by the enzyme-linked immunosorbent assay (elisa). the assay system employed rabbit anti-hsv-2-coated microplates to detect hsv in clinical specimens and the same reagent labelled with peroxidase as a conjugate. the hsv type 2 obtained from vero cell culture and normal cell lysate (ncl) were used as positive and negative reference antigens respectively. hsv was detected in 40 (9.93%) of vaginal swabs obtained from 403 pregnant women just before the deliveri ... | 1986 | 3016912 |
| the herpes simplex virus type 2 equivalent of the herpes simplex virus type 1 us7 gene and its flanking sequences. | nucleotide sequencing studies (d. j. mcgeoch, a. dolan, s. donald, and f. rixon, 1985, j. mol. biol. 181, 1-14) have indicated that herpes simplex virus type 1 (hsv-1) has a coding sequence, referred to as us7, between the genes for the glycoproteins d and e (gd and ge). northern blot analysis and nucleotide sequencing have been carried out to show that the type 2 virus (hsv-2) has an equivalent to the us7 gene. a comparison with the hsv-1 sequence has revealed some surprising similarities and d ... | 1986 | 3016980 |
| evidence that neomycin inhibits hsv 1 infection of bhk cells. | the effect of neomycin on the herpes simplex virus (hsv) type 1 and 2 infection of baby hamster kidney cells was studied. neomycin concentrations of 3 mm or more caused a more than 90% inhibition of hsv 1 proliferation, while it had no effect on hsv 2 proliferation, measured as plaque-forming units. furthermore, neomycin must be present at the time of infection in order to exert full effect, addition 1 hour postinfection was comparable to untreated cells. this indicates that neomycin may specifi ... | 1986 | 3026374 |
| molecular cloning of herpes simplex virus type 2 dna. | restriction enzyme hindiii digestion of the whole genome of herpes simplex virus type 2 strain 186 yielded 10 dna fragments with molecular weights ranging from approximately 22 x 10(6) to 1.2 x 10(6), which were cloned into the hindiii site of bacterial plasmid pacyc 184. the cloned fragments were identified by hybridization to hsv-2 virus dna and by double digestion with restriction endonucleases. the recombinant plasmids, even if they carried dna sequences with molecular weights of more than 1 ... | 1986 | 3011764 |
| acute genital infection in guinea pigs: effect of recombinant interleukin-2 on herpes simplex virus type 2. | human recombinant interleukin-2 (ril-2) modifies infection with herpes simplex virus type 2 (hsv-2) in normal guinea pigs. animals were injected sc with ril-2 twice a day, beginning 24 hr before infection and continuing for three subsequent days. guinea pigs were assigned to five different regimens in which ril-2 was administered daily at dosages from 8 x 10(3) u/kg to 8 x 10(5) u/kg. after intravaginal inoculation with hsv-2, 83% of 24 control animals developed apparent genital herpes, and 5% h ... | 1986 | 3011918 |
| suppression and enhancement of humoral antibody formation by herpes simplex virus types 1 and 2. | intraperitoneal infection of mice and rats by herpes simplex virus type 2 (hsv-2) but not type 1 (hsv-1) resulted in suppression of antibody formation on subsequent challenge with hsv-1 or hsv-2. application of silica considerably enhanced antibody formation after primary hsv-1 infection, but only slightly after primary hsv-2 infection. suppression induced by hsv-2 was, however, reduced significantly by injection of silica 21 days later, on the day of the second injection of hsv-2. suppression c ... | 1986 | 3011967 |
| x-linkage of the early in vitro alpha/beta interferon response of mouse peritoneal macrophages to herpes simplex virus type 2. | the genetics of the early interferon response of mouse peritoneal cells to infection with herpes simplex virus type 2 (hsv-2) was studied in susceptible balb/c and more resistant c57bl/6 mice and in reciprocal crosses between these mice. wash-outs of the peritoneal cavity of normal c57bl/6 mice contained significantly more cells than wash-outs from balb/c mice. therefore, interferon induction with hsv-2 was studied under standardized conditions in vitro. peritoneal cells reacted to hsv-2 infecti ... | 1986 | 3011968 |
| the herpes simplex virus type 2 alkaline dnase activity is essential for replication and growth. | a mutant of herpes simplex virus type 2 (hsv-2), which is temperature-sensitive (ts) for the induction of an alkaline dnase activity, was examined at a number of different temperatures. induction of dnase activity by this mutant resembled that of wild-type (wt) virus at 31 degrees c but was greatly reduced at 38.5 degrees c and barely detectable at 39.2 degrees c. virus dna synthesis showed similar patterns, exhibiting wt levels at 31 degrees c, reduced levels at 38.5 degrees c and very little i ... | 1986 | 3011973 |
| inhibition of herpes simplex virus-induced dna polymerases and cellular dna polymerase alpha by triphosphates of acyclic guanosine analogs. | the triphosphates of the antiherpes acyclic guanosine analogs (r)- and (s)-enantiomers of 9-(3,4-dihydroxybutyl)guanine [bcvtp and (s)-dhbgtp], 9-(4-hydroxybutyl)guanine (hbgtp), and 9-(2-hydroxyethoxymethyl)guanine (acvtp) were investigated for their effects on partially purified dna polymerases of herpes simplex virus type 1 and type 2 (hsv-1 and hsv-2) as well as cellular dna polymerase alpha of calf thymus and vero cells. the triphosphates of the four analogs were all competitive inhibitors ... | 1986 | 3012321 |
| specific inhibition of herpesvirus ribonucleotide reductase by synthetic peptides. | ribonucleotide reductase is an essential enzyme for dna synthesis in all prokaryotic and eukaryotic cells; it catalyses the reductive conversion of ribonucleotides to deoxyribonucleotides. several herpesviruses including herpes simplex virus type 1 (hsv-1), hsv-2, pseudorabies virus (prv), equine herpesvirus type 1 (ehv-1) and epstein-barr virus (ebv) have been found to induce novel ribonucleotide reductase activities. there is evidence that the hsv-1 ribonucleotide reductase activity is virus-e ... | 1986 | 3012359 |
| analysis of dna from recurrent genital herpes simplex virus isolates by restriction endonuclease digestion. | in a blind study, dna from 40 clinical isolates of herpes simplex viruses was analyzed by restriction endonucleases to determine whether serial isolates from an individual patient could be identified and whether exogenous reinfection occurred within this population. five of the 40 isolates served as controls. based on restriction patterns obtained following bam h1 cleavage of dna, 35 isolates were assigned to 15 patients. isolates from two patients displayed variation in the electrophoretic mobi ... | 1986 | 3012807 |
| cellular immunity to vaccinations and herpesvirus infections after bone marrow transplantation. | the cellular immune response to herpesviruses was studied in 46 recipients of marrow grafts (23 autologous, 23 allogeneic). that study was performed in vitro by evaluating the degree of lymphocyte proliferative responses to herpes simplex virus (hsv), cytomegalovirus (cmv), and varicella zoster virus (vzv). no primary infections with any of those viruses were noted after bone marrow transplantation (bmt). the incidence of active infection in seropositive patients was significantly lower after au ... | 1986 | 3012833 |
| efficacy of the acyclic guanosine analog buciclovir [(r)-9-(3,4-dihydroxybutyl)guanine] in experimental genital herpes. | the efficacy of the anti-herpesvirus drug buciclovir [(r)-9-(3,4-dihydroxybutyl)guanine] was investigated in guinea pigs and mice infected intravaginally with herpes simplex virus type 2. topical treatment initiated early after infection was efficacious, in contrast to topical treatment delayed 24 h or more. systemic treatment of infected mice could not prevent the spread of virus to the brain and mortality. systemically administered buciclovir had an effect in guinea pigs, even after delayed on ... | 1986 | 3013082 |
| comparison of susceptibilities of varicella-zoster virus and herpes simplex viruses to nucleoside analogs. | the susceptibilities of varicella-zoster virus (vzv) and herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) to 17 nucleoside analogs were compared by a plaque reduction assay with human embryonic lung fibroblast cells. the susceptibility of vzv to certain nucleoside analogs was different from that of hsv-1. against vzv the 5-halogenovinyl-arabinosyluracils were the most potent of the compounds tested. | 1986 | 3013088 |
| evaluation of five cell types for the isolation of herpes simplex virus. | five cells were evaluated in a comparative analysis for sensitivity, specificity, and rapidity in detecting the presence of herpes simplex virus hsv-1 and hsv-2. included in this study were human embryonic kidney (hek), rabbit kidney (rk), mrc-5, mink lung (ml), and microtus agrestis (umma). a total of 274 specimens from genital, throat, skin, or other sources that were submitted for hsv isolation were used in the study. the sensitivity of the different cells was assessed by the total number of ... | 1986 | 3013496 |
| rapid identification of herpesvirus simiae (b virus) dna from clinical isolates in nonhuman primate colonies. | a rapid, simple technique, based on restriction endonuclease analysis of radioactively labeled infected cell dna, is described for identification of herpesvirus simiae (b virus) infection in clinical isolates from nonhuman primates. isolates can be screened within 2-3 days from the time of collection of a specimen from a suspect lesion to final viral identification. isolates were obtained from eight animals with suspected b virus infections. the results indicated the presence of b virus in each ... | 1986 | 3013916 |
| a filamentous distribution for the herpes simplex virus type 2-encoded major dna-binding protein. | monoclonal antibodies reacting with the herpes simplex virus (hsv)-encoded major dna-binding protein defined an intracellular filamentous network. this network was associated predominantly with the infected cell nucleus and occurred in cells infected with hsv type 2. it did not co-distribute with microfilaments, microtubules or intermediate filaments, and dna synthesis was required for its formation. we suggest explanations for the occurrence and function of this novel filamentous network struct ... | 1986 | 3014046 |
| characterization of the tumor-associated 38-kd protein of herpes simplex virus type 2. | the bglii n dna fragment of herpes simplex virus type 2 (hsv 2), which is capable of oncogenically transforming cells in vitro, encodes a 37,800-dalton (38-kd) protein that has been seroepidemiologically associated with uterine cervical carcinoma. polyclonal monospecific antiserum was produced against electrophoretically purified 38 kd from hsv 2-infected cells and used to identify antigenic and biochemical characteristics of the protein as well as to probe transformed cells for the expression o ... | 1986 | 3014138 |
| comparative studies of host-cell reactivation, cellular capacity and enhanced reactivation of herpes simplex virus in normal, xeroderma pigmentosum and cockayne syndrome fibroblasts. | host-cell reactivation (hcr) of uv-irradiated herpes simplex virus type 2 (hsv-2), capacity of uv-irradiated cells to support hsv-2 plaque formation and uv-enhanced reactivation (uver) of uv-irradiated hsv-2 were examined in fibroblasts from 4 patients with cockayne syndrome (cs), 5 with xeroderma pigmentosum and 5 normals. all uv-survival curves for hsv-2 plaque formation showed 2 components. hcr was similar to normal for the xp variant strain and the 2 cs strains tested, but substantially redu ... | 1986 | 3014327 |
| [comparative study of the effectiveness of acycloguanosine and phosphonoacetic acid in herpetic infection in cell cultures]. | a comparative study of cytotoxicity and antiherpes activity of acycloguanosine (acg) of the soviet and american manufacture and a soviet preparation of phosphonoacetic acid (paa) for herpes simplex virus types 1 and 2 (hsv-1 and hsv-2) was carried out in primary and continuous cell cultures. the acg preparation was shown not to be inferior in maximal tolerated concentration, minimal inhibiting concentration, and chemotherapeutic index (cti) to american drug. zovirax (zvr), in relation to both he ... | 1986 | 3014751 |
| activities of 1-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)-5-iodocytosine and its metabolites against herpes simplex virus types 1 and 2 in cell culture and in mice infected intracerebrally with herpes simplex virus type 2. | as measured by plaque and yield reduction assays, several metabolites of 1-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)-5-iodocytosine (fiac) were highly active against herpes simplex virus types 1 and 2. these metabolites included the 2'-deoxy-2'-fluoroarabinosyl derivatives of 5-iodouracil (fiau), cytosine (fac), uracil (fau), and thymine (fmau). in mice inoculated intracerebrally with herpes simplex virus type 2, the relative order of potency of these compounds and licensed antiviral drugs was ... | 1986 | 3015003 |
| effect of interferon on herpes simplex virus replication in murine macrophage-like cell lines. | the effect of interferon on the replication of herpes simplex virus types 1 and 2 was studied in two murine macrophage-like cell lines which differ in their ability to synthesize interferon. higher titers of herpes simplex virus type 1 occurred in pu5-1.8 cell cultures where interferon was not produced than in j774a.1 cell cultures where low amounts of interferon were produced. herpes simplex virus type 2 replicated poorly in both types of cell cultures. interferon synthesis was induced in j774a ... | 1986 | 3015020 |
| herpes simplex virus type 1 and 2 in the adrenal glands: replication and histopathology. | the adrenal glands were shown to be the most severely infected organs in the early phase of hsv-1 infections (up to 10 days p.i.) after i.p. infections in mice. virus could be isolated from the adrenal glands as early as one hour after infection with pathogenic and apathogenic strains. infection of the adrenal glands is a result of viremia. the content of hsv-1 (5 strains) was much higher in the adrenals than in spleen and liver. it peaked at 3-4 days p.i. compared to 1-2 days in spleen and live ... | 1986 | 3015081 |
| a latent infection of herpes simplex virus type 2 in a human neuroblastoma cell line imr-32. | human neuroblastoma (imr-32) cells were infected with herpes simplex virus type 2 (hsv-2) at a multiplicity of infection (moi) of 2 plaque-forming units (pfu)/cell and were cultured at 40 degrees c for 14 days. then neither infectious virus particles nor virus capsids were detected in these cells whereas the presence of virus-specific antigens was observed by immunofluorescent antibody staining technique in 16.9 +/- 3.2 per cent of the infected cell population. when the cultivation temperature w ... | 1986 | 3015082 |
| transformation of dog embryo kidney cells by human herpesviruses. | the infection of dog embryo kidney (dek) cells with herpes simplex virus type 2 (hsv-2) or human cytomegalovirus (hcmv) led to the development of transformed cell lines. rapidly dividing dek cells with unlimited division potential exhibited growth in 2% serum, contained nuclear virus antigens, and formed small (+/- 0.2 mm) colonies in 0.3% agarose. immortal cell lines showing the same transformation properties were also obtained after transfection with purified hsv-2 or hcmv dna. these results c ... | 1986 | 3015422 |
| prevalence of htlv iii/lav and other viral antibodies in normal and at risk populations. | the prevalence of htlv iii/lav antibody has been studied in six groups of population: healthy subjects, a virological laboratory staff, prisoners, drug addicts, patients with lymphoadenopathy-associated syndrome (las) and patients with aids. prevalences and levels in the same groups of hbsag and cmv, hsv 1 and hsv 2 antibodies have been studied, too. as expected, the prevalence of htlv iii/lav antibody increases in the six groups with the increasing of the risk while the prevalences of the other ... | 1986 | 3030363 |
| viral alkaline nuclease in intranuclear dense bodies induced by herpes simplex infection. | the distribution of the herpes simplex virus alkaline nuclease (hsv dnase) in rabbit fibroblast cells infected with hsv type 1 or 2 (hsv-1 or hsv-2) has been examined with the aid of immunocytochemical techniques using gold particles as markers. hsv dnase was found to be accumulated within infected nuclei as early as 2.5 hr post-infection. labeled antibody to hsv dnase subsequently increased in all nuclei after 7 hr and 17 hr. at all times post-infection the virus induced nuclear dense bodies we ... | 1986 | 3032315 |
| herpes simplex virus gene products involved in the induction of chromosomal aberrations. | the effect of short-term herpes simplex virus type 1 (hsv-1) infection on chromosomes of human diploid fibroblasts was examined. in addition to chromosomal breaks, gaps and pulverization, three kinds of cytogenetic damage (double minutes, polyploidy and endoreduplication) not yet reported following productive infection with hsv or other animal viruses were frequently observed. consistent with previous studies suggesting that the expression of immediate-early and/or early viral gene products is r ... | 1986 | 3033932 |
| herpes-related polypeptides from a human cervical carcinoma cell line. | using antiserum against herpes simplex virus type 2 (hsv-2) infected cells, eight polypeptides with similar molecular weights could be immunoprecipitated from the nearly diploid, human cervical carcinoma cell line c4ii and from hsv-2 transformed hamster and mouse cells. only two of these polypeptides corresponded to those from hsv-2 infected cells, including the putative hsv-2 transformation-related 35k protein. partial proteolytic cleavage products of the immunoprecipitated 35k polypeptides fro ... | 1986 | 3017554 |
| cryosurvival of herpes simplex virus-2 during cryopreservation of human spermatozoa. | herpes simplex virus (hsv) is a sexually transmitted agent which has potential association with cervical cancer, as well as with high morbidity and mortality in perinatal infection. its incidence is estimated just after gonorrhea and chlamydia trachomatis infections. cryobanking of human semen is accepted worldwide in therapeutic use for both husband and donor. serious consequences could follow the clinical applications of cryobanked human semen in insemination, if some ejaculates contain hsv an ... | 1986 | 3017635 |
| failure to induce cervical cancer in mice by long-term frequent vaginal exposure to live or inactivated herpes simplex viruses. | c57 mice aged 8-10 weeks in groups of 50 each received vaginal cotton pellets soaked in lysates of hep-2 cells, either mock-infected or infected with herpes simplex virus i, herpes simplex virus 2, and highly attenuated recombinant viruses 5 times a week for 89 to 114 weeks. an untreated group was also included. the mock-infected and some of the infected cell lysates were exposed to ultraviolet light at a dose sufficient to inactivate virus. smears of exfoliated vaginal cells collected once a mo ... | 1986 | 3017867 |
| analyses of transplanted murine tumors for hsv dna sequences. | meignier et al. (1986) report the results of exposure of c57bl/6ncr mice to vaginal plugs containing live or inactivated herpes simplex virus 1 or 2 (hsv-1 or hsv-2) or recombinant viruses 5 times a week for up to 114 weeks. genital organs showing abnormalities were transplanted into nude mice. of 33 transplants, 13 produced subcutaneous tumors in nude mice and 12 were subsequently transplanted into c57bl/6ncr mice. we report that the dna extracted from coded tumor tissues of nude mice and from ... | 1986 | 3017868 |
| zosteriform spread of herpes simplex virus type 2 genital infection in the guinea-pig. | a model of herpes simplex virus type 2 (hsv-2) infection was developed in the guinea-pig that permits investigation of the role of neural spread of virus in the pathogenesis of genital skin disease. after hsv-2 inoculation of an abraded area lateral to the external genital skin, virus was first detected in the ipsilateral (to the inoculation site) peripheral nerves or genital skin on day 2 or 3 post-inoculation followed by detection in the ipsilateral dorsal root ganglia and spinal cord on day 3 ... | 1986 | 3018127 |
| genetically determined difference in the antiviral action of alpha/beta interferon in cells from mice resistant or susceptible to herpes simplex virus type 2. | resistance of mice to infection with herpes simplex virus type 2 (hsv-2) is genetically determined. embryonic cells from susceptible balb/c and resistant c57bl/6 mice were equally sensitive to infection with hsv-2 as judged by plaque area, plaquing efficiency, endpoint titration and virus yield. cells from c57bl/6 mice showed a higher sensitivity than cells from balb/c mice to the protective action of two preparations of alpha/beta interferon against challenge with hsv-2. this was evident both f ... | 1986 | 3018128 |
| delta-9-tetrahydrocannabinol enhances release of herpes simplex virus type 2. | this study was undertaken to determine the effect of micromolar concentrations of delta-9-tetrahydrocannabinol (delta-9-thc) on herpes simplex virus type 2 (hsv-2) replication in vitro. virus-infected vero cells pretreated for 24 h with 10(-5) m- or 10(-6) m-delta-9-thc yielded 100-fold increases in infectious extracellular virus. transmission electron microscopy of drug-treated cells revealed plasma membrane dissolution, distension of the smooth and rough endoplasmic reticulum, and the appearan ... | 1986 | 3018134 |
| carbocyclic analogues of 5-halocytosine nucleosides. | carbocyclic analogues of 5-halocytosine nucleosides were prepared by direct halogenation of the carbocyclic analogues of cytidine, 2'-deoxycytidine, 3'-deoxycytidine, or ara-c. the 5-chloro and 5-bromo derivatives of the cytidine (carbodine) and of the 2'-deoxycytidine analogues and the 5-iodo derivatives of all four of the cytosine nucleoside analogues were prepared. all of the c-5-halocytosine nucleosides, as well as the parent c-cytosine nucleosides, were tested against a strain of herpes sim ... | 1986 | 3018246 |
| herpes simplex virus suppression of human endothelial matrix protein synthesis is independent of viral protein synthesis. | protein synthesis was determined in cultures of human umbilical cord vein endothelial cells (ec) infected with either herpes simplex type 1 (hsv-1) or type 2 (hsv-2). monolayers were infected for 1 hour with either 5 or 20 infectious virus particles per ec (multiplicity of infection of 5 or 20). at different times after infection, infected and noninfected cultures were pulsed with either 5 mu ci/ml of [14c]proline or 25 mu ci/ml of [35s]methionine for 1 or 2 hours. autoradiograms of sodium dodec ... | 1986 | 3018360 |
| synergistic inhibitory effect of acyclovir and human native beta-interferon on the growth of herpes simplex virus type 2 in human embryo fibroblast cell cultures. | 1986 | 3018459 | |
| antiherpetic effects of a human alpha interferon analog, ifn-alpha con1, in hamsters. | the efficacy of a novel consensus form of human alpha interferon designated ifn-alpha con1 was evaluated against herpesvirus infections in vitro and in vivo. at comparable antiviral concentrations, natural lymphoblastoid ifn, ifn-alpha con1, the molecular subtype ifn-alpha, and the hybrid ifn-alpha ad(bgl) obtained by recombinant dna methods conferred similar protection against herpes simplex virus type 1 and type 2 (hsv-2) infections of human cells in vitro. whereas 7 x 10(5) u of ifn-alpha ad( ... | 1986 | 3019238 |