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biological response of free-living mouse mus spretus from doñana national park under environmental stress based on assessment of metal-binding biomolecules by sec-icp-ms.a metallomic approach based on the use of size-exclusion chromatography (superdex-75) with inductively coupled plasma mass spectrometry (icp-ms) detection is combined with anion or cation exchange chromatography to characterize the biological response of the free-living mouse mus spretus. the approach has been applied to contaminated and non-contaminated areas from doñana national park (southwest spain) and the surroundings. several areas affected by differential contamination from mining, indus ...201223053170
direct evidence of allele-specific binding of ctcf and mecp2 to tsix in a hprt-deficient female f₁ hybrid mouse cell line.mammalian dosage compensation requires silencing of one of the two x chromosomes in females and is controlled by the x inactivation center (xic). xic contains many of the regulatory elements for the mutual interplay of x-inactive specific transcript (xist) and tsix, the antisense counterpart of xist. the regulatory elements control x chromosome inactivation (xci) via the formation of dna-dna and dna-protein complexes with cis- and trans-acting factors. however, the process-dependent regulation o ...201222889989
nasal bone shape is under complex epistatic genetic control in mouse interspecific recombinant congenic strains.genetic determinism of cranial morphology in the mouse is still largely unknown, despite the localization of putative qtls and the identification of genes associated with mendelian skull malformations. to approach the dissection of this multigenic control, we have used a set of interspecific recombinant congenic strains (ircs) produced between c57bl/6 and mice of the distant species mus spretus (seg/pas). each strain has inherited 1.3% of its genome from seg/pas under the form of few, small-size ...201222662199
obesity in bsb mice is correlated with expression of genes for iron homeostasis and leptin.we searched for genes whose alleles cause obesity and novel pathways correlated with obesity.200414981211
a new locus for resistance to gamma-radiation-induced thymic lymphoma identified using inter-specific consomic and inter-specific recombinant congenic strains of mice.mice of the c57bl/6j inbred strain develop thymic lymphomas at very high frequency after acute gamma-irradiation, while mice of several inbred strains derived from the wild progenitor of the mus spretus species and their f1 hybrids with c57bl/6j appear extremely resistant. analysis of the genetic determinism of the gamma-radiation-induced thymic lymphoma (ritl) resistance with the help of inter-specific consomic strains (ics), which carry a single introgressed mus spretus chromosome on a c57bl/6 ...200212242666
reference blood chemistry values throughout the lifespan of m. spretus.in the present study, blood chemistry values for mus spretus are reported and analyzed with respect to age and gender. cholesterol values differ significantly (p = 0.01) between young (2 to 6 months) and aged (25 to 31 months) m. spretus, and direct or conjugated bilirubin values differ significantly (p = 0.01) between male and female animals. these findings indicate that cholesterol concentrations in m. spretus change with age and that direct bilirubin values differ depending on gender.199912086456
differential effects of the kitw mutation with other kit allelic combinations on male sterility among inter- or intrasubspecific hybrids in mice.the combination of the kitw mutation and kits allele from mus spretus leads to male hybrid sterility. the effects of other combinations between kitw and kitm from mus m. molossinus or kitn from mus m. musculus on male reproductive ability were examined in this study. the kitw/kitm and kitw/kitn males were fertile and showed the normal pattern of spermatogenesis in most seminiferous tubules. there were two amino acid substitutions in the protein deduced from the cdna sequence coded by the kitm al ...200211989784
genetic influence on phenotypic differentiation in adult hippocampal neurogenesis.regulation of adult hippocampal neurogenesis has different regulatory levels, including cell proliferation, survival and differentiation. cell proliferation and survival are differentially influenced by inheritable traits and the genetic background determines which regulatory levels of adult hippocampal neurogenesis are preferentially involved in a neurogenic response to environmental stimuli. we here compared baseline adult neurogenesis in wild-derived strain mus spretus and three inbred labora ...200211947932
developmental study of the different effects on the hybrid sterility of kit and kitw-v alleles paired with kit from mus spretus.the combination of the kitw or kitw-n mutant alleles and kits from mus spretus results in male hybrid sterility with small testes. in the present study, reproduction of the combination between kitw-v and kits alleles was examined. the kitw-v/kits male was fertile and the histologic structure was normal; the seminiferous tubules showed all of the normal stages of spermatogenesis. the postnatal development of the testis at 8, 12, 16 and 20 days was also studied in the fertile +kit/+kit and kitw-v/ ...200111576178
male hybrid sterility of mice with the genomic region of the kitw mutation and the kits allele from mus spretus.two congenic strains, c57bl-kitw and c57bl-kits, were generated. the kitw allele originated from strain wb-kitw and the kits allele from mus spretus. the kitw/kits males showed hybrid sterility with small testes, but the females were fertile. the development of the seminiferous tubules of kitw/kits males stopped before the spermatocyte stage and they were almost free of sperm. the kit gene is located at position 42 on chromosome 5. we investigated in the c57bl-kits congenic strain which part of ...200111521507
biochemical bio markers of pollution in algerian mouse (mus spretus) to assess the effects of the aznalcóllar disaster on doñana park (spain).on april 25, 1998, a tailings dam of the aznalcóllar pyrite mine partially collapsed and released to the guadiamar river acidic water (ph < 3) and mud containing toxic metals (fe, zn, pb, as, cu, sb, co, tl, bi, cd, ag, hg, sr), threatening the doñana national park, a spanish wildlife reserve. to assess possible biological effects in terrestrial ecosystems, biochemical biomarkers have been assayed for the first time in algerian mice (mus spretus), a non-protected and free-living species, from ...200123886110
complete cdna sequence, genomic structure, and chromosomal localization of the lpa receptor gene, lpa1/vzg-1/gpcr26.the lpa1/gpcr26 locus encodes the first cloned and identified g-protein-coupled receptor that specifically interacts with lysophosphatidic acid. a murine full-length cdna of size consistent with that seen on northern blots (3.7 kb) was determined using 3' rapid amplification of cdna ends. analysis of genomic clones revealed that the gene is divided into five exons, with one intron inserted in the coding region for transmembrane domain vi and one exon encoding the divergent 5' sequence in another ...19989721207
mus spretus line-1s in c57bl/6j map to at least two different chromosomes. 19989680394
telomere length regulation during postnatal development and ageing in mus spretus.telomere shortening has been causally implicated in replicative senescence in humans. to examine the relationship between telomere length and ageing in mice, we have utilized mus spretus as a model species because it has telomere lengths of approximately the same length as humans. telomere length and telomerase were analyzed from liver, kidney, spleen, brain and testis from >180 m.spretus male and female mice of different ages. although telomere lengths for each tissue were heterogeneous, signif ...19979224604
meiotic abnormalities in hybrid mice of the c57bl/6j x mus spretus cross suggest a cytogenetic basis for haldane's rule of hybrid sterility.light- and electron-microscopic analyses of chromosomal pairing and recombination in f1 and first-backcross generation mice of the c57bl/6j x mus spretus cross revealed a variety of meiotic irregularities that could contribute to meiocyte loss and infertility. pachytene anomalies included univalency, partially paired bivalents, homolog-length inequalities, nonhomologous pairing, and associations of asynapsed autosomal segments with the x chromosome. these phenomena were most prevalent in f1 male ...19938500353
evolution of the mammalian g protein alpha subunit multigene family.heterotrimeric guanine nucleotide binding proteins (g proteins) transduce extracellular signals received by transmembrane receptors to effector proteins. the multigene family of g protein alpha subunits, which interact with receptors and effectors, exhibit a high level of sequence diversity. in mammals, 15 g alpha subunit genes can be grouped by sequence and functional similarities into four classes. we have determined the murine chromosomal locations of all 15 g alpha subunit genes using an int ...19921302014
paternal inheritance of mitochondrial dna in mice.for nearly 20 years it has been assumed on the basis of low-resolution experiments that mitochondrial (mt)dna, in contrast to the genes in the nucleus, has an exclusively maternal mode of inheritance in animals. using the polymerase chain reaction, paternally inherited mtdna molecules have now been detected in mice at a frequency of 10(-4), relative to the maternal contributions. these mice were hybrids between two inbred strains (c57bl/6j and mus spretus) whose mtdnas can be distinguished easil ...19911857422
hst-3: an x-linked hybrid sterility gene.a gene, hst-3, responsible for sterility in f1 males from crosses between mus spretus and laboratory strains of mice such as c57bl/6, has been localized on the distal part of the x chromosome, using both dna probes and biochemical markers on a panel of f1(c57bl/6 x seg) x c57bl/6 backcross males. this gene may be a model for studying mammalian hybrid sterility.20102272506
"new" allotypes of the murine gamma 1 immunoglobulins.spe wild-derived inbred mice belonging to mus spretus species can produce antibodies against gamma 1 isotypic determinants, common to european mice belonging to the mus subgenus, with the exception of the mus spretus species. they are also able to produce antibodies which define a "new" igh-4 allotypic specificity (designated igh-4.4).19892475767
mta, the maternally transmitted antigen, is determined jointly by the chromosomal hmt and the extrachromosomal mtf genes.mus spretus from four stocks, originating in spain, portugal, and morocco, were tested for the maternally transmitted antigen, mta. all expressed a variant form not found in other species of mice. analysis of appropriate crosses with inbred mice showed that the spretus form of mta is determined by a new allele, c, of the hmt gene. the hmtc allele has been isolated in coupling with four different h-2 haplotypes. it is possible to raise ctl specific for the spretus form of mta. the maternally tran ...19863511170
the gene encoding the ia antigen-associated invariant chain (ii) is not linked to the h-2 complex.the invariant (ii) chain of murine ia antigens is associated with the intracellular but not the cell-surface forms of the a alpha:a beta and e alpha:e beta ia complexes. due to its unique subcellular localization, ii has been postulated to play a part in the assembly or intracellular transport of the ia alpha:beta complexes, which function in immune recognition. a more general role for ii in the transport of other cell proteins has also been suggested. because of the unusual subunit composition ...19836402712
long-term data from a small mammal community reveal loss of diversity and potential effects of local climate change.climate change affects distribution and persistence of species. however, forecasting species' responses to these changes requires long-term data series that are often lacking in ecological studies. we used 15 years of small mammal trapping data collected between 1978 and 2015 in 3 areas at doñana national park (southwest spain) to (i) describe changes in species composition and (ii) test the association between local climate conditions and size of small mammal populations. overall, 5 species wer ...201729492011
wild mammals as potential silent reservoirs of leishmania infantum in a mediterranean area.a molecular survey of wild mammals was performed to assess their potential as reservoirs of l. infantum. a total of 156 specimens of wild mammalian fauna were obtained for analysis from areas in catalonia with a reported incidence of canine leishmaniasis. they consisted of 124 small mammals: 35 mus spretus (muridae); 64 erinaceus europaeus (erinaceidae), 25 sciurus vulgaris (sciuridae) and 32 carnivores: 11 vulpes vulpes (canidae), 1 felis catus (felidae), 15 meles meles, 4 martes foina and 1 mu ...202031901603
nuclear response to divergent mitochondrial dna genotypes modulates the interferon immune response.mitochondrial oxphos generates most of the energy required for cellular function. oxphos biogenesis requires the coordinated expression of the nuclear and mitochondrial genomes. this represents a unique challenge that highlights the importance of nuclear-mitochondrial genetic communication to cellular function. here we investigated the transcriptomic and functional consequences of nuclear-mitochondrial genetic divergence in vitro and in vivo. we utilized xenomitochondrial cybrid cell lines conta ...202033031404
mus musculus populations in western australia lack vkorc1 mutations conferring resistance to first generation anticoagulant rodenticides: implications for conservation and biosecurity.humans routinely attempt to manage pest rodent populations with anticoagulant rodenticides (ars). we require information on resistance to ars within rodent populations to have effective eradication programs that minimise exposure in non-target species. mutations to the vkorc1 gene have been shown to confer resistance in rodents with high proportions of resistance in mice found in all european populations tested. we screened mutations in mus musculus within western australia, by sampling populati ...202032970676
out of africa: demographic and colonization history of the algerian mouse (mus spretus lataste).north africa is now recognized as a major area for the emergence and dispersal of anatomically modern humans from at least 315 kya. the mediterranean basin is thus particularly suited to study the role of climate versus human-mediated changes on the evolutionary history of species. the algerian mouse (mus spretus lataste) is an endemic species from this basin, with its distribution restricted to north africa (from libya to morocco), iberian peninsula and south of france. a rich paleontological r ...201929795180
development of assisted reproductive technologies for mus spretus†.the genus mus consists of many species with high genetic diversity. however, only one species, mus musculus (the laboratory mouse), is common in biomedical research. the unavailability of assisted reproductive technologies (arts) for other mus species might be a major reason for their limited use in laboratories. here, we devised arts for mus spretus (the algerian mouse), a commonly used wild-derived mus species. we found that in vitro production of m. spretus embryos was difficult because of lo ...202032990726
placental effects on the maternal brain revealed by disrupted placental gene expression in mouse hybrids.the mammalian placenta is both the physical interface between mother and fetus, and the source of endocrine signals that target the maternal hypothalamus, priming females for parturition, lactation and motherhood. despite the importance of this connection, the effects of altered placental signalling on the maternal brain are insufficiently studied. here, we show that placental dysfunction alters gene expression in the maternal brain, with the potential to affect maternal behaviour. using a cross ...202031937228
placental genotype affects early postpartum maternal behaviour.the mammalian placenta is a source of endocrine signals that prime the onset of maternal care at parturition. while consequences of placental dysfunction for offspring growth are well defined, how altered placental signalling might affect maternal behaviour is unstudied in a natural system. in the cross between sympatric mouse species, mus musculus domesticus and mus spretus, hybrid placentas are undersized and show misexpression of genes critical to placental endocrine function. using this cros ...201931598302
automated nuclear cartography reveals conserved sperm chromosome territory localization across 2 million years of mouse evolution.measurements of nuclear organization in asymmetric nuclei in 2d images have traditionally been manual. this is exemplified by attempts to measure chromosome position in sperm samples, typically by dividing the nucleus into zones, and manually scoring which zone a fluorescence in-situ hybridisation (fish) signal lies in. this is time consuming, limiting the number of nuclei that can be analyzed, and prone to subjectivity. we have developed a new approach for automated mapping of fish signals in a ...201930717218
using the inbred mouse strain spret/eij to provide novel insights in inflammation and infection research.inbred mouse strains derived from the species mus spretus have been very informative in the study of certain gene polymorphisms in inflammation and infection. based on our interest in sepsis, we used spret/eij mice and mapped several critical loci that are linked to sensitivity to cytokine-induced inflammation and endotoxemia. these studies were based on prominent phenotypes that have never been observed in strains derived from mus musculus and we mapped them at a resolution that enables us to d ...201829947962
a high quality genome for mus spicilegus, a close relative of house mice with unique social and ecological adaptations.genomic data for the closest relatives of house mice (mus musculus species complex) are surprisingly limited. here, we present the first complete genome for a behaviorally and ecologically unique member of the sister clade to house mice, the mound-building mouse, mus spicilegus using read cloud sequencing and de novo assembly we produced a 2.50 gbp genome with a scaffold n50 of 2.27 mbp. we constructed >25 000 gene models, of which the majority had high homology to other mus species. to evaluate ...201829794166
genetic mapping of species differences via in vitro crosses in mouse embryonic stem cells.discovering the genetic changes underlying species differences is a central goal in evolutionary genetics. however, hybrid crosses between species in mammals often suffer from hybrid sterility, greatly complicating genetic mapping of trait variation across species. here, we describe a simple, robust, and transgene-free technique to generate "in vitro crosses" in hybrid mouse embryonic stem (es) cells by inducing random mitotic cross-overs with the drug ml216, which inhibits the dna helicase bloo ...201829563231
transcriptome signatures of p,p´-dde-induced liver damage in mus spretus mice.the use of ddt (1,1,1-trichloro-2,2-bis(p-chlorophenyl) ethane) in some countries, although regulated, is contributing to an increased worldwide risk of exposure to this organochlorine pesticide or its derivative p,p'-dde [1,1-dichloro-2,2-bis(p-chlorophenyl) ethylene]. many studies have associated p,p'-dde exposure to type 2 diabetes, obesity and alterations of the reproductive system, but their molecular mechanisms of toxicity remain poorly understood. we have addressed this issue by using com ...201829554563
molecular survey for cyst-forming coccidia (toxoplasma gondii, neospora caninum, sarcocystis spp.) in mediterranean periurban micromammals.rodents and other micromammals constitute important reservoirs of infectious diseases; their role in the life cycle of apicomplexan parasites such as toxoplasma gondii, neospora caninum, and sarcocystis spp. still needs clarification. in the present study, we analyzed by pcr and sanger sequencing methods the presence of specific parasite dna within brain and heart tissues of 313 individuals of five synanthropic small mammal species (apodemus sylvaticus, mus spretus, m. musculus, rattus rattus, a ...202032588173
pedro ximenez sun-dried grape must: a dietary supplement for a healthy longevity.polyphenols in red wine are bioactive compounds with positive effects on health and disease prevention. white grape musts and wines have a lower concentration of phenolic compounds compared to the red ones and are therefore considered less beneficial to health. in andalucía, a region located in the south west of spain, pedro ximenez white grapes are desiccated under the sun for a week before they are pressed and the juice (must) is obtained. this ancient procedure increases the variety and conte ...202032374335
protective effect of pedro-ximénez must against p,p'-dde-induced liver damages in aged mus spretus mice.aging is characterized by deterioration of biomolecules and impaired stress responses that make the elderly especially vulnerable to environmental pollutants. the pesticide p,p'-dde is a ddt derivative that generates great concern because of its wide distribution and its harmful effects on both human health and the environment. we analyzed here the biological responses elicited by p,p'-dde exposure in the liver of aged mus spretus mice. data demonstrate that the elderly constitute a population e ...202031765701
redox and global interconnected proteome changes in mice exposed to complex environmental hazards surrounding doñana national park.natural environments are receiving an increasing number of contaminants. therefore, the evaluation and identification of early responses to pollution in these complex habitats is an urgent and challenging task. doñana national park (dnp, sw spain) has been widely used as a model area for environmental studies because, despite its strictly protected core, it is surrounded by numerous threat sources from agricultural, mining and industrial activities. since many pollutants often induce oxidative s ...201931158671
population effects of heavy metal pollution in wild algerian mice (mus spretus).heavy metal mining is one of the largest sources of environmental pollution. the analysis of different types of biomarkers in sentinel species living in contaminated areas provides a measure of the degree of the ecological impact of pollution and is thus a valuable tool for human and environmental risk assessments. in previous studies we found that specimens from two populations of the algerian mice (mus spretus) living in two abandoned heavy metal mines (aljustrel and preguiça, portugal) had hi ...201930639867
examination and reconstruction of three ancient endogenous parvovirus capsid protein gene remnants found in rodent genomes.parvovirus-derived endogenous viral elements (eves) have been found in the genomes of many different animal species, resulting from integration events that may have occurred from more than 50 million years ago to much more recently. here, we further investigate the properties of autonomous parvovirus eves and describe their relationships to contemporary viruses. while we did not find any intact capsid protein open reading frames in the integrated viral sequences, we examined three eves that were ...201930626673
molecular investigation of vector-borne parasites in wild micromammals, barcelona (spain).information about the role of micromammals in the epidemiology of leishmania infantum and babesia sp. in southern europe is scarce. wild micromammals were captured in natural areas in the barcelona province (ne spain) in 2011 and analysed by real-time pcr for the presence of dna of piroplasmida (n = 253) and leishmania infantum (n = 166). the wood mouse (apodemus sylvaticus) was the most abundant species, but all specimens tested negative for l. infantum, for which two out of 15 (13.3%) white-to ...201829943318
alterations in oxidative responses and post-translational modification caused by p,p´-dde in mus spretus testes reveal cys oxidation status in proteins related to cell-redox homeostasis and male fertility.the major derivate of ddt, 1,1-dichloro-2,2-bis (p-chlorophenyl) ethylene (p,p´-dde), is a persistent pollutant previously associated with oxidative stress. additionally, p,p´-dde has been linked to several metabolic alterations related to sexual function in rodents. in this study, we analysed the effects of a non-lethal p,p´-dde dose to mus spretus mice in testes, focusing on oxidative damage to biomolecules, defence mechanisms against oxidative stress and post-translational protein modificatio ...201829723838
dietary se supplementation partially restores the redox proteomic map of m. spretus liver exposed to p,p'-dde.the toxicity of p,p'-dichlorodiphenyldichloroethylene (p,p'-dde), a contaminant and metabolite derivative of ddt [1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane] is partially mediated by reactive oxygen species. protein cysteine-based regulatory switches and subsequent alterations of the overall hepatic metabolism are triggered by p,p'-dde through the disruption of the cellular redox status. the consequences are reproductive impairment, metabolic disorders, diabetes, neurotoxicity and cancer. in ...201829481894
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