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cytochemical demonstration of esterases in peripheral blood leukocytes.cytochemical methods for alpha naphthyl acetate esterase and chloroacetate esterase have been used to identify human monocytes and granulocytes. in this study, a standard procedure for staining alpha naphthyl acetate and chloroacetate esterase activities was modified by extending the range of ph of the incubation mixture and the duration of staining and was applied to cat, dog, goat, guinea pig, hamster, human, pig, rabbit, rat, and sheep leukocytes. the results for both enzymes showed (1) incub ...197825603
activity of gamma-glutamyl transferase of sheep cerebral cortex with respect to amino acids and glutathione.gamma-glutamyl transferase fron sheep brain cortex capillaries was studied from the point of view of transport of aminoacids across blood brain barrier. excess substrate inhibition was competitive and observed both with donor (glutathione) and various acceptors (methionine, alanine, tryptophan) but not with arginine. excess glutathione inhibition of transfer reaction is concomitant with an increase of total reaction (transfer + hydrolysis + autotranspeptidation). with regard to aminoacids, the g ...197825678
on the isolated perfused sheep ovary as a model for the study of ovarian function. 197828253
experimental cross infections of fasciola hepatica in lambs and calves.the effects of experimental infections with fasciola hepatica of ovine and bovine origin in homologous and heterologous hosts and in uninfected controls were compared; groups comprised 5 animals each. the effects of the infections were monitored by biweekly determinations of packed cell volumes (pcv), serum glutamic oxaloacetic transaminase (sgot), serum glutamic pyruvic transaminase (sgpt), gamma glutamyl transpeptidase (ggt), serum iron, bilirubin levels, alkaline phosphatase (alp) and total s ...197828389
serum gamma-glutamyltransferase as a measure of sporidesmin-induced liver damage in sheep. 197827740
intracellular buffering.in the quantification of buffering the distinct roles of bicarbonate and of other buffers must be taken into account. the determination of the total non-bicarbonate buffer value, betaa, in intact tissues is complicated by active ph regulation and by heterogeneity of cytoplasm with respect to betaa, while heterogeneity with respect to ph in vivo could cause errors in estimates made with homogenates. available estimates of betaa are discussed, as are the individual contributions of proteins, dipep ...197827854
the pharmacologic inhibition of premature labor.oxytocin, elevated estrogen-progesterone ratio, fetal corticosteroids, prostaglandins, catecholamines, and changes in uterine blood flow have all been implicated as triggers of labor. in approximately one-third of cases of threatened premature labor contractions stop spontaneously. thus placebo-controlled randomized trials of any new drug for inhibition of premature labor are necessary, as the spontaneous cessation of contractions always favors the claimed therapeutic efficacy. alcohol inhibits ...197828500
development of neurohumoral control of fetal, neonatal, and adult cardiovascular functions.neurohumoral control of fetal, neonatal, and adult cardiovascular functions have been reviewed. resting fetal heart rate remains fairly constant but neonatal heart rate declines progressively, reaching adult levels within six to eight weeks; systemic arterial pressure rises while pulmonary pressure falls to adult levels within the first week after birth. sympathetic and parasympathetic control of circulatory functions matures at different rates during fetal and neonatal development; the sympathe ...197724341
purification and properties of an nadph-dependent 21-oxo-20-hydroxysteroid reductase (17beta-aldol reductase) from sheep liver. isolation of the 20beta-glycol product.this investigation was undertaken to test the hypothesis that steroidal 20-hydroxy-21-aldehydes are intermediates in an alternative pathway of corticosteroid metabolism leading to steroidal 20,21-diols. a nadph-dependent 21-oxo-20-hydroxysteroid reductase which catalyzed the reduction of 11beta,17,20beta-trihydroxy-3-keto-4-pregnen-21-al (isocortisol) to 11beta,17,20beta,21-tetrahydroxy-4-pregnen-3-one (reichstein's compound e) was prepared from sheep liver. other steroidal 17-aldols were also g ...197824635
the effect of raised ph on pacemaker activity and ionic currents in cardiac purkinje fibers. 197828515
hemolytic assay of complement and its components from syrian hamster (mesocricetus auratus) and mongolian gerbil (mariones unguiculatus).syrian hamster sera may be assayed for hemolytic complement (c) activity by standard procedures with sensitized sheep erythrocytes as used for human sera, but mongolian gerbil sera had higher complement titers when tested with sensitized guinea pig erythrocytes as target cells. the optimal conditions for in vitro testing of hamster c were ph 7.3, ionic strength 0.15, and presence of 1 micrometer of mg2+ and 0.3 micrometer ca2+. for gerbil c the optimum ph was 8, ionic strength 0.074, and the sam ...197828965
arbovirus isolations from mosquitoes in south slovakia.in the years 1973 and 1975 mosquitoes and some other diptera (tabanidae, simuliidae, hippoboscidae) were tested for virus. 13,924 mosquitoes, 75 horseflies and 60 blackflies were processed in 1973. five strains of tahyna virus were isolated from mosquito species aedes vexans. 3,378 mosquitoes and 12 sheep keds were tested for virus in 1975. twelve strains of calovo virus were isolated from anopheles maculipennis and one strain of tahyna virus was obtained from aedes vexans mosquitoes.197828998
tissue and blood distribution of gamma-glutamyl transferase in the lamb and in the ewe.gamma-glutamyl transferase distribution was studied in 10 ewes and 10 lambs; in both groups kidney was the most active organ, followed by pancreas, liver and lungs. in kidney and liver, ggt was bound mainly to cellular structures. blood ggt activity of 48 lambs was 44 +/- 11 iu/litre and 33 +/- 7 iu/litre of 45 ewes.197830124
hemoglobin-ligand interaction in fetal and maternal sheep blood.hemoglobin-ligand interaction was studied in maternal and fetal sheep blood as a function of oxygen saturation. ph was changed by varying co2 concentration (co2 bohr effect) or by addition of naoh or hcl at constant pco2 (fixed acid bohr effect). for maternal blood, co2 bohr factor was -0.41 at 50% oxygen saturation, increasing in magnitude at lower saturation and decreasing in magnitude at higher saturation. for fetal blood, co2 bohr factor was -0.45 at 50% oxygen saturation, unchanging at lowe ...197830129
oxygen affinity in the blood of sheep.the oxygen affinity in the blood of adult dorset sheep with hbb was studied by determining several oxygen equilibrium curves under different conditions of ph and pco2 by a method that allowed strict control of ph, pco2, and hco3- concentration over the entire curve. nomograms and equations were derived that allowed the estimation of the oxygen saturation in a sample of blood in the range 0 to 100%, either from ph, pco2 and po2, or from po2 and known p50. the advantage of this approach is that no ...197830131
renal response of fetal lamb to complete occlusion of umbilical cord.the renal response of the fetal lamb to repeated complete occlusion of the umbilical cord was studied in nine chronically instrumented animals. five episodes of occlusion of the umbilical cord, each lasting for two minutes, produced a twofold rise in fetal urine osmolality and sodium, chloride, and potassium concentrations. output of urine and glomerular filtration rate remained essentially unchanged while free water clearance decreased from a control of +0.10 to -0.02 ml. per kilogram per minut ...197827986
is there a link between in regulation of intracellular sodium and intracellular ph in mammalian cardiac tissue? [proceedings]. 197832391
the energy value to sheep of mixed diets containing alkali-treated straw and ground field beans [proceedings]. 197832540
influence of mild acid hydrolysis on the antigenic properties of phase i coxiella burnetii.mild acid hydrolysis of phase i coxiella burnetii cells gradually cleaved off polysaccharide chains, which contain determinants of antigen 1, thus unmasking determinants of the diagnostically important antigen 2. this artificial phase i to phase ii conversion was reflected by changed serological activity in the complement-fixation (cf) test and by changed nonspecific phagocytosis. the product of mild acid hydrolysis can serve as a complex diagnostic preparation, because it reacts in the cf test ...197829469
hepatic ketone-body metabolism in developing sheep and pregnant ewes. 197829658
low-frequency positive pressure ventilation with extracorporeal carbon dioxide removal (lfppv-ecco2r): an experimental study.we describe a new form of mechanical pulmonary ventilation, low-frequency positive pressure ventilation with extracorporeal co2 removal (leppv-ecco2r). in a series of animal studies the rate of mechanical ventilation was 0.66, 1, 2, and 4 min-1 at a tidal volume of 3, 10, and 15 ml kg-1. we were able to maintain normal blood gases and normal lung volumes and lung mechanics even at the lowest ventilator rate with tidal volumes of 10 or 15 ml kg-1. each experiment lasted 7 hours. our data suggest ...197830341
symposium on freezing of gametes and spermatozoa-synopsis papers. 197830594
metabolism of aniline in rats, pigs and sheep. 197830604
stimulation of immune reactivity by methoxy-substituted glycerol ethers incorporated into the feed.in mice, the plaque-forming cell response to sheep red blood cells was stimulated by a mixture of methoxy-substituted glycerol ethers isolated from greenland shark liver oil and by synthetic 1-0-(2-methoxyhexadecyl)-glycerol, given in the diet. in preliminary experiments, this synthetic compound also increased the ability of parental spleen cells to induce graft-vs.-host reactions in f1 hybrid mice. glycerol ethers occur in the bone marrow fat of mammals and in the membrane phospholipids. it is ...197830635
pituitary responsiveness following infusion or repeated injections of [d-leu6, des gly-nh210]-lhrh-ethylamide in prepubertal ewes. 197830744
physico-chemical properties of mouse hepatitis virus (mhv-2) grown on dbt cell culture.some properties of a strain of mouse hepatitis virus, mhv-2, grown on dbt cells were determined using a plaque assay on the cells. viral growth was not inhibited by the presence of actinomycin d or 5-iodo-2-deoxyuridine. mhv-2 was completely inactivated by ether, chloroform, sodium deoxycholate or beta-propiolactone, but showed a moderate resistance to trypsin. heating at 56 c for 30 min did not completely abolish the virus infectivity. the virus was stable after heating at 50 c for 15 min in 1m ...197830881
effects of ovine hydatid cyst fluid in sheep before and after treatment with hydrocortisone, antihistamines and atropine.the cardiovascular and respiratory responses to scolex-free ovine hydatid cyst fluid were studied in 65 sodium pentobarbital-anesthetized sheep. the arterial blood pressure (abp), central venous pressure (cvp), stethogram and electrocardiogram were recorded. intra-venous administration of 10 ml or less hydatid fluid brought about moderate to severe fall in abp and rapid respiration or permanent respiratory cessation in majority of the animals. forty six percent of the sheep died of circulatory a ...197830932
circulatory shock in pregnant sheep. iv. fetal and neonatal circulatory responses to hypovolemia--influence of anesthesia.hemodynamic responses to hypovolemia were investigated in unanesthetized, unstressed fetal lambs and in acute fetal and neonatal preparations. the unstressed fetus tolerated twice the amount of blood loss of the acutely studied fetus or newborn lamb and with a lesser hypotension. hemodynamic behavior of the newborn lamb and fetus anesthetized with pentobarbital during hypovolemia was markedly different from that of the fetus studied under spinal anesthesia or chronically. besides tolerating grea ...197831092
progesterone: its role in the central nervous system as a facilitator and inhibitor of sexual behavior and gonadotropin release. 197731125
effect of bicarbonate and sits on alphaicl recovery in sheep cardiac purkinje fibres [proceedings]. 197831461
displacement of activator thresholds in cardiac muscle by protons and calcium ions.1. the na current threshold in sheep cardiac purkinje fibres and in frog atrium is shifted in a positive direction by protons and ca2+ ions. the titration curves for purkinje fibres are consistent with a surface potential of -18 mv at ph 7.4 and 1.8 mm-ca. 2. in purkinje fibres, the pacemaker k current activation curve, s infinity, is shifted in a positive direction by ca2+ ions. the results are consistent with a surface potential of -16 mv in normal physiological solutions. 3. the results on s ...197831463
the interactions of protons, calcium and potassium ions on cardiac purkinje fibres.1. in sheep purkinje fibres, acidosis shifts the steady-state current-voltage relation in an inward direction over a wide range of negative potentials. the reversal potential for ik2 is shifted in a positive direction. these changes are consistent with k+ accumulation in the extracellular spaces of the purkinje fibre. 2. these effects of acidosis are completely prevented by increasing [k]0 from the normal range (2.7--5.4 mm) to 10.8 mm. 3. increasing [ca]0 from 2 to 6 mm also produces an inward ...197831464
the effect of nicotine on fetal breathing movements in conscious pregnant ewes.nicotine (0.14--0.25 mg/kg), injected intravenously or intraarterially into conscious pregnant ewes, caused a decrease in fetal pao2 within 5 minutes, persisting for up to 30 minutes. there was a significant fall in the incidence of fetal breathing movements. these changes did not occur if the ewe was treated with an alpha-blocking agent (phentolamine) or if the nicotine was infused for 30 minutes at 0.27 to 0.85 mg/minute. nicotine crossed the placenta; fetal concentrations equaled those in the ...197831587
some physiological effects of ketamine in sheep.the effects of intravenous injections of ketamine in sheep on the cardiovascular and respiratory systems are compared with the effects of intracerebroventricular injections of the same drug and also with the effects of intravenous barbiturate and steriod anaesthetics. intravenously administered ketamine caused an initial fall in arterial blood pressure the extent of which was dose dependent. this depression was short lived and was occasionally followed by a mild pressor phase. intracerebroventri ...197831664
pressure passive cerebral blood flow and breakdown of the blood-brain barrier in experimental fetal asphyxia.cerebral blood flow (cbf) was studied in non-exteriorized near-term sheep fetuses using the radioactive microsphere technique. by partially occluding the umbilical vessels for a period of 1--1 1/2 hours a progressive and severe asphyxia with a final arterial ph of 6.90 was achieved. varying the mean arterial blood pressure in the fetuses by blood withdrawal or infusion in this state, cbf was measured at different perfusion pressures (mean arterial blood pressure (mabp) minus central venous press ...197931759
definitive evidence for renin in rat brain by affinity chromatographic separation from protease.1. angiotensin i-generating activity of rat brain extract was separated into two components by affinity chromatography on a casein-sepharose gel column. 2. the component without affinity to the gel was identified as true renin on the basis of its sensitivity to anti-renin antibody and the lack of protease activity. 3. the second renin-like component with affinity to the gel was a protease insensitive to the anti-renin antibody. its renin-like activity examined with sheep substrate was pronounced ...197832001
transplantation of allogeneic bone marrow from thymus-deprived monkeys and certain characteristics of "graft-versus-host" cells.transplantation of allogeneic bone marrow both from normal and thymus-deprived donors (thymectomized lethally irradiated and restored with autologous bone marrow) was followed 6--7 days later by the appearance of large numbers of gvh cells in bone marrow and peripheral blood which were one of the characteristic features of acute gvhr. gvh cells isolated from peripheral blood 7 days after transplantation showed a high labelling index (about 30%) after 40 min incubation with 3h-thymidine. the prol ...197832079
intestinal rennet activity of newborn lambs. 197832707
trypanosoma (megatrypanum) melophagium in the sheep ked, melophagus ovinus. a scanning electron microscope (sem) study of the parasites and the insect gut wall surfaces.a description of the different stages of trypanosoma (m.) melophagium in different regions of the gut of the sheep ked (melophagus ovinus) as observed by the sem is presented. the extensive pile carpet or palisade colonization of the midgut and pylorus is described. the method of attachment and the relationship of the parasites to the microvilli in the midgut and the cuticle of the pylorus and ileum observed by other methods are confirmed. the micro-structure of the surfaces themselves in the re ...197832751
cardiac and uterine hemodynamic responses to ritodrine hydrochloride administration in pregnant sheep.the changes in the maternal circulation following administration of ritodrine hydrochloride were investigated in chronically prepared pregnant sheep. low infusion rates of ritodrine (see text) elevated the maternal heart rate and cardiac output and decreased peripheral vascular resistance. stroke work fell while minute work increased. the distribution of uterine blood flow did not change, as measured with microspheres. simultaneously measured fetal cardiac output and umbilical blood flow were no ...197932771
intrinsic and extrinsic fluorescence probes of subunit interactions in ovine lutropin. 197832841
cardiovascular reactivity of neonatal and adult sheep to autonomic stimuli during adrenergic depletion.the cardiovascular responses of the neonatal and adult sheep to autonomic agonists and antagonists were studied in the resting state and following adrenergic neuronal depletion with reserpine. both the neonates and adults showed similar supersensitivity of the effector system of the peripheral circulation to alpha adrenergic stimulation with catecholamines. in addition, the neonates exhibited supersensitivity of the myocardial effector system to beta-adrenergic stimulation. unusual finding was a ...197832929
in vivo demonstration of renal carbonic anhydrase activity in the fetal lamb.the capacity of the fetal renal carbonic enzyme to facilitate the renal reabsorption of bicarbonate was studied in 8 long-term fetal lamb preparations (103-124 days of gestation). after acetazolamide administration the fetal arterial blood ph and pco2 values decreased significantly (p less than 0.05). significant increases in fetal urinary ph (p less than 0.001), and urinary excretion of bicarbonate (p less than 0.001), potassium (p less than 0.05) and total cation (na+ + k+) were observed after ...197832932
hemodynamic responses of the sheep fetus to vasopressin infusion. 197932971
[sequence of the changes in the weight percentage of the right heart ventricle in various animal populations under alpine conditions]. 197935906
razdan virus, a new ungrouped bunyavirus isolated from dermacentor marginatus ticks in armenia.a virus, designated razdan, was isolated from dermacentor marginatus ticks in the armenian s.s.r. in 1973. the complement fixation tests revealed no antigenic relationships to 74 tick-borne arboviruses. the size of the virus is about 100 nm; it agglutinates goose erythrocytes at ph 5.5--7.0, is pathogenic for newborn, 14-day-old and adult white mice and multiplies in primary and continuous cell cultures. morphological properties of the virus permit its classification as a member of the family bu ...197835950
dissociation of light chains from cardiac myosin. 197833041
some kinetic and other properties of the isoenzymes of aspartate aminotransferase isolated from sheep liver.a method for the purification of mitochondrial isoenzyme of sheep liver aspartate aminotransferase (ec 2.6.1.1) is described. the final preparation is homogeneous by ultracentrifuge analyses and polyacrylamide-gel electrophoresis and has a high specific activity (182 units/mg). the molecular weight determined by sedimentation equilibrium is 87,100 +/- 680. the amino acid composition is presented; it is similar to that of other mitochondrial isoenzymes, but with a higher content of tyrosine and t ...197935156
the history and present status of aortic surgery in japan, particularly for aortitis syndrome. 197935547
abomasal function following injections of elfazepam and 9-aza-cannabinol.the feed intake stimulants elfazepam (e), a benzodiazepine, and 9-aza-cannabinol (9-ac) decrease rumen contractions and abomasal acid content in sheep and e increases rumen fluid volume, digestibility and overall nutrient availability. e has been hypothesized to decrease the propulsive activity of the entire gi tract. to further examine the effects of e and 9-ac on gastric function, 4 ewes were prepared with abomasal cannulas and 3 silver/silver chloride monopolar electrodes alternated with 2 st ...197935793
eperythrozoon ovis. iii. the effect of infection on blood ph, concentrations of pyruvate, lactate and glucose in blood, haematology, and on concurrent and superimposed trypanosoma vivax infection in sheep.infections of sheep with eperythrozoon ovis has been shown to increase pyruvate and lactate concentrations in blood and to reduce blood ph over a period of 10-14 days post-inoculation. there was no effect on the concentration of blood glucose. however, if care is not taken to inhibit glycolysis, the concentration of blood glucose in samples of blood taken for analysis falls markedly. rapid destruction of erythrocytes started about 10 days post-inoculation and was progressive. superimposition of ...197833476
ecg-changes in the fetal lamb during asphyxia in relation to beta-adrenoceptor stimulation and blockade.progressive changes in the s-t interval of the fetal electrocardiogram (fecg) were studied in 14 lamb fetuses, acutely exteriorized and subjected to graded hypoxia. the aims of the study were to investigate whether beta-adrenoceptor stimulation and hypoxia exerted additive or potentiating effects on the fecg and several cardiovascular parameters and whether the hypoxic changes of the fecg could be blocked by beta-adrenoceptor blocking agents. the fecg changes were studied in order to correlate t ...197933536
[effect of food deprivation on various parameters of rumen juice and blood of sheep].in order to find out how short-time denutrition changes the concentration of some substances in the rumen fluid and the blood, tests with full-grown sheep were carried out. fodder was withheld from sheep with inserted jarrett fistulae for 48 hours after normal feeding. after 48 hours the animals were given concentrated fodder, after 52 hours exclusively hay. from the 72nd hour onwards the animals were provided with fodder as usual. samples of the rumen fluid and blood samples were taken at the b ...197833636
alteration of lymphocyte function in nzb/nzw mice. iv. response to levamisole.nzb/nzw f1 female mice received levamisole in an attempt to increase suppressor t cell function. responses varied with dosage of drug and age of mice. when treatment with 25 microgram/gm doses (intermittent or daily) was begun at 12 weeks of age, nephritis was accelerated in spite of transient reduction of anti-dna antibodies. when treatment was begun at 4 weeks, or when doses of 250 microgram/gm were given, no clinical effects were observed. in the mice with accelerated disease, delayed hyperse ...197933681
mechanism of serine hydroxymethylase catalyzed cleavage of l-erythro-beta-phenylserine: ph dependence of elementary kinetic processes from spectroscopic, pre-steady kinetic, and competitive inhibition studies. 197933702
fallopian tube transplantation and its future [proceedings]. 197833910
fetal and neonatal pulmonary circulation. 197935091
electrogenesis of the plateau and pacemaker potential. 197935092
the role of prostaglandins in the central nervous system. 197935094
physicochemical properties of a proline-rich polypeptide (prp) from ovine colostrum.properties of a proline-rich polypeptide (prp) accompanying ovine colostral igg2 are described. prp is soluble at 4 degrees c but reversibly precipitates by warming to room temperature. maximal precipitation is observed at ph = 4.6, temp. 48 degrees c, and ionic strength higher than 0.6. there is a linear dependence of precipitation on concentration of prp. molecular weight of prp is 38,000 daltons. it is not changed in the presence of 6 m guanidine hydrochloride, sh-compounds, and in the presen ...197835126
[the influence of ritodrine-hcl (prepar), bunitrolol (stresson) and oxytocin (syntocinon) on uteroplacental perfusion (author's transl)].the administration of betamimetics has proved to be useful for symptom-oriented treatment in patients with reduced utero-placental flow. in order to quantify the betamimetic effect flow measurements were carried out in a total of 12 sheep, yielding the following experimental results: 1) betamimetics, up to a certain point which correlates with maternal heart rate, raise the uteroplacental flow-rate. 2) the use of cardio-selective betablocker prevents undesired betamimetic side-effects but there ...197935885
alpha-glucosidase activity in the reproductive tract of the ewe.alpha-glucosidase activity has been estimated in the tissues and rinsings of the reproductive tract of the ewe. there were peaks of activity in the oviducal mucosa at ph 4.0 and 5.7. in the endometrium, caruncles and cervical mucosa and ph optimum occurred from ph 4.0 to ph 5.7. a sharp peak in the activity in the vaginal mucosa occurred at ph 5.7. the only tissue in which changes in enzyme activity were consistently related to one endocrinological state of the ewe was the cervical mucosa. cervi ...197836061
regional cerebral blood flow changes during severe fetal asphyxia produced by slow partial umbilical cord compression.we studied the effects of severe partial asphyxia on regional cerebral blood flow and arterial blood pressure in the unanesthetized, physiologically stable fetal lamb. cerebral blood flow was measured by the microsphere technique before and during partial umbilical cord compression. asphyxia sufficient to decrease ph from 7.40 to 7.04 and reduce oxygen saturation from 50% to 19% increased cerebral blood flow to all areas of the brain with the largest increases going to the brain stem (275% of co ...197938666
the maintenance of motility and the surface properties of epididymal spermatozoa from bull, rabbit and ram in homologous seminal and epididymal plasma.epididymal spermatozoa from bull, rabbit and ram were incubated in homologous epididymal plasma or seminal plasma in a buffered saline-based medium with or without serum albumin. the spermatozoa were either diluted directly into the medium or were washed first. no effect of washing was observed on the subsequent reaction of the cells to the different media. a considerable proportion of the populations of epididymal spermatozoa survived (i.e. continued to exhibit motility) for up to 22 h at 30 de ...197934036
digestion, absorption and transport of lipids in ruminant animals. 197834168
[5th international symposium on infectious diseases in sheep and goats. tunis, 20-22 november 1978]. 197934245
trimethoprim in sheep influence of ph and protein binding on mammary and renal excretion. 197934297
the role of the colostrum in relation to immunity and survival in the newborn ruminant and pig. a seminar held in the institut national agronomique de paris-grignon, 78850 thiverval-grignon, france. april 25, 26 and 27th 1978. 197834350
the behaviour of thiomolybdates in in vitro systems.the stability of potassium tetrathiomolybdate was studied in vitro using solutions with molybdenum, hydrogen ion and phosphate concentrations similar to those normally found in the rumen. under these conditions k2[mos4] hydrolysed rapidly and as a result the solution contained [mos4]2-, [moos3]2-, [moo2s2]2-, [hs]- and h2s in equilibrium. in view of this hydrolysis, in vivo studies of thiomolybdate on copper metabolism of sheep should not exclude the possibility that either sulphide or molybdate ...197934421
preparative flatbed electrofocusing in granulated gels with natural ph gradients generated from simple buffers. 197939470
neurogenic dilator and constrictor responses of pial arteries in vitro. differences between dogs and sheep. 197934490
sex differences in fetal sheep adrenal steroidogenesis.the formation of several steroids was determined in vitro in adrenals removed from 18 female and six male fetuses of 113-115 days' gestation and in two female and two male fetuses at near term (137-143 days). samples were incubated with 14c-acetate and the formation of labeled steroids was determined by two-dimensional paper chromatography. protein and corticosterone concentrations were determined by chromophore absorption and acid hydrolysis fluorescence methods, respectively. tissue corticoste ...197839835
fetal breathing: a review.the history, physiology, pharmacology, and clinical application of fetal breathing are reviewed. early optimism that measurements of fetal breathing would aid in perinatal management decisions has remained unfulfilled. problems to be overcome include standardization of technique and measurement parameters and allowance for physiologic variations. recent research developments indicate that the likely role of fetal breathing in perinatal medicine will be in conjunction with other established tests ...197936761
[local infection treatment in accident surgery. c. gentamycin-containing pmma-spheres and chains. experimental principles for the application of antibiotic-containing polymethylmethacrylate]. 197936768
dopamine treatment of spinal hypotension decreases uterine blood flow in the pregnant ewe.in seven pregnant ewes, 3--5 min of hypotension resulting from spinal anesthesia decreased uterine blood flow 17 per cent. dopamine, in doses sufficient to maintain blood pressure at control values (20--40 micrgram/kg/min) for 3--5 min, further decreased uterine blood flow to 56 per cent less than control and increased uterine vascular resistance to 50 per cent more than control. following 30 min of hypotension, 30 min of dopamine administration in doses sufficient to restore blood pressure to c ...197936821
effects of changes in maternal--fetal ph on the transplacental equilibrium of bupivacaine.increases in the maternal-fetal ph gradient that may occur during labor and delivery may increase the fetal concentration of local anesthetics. the authors evaluated effects of ph changes on the transplacental concentration equilibrium of bupivacaine. they increased the maternal-fetal ph gradient in each of six pregnant ewes from a control value of 0.15 to 0.54 by hyperventilating the lungs of the ewe and infusing lactic acid into her fetus. after infusion of bupivacaine, 0.15 mg/kg, intravenous ...197936822
characterization of prolactin binding by membrane preparations from rat liver.binding sites for prolactin were identified in a plasma-membrane-enriched fraction isolated from livers of mature female rats. 125i-labelled sheep prolactin prepared by the lactoperoxidase procedure retained the same molecular integrity and binding affinity as the native hormone at physiological ph. the receptors bound prolactin from different species, whereas non-lactogenic hormones were not bound. the binding of 125i-labelled sheep prolactin was activated equally by bivalent and univalent cati ...197936884
the effects of nitrilotriacetic acid on solubilities of zinc, copper, manganese, and iron in the stomach of sheep.four sheep, each prepared with a rumen fistula and reetrant cannula in the proximal duodenum, were used to study the effects of ruminal administration of nitrilotriacetic acid on solubilities of zinc, copper, manganese, and iron in rumen and duodenal digesta. the sheep received a pelleted diet and were dosed with 0, 300, 600 and 1200 microgram of nitrilotriacetic acid per gram of diet via the rumen fistula. higher concentration of soluble zinc, manganese, and iron but not copper, were found in t ...197936975
effects of low electrolyte media on salt loss and hemolysis of mammalian red blood cells.cation loss and hemolysis of various mammalian red cells suspended in isotonic non-electrolyte media were investigated. sucrose buffered with 10 mm tris-hepes, ph 7.4 was used as the non-permeable non-electrolyte. mammals from which the red cells were derived include the human, guinea pig, rat, rabbit, newborn calf, newborn piglet and pig, all of which contain k as the predominant cation species (hk type) and the dog, cat, sheep and cow, all of which possess na as the predominant cation species ...197939943
divergent results in radial immunodiffusion. iii. isolation and specificity test of class-specific antibodies.a method is described for the isolation of antibodies specific for immunoglobulin class determinants from antisera containing antibodies both for the immunoglobulin class and for its subclasses, using affinity chromatography. the model system used is bovine igg with its subclasses igg1 and igg2, together with antisera raised in goats and sheep. the specificity of the isolated class-specific antibodies was demonstrated by double diffusion (di) and radial immunodiffusion (rid). the levels of total ...197937277
sheep fetal haematopoietic cells produce adult haemoglobin when transplanted in the adult animal. 197937447
distribution of glutamate dehydrogenase and glutamine synthetase activity in the sheep and chicken digestive tract.glutamate dehydrogenase (gldh, ec 1.4.1.3) and glutamine synthetase (gs, ec 6.3.1.2) activity were determined in the contents and tissues of the various parts of the sheep and chicken digestive tract, gldh activity in the tissues of the sheep omasum, duodenum, rumen, reticulum, colon, caecum, jejunum and ileum ranged from 3.25+/-0.7 u (mumol/g dry weight . min) to 5.94+/-2.28 u; in the abomasum it was 9.67+/-1.27 u. gldh activity in the contents of the ileum, abomasum, jejunum and duodenum varie ...197937534
international committee communications. report from the subcommittee on heparin. 197937611
lymphocyte traffic within the bone marrow and selective retention of alloreactive cells.earlier studies showed that large numbers of isotopically labelled thoracic duct lymphocytes (tdls) enter the bone marrow (bm) within hours of injection but depart equally as rapidly by 12 to 24 hr. the significance of this rapid flux was investigated further. early (1/2 to 2 hr) after the i.v. injection of tdls, bm was shown to contain t cells capable of initiating a graft-versus-host (gvh) reaction in f1 hybrids and in other experiments memory cells against human serum albumin (hsa). both gvh ...197937619
[water intake, ph and ammonia levels in the ruminal contents of sheep fed pelleted feed].wethers were studied for the effect of the complete pelleted feed ration on the intake of water and concentration of ph and ammonia in the rumen. the animals consumed daily 1300 g of dry matter of the diet containing 41.81% of meadow hay, 25.8% of barley, 15.37% of sawdust, 14.98% of molasses, 1.32% of urea and 1.24% of vitamin mineral supplement. this feed was given to them for six months. the pelleted diet increased the intake of water (the dry matter consumption remaining the same in the cont ...197937631
in vitro binding of trypanosoma congolense to erythrocytes.trypanosoma congolense broden, an intravascular parasite, binds to vessel walls and erythrocytes of infected hosts. in an attempt to characterize t. congolense adhesion to host cells, an in vitro assay was devised. it was shown in the in vitro experiments that t. congolense binds to bovine, sheep, and goat erythrocytes, but not always to erythrocytes of rats, mice, rabbits, horses or humans. only the anterior part of live trypanosomes adheres to erythrocytes, and the attachment site on the trypa ...197939167
polyarteritis nodosa associated with sarcocystosis in a lamb. 197939432
[dynamics of acid-base indices in the blood of lambs from the 7th day of postnatal life to adult age].under the conditions of a specialized farm equipped with amodern technology or rearing, sixteen ewe lambs of the improved wallachian breed were studied for the dynamics of acid-base indices in the blood from the seventh day of post-natal life unitl the age of 19 months. the acid-base parameters were measured by the astrup bms 2 apparatus. after weaning at an age of 45 days, the lambs were fed mixture coj 1 for ten days, then mixture coj 2, followed by the administration of a vj mixture and granu ...197937632
effect of synthetic prostaglandin pgf2 alpha on the volume, acidity, and proteolytic activity of the abomasum juice in sheep.effect of synthetic prostaglandin pgf2 alpha on the volume acidity, and proteolytic activity of the abomasum juice in sheep. act physiol. pol., 1979, 30 (2): 299--304. the investigations were carried out in 3 male sheep on the effect of estrumate (a synthetic analogue of pgf2 alpha) on the secretion and composition of juice from the "small" abomasum. after intramuscular injection of optimum doses of the preparation (3.0--4.0 micrograms/kg of body weight) it was observed that the volume of the ju ...197937695
binding of coenzyme and substrate and coenzyme analogues to 6-phosphogluconate dehydrogenase from sheep liver. an x-ray study at 0.6 nm resolution.the analogues of the coenzyme nadp+, nicotinamide--8-bromo-adenine dinucleotide phosphate (nbr8adp+) and 3-iodopyridine--adenine dinucleotide phosphate (io3pdadp+), were prepared. nbr8adp+ was found to be active in the hydrogen transfer adn io3pdadp+ is a coenzyme competitive inhibitor for 6-phosphogluconate dehydrogenase. the binding of nadp+, nadph and nadph together with 6-phosphogluconate as well as that of both analogues to crystals of the enzyme 6-phosphogluconate dehydrogenase has been in ...197938116
the development of the binocular depth cells in the secondary visual cortex of the lamb.in most respects, the response properties of cells in the secondary visual cortex of the newborn lamb were indistinguishable from those in the adult. the cells were sharply selective to orientation; the orientation preferences were the same in each eye, and they varied systematically as the electrode penetrated the cortex. the receptive-field organization did not differ noticeably from that in adults, and complex, hypercomplex, and a few simple cells were all observed. the ocular dominance distr ...197938458
lipid metabolism in the mammary gland of ruminant animals. 197938463
effect of membrane potential and internal ph on active sodium-potassium transport and on atp content in high-potassium sheep erythrocytes.ouabain-sensitive na+ and k+ fluxes and atp content were determined in high potassium sheep erythrocytes at different values of membrane potential and internal ph. membrane potential was adjusted by suspending erythrocytes in media containing different concentrations of mgcl2 and sucrose. concomitantly either the external ph was changed sufficiently to maintain a constant internal ph or the external ph was kept constant with a resultant change of internal ph. the erythrocytes were preincubated b ...197938843
arginine vasopressin during gestation and parturition in sheep fetus.the possible correlation between plasma arginine vasopressin (avp) concentration and the processes leading to parturition was assessed in 11 chronically catheterized pregnant ewes. samples of blood withdrawn intermittently during a 20-day period preceding labor and during parturition were analyzed for avp by a specific radioimmunoassay, as well as for ph, paco2 and pao2. fetal avp was 1.74 +/- 1.55 pg/ml and maternal avp 1.47 +/- 0.74 pg/ml (mean +/- sd). no preparturient rise in fetal vasopress ...197938861
certain aspects of hemorheology in a near zero gravity environment. 197938870
adherence of erythrocytes to mycoplasma pneumoniae.the human pathogen mycoplasma pneumoniae adheres to a variety of cells, including erythrocytes. a hemadsorption technique was developed to quantitate adherence by photometric measurement of lysates of erythrocytes that attached to sheets of m. pneumoniae grown in cups of linbro plates. attachment of sheep erythrocytes (se) increased with higher ionic strength, was unaffected by minor ph variations (6 to 9), and was blocked by anti-m. pneumoniae antiserum, but was not inhibited by a variety of su ...197939034
fetal respiration: a review of two techniques for observation. 197939701
placental clearance of lactate and bicarbonate in sheep.to determine the placental clearance of lactate and bicarbonate in sheep, the fetal side of an isolated cotyledon and the umbilical circulation of the total placenta were artificially perfused. the release and uptake of lactate and bicarbonate by the perfusion fluid and the fetomaternal concentration differences of these substances were measured. from these data, the clearance of lactate and bicarbonate was determined to be 0.9 (se = 0.2) ml/h/g of placental tissue. the production of lactate by ...197939880
effects of hydrocortisone on binding of igg or c3b-coated erythrocytes to human monocytes and polymorphonuclear leucocytes. 197940004
1,10-phenanthroline mediated hemolysis--evidence that 1,10-phenanthroline does not substitute specifically for c9. 197940111
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