| binding specificity of rabbit anti-guinea pig epidermal cell sera: comparison of their receptors with those of concanavalin a and pemphigus sera. | rabbit anti-guinea pig epidermal cell sera (aes) which were prepared by immunizing rabbits with enzymatically dispersed viable guinea pig epidermal cells were shown to react to the intercellular substances of stratified squamous epithelia of guinea pigs and monkeys in a pattern similar to that seen with concanavalin a (cona) or pemphigus sera (ps) by immunofluorescence. reciprocal blocking tests were performed on guinea pig lip mucosa after removal of the non-specific binding substances between ... | 1979 | 84486 |
| [distribution of orally administered aflatoxin b 1 in the tissues and organs of the goat (capra)]. | in the experiment the goat was administered an amount of 450 mg aflatoxin b1. the milk taken during the experiment was lyophilized and aflatoxins b1 and m1 were isolated. after the death of the goat some tissues, blood and bile of the experimental animal were analyzed to find out the aflatoxin content. | 1978 | 100931 |
| protein a reactivity of various mammalian immunoglobulins. | serum samples and immunoglobulin fractions of eight mammalian species were applied to a sepharose--protein a column. as with the human immunoglobulin subclasses igg1, igg2 and igg4, all examined igg classes and subclasses were bound to a greater or lesser extent to protein a. however, the binding of igg1 of ruminants was very poor. polyclonal igm and iga of the pig, the dog and the cat may be separated in protein a reactive and protein a non-reactive fractions. in addition, monoclonal canine igm ... | 1978 | 100867 |
| tissue distribution of human alpha1-microglobulin. | human alpha(1)-microglobulin was isolated from the urine of patients with tubular proteinuria, and its molecular weight was established by sodium dodecyl sulfate-polyacrylamide gel electrophoresis at 33,000 daltons. the carbohydrate content was 21.7%. anti-alpha(1)-microglobulin serum was prepared and observed to react monospecifically in gel diffusion to purified alpha(1)-microglobulin, as well as to normal human serum and urine. sera from the domestic chicken, mouse, rat, rabbit, dog, calf, co ... | 1979 | 85635 |
| amyloid p component--a special type of collagen? | the localization of amyloid p-components is demonstrated by immunofluorescence microscopy in normal human tissue (kidney, spleen, liver). the relation to collagen and to amyloidosis is discussed. | 1978 | 104431 |
| effects on reticulo-rumen motility and body temperature of e. coli endotoxin on injection into the medulla oblongata and third ventricle of small ruminants. | | 1978 | 104506 |
| species-dependent stereospecific serum protein binding of the oral anticoagulant drug phenprocoumon. | 13 mammalian species are classified into 3 clearcut groups with respect to the stereospecific serum protein-binding of phenprocoumon: 2 groups showing opposed stereospecific binding characteristics and a 3rd group exhibiting no stereospecific binding. structural differences in the albumin molecule account for these stereospecific differences in serum protein-binding. | 1978 | 104885 |
| sarcocystis sp from the goat in india. | | 1978 | 105455 |
| [origin and biochemistry of rumen acidosis. 6. renal excretion of lactic acid isomers]. | | 1978 | 105509 |
| haematological studies on domestic animals in nigeria i. factors influencing the haematocrit of sheep and goats. | | 1978 | 105514 |
| studies on species variation in the lipid composition of milk fat globule membranes and mammary cell plasma membranes. | | 1978 | 105517 |
| brucella cultures typed by the who brucellosis centre at the commonwealth serum laboratories, melbourne. | results of the typing of brucella cultures received at the who brucellosis centre, csl, melbourne, from 1968-1976 are presented. the distribution of the biotypes of cultures recovered throughout australia is shown on a host and state basis and atypical cultures are discussed. cultures identified from australia were br. abortus, biotypes 1, 2 and 4 and strain 19; br. suis, biotype 1 and br. ovis. br. melitensis biotypes 1, 2 and 3 were recorded only as exotic human infections from the mediterrane ... | 1978 | 105700 |
| immunochemical, serologic, and immunologic properties of major antigens isolated from the legionnaires' disease bacterium. observations bearing on the feasibility of a vaccine. | two antigens were isolated from each of three strains of the legionnaires' disease (ld) bacterium. one antigen was serotype-specific; the other cross-reacted with strains of ld bacteria of different serotypes. the serotypic antigens contained all the major branched-chain fatty acids characteristic of ld bacteria and were a lipid-protein-carbohydrate complex. electrophoresis resolved the serotypic antigen of knoxville 1 strain into four protein bands and one glycoprotein band with molecular weigh ... | 1979 | 86314 |
| binding properties of goat igm anti-dinitrophenyl antibodies. | goats immunized over 2 months with low doses of 1 mg/kg of dinitrophenylated salmonella typhimurium responded with low levels of anti-dnp antibodies restricted to the igm class. the purified antibodies show low association constants (ka between 10(4) of 10(5) l/m), a high degree of homogeneity (heterogeneity indices alpha between 0.7 and 0.9) and ten combining sites when tested against dinitrophenyl-lysine as ligand by equilibrium dialysis. these binding properties remained unchanged during the ... | 1978 | 86504 |
| a partial characterization of hepatitis b e antigen. | partially purified hepatitis b e antigen (hbeag) was prepared by ultracentrifugation, ammonium sulfate precipitation, and molecular sieve chromatography of sera obtained from asymptomatic carriers of hepatitis b surface antigen. the antigenic specificity of the hbeag preparations was investigated further with affinity chromatography. the results indicated that hbeag is distinct and separable from dna polymerase activity. columns coupled with either goat igg prepared from antiserum to human igg o ... | 1979 | 86589 |
| human leukaemia-associated antigens expressed by acute lymphocytic leukaemias and their detection with heterologous antisera to t, b-, and non-t-non-b subtype al blasts. | antisera from rabbits and goats against subtypes of acute lymphocytic leukaemia (all with t-cell markers, all with b-cell markers, non-t-non-b all) were tested for their specificity in complement-dependent in-vitro cytotoxicity testing. after absorption of the fivefold diluted antisera with erythrocytes and spleen cells of allogenous donors they reacted with all cells, but not with leukaemias of other types (aml, cll, cml), lymphocytes of healthy donors, enriched b-lymphocytes, enriched t-lympho ... | 1979 | 89983 |
| specificities of antibodies to acetylcholine receptors. | antisera to acetylcholine receptor purified from the electric organs of torpedo and electrophorus were highly species specific, but showed limited cross-reaction with receptors from all mammalian species tested. antisera to receptor purified from rat muscle cross-reacted extensively with receptor from the muscles of other mammals. antibodies to receptor from electrophorus cross-reacting with receptor from torpedo were affinity purified. subsequently these showed increased cross-reaction with rec ... | 1978 | 86951 |
| evidence for the location of high mobility group protein t in the internucleosomal linker regions of trout testis chromatin. | antibodies against the trout testis non"histone chromosomal protein, high mobility group protein t (hmg-t), have been elicited in goats. the antiserum was shown to be specific for hmg-t and did not cross-react with histone 1 or with the other two trout testis hmg proteins, h6 and ubiquitin. purified anti-hmg-t igg was used to determine the location of hmg-t within chromatin subunits separated on sucrose gradients. binding of fluorescent labeled anti-hmg-t to these subunits clearly supports the n ... | 1979 | 107172 |
| immunologic relationship between rinderpest and peste des petits ruminants viruses. | | 1975 | 86993 |
| activation of the alternative pathway of complement by antiserum to factor b. | monospecific rabbit and goat antisera to human complement proteins and human immunoglobulins were tested for their ability to activate the alternative complement pathway. this activation was detected by two methods where classical pathway activation was blocked with egta and alternative pathway activation was promoted with added magnesium ions. these two methods consisted of lysis of gshe and conversion of factor b into split products. c1q-depleted serum was used in a third assay system. only an ... | 1979 | 107255 |
| isolation of listeria monocytogenes from animals in the sudan. | | 1979 | 107996 |
| properties of a goat anti-l antibody: further evidence for heterogeneity of the l antigen. | the preparation and properties of an antibody (anti-l) against low potassium type (lk) goat red cells raised in a high potassium type (hk) goat are described. this reagent stimulated active potassium transport, but showed only weak serological activity against low potassium type (lk) sheep and goat red cells. the results are discussed in relation to the hypothesis that anti-l antibody has two specificities--a sodium pump-stimulating activity (anti-lp) and a serological activity (anti-l1y). | 1979 | 87226 |
| avian antigen-binding cells. i. characterization of antigen-binding t cells from bursectiomized chickens by autoradiography. | antigen-binding cells (abc) from spleens of hgg-immunized, bursectomized agammaglobulinemic (bx) chickens were detected by direct autoradiography with 125i-hgg and by sandwich autoradiography with hgg plus 125i-goat-anti-hgg. the specificity of antigen binding was demonstrated by 1) inhibition of binding of 125i-hgg by preincubation with unlabeled hgg and 2) a specific increase in abc after immunization. the abc from bx chickens were not b cells, as shown by the virtual absence of immunoglobulin ... | 1979 | 90701 |
| [an in-vitro method of ruminal juice cultivation, suitable for the comparison of various sources of nonprotein nitrogen for ruminants]. | the method of cultivating rumen fluid in buffer with nutrient admixture under co2 atmosphere is described. the method serves for the comparison and description of the properties of npn sources for ruminants. its applicability is demonstrated on the example of urea. during incubation, all parameters of the medium remain within an admissible range. considering the analyses of the incubation medium, i. e. determination of the ph value, ammonia, urea, volatile fatty acids, total protein, and redox p ... | 1979 | 108840 |
| [genesis and biochemistry of rumen acidosis. 7. oxydation rate and quantitative gluconeogenesis from d-lactate-(14c) in goats]. | | 1979 | 108879 |
| comparative morphology and mucus histochemistry of the ruminant cervix: differences between crypt and surface epithelium. | | 1979 | 91389 |
| metabolism of l-[u-14c; 2,3-3h] valine in perfused goat mammary glands [proceedings]. | | 1979 | 92967 |
| fractionation of antibodies to l-cell colony-stimulating factor by affinity chromatography. | purified l-cell colony-stimulating factor (csf) was coupled to cyanogen-bromide-activated sepharose and used to selectively fractionate antibodies to this factor. with the use of a simplified two-step washing and elution technique, there was 50%--70% binding of the anti-csf, with recovery of 60%--100% of the bound material. both the native antiserum and purified anti-csf fractions were inhibitory to murine granulocyte-macrophage colony formation. the purified antibodies contained only igg and w ... | 1979 | 109132 |
| sarcocystis in goats: prevalence and transmission. | | 1978 | 109801 |
| are vh framework antigenic determinants expressed on both rabbit b and t lymphocytes? | | 1979 | 93052 |
| cross-reactions between serum proteins and water soluble liver tissue antigens of the nine-banded armadillo (dasypus novemcinctus linn.) and man. | cross-reactions between serum proteins and water soluble liver antigens of the nine-banded armadillo (dasypus novemcinctus linn.) and man were studied by crossed immunoelectrophoresis (cie). armadillo serum tested with rabbit antiserum against human serum proteins gave twelve components in cie. nine of these cross-reacting proteins were identified and showed partial identity with the corresponding human proteins. the electrophoretic mobility of alpha 2-macroglobulin and gc-globulin differed in t ... | 1979 | 93527 |
| a comparative study of serum phospholipase a2 in man & animals--some biochemical & physiological implications. | | 1979 | 112047 |
| [epidemiologic and serologic study of listeriosis in man and domestic and wild animals in austria]. | in the framework of a study aimed to ascertain the possibilities of infection transmission from mother to child the athors examined serologically gravid women for toxoplasmosis and antibodies against listeriosis, using routine methods, in austria in 1974, 1975 and 1975. almost all gynecologists of the region of styria (austrial), who systematically delivered data concerning abortions, premature births and stillbirths, were invited to take part in the study. at the same time data concerning domes ... | 1978 | 112179 |
| [listeriasis in goats]. | | 1979 | 112711 |
| a study of leptospirosis among animals in barbados w.i. | microscopic agglutination tests performed during 1971 and 1972 on barbadian livestock showed positive serological reactions for leptospirosis as follows: cattle 51%, pigs 13%, sheep 18%, horses 64% and goats 19%. the serogroup autumnalis predominated in all but horses in which the reactions to the pyrogenes group appeared slightly higher. after the isolation of a strain of leptospira serotype fort-bragg (barbadian strain no. 119) of the autumnalis serogroup in 1973, and its subsequent inclusion ... | 1979 | 112729 |
| incomplete inhibition of the immunoglobulin g-binding behavior of nitrated protein a. | nitration of protein a with tetranitromethane incompletely blocks its fc-binding ability for immunoglobulin g when measured by more sensitive methods of immunoprecipitation and when tested in the enzyme-linked immunosorbent assay. in addition, binding of protein a and blockage by nitration is heterogeneous and depends on the species origin of the immunoglobulin g. | 1978 | 99370 |
| a simple method for quantifying igm of low molecular weight. | a new and simple method for quantitation of igm of low molecular weight in sera by radial immunodiffusion in 7% agar gel is described. the test is easy to perform and permits exact determination of low molecular weight igm concentration down to very small quantities (1 mg%). results obtained with the method described agree with previously used assay procedures and offer the advantage of convenience and rapidity. | 1978 | 102700 |
| pharmacology of antiarthritic drugs. | | 1978 | 103272 |
| potency testing of anthrax spore vaccine (living) in guinea pigs. | | 1979 | 113339 |
| rauscher leukemia virus-induced tumor antigens: complete separation from gp70, p30 and h-2. | the possibility that some or all of the viral proteins, gp70, p30, and the histocompatibility antigen, h-2, function as the tumor-specific transplantation antigen (tsta) of the r-mulv-induced leukemia, rbl-5, and also in the secondary in vitro induction of cytotoxic t lymphocytes (ctl), was investigated. the antigen was obtained by isolating the plasma membranes of rbl-5 cells and solubilizing with sodium deoxycholate (doc) followed by gel filtration chromatography. a fraction containing excelle ... | 1979 | 93583 |
| [comparative study of various chlamydia strains--preliminary study (author's transl)]. | | 1979 | 94564 |
| studies on the fc gamma receptor of the murine macrophage-like cell line p388d1. i. the binding of homologous and heterologous immunoglobulin g1. | | 1979 | 114580 |
| endocrine control of parturition. | | 1979 | 115023 |
| transplantation of the testis. | | 1979 | 115123 |
| clearance and glomerular localization of preformed dnp anti-dnp immune complexes. | the in vivo behaviour of well-defined immune complexes in rats was studied using complexes derived from dnp-conjugated bovine thyroglobulin (dnp-btg) and purified specific goat anti-dnp igg. both clearance and glomerular localization were mainly dependent on the nature of the antigen. soluble immune complexes formed with dnp17-btg were cleared faster and showed a more marked localization in the glomerular mesangium than complexes formed with dnp3.4-btg. a slight increase in the antibody to antig ... | 1979 | 94694 |
| cross-reactivity of rat, mouse, and human igd. | highly purified sheep anti-rat lymphocyte membrane igd (migd) was used to detect cross-reactivity with the putative murine-delta chain on mouse lymphocytes. cross-reactivity is demonstrated by indirect immunofluorescent staining and by immunoprecipitation of 125i-labeled lymphocyte membrane extracts followed by electrophoresis on 10% polyacrylamide gels. in addition, cross-reactivity of anti-rat-delta with human igd is shown by gel diffusion analysis. the anti-rat-delta reagent stained both ig5a ... | 1979 | 115921 |
| dnases in milk and blood sera from different species. | | 1979 | 116487 |
| paper electrophoretic studies on water soluble proteins of various animal lenses. | | 1979 | 116961 |
| antigenic homology of feline and human beta-hexosaminidase. | the immunological characteristics of feline beta-hexosaminidase (beta-d-n-acetylglucosaminidase, ec 3.2.1.30) isoenzymes, hex a and hex b, were studied. immunization of rabbits and goats with either cat hex a or hex b produced antibodies which reacted with a common antigenic marker shared by both hex a and hex b. with properly absorbed antisera, a unique antigenic marker was demonstrated on cat hex a, but not on hex b. this antigenic profile is comparable to that of the human beta-hexosaminidase ... | 1979 | 94833 |
| [the heterogeneity of streptokinases]. | purified streptokinases of some strains of different types of strep. pyrogenes (group a) as well as streptokinases of a strain of group c or group g streptococci, resp., were studied in regard of their heterogeneity. differences of the streptokinases were found in their amino acid compositions and in isoelectric points. serological differences were detected by a quantitative precipitation reaction with fluorescein-coupled antistreptokinase antisera from the goat as well as by neutralization reac ... | 1979 | 94885 |
| biochemical and serological characteristics of reverse transcriptase from human spleen in a case of childhood myelofibrotic syndrome. | | 1977 | 95799 |
| [confirmation of the presence of uteroglobin in tubal secretions]. | the acrylamide gel electrophoresis of rabbit serum, of rabbit tubal secretions at the oestrus stage (sto) and at the luteal stage (stl), followed by immunodiffusion with anti-serum, was done to detect any tubal proteins which were not found in the rabbit serum. the anti-rabbit sto goat anti-serum was absorbed with rabbit plasma in order to purify antibodies to proteins found specifically in tubal secretions. this technique revealed the presence of a protein (stx) in sto and stl but not in the ra ... | 1979 | 117524 |
| leukocytic endogenous mediator. | | 1978 | 97380 |
| isolation of immunoglobulin from splenic lymphocytes of inbred rats by triton x-100 solubilization. | | 1978 | 98589 |
| studies on purification and characterization of sheep testicular inhibin. | | 1979 | 118253 |
| an enzyme linked immunosorbent assay (elisa) for detecting igg sensitized erythrocytes. | this paper describes an enzyme linked immunosorbent assay (elisa) for detecting igg sensitized erythrocytes utilizing a commercially available anti-human igg conjugated with alkaline phosphatase. erythrocyte hemolysis in the assay was minimized by dissolving the p-nitrophenyl phosphate substrate in a carbonate-bicarbonate buffer. nonspecific absorption of the enzyme conjugate to erythrocytes and glassware was reduced by adding 1% bovine serum albumin to wash solutions. assay sensitivity was incr ... | 1979 | 118558 |
| partial characterization of xanthine oxidase (xo) from buffalo milk fat globules and variation in the xo content of milk. | | 1979 | 119386 |
| [fluorescent serological differentiation of species of the genus brucella]. | | 1979 | 119388 |
| immunoglobulin-specific radioimmunoprecipitation assays for quantitation of nasal secretory antibodies to hemagglutinin of type a influenza viruses. | radioimmunoprecipitation (rip) assays were developed to selectively quantitate class-specific antibodies to purified hemagglutinins (ha) of type a influenza virus in nasal secretions. rabbit anti-human secretory piece of immunoglobulin a (iga) and rabbit anti-human igg were used as second antibodies. a third antibody, goat anti-rabbit igg, was incorporated into the system to separate immune complexes formed between iodinated ha, nasal wash test specimen, and second antibody. the utilization of t ... | 1978 | 100506 |
| characterization of antigens from aspergillus fumigatus. ii. fractionation and electrophoretic, immunologic, and biologic activity. | mycelial extracts of aspergillus fumigatus grown in completely synthetic medium were fractionated by precipitation with ammonium sulfate, by ion-exchange chromatography, and by gel filtration. the fractions were characterized chemically, by analytic polyacrylamide gel electrophoresis, and by tests for serologic and biologic activity. the distribution of the antigens in the fractions was determined by double diffusion in gel and fused-rocket immunoelectrophoresis. activity greater than that of th ... | 1978 | 101104 |
| a single-leaflet mitral valve: its design, development and evaluation in long-term animal trials. | | 1978 | 102061 |
| absorption test using latex particles as the indicator system for the species identification of bloodstains and muscles. | this paper reported the species identification of bloodstains and muscles by means of an absorption test in which antisera were absorbed by bloodstains or muscles and then the absorbed antibody capacity was titrated by using latex particles coated with corresponding serum proteins. this test method was proved to be simple, specific, and sensitive. | 1979 | 120405 |
| [differentiation of nonspecific serological reactions in brucellosis]. | differentiation of non-specific agglutination was performed by the complement binding reaction, coombs' reaction, hajdu reaction, the surface fixation and agglutination reaction and the reaction of complement binding with heterologic antigens. for that purpose the following were used: 1) serums--antiglobulin against cattle globulin, 5720 serum of various animals which had manifested non-specific agglutination with brucella antigen and brucella serums of experimentally infected sheep, of naturall ... | 1979 | 120633 |
| immunohistochemical demonstration of carbonic anhydrase. | rabbits were immunized using human erythroxyte carbonic anhydrase b (hca b) purified by the modified methods of armstrong et al. (1966) and bernstein and schraer (1972). the globulin fraction was isolated by ammonium sulphate precipitation. the anti-hca b globulin was specific, when judged using the double diffusion technique of ouchterlony and immunoelectrophoresis. no cross reaction with human erythrocyte carbonic anhydrase c was found, but cross reactions with erythrocyte carbonic anhydrase f ... | 1978 | 103868 |
| a comparative study for component amino acids in soluble & insoluble protein fractions of eye lenses of some mammals. | | 1979 | 120846 |
| gravity, metabolic rate and body size of mammals. | | 1979 | 120950 |
| the composition and calcium transport activity of the sarcoplasmic reticulum from goats with and without heritable myotonia. | studies were conducted on purified sarcoplasmic reticulum isolated from myotonic goats, an animal model of heritable myotonia. when compared to sarcoplasmic reticulum from normal goats, fragmented sarcoplasmic reticulum from the myotonic goat had (1) increased levels of calcium, (2) increased rates of calcium uptake and efflux, (3) an increased sialic acid content, and (4) an increased content of saturated fatty acids. these differences support the concept of a structural and functional defect a ... | 1979 | 106184 |
| physicochemical and comparative studies on bile proteins. | | 1979 | 121295 |
| purification and characterization of lecithin:cholesterol acyltransferase. | the purification of lecithin:cholesterol acyltransferase (lcat] from human plasma is reported. hydroxylapatite fractions were approximately 16,000 fold purified over the starting plasma and were free of high-density lipoprotein (hdl) and albumin. the enzyme showed one band on polyacrylamide gel electrophoresis, sds-urea polyacrylamide gel electrophoresis, isoelectric focusing, and one arc in immunodiffusion against a goat antiserum preparation. it was determined to be a glycoprotein with a molec ... | 1978 | 106459 |
| immunological evaluation of lcat deficiency. | antibody towards a highly purified lcat preparation was tested against lcat-deficient sera by using the technic of immunodiffusion and immunoinhibition. reaction of identity among normal serum, deficient sera, and purified lcat was observed in immunodiffusion with two different antibodies obtained from two different goats. when the antibodies were mixed with deficient sera and tested for immunoinhibition of lcat activity, suggesting that deficient sera contained an enzymatically inactive lcat. t ... | 1978 | 106460 |
| human thrombin autoantibodies. | | 1978 | 106485 |
| the change induced by anti-l in the sodium and potassium affinities of the sodium pump in lk erythrocytes. | | 1975 | 124772 |
| development of skin interfacing cannula. | the skin interfacing potential of various velours, felts, foams and rough cast surfaces of some polymers was investigated by bonding these substances to solid core silastic rods using dow-corning medical adhesive type a. these dummy cannulae were implanted as skin penetrating rods onto the dorsum of canines, goats, and swine. vitreous carbon buttons were also investigated as to their potential. over 200 individual implants were made and some retained in place for 2 yrs. only the nylon and dacron ... | 1975 | 124976 |
| biological properties of antibodies against rat adipocyte intrinsic membrane proteins. dependence on multivalency for insulin-like activity. | antisera from rabbits injected with rat adipocyte plasma membranes or intrinsic proteins from such membranes, obtained by a dimethylmaleic anhydride extraction step, mimicked the action of insulin on both glucose transport and lipolysis in intact adipocytes. biological activity in both types of antisera was mediated by immunoglobulin binding to one or more intrinsic proteins of the adipocyte plasma membrane since fat cells were unresponsive to all antisera absorbed with dimethylmaleic anhydride- ... | 1979 | 107169 |
| fast black b as a substitute for bis-diazobenzidine to couple proteins to erythrocytes. | | 1979 | 107241 |
| production and evaluation of antisera for serological type determination of group-b streptococci by double diffusion in agarose gel. | a double diffusion technique for serological typing of group-b streptococci is presented. the method is valuable both for type determination on a large scale, as well as for evaluation of the specificity of type antisera. as in previously published methods, hc1-extracted antigens are used. the preparation of type antisera is described and the reactions of some selected sera are given in figures. the advantages of the method are good economy of antisera and easy recognition of weak and/or unspeci ... | 1979 | 108920 |
| lyme arthritis: correlation of serum and cryoglobulin igm with activity, and serum igg with remission. | forty-eight patients with erythema chronicum migrans (ecm) were studied prospectively for 6 to 18 months. twenty-six patients had no later symptoms, but 22 subsequently developed lyme arthritis and 9 of them also experienced neurologic abnormalities. eighty-seven percent of patients with active ecm followed by subsequent involvement had cryoglobulins containing igm compared to only 13% of those with active ecm and no later symptoms. the former group also had significantly lower igg, c3 and c4 le ... | 1979 | 109097 |
| antibody to an artificial disaccharide antigen cross-reactive with neisseria gonorrhoeae lipopolysaccharide. | an artificial antigen was prepared from 4-o-beta-i-galactopyranosyl-d-glucose (lactose) and 8-ethoxycarbonyloctanol. covalent attachment to bovine serum albumin provided an antigen that elicited antilactose antibody in rabbits and goat. these antibodies were active against neisseria gonorrhoeae lipopolysaccharide in passive hemagglutination tests. the same antibody agglutinated cells of streptococcus faecalis, strain n, and precipitated the lactose-containing cell wall diheteroglycan of this org ... | 1979 | 110427 |
| in vivo effects of antiserum to igd on surface immunoglobulins, serum immunoglobulins and lymphocyte blastogenesis in rhesus monkeys. | the effects of injecting monkeys with goat antiserum to igd, the igg fraction of that antiserum or normal goat serum (ngs) were compared. the subcutaneous injection of 4 ml/kg of the whole antiserum resulted in decreased percentages of lymphocytes with surface igd or igm lasting from day 1 through day 7 post-injection followed by substantial recovery on day 10. lymphocytes from these animals were stimulated as indicated by the increased incorporation of 3h-tdr by cells placed in culture on days ... | 1979 | 112042 |
| in vitro metabolism of red blood cells from newborn and adult goats. | the erythrocytes of newborn and adult goats were studied with respect to gsh stability, gsh regeneration, levels of atp and 2, 3-dpg, glucose consumption and activities of nine different enzymes of embden-meyerhof and pentose phosphate pathways of glucose metabolism. red cells from newborn goats had significantly higher levels of atp and 2, 3-dpg. the activities of all the enzymes measured were also significantly greater in the newborn. it is suggested that newborn goats possess red blood cells ... | 1976 | 129176 |
| characterization of a t-lymphocyte inhibitor in the serum of tumour-bearing mice. | sera from mice with large tumours from a variety of tissue types and sources have been shown to contain substances capable of suppressing the proliferative response of normal mouse lymphocytes to concanavalin a (con a), bacterial endotoxin (lps) and allogeneic cells. the present paper deals with studies on the nature of these inhibitory materials using mainly a methylcholanthrene-induced rhabdomyosarcoma in dba/2j mice. it was found that a material responsible for inhibition of the con a respons ... | 1976 | 131782 |
| hypothermic effect of cocaine in rats. | cocaine injected intraperitoneally into rats resulted in a dose-dependent hypothermia. intracerebral injection of smaller doses also produced a fall in body temperature. in rabbits and guinea-pigs, cocaine produced hyperthermia, in mice and chicks it produced hypothermia while inconsistent changes were produced in goats. pre-treatment of rats with 6-hydroxydopamine, alpha-methyl-m-tyrosine or haloperidol significantly antagonized the cocaine hypothermia. pre-treatment of the rats with either hyo ... | 1975 | 132013 |
| fatal immune complex glomerulonephritis without deposits. | repeated intravenous injections of egg albumin in rabbits produced small antigen-excess complexes and severe glomerulonephritis. immunoglobulins and complement in the glomeruli were not clearly demonstrated by immunofluorescence; deposits were found to be sparse by electron microscopy. this study demonstrates that soluble immune complexes are responsible for the glomerular reaction. the apparent absence of deposits is thus not sufficient to exclude an immune complex pathogenesis. | 1975 | 123971 |
| the regulatory principles of glycolysis in erythrocytes in vivo and in vitro. a minimal comprehensive model describing steady states, quasi-steady states and time-dependent processes. | a simple mathematical model for glycolysis in erythrocytes is presented which takes into account atp synthesis and consumption. the system is described by four ordinary differential equations. conditions in vivo are described by a stable steady state. the model predicts correctly the metabolite concentrations found in vivo. the parameters involved are in agreement with data on the separate steps. the metabolite changes found in pyruvate kinase-deficient erythrocytes and the species variations am ... | 1976 | 132930 |
| a novel method in enzyme immunoassay: maleimide derivative of hapten for enzyme coupling. | meta-maleimidobenzoyl derivative of l-thyroxine methyl ester (mbtm) was synthesized and coupled to beta-galactosidase at molar ratio of over 5 to 1. more than 97% of the enzyme was found to be labeled with mbtm. a thyroxine enzyme immunoassay was carried out with sensitivity in 0-10 microgram/100 ml range. | 1979 | 108135 |
| changes in the activity of lactose synthetase in the goat udder during pregnancy. | the lactose synthetase activity of homogenates and particulate fractions prepared from the udders of goats killed at various stages of pregnancy were determined. both components of the enzyme, galactosyltransferase and alpha-lactalbumin, became detectable about half way through the gestation period, at the time when rapid proliferation of mammary epithelial tissue commences. throughout the second half of pregnancy the udder possessed a high level of lactose synthetase activity though the lactose ... | 1979 | 108321 |
| a comparison of different enzyme-antibody conjugates for enzyme-linked immunosorbent assay. | calf intestinal alkaline phosphatase (ciap)- and horseradish peroxidase (hrp)-linked goat antibody (ab) conjugates have been prepared by two procedures. one procedure is by glutaraldehyde coupling of the proteins; the other is by intermolecular disulfide bond formation of the appropriately modified proteins. the conjugates, specific for rabbit igg, were tested for their effectiveness as reagents in enzyme-linked immunosorbent assays for ragweed antigen e specific rabbit antibody. the ciap-ab con ... | 1979 | 113461 |
| prostaglandin f2 alpha-induced prolactin release and luteolysis in the goat. | injection of prostaglandin f2 alpha (pgf2 alpha) initiated a significant increase in plasma prolactin levels in all goats except those in anoestrus. luteolysis occurred in non-pregnant goats during the mid luteal phase when the goats were given pgf2 alpha either with or without the suppression of prolactin release by bromocryptine (cb154). luteolysis and subsequent parturition also occurred in pregnant goats in mid and late gestation after pgf2 alpha injection, with an associated release of prol ... | 1979 | 114152 |
| outer membrane antigens of neisseria meningitidis group b serotype 2 studied by crossed immunoelectrophoresis. | this study shows that the capsular polysaccharide, protein, and lipopolysaccharide antigens from the outer membrane of neisseria meningitidis group b serotype 2 may be identified by crossed immunoelectrophoresis. by using this technique, seven precipitates were resolved when outer membrane preparations were reacted against goat anti-whole cell group b type 2 antiserum. most of these precipitates were identified by comparison with purified reference preparations. different outer membrane preparat ... | 1979 | 115791 |
| spectrin rearrangement early in erythrocyte ghost endocytosis. | the endocytic vacuoles induced in white ghosts were found to be depleted of spectrin and therefore it was proposed that they arose from spectrin-free areas in the erythrocyte membrane. to follow changes in spectrin distribution during endocytosis, affinity-purified rabbit antispectrin antibodies were produced. quantitative techniques were developed for the use of a highly specific 125i-f(ab')2 antispectrin, and these showed that before the appearance of vacuoles, as assessed by phase microscopy, ... | 1979 | 117012 |
| diffusion in gel-enzyme linked immunosorbent assay (dig-elisa): a simple method for quantitation of class-specific antibodies. | a new method for quantifying class-specific antibodies is presented. the method has been named diffusion-in-gel-enzyme-linked-immunosorbentassay (dig-elisa), and is briefly as follows. antiserum ia allowed to diffuse from wells in a gel layered over an antigen-coated plastic surface. the gel is then removed and the preparation is incubated with enzyme-conjugated anti-immunoglobulin. the enzyme is then visualised in situ by a colour reaction produced by pouring a substrate-containing gel over the ... | 1979 | 117055 |
| a new estimate of sequence divergence of mitochondrial dna using restriction endonuclease mappings. | a new estimate of the sequence divergence of mitochondrial dna in related species using restriction enzyme maps is constructed. the estimate is derived assuming a simple posisson-like model for the evolutionary process and is chosen to maximize an expression which is a reasonable approximation to the true likelihood of the restriction map data. using this estimate, four sets of mitochondrial dna data are analyzed and discussed. | 1979 | 117114 |
| in-vitro synthesis and release of immunoglobulins by thymocytes of inbred rats. | | 1979 | 118107 |
| isolation and purification of bovine igm by dissociating immunoglobulin-brucella complexes. | methods for the dissociation of immunoglobulin-brucella complexes (igm-rich) were evaluated. the antigen-antibody complex was separated from serum by centrifugation and dissociated with either h2o, 2.0 m ph 2.0 glycine buffer, or various concentrations of kscn. optimal antibody recoveries for kscn were obtained using 1.0--2.0 m kscn. dissociation of the complex with water resulted in the highest recovery of antibody and the glycine buffer was lowest. immunoelectrophoretic analysis of the eluted ... | 1979 | 118220 |
| immunoperoxidase demonstration of human serum globulin binding to islet tissue. | sera from juvenile diabetic and nondiabetic controls were tested, using the immunoperoxidase method, for the presence of islet-binding immunoglobulins. all diabetic sera tested contained an islet-binding protein and on the average the sera were more positive than age-matched controls. normal adult sera are undifferentiated from juvenile diabetic sera. most sera from children less than two years of age did not bind to islet tissue and sera from cystic fibrosis patients had a markedly diminished a ... | 1975 | 123515 |
| studies on sarcocystis species. iv: a species infecting dogs and goats; development in goats. | | 1979 | 119935 |
| effect of increased levels of urea in the diet on ruminal protozoal counts in four ruminant species. | | 1979 | 120355 |
| effects of allo-immunization of goat bucks with goat-testes homogenates on spermatogenesis sperm motility and production of cross reacting antibodies. | | 1979 | 120664 |
| xenograft rejection in human beings. | | 1975 | 122872 |
| clinical, histologic, and histochemical study of imidocarb diproprionate toxicosis in goats. | the toxic effects of imidocarb diproprionate (3,3'-bis [2 imidazolin-2yl]-carbanilde diproprionate) were evaluated in adult goats given (intramuscular injection) a lethal dose (6.75 mg/kg). the immediate clinical signs of toxicosis were transient excessive salivation and diarrhea. anorexia, dyspnea, recumbency, and death occurred between postinjection days (pid) 4 and 8, during which time 7 goats died and 4 moribund goats were euthanatized. there were marked increases in mean serum urea nitrogen ... | 1976 | 132883 |