| autoantibodies to prolactin-secreting cells of human pituitary. | a new autoantibody which reacted with anterior-pituitary tissue was deteected by immunofluorescent staining. of 287 patients having one or more autoimmune endocrine diseases, sera from 19 reacted with pituitary glands obtained at hypophysectomy for breast cancer. using specific rabbit antibodies to each of the six pituitary hormones and rhodamine-labelled goat-anti-rabbit-immunoglobulin (ig) followed on the same unfixed cryostat section by a patients's serum counterstained with fluorescein-conju ... | 1975 | 49739 |
| evidence for specific suppression in the maintenance of immunologic tolerance. | specific suppressor cells have been demonstrated in mice tolerant to the thymus-dependent antigen hgg. transfer of normal thymocytes, normal spleen cells, or immune spleen cells into these tolerant mice did not restore immunocompetence to hgg. furthermore, the transfer of tolerant spleen cells into normal recipients abrogated the response of these recipients to subsequent challenge with immunogenic hgg. spleen cells removed from mice 5, 8, or 11 wk after the induction of tolerance specifically s ... | 1975 | 46917 |
| soil ameba infection. specific indirect immunoenzymatic (peroxidase) staining of formalin-fixed paraffin sections. | this report describes the use of the immunoenzymatic (peroxidase) method to identify the species and to stain distinctively the amebas in formalin-fixed paraffin-mounted sections. this permits the use of hematoxylin and eosin counterstaining. the method, now well developed by others for many purposes, is an alternative to immunofluorescence and seems to offer a number of advantages and a lesser number of disadvantages. | 1975 | 47225 |
| allotypic suppression in rabbits: competition for target cell receptors between isologous and heterologous antibody and between native antibody and antibody fragments. | neonatal injection with various foreign proteins (normal goat serum, human cohn fraction ii, human albumin) caused a stimulation of immunoglobulin synthesis. this effect was not antigen-specific and did not constitute a conventional antibody response directed against the injected substance. when this stimulatory effect of foreign protein was minimized, the heterologous (goat) anti-rabbit allotype antibody and rabbit antibody f(ab') 2 fragments not only failed to induce suppression but also compe ... | 1975 | 47358 |
| compound d+y+ particles of australia antigen. | australia antigen (hb8 ag) particles vary in their antigenic structure. they are generally found to carry either determinant d or determinant y, but not both. this report describes seven sera which contain australia antigen carrying both d and y on the same particle. | 1975 | 47365 |
| a hemolytic plaque assay for the detection of direct and indirect antibody-forming cells to keyhole limpet hemocyanin. | a procedure is described for the in vitro enumeration of individual lymphoid cells producing antibody against keyhole limpet hemocyanin (klh) by a modification of the hemolytic plaque technique. optimal conditions for the coupling of klh to sheep erythrocytes by chromic chloride are described. plaque-forming cells are detected in a liquid monolayer slide chamber assay system. the kinetics of the in vivo primary and secondary pfc responses of both rabbit popliteal lymph node cells and mouse splee ... | 1975 | 47369 |
| immunological and electrophoretical approaches to macroamylase analysis. | the characteristics of abnormally large-size amylase (macroamylase) of the three patients were studied. amylase isoenzyme patterns of the three macroamylase samples of agar gel electrophoresis were different from the normal one: a post-beta-globulin or middle-ganna-globulin zone was found in macroamylase, while a fast-gamma, and a pre-gamma-globulin or both zones is found in normal amylase. with gel chromatography at ph 3.4, macroamylase dissociated to normalsized amylase. the electrophoretic pa ... | 1975 | 47791 |
| antihistamine blockade of the ovarian hyperstimulation syndrome. ii. possible role of antigen-antibody complexes in the pathogenesis of the syndrome. | antihistamine can prevent the ovarian hyperstimulation syndrome in the rabbit. the mechanism underlying this experimental observation has not been elucidated. our experiments were directed at the question of whether antigen-antibody complexes are a factor in the development of the ovarian hyperstimulation syndrome. the results do not support the theory that antigen-antibody complexes play a role in the pathogenesis of this syndrome. | 1975 | 47816 |
| testis antigens of man and some other primates. | rabbit antisera raised aginst testis preparations of human, chimpanzee, rhesus monkey, and baboon origin were used to study testis-specific antigens within and among the four primate species. antisera were absorbed with serum, liver, kidney, and spleen preparations of the respective species against which they had been produced. immunoelectrophoretic analysis of testis extracts, using the absorbed antisera, indicated the following minimum numbers of testis-specific antigens for each species: man, ... | 1975 | 47818 |
| hepatitis b core antigen. detection of antibody by radioimmunoprecipitation. | radioactive cores were prepared from concentrated dane particles by dna polymerase reaction followed by cscl density gradient centrifugation. two radioactive peaks were obtained: one peak with an average density of 1.36 g/cm-3 in cscl contained cores that possessed full serologic reactivity; the other peak, with a density of 1.28 to 1.32 g/cm-3, contained cores associated with globulin. a double antibody immunoprecipitation test was developed, using the radioactive heavy cores as a source of ant ... | 1975 | 47880 |
| induction of resistance to antibody-mediated cytotoxicity. h-2, ia, and ig antigens are independent entities in the membrane of mouse lymphocytes. | mouse spleen or thymus lymphocytes incubated with monospecific h-2 or ia alloantisera and then coated with a xenogeneic antimouse ig serum become specifically resistant to the alloantiserum (and complement) they have been incubated with. this so called "lysostrip method" was used to investigate the molecular interrelationships of antigens in the mouse lymphocyte membrane. the results of this investigation confirm that h-2k and h-2d antigens are carried by two distinct populations of molecules. t ... | 1975 | 47892 |
| reaginic antibody in cattle hypersensitised by foot-and-mouth disease vaccine. | the passive cutaneous anaphylaxis test (pcat) in calves and goats was used to demonstrate reaginic antibody in the sera of cattle which had shown anaphylaxis following injection with foot-and-mouth disease vaccine. a period of two or three days between the intracutaneous injection of test sera and the intracutaneous or intravenous challenge with components of vaccine was found to be satisfactory. positive reactions were obtained in calves for up to 49 days but many goats failed to react after ei ... | 1975 | 50615 |
| immunology of the prostate. | | 1975 | 48210 |
| antibodies reactive with cell surface carbohydrates. | normal and immune sera from various animal species were fractionated on columns of sepharose covalently coupled with the glycoprotein fetuin. elution of the material bound to fetuin yielded low but reproducible amounts of protein, ranging from 0.02 to 0.2% of the protein mass of the input sera. this material has been identified by immunoelectrophoresis in agar and by zone electrophoresis on cellulose acetate as immunoglobulin. the ig fractions bound and agglutinated erythrocytes of various speci ... | 1975 | 48249 |
| glomerular antigen complexes associated with transitional cell carcinoma. | an immunofluorescent pattern consistent with glomerular complexes of tumor antigen bound to igm antibody has been observed in a patient without clinical or pathologic evidence of renal disease. the findings support the thesis that tumors may shed antigen capable of eliciting an immune response which may or may not be associated with clinically evident glomerulopathy. | 1975 | 48285 |
| the binding of carcinoembryonic antigen by antibody and its fragments. | in order to assess the potency of antigenic fragments of carcinoembryonic antigen (cea) in the radioimmune assay; it is necessary to know whether the high affinity of goat anti-cea antibody (which makes possible the detection of as little as 10--11m cea) is due to bivalent binding of the cea molecule. immunoglobulin g and the f(ab')2 and fab fragments derived from it were prepared from an anti-cea serum and tested for their abioity to bind cea. equivalent concentrations of binding sites of the b ... | 1975 | 48422 |
| [biochemical properties of alpha-fetoprotein (author's transl)]. | | 1975 | 51943 |
| comparative studies of thyroid function in mammals. | | 1975 | 48491 |
| lactoglobulins (goat) in the immunofluorescence staining of parainfluenza virus type 2 in l-132 cells. | instillation of parainfluenza virus type 2 in the mammary gland of the goat resulted in the appearance of specific and high titered antibodies in the milk. the globulin fraction of the milk was separated and conjugated with fluorescein isothiocyanate. these conjugated antibodies could be successfully used in the specific staining of the homologous virus in infected l-132 cells. therefore, milk from such immunized animals represents a rich and convenient source of specific antibodies for use in i ... | 1975 | 49216 |
| specific fetal serum proteins of thirteen mammalian species. | | 1975 | 49249 |
| isolation of mammalian type c rna virus cross-reactive antigen and antibody by immuno-affinity chromatography. | | 1975 | 49291 |
| human antibodies to bovine alpha-globulin. | antibodies to bovine gamma-globulin (anti-bgg antibodies) were detectable by a radio-immunoassay in 70% of healthy blood donors but, generally, the titres were low. significantly increased concentrations of anti-bgg antibodies were found in patients lacking iga but not in patients with allergic disorders. the anti-bgg antibodies were shown to give rise to falsely high ige values in the radio-immunosorbent test for ige determination (rist) when a sheep anti-ige antiserum was used. furthermore, fa ... | 1975 | 49305 |
| distribution of labeled antibiotics in different components of milk following intramammary and intramuscular administrations. | in crossover trials, four lactating goats were given intramammary infusions and intramuscular injections of radioactivelabeled benzylpenicillin, spiramycin, chloramphenicol, dihydrostreptomycin, and tetracycline. milk was collected after each treatment and the antibiotic contents in whole milk, skim milk, and whey were determined microbiologically and radiochemically and in cream and casein by radiochemical assay methods. uptake of antibiotics by cream and casein was highly dependent on drug con ... | 1975 | 49365 |
| [immunohistochemical studies on non neoplastic and neoplastic gastric mucosa. determination of embryonic and specific antigens (author's transl)]. | the distributions of acid alpha1-glycoprotein, alpha1-fetoprotein, beta-galactosidase and gastrin in gastric carcinoma and gastric ulcer as well as in the neighbourhood of these lesions were studied by means of immunohistochemical methods on imprint preparation. we could not find significant differences between gastric carcinoma and the nonneoplastic lesions, except for the acid alpha1-glycoprotein. the results of this first study indicate that the immunochemical and immunohistological assay of ... | 1975 | 52951 |
| preparation and evaluation of antisera directed against cancer specific moiety of antigenic determinants on carcinoembryonic antigen. | | 1975 | 53193 |
| multiple antigenic sites on an eicosapeptide. i. precipitin studies in the goat. | purified peptide 105-124, an antigenic determinant from the carboxy terminus ribonuclease, was found to form an immune precipitate with antibody to that region prepared by affinity chromatography from goat hyperimmune antiserum to reduced carboxymethylated ribonuclease (cm-rnase). cm-rnase also gave an immune precipitate with the antibody. purified antibody to another region of similar size (40-61) did not form a precipitate with cm-rnase but did co-precipitate in the presence of antibody to pep ... | 1975 | 53198 |
| serodiagnostic application of immunohistoperoxidase reactions on antigen-coupled agarose beads. | agarose beads to which antigens were covalently bound were subjected to indirect immunohistoperoxidase procedures. for detection and titration of serum antibodies against bovine gamma globulin (bgg) this method appeared to be specific and sensitive. one advantage is that no special instruments are needed. as an example of diagnostic applicability the system was successfully used for demonstration of antibodies in human serum containing antibodies against the trematode schistosoma mansoni. at the ... | 1975 | 49378 |
| molecular relationship between private and public h-2 antigens as determined by antigen redistribution method. | molecular relationship of public h-2 antigens 1, 5, 6, 8, 11, 13, 25, and 28 to private antigens controlled by k and d regions was studied using the technique of antibody-induced resistance to complement-mediated cytotoxicity. the results indicate physical association in the cell membrane between h-2 antigens 1 and 23 of h-2-a, 8 and 31 of h-2-d, 11 and 17 of h-2-q, 13 and 30 of h-2-q, 25 and 23 of h-2-k, and 28 and 31 of h-2-g. these results are in agreement with genetic mapping placing the d ... | 1975 | 49386 |
| [autoantibodies against heartmuscle sarcolemma in sera of patients with an idiopathic cardiomyopathy (author's transl)]. | 68 patients with clinical and hemodynamical diagnosis of idiopathic cardiomyopathy (cm) were analysed for antibody in their sera using an immunofluorescence sandwich technique after titration of the sera in tissue sections. 60 of these patients had antibodies against heart muscle sarcolemma with titers up to 1:64. out of these, the antibodies in 9 sera could be absorbed with group a streptococci. the antibodies reacted in no case with striated or smooth muscle sarcolemma. the 25 patients with an ... | 1975 | 49452 |
| binding of 14-c-salicylic acid and 14-c-pentobarbital to plasma proteins of several species during the perinatal period. | the fraction binding of 14-c-salicylic acid and 14-c-pentobarbital was studied as a function of age in plasma of the pig, dog, goat and human. the pig exhibited an unusual degree of hypoalbuminemia and low fractional binding of salicylic acid at birth. albumin levels and the percent binding of the salicylic acid were not significantly lower in newborn plasma than in adult plasma of the other species studied. the pig was also exceptional in that the high fractional binding of pentobarbital observ ... | 1975 | 50090 |
| serological relationship of taxonomically closely related domestic ruminants: cattle, buffalo, sheep & goat. | | 1975 | 54334 |
| heterogeneity of human circulating anticoagulants against antihemophilic factor (factor viii). | the heterogeneity of human circulating anticoagulants against antihemophilic factor (ahf, factor viii) observed in seven patients, both with and without classic hemophilia, was investigated by neutralization of their activity with antiserums directed to whole igg and to lambda and kappa light chains. all seven anticoagulants were immunoglobulins. six appeared to contain both kinds of light chains, although the dual light chain composition of two of these could be demonstrated only at high concen ... | 1975 | 50101 |
| the differences in atrioventricular conduction of premature beats in young and adult goats. | atrioventricular (av) conduction was studied in anesthetized, open-chest, 1-35-day-old goats. atrial, bundle of his, and ventricular bipolar electrograms were recorded, and the functional refractory periods of the av conduction system and the ventricles were determined. supraventricular premature excitation invaded the ventricles during their vulnerable period. this av conduction property is different from that of the adult goat heart; thus, the existence of a lateral accessory bypass tract was ... | 1975 | 50153 |
| electron microscopic immunohistochemical identification of endothelial cells in the rabbit. | antibody to tissue factor apoprotein was adsorbed against gamma-globulin and coupled to horseradish peroxidase; this complex was applied to various rabbit tissues. the distribution of the peroxidase marker then was observed by electron microscopy. we examined fixed or frozen sections, as well as breis of aorta, vena cava, brain, heart, lung, liver, spleen, kidney, bone marrow, mesothelial gut lining, erythrocytes, and platelets. all endothelial cells that had been exposed to the antibody complex ... | 1976 | 55315 |
| chemistry of a low molecular weight ragweed pollen allergen ra5, and association of human responsiveness to ra5 with hl-a7 cross-reacting group. | a protein allergen, ra5, was isolated by column chromatographic procedures from the aqueous extract of short ragweed pollen by lapkoff and goodfriend. the allergen was obtained in a highly homogenous form, was devoid of carbohydrate and had the low molecular weight of 5100 daltons. a report will be given of studies by marsh, bias, hsu and goodfriend on the cutaneous activity of ra5 in 105 antigen e-sensitive patients along with hl-a typing of these patients. the results have demonstrated a highl ... | 1975 | 50245 |
| immunochemical localization of in vivo bound immunoglobulins in pemphigus vulgaris epidermis. employment of a peroxidase-antiperoxidase multistep technique for light and electron microscopy. | a multi-step immunocytochemical method utilizing horseradish peroxidase (hrp) as an immunological marker was employed to demonstrate, at the ultrastructural level, igg antiepithelial autoantibodies bound in vivo to pemphigus epidermis, and circulating igg antibodies, using monkey oesophagus as substrate. to demonstrate igg the following antisera were employed in sequential steps: goat antihuman igg; rabbit antigoat igg; goat-anti-hrp-serum. after incubating with the final antigen, hrp, the latte ... | 1975 | 56157 |
| semi-automatic solid-phase double-antibody radioimmunoassay for beta2-microglobulin. | | 1976 | 56306 |
| immunological heterogeneity of serum carcinoembryonic antigen (cea). | | 1976 | 56309 |
| formation of an infectious virus-antibody complex with rous sarcoma virus and antibodies directed against the major virus glycoprotein. | preparations of rous sarcoma virus (rsv) can form an infectious viral-antibody complex with antibodies raised against the major glycoprotein, gp85, isolated from avian myeloblastosis virus and prague-rsv subgroup c. binding of anti-gp85 antibodies to rsv can be demonstrated by the inhibition of focus-forming activity after addition of goat anti-rabbit immunoglobulin and by a shift in density of virions treated with anti-gp85 serum. group- rather than subgroup- specific regions of viral gp85 appe ... | 1976 | 56458 |
| alpha-fetoprotein detected in rat transplantable hepatomas by radioimmunoassay. | production of rat alpha-fetoprotein by transplantable ascites hepatoma was studied by radioimmunoassay method. alpha-fetoprotein was detected in sera from rats bearing 22 out of 50 ascites hepatoma cell lines that were previously negative for the presence of alpha-fetoprotein by the ouchterlony test. alpha-fetoprotein was also detected with high prevalency in morris hepatomas and sublines of yoshida sarcoma by this assay system. | 1975 | 50249 |
| radioimmunoassay of human serum antibody specific for adenovirus type 5-purified fiber. | a radioimmunoassay (ria), utilizing a second antibody to separate immune complexes, was developed to provide a sensitive and specific measure of serum antibody to adenovirus type 5 (ad 5) fiber. purity of fiber antigen was ascertained by sodium dodecyl sulfate urea-polyacrylamide gel electrophoresis and isoelectric focusing in ampholyte ph gradients. after labeling with 125i to high specific activity, the iodinated fiber did not exhibit loss of antigenic reactivity and remained stable for 3 week ... | 1975 | 50343 |
| receptors for antigen on lymphoid cells. ii. the nature of the molecule responsible for plaque-forming cell adherence. | the nature of specific adherence of rat anti-tnp pfc to tnp-grbc has been investigated with pll-fixed antigen monolayers as cellular immunoadsorbents and as plaque indicators. the immunoglobulin nature of the molecule responsible for pfc adherence is suggested by the fact that pretreatment of the pfc with rabbit anti-rat ig antisera, but not anti-histocompatibility antisera, inhibits adherence. removal of the adherence capacity of early pfc with the proteolytic enzymes papain and pronase, or by ... | 1975 | 50360 |
| a surface membrane determinant shared by subpopulations of thymocytes and b lymphocytes. | utilizing a quantitative fluorescence assay with the fluorescence-activated cell sorter (facs), we have demonstrated that a rabbit antiserum obtained by immunization with cells of a mouse igm-producing plasma cell tumor (mopc104e) is reactive with at least two surface determinants, designated th-b and ml2, on subpopulations of normal murine lymphocytes. the ml2 determinant is restricted to b lymphocytes. the th-b determinant is shared by splenic b lymphocytes and a large subpopulation of thymocy ... | 1975 | 50365 |
| further studies on the biologic properties of guinea pig igg1 antibodies. ii. in vivo activation of c3 by anti-glomerular basement membrane antibodies. | normal rats were injected with guinea pig anti-rat glomerular basement membrane antibodies of the igg1 or igg2 class or with their f (ab') 2 fragments, in order to study which antibody site triggers the alternate complement pathway in vivo. both igg classes were able to induce a heavy proteinuria and led to c3 deposition in the glomeruli in a pattern similar to their own distribution along the glomerular basement membrane, as shown by the immunofluorescence technique. the fab(ab')2 fragment of i ... | 1975 | 50374 |
| antigenic specificity of red and white muscle myosin. | | 1975 | 47867 |
| ultrastructural and immunohistochemical studies in five cases of argentine hemorrhagic fever. | ultrastructural and immunohistochemical studies on tissues from five patients with argentine hemorrhagic fever revealed previously undetected lesions caused by the viral infection. two types of particle were seen in the cells of all organs examined. the particles had some characteristics similar to those described for arenaviruses. however, the virus-like particles were intracellular, had a single membrane, and apparently originated by a process of budding into the endoplasmic reticulum cisterna ... | 1975 | 50390 |
| association of beta-2-microglobulin and hl-a in the cell membrane. a general property in human cells? | to study whether the recently reported close association of beta-2-microglobulin and hl-a determinants is restricted to lymphocyte membranes or might be a more general property, experiments were performed where normal and malignant human cells were tested for coaggregation-capping of the two determinants. the normal cells were fibroblasts and the epithelial cells were from skin. the turmor cell lines were hela clone s3 carcinoma cells and national collection of type cultures strain 3075, which i ... | 1975 | 50654 |
| isolation and preliminary characterization of two varieties of low molecular weight immunoglobulin in the bullfrog, rana catesbeiana. | two varieties of low m.w. immunoglobulins have been isolated from the serum of rana catesbeiana frogs. they are highly cross-reactive, although each also contains unique antigenic determinants. since both low m.w. immunoglobulins were identified in the serum of 22 individual frogs, it was concluded that they are isotypic variants. the light chains of r. catesbeiana and mammalian high and low m.w. immunoglobulins are similar in electrophoretic mobility on polyacrylamide gels containing sodium dod ... | 1976 | 59776 |
| cross reactivity of human beta2-microglobulin with human granulocyte colony-stimulating activity. | effects of antisera to human beta2-microglobulin (beta2 m) on factors able to stimulate colony formation in culture by human granulopoietic progenitor cells were investigated. the colony-stimulating activity (csa) present in media conditioned by cultures of human peripheral leukocytes was suppressed by treatment with anti-beta2m. this inhibition was not due to a direct effect on the granulopietic progenitor cells; controls to test for cytotoxicity and for noncytotoxic inhibition of the progenito ... | 1976 | 59777 |
| purification of canine plasma renin by affinity chromatography. | an affinity column for the purfication of canine plasma renin was prepared using goat anti-renin (dog kidney) gammag gloublins. the antiserum was prepared against a purified kidney renin preparation. the anti-renin globulins were coupled to cyanogen bromide activated sepharose. using the anti-renin globulin-coupled sepharose as an immuno-adsorbant, a method was devised allowing purification of plasma renin to a 1,000-fold purity. | 1976 | 59924 |
| isolation of hl-a 33,000-dalton fragments carrying high hl-a alloantigenic activity. | | 1975 | 50658 |
| studies on the immunosuppressive properties of a pregnancy-associated alpha-macroglobulin. | a pregnancy-associated serum glycoprotein was shown to have an inhibitory effect on several in vitro methods of immunological assessment. this suppressing activity was evident at physiological concentrations and did not appear to be due to cytotoxicity. transformation, induced by agents often regarded as preferential stimulators of t lymphocytes (concanavalin a, phytohaemagglutinin, allogeneic cells and tuberculin) was significantly depressed by the alpha-globulin. however, this phenomenon was m ... | 1976 | 60185 |
| studies on mouse moloney virus induced tumours: i. the detection of p30 as a cytotoxic target on murine moloney leukaemic spleen cells, and on an in vitro moloney sarcoma line by antibody mediated cytotoxicity. | antigenic determinants of p30, the most abundant internal virion protein of c type rna viruses, were detected on the surface of spleen cells from mice bearing moloney leukaemia and on an in vitro line of moloney sarcoma, msc. on both cell types, these determinants on the p30 molecules served as cytotoxic targets in a xenogenic complement dependent antibody mediated 51cr release assay. two antisera were used: a rat anti mlv -m induced lymphoma serum, and an antiserum raised in goats to either dis ... | 1975 | 50852 |
| studies on mouse moloney virus induced tumours: ii. detection of p30 in the serum of mice with moloney leukaemia by in vitro blocking of complement dependent antibody mediated cytotoxicity. | sera from balb/c mice bearing moloney leukaemia block complement dependent antibody mediated cytotoxicity of an antiserum prepared in rats against syngeneic moloney virus induced lymphomata when either spleen cells from mice bearing moloney leukaemia (m) or an in vitro line of moloney virus transformed cells (msc) are used as targets. this antiserum has been shown to recognize p30, the major internal virion protein, as a cytotoxic target on these cells. viral particles were identified by electro ... | 1975 | 50853 |
| immunological activity against tick-borne encephalitis virus in fractions of sera from goats of different ages. | a comparative analysis of the antihaemagglutinating, virus-neutralizing and complement-fixing activities of 19 s, 7 s, 4s and 1.5 s fractions of blood sera from goats of different ages pastured in a tick-borne encephalitis focus was carried out. changes in the immunological activity of different fractions in relation to the age of the animals are discussed. | 1975 | 52294 |
| some staining characteristics of mott's or morular cells of nagana. | the spherules of morular (mott's) cells are not metachromatic with toluidin blue at ph 8 and the majority are stained yellow with ptah. this distinguishes them from globule leucocytes. they differ from russell bodies of plasma cells which are gram-positive. | 1975 | 52355 |
| demonstration of two molecular variants of carcinoembryonic antigen by concanavalin a sepharose affinity chromatography. | the carcinoembryonic antigen (cea) active glycoproteins from perchloric acid extract of liver-metastasized primary colon tumor have been separated by concanavalin a sepharose (con a sepharose) chromatography. the cea activities separated by con a sepharose chromatography were designated as loosely bound and tightly bound which, respectively, eluted on the con a sepharose column between 0.12 and 0.15 m and 0.3 m alpha-methylmannose in a linear gradient of alpha-methylmannose. further purification ... | 1975 | 52402 |
| relationships between membrane antigens of human leukemic cells and oncogenic rna virus structural components. | leukemic cells from all human chronic granulocytic leukemia (cgl) and some acute myelomonocytic leukemia (amml) donors are lysed by rabbit antisera to a purified glycoprotein of friend murine leukemia virus (flv gp71) in a microcytotoxicity assay. these antisera are not cytotoxic to cells from patients with acute myelocytic leukemia (aml), acute lymphocytic leukemia (all), chronic lymphocytic leukemia (cll), or to peripheral blood lymphocytes from normal donors. a goat antiserum to gradient puri ... | 1976 | 53269 |
| studies of the hypothalamus and the pituitary gland with special reference to the effects of transection of the pituitary stalk. | | 1975 | 53978 |
| electron microscopic demonstration of immunoglobulin deposition in rheumatoid cartilage. | horseradish peroxidase (hrpo) conjugated with goat antihuman igg, goat antihuman igm, and aggregated human igg has been used as a enzymatic marker to stain igg, igm, and rheumatoid factor in rheumatoid cartilage. when hrpo-anti igg and hrpo-anti igm were used, immunoglobulin deposits were not observed in nonrheumatoid cartilage. however 7 of 8 rheumatoid cartilage specimens stained with hrpo-anti igg showed electron-dense deposits. three rheumatoid specimens stained with hrpo-anti igm showed sim ... | 1975 | 54168 |
| natural antibodies against colicines in human and animal sera. | the presence of "natural antibodies" against colicines was detected in the sera of 13 (29.5%) hospitalized recruits aged 20-29 years, 4 (25%) hospitalized males aged 30-49 years, 8 (40%) outpatient elderly subjects aged 50-79 years and in the sera of 15 (27.2%) animals. it is discussed the possible role of colicines as antigenic determinants stimulating the production of "natural antibodies", because of the prolonged and repeated contact of colicinogenic strains with the host animals. | 1975 | 54185 |
| species and interspecies radioimmunoassays for rat type c virus p30: interviral comparisons and assay of human tumor extracts. | the major internal protein, p30, of rat type c virus (ralv) was purified and utilized to establish intra- and interspecies radioimmunoassays. three rat viruses were compared in homologous and heterologous intraspecies assays with no evidence of type specificity. the only heterologous viruses to give inhibition in these species assays were the feline (felv) and hamster (halv) type c viruses; these reactions were incomplete and required high virus concentrations. an interspecies assay using a goat ... | 1975 | 54444 |
| letter: escherichia coli k1. | | 1976 | 54718 |
| role of histamine in the genesis of pyrogen (endotoxin)-induced reticulo-ruminal stasis in goats. | | 1976 | 64092 |
| evidence for more than one ia antigenic specificity on molecules determined by the i-a subregion of the mouse major histocompatibility complex. | ia antigenic specificities determined by the i-a subregion of the mouse major histocompatibility complex have been examined in strain b10.d2 (h-2d), c57bl/10 (h-2b), and in a (c57bl/6xdba/2) hybrid (bdf1; h-2b/d). detergent solubilized, 3h-leucine-labeled antigen preparations were mixed with appropriate alloantisera and precipitation was induced either by addition of goat anti-mouse gamma-globulin or by addition of protein a-bearing staphylococci. sequential precipitation analysis showed that in ... | 1976 | 55444 |
| editorial: failure of cerebral autoregulation in liver disease. | | 1976 | 56541 |
| existence of homologous antigenic structures in unfolded creatine kinase and arginine kinase. | | 1976 | 57067 |
| an immunoperoxidase technic for the demonstration of the hepatitis b surface antigen in human livers. | a sensitive method for demonstrating the site of hepatitis b surface antigen (hbsag) in fixed tissues embedded in either paraffin or araldite is described. the method employs the peroxidase-rabbit antiperoxidase linkage through goat antirabbit to rabbit anti-hbsag. in staining hepatitis antigen in agar, comparison of fixation (using three common fixatives) with unfixed precipitation arcs revealed no recognizable differences in antigenicity induced by fixation. the method allows confirmation of p ... | 1976 | 57716 |
| antigenic relationships among influenza virua a neuraminidase (n2) antigens by immunodiffusion and postinfection neutralization tests. | the antigenic relationships among the neuraminidases of influenza a strains from 1957 to 1973 were examined by postinfection application of neuraminidase antisera. this procedure causes inhibition of virus spread and apparent neutralization. neuraminidase (apparent) neutralization and neuraminidase inhibition tests with chicken antisera gave similar results. neuraminidase inhibition tests were more discriminating than neuraminidase neutralization tests when rabbit and goat antisera were used. an ... | 1976 | 57967 |
| neutralization of homologous and heterologous oncornaviruses by antisera against the p15(e) and gp71 polypeptides of friend murine leukemia virus. | | 1976 | 58469 |
| interspecies determinants of friend leukemia virus antigens involved in cytolysis of virus-pfoducing cells. | the cytolytic reactivity of a complex goat anti-feline leukemia virus (felv) antiserum for mouse cells (eveline) releasing large quantities of friend leukemia virus (flv) was analyzed by the sensitive [14c]nicotinamide release microcytotoxicity assay. whereas this interspecies killing reactivity could be blocked by absorption of the goat anti-felv serum with a preparation of disrupted flv, absorption with purified flv gp71, the major envelope glycoprotein, had no effect. subsequent serum absorpt ... | 1977 | 64623 |
| spectrophotometric determination of fluorophor, protein, and fluorophor/protein ratios in fluorescamine and mdpf fluorescent antibody conjugates. | | 1976 | 58564 |
| immunological relationships among hexosaminidases of different species. | | 1976 | 59695 |
| early detection of pregnancy-associated serum protein using antiserum to placental antigens. | antisera against human placental proteins were developed in goats and rabbits, using immunoadjuvants and a prolonged injection schedule. the antisera were absorbed with normal serum proteins and then tested in immunodiffusion against normal and pregnancy sera. two bands of precipitation due to pregnancy antigens were observed in pregnancy sera as early as 18 days after conception. detection of these antigens has possibilities for application as an early pregnancy test. | 1976 | 59699 |
| manganese metabolism in cows and goats. | when 54mncl2 was incubated with fresh bovine or caprine serum for 20 h and the serum subjected to electrophoresis at ph 9.5, the 54mn bound to transferrin and alpha2-macroglobulin in proportions which varied with the temperature of incubation and the temperature of electrophoresis. between 0 and 37 degrees c, the higher the temperature of incubation the larger the proportion bound to transferrin and the lower the proportion bound to alpha2-macroglobulin. the temperature at which electrophoresis ... | 1976 | 60137 |
| expression of antigenic crossreactivity to rd114 p 30 protein in a human fibrosarcoma cell line. | an antigen crossreacting with the 30,000-molecular-weight protein (p30) of the feline endogenous oncornavirus (rd114) was detected in a well-characterized human fibrosarcoma cell line, ht1080, by indirect immunofluorescence. three antisera against rd114 p30 gave similar positive results, while two antisera prepared against simian sarcoma virus p30, one antiserum prepared against murine leukemia virus p30, and one antiserum prepared against feline leukemia virus p30 gave no immunofluorescence. th ... | 1977 | 66679 |
| detection by immunofluorescence of antibodies to parasitic agents. use of class-specific conjugates. | | 1975 | 52314 |
| studies on defined immunofluorescence in clinical immunopathology. ii. relationship of f/p ratios pf conjugates and staining properties in indirect immunofluorescence. | the effect of conjugate f/p ratios on titers of patient antibodies was evaluated with two model systems in iif chessboard titrations, notably, antinuclear antibodies and the intercellular antibodies of pemphigus. the plateau titers of both antibodies and f/p conjugate ratios were analyzed and proved to have a high degree of linear relationship statistically. single titrations of other tissue antibodies, including skin basement membrane antibodies of bullous pemphigoid, mta, gpa, sma, and tha wit ... | 1975 | 52325 |
| treatment of melanoma by passive humoral immunotherapy using antibody drug synergism. | | 1977 | 68204 |
| microfluorometric evaluation of conjugate-specificity with the defined antigen substrate spheres (dass) system. | six fluorescent antihuman ig preparations were tested for their ig class specificity by reacting them with highly purified igg, igm, iga, and ova coupled covalently to sepharose beads. ova was used as a measure for nonimmunologic binding. bead fluorescence was determined by microfluorometry. the amounts of uss and nss were expressed quantitatively. these data were compared with the performance of these particular conjugates in a biologic system, namely, monoclonal bone marrow cells. five of the ... | 1975 | 52328 |
| the a(w) subdeterminants. | | 1976 | 61018 |
| a radioimmunoassay for bovine parathyroid hormone. | a radioimmunoassay for bovine parathyroid hormone (bpth) has been developed. an antibody was raised in a goat against 1-84 b pth which was directed against the carboxy-terminal part of the molecule (no cross-reactivity with synthetic 1-34 b pth fragment). 1-84 b pth was labelled with 125i using the chloramine-t method. the tubes were incubated at 4 degrees c for 6 days in an equilibrium system with 25% protein concentration. separation was performed using plasma-coated charcoal. jugular venous p ... | 1976 | 61269 |
| in vitro transmission and propagation of the bovine leukemia virus in monolayer cell cultures. | this study demonstrates that the bovine leukemia virus (blv) can infect in vitro cells of human, simian, bovine, canine, caprine, ovine, and bat origin. cultures of these cells, cocultivated with blv-infected cells or inoculated with cell-free blv preparations, continuoously showed the presence of cells with the internal blv antigen as well as blv-induced syncytia. virus replication was abundant and increased with passage in bat lung cells and was moderate but constant in fetal canine thymus cel ... | 1976 | 61801 |
| a study of possible biohazards in the fluorescent antibody test using adenovirus, coxsackievirus, herpesvirus, and respiratory syncytial virus as antigens. | infectious adenovirus type 5 and coxsackievirus type b5, both nonlipid-containing viruses, were isolated from cells fixed in acetone at 22 degrees c for 15 min, from acetone used for fixation, from the solution used for washing slides during the fluorescent antibody procedure, and after complete processing of antigen preparations with serial twofold dilutions of human antisera and fluorescein-labeled goat anti-human immunoglobulin g. lipid-containing herpes simplex virus type 1 and respiratory s ... | 1976 | 61971 |
| relationship between leukemia antigens and stimulation in mixed leukocyte culture. | leukemia cells from 13 of 27 patients stimulated lymphocytes of their hla-identical siblings in mixed leukocyte cultures (mlc). stimulation correlated with high background uptake of tritiated thymidine and high percentages of cells that stained with fluorescein-conjugated polyvalent goat antiserum against human immunoglobulin. results were similar to those reported with fractionated autologous lymphocyte subpopulations in normal individuals. investigation of leukemia-specific stimulation in mlc ... | 1977 | 69037 |
| cytomegalovirus specific igm and igg response in humans studied by radioimmunoassay. | an indirect solid phase micro-radioimmunoassay (ria) was adapted for the measurement of anti-cytomegalovirus class-specific immunoglobulin m (igm) and immunoglobulin g (igg). cytomegalovirus (cmv) antigen (ag) was added to the wells of microtiter plates and desiccated onto the bottom surface of the wells. serial dilution of human cmv antisera were added and allowed to react with the ag. the amount of viral antibody (ab) was determined by measuring the specific binding of 125i-labeled goat anti-h ... | 1976 | 62802 |
| effects of chemical modification of the protein on the antigenic activity of carcinoembryonic antigen. | | 1976 | 63400 |
| antibody-drug synergism: an assessment of specific passive immunotherapy in bronchial carcinoma. | patients with bronchial carcinoma who had had radical resection of the primary tumour were treated with a combination of drugs and tumour-specific immunoglobulin (antibody-drug synergism, a.d.s.). the antisera were raised in goats by immunisation with tumour cells from patients, and given to those patients after absorption to remove antibody against normal human tissues. 32 patients received a.d.s. treatment and 37 patients received chemotherapy only. although the differences are not statistical ... | 1977 | 69782 |
| preparation of anti-cea and anti-goat gamma-globulin sera for radioimmunologic assay of carcinoembryonic antigen. | goats were immunized with purified carcinoembryonic antigen, and the suitability of the antisera for clinical assays of carcinoembryonic antigen was characterized. reactivity of equine sera to goat gamma-globulin as a precipitating factor in the radioimmunologic double antibody technique was also evaluated. | 1977 | 70197 |
| antibody-dependent cellular cytotoxicity against murine leukemia viral antigens: studies with human lymphoblastoid cell lines and human peripheral lymphocytes as effector cells comparing rabbit, goat, and mouse antisera. | a thymic lymphoblastoid cell line derived from a new zealand black mouse produces murine leukemia virus (mulv) and was used as a target in model systems for the in vitro study of antibody-dependent cellular cytotoxicity (adcc). several human lymphoblastoid cell lines were investigated as potential effector cells. the most promising (raji cells) bound to antibody-coated target cells but caused only modest levels of adcc at 25:1 effector-to-target cell ratio with substantial lysis in the absence o ... | 1977 | 70469 |
| conformational integrity of myoglobin after immunization with freund's adjuvant. | the use of freund's complete adjuvant for immunizing goats with myoglobin produces mainly antibodies directed against antigenic determinants present in the native protein. only about 9% of the total antibodies produced are directed toward determinants not expressed in tha native molecule. this shows that neither emulsification nor the subsequent in vivo events leading up to the immune response appreciably perturb the conformation of the protein surface. | 1977 | 63516 |
| [therapeutic effect of heterologous antitoxic antistaphylococcal gamma-globulin in rabbits with experimental staphylococcal infection]. | | 1977 | 70916 |
| metabolism of dl-(delta-15n, 1-14c) ornithine in the perfused goat udder. | | 1976 | 71072 |
| antibodies against protein antigenic sites that are identical in the homologous protein of the immunized animal. autoreactivity in rabbits of antibodies to sperm-whale myoglobin. | sequence comparisons between the antigenic sites of sperm-whale myoglobin and the corresponding regions in rabbit myoglobin indicate that rabbits make antibodies to regions of the sperm-whale myoglobin molecule which are identical to the corresponding regions in rabbit myoglobin. rabbit myoglobin did not precipitate with antisera to sperm-whale myoglobin. however, it exhibited an extensive cross-reaction as demonstrated by its ability to inhibit the precipitin reaction of sperm-whale myoglobin, ... | 1977 | 71166 |
| identification of two genetic variants of goat alpha-lactalbumin. | | 1976 | 64206 |
| a simple spot technique for thin layer immunoassays (tia) on plastic surfaces. | a simple and sensitive technique for visualization of antigen--antibody reactions is described. the property of many antigens to become adsorbed firmly on to a hydrophobic polystyrene surface while retaining their serological reactivity is taken advantage of. on a surface with adsorbed antigen the corresponding immune serum is applied spot-wise. the antigen--antibody reaction areas on the surface are characterized by a distinct hydrophilic condensation pattern when exposed to water vapour. the r ... | 1977 | 71329 |
| [inventory of oligoclonal fractions of subacute sclerosing panenephalitis cere-brospinal fluid in africa with a specific immuneserum: first results (author's transl)]. | the oligoclonal gamma-fractions in the cerebrospinal fluid (c.s.f.) from one case of subacute sclerosing panencephalitis (s.s.p.e.) have been studied by means of immunolectrophoresis. the gamma-zone consisted of igg and free kappa and lambda chains. besides that, a rabbit immune serum against s.s.p.e. c.s.f. has been prepared. this immune serum did reveal in the gamma-zone one additional protein which is different from igg and normal tissue proteins. the possibility for this additionnal protein ... | 1977 | 64325 |
| peripheral blood b-lymphocyte abnormalities associated with hyperthyroidism of graves' disease. | proportions of peripheral blood thymus-derived t lymphocytes (t cells) and bone-marrow-derived b-lymphocytes (b cells) were studied in twelve hyperthyroid patients and ninety-nine non-hyperthyroid control subjects including thirty-nine healthy individuals and sixty patients with various disorders. all hyperthyroid patients had graves' disease and eight were untreated. the sheep erythrocyte (e)-rosetting technique was employed for enumeration of t cells and the immunofluorescent technique was use ... | 1976 | 64326 |
| [review: ontogeny of the oxygen transport in the blood]. | the biological importance of the oxygen affinity of the tetrameric haemoglobin molecule and its dependency from co-factors is described. oxygen affinity variations during development from embryonic to adult values are discussed in respect to oxygen uptake from maternal blood via placenta, in lungs and gills as well as to oxygen delivery into tissues. comparative aspects of the molecular mechanisms leading to affinity changes are presented. | 1976 | 64400 |
| detection of epstein-barr virus antigens with enzyme-conjugated antibody. | monospecific conjugated (fluorescein isothiocynate and horseradish peroxidase) goat antisera, prepared against three human immunoglobulin classes, im (mu), igg (gamma) and iga (alpha), were compared for their ability to detect human ig classes possessing specificity for epstein-barr virus (ebv) viral capsid antigens (vca) in a chronically infected human lymphoid cell line, p3j-hrik. it was determined that the enzyme system was significantly more sensitive than immunofluorescence in detecting mos ... | 1977 | 72045 |