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phylogeographical population structure of tiger quolls dasyurus maculatus (dasyuridae: marsupialia), an endangered carnivorous marsupial.tiger quolls, dasyurus maculatus, are the largest carnivorous marsupials still extant on the mainland of australia, and occupy an important ecological niche as top predators and scavengers. two allopatric subspecies are recognized, d.m. gracilis in north queensland, and d.m. maculatus in the southeast of the mainland and tasmania. d.m. gracilis is considered endangered while d.m. maculatus is listed as vulnerable to extinction; both subspecies are still in decline. phylogeographical subdivision ...199910583825
neurological symptoms 27 years after tiger bite. 199910472285
anatomical and electrophysiological evidence for gabaergic bipolar cells in tiger salamander retina.our previous work showed that about 12% of bipolar cells in salamander retina synthesize and take up gamma-aminobutyric acid (gaba), are gaba transporter (gat)-immunoreactive, and respond with a gat current to extracellularly applied gaba, suggesting that these bipolar cells use gaba, in addition to glutamate, as a neurotransmitter. further support for this idea was obtained in this study by use of immunogold electron microscopy and whole-cell patch clamp electrophysiology. ultrastructural analy ...199910746135
hypersensitivity pneumonitis in a worker exposed to tiger nut dust. 199910452779
effects of genetic structure of lupinus arboreus and previous herbivory on platyprepia virginalis caterpillars.two leaf-feeding caterpillars, western tussock moth (orgyia vetusta) and ranchman's tiger moth (platyprepia virginalis) are abundant on lupinus arboreus along the california coast. previous experiments and observations suggested that feeding caused by either of these two folivores could reduce the performance and possibly the abundance and distribution of the other species. previous common garden experiments also indicated that genetically determined characteristics of the host plants were impor ...199928308088
do images involuntarily trigger search? a test of pillsbury's hypothesis.pillsbury (1908) suggested that deciding to search for something in a scene consists of nothing more than forming a visual image of the target. if so, imaging should trigger search even when it would be more advantageous not to search. subjects were cued to form an image of a specified object (e.g., tiger) and to press a key when they had done so. this initiated the presentation of a sequence of pictures, with a single target digit interspersed; the subject's task was to report this digit. the s ...199912198782
anaesthesia of a sumatran tiger on eight occasions with ketamine, medetomidine and isoflurane.an 18-month-old male sumatran tiger was referred for endoscopy and dilatation of an oesophageal stricture. anaesthesia and bouginage was undertaken on eight occasions 2-3 weeks apart to dilate the oesophageal stricture.199916032083
materials science: new tigers in the fuel cell tank.after decades of incremental advances, a spurt of findings suggests that fuel cells that run on good old fossil fuels are almost ready for prime time. although conventional ceramic cells, known as solid oxide fuel cells, require expensive heat-resistant materials, a new generation of sofcs, including one featured on page 2031, converts hydrocarbons directly into electricity at lower temperatures. and a recent demonstration of a system of standard sofcs large enough to light up more than 200 home ...200017835104
amacrine cells of the anuran retina: morphology, chemical neuroanatomy, and physiology.amacrine cells are third-order retinal interneurons, projecting their processes into the inner plexiform layer. historically, they were not considered as neurons first. by the middle of the 20th century, their neuronal nature was confirmed, and their enormous diversity established. amacrine cells have been most successfully subdivided into morphological categories based on two parameters: diameter of the dendritic field and ramification pattern in the inner plexiform layer. works combining anato ...200010941173
fractionation and characterization of mushroom dietary fiber (nonstarch polysaccharides) as potential nutraceuticals from sclerotia of pleurotus tuber-regium (fries) singer.the nonstarch polysaccharides (nsps) in the total dietary fiber (tdf) from the sclerotia of pleurotus tuber-regium (tiger milk mushroom) were fractionated by the sequential use of chemical solvents. about half of the tdf was solubilized and two major alkali-soluble fractions (1 and 4 n sodium hydroxide) that contained 126 and 293 g/kg tdf were obtained. sugar analysis and infrared spectroscopy indicated that the nsps in these alkali-soluble fractions were mainly beta-glucans and chitin. these al ...200010956083
denoising of electron beam monte carlo dose distributions using digital filtering techniques.the monte carlo (mc) method has long been viewed as the ultimate dose distribution computational technique. the inherent stochastic dose fluctuations (i.e. noise), however, have several important disadvantages: noise will affect estimates of all the relevant dosimetric and radiobiological indices, and noise will degrade the resulting dose contour visualizations. we suggest the use of a post-processing denoising step to reduce statistical fluctuations and also improve dose contour visualization. ...200010943918
anoxia tolerance of con-familial tiger beetle larvae is associated with differences in energy flow and anaerobiosis.in this study, we compared survivorship, heat dissipation and biochemical features of anaerobiosis of two tiger beetle species (coleoptera: cicindelidae) exposed to anoxia. one species commonly experiences environmental immersion from rainfall and snowmelt (cicindela togata), and the habitat of the other (amblycheila cylindriformis) is not prone to flooding. the ancestral genus, a. cylindriformis, survives anoxia for only 2 days at 25 degrees c. in response to anoxia, these larvae immediately lo ...200010935521
effect of 11-cis 13-demethylretinal on phototransduction in bleach-adapted rod and cone photoreceptors.we used 11-cis 13-demethylretinal to examine the physiological consequences of retinal's noncovalent interaction with opsin in intact rod and cone photoreceptors during visual pigment regeneration. 11-cis 13-demethylretinal is an analog of 11-cis retinal in which the 13 position methyl group has been removed. biochemical experiments have shown that it is capable of binding in the chromophore pocket of opsin, forming a schiff-base linkage with the protein to produce a pigment, but at a much slowe ...200010919871
foraging under the risk of cannibalism leads to divergence in body size among tiger salamander larvae.populations of plants and animals are almost always made up of individuals of different sizes. in populations where cannibalism is common, this size variation can influence rates of mortality and growth and affect population regulation. size variation can be caused by a variety of mechanisms. one of these is due to size-specific responses to the threat of predation by potentially cannibalistic conspecifics. we investigated the role of anti-predator behavior in size structure development within s ...200028308182
depolarization-induced calcium channel facilitation in rod photoreceptors is independent of g proteins and phosphorylation.depolarization-induced facilitation of l-type ca channels in rod photoreceptors was investigated with nystatin-perforated and ruptured whole cell patch-clamp techniques in cells isolated from tiger salamander retina. induction of facilitation was voltage dependent with a half-maximal effect seen at prepulse potentials near +31 mv. reversal of facilitation was time dependent with fast (tau approximately 20 ms) and slow (tau approximately 1 s) components at -60 mv. incubation of cells with pertuss ...200010899191
temperatures in the cothill habitat of panaxia (callimorpha) dominula l. (the scarlet tiger moth).temperatures were monitored in precisely the places in cothill fen in which larvae of panaxia dominula pupate. at the critical time in june, the temperatures in the litter layer are diurnally often much higher than those used in previous constant temperature experiments. moreover, the daily mean temperature in the shaded litter layer is also higher than the shaded air temperature at 0.4 m, both temperatures being higher than the estimate of 12 degrees c used as baseline by others. the effects of ...200010849083
coping skills and tiger woods. 200011219381
caudal cervical disc protrusion in a bengal tiger (panthera tigris tigris).a young adult male white bengal tiger (panthera tigris tigris) presented with hindlimb ataxia. cervical and lumbar myelography revealed a compressive lesion of the cord at c(6-7). corticosteroid therapy and confinement failed to provide lasting remission of signs. a modified, inverted cone ventral slot decompression was used to remove the fibrous material causing cord compression at c(6-7). temporary horner's syndrome and laryngeal paresis developed postsurgically because of excessive tissue ret ...200011237151
[hallervorden-spatz disease: findings in the nigrostriatal system].hallervorden-spatz disease (hsd), a rare extrapyramidal motor illness, is usually only confirmed after death. in vivo diagnosis has relied hitherto on the combination of typical magnetic resonance imaging (mri) findings (the "eye of the tiger" sign) and heterogeneous clinical symptoms of movement disorder which have been regarded as almost pathognomonic. we report on the diagnostic contribution of 123j-beta-cit single photon emission computed tomography (spect) and 123j-ibzm spect in akinetic-ri ...200010996917
the eye-of-the-tiger sign. 200011110959
candidate gene analysis of thyroid hormone receptors in metamorphosing vs. nonmetamorphosing salamanders.we used two different experimental approaches to test the hypothesis that thyroid hormone receptor (tr) variation is associated with alternate life cycles modes in ambystomatid salamanders. in the first experiment, the inheritance of tralpha and trbeta genotypes was determined for metamorphic and non metamorphic offspring from backcrosses between ambystoma mexicanum (an obligate metamorphic-failure species) and metamorphic f1 hybrids (a. mexicanum x a. tigrinum tigrinum). the segregation of tr g ...200011012711
intrafamilial phenotypic variability of hallervorden-spätz syndrome in a tunisian family.we report clinical features, ct-scan and mri findings of three siblings with hallervorden-spätz disease, one of them followed for more than 20years. patient 1 presented at age 10 with progressive generalized dystonia. five years later she had violent dystonic spasms with opisthotonos and marked oro-mandibular involvement. later, dystonia stabilized and examination showed dysarthria, multiple deformities related to dystonic posturing, retinal degeneration and no cognitive impairment. cerebral ct- ...200010817958
i4aa-sensitive chloride current contributes to the center light responses of bipolar cells in the tiger salamander retina.light-evoked currents in depolarizing and hyperpolarizing bipolar cells (dbcs and hbcs) were recorded under voltage-clamp conditions in living retinal slices of the larval tiger salamander. responses to illumination at the center of the dbcs' and hbcs' receptive fields were mediated by two postsynaptic currents: deltai(c), a glutamate-gated cation current with a reversal potential near 0 mv, and deltai(cl), a chloride current with a reversal potential near -60 mv. in dbcs deltai(c) was suppresse ...200010848563
the molecular basis of melanism and mimicry in a swallowtail butterfly.melanism in lepidoptera, either industrial or in mimicry, is one of the most commonly cited examples of natural selection [1] [2]. despite extensive studies of the frequency and maintenance of melanic genes in insect populations [1] [2], there has been little work on the underlying molecular mechanisms. nowhere is butterfly melanism more striking than in the eastern tiger swallowtail (papilio glaucus) of north america [3] [4] [5]. in this species, females can be either yellow (wild type) or blac ...200010837223
triggering of cryoprotectant synthesis in the woolly bear caterpillar (pyrrharctia isabella lepidoptera: arctiidae).isabella tiger moths (pyrrharctia isabella) overwinter as caterpillars (i.e., woolly bears) that can survive freezing at moderate subzero temperatures. we observed an increase in hemolymph osmolality for field-collected woolly bears during october (325 +/- 47 to 445 +/- 27 mosmol/liter) and tested the influence of temperature and moisture levels on cryoprotectant production. laboratory acclimation was done at 5 degrees c in moist conditions and at 25 degrees c acclimation in both dry and moist c ...200010684559
fraction of the dark current carried by ca(2+) through cgmp-gated ion channels of intact rod and cone photoreceptors.the selectivity for ca(2+) over na(+), pca/pna, is higher in cgmp-gated (cng) ion channels of retinal cone photoreceptors than in those of rods. to ascertain the physiological significance of this fact, we determined the fraction of the cyclic nucleotide-gated current specifically carried by ca(2+) in intact rods and cones. we activated cng channels by suddenly (<5 ms) increasing free 8br-cgmp in the cytoplasm of rods or cones loaded with a caged ester of the cyclic nucleotide. simultaneous with ...200011099344
competition between the gypsy moth, lymantria dispar, and the northern tiger swallowtail, papilio canadensis: interactions mediated by host plant chemistry, pathogens, and parasitoids.the gypsy moth, lymantria dispar, and the northern tiger swallowtail, papilio canadensis, overlap geographically as well as in their host ranges. adult female swallowtails are incapable of distinguishing between damaged and undamaged leaves, and the opportunities for competition between these two species are numerous. we designed field and laboratory experiments to look for evidence of indirect competition between p. canadensis and l. dispar larvae. swallowtail caterpillars were reared in the la ...200024595833
three levels of lateral inhibition: a space-time study of the retina of the tiger salamander.the space-time patterns of activity generated across arrays of retinal neurons can provide a sensitive measurement of the effects of neural interactions underlying retinal activity. we measured the excitatory and inhibitory components associated with these patterns at each cellular level in the retina and further dissected inhibitory components pharmacologically. using perforated and loose patch recording, we measured the voltages, currents, or spiking at 91 lateral positions covering approximat ...200010684895
developmental study of the shortened spinal cord in the adult tiger puffer fish, takifugu rubripes (teleostei).abstract the spinal cords of vertebrates are generally divided into the cord proper and the minute filum terminale. while the spinal cord extends the entire length of the vertebral canal in the adult tiger puffer, takifugu rubripes, the cord proper is greatly reduced in length and almost all of the canal is occupied by the filum terminale, which is tape-like rather than thread-like. the dorsal and ventral roots of the spinal nerves extend, respectively, above and below the filum terminale; as a ...200010723077
how slippage-derived sequences are incorporated into rrna variable-region secondary structure: implications for phylogeny reconstruction.we analyzed the type and frequency of mutational changes in hypervariable rrna regions, using the highly length-variable region v4 of the small subunit rrna locus of tiger beetles (cicindelidae) as an example. phylogenetic analysis of indels in closely related species showed that (1) most indels are single nucleotides (usually a or t and sometimes g) or di-nucleotides of a and t. these occur at numerous foci, and they exhibit a strong bias for duplication of 5' single and di-nucleotide motifs bu ...200010712842
effects of betaxolol on light responses and membrane conductance in retinal ganglion cells.to examine the physiological effects of betaxolol, a beta1-adrenergic receptor blocker commonly used in the treatment of glaucoma, on retinal ganglion cells and to evaluate its potential to elicit responses consistent with a neuroprotective agent against ganglion cell degeneration.200010711687
gaba(c) receptors control adaptive changes in a glycinergic inhibitory pathway in salamander retina.we studied the role of gaba in adaptive changes in a lateral inhibitory system in the tiger salamander retina. in dark-adapted retinal slice preparations picrotoxin caused a slow enhancement of glycine-mediated ipscs in ganglion cells. the enhancement of glycinergic ipscs developed slowly over the course of 5-20 min, even though picrotoxin blocked both gaba(a) and gaba(c) receptors within a few seconds. the slow enhancement of glycinergic ipscs by picrotoxin was much weaker in light-adapted prep ...200010632610
dosimetry characterization of 32p catheter-based vascular brachytherapy source wire.dosimetry measurements and monte carlo simulations for a catheter-based 32p endovascular brachytherapy source wire are described. the measured dose rates were obtained using both radiochromic dye film and an automated plastic scintillator. the investigated source has dimensions of 27 mm in length and 0.24 mm in diameter, and is encapsulated in niti. for the radiochromic film measurements, calibrated radiochromic dye film was irradiated at distances between 1 and 5 mm from the source axis in a-15 ...200010984223
misfolding of membrane proteins in health and disease: the lady or the tiger?protein misfolding is increasingly recognized as a factor in many diseases, including cystic fibrosis, parkinson's, alzheimer's and atherosclerosis. many proteins involved in misfolding-based pathologies are membrane-associated, such that the bilayer may play roles in normal and aberrant folding. it can be argued that the in vivo partitioning of eukaryotic membrane proteins between folding and misfolding pathways is under kinetic control. moreover, the balance between these pathways can be surpr ...200010981632
dihydropyridine-sensitive calcium currents in bipolar cells of salamander retina are inhibited by reductions in extracellular chloride.dihydropyridine-sensitive calcium currents (i(ca)) in photoreceptors are unusual in that they can be inhibited by reductions in extracellular chloride. the present study examined whether i(ca) in retinal bipolar cells, which as in photoreceptors mediates sustained neurotransmission, is also inhibited by reductions in chloride. nystatin-perforated patch, whole cell recordings were obtained from bipolar cells in a retinal slice preparation of larval tiger salamander. in the presence of ba(2+), vol ...200010675784
the national practitioner data bank: big brother or paper tiger? 200010121442
fate and the biochemical effects of 2,4,6-trinitrotoluene exposure to tiger salamanders (ambystoma tigrinum).biotransformation, metabolic enzyme profiles, and the glutathione antioxidant system in tiger salamanders (ambystoma tigrinum) from a 14-day exposure to 2,4,6-trinitrotoluene (tnt) in situ were examined. concentrations of parent compound and metabolites were measured in skin, kidney, and liver tissue. in addition, cytochrome p450 (p450) and cytochrome b5 content and their dependent isozyme activities, ethoxyresorufin o-dealkylation, pentoxyresorufin o-dealkylation, and the glutathione antioxidan ...200010831332
mechanics of lung ventilation in a post-metamorphic salamander, ambystoma tigrinum.the mechanics of lung ventilation in frogs and aquatic salamanders has been well characterized, whereas lung ventilation in terrestrial-phase (post-metamorphic) salamanders has received little attention. we used electromyography (emg), x-ray videography, standard videography and buccal and body cavity pressure measurements to characterize the ventilation mechanics of adult (post-metamorphic) tiger salamanders (ambystoma tigrinum). three results emerged: (i) under terrestrial conditions or when f ...200010683167
relationships between odor-elicited oscillations in the salamander olfactory epithelium and olfactory bulb.oscillations in neuronal population activity, or the synchronous neuronal spiking that underlies them, are thought to play a functional role in sensory processing in the cns. in the olfactory system, stimulus-induced oscillations are observed both in central processing areas and in the peripheral receptor epithelium. to examine the relationship between these peripheral and central oscillations, we recorded local field potentials simultaneously from the olfactory epithelium and olfactory bulb in ...200010669491
health and rapid economic change in the late twentieth century.rapidly expanding economies, such as the post-war tiger economies, are associated with increasing health and rapidly contracting economies, such as central and eastern europe in the early 1990s, are associated with declining health. in central and eastern europe health decline in association with economic contraction has been mediated by changes in income distribution and, also, by health-determining aspects of civil society. the nations of central and eastern europe are an example of swift econ ...200010972426
differential modulation of rod and cone calcium currents in tiger salamander retina by d2 dopamine receptors and camp.synaptic transmission from vertebrate photoreceptors involves activation of l-type calcium currents (ica). dopamine is an important circadian neuromodulator in the retina and photoreceptors possess d2 dopamine receptors. we examined modulation of ica by dopamine and camp in retinal slices and isolated cells of larval tiger salamander. results show that dopamine and a d2 agonist, quinpirole, enhanced ica in rods and red-, blue- and uv-sensitive small single cones but inhibited ica in red-sensitiv ...200011029623
polymicrobial tenosynovitis with pasteurella multocida and other gram negative bacilli after a siberian tiger bite.mammalian bites present a considerable clinical problem because they are often associated with bacterial infections. pasteurella multocida is a microorganism that commonly infects both canine and small feline bites. zoonotic infections developing after large feline bites have been recognised, although their reports are limited. we describe a 35 year old man who was bitten by a siberian tiger and who developed infectious tenosynovitis secondary to p multocida, bergeyella (weeksella) zoohelcum, an ...200011127273
spontaneous discrimination of natural stimuli by chimpanzees (pan troglodytes).six chimpanzees (pan troglodytes) were presented with pairs of color photographic images of 5 different categories of animals (cat, chimp, gorilla, tiger, fish). the subjects responded to each pair using symbols for "same" and "different." both within- and between-category discriminations were tested, and all chimpanzees classified the image pairs in accordance with the 5 experimenter-defined categories under conditions of nondifferential reinforcement. although previous studies have demonstrate ...200011149543
birth of siberian tiger (panthera tigris altaica) cubs after transvaginal artificial insemination.a captive 9-yr-old female siberian tiger (panthera tigris altaica) at the lisbon zoo was treated with equine chorionic gonadotropin followed 80 hr later by human chorionic gonadotropin to stimulate follicular development and ovulation, respectively. forty-one hours after the second hormone administration, the tiger was subjected to transvaginal artificial insemination with diluted fresh semen containing 500 x 10(6) motile spermatozoa obtained from an 8-yr-old male siberian tiger via electroejacu ...200011428407
microsatellite polymorphism and the population structure of the black tiger shrimp (penaeus monodon) in thailand.genetic variation and differentiation of thai penaeus monodon from five geographic locations (chumphon, trad, phangnga, satun, and trang) were investigated using five microsatellite loci (cupmo18, di25, di27, cscupmo1, and cscupmo2). the number of alleles across the five loci ranged from 19 to 30, and heterozygosities ranged from 0.49 to 0.95. the mean number of alleles and effective number of alleles per locus were 21.0 to 26.6 and 13.1 to 20.4, respectively. the average heterozygosity across a ...200010960123
helicobacter acinonychis eradication leading to the resolution of gastric lesions in tigers. 200010975334
immunomodulation by dietary beta-1, 3-glucan in the brooders of the black tiger shrimp penaeus monodon.the present study evaluated the effectiveness of beta-1,3-glucan derived from schizophyllum commune in enhancing shrimp survival as well as haemocyte phagocytosis and superoxide anion production in brooder penaeus monodon. pond-reared p. monodon adults (135 +/- 25 g) stocked in outdoor or indoor tanks were fed either a test diet containing beta-1,3-glucan (2.0 g kg(-1) or a glucan-free control diet for 40 days. their survival was compared. the brooders reared in indoor tanks were analysed at day ...200011016585
clinical toxicology: a tropical australian perspective.tropical australia has an amazing diversity of venomous fauna, from "the world's most venomous creature," the multi-tentacled (chirodropid) box jellyfish chironex fleckeri, to aggressive spiders whose venom remains to be characterized. all genera of highly venomous australasian elapid snakes are present, except for tiger snakes. most notable is the taipan (oxyuranus scutellatus), with the most efficient "snap-release" biting mechanism of any snake and venom components causing the full constellat ...200010688264
characterisation of different morphological features of black tiger shrimp (penaeus monodon) haemocytes using monoclonal antibodies.monoclonal antibodies (mabs) specific for penaeus monodon haemocytes were produced by immunising mice with membrane lysates of shrimp haemocytes. four mabs (wsh 6, wsh 7, wsh 8 and wsh 16) were characterised using flow cytometry, light microscopy, laser scanning microscopy, electron microscopy and immunoprecipitation. wsh 6 recognised a carbohydrate determinant on an 85 kda molecule. wsh 7, wsh 8 and wsh 16 recognised 50, 35 and 115 kda molecules, respectively. for all mabs, differences in amoun ...200011016586
degenerative spinal disease in large felids.degenerative spinal disorders, including intervertebral disc disease and spondylosis, seldom occur in domestic cats. in contrast, a retrospective study of 13 lions (panthera leo), 16 tigers (panthera tigris), 4 leopards (panthera pardis), 1 snow leopard (panthera uncia), and 3 jaguars (panthera onca) from the knoxville zoo that died or were euthanatized from 1976 to 1996 indicated that degenerative spinal disease is an important problem in large nondomestic felids. the medical record, radiograph ...200010884118
the envenomation syndrome caused by the australian red-bellied black snake pseudechis porphyriacus.the australian elapids inject venom which is characteristic of each species; and which cause characteristic and specific envenomation syndromes in human victims of snakebite. because many of the medically significant australian elapids look similar, when glimpsed in the field by snakebite victims, defining human envenomation syndromes with secure species identification has been a slow process. correlations between securely identified species and the human envenomation syndromes which they produc ...200010858512
the complete sequence of the mitochondrial genome of the crustacean penaeus monodon: are malacostracan crustaceans more closely related to insects than to branchiopods?the complete sequence of the mitochondrial genome of the giant tiger prawn, penaeus monodon (arthropoda, crustacea, malacostraca), is presented. the gene content and gene order are identical to those observed in drosophila yakuba. the overall at composition is lower than that observed in the known insect mitochondrial genomes, but higher than that observed in the other two crustaceans for which complete mitochondrial sequence is available. analysis of the effect of nucleotide bias on codon compo ...200010833192
recovery of penaeus monodon from functional anaemia after exposure to sublethal concentration of nitrite at different ph levels.tiger shrimp penaeus monodon (14.79+/-0.19 g) which had been exposed individually to 0.72 mm nitrite at ph 6.8, 8.2 and 9.8 after 3, 6, 12, 24 and 48 h were examined for the water nitrite concentration, hemolymph nitrite, oxyhemocyanin, protein and osmolality levels. same parameters were examined for shrimp depurated in nitrite-free water after 3, 6, 12 and 24 h following 48 h exposure to nitrite. in the nitrite-exposed test, nitrite influx, hemolymph nitrite and osmotic differential (medium osm ...200010930651
pharmacological adrenalectomy with mitotane.the potential of mitotane (ortho, para'-ddd, commonly used to treat adrenal carcinomas in humans and dogs) was investigated as an alternative to surgical adrenalectomy in birds, salamanders, and lizards. house sparrows (passer domesticus) were injected twice daily with vehicle or one of two doses of mitotane (225 or 450 mg/kg), and basal and stress-induced levels of corticosterone (cort) were measured 3 and 5 days after injections. mitotane reduced basal cort levels to nondetectable and abolishe ...200011042008
[origin of lepidoptera fauna of the southern transural region].the butterfly fauna of the southern transural region began mainly through the migration of insects from the urals and kazakhstan, since the end of the cretaceous period to the end of paleogen, the transural region was covered by an epiplatform sea. as this sea was retreating, the first regions of dry land appeared, which had boundaries with kazakhstan and the urals. they were the first to be populated by lepidoptera. during the pleocene and then after the pleistocene cooling events, insects sett ...200011042964
[medical treatment of dystocia in zoo animals].kasuistics of zoo animals with dystocia (anoa, giraffe, snow goat, scimitar horned antelope, bactrian camel, tiger, diana monkey) treated with special pharmaceuticals (glukokortikoids, cienbuterol, denaverine, carbetocin) and distance injections by blow darts are introduced. these procedures are described as very effective and save methods of obstetrics especially in zoo animals.200011155524
introducing foreign dna into tiger shrimp (penaeus monodon) by electroporation.electroporation was used to introduce pflag-cmv-1-bap, a dna fragment that includes a bacterial alkaline phosphatase gene driven by a human cytomegalovirus (cmv) promoter, into penaeus monodon zygotes. the transgenic tiger shrimp was achievedby using 10kv, 28 pulses, 120 g sec pulse time, 10 cycles, and a dna concentration of 37.5 microg/ml. the hatching rate of electroporated zygotes (46%) was significantly lower than that of zygotes in the untreated group (89%). the survival rate of postlarvae ...200011191866
genetic characterization of feline parvovirus sequences from various carnivores.infections with viruses of the feline parvovirus subgroup such as feline panleukopenia virus (fpv), mink enteritis virus (mev) and canine parvovirus (cpv-2) [together with its new antigenic types (cpv-2a, cpv-2b)] have been reported from several wild carnivore species. to examine the susceptibility of different species to the various parvoviruses and their antigenic types, samples from wild carnivores with acute parvovirus infections were collected. viral dna was amplified, and subsequently anal ...200010644832
cloning and functional characterization of salamander rod and cone arrestins.to clone, localize, and determine functional binding characteristics of rod and cone arrestins from the retina of the tiger salamander (ambystoma tigrinum).200010937552
sulfakinin neuropeptides in a crustacean. isolation, identification andtissue localization in the tiger prawn penaeus monodon.the sulfakinin (sk) family of neuropeptides are characterized by a c-terminal octapeptide sequence that begins with two acidic residues (most commonly dd), and ends with yghmrf-nh2, usually with the tyrosyl residue sulfated. so far, sulfakinins have only been identified in insects and the present study was initiated to investigate if the family is more widely distributed within the arthropods. purification of an extract of the central nervous system of the giant tiger prawn penaeus monodon has r ...200010672025
the neurotoxicity of the venom phospholipases a(2), notexin and taipoxin.the presynaptically active, toxic phospholipases known as notexin and taipoxin are principal components of the venom of the australian tiger snake and the australian taipan respectively. the inoculation of the toxins into one hind limb of rats caused, within 1 h, the depletion of transmitter from the motor nerve terminals of the soleus muscle. this was followed by the degeneration of the motor nerve terminals and of the axonal cytoskeleton. by 24 h 70% of muscle fibers were completely denervated ...200010686073
subcutaneous atypical mycobacteriosis in captive tiger quolls (dasyurus maculatus).from july 1989 to october 1998, 9/37 (24%) adult captive tiger quolls (dasyurus maculatus) were diagnosed with atypical mycobacterial infection involving the subcutis and skin. females were more often affected than males (seven females, two males). grossly, lesions presented as focal thickenings, plaques, and abscesses within the subcutis, often with fistulous tracts. the subcutis and skin overlying cervical and thoracic regions were the primary sites of infection. cytology of subcutaneous impre ...200010714642
identification and purification of vitamin k-dependent proteins and peptides with monoclonal antibodies specific for gamma -carboxyglutamyl (gla) residues.novel monoclonal antibodies that specifically recognize gamma-carboxyglutamyl (gla) residues in proteins and peptides have been produced. as demonstrated by western blot and time-resolved immunofluorescence assays the antibodies are pan-specific for most or all of the gla-containing proteins tested (factors vii, ix, and x, prothrombin, protein c, protein s, growth arrest-specific protein 6, bone gla protein, conantokin g from a cone snail, and factor xa-like proteins from snake venom). only the ...200010779512
diagnosis of penaeus monodon-type baculovirus by pcr and by elisa of occlusion bodies.the black tiger prawn penaeus monodon is a valuable aquaculture product in taiwan. two specific diagnostic methods were established for p. monodon-type baculovirus, one using polymerase chain reaction (pcr) technology and the other enzyme-linked immunosorbent assay (elisa) technology. monodon-type baculovirus (mbv) was purified by sucrose gradient centrifugation from occlusion bodies of mbv-infected postlarvae of p. monodon. mbv dna was subsequently purified from the occlusion bodies and its pre ...200010782342
embryo technology in conservation efforts for endangered felids.most of the 36 species of wild cats are classified as threatened, vulnerable or endangered due to poaching and habitat loss. the important role of assisted reproduction techniques (art) as part of a multifaceted captive breeding program for selected wild cat species is gradually gaining acceptance. this recognition is a result of the progress made during the last decade in which the feasibility of oocyte recovery from gonadotropin-treated females, in vitro fertilization, embryo cryopreservation ...200010735071
lethal attribute of serine protease secreted by vibrio alginolyticus strains in kuruma prawn penaeus japonicus.toxicity of the extracellular products (ecp) and the lethal attribute of serine protease secreted by five pathogenic vibrio alginolyticus strains from various sources in kuruma prawn penaeus japonicus were studied. the ecps of organisms originally isolated from diseased kuruma prawn or small abalone haliotis diversicolor supertexta were more lethal (ld50 value of 0.48 or 0.41 microg protein/g prawn) than those from diseased tiger prawn p. monodon, yellowfin porgy acanthopagrus latus or horse mac ...200010739107
inhibition of luminescence and virulence in the black tiger prawn (penaeus monodon) pathogen vibrio harveyi by intercellular signal antagonists.expression of luminescence in the penaeus monodon pathogen vibrio harveyi is regulated by an intercellular quorum sensing mechanism involving the synthesis and detection of two signaling molecules, one of which is n-hydroxy butanoyl-l-homoserine lactone and the other of which is uncharacterized. indirect evidence has suggested that virulence, associated with a toxic extracellular protein, and luminescence in v. harveyi are coregulated. in this study the effects of an acylated homoserine lactone ...200010788385
study on the free amino acid levels in the hemolymph, gill, hepatopancreas and muscle of penaeus monodon exposed to elevated ambient ammonia.tiger shrimp penaeus monodon following 24 h exposure to 0.002 (control), 0.072 and 0.718 mm ammonia were examined for the free amino acid (faa), ammonia and urea levels in the hemolymph, gill, hepatopancreas and muscle. control shrimps contained total faa in hemolymph (1.19 µmol ml(-1)), gill (21.81 µmol g(-1)), hepatopancreas (100.81 µmol g(-1)) and muscle (239.54 µmol g(-1)). glycine and arginine were the major contributors to the total faa pool, and made up of 90% of the total faa in the musc ...200010930648
the penaeus monodon chitinase 1 gene is differentially expressed in the hepatopancreas during the molt cycle.we have isolated a full-length chitinase complementary dna from the tiger shrimp penaeus monodon that encodes a 621 amino acid protein possessing the functional domains of the chitinase protein family. the penaeus monodon chitinase 1 (pmchi-1) gene product is 81.8% identical to a chitinase 1 protein expressed in the hepatopancreas of penaeus japonicus. analysis by reverse transcription-polymerase chain reaction (rt-pcr) indicates that pmchi-1 messenger rna is detectable in the hepatopancreas and ...200010811951
genomic ancestry of the american puma (puma concolor).puma concolor, a large american cat species, occupies the most extensive range of any new world terrestrial mammal, spanning 110 degrees of latitude from the canadian yukon to the straits of magellan. until the recent holocene, pumas coexisted with a diverse array of carnivores including the american lion (panthera atrox), the north american cheetah (miracynonyx trumani), and the saber toothed tiger (smilodon fatalis). genomic dna specimens from 315 pumas of specified geographic origin (261 cont ...200010833043
suppressive actions of betaxolol on ionic currents in retinal ganglion cells may explain its neuroprotective effects.betaxolol, a beta 1-selective adrenoceptor antagonist, is widely used in the treatment of glaucoma. in addition to its ocular hypotensive effects, betaxolol has been suggested to act as a retinal neuroprotective agent (osborne et al., 1997). to investigate possible mechanisms underlying the neuroprotective effects, we tested the actions of betaxolol on ion channels and calcium signaling in isolated retinal ganglion cells. betaxolol (50 microm) reduced by about 20% the high-voltage-activated (hva ...200010870519
a new bacterial white spot syndrome (bwss) in cultured tiger shrimp penaeus monodon and its comparison with white spot syndrome (wss) caused by virus.this paper describes a new bacterial white spot syndrome (bwss) in cultured tiger shrimp penaeus monodon. the affected shrimp showed white spots similar to those caused by white spot syndrome virus (wssv), but the shrimp remained active and grew normally without significant mortalities. the study revealed no evidence of wssv infection using electron microscopy, histopathology and nested polymerase chain reaction. electron microscopy indicated bacteria associated with white spot formation, and wi ...200010907134
five crustacean hyperglycemic family hormones of penaeus monodon: complementary dna sequence and identification in single sinus glands by electrospray ionization-fourier transform mass spectrometry.five novel neuropeptides, designated pm-sgp-i to -v, of the crustacean hyperglycemic hormone (chh) family have been identified from the giant tiger prawn penaeus monodon by isolation of the preprohormone genes from an eyestalk complementary dna library. on the basis of sequence similarity, the encoded peptides have been classified as chh-like type i hormones, which include all known chhs and the molt-inhibiting hormone (mih) of the lobster homarus americanus. consistent with chh type i preprohor ...200010804243
use of prawn blood agar hemolysis to screen for bacteria pathogenic to cultured tiger prawns penaeus monodon.a newly developed prawn blood agar consisting of 1 ml of tiger prawn hemolymph in medium containing 200 ppm rose bengal was used to determine the hemolytic activity of 35 isolates of bacteria obtained from cultured tiger prawns penaeus monodon and their rearing water. for comparison, the hemolytic activity of these isolates was also determined in sheep blood agar. nine isolates (25.7% of total) showed different hemolytic reactions on prawn blood agar and sheep blood agar. from the 35 isolates, 8 ...200011145455
relating middle-ear acoustic performance to body size in the cat family: measurements and models.is the acoustic performance of the mammalian middle ear dependent on body size? we focus on the cat family, because of its qualitatively uniform (and distinctive) middle-ear structure, large size range, and the extensive data available from domestic cats which provide a framework for relating middle-ear acoustics to structure. we report measurements of acoustic admittance in 17 live adult ears of 11 exotic species, ranging in size from sand cat (3 kg) to tiger (180 kg). for low frequencies, the ...200010879948
gastric ollulanus tricuspis infection identified in captive cheetahs (acinonyx jubatus) with chronic vomiting.gastritis, vomition and weight loss are common in captive cheetahs (acinonyx jubatus). gastric spiral bacteria (helicobacter spp.) and the very small, viviparous nematode ollulanus tricuspis, a stomach worm of cats, are believed to be important causes. three sibling cheetahs at wellington zoo, new zealand, developed chronic vomiting, diarrhoea and debility. their parents were both south african-born. response to antibacterial treatment was poor. endoscopic examinations revealed chronic lymphopla ...200011212940
hallervorden-spatz syndrome in two siblings diagnosed by clinical features and magnetic resonance imaging.the hallervorden-spatz syndrome (hss) is a rare condition characterized by extrapyramidal and pyramidal signs, dystonia, dysarthria, retinal degeneration, dementia and a progressive course. the development of magnetic resonance imaging (mri) has increased the number of clinical and pathological reports of hss. mri pallidal abnormalities are called "eye of the tiger" signs. the combination of clinical features and mri findings can be considered as highly suggestive of a diagnosis of hss. patient ...200011253896
shrimp culture in thailand: environmental impacts and social responses.black tiger shrimp (penaeus monodon) is a major aquaculture commodity among southeast asian producers and remains a popular food export world-wide. food brokers in japan and the united states purchase huge quantities of these farmed shrimp and return significant foreign exchange earnings to developing nations like thailand, a major producer and exporter since the early 1990s. however, coastal areas cannot sustain intensive shrimp farm production and local growers often end up in debt. can the ne ...200017208685
cloning of the fish cell line ssn-1 for piscine nodaviruses.six cell clones were derived from the ssn-1 cell line, which is composed of a mixed cell population and persistently infected with a c-type retrovirus (snrv). these clones were susceptible to 4 piscine nodavirus strains belonging to different genotypes (sjnnv, rgnnv, tpnnv and *bfnnv [striped jack, redspotted grouper, tiger puffer and barfin flounder nervous necrosis viruses]). three clones, designated a-6, e-9, and e-11, were highly permissive to nodavirus infection and production. the virus-in ...200011145456
[desulfacinum subterraneum sp.nov.--a new thermophilic sulfate-reducing bacterium isolated from a high temperature oil field ].a new thermophilic sulfate-reducing bacterium isolated from the high-temperature white tiger oil field (vietnam) is described. cells of the bacterium are oval (0.4-0.6 by 0.6-1.8 microns), nonmotile, non-spore-forming, and gram-negative. growth occurs at 45 to 65 degrees c (with an optimum at 60 degrees c) at nacl concentrations of 0 to 50 g/l. in the course of sulfate reduction, the organism can utilize lactate, pyruvate, malate, fumarate, ethanol, salts of fatty acids (formate, acetate, propio ...200111558281
globalization and health: results and options.the last two decades have witnessed the emergence and consolidation of an economic paradigm which emphasizes domestic deregulation and the removal of barriers to international trade and finance. if properly managed, such an approach can lead to perceptible gains in health status. where markets are non-exclusionary, regulatory institutions strong and safety nets in place, globalization enhances the performance of countries with a good human and physical infrastructure but narrow domestic markets. ...200111584731
two individuals with features of both xeroderma pigmentosum and trichothiodystrophy highlight the complexity of the clinical outcomes of mutations in the xpd gene.the xeroderma pigmentosum group d (xpd) protein is a subunit of transcription factor tfiih with dna helicase activity. tfiih has two functions, in basal transcription and nucleotide excision repair. mutations in xpd that affect dna repair but not transcription result in the skin cancer-prone disorder, xeroderma pigmentosum (xp). if transcription is also affected, the result is the multi-system disorder trichothiodystrophy (ttd), in which there is no skin cancer predisposition, or in rare cases, ...200111709541
vascular endothelial growth factor-induced angiogenesis: crouching tiger or hidden dragon? 200111419899
adaptation to temporal contrast in primate and salamander retina.visual adaptation to temporal contrast (intensity modulation of a spatially uniform, randomly flickering stimulus) was examined in simultaneously recorded ensembles of retinal ganglion cells (rgcs) in tiger salamander and macaque monkey retina. slow contrast adaptation similar to that recently discovered in salamander and rabbit retina was observed in monkey retina. a novel method was developed to quantify the effect of temporal contrast on steady-state sensitivity and kinetics of light response ...200111739598
a non-stop, single-tube, semi-nested pcr technique for grading the severity of white spot syndrome virus infections in penaeus monodon.a single-tube, non-stop, semi-nested polymerase chain reaction (pcr) technique was developed for simultaneous detection and severity grading of white spot syndrome virus (wssv) infections in the black tiger shrimp penaeus monodon. the test uses 1 sense primer and 3 antisense primers that produce up to 3 pcr products (1100, 526 and 250 base pairs [bp]) depending upon the severity of infection. specifically, heavy infections (> or = 2 x 10(4) viral particles) of wssv produce all 3 fragments, while ...200111804423
transmission of the ambystoma tigrinum virus to alternative hosts.ambystoma tigrinum virus (atv) is a lethal virus originally isolated from sonora tiger salamanders ambystoma tigrinum stebbinsi in the san rafael valley in southern arizona. usa. atv is implicated in several salamander epizootics. we attempted to transmit atv experimentally to fish and amphibians by injection, water bath exposure, or feeding to test whether atv can cause clinical signs of infection or be recovered from exposed individuals that do not show clinical signs. cell culture and polymer ...200111710549
megaoesophagus in adult dogs secondary to australian tiger snake envenomation.four cases of megaoesophagus secondary to tiger snake envenomation are reported. history in all cases suggested megaoesophagus was not present prior to snake envenomation. diagnosis of megaoesophagus was confirmed by thoracic radiography in all cases. one dog died of respiratory failure. the remaining three dogs recovered, with gradual resolution of clinical signs associated with megaoesophagus.200111712704
genetic manipulations in aquaculture: a review of stock improvement by classical and modern technologies.the aim of this review was to highlight the extent to which the genetic technologies are implemented by the aquaculture industry. the review shows that some of the modern genetic technologies are already extensively applied by the diverse aquaculture industries, though not to the same extent for all important aquacultured species (according to fao 1998 figures). some species (common carp, atlantic salmon, rainbow trout, channel catfish, nile tilapia, and the pacific oyster) received concentrated ...200111841164
toxoplasma gondii antibodies in exotic wild felids from brazilian zoos.serum samples from 37 captive exotic felids in 12 zoos from six brazilian states were assayed for antibodies to toxoplasma gondii by the modified agglutination test using formalin-fixed whole tachyzoites. titers greater than or equal to 1:20 were considered positive. antibodies to t. gondii were found in 24 of 37 (64.9%) felids, including one european lynx (lynx lynx), two jungle cats (felis chaus), two servals (leptailurus serval), two tigers (panthera tigris), three leopards (panthera pardus), ...200112785684
infectivity of aquabirnavirus strains to various marine fish species.to determine the infectivity of marine birnavirus (mabv) in various marine fish species, experimental infection was performed in combination groups of 5 fish species with 7 strains of mabv and 1 strain of infectious pancreatic necrosis virus (ipnv). mortality was observed in yellowtail seriola quinqueradiata and amberjack s. dumerili infected with mabv strains y-6, y-10k and h-1, but not in other infected species. mabv was reisolated from most combination groups, but the virus isolation rate and ...200111678225
irradiation of diets fed to captive exotic felids: microbial destruction, consumption, and fecal consistency.two frozen, raw horse meat-based diets fed to captive exotic felids at brookfield zoo were irradiated to determine the extent of microbial destruction and whether radiation treatment would affect consumption and/or fecal consistency in exotic cats. fifteen cats, two african lions (panthera leo), two amur tigers (panthera tigris altaica), one amur leopard (panthera pardus orientalis), two clouded leopards (neofelis nebulosa), two caracals (felis caracal), one bobcat (felis rufus), and five fishin ...200112785680
development of vitellogenin-elisa, an in vivo bioassay, and identification of two vitellogenesis-inhibiting hormones of the tiger shrimp penaeus monodon.a sandwich enzyme-linked immunosorbent assay (elisa) for the quantification of vitellogenin in the hemolymph of penaeus monodon is reported. lipovitellin from the mature ovary was purified using hydroxylapatite column chromatography and used as the standard protein, which was serially diluted from 3336 to 6.51 microg and gave a linear plot. sensitivity results showed elisa was insensitive to samples that did not contain vitellogenin or lipovitellin. specificity results showed the degree of the d ...200114961329
genetic heterogeneity of the giant tiger shrimp (penaeus monodon) in thailand revealed by rapd and mitochondrial dna rflp analyses.genetic diversity of the giant tiger shrimp (penaeus monodon) collected from 5 areas, chumphon and trat (gulf of thailand), and phangnga, satun, and trang (andaman sea), was examined by randomly amplified polymorphic dna (rapd) and mitochondrial dna (16s ribosomal dna and an intergenic coi-coii) polymorphism. a total of 53 polymorphic fragments from ubc299, ubc273, and ubc268 was consistently scored across all samples. from the respective primers 26, 32, and 30 genotypes were generated. a 260-bp ...200114961335
lingering doubts about spongiform encephalopathy and creutzfeldt-jakob disease.bovine spongiform encephalopathy (bse) is an infectious disease and has been transmitted orally to many other animals, including humans. there is clear evidence of maternal transmission, although disagreement on the source of the bse agent remains. the current theories link the origin of bse to common scrapie in sheep. twenty different strains of the scrapie agent have been isolated from sheep. a search of the literature indicates two distinct clinical syndromes in sheep, both of which have been ...200111444100
white spot syndrome virus envelope protein vp28 is involved in the systemic infection of shrimp.white spot syndrome virus (wssv) is a large dna virus infecting shrimp and other crustaceans. the virus particles contain at least five major virion proteins, of which three (vp26, vp24, and vp15) are present in the rod-shaped nucleocapsid and two (vp28 and vp19) reside in the envelope. the mode of entry and systemic infection of wssv in the black tiger shrimp, penaeus monodon, and the role of these proteins in these processes are not known. a specific polyclonal antibody was generated against t ...200111437657
dna fragmentation, an indicator of apoptosis, in cultured black tiger shrimp penaeus monodon infected with white spot syndrome virus (wssv).fifty black tiger shrimp penaeus monodon from commercial cultivation ponds in malaysia were examined by tdt-mediated dutp nick-end labeling (tunel) fluorescence assay and agarose gel electrophoresis of dna extracts for evidence of dna fragmentation as an indicator of apoptosis. from these specimens, 30 were grossly normal and 20 showed gross signs of white spot syndrome virus (wssv) infection. of the 30 grossly normal shrimp, 5 specimens were found to be positive for wssv infection by normal his ...200111324818
cytochrome b gene for species identification of the conservation animals.a partial dna sequence of cytochrome b gene was used to identify the remains of endangered animals and species endemic to taiwan. the conservation of animals species included in this study were: the formosan gem-faced civets, leopard cats, tigers, clouded leopards, lion, formosan muntjacs, formosan sika deers, formosan sambars, formosan serows, water buffalo, formosan pangolins and formosan macaques. the control species used included domestic cats, domestic dogs, domestic sheeps, domestic cattle ...200111587860
peroxinectin, a cell adhesive protein associated with the propo system from the black tiger shrimp, penaeus monodon.upon activation of the prophenoloxidase activating system in the shrimp, penaeus monodon, a cell adhesion activity in the haemolymph is generated. a cell adhesion assay showed that a high number of granular cells (60%) adhered to coverslips coated with a shrimp haemocyte lysate supernatant, whereas a very low number of cells adhered to coverslips coated with bovine serum albumin. inhibition of adhesion by an antiserum against crayfish peroxinectin, a cell adhesion protein, revealed that the cell ...200111356216
aortic valvular endocarditis associated with pasteurella haemolytica in a tiger (panthera tigris). 200111700929
penaeus monodon (tiger shrimp) hemocyanin: subunit composition and thermostability.penaeus monodon (class crustacea, order decapoda) is one of the largest shrimps of the penaeidea family from the indo-west pacific region. the dioxygen-transporting protein hemocyanin, isolated from the hemolymph of this invertebrate, is composed of three 75-76 kda structural/functional subunits designated as pm1, pm2 and pm3. the n-terminal sequences of the chains were determined and compared with those of other decapodan hemocyanin subunits. pm2 and pm3 are highly homologous and electrophoreti ...200111421459
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