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cyclic gmp diffusion coefficient in rod photoreceptor outer segments.cyclic gmp (cgmp) is the intracellular messenger that mediates phototransduction in retinal rods. as photoisomerizations of rhodopsin molecules are local events, the longitudinal diffusion of cgmp in the rod outer segment should be a contributing factor to the response of the cell to light. we have employed the truncated rod outer segment preparation from bullfrog (rana catesbeiana) and tiger salamander (ambystoma tigrinum) to measure the cgmp diffusion coefficient. in this preparation, the dist ...19957536055
multiple ion binding sites in ih channels of rod photoreceptors from tiger salamanders.the mechanism of ion permeation in k+/na(+)-permeable ih channels of tiger salamander rod photoreceptors was investigated using the whole-cell voltage-clamp technique. ih channels showed features indicative of pores with multiple ion binding sites: in mixtures of k+ and thallium (t1+), the amplitude of the time-dependent current showed an anomalous mole fraction dependence, and k+ permeation was blocked by other permeant ions (with k0.5 values: t1+, 44 microm; rb+, 220 microm and nh4+, 1100 micr ...19957545280
from paper tigers to consumer-centered quality assurance tools: reforming incident-reporting systems.a practical framework for program administrators seeking to improve the effectiveness of their incident reporting and investigation procedures and practices was provided. too often, these systems, which are designed by outside parties, are viewed by program administrators as instruments of risk exposure rather than viable tools for risk management and quality improvement. program administrators were encouraged to take control of their incident-reporting systems by transforming them into viable c ...19957565146
paper tigers? 20047567531
cone photoreceptors respond to their own glutamate release in the tiger salamander.pulse-like currents resembling miniature postsynaptic currents were recorded in patch-clamped isolated cones from the tiger salamander retina. the events were absent in isolated cones without synaptic terminals. the frequency of events was increased by either raising the osmotic pressure or depolarizing the cell. it was decreased by the application of either glutamate or the glutamate-transport blockers dihydrokainate and d,l-threo-3-hydroxyaspartate. the events required external na+ for which l ...19957568144
nephrotoxicity of notexin in experimental mice.notexin, a well known neurotoxin, derived from tiger snake venom, was shown to have direct nephrotoxic properties in experimental mice. a single subcutaneous dose of 1.38 micrograms/kg body weight of notexin produced renal tubular and glomerular damage within 24 hours. renal damage increased in severity proportional to the dose of notexin injected. at a high dose of notexin, thrombotic "spherules" were found in glomeruli. these thrombotic spherules have not been previously reported to be associa ...19957580101
in vivo diagnosis of hallervorden-spatz disease.the authors present the mri findings of two children with insidious walking difficulties, signs of corticospinal tract involvement, and signs and symptoms of extrapyramidal dysfunction such as rigidity and generalized dystonia, the latter with predominance of oromandibular involvement. in one child, mri revealed prominent hypo-intensity in the globus pallidus and in the substantia nigra on t2-weighted spin echo images, consistent with iron deposition and thus with previous post-mortem findings o ...19957589865
enkephalin immunoreactivity in the paraneurons of the tiger salamander (ambystoma tigrinum) tongue.immunohistochemical tests have demonstrated the presence of leu-5-enkephalin and other bioactive compounds (serotonin and neuron-specific enolase) in the basal cells of lingual taste buds in ambystoma tigrinum; there was also a weak reaction for met-5-enkephalin. similar reactions were obtained from particular cells dispersed within the lingual epithelium, which are provisionally identified as merkel cells.19957603585
verification of water equivalence of femri gels using monte carlo simulation.dosimetric characteristics of the femri gels for various energy photon and electron beams were investigated using the integrated tiger series (its) monte carlo codes. the femri gels have been reported as being tissue-substitutes which can be shaped to any desired contour for three-dimensional (3d) mapping of dose distribution using magnetic resonance (mr) scanning. this study indicated that the central axis depth dose, off-axis depth dose, transverse profile at the depth of 50% of maximum dose, ...19957609731
calcium waves in dissociated retinal glial (müller) cells are evoked by release of calcium from intracellular stores.calcium imaging techniques were used to study intracellular free calcium ion regulation in isolated müller cells in response to changes in extracellular potassium concentration and to caffeine and ryanodine. müller cells were dissociated from the adult tiger salamander (ambystoma tigrinum) retina and studied using the calcium indicator fura-2 and video imaging microscopy techniques. our results demonstrate that elevation of extracellular potassium in the presence of extracellular calcium evokes ...19957615342
dopamine modulates gabac receptors mediating inhibition of calcium entry into and transmitter release from bipolar cell terminals in tiger salamander retina.using optical recording techniques, we directly monitored pre- and postsynaptic calcium dynamics at bipolar cell terminals while inhibiting synaptic release with applied gaba and modulating inhibition with dopamine. to monitor pre-synaptic activity, individual bipolar cells in the retinal slice were filled with either fura-2 or fluo-3 through a patch electrode. calcium entry into bipolar terminals, elicited by depolarization from -60 mv to 0 mv, was reduced to 36% of control in the presence of 2 ...19957623108
comparative anatomy of the cardiac foramen ovale in cats (felidae), dogs (canidae), bears (ursidae) and hyaenas (hyaenidae).the structure of the foramen ovale from 16 species representing 4 carnivore families, the felidae, canidae, ursidae and hyaenidae, was studied using the scanning electron microscope. the felidae were represented by 9 domestic cat fetuses (felis catus), 2 snow leopard neonates (uncia uncia), an ocelot neonate (leopardus pardalis), 2 lion neonates (panthera leo), a panther neonate (panthera pardus) and 3 tigers (neofelis tigris), comprising 2 fetuses and a neonate. the canidae were represented by ...19957649822
similar effects of carbachol and dopamine on neurons in the distal retina of the tiger salamander.though there is considerable evidence that dopamine is an important retinal neuromodulator that mediates many of the changes in the properties of retinal neurons that are normally seen during light adaptation, the mechanism by which dopamine release is controlled remains poorly understood. in this paper, we present evidence which indicates that dopamine release in the retina of the tiger salamander, ambystoma tigrinum, is driven excitatorily by a cholinergic input. we compared the effects of app ...20157654602
ancestry of an isolated subspecies of salamander, ambystoma tigrinum stebbinsi lowe: the evolutionary significance of hybridization.most phylogenetic systematists assume speciation results in dichotomously branching phylogenies. hybridization that gives rise to a new lineage can produce character homoplasty that might obscure a species' true history. we report the results of a restriction-enzyme analysis of mitochondrial dna (mtdna) variation in three tiger salamander subspecies (ambystoma trigrinum mavortium, ambystoma tigrinum nebulosum, and ambystoma trigrinum stebbinsi) and compare the results to studies of morphological ...19957663764
desensitizing glutamate receptors shape excitatory synaptic inputs to tiger salamander retinal ganglion cells.ampa/kainate (ka) receptors mediate a component of ganglion cell excitatory postsynaptic currents (epscs). we investigated whether desensitization at these receptors contribute to the shape of transient epscs in on-off ganglion cells. whole-cell, voltage-clamp recordings were made from ganglion cells in the retinal slice or in isolation. epscs were evoked by either stimulating the slice with light or puffing k+ at the outer plexiform layer (opl). the ampa/ka receptor-mediated component of the ep ...19957666201
quantitative analyses of the coexistence of gamma-aminobutyric acid in substance p-amacrine cells of the larval tiger salamander retina.the present study was performed as part of a systematic examination of gamma-aminobutyric acid's (gaba) coexistence with other classical transmitters and neuropeptides in neuronal populations of the larval tiger salamander retina. substance p immunocytochemistry was combined with either gaba immunocytochemistry or autoradiography of high-affinity gaba uptake to examine for the presence of gaba in substance p-amacrine cells of the larval tiger salamander retina. double-label analyses revealed two ...19937680935
the permeability of the cgmp-activated channel to organic cations in retinal rods of the tiger salamander.1. the permeability of the channel activated by guanosine 3',5'-cyclic monophosphate (cgmp) to many organic monovalent cations was determined by recording macroscopic currents in excised inside-out patches of plasma membrane from isolated retinal rod outer segments of the tiger salamander. 2. current-voltage relations were measured when the nacl of the bathing medium was replaced by salts of organic cations. permeability ratios relative to na+ ions were calculated with the goldman-hodgkin-katz p ...19937683718
divalent cations block the cyclic nucleotide-gated channel of olfactory receptor neurons.1. the effects of external divalent cations on odor-dependent, cyclic amp-activated single-channel currents from olfactory receptor neurons of the tiger salamander (ambystoma tigrinum) were studied in inside-out membrane patches taken from dendritic regions of freshly isolated sensory cells. 2. channels were reversibly activated by 100 microm cyclic amp. in the absence of divalent cations, the channel had a linear current-voltage relation giving a conductance of 45 ps. with increasing concentrat ...19937685377
identification of glutamate receptor subtypes mediating inputs to bipolar cells and ganglion cells in the tiger salamander retina.1. the effects of glutamate receptor agonists and antagonists on bipolar cells and ganglion cells were studied with the use of intracellular and extracellular recording in the superfused, isolated, flat-mounted tiger salamander retina. the goal of the experiments was to correlate glutamate receptor subtypes with their localization at specific synaptic sites in the tiger salamander retina. the drugs tested were the kainate/alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (ampa) receptor a ...19937688801
colocalization of glycine in substance p-amacrine cells of the larval tiger salamander retina.the present study was performed as part of a systematic examination of glycine's coexistence with other classical transmitters and neuropeptides in neuronal populations of the larval tiger salamander retina. substance p immunocytochemistry was combined with either glycine immunocytochemistry or autoradiography of glycine high-affinity uptake to examine whether tiger salamander substance p-amacrine cells express these glycine markers. double-label analyses revealed two populations of substance p- ...20137692942
modulation of transmission gain by protons at the photoreceptor output synapse.synaptic transmission of the light response from photoreceptors to second-order cells of the retina was studied with the whole-cell patch-clamp technique in tiger salamander (ambystoma tigrinum) retinal slices. synaptic strength is modulated by extracellular ph in a striking manner: light-sensitive postsynaptic currents in horizontal and bipolar cells were found to be exponential functions of ph, exhibiting an e-fold increase per 0.23 ph unit over the ph range from 7 to 8. calcium channel curren ...19937694280
a wavelet-based metric for visual texture discrimination with applications in evolutionary ecology.much work on natural and sexual selection is concerned with the conspicuousness of visual patterns (textures) on animal and plant surfaces. previous attempts by evolutionary biologists to quantify apparency of such textures have involved subjective estimates of conspicuousness or statistical analyses based on transect samples. we present a method based on wavelet analysis that avoids subjectivity and that uses more of the information in image textures than transects do. like the human visual sys ...19957696817
analysis of human remains recovered from a shark.trauma caused by marine scavengers and predators, especially sharks, is not well known. this paper describes the effect of shark attack on human remains. they were found in the stomach of a tiger shark caught by fishermen near hollywood beach, florida. the remains belonged to a white male in his late twenties or early thirties with a stature of 175 cm. the damages to the skeleton and the association of these damages with the behaviours of tiger sharks is also analyzed. the trauma affecting long ...19957705731
comparison of the actions of glycine and related amino acids on isolated third order neurons from the tiger salamander retina.whole cell voltage and current clamp recordings were obtained from third order neurons isolated from the salamander retina. using cross desensitization, the structure-function relationship of short chain amino acids on the glycine receptor were examined. l-serine, l-alanine, beta-alanine and taurine all cross desensitized with glycine, but did not show significant cross desensitization with gaba. this indicates that these amino acids act at the glycine receptor. the order of potency was glycine ...19957708201
morphology of ganglion cells in the neotenous tiger salamander retina.the morphology of retinal ganglion cells in the neotenous tiger salamander (ambystoma tigrinum) was analyzed with the aid of morphometric techniques to determine the diversity of cell types and to evaluate the widely held notion that this form of ambystoma has a simple retina, with little variance among its cell morphologies. single-cell staining was achieved through retrograde labeling with horseradish peroxidase injected around the optic nerve sheath followed by a period of several days before ...19957721999
care-planning in long term care.a comprehensive nursing care plan was revised to streamline documentation within a long-term care environment, often a "paper tiger" arena. the care plan guides certified nursing assistants to prevent and identify resident problems while delivering safe care. not only are separate care protocols identified, but the care plan explains the specific nursing interventions to alleviate the residents' concerns or problems.19957731599
amino acid sequence of a new type of toxic phospholipase a2 from the venom of the australian tiger snake (notechis scutatus scutatus).venom from the common tiger snake, notechis scutatus scutatus, contains several toxic acidic proteins which promote hypotension and hemorrhage in mice. one of these toxins, hte, has a phospholipase a2 (pla2) amino acid sequence. it contains 125 amino acids rather than the 119/120 found in other n. s. scutatus pla2s, because it also has the loop of residues (62-66) found in helix d of pancreatic pla2s, the gamma-subunit of taipoxin, and the d-subunit of textilotoxin. high sequence identity is fou ...19957733680
bicuspid evolution of the arterial and venous atrioventricular valves.atrioventricular valves were studied in pisces (shark), amphibians (frog), reptiles (turtle, snake and crocodile) and aves (duck, chicken and quail). dog shark, tiger shark and hammerhead shark have a contractile common atrioventricular orifice with anterior and posterior bridging leaflets. angel shark resembles the frog in having a contractile oval common atrioventricular orifice guarded by two lateral (right and left) and two central bridging (anterior and posterior) leaflets. in turtles and s ...19957742994
homogeneous sample preparation of raw shrimp using dry ice.sample homogeneity is critical to accurate and reproducible analysis of trace residues in foods. a method of uniform sample preparation using dry ice is described for shrimp. other sample preparation techniques for raw shrimp produce nonhomogeneous samples. sample homogeneity was determined through analysis of chloramphenicol added to intact tiger or white shrimp prior to sample preparation. simulated chloramphenicol residue levels were 50, 15, 10, and 5 ppb. no significant differences were note ...20137756906
interaction of anionic and cationic currents leads to a voltage dependence in the odor response of olfactory receptor neurons.1. we recorded odor-induced currents from isolated olfactory receptor neurons of the land phase tiger salamander (ambystoma tigrinum) with the whole cell patch clamp. 2. in a subset of cells the current-voltage relation for the odor-induced current showed a strong rectification with, in some cells, a negative resistance slope between about -45 and -25 mv. in these cells there was little or no odor-induced current at -55 mv, the average resting potential of olfactory neurons. 3. depolarizing the ...19957760118
characterization of auditory afferents in the tiger beetle, cicindela marutha dow.we have identified a nerve carrying auditory afferents and characterized their physiological responses in the tiger beetle, cicindela marutha. 1. the tympana are located at the lateral margins of the first abdominal tergum. the nerve carrying the tympanal afferents is a branch of the dorsal root from the first abdominal ganglion. 2. both male and female auditory afferent responses are sharply tuned to 30 khz with sensitivities of 50-55 db spl. 3. the auditory afferents show little adaptation and ...19957769565
[effectiveness of coils and mats for protection against malaria vectors in cameroon].the evaluation of the effectiveness of coils and mats on mosquitoes, especially malaria vectors was carried out in the southern cameroon. the study was performed in the nsimalen village from november 1993 to august 1994. two brands of coils: timor and moon tiger containing 0.15% w/w of esbiothrin and two brands of mats: ars mats (containing d-allethrin) and moon tiger (with s biothrin and diethyl-toluamide) were tested. the evaluation was based on night catches on human bait inside houses. a cat ...19957780671
evidence for glycine modulation of excitatory synaptic inputs to retinal ganglion cells.the actions of glycine on the nmda receptor-mediated synaptic responses of ganglion cells were studied in the tiger salamander retinal slice. ganglion cell excitatory postsynaptic currents (epscs) were elicited either by exciting bipolar cells with potassium puffs or by light stimulation, and were measured using whole-cell patch-clamp techniques. increasing bath glycine concentrations to 10 microm had little effect on the amplitude of the puff-evoked epscs, indicating either that synaptic glycin ...19957790926
[hair dysplasia in oculo-dento-digital syndrome. apropos of a mother-daughter case].oculo-dento-digital syndrome (sodd) as defined by meyer-schwickerath in 1957 is a rare entity (84 cases) which belongs to ectodermal dysplasias. it consists of: the characteristic features (long face, pinched nose); syndactyly; ocular, dental and bone abnormalities. this entity is usually transmitted on the autosomal dominant mode. we report two cases (a mother and daughter) with polymalformations which we classed as sodd. furthermore, agenesis of lacrimal duct and genitourinary abnormalities we ...19947793757
counter-transport of potassium by the glutamate uptake carrier in glial cells isolated from the tiger salamander retina.1. to investigate the transport of potassium on the glutamate uptake carrier, the glutamate uptake current in isolated retinal müller cells was monitored by whole-cell clamping, while measuring changes of potassium concentration outside the cells ([k+]o) with an ion-sensitive microelectrode. 2. activating glutamate uptake led to an accumulation of potassium outside the cells, consistent with the hypothesis, based on less direct evidence, that the glutamate uptake carrier transports potassium out ...19947837095
glutamate-, gaba-, and gad-immunoreactivities co-localize in bipolar cells of tiger salamander retina.the presence of inhibitory bipolar cells in salamander retina was investigated by a comparative analysis of the distribution of glutamate- and gaba-immunoreactivities (glu-ir; gaba-ir) using a postembedding immunocytochemical method. glu-ir was found in virtually all photoreceptors, bipolar cells and ganglion cells, neuronal elements that transfer information vertically through the retina. glu-ir also was found in numerous amacrine cells in the mid and proximal inner nuclear layer as well as in ...20137841126
a metrical template account of children's weak syllable omissions from multisyllabic words.young children learning english as well as many other languages frequently omit weakly stressed syllables from multisyllabic words. in particular, they are more likely to omit weak syllables from word-initial positions than from word-internal or -final positions. for example, the weak syllable of a weak-strong (ws) word like giraffe is much more likely to be omitted than the weak syllable of a sw word like tiger. three hypotheses for this omission pattern have been offered. in two, children's we ...19947852473
[changes in the lungs in mice caused by migration of toxocara canis larvae].the influence of t. canis larvae migration on the lung tissue of paratenic host (inbred mice, strain c57bl6/j) was evaluated. first macroscopic manifestation was already observed on day 2 in the time of the highest larval recovery. larvae entering the lung tissue caused numerous small extravasations. their migration from the lungs was manifested from day 6 with an increase in the number and extent of extravasations. the lungs assumed tiger spot appearance (fig. 1). the larval recovery was decrea ...19947863576
the expression of vimentin in satellite cells of regenerating skeletal muscle in vivo.the expression of the intermediate filament protein, vimentin, was studied in skeletal muscle during a cycle of degeneration and regeneration. venom from the australian tiger snake, notechis scutatus scutatus, was used to initiate the breakdown of the soleus muscle of young, mature rats in vivo. cryosections and western blots of muscle samples were labelled using antibodies to vimentin, and examined at fixed time points after venom injection. vimentin was absent in control adult muscle fibres, b ...19947896567
aspects of canine distemper virus and measles virus encephalomyelitis.canine distemper (cd) is a frequently fatal, systemic morbillivirus infection in the dog and other carnivores: encephalomyelitis is the common cause of death. susceptibility to canine distemper virus (cdv) is now recognized in a wide range of non-domestic animals, most recently in captive lions, tigers and leopards. furthermore, closely related viruses have produced cd-like diseases in marine mammals. cdv induces an inclusion-body encephalomyelitis in the dog and demyelination is often a conspic ...19947898614
acanthocytosis, retinitis pigmentosa, and pallidal degeneration: a report of three patients, including the second reported case with hypoprebetalipoproteinemia (harp syndrome).we describe an example of a variant of hallervorden-spatz disease, characterized by hypoprebeta-lipoproteinemia, acanthocytosis, retinitis pigmentosa, and pallidal degeneration (harp syndrome), in an 18-year-old woman who presented with longstanding intellectual subnormality, night blindness, and a 2-year history of orobuccolingual dystonia causing dysarthria and dysphagia. investigation showed acanthocytosis and hypoprebetalipoproteinemia, and electroretinograms were typical of tapetoretinal de ...19957898702
parasites of south african freshwater fish. vi. nematode parasites of some fish species in the kruger national park.the nematode parasites of 30 spot-tailed robbers, brycinus imberi, five tiger-fish, hydrocynus vittatus, 77 large-scaled yellowfish. barbus marequensis, two mudsuckers, labeo molybdinus, 114 catfish, clarias gariepinus, 46 silver barbel, schilbe intermedius, 66 squeakers, synodontis zambezensis, three eels, anguilla spp., 83 mozambique bream, oreochromis mossambicus, 81 red-breasted bream, tilapia rendalli swierstrae and 32 large-mouthed bream, serranochromis meridianus, caught in the sabie, cro ...19947898896
replacement of the natural wolbachia symbiont of drosophila simulans with a mosquito counterpart.inherited rickettsial symbionts of the genus wolbachia occur commonly in arthropods and have been implicated in the expression of parthenogenesis, feminization and cytoplasmic incompatibility wolbachia from the asian tiger mosquito, aedes albopictus, to replace the natural infection of drosophila simulans by means of embryonic microinjection techniques. the transferred wolbachia infection behaves like a natural drosophila infection with regard to its inheritance, cytoskeleton interactions and ab ...19947906391
a novel gaba receptor modulates synaptic transmission from bipolar to ganglion and amacrine cells in the tiger salamander retina.we investigated the mechanisms underlying the modulation of transmitter release from bipolar cells. three currents, measured under whole-cell patch clamp in the tiger salamander retinal slice, were used to identify the sites of modulation and to establish their pharmacological profile. (1) a light-elicited inhibitory current was measured in bipolar cells that could be blocked by picrotoxin. this input probably arrives via gabaergic amacrine cells since there is high gaba sensitivity at the bipol ...19947907138
using molecular and ecological data to diagnose endangered populations of the puritan tiger beetle cicindela puritana.populations of the puritan tiger beetle cicindela puritana in the eastern united states were found to be highly threatened at the connecticut river, whereas several large populations on the western shore and newly discovered populations on the eastern shore of the chesapeake bay appeared to be less endangered. we assessed if the disjunct c. puritana subgroups are genetically distinct and therefore should be treated as separate units for conservation purposes. a total of 13 individuals from the c ...19937909261
dopamine enhances a glutamate-gated ionic current in off bipolar cells of the tiger salamander retina.the transmitter glutamate is thought to be used by all vertebrate photoreceptors to drive the second-order neurons of the retina, horizontal and bipolar neurons. dopamine, an endogenous retinal neurotransmitter localized to amacrine and interplexiform cells, has previously been shown to enhance glutamate-gated currents in retinal horizontal cells. in the present study we demonstrate that bipolar cells, like horizontal cells, possess glutamate receptors that are modulated by dopamine. we then ide ...19947931565
parasites of wild felidae in thailand: a coprological survey.ninety-two fecal samples were collected from leopards (panthera pardus, n = 54), tigers (p. tigris, n = 19), and leopard cats (felis bengalensis, n = 3) in huai kha wildlife sanctuary, thailand; four samples that may have come from clouded leopards (neofelis nebulosa) or asian golden cats (felis temminicki) were identified as from small to medium cats. twelve samples were identified as from large cats. samples preserved in 10% formalin were examined for parasite eggs, larvae, cysts, and oocysts ...19947933301
clinical and mri study of the hallervorden-spatz syndrome: long-term follow-up of one case.hallervorden-spatz syndrome is generally considered to be an autosomal recessively hereditary disorder of unknown etiology. some reported cases have been known to be sporadic. we present a boy who suffered from regressive developmental milestones since he was 2 years and 6 months old. he began to manifest tremors of the upper extremities, followed by unsteady gait, choreoathetosis, dystonia, dysarthria, and dysphagia at 4 years old, and subsequently became completely bedridden at 6 years old. ne ...20157942031
canine distemper epizootic in lions, tigers, and leopards in north america.canine distemper virus (cdv) infection occurred in captive leopards (panthera pardus), tigers (panthera tigris), lions (panthera leo), and a jaguar (panthera onca) in 1991 and 1992. an epizootic affected all 4 types of cats at the wildlife waystation, san fernando, california, with 17 mortalities. cdv-infected raccoons were thought to be the source of infection in these cats. two black leopards died at the naibi zoo, coal valley, illinois, and 2 tigers died at the shambala preserve, acton, calif ...19947948195
jamming bat echolocation: the dogbane tiger moth cycnia tenera times its clicks to the terminal attack calls of the big brown bat eptesicus fuscus.certain tiger moths emit high-frequency clicks to an attacking bat, causing it to break off its pursuit. the sounds may either orient the bat by providing it with information that it uses to make an attack decision (aposematism) or they may disorient the bat by interrupting the normal flow of echo information required to complete a successful capture (startle, jamming). at what point during a bat's attack does an arctiid emit its clicks? if the sounds are aposematic, the moth should emit them ea ...19947964403
laboratory study of predation by solenopsis invicta (hymenoptera: formicidae) on eggs of aedes albopictus (diptera: culicidae).the red imported fire ant, solenopsis invicta buren, punctured and fed on the asian tiger mosquito, aedes albopictus (skuse), eggs in the laboratory. the presence and actions of the ants, especially the minima workers, reduced the hatching of egg populations.19947966185
miniature excitatory postsynaptic currents in bipolar cells of the tiger salamander retina.the synapse between photoreceptor and bipolar cell is important for at least three reasons: (1) it is the first synapse in the visual pathway; (2) it is the best-known tonic chemical synapse; and (3) it has perhaps the most complex and highly organized synaptic morphology in the entire brain. yet little is known about how neurotransmitter is released from this synapse. we present in this report evidence which suggests that the release of photoreceptor neurotransmitter, presumably glutamate, is p ...19947975276
the composition and function of the pulmonary surfactant system during metamorphosis in the tiger salamander ambystoma tigrinum.mammalian lungs secrete a mixture of surface-active lipids (surfactant), which greatly reduces the surface tension of the fluid coating the inner lung surface, thereby reducing the risk of collapse upon deflation and increasing compliance upon inflation. during foetal lung maturation, these lipids become enriched in the primary and active ingredient, a disaturated phospholipid. however, disaturated phospholipids exist in their inactive gel-like form at temperatures below 37 degrees c and thus ar ...19947983248
immunostimulation of tiger shrimp (penaeus monodon) hemocytes for generation of microbicidal substances: analysis of reactive oxygen species.nbt staining was used to determine the presence of superoxide anions (o2-) produced by tiger shrimp (penaeus monodon) hemocytes attached to a coverslip. when cells were treated with beta-glucan, blue granules were observed in 41% of studied hemocyte cytoplasm. for zymosan-treated, pma-treated, and control cells, the percentages of hemocytes showing similar blue granules were 31, 9, and 5%, respectively. a comparison of stimulative effects on 15 hemocyte suspensions, each collected from a single ...19948001699
effects of calcium on rod and cone inputs to horizontal cells of the tiger salamander retina.effects of extracellular calcium on signal transmission between photoreceptors and horizontal cells (hcs) are studied in superfused isolated retina of the larval tiger salamander. horizontal cell light response is optimal when extracellular ca2+ is maintained between 1-2 mm. ca2+ levels beyond this range in either direction significantly reduce the hc light response amplitude. when extracellular ca2+ is lowered from 2 mm to 0.5 mm, the rod input to hcs is reduced whereas the cone input is not af ...20138003458
modulation of transduction gain in light adaptation of retinal rods.the effect of light adaptation on the period of photocurrent saturation induced by a bright stimulating flash was examined in rod photoreceptors of the larval-stage tiger salamander (ambystoma tigrinum). using suction electrodes, photocurrent responses to brief flashes were recorded from single, isolated rods in the presence and absence of steady background illumination. background light decreased the saturation period (t) measured at fixed flash intensity (fixed if) and in this respect light-ad ...20088011583
evolution and phylogenetic information content of the its-1 region in the tiger beetle cicindela dorsalis.sequence divergence in the internal transcribed spacer region 1 (its-1) of the ribosomal dna locus was assessed in subspecies of the coastal north american tiger beetle, cicindela dorsalis. the spacer region was amplified using the polymerase chain reaction and cloned for sequencing. of a total of 50 clones obtained from 12 specimens, 42 clones were different in at least one nucleotide position. in a parsimony analysis of these sequences, the main phylogenetic distinction was found to separate s ...19948015434
studies on the formation of perceptually based basic-level categories in young infants.a series of experiments examined the abilities of 3- and 4-month-old infants to form categorical representations to exemplars of natural kinds--cats and horses. these experiments also permitted assessment of the relative exclusivity of these representations--the extent to which they exclude exemplars from contrasting basic-level categories from the same superordinate category. we found that categorical representations could be formed for horses that excluded cats, zebras, and giraffes, and for c ...19948045176
common tiger snake envenomation in dogs and mice--relationship between the amount of venom injected and the onset of clinical signs.common tiger snake (notechis scutatus) venom was injected into mice and dogs at various dose rates calculated on the known lethal dose (ld) for each species. the larger the dose of venom, the earlier was the onset of clinical signs and the more rapid and severe the course of the disease in both species. in dogs injected with 32 ld of venom, there was sudden collapse and death in about one hour from the time of injection without recovery from premonitory depression and before mydriasis occurred. ...19948067944
some toxicity thresholds for the clinical effects of common tiger snake (notechis scutatus) envenomation in the dog.common tiger snake (notechis scutatus) venom was administered experimentally to dogs at doses from 0.25 lethal dose (ld) to 20 ld. haemolysis and increased creatine kinase values occurred rapidly after injection of sublethal (subparalytic) doses, but the clotting time of blood was extended and blood became incoagulable only when dogs were dosed with 10 ld or more of venom. haemolysis, although of a low threshold of toxicity, was not severe and should not greatly affect the lethality of the venom ...19948067945
myotoxicity and nephrotoxicity of common tiger snake (notechis scutatus) venom in the dog.the myotoxicity and neurotoxicity of common tiger snake (notechis scutatus) venom are major factors in the pathogenesis of envenomation in the dog. histological examination of the tissues of experimentally envenomed dogs has demonstrated the importance of muscle damage in affecting the clinical syndrome of tiger snake envenomation. within one hour of injection of the venom into dogs, there was selective involvement of some muscles. cardiac and smooth muscles were not significantly affected. the ...19948067946
characterization of the glutamate transporter in retinal cones of the tiger salamander.l-glutamate elicits an inwardly rectifying current at hyperpolarized potentials in isolated retinal cones of the tiger salamander, as measured under whole-cell patch clamp. evidence presented in this article supports the notion that cones possess a high-affinity glutamate transporter. this glutamate-elicited current shows no desensitization over a period of several minutes, and has an affinity (km) of 10 microm. the inward current is mimicked by the amino acids l-aspartate, d-aspartate, l-cystea ...19938093715
synaptic organization of dopaminergic amacrine cells in the larval tiger salamander retina.the ultrastructural features and synaptic interactions of tyrosine hydroxylase-like-immuno-reactive amacrine cells in the larval tiger salamander retina were examined using routine immunoelectron microscopy. the somas of tyrosine hydroxylase-like-immunoreactive amacrine cells were immunostained evenly throughout their cytoplasm. their nuclei were generally unstained and possessed indented nuclear membranes. the processes of tyrosine hydroxylase-like-immunoreactive amacrine cells were homogeneous ...19938098517
double-label analyses of the coexistence of somatostatin with gaba and glycine in amacrine cells of the larval tiger salamander retina.to investigate the possible gabaergic nature of somatostatin-immunoreactive neurons of the larval tiger salamander retina, somatostatin immunocytochemistry was combined with either gamma-aminobutyric acid (gaba) immunocytochemistry or autoradiography of gaba high-affinity uptake. a total of 1,062 somatostatin cells were visualized in these studies. double-label immunocytochemistry revealed that 96.3% of somatostatin-immunoreactive cells expressed gaba immunoreactivity. double-label studies combi ...19938104080
taming the tiger. the economics of health care reform: an h&hn study.though legislators, regulators and policy-makers have tried to control it for years, health care spending continues to spiral out of control. the question is: will federal health care reform tame it? using a specially developed survey of health care providers, contributing editor alden solovy has developed an analysis of various economic scenarios coming out of the reform process.19948111446
a novel gaba receptor on bipolar cell terminals in the tiger salamander retina.we studied the pharmacology of the gaba receptors on bipolar cell terminals in the retinal slice preparation. whole-cell patch-clamp recordings were made from the somas of bipolar cells and gaba was puffed near their terminals, after synaptic transmission was blocked. gaba puffs evoked a large chloride current that was reduced by picrotoxin, but in many cells this current was insensitive to blockade by the competitive gabaa receptor antagonists bicuculline and sr95531. pentobarbital, an enhancer ...19948120620
[bacterial infections in felines in zoological gardens].the significance of selected bacterial infections of felines in zoological gardens is discussed. anthrax and malleus are in germany of historical interest only, tuberculosis went down very strongly. isolated cases of pseudotuberculosis must be expected in future. infections with bacteria of the genera chlamydia, pasteurella and of the family enterobacteriaceae are also considered. the significance of clostridium perfringens in the so called tiger disease is discussed.19948129702
mechanism of ba2+ block of m-like k channels of rod photoreceptors of tiger salamanders.ikx is a voltage-dependent k+ current in the inner segment of rod photoreceptors that shows many similarities to m-current. the depression of ikx by external ba2+ was studied with whole-cell voltage clamp. ba2+ reduced the conductance and voltage sensitivity of ikx tail currents and shifted the voltage range over which they appeared to more positive potentials. these effects showed different sensitivities to ba2+: conductance was the least sensitive (k0.5 = 7.6 mm), voltage dependence intermedia ...19948169597
synaptic transmission from rods to rod-dominated bipolar cells in the tiger salamander retina.synaptic transmission between photoreceptors and bipolar cells was studied in dark-adapted tiger salamander retinas. based on the relative light sensitivity, bipolar cells, either depolarizing (dbc) or hyperpolarizing (hbc), fell into two groups: one receives inputs primarily from rods (rod-dominated bipolar cells, dbcr and hbcr) and the other receives inputs primarily from cones (cone-dominated bipolar cells, dbcc and hbcc). the input-output relations of the rod-dbcr and rod-hbcr synapses were ...19938186975
saving the tiger in the wild. 19948196759
neutralization of tiger snake (notechis scutatus) venom by serum from other australian elapids.sera from four australian elapids and one boidid (python) were tested for their ability to protect neonatal mice against the toxic action of tiger snake (notechis scutatus) venom. of the five serum samples tested, only serum from pseudechis australis and pseudechis porphyriacus were capable of neutralizing the tiger snake venom. in addition, neutralization was shown to be highly variable within serum taken from individual snakes of the same species (p. porphyriacus). previously, only viperid and ...19938212036
chronic cryptosporidiosis in australian elapid snakes: control of an outbreak in a captive colony.an outbreak of chronic cryptosporidiosis resulting in hypertrophic gastritis occurred in a captive colony of australian elapid snakes. two species of the genus notechis were involved: notechis ater (black tiger snake) and notechis scutatus (eastern or mainland tiger snake). the infection was eventually fatal in all 9 affected snakes. typical histopathological findings of the stomach included mucosal thickening with cystic dilatation of gastric glands, moderate oedema and fibrosis of the lamina p ...19938216094
measurements of faecal oestradiol and progesterone in non-pregnant and pregnant domestic and exotic cats.faecal samples collected for variable periods from 12 animals and five species of cats were assayed for progesterone and oestradiol content by application of standard radioimmunoassays to aliquots (50 microliters) of methanol extracts (4 ml) of a mixture of 0.5 g sample, 0.5 ml water and 1 g aluminium oxide, following partitioning of the total extract with petroleum ether (3 ml), further dilution of assay aliquots and drying. recoveries averaged 100 and 72% for oestradiol and progesterone, respe ...19938229917
gonadotropin-releasing hormone agonist binding in tiger salamander nasal cavity.binding of the iodinated gonadotropin-releasing hormone (gnrh) agonist, buserelin, was examined in the nasal cavities of tiger salamanders using in vitro autoradiography. binding of [125i]buserelin was seen within the chemosensory epithelium of the main nasal cavity and jacobson's (vomeronasal) organ. highest levels of binding were observed over the chemosensory neuron dendrites. given the apparent lack of gnrh-immunoreactive fibers within the chemosensory epithelium as we have observed in a pre ...19938247354
the relation between stimulus and response in olfactory receptor cells of the tiger salamander.1. olfactory receptor cells were isolated from the adult tiger salamander ambystoma tigrinum and the current in response to odorant stimuli was measured with the whole-cell voltage-clamp technique while odorants at known concentrations were rapidly applied for controlled exposure times. 2. three odorants, cineole, isoamyl acetate and acetophenone, were first applied at 5 x 10(-4) m. out of forty-nine cells tested, 53% responded to one odorant only, 22% to two odorants and 25% to all three odoran ...19938254501
structural description of agrammatic comprehension.the trace-deletion hypothesis (grodzinsky, 1990) holds that the comprehension deficit apparent in most agrammatic aphasics results from the absence of traces at the level of s-structure. this paper reports a test of this hypothesis in a case study of an agrammatic aphasic. two experiments--one using a sentence-picture matching task, one using the truth-value judgment task-examined the comprehension of the matrix clause in center-embedded relatives such as, the tiger that chased the lion is big. ...19938269331
interfacing u.s. census map files with statistical graphics software: application and use in epidemiology.in 1990, the united states bureau of the census released detailed geographic map files known as tiger/line (topologically integrated geographic encoding and referencing). the tiger files, accessible through purchase or federal repository libraries, contain 24 billion characters of data describing various geographic features including coastlines, hydrography, transportation networks, political boundaries, etc. for the entire united states. many of these physical features are of potential interest ...19938272673
a comparative study on the biological properties of venoms from juvenile and adult common tiger snake (notechis scutatus) venoms.1. the biological properties of venoms from juvenile and adult common tiger snakes (notechis scutatus) were compared. 2. the lethality, procoagulant activity and enzymatic activities of the juvenile venom were not substantially different from those of the adult venom. 3. electrophoretic studies, however, indicated some minor differences in the protein composition of the juvenile and adult venoms.19938281760
sodium channels aggregate at former synaptic sites in innervated and denervated regenerating muscles.the role of innervation in the establishment and regulation of the synaptic density of voltage-activated na channels (nachs) was investigated at regenerating neuromuscular junctions. rat muscles were induced to degenerate after injection of the australian tiger snake toxin, notexin. the loose-patch voltage clamp technique was used to measure the density and distribution of nachs on muscle fibers regenerating with or without innervation. in either case, new myofibers formed within the original ba ...19948294497
distribution and morphology of motoneurons innervating different muscle fiber types in an amphibian muscle complex.the distribution and morphology of motoneurons innervating specific types of muscle fibers in the levator scapulae superior (lss) muscle complex of the bullfrog (rana catesbeiana) and tiger salamander (ambystoma tigrinum) were studied by retrograde labelling with cholera toxin-conjugated horseradish peroxidase (ct-hrp). the lss muscle complex in both of these amphibians has a segregated pattern of muscle-fiber types (tonic; fast oxidative-glycolytic twitch [fog]; fast glycolytic twitch [fg]) alo ...19938315651
aedes albopictus in europe: a new challenge for dermatologists.a patient is reported who was bitten by aedes albopictus, commonly known as tiger mosquito. the biology of the insect, originally living in the forests of southeastern asia, and its role in transmitting exotic infectious agents is discussed. up to this day, only albania and northern italy have been colonized, but the biologic characteristics of the insect make its spreading all over europe highly probable, unless serious attempts to eradicate it are quickly undertaken. dermatologists should thin ...19938324281
notechis scutatus venom increases the yield of proliferating muscle cells from biopsies of normal and dystrophic canine muscle--a possible source for myoblast transfer studies.the injection of 1 micrograms notechis scutatus (australian tiger snake) venom (notexin) induces localized necrosis in the muscles of normal and dystrophic dogs. biopsies taken from the muscles on the second day of postnecrotic regeneration provide about 8-16 x 10(6) cells capable of proliferation per g tissue, about 100 fold more than the untreated adult dog muscles. muscle specific markers, such as the capacity of the cells to fuse, surface labelling with n-cam antibodies (leu-19 and 5.1.h11), ...19938329886
effects of gaba on horizontal cells in the tiger salamander retina.application of gamma-aminobutyric acid (gaba) slows down the horizontal cell response time course (hcrrt) and induces membrane depolarization in horizontal cells (hcs) after synaptic inputs are blocked by co2+. we present evidence that suggests both effects are probably mediated by gabaa receptors which open chloride channels in the hc membrane. in any given concentration of gaba, ranged from 0 to 100 microm, the hc membrane potential (vhc) in saturating light and in the presence of 100 microm c ...19938333157
taming the paper tiger. 19938334400
relative contribution of rod and cone inputs to bipolar cells and ganglion cells in the tiger salamander retina.1. the relative contribution of rod and cone inputs to bipolar and ganglion cells were studied by comparing the response-irradiance relations, spectral sensitivities, and response waveforms of these neurons recorded from the isolated, flat-mounted tiger salamander retina under dark-adapted conditions. 2. bipolar cells could be differentiated both on the basis of the polarity of the light response and on their relative rod/cone input. thus some depolarizing bipolar cells appeared more strongly in ...19938350133
effects of prolactin, growth hormone, and triiodothyronine on prolactin receptors in larval and adult tiger salamanders (ambystoma tigrinum).the effects of porcine growth hormone (pgh) or ovine prolactin (oprl) alone and in combination with triiodothyronine (t3) on renal prl receptors were determined in both pre- and post-metamorphic tiger salamanders (ambystoma tigrinum). the protein hormones were given at a dose of 1.0 micrograms/gm body weight/day and the t3 was given at 10.0 ng/gm body weight/day. the duration of treatment was 7 days. effects on growth, and plasma thyroid hormone levels were also determined. ovine prl increased g ...19938354992
peripheral projections of nervus terminalis lhrh-containing neurons in the tiger salamander, ambystoma tigrinum.the peripheral projections of the nervus terminalis (nt) have been difficult to examine due to the weak immunoreactivity of the processes to various antibodies. we performed two experimental manipulations in the tiger salamander in an attempt to increase the luteinizing hormone-releasing hormone-immunoreactive (lhrh-ir) labelling in the peripheral processes of the nt:1) the nt was sectioned centrally, or 2) a 100 mg melatonin pellet was embedded subcutaneously for 3 days prior to sacrifice. foll ...19938364959
aedes albopictus: the tyre-travelling tiger. 19938370933
contribution of the ciliary cyclic nucleotide-gated conductance to olfactory transduction in the salamander.1. flash photolysis of caged cyclic nucleotides was used to examine the contribution of the ciliary cyclic nucleotide-gated conductance to olfactory transduction in the tiger salamander. brief illumination of solitary olfactory receptor cells loaded with 100 microm caged cyclic amp caused a large inward current (peak amplitude 355 +/- 200 pa; mean +/- s.d. for eleven cells) under whole-cell voltage clamp at -50 mv. 2. the photolysis response was initiated after a latency of 4-12 ms, whereas an o ...19938392566
hallervorden-spatz disease: mr and pathologic findings.to compare the mr findings of eight cases with clinical diagnosis of hallervorden-spatz disease (hsd) with the pathologic findings of two other cases of hsd.19938427079
mammalian spinal biomechanics. i. static and dynamic mechanical properties of intact intervertebral joints.four-point bending was used to apply pure extension and flexion moments to the ligamentous lumbosacral spine and pelvic girdle of monkey (macaca fascicularis), rabbit (domestic and wild, oryctolagus cuniculus), badger (meles meles), wallaby (wallabia rufogrisea frutica), sheep (ovis aries), seal (phoca vitulina) and tiger (panthera tigris). the absolute ranges of angular change in lumbar-lumbar joints (from x-radiographs) were considerable and similar in monkey and wallaby (greater in flexion) a ...19938440968
mammalian spinal biomechanics. ii. intervertebral lesion experiments and mechanisms of bending resistance.three-point cyclic bending was applied to intervertebral joint complexes (three vertebrae with two intervertebral discs) of monkey (macaca fascicularis), wallaby (wallabia rufogrisea frutica), tiger (panthera tigris), jaguar (panthera onca) and seal (halichoerus grypus). force-displacement loops were recorded for intact specimens in both extension and flexion. reductions in peak forces at given displacements were measured, following lesions of ventral ligaments, superspinous ligaments, interspin ...19938440969
a new case of isolated trichothiodystrophy.we describe a new case of isolated trichothiodystrophy. this entity is characterized by sparse and brittle hair, low sulfur hair content, tiger tail pattern of the hair under polarizing microscopy, clean transverse fractures through the hair shaft and absent or defective hair cuticles. to our knowledge there are only two reported cases of isolated trichothiodystrophy without associated additional ectodermal or neuroectodermal dysplasias. polarized microscopy of cut hair showed transverse fractur ...19938453147
[chronic gastritis caused by ollulanus tricuspis (leuckart, 1865) in a tiger].a chronic gastritis caused by an infestation with o. tricuspis is described. the differential-diagnostic importance of this parasitosis in chronic maldigestive diseases is emphasized. conventional coproscopic diagnostic procedures fail as the life cycle of the parasite is confined to the gastrum of the host.19938457185
kinship affects morphogenesis in cannibalistic salamanders.inclusive fitness theory predicts that organisms can often increase their fitness by helping relatives. indeed, many animals modify their behaviour towards kin in a fashion consistent with theory. morphogenesis may also be sensitive to kinship environment, especially in species that facultatively produce distinct morphs that differ in their ability to harm relatives, such as those that produce alternative cannibalistic and non-cannibalistic phenotypes. we tested this hypothesis by examining whet ...19938479520
fine structure of the retinal epithelium of the tiger salamander (ambystoma tigrinum).the retinal pigment epithelium (rpe) of the tiger salamander (ambystoma tigrinum) consists of a single layer of large squamous-shaped cells. these rpe cells are but minimally infolded basally (sclerally) but show numerous apical (vitreal) processes that interdigitate with the photoreceptor cells. a series of apically-located tight junctions joins the rpe cells to form part of the blood-ocular barrier. internally the rpe nucleus is large, vesicular and flattened. smooth endoplasmic reticulum pred ...19938490252
fine structure of the retinal photoreceptors of the tiger salamander (ambystoma tigrinum).the retinal photoreceptors of the tiger salamander (ambystoma tigrinum) have been studied by light and electron microscopy in both light- and dark-adaptation. rods and cones are present in this duplex retina in a ratio of about 20:1. as in other urodele species these photoreceptors are very large cells. the rod outer segment is composed of bi-membranous discs of uniform diameter displaying several very deep incisures. the rod inner segment displays an ellipsoid of mitochondria and a myoid region ...19938490253
proteins isolated from the venom of the common tiger snake (notechis scutatus scutatus) promote hypotension and hemorrhage.notechis scutatus scutatus venom contains several toxic acidic proteins called hta-i which promote hypotension and hemorrhage in mice. they have apparent mol. wts in the 18,000-21,000 range, i.v. ld50 values between 0.5 and 1.5 micrograms/g, and no detectable phospholipase, arginine esterase, proteolytic or hemolytic activities. a polyclonal antibody raised against htg binds to other purified proteins, suggesting that they are isoforms of the same protein. many other elapid crude venoms contain ...19938503133
[contribution of nuclear magnetic imaging in the diagnosis of hallervorden-spatz syndrome].the manifestations of the hallervorden-spatz disease (hsd) usually appear during childhood or in adults. they are not specific and magnetic resonance imaging (mri) has improved its diagnosis.19938507137
n-acetyltransferase, hydroxyindole-o-methyltransferase and melatonin in the optic lobes of the giant tiger shrimp penaeus monodon.the activities of the enzymes n-acetyltransferase (nat) and hydroxyindole-o-methyltransferase (hiomt) and the hormone melatonin were studied in the optic lobe of the subadult giant tiger shrimp penaeus monodon. compared with the level in other species, a relatively high level of nat activity that was temperature- and ph-dependent were observed. the nat enzyme had a relatively high maximum velocity (vmax, 100 pmol/hr/micrograms protein) and low michaelis constant (km, 22 microm), when tryptamine ...19958531052
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