TitleAbstractYear(sorted ascending)
shedding of toxoplasma gondii oocysts by felidae in zoos in the czech 1995 and 1996, the shedding of toxoplasma gondii oocysts was monitored in the faeces of felidae in six zoos in the czech republic. in all, 2287 samples of faeces from 19 species of felidae were examined. in ostrava zoo, four episodes of shedding of toxoplasma-like oocysts were identified, using a flotation examination, in a pair of wild cats (felis silvestris), six episodes in a wild cat held separately, and three episodes in a pair of amur leopard cats (f. euptilurus). after the passage of s ...19989493305
[use of rapd-pcr-analysis of cellular dna for the evaluation of genetic polymorphism and subspecies diagnostics of the far eastern leopard panthera pardus orientalis].genetic polymorphism in the far-eastern leopard subspecies panthera pardus orientalis was examined by rapd-pcr analysis of total cellular dna. primers diagnostic for the leopard subspecies were detected. these primers can be used for differentiating wild-living animals from those kept in captivity and distinguishing different breeding lines. genetic diversity was shown to be lower in animals from the zoo (d = 0.058) and higher in a natural population (d = 0.137). two lines of the persian subspec ...199910495952
irradiation of diets fed to captive exotic felids: microbial destruction, consumption, and fecal consistency.two frozen, raw horse meat-based diets fed to captive exotic felids at brookfield zoo were irradiated to determine the extent of microbial destruction and whether radiation treatment would affect consumption and/or fecal consistency in exotic cats. fifteen cats, two african lions (panthera leo), two amur tigers (panthera tigris altaica), one amur leopard (panthera pardus orientalis), two clouded leopards (neofelis nebulosa), two caracals (felis caracal), one bobcat (felis rufus), and five fishin ...200112785680
clostridium perfringens enterotoxicosis in two amur leopards (panthera pardus orientalis).two 6-yr-old male sibling amur leopards (panthera pardus orientalis) housed together at the pittsburgh zoo presented for acute onset of diarrhea with no changes in appetite or behavior. heat-fixed modified wright-stained and gram-stained fecal smears revealed a mixed bacterial population with a large number of gram-positive clostridium perfringens-like spores (>20 per high-power oil immersion field). in addition, c. perfringens enterotoxin was isolated from one leopard at 1:256, confirming the p ...200112790411
applying molecular genetic tools to the conservation and action plan for the critically endangered far eastern leopard (panthera pardus orientalis).a role for molecular genetic approaches in conservation of endangered taxa is now commonly recognized. because conservation genetic analyses provide essential insights on taxonomic status, recent evolutionary history and current health of endangered taxa, they are considered in nearly all conservation programs. genetic analyses of the critically endangered far eastern, or amur leopard, panthera pardus orientalis, have been done recently to address all of these questions and develop strategies fo ...200314558456
a non-surgical uterine lavage technique in large cats intended for treatment of uterine infection-induced infertility.this paper presents the successful use of a non-surgical, transcervical uterine lavage technique for the treatment of uterine infection-induced infertility in three female large cats. we developed a non-surgical uterine lavage technique, which allowed repeated flushing of the uterine lumen and installation of therapeutic antibiotics. the entire procedure was performed under general anaesthesia (duration of anesthesia ranged from 40 to 70 min). it was successfully applied in a sumatran tiger (pan ...200616530816
disseminated coccidioidomycosis in a captive indochinese tiger (panthera tigris corbetti) with chronic renal disease.a 19-yr-old, 78.2-kg captive female indochinese tiger (panthera tigris corbetti) from the el paso zoo (el paso, texas, usa) with chronic renal disease was euthanized after a 10-day course of anorexia, depression, progressive rear limb weakness, muscle fasciculations, and head tremors. postmortem findings included pericardial effusion, generalized lymphadenopathy, glomerulosclerosis, glomerular atrophy with membranous glomerulonephropathy, and pancreatic adenocarcinoma. pyogranulomatous pneumonia ...200617315442
seroepidemiology of toxoplasma gondii in zoo animals in selected zoos in the midwestern united states.toxoplasma gondii infections in zoo animals are of interest because many captive animals die of clinical toxoplasmosis and because of the potential risk of exposure of children and elderly to t. gondii oocysts excreted by cats in the zoos. seroprevalence of t. gondii antibodies in wild zoo felids, highly susceptible zoo species, and feral cats from 8 zoos of the midwestern united states was determined by using the modified agglutination test (mat). a titer of 1:25 was considered indicative of t. ...200818605803
conservation genetics of the far eastern leopard (panthera pardus orientalis).the far eastern or amur leopard (panthera pardus orientalis) survives today as a tiny relict population of 25-40 individuals in the russian far east. the population descends from a 19th-century northeastern asian subspecies whose range extended over southeastern russia, the korean peninsula, and northeastern china. a molecular genetic survey of nuclear microsatellite and mitochondrial dna (mtdna) sequence variation validates subspecies distinctiveness but also reveals a markedly reduced level of ...200812547918
The complete mitochondrial genome sequence of the Amur leopard cat, Prionailurus bengalensis euptilurus.The total length of the Prionailurus bengalensis euptilurus genome is 16,990 bp, with a total base composition of 33% A, 27.4% T, 26% C, and 13.5% G. The base compositions present clearly the A-T skew, which is most obvious in the tRNA genes (64%).201122040073
canine distemper virus: an emerging disease in wild endangered amur tigers (panthera tigris altaica).fewer than 500 amur tigers (panthera tigris altaica) remain in the wild. due to low numbers and their solitary and reclusive nature, tiger sightings across their range in the russian far east and china are rare; sightings of sick tigers are rarer still. serious neurologic disease observed in several wild tigers since 2001 suggested disease emergence in this endangered species. to investigate this possibility, histology, immunohistochemistry (ihc), in situ hybridization (ish), and reverse transcr ...201323943758
a review of the proposed reintroduction program for the far eastern leopard (panthera pardus orientalis) and the role of conservation organizations, veterinarians, and zoos.the amur leopard is at the point of extinction. at present there are fewer than 35 in the wild. their natural habitat ranges from china to the north korean peninsula to primorsky krai in russia. a reintroduction plan has been proposed to increase the population in the wild; however, this proposed plan still has many questions to be answered as to how effective it will be. the main objective is to reintroduce animals from a select group within the far eastern leopard programme or the species surv ...201324331556
patent ductus arteriosus in an adult amur leopard (panthera pardus orientalis).a clinically healthy 16-yr-old female leopard (panthera pardus orientalis) was diagnosed with a patent ductus arteriosus on echocardiography and later confirmed on necropsy a murmur was heard on auscultation during a routine examination, and the congenital defect was an incidental finding. the animal had been asymptomatic its entire life. this deformity is rarely observed in nondomestic felids and may be asymptomatic, as has been described in domestic cats.201323505729
identifying ecological corridors for amur tigers (panthera tigris altaica) and amur leopards (panthera pardus orientalis).the rapid explosion of human populations and the associated development of human-dominated landscapes have drastically reduced and fragmented habitat for tigers (panthera tigris) and leopards (panthera pardus) across asia, resulting in multiple small populations. however, amur tiger (panthera tigris altaica) habitat in russia has remained largely interconnected, except for a break between tigers in southwest primorye and the southern sikhote-alin mountains. this habitat patch in southwest primor ...201526096589
[new evidence for the spread of thelazia callipaeda in the far east].thelazia callipaeda nematodes parasitize in the eyes of domestic and wild carnivorous mammals (more often in canidae). numerous cases of human infestation are also known. the nematode spreads in south and east asia although in the last decade this has been reported from dogs, cats and wolves in south and central europe as well. in the russian federation, t. callipaeda was earlier observed in dogs, cats, foxes and raccoon dogs in some regions of the russian far east. two cases of human infestatio ...201525850318
prevalence of valvular regurgitations in clinically healthy captive leopards and cheetahs: a prospective study from the wildlife cardiology (wlc) group (2008-2013).the purpose of this prospective study was to evaluate transthoracic echocardiograms from clinically healthy large felids for the presence of valvular regurgitations (vr). physiologic vr commonly occur in normal dogs and cats, but the percentage of large felids with vr has not been previously reported. during a 5-yr study period (2008-2013), 28 healthy animals were evaluated under general anesthesia: 16 cheetahs (acinonyx jubatus soemmeringuii) with a mean age of 1.5±0.8 yr (range 0.7-3.5 yr), 5 ...201526352956
new hope for the survival of the amur leopard in china.natural range loss limits the population growth of asian big cats and may determine their survival. over the past decade, we collected occurrence data of the critically endangered amur leopard worldwide and developed a distribution model of the leopard's historical range in northeastern china over the past decade. we were interested to explore how much current range area exists, learn what factors limit their spatial distribution, determine the population size and estimate the extent of potentia ...201526638877
comparison of carnivore, omnivore, and herbivore mammalian genomes with a new leopard assembly.there are three main dietary groups in mammals: carnivores, omnivores, and herbivores. currently, there is limited comparative genomics insight into the evolution of dietary specializations in mammals. due to recent advances in sequencing technologies, we were able to perform in-depth whole genome analyses of representatives of these three dietary groups.201627802837
conservation of spermatogonial stem cell marker expression in undifferentiated felid spermatogonia.spermatogonial stem cells (sscs) are distinct in their ability to self-renew, transmit genetic information, and persist throughout the life of an individual. these characteristics make sscs a useful tool for addressing diverse challenges such as efficient transgenic production in nonrodent, biomedical animal models, or preservation of the male genome for species in which survival of frozen-thawed sperm is low. a requisite first step to access this technology in felids is the establishment of mol ...201627129396
reconstructing the historical distribution of the amur leopard (panthera pardus orientalis) in northeast china based on historical records.the range of the amur leopard (panthera pardus orientalis) has decreased dramatically over the last 100 years. this species is still under extreme risk of extinction and conservation efforts are rigorous. understanding the long-term dynamics of the population decline would be helpful to offer insight into the mechanism behind the decline and endangerment and improve conservation perspectives and strategies. historical data collection has been the challenge for reconstructing the historical distr ...201627408548
a trial of semen collection by transrectal electroejaculation method from amur leopard cat (prionailurus bengalensis euptilurus).we collected semen from a male amur leopard cat using the transrectal electroejaculation method and investigated the semen qualities for about four years. in addition, the influence of the season on the spermatogenic function of the amur leopard cat was investigated with regard to the semen qualities, testicular volume and serum testosterone level. as a result, we could collect semen with good sperm qualities that would be useable for artificial insemination. some seasonality was noted in the te ...201626935841
noninvasive genetics provides insights into the population size and genetic diversity of an amur tiger population in china.understanding population size and genetic diversity is critical for effective conservation of endangered species. the amur tiger (panthera tigris altaica) is the largest felid and a flagship species for wildlife conservation. due to habitat loss and human activities, available habitat and population size are continuously shrinking. however, little is known about the true population size and genetic diversity of wild tiger populations in china. in this study, we collected 55 fecal samples and 1 h ...201626663614
[individual identification of amur leopards (panthera pardus orientalis) using molecular-genetic methods and estimation of the population].for the first time, the genetic structure of a population of amur leopards (panthera pardus orientalis) in southwest primorie was analyzed in detail. in 2010-2012, 23 individuals were identified individually. it was shown that the studied microsatellite markers are suitable for individual identification of leopards, monitoring the population numbers, and creating a unified database of genetic profiles of this species to solve research and nature-preserving tasks.201623789419
intrauterine insemination with fresh semen in amur leopard cat (pionailurus bengalensis eutilura) during non-breeding season.equine and human chorionic gonadotropins were administered to two female amur leopard cats to induce estrus and ovulation during non-breeding season. fresh semen collected from male cats was surgically inseminated into the uterine horn of the females. in one animal, two fetal sacs without heartbeats were observed on abdominal ultrasonography 31 days after insemination, which indicated that embryo death had occurred. in the other animal, fetal heartbeats were detected in two fetal sacs 29 days af ...201727733725
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