| partial purification and properties of ovine liver echinococcus granulosus protoscolices phospholgucose isomerase. | echinococcus granulosus protoscolex is the actual larval stage of the cestode causing echinococcosis both in man and animals. in the present report, certain properties of phosphoglucose isomerase from the ovine liver e. granulosus protoscolices have been studied and compared with those of the hydatid cyst fluid and the healthy ovine liver enzymes. the protoscolices enzyme prepared in a manner similar to the hydatid cyst fluid and the ovine liver enzymes exhibited the following properties: (1) ph ... | 1976 | 4246 |
| biochemical differences between the mb and mm isoenzymes of creatine kinase. | the biochemical properties of partially purified preparations of human myocardial mb and mm iosenzymes of creatine kinase have been compared with one another. differences in substrate affinity, behaviour with inhibitors, and heat denaturation have been demonstrated. it is doubtful if the differences we have shown are sufficiently great to form the basis of a practical routine procedure for measuring serum levels of the mb isoenzyme. | 1976 | 4247 |
| alkylation of estradiol 17beta-dehydrogenase from human placenta with 3-chloroacetylpyridine--adenine dinucleotide. | 3-chloroacetylpyridine--adenine dinucleotide, which is active as a hydride acceptor (km = 0.6 mm), inactivates and alkylates estradiol 17beta-dehydrogenase. the kinetics of inactivation by 3-chloroacetylpyridine--adenine dinucleotide and the absence of inactivation by 3-chloroacetylpyridine ribose phosphate show that the alkylation follows the formation of a binary complex (kd = 4.5 x 10(-4) m). studies of the labelling by 3-chloro2-14cacetylpyridine--adenine dinucleotide and the rate of alkylat ... | 1976 | 4323 |
| the effect of fructose infusions on the oxygen transport system of human blood. | in 9 healthy subjects the erythrocyte 2,3-diphosphoglycerate (2,3-dpg) concentration, which modifies the oxygen affinity of haemoglobin, decreased by more than 25 per cent within 60 minutes of the beginning of a fructose infusion (0.5 g.kg body weight-1.h-1). in contrast erythrocyte adenosine triphosphate (atp) concentration was unchanged. in spite of the diminished 2,3-dpg concentrations the oxygen affinity of whole blood actually measured remained unaltered. however, at the same time both the ... | 1976 | 4331 |
| intensive design in medical research. | | 1975 | 4824 |
| anion binding to proteins. nmr quadrupole relaxation study of chloride binding to various human hemoglobins. | | 1976 | 4339 |
| further studies on the fertility promoting factor from human seminal plasma. | a small molecular weight substance from human seminal plasma has been further purified by chromatography. the fertility promoting action of this factor on epididymal sperm has been confirmed in mouse in vitro and in vivo. experimental evidence indicates that the factor acts on the sperm and not on the eggs. its possible mode of action is by improving the motility and survival of the epididymal sperm. | 1975 | 4391 |
| studies of human kidney gamma-glutamyl transpeptidase. purification and structural, kinetic and immunological properties. | gamma-glutamyl transpeptidase, present in various mammalian tissues, transfers the gamma-glutamyl moiety of glutathione to a variety of acceptor amino acids and peptides. this enzyme has been purified from human kidney cortex about 740-fold to a specific activity of 200 units/mg of protein. the purification steps involved incubation of the homogenate at 37 degrees followed by centrifugation and extraction of the sediment with 0.1 m tris-hcl buffer, ph 8.0, containing 1% sodium deoxycholate; batc ... | 1976 | 4442 |
| binding and degradation of insulin by human peripheral granulocytes. demonstration of specific receptors with high affinity. | the interaction of insulin with human circulating granulocytes was studied with the use of 125i-insulin. human granulocytes, isolated from blood by the böyum technique, showed high insulin-degrading activity in vitro which almost obscured the presence of specific, high affinity binding sites. degradation, measured by trichloroacetic acid precipitation and by binding to well characterized insulin receptors on cultured human lymphocytes (im-9 line), was due to extracellular as well as cell-bound e ... | 1976 | 4460 |
| demonstration and some properties of cytosol-binding proteins for thyroxine and triiodothyronine in human liver. | cytosol-binding proteins for l-thyroxine (t4) and triiodo-l-thyronine (t3) were studied in human liver specimens obtained at autopsy from 5 male and 2 female subjects. the liver cytosol containing 131i-t4 or t3, together with or without added stable hormones, was fractionated by pevikon thin-layer electrophoresis at ph 8.6, 8.0, and7.4. it was demonstrated in all the specimens that besides a small amount of serum t4-binding globulin, there existed three t4-binding proteins, termed ht4-1, ht4-2 a ... | 1976 | 4465 |
| glucagon-sensitive adenylate cyclase in human renal medulla. | in cell-free preparations (washed 600 x g pellets) of human renal medulla, glucagon produced a dose-dependent stimulation of adenylate cyclase. the stimulation of renal medullary adenylate cyclase by saturating concentrations of glucagon was additive to the saturating doses of vasopressin. furthermore, l-isoproterenol stimulated renal medullary adenylate cyclase in a dose-dependent manner, and this stimulation was blocked by dl-propranolol. stimulation of the renal medullary adenylate cyclase by ... | 1976 | 4466 |
| isolation of human eosinophil phospholipase d. | phospholipase d preferentially contained in human eosinophil polymorphonuclear leukocytes as compared to other leukocytes was isolated by sequential asion and cation exchange chromatography and gel filtration. the purified eosinophil enzyme specifically liberated choline from i-alpha-phosphatidyl choline with a ph optimum of 4.5-6.0 and exhibited a pi of 5.8-6.2 on polyacrylamide-gel isoelectric focusing, which are properties shared by phospholipase d from plant sources; however, its apparent mo ... | 1976 | 4468 |
| distribution and removal of cadmium from milk. | distribution of added cadmium in milk systems and the feasibility of removing cadmium were investigated. in milk containing 1 ppm cadmium, 96% of the added cadmium chloride was dispersed in the skim milk fraction, and 3% was associated with the cream fraction. cadmium was not bound strongly to any protein fraction. the association of cadmium with acid casein, whey proteins, and the fat globule membrane was 18,6, and .5% of total added cadmium. with a 5-min exposure thiosuccinylated amino-ethyl c ... | 1976 | 4479 |
| liquid-solid chromatographic determination of 6-demethylgriseofulvin in urine. | a specific and quantitative liquid-solid chromatographic method for the determination of 6-demethylgriseofulvin in human urine is reported. the method consists of extraction into an organic solvent, addition of internal standard, and analysis by liquid-solid chromatography using a uv detector. griseofulvin, if present, can be determined simultaneously. the sensitivity of the method is 6 mug/ml of urine. total 6-demethylgrisefulvin is determined after hydrolysis of the glucuronide conjugate with ... | 1976 | 4603 |
| mosquitoes of the kano plain, kenya. i. results of indoor collections in irrigated and nonirrigated areas using human bait and light traps. | | 1975 | 4620 |
| preliminary characterization of the acetylcholine receptor in human erythrocytes. | the response of human erythrocytes to cholinergic ligands was studied with an electron spin resonance assay. the membrane response to carbamyl choline was found to be antagonized by atropine and, in the absence of calcium, by tetrodotoxin. experiments with resealed ghosts showed that the membrane response to carbamyl choline required atp and calcium. reductive alkylation of intact cells eliminated the cholinergic response, but the presence of saturating amounts of carbamyl choline protected the ... | 1976 | 4659 |
| the relationship between alcohol dosage and performance decrement in humans. | the effects of alcohol on human perceptual, cognitive and motor performance was assessed in a battery of tests, and the dose-response relationships for alcohol, important for the study of drug-alcohol interactions, established. | 1976 | 4663 |
| autoregulatory system of insulin degradation in liver. ii. relationship between blood insulin levels and gsh-dependent insulin degrading activity in liver and blood. | an autoregulatory system of insulin degradation in the liver in which the rate of insulin metabolism changes in response to fluctuation in its blood levels, was investigated. in the plasma of rats and man in the absence of reduced glutathione (gsh), insulin degradation was not observed, but when a sufficient amount of reduced glutathione was added, the plasma did degrade insulin. this gsh-dependent insulin degrading activity in plasma was quite similar to that in liver in its nature. in rats, th ... | 1976 | 4693 |
| age-related changes in the content and characteristics of glycosaminoglycans in human aortas. | the vascular wall is rich in glucoseaminglicanes (gag), which form the basic intercellular substance and determine its multiple functions. using analytic and chromatographic methods the author examined hexauronic acid (ha), pentoses (p), sulphate groups and various derivatives of gag (hyaluronic acid (ha), pentoses (p), sulphate groups and various derivatives of gag (hyaluronic acid (ha), dermatan-sulfate (ds), keratansulfate (ks), chondroitinsulfate (hs), and cheransulfate (hes) in human aorta, ... | 1975 | 4292 |
| human platelet glucose-6-phosphate dehydrogenase. total purification, kinetic studies and relationship with enzyme from other blood cells. | human platelet g-6-pd has been highly purified, to homogeneity, and its kinetic, electrophoretic and immunological characteristics have been studied. platelet g-6-pd differs from erythrocyte or leukocyte enzymes by an increased michaelis constant for g-6-p and a slow activity at the acid phs. by electrofocusing only a main active band (band a) of platelet g-6-pd was found. the incubation at 37 degrees c in the presence of nadp+ and dithiothreitol normalize km-g-6-p of platelet g-6-pd; the incuba ... | 1976 | 4299 |
| acetylcholine in human muscle. | | 1976 | 4804 |
| nitrite, nitrosamines, and cancer. | carcinogenic n-nitroso compounds are formed from the reaction of naturally-occurring amines and nitrites that may be added to foods or produced by bacterial reduction of nitrate. n-nitroso compounds can be produced during processing, storage and preparation of foods and in the mammalian stomach. factors that influence the rates of nitrosation reactions include ph, temperature, catalysts, and inhibitors. predictions of the extent of nitrosation are complicated by these factors and ultimately the ... | 1976 | 4342 |
| kininogenic activity of human placenta. | | 1976 | 4360 |
| activity of two streptococcus mutans bacteriocins in the presence of saliva, levan, and dextran. | the extracellular dextrans produced from sucrose by streptococcus mutans strains bht and gs-5 did not prevent the synthesis or release of active bacteriocins by these two strains. in addition, several streptococci that were genetically sensitive to these bacteriocins, and that could synthesize a variety of extracellular dextrans and levans from sucrose, remained phenotypically sensitive when grown in the presence of sucrose. bacteriocin activity was not altered by treatment with high-molecular-w ... | 1976 | 4376 |
| crossed immunoelectrophoresis of sperm antibodies in human serum and cervical mucus. | sperm agglutinating antibodies are purified from sera and cervical mucus of women with unexplained causes of infertility which were positive in the fd-test. fractionation was performed by affinity-chromatography in a batch device and the sperm agglutinating activity controlled by the franklin and dukes test. this sperm antibody fraction was determined via crossed immunoelectrophoresis by migration into an anti-human serum containing gel. in all cases only one big peak resulted. the negative cont ... | 1975 | 4387 |
| a modified inactivation-inhibition method for determining the serum activity of alkaline phosphatase isoenzymes. | a procedure using heat inactivation and l-phenylalanine inhibition to quantitate the activities of bone, liver and intestinal alkaline phosphatase isoenzymes in human serum was confirmed by alkaline phosphatase isoenzyme analysis using an electrophoretic procedure. the results of this assay were compared with the radionuclear 85sr test, and gamma-glutamyl transpeptidase activity in a group of patients with hepatobiliary and bone diseases. | 1976 | 5210 |
| a sensitive automated colorimetric method for the determination of serum gamma-glutamyl transpeptidase. | a highly sensitive and accurate automated method using a technicon autoanalyzer aaii was developed for the determination of human serum gamma-glutamyl transpeptidase. gamma-l-glutamyl-p-nitroanilide added as substrate was split to p-nitroaniline and the gamma-glutamyl group was trapped by glycylglycine in ammediol/hc1 buffer solution. liberated p-nitroaniline was diazotized and converted by tsuda reagent (n,n-(diethyl)-n'-(1-naphthyl) ethylendiamine) to an azo-dye possessing an absorbance maximu ... | 1976 | 5211 |
| standardization in human cytogenetics. | | 1975 | 5250 |
| pituitary gonadotropin response to luteinizing hormone-releasing hormone (lh-rh) in males with azo- and oligospermia. | twenty-one males with azo- or oligospermia presenting with infertility and with no evidence of organic disease were studied with luteinizing hormone - releasing hormone (lh-rh). a pituitary luteinizing hormone (lh) and follicle stimulating hormone (fsh) response was present in all cases and was normal in the majority of the subjects. serum testosterone and 17 beta estradiol levels were normal in all cases studied. no significant correlations were found between the gonadotropin estimations and sp ... | 1975 | 4378 |
| galvanic separation of x- and y-bearing human spermatozoa. | | 1975 | 4379 |
| juvenile diabetes and human sperm quality. | spermiological findings in 25 juvenile diabetics in the age range 16 to 22 years (average 18.5) have been compared with normal spermiological findings in 24 individuals of the same age (16 to 22 years, average 18.7). a drift to lower sperm values in the group of the juvenile diabetics became evident in all parameters observed. as concerns motility and morphology, the differences between the groups reached statistically significant values. | 1975 | 4382 |
| on round-headed human spermatozoa. | in an infertile man and his only, likewise infertile, brother the sperm contained only round-headed spermatozoa. electron microscopic examination of embedded ejaculate revealed a malformation, viz. absence of acrosomes in all the spermatozoa with consequent lack of normal development of the heads of the spermatozoa. acrosomeless spermatozoa were also found in the testicular tubuli. no disturbance of the endocrine functions could be demonstrated. the blood cells were of normal male karyotype. inv ... | 1975 | 4383 |
| thiopental anaesthesia and the acid-base balance in the blood of experimental dogs. | an analysis of basic parameters representative of the acid-base balance was made in arterial blood samples from 140 clinically healthy dogs, under a general intravenous thiopental anaesthesia. the following mean values +/- s.e.m. were obtained: ph = 7.33 +/- 0.01; pco2 = 47.16 +/- 0.95; base excess = -2.12 +/- 0.27; buffer base = 46.63 +/- 0.37. the results showed a prevalent trend of lower values of ph, base excess and buffer base and higher values of pco2 than those found commonly in human cli ... | 1976 | 4849 |
| nucleotide pyrophosphatase and phosphodiesterase i. demonstration of activity in normal serum, and an increase in cholestatic liver disease. | nucleotide pyrophosphatase activity with uridine diphosphoglucose and dephospho-coa as substrates was demonstrated in normal human serum. the enzyme has a ph-optimum of about 9.6 and is inhibited by edta. phosphodiesterase i (hydrolysis of thymidine-5'-monophospho-p-nitrophenylester) was also found in normal human serum, with a ph-optimum of about 9.8 and a km of 0.20-0.25 mm. probably both activities should be attributed to one enzyme. different isoenzymes may exist, however. the activity of nu ... | 1976 | 4880 |
| some studies of beta-receptor blocking drugs on the central nervous system in man. | | 1975 | 4890 |
| the reaction of chorion epitheliums of human placentae to beta-sympathocomimetics (author's transl). | the enzyme histochemical response of chorionic epithelium after treatment with dilatel has been examined on 50 placentae of classified eutrophic premature and full-term births. in relation to the degree of damage to trophoblasts at the start of treatment and to dosage the response is mobilisation and further differentiation of langhans cells and their transformation into mature plasmodium. the relevance of these results for metabolic transport and hormonal synthesis in the feto- maternal metabol ... | 1976 | 4930 |
| impact of alfatesine anesthesia on cerebrospinal fluid pressure in man. | the variations in pressure of the cerebrospinal fluid was studied in 20 patients before and after induction of anesthesia with alfatesine (0.1 ml/kg). 14 patients received no other complementary drug (group 1); in the six other cases, 1 g. of acetylsalicylic acid was administered as an analgesic complement (group ii). all of the patients spontnaeously ventilated an o2 - n2o 50 p. 100 mixture. the c.s.f. pressure fell by 39 p. 100 on the average (p. less than 0.001) in group i and did not vary in ... | 1975 | 5009 |
| hemodynamic effect of enflurane in man. | the following hemodynamic parameters: cardiac frequency, peripheral arterial pressure, pulmonary pressure and cardiac output were measured by direct catheterisation, as the total peripheral vascular resistance and the systolic ejection volume were calculated from the registered results. the cardiac frequency and the pulmonary arterial pressure were practically not modified in our patients, though we have observed a statistically significant decrease of systolic (-30p. 100) and diastolic (-27p. 1 ... | 1975 | 5016 |
| effect of alpha and beta adrenergic blockade on epinephrine induced pulmonary insufficiency. | recent studies demonstrated that epinephrine causes significant pulmonary a-v shunting. this study reports the effect of alpha and beta adrenergic blockade on this shunting. sixty-three anesthetized mongrel dogs were ventilated with a mechanical respirator. measurements of (1) the pulmonary shunt, (2) cardiac output, (3) mean pulmonary artery, pulmonary capillary wedge and systemic pressures, and (4) pulmonary and systemic vascular resistances were obtained at 5, 15 and 30 minute intervals durin ... | 1976 | 5061 |
| dirofilaria immitis (dog heartworm) as a pulmonary lesion in humans. | dirofilaria immitis, the dog heartworm, has been identified in the pulmonary granulomas of 5 patients from the greater charleston area; this is the largest series of such cases from one medical center. the patients had no pulmonary symptoms. on roentgenogram the lesions were all about 2 cm in size, of uniform light opacity, and located near the pleural surface. thoractomy was performed in each instance because of the possiblity of carcinoma. the association of granuloma formation, pulmonary infa ... | 1976 | 5063 |
| human tear ph. diurnal variations. | using a closed chamber microelectrode system, the tear ph levels of sixteen subjects were monitored throughout the waking extent of five days. in addition to the absolute mean ph differences found among subjects, diurnal patterns of ph change could be identified for the majority. the amplitudes and periods of these cycloid patterns, however, were distinctive to each individual. also, tear ph levels following periods of prolonged eye closure were found to be notably more acid than those associate ... | 1976 | 5068 |
| human granulocyte ribonuclease. | | 1976 | 5077 |
| inhibition of atpase activity in human red cell membranes by tetracaine. | | 1976 | 5091 |
| the major sialoglycoprotein of the human erythrocyte membrane. release with a non-ionic detergent and purification. | the major sialoglycoprotein of the human erythrocyte membrane has been selectively released by the non-ionic detergent tween 20 and further purified in detergent-free buffers by hydroxyapatite chromatography and, finally, by hydrophobic interaction chromatography on pentyl-sepharose. the purified glycoprotein shows one main zone, pas-1, and up to three minor zones after staining both for protein and carbohydrate in polyacrylamide gel electrophoresis in the presence of dodecyl sulfate. the relati ... | 1976 | 5123 |
| spectroscopic studies on the complex formation of suramin with bovine and human serum albumin. | the binding of suramin to bovine and human serum albumin was investigated by gel filtration and spectroscopic measurements. besides some low-affinity binding sites suramin has, on the bovine serum albumin molecule one and on the human serum albumin molecule two, high-affinity binding sites. spectroscopic measurements reveal that there are large differences between the albumins in the mechanism of binding to the high-affinity binding sites. further, it is suggested that high concentrations of sur ... | 1976 | 5125 |
| interaction of serum albumin with normal and sickle hemoglobins. | the stability of oxyhemoglobin s during mechanical shaking was enhanced by the addition of human serum albumin. the stabilizing effect was maximum when the concentration of serum albumin approached that of oxyhemoglobin, suggesting a molecular level interaction between them. the effects of serum albumin on oxyhemoglobin a were essentially similar to those on oxyhemoglobin s. deoxy- and methemoglobins were also stabilized by serum albumin. the addition of human serum albumin to a solution contain ... | 1976 | 5129 |
| a phospholipase a2 with anticoagulant activity. i. isolation from vipera berus venom and properties. | an anticoagulant protein has been isolated by deae cellulose chromatography and gel filtration from the venom of the vipera berus orientale (eastern europe). purification has been completed by elution on carboxymethyl cellulose with continuous gradient at constant ph. the inhibitor of coagulation was separated from the other venom enzymes, e.g. procoagulant, fibrinogenolytic, aminoesterase and amino acid oxidase activities. it was also separated from other phospholipase components which were not ... | 1976 | 5142 |
| terrestrial vertebrates of the new hebrides: origin and distribution. | the known terrestrial vertebrate fauna of the new hebrides consists of 16 species of mammals (excluding feral domestic stock), 61 species of resident land- and freshwater birds, 20 species of reptiles and one amphibian. of these, three, five, four and one species respectively have apparently been introduced by man. the non-introduced fauna is clearly indo-australian in origin, but some species have an exclusively pacific island distribution and others (two bats, seven birds, and four lizards) ar ... | 1975 | 4831 |
| the effects of dopamine and isoproterenol on the pulmonary circulation. | dopamine and isoproterenol, although used primarily for their inotropic effects, are also potent vasoactive substances. to determine their effects on the pulmonary circulation, we cannulated the left lower lobe bronchus in 20 dogs to permit ventilation with either air or a mixture of 95% nitrogen and 5% carbon dioxide; systemic oxygenation was maintained by venitlating the right lung with 95% oxygen. the lobe was perfused at a controlled flow rate and left atrial pressure was held constant. hypo ... | 1976 | 5634 |
| biochemistry of copper. | copper, as a component of numerous cuproenzymes, plays a vital role in many physiologic functions in man and animals. from the stand-point of human health there are at least three functional areas of prime importance. copper is involved in the development and maintenance of cardiovascular and skeletal integrity, central nervous system structure and function, and erythropoietic function including iron metabolism. although there is no evidence for widespread copper deficiency in the human populati ... | 1976 | 5635 |
| study of cystinaminopeptidase heterogeneity in animal sera by the isoelectric focusing method. | study of the quantitative distribution of cystinaminopeptidase activity in the serum of pregnant and control animals (cow, sheep and rat) by the isoelectric focusing method showed heterogeneity of the cystinaminopeptidase activity of the animals' serum enzymatic system, which was well pronounced in the cow and rat and less pronounced in the sheep. the authors assume from their results that the principal difference between the pregnant and control animals is not the total increase in the level of ... | 1976 | 4848 |
| national health planning. what scares the hell out of gene rubel? | | 1976 | 5660 |
| copper (ii) induced polymerization of human albumin, and its depolymerization by diglycyl-l-histidine: a ph static and ultracentrifugation study. | copper (ii) ions successively induce dimers and tetramers of human serum albumin (l) when the cu (ii) concentration is extended beyond that of 200 mum. this is shown by emf titrations and by ultracentrifugation experiments. the emf titrations, which involve a new ph static method, were performed at 25 degrees, in a 0.5 m nacio4 medium at ph 6.59, using glass and copper amalgam electrodes. the total concentration of cu(ii) varied from 0.14 to 2.2 mm and the albumin concentration from 0.05 to 0.7 ... | 1975 | 5157 |
| fluoride incorporation into powdered human enamel under conditions of fluctuating ph. | | 1976 | 5192 |
| vasodilator administration in the presence of beta-adrenergic blockade. | to explore the possibility that the presence of propranolol-induced beta-adrenergic blockade might have an adverse effect upon homeostatic circulatory reflexes activated by the administration of a potent vasodilator agent, arterial blood pressure and pulse rate response to rapid intravenous diazoxide injection was monitored before and after pretreatment with propranolol in ten hypertensive patients. it appeared that beta-adrenergic blockade had no clinically significant effect on the magnitude o ... | 1976 | 5209 |
| qualitative metabolic fate of phenoxybenzamine in rat, dog, and man. use of 15n-labeling. | administration of an equimolar mixture of unlabeled and 15n-labeled phenoxybenzamine to rats and dogs facilitated identification of urinary metabolites by gas chromatography/chemical-ionization mass spectrometry by virtue of the the conspicuous equal-intensity ion pairs produced. by use of this technique n-benzyl-n-phenoxyisopropylamine (iii), n-benzyl-n-(p-hydroxy-phenoxyisopropyl)amine (iv), and 2-benzylamino-1-propanol (vi) were identified as metabolites in rats. phenoxyisopropylamine (v) as ... | 1976 | 5261 |
| levamisole, a chemically defined substance with immunostimulating potential--a review. | in contrast to immunosuppression, immunostimulation is still at an early stage of development. levamisole is the first chemically defined substance with immunostimulating potential which can be applied systemically. after a short consideration of the chemistry and pharmacology of levamisole, its effect in different animal experimental models of humoral and cell-mediated immunity are discussed in detail. moreover, the following review deals with the influence of levamisole on several immunologica ... | 1976 | 5347 |
| isolations in a mosquito (aedes pseudoscutellaris) cell line (mos. 61) of yellow fever virus strains from original field material. | a simple, rapid and inexpensive method of isolating yellow fever (yf) virus from naturally infected mosquitoes, human liver and the serum of a sentinel monkey by inoculation of a continuous line of mosquito cells is described. the mosquito cells were more sensitive than suckling mice and marginally better than vero cells for primary isolation. this is the first time that mosquito cells have been successfully used for primary isolation of yf virus from field material. | 1975 | 5385 |
| spot hemoglobin. studies on the root effect hemoglobin of a marine teleost. | the spot, leiostomus xanthrus, has a single tetrameric hemoglobin. structural studies indicate the presence of alpha- and beta-like chains with cooh-terminal sequences of --arg and --tyr-his, respectively, the same as is found in human hemoglobin. spot hemoglobin possesses a root effect: a heterotropic control mechanism like the bohr effect but with more extreme ph dependence in the equilibria and kinetics of o2 and co binding. the root effect seems to be a molecular adaptation, in that ph- and ... | 1976 | 5433 |
| site-site interactions among insulin receptors. characterization of the negative cooperativity. | by studying the dissociation of 125i-instulin from its receptors in the absence and phe negatively cooperative type for the insulin receptors. in the present study we extend oy purified mouse and rat liver membranes as well as in human circulating monocytes and human cultured lymphocytes demonstrated negative cooperativity that was extraordinarily simn membranes more slowly than it does from its receptors on whole cells. the dissociaty a small percentage of the receptor sites (1 to 5%), are suff ... | 1976 | 5434 |
| kinetic study of the action of snake venom phospholipase a2 on human serum high density lipoprotein 3. | the hydrolysis of the phospholipids of intact human serum high density lipoprotein 3 (hdl3) by pure alpha-phospholipase a2 from crotalus adamanteus was studied by ph-stat titration. the enzyme quantitatively hydrolyzed phosphatidylcholine and phosphatidylethanolamine and left sphinogomyelin intact, yielding a stable and water-soluble modified hdl. lysophospholipids and free fatty acids, the products of hydrolysis, remained in the lipoprotein. when 1 mol of defatted bovine serum albumin/mol of su ... | 1976 | 5436 |
| pulse radiolytic investigation of single heme group reduction in hum an methemoglobin. | reduction of one of the four heme groups of human aquomethemoglobin a has been investigated by the pulse radiolysis method. the reactivity of e-a-q, the hydrated electron, with methemoglobin was determined by observing this species directly. the separate reactions of the hydroxy yl radical and hydrogen atom, as well as of e-a-q, were studied by observing absorbance changes in the protein spectrum over the wavelength range 290 to 600nm, with appropriate scavengers in solution... | 1976 | 5439 |
| beta2-adrenoceptors facilitating noradrenaline secretion from human vasoconstrictor nerves. | isolated biopsy specimens of human peripheral arteries and veins, preincubated with 3h-(-)- noradrenaline (na) to label the neural stores of na, were used to study the beta-adrenoceptors previously found to increase the secretion of 3h-na evoked by electrical field stimulation of the adrenergic nerves of this tissue. the increase in nerve stimulation induced secretion of 3h-na caused by 0.04 mum isoprenaline was prevented by 1 mum propranolol. this beta-blocking drug by itself slightly but signi ... | 1976 | 5851 |
| genetic variants of human erythrocyte glucose-6-phosphate dehydrogenase. kinetic and thermodynamic parameters of variants a, b, and a- in relation to quaternary structure. | the values of vmax and km for the three genetic variants a, b, and a- of erythrocyte glucose-6-phosphate dehydrogenase have been determined at 10 different ph values in the range from 5.5 to 9.5, and at four different temperatures in the range from 18.5-40.0 degrees. the log vmax versus ph curve for each of the enzymes shows a monotonic increase between ph 5.5 and 7, and a plateau from ph 7.5 upwards. these curves, and their temperature dependence, are compatible with the presence of a single io ... | 1976 | 5448 |
| levels of immunoglobulins and antibodies to haemaglutinin and neuraminidase of influenza virus in nasal secretions after natural infection. | nasal washings (nw) from 16 influenza patients in the course of an epidemic in november and december, 1974 were examined for the presence of influenza virus, immunoglobulins (ig) and titres of haemagglutination inhibiting (hi) and neuraminidase inhibiting (ni) antibodies. influenza virus identical with a/port chalmers/1/73 (h3n2), increased levels of iga and occasionally igg, and specific antibodies were detected in the nw. the dynamics of hi and ni antibody formation did not differ substantiall ... | 1976 | 5867 |
| associated seroconversions to respiratory viruses in volunteers with experimental influenza infection. | serological examinations of 573 volunteers with mild experimental influenza infection and 86 volunteers of a control group hospitalized in a special clinic revealed a significant rise in the titre of antibodies (seroconversion) not only to influenza a or b viruses used for the experimental infection but in 23.3 to 29.8% of cases also to other respiratory viruses. based on a number of arguments, associated seroconversions are interpreted as due to mixed or sequential infections of different aetio ... | 1976 | 5868 |
| electron microscopy of virus particles in ultrathin frozen sections. | a procedure with the use of polyethylene glycol (peg) 600 to support biological materials for ultracryotomy was developed. developing and mature virions were demonstrated by electron microscopy in negatively stained frozen sections of vaccinia virus-infected human diploid cells and of herpes simplex virus (hsv) type 1 infected primary rabbit kidney cells. approximate localisation of the virus particles in the cells was possible. | 1976 | 5872 |
| interferon pretreatment primes interferon production by human adenovirus in chick embryo cells. | pretreatment of chick embryo cells (cec) with homologous interferon enhanced the subsequent interferon production induced by human adenovirus. this priming effect of interferon pretreatment was also demonstrable when trypsin-treated virus was used for the induction of interferon. | 1976 | 5873 |
| demonstration of influenza virus a in human heart by semiquantitative virus assay and immunofluorescence. | | 1976 | 5876 |
| the interaction of macromolecular solutions with macromolecular monolayers adsorbed on a hydrophobic surface. | in order to elucidate the general patterns of intermacromolecular surface interactions that may be involved in hemocompatibility phenomena, monolayers of representative macromolecules on an octadecylsilylated glass surface were exposed to solutions of other macromolecules, and the changes in interfacial composition were characterized by zeta potential-ph titration curves, as measured by alternating flow streaming current analysis and, in some cases, by radiotracer labeling. experiments with poly ... | 1976 | 5457 |
| stimulation of ornithine decarboxylase activity in chick fibroblasts by non-suppressible insulin-like activity (nsila), insulin and serum. | a factor isolated from human serum (nonsuppressible insulin-like activity, nsila) stimulates multiplication of serum-starved chick embryo fibroblasts and stimulates activity of ornithine decarboxylase (odc). physiological doses of nsila (200 muu/ml) and pharmacological doses of insulin (200 mu/ml) stimulate odc 4-5-fold, 10% fetal calf serum about 18-fold. combined addition of nsila and insulin does not result in higher activities, suggesting a common mechanism of action. the increase in cell nu ... | 1976 | 5458 |
| gas-liquid chromotographic determination of perazine, thioridazine and thioridazine metabolites in human plasma. | a gas-liquid chromatographic method for the detection of perazine, thioridazine and its major metabolites in human plasma is presented. repeated extraction, an internal standard and a temperature program with flame ionization detection make possible accurate and reproducible results with patients on therapeutic doses of these drugs. examples of chromatograms after extraction of plasma are given. | 1976 | 5459 |
| lysosomal enzyme secretion from human neutrophils mediated by cyclic cmp: inhibition of cyclic gmp accumulation and neutrophil function by glucocorticosteroids. | the effects of several glucocorticosteroids on cyclic gmp accumulation, guanylate cyclase activity, calcium influx, lysosomal enzyme secretion, and phagocytosis were studied in human neutrophils. contact between neutrophils and serum-treated zymosan particles, in the presence of calcium at ph 7.4, triggered these cellular events within five minutes. each of these neutrophil functions was markedly inhibited by methylprednisolone sodium succinate, triamcinolone acetonide hemisuccinate and parameth ... | 1975 | 5468 |
| ontogenetic development of nadh-dependent methemoglobin reductase in erythrocytes of man and rat. | ontogenetic development of nadh-dependent methemoglobin reductase was followed in humans and rats. the human kinetic profile differs from that in the rat. the low level of methemoglobin reductase in human infants at birth and for the first months of life may provide a partial explanation of the particular susceptibility to methemoglobinemic agents of this age group. | 1976 | 5611 |
| structural studies of human chorionic gonadotropin and its subunits using tyrosine fluorescence. | | 1976 | 5960 |
| cardiorespiratory response to feeding in newborn infants. | milk feeds were given through indwelling nasogastric tubes to 14 infants with respiratory distress. similar cardiorespiratory disturbances were observed when the infants were fed (5 ml/kg per feed) human milk, cow's mild, or distilled water. pao2 fell after a feed but recovered to the prefeed value at 30 minutes, at which time paco2 had fallen and the respiratory rate had increased. no changes in ph, heart rate, or blood pressure were observed. portal sinus pressures rose after feeding in assoc ... | 1976 | 5964 |
| investigations on the function of the rat forestomach. | the functions of the rat forestomach and upper digestive tract were studied. the ph values, alpha-amylase activity, quantitative estimates of microorganisms, and emptying rates were higher in the forestomach than in the glandular stomach. rats with surgically removed forestomachs lived without complications for more than 1 yr. their alimentary hyperglycemia was higher and shorter than in controls. the significance of rich microflora present in the conventional forestomach is not known, although ... | 1976 | 5626 |
| inhibition of mn2+ of citrate supported progesterone biosynthesis in mitochondrial fractions of human term placentae. | | 1976 | 5631 |
| on the mitochondrial 17beta-hydroxysteroid dehydrogenase from human endometrium and endometrial carcinoma: characterization and intramitochondrial distribution. | | 1976 | 5632 |
| hew hangs tough on planning. | | 1976 | 5659 |
| dual effect of 2,3-diphosphoglycerate on the bohr effects of human blood. | the influence of the red cell concentration of 2,3-diphosphoglycerate (2,3-dpg, 0.5-26 mumoles/g erythrocytes) on the "co2-bohr effect" (ph varied by co2 at constant base excess) and the "fixed acid-bohr effect" (ph varied by fixed acid or base at constant pco2) was studied in human blood at plasma ph values ranging between ph 7.2 and ph 7.6. elevation of red cell 2,3-dpg concentration leads to a numerical decrease of the "co2-bohr coefficient" referring to plasma ph. the "fixed acid-bohr coeffi ... | 1976 | 5701 |
| influence of 2,3-diphosphoglycerate on the buffering properties of human blood: role of the red cell membrane. | the effect of the concentration of red cell 2,3-diphosphoglycerate (2,3-dpg, 0.5-21 mumoles/g cells) on the buffering properties and on the slope of the relation between the extracellular and intracellular ph (deltaphi/deltaphe) of human blood was studied. the results were evaluated in connection with previous findings concerning the effect of 2,3-dpg on the donnan ratio rh+ = h+e/h+i. deltaphi/deltaphe decreases with rising red cell 2,3-dpg content as well as with rising extracellular ph. delta ... | 1976 | 5702 |
| differential dependence on nadph concentrations of two microsomal corticosteroid 21-hydroxylation reactions. | the dependency on nadph concentrations of the bovine adrenal cortex microsomal 21-hydroxylation of progesterone and of 17-hydroxyprogesterone was investigated. the average km for nadph in the 21-hydroxylation of progesterone is 10.4 mum while that for the 21-hydroxylation of 17-hydroxyprogesterone is 0.6 mum. the optimal nadph concentrations for these two hydroxylations are, in average, one order of magnitude apart. the different affinities of the two 21-hydroxylating activities with respect to ... | 1976 | 5267 |
| arylamidases in normal and diseased human muscle. | human skeletal muscle homogenates were found to contain enzymes that catalyze the hydrolysis of beta-naphthylamides of leucine, arginine and lysine, known substrates for neutral and basic arylamidases. they also contained a trace of activity towards alpha-aspartyl-beta-naphthylamide. the muscle arylamidases were found to be inhibited by p-chloromercuribenzoate, hg2+ and puromycin. leucyl, arginyl and lysysl arylamidases were slightly activated by cobalt ions. when compared to controls, no signif ... | 1976 | 6175 |
| surgical aspects of infertility. | these surgical procedures that have been used in the management of male infertility have recently been subjected to critical review. in a recent study (getzoff, 1973) designed to summarise the experience of 150 urologists in the surgical treatment of male infertility, several valuable viewpoints were revealed. 1. there is a need for establishing criteria for labelling an operation as a 'success'. in this group, 42 per cent defined a successful operation as the postoperative appearance of a norma ... | 1975 | 6178 |
| neutral proteinase activity in the human lens. | | 1976 | 5278 |
| lack of relationship between cyclic nucleotide levels and spermatozoal function in human semen. | cyclic adenosine 3':5'-monophosphate (cyclic amp) levels were determined in 103 samples of human semen and grouped according to the number of spermatozoa in the ejaculate. no correlation was found between cyclic amp concentrations and the number, motility, and morphology of the spermatozoa or the fructose content, ph, and volume of the ejaculate. similar findings were obtained with cyclic guanosine 3':5'-monophosphate levels in 24 samples of human semen. therefore, cyclic nucleotide levels in hu ... | 1976 | 5322 |
| description of new mutant forms of erythrocyte glucose-6-phosphate dehydrogenase in man. | 9 variants of human erythrocyte glucose-6-phosphate dehydrogenase (g6pd) were isolated from erythrocytes of patients with g6pd deficiency and partially purified according to who program for stanartization of methods for studying g6pd. the results of physico-chemical study of these enzymes (determination of electrophoretic mobility, kappam for g6p and nadp, ph optimum and thermostability) permit tu consider 5 of them to be new mutations of g6pd previously not described in literature. the observed ... | 1975 | 5339 |
| ethical limits of the maintenance of life. | | 1976 | 5750 |
| dependence of csf on plasma bicarbonate during hypocapnia and hypoxemic hypocapnia. | we have previoulsy shown ph compensation to be similar in csf and arterial blood during chronic hypoxemic hypocapnia in man and pony, and postulated that the compensatory reduction in csf hco3 was dependent upon corresponding changes in hco3a. we tested this hypothesis in anesthetized, paralyzed dogs by determining the effects of 7 or 14 hours of hypocapnia (paco2 20 and 30 mm hg), hypoxemia (pao2 30, 38 and 48 mm hg) and hypocapnic hypoxemia on csf acid-base status. hco3a was either permitted t ... | 1976 | 5765 |
| response to jejunal acidification in man. i. changes in composition of perfusate. | the jejunal disposal of perfused acid was studied in 11 control subjects and 12 patients with duodenal ulcer. it was found that the capacity to dispose of acid was saturable and was less than control in patients with duodenal ulcer. when the load of acid was small, all the acid disappeared from the jejunum and sodium bicarbonate could be aspirated. during acid perfusion there was secretion of water and electrolytes into the jejunum if the load of acid exceeded the dissipative capacity. superimpo ... | 1976 | 5770 |
| the hydrolysis and absorption of conjugated folates in man. | after ingestion of synthetic pteglu3 in physiological doses, the folate forms were studied in plasma obtained from human vena portae blood. plasma conjugase was inhibited by rapid heat-denaturation. the folate forms were assayed with l. casei and s. faecalis and identified by thin-layer chromatography (biautography). mono- and diglutamic folate was demonstrated in the portal plasma, indicating that intestinal hydrolysis precedes the absorption of conjugated folates. the pteroylpolyglutamate hydr ... | 1976 | 5771 |
| abnormal liver enzymes and human chorionic gonadotropin elevation in abdominal testicular seminoma. | a case of seminoma in an abdominal testis is presented in which abnormal liver enzyme as well as elevated human chorionic gonadotropin levels were present which returned to normal after surgical extirpation of the primary lesion. a review of pertinent literature and implications of these unusual findings are presented. | 1976 | 5796 |
| tyrosine uptake and neurotransmitter synthesis in ischemic brain after administration of alpha and beta adrengeric blocking agents to man. | | 1975 | 5798 |
| the electrolyte pattern of the gastric secretion from the vervet monkey. | the output of acid, chloride, sodium and potassium in gastric washings from the vervet monkey have been measured under basal conditions and while stimulating the secretion with two doses of pentagastrin (one dose submaximal, the other dose supramaximal). the output during washings with different weak electrolyte solutions (5 meq/1 nac1, kcl, or hcl or 0.36 m glycine) are compared. the results show that the composition of the secretion was independent of the composition of the rinsing fluid in th ... | 1975 | 5809 |
| bile canalicular alkaline phosphatase and disease. | the alkaline phosphatase reaction is normally absent in human bile canaliculi, but was found in 79 patients. in search for a common causal factor, these patients were further examined. thirty-seven were autopsied. the conditions most ocmmonly associated with the phenomenon were malignant tumours with or without involvement of the liver, collagen diseases, long-standing partial obstruction of the common bile duct, and genetic variants of alpha-1-antitrypsin. no clinical or laboratory facts were c ... | 1976 | 5842 |
| interferon-producing capacity of human tonsil cells and properties of interferon produced by these cells. | tonsils excised from human beings for chronic tonsillitis have been shown to be an accessible and sufficiently rich source of human lymphocytes. tonsil cells produced interferon as intensively as blood leukocytes and the properties of this interferon were similar to those of leukocyte interferon. the optimal conditions for interferon production by tonsil cells were established. | 1976 | 5865 |
| mediators of the allergic reaction. slow reacting substance (srs-a). | the slow reacting substance of anaphylaxis (srs-a) belongs to a group of substances which produce a slow progressive and sustained contraction of some smooth muscles. it is released by the interaction of the antigen with certain antibodies; in humans through the interaction with the ige or reagine. the srs-a is a heat-labile sustance, chemically unstable, of an acid character, with a low molecular weight. it is not destroyed by the action of proteolytic enzymes. its molecular structure has not y ... | 1975 | 5879 |
| breathing patterns before death in fetal lambs. | continuous observations in 16 fetal lambs over many days before death in utero from hypoxia, asphyxia, infection, and other causes have shown that death is preceded by variable changes in breathing movements. there was always a prolonged period of apnea succeeded by continuous abnormal breathing in six lambs and by gasping or brief episodes of abnormal breathing in the remainder. these abnormal patterns are described as a warning of what may be encountered in the human fetus. | 1976 | 5894 |
| enzyme system and coenzymes involved in the energy metabolism of leukocytes. function and metabolism of polymorphonuclear neutrophils. | mitochondria may be isolated from various types of leukocyte (neutrophil polymorphs and lymphocytes from human blood, neutrophil polymorphs and macrophages from peritoneal exudates of the guinea pig) after destruction by heparin of the cell membrane. this procedure is very simple and less traumatic for these subcellular structures than the usual mechanical procedures. the enzyme activities of the respiratory chain and oxygen consumption may be measured in these mitochondrial preparations. the ox ... | 1975 | 5934 |