| comparative immunogenicities of chikungunya vaccines propagated in monkey kidney monolayers and chick embryo suspension cultures. | a comparative study was made of formalin-inactivated chikungunya vaccines prepared from the virus propagated in african green monkey kidney monolayers and concentrated chick embryo suspension cultures. the vaccine prepared in the chick embryo suspension cultures was significantly more protective to mice against a live homologous virus challenge and stimulated the production of 4 to 5 times more circulating antibodies than the vaccine prepared with virus grown in african green monkey kidney monol ... | 1972 | 4624213 |
| chromatin structure and the cell cycle. | pancreatic dnase i is used to probe the structure of chromatin isolated from synchronized hela cells. the degree to which dna in chromatin is protected from dnase attack varies during the g(1), s, and g(2) phases of the cell cycle. in addition, the dnase sensitivity of chromatin from contact-inhibited african green monkey kidney cells differs from that of actively dividing, subconfluent cultures. these cell cycle-dependent chromatin changes were observed consistently at all enzyme concentrations ... | 1972 | 4626402 |
| comparative features of the reproductive biology of hamadryas baboons (papio hamadryas), grivet monkeys (cercopithecus aethiops) and rhesus monkeys (macaca mulatta). | | 1972 | 4627306 |
| dna replication in synchronized cultured mammalian cells. iv. replication of african green monkey component and bulk dna. | | 1972 | 4627587 |
| replication of avian infectious bronchitis virus in african green monkey kidney cell line vero. | | 1972 | 4627930 |
| rabies antibody determination by immunofluorescence in tissue culture. | a tissue culture (tc)-fluorescent antibody (fa) technique for the measurement of rabies-neutralizing antibody was found to be reliable and comparable to the standard mouse serum neutralization test. this test was performed with bhk-21 cells infected with the era vaccine virus strain on lab-tek tc chamber slides. a flury high egg passage (hep) rabies virus strain grown on continuous line of african green monkey (vero) and on bhk-21 cells was investigated to determine its utilization in a tc-fa ne ... | 1972 | 4628958 |
| a blood-aqueous barrier to small molecules in the ciliary processes of the vervet monkey (cercopithecus aethiops). | | 1972 | 4630657 |
| survival of animal tissue cells in primary culture in the absence of serum. | the ability of cells from tissues of several species of animals to survive in primary culture without serum was tested. of the species tested, cells from the kidneys of macaca mulatta (rhesus) and cercopithecus aethiops (vervet) monkeys and chicken embryo cells not only survived under these conditions, but indeed developed into confluent monolayer cultures. the addition of either serum or its globulin or albumin fraction enhanced the development of cell monolayers and permitted those cells unabl ... | 1973 | 4631437 |
| vitamin c requirements of the vervet monkey (cercopithecus aethiops) under experimental conditions. | | 1973 | 4633567 |
| cell fusion by canine distemper virus-infected cells. | av3 cells (continuous human amnion) infected with the onderstepoort strain of canine distemper virus produced cell fusion within 2 to 5 hr when added to av3 cell monolayers. an apparent requirement for intact, infected cells was demonstrated by showing that (i) frozen-and-thawed infected cells failed to induce fusion, (ii) infected cells frozen in the presence of glycerol retained their ability to induce fusion, (iii) infected cells subjected to swelling in hypotonic buffer and homogenization lo ... | 1972 | 4644630 |
| evolutionary conservatism in arrangement of genetic material. a comparative analysis of chromosome banding between the rhesus macaque (2n equals 42, 84 arms) and the african green monkey (2n equals 60, 120 arms). | | 1973 | 4783711 |
| isolation and propagation of adenoviruses in african green monkey kidney cultures. | | 1965 | 4954392 |
| observations on the menstrual cycle of the grivet monkey. (cercopithecus aethiops aethiops) in the sudan. | | 1966 | 4955692 |
| response of cercopithecus aethiops to cholera vibrio lipopolysaccharide and psychological stress. | | 1966 | 4959386 |
| experimental schistosomiasis in primates in tanzania. i. a preliminary note on the susceptibility of cercopithecus aethiops centralis to infection with schistosoma haematobium and schistosoma mansoni. | | 1966 | 4960090 |
| cytopathic and plaque assay of rubella virus in a line of african green monkey kiency cells (vero). | | 1967 | 4961492 |
| replication of rubella virus in a continuous line of african green monkey kidney cells (vero). | | 1967 | 4961494 |
| social structure among vervet monkeys (cercopithecus aethiops). | | 1967 | 4965299 |
| isolation and characterization of simian adenoviruses isolated in association with an outbreak of pneumoenteritis in vervet monkeys (cercopithecus aethiops). | | 1967 | 4965334 |
| [on an infectious disease transmitted by cercopithecus aethiops. ("green monkey disease")]. | | 1968 | 4966281 |
| [on the neuropathology of the green monkey (cercopithecus aethiops) transmitted infectious diseases in marburg]. | | 1968 | 4966284 |
| observations on the fine structure of spermatozoa of the bush baby (galago senegalensis), the african green monkey (cercopithecus aethiops) and man. | | 1967 | 4966398 |
| the relation between the ciliary muscle and the trabecular meshwork and its importance for the effect of miotics on aqueous outflow resistance. a study in two contrasting monkey species, macaca irus and cercopithecus aethiops. | | 1967 | 4967268 |
| problems in the detection of rubella virus in african green monkey kidney tissue culture. | | 1968 | 4968540 |
| rubella hemagglutinin prepared in a serial line african green monkey kidney cells. | | 1968 | 4969859 |
| blood cells in the african green monkey. | | 1968 | 4970328 |
| [studies on the animal-oriented pathogenicity of a cercopithecus aethiops transmitted human pathogenic agent]. | | 1968 | 4971554 |
| an infectious disease transmitted by cercopithecus aethiops. ("green monkey disease"). | | 1968 | 4974180 |
| distribution of cholinesterase in the basal rhinencephalic structures of the grivet monkey (cercopithecus aethiops aethiops). | | 1968 | 4975689 |
| [adaptation and selection of a highly attenuated variant of measles virus in primary kidney cultures of cercopithecus aethiops]. | | 1967 | 4976084 |
| production of kidney tissue cultures from african green monkeys, experimentally infected with the causative agent of frankfurt-marburg-syndrome. | | 1969 | 4977816 |
| external morphology of the brain of the givet monkey (cercopithecus aethiops aethiops). | | 1968 | 4978304 |
| comparative sensitivity of tissue cultures to rubella virus: use of guinea pig cells for virus titration. | a series of 19 different primary and serial tissue cultures were investigated for their sensitivity to virulent or attenuated rubella virus (rv). primary guinea pig tissues, a serial passage of baby hamster kidney, and primary human amnion were comparable to african green monkey kidney tissue cultures in their sensitivity. in general, primary human tissues were relatively insusceptible to the gilchrist strain of rv. rv interfered with the growth of vesicular stomatitis virus. based on this findi ... | 1969 | 4979943 |
| two classes of spermatogonial stem cells in the monkey (cercopithecus aethiops). | | 1969 | 4982042 |
| biological characteristics and viral susceptibility of an african green monkey kidney cell line (vero). | | 1969 | 4982209 |
| [electron microscopical findings in a communicable disease in monkeys (cercopithecus aethiops)]. | | 1968 | 4985421 |
| [the influence of methoxychlor on tissue culture of the kidney of the monkey cercopithecus aethiops and of the lung of the human embryo]. | | 1970 | 4987182 |
| salivary amylase. ii. alpha-amylase in salivary glands of the macaca irus monkey, the cercopithecus aethiops monkey, and man. | | 1970 | 4988027 |
| contamination of simian viruses in african green monkeys (cercopithecus aethiops). | | 1969 | 4992619 |
| studies of rubella virus. 1. a study of growh of rubella viru in kidney culture cells of african green monkey. | | 1969 | 4993211 |
| characteristics of the bgm line of cells from african green monkey kidney. brief report. | | 1970 | 4993582 |
| an epithelial blood-aqueous barrier to horseradish peroxidase in the ciliary processes of the vervet monkey (cercopithecus aethiops). | | 1971 | 4994081 |
| dna strand reassociation and polyribonucleotide binding in the african green monkey, cercopithecus aethiops. | | 1971 | 4995480 |
| an electron microscopic study of the permeability of iris capillaries to horseradish peroxidase in the vervet monkey (cercopithecus aethiops). | | 1971 | 5000137 |
| subhuman primate diploid cells: possible substrates for production of virus vaccines. | results of a program for development of new cell lines suggest that it it possible to establish cell lines from both rhesus and african green monkeys which are comparable to diploid lines of human origin, and that these monkey lines should be candidates for use in the production of virus vaccines. | 1971 | 5000950 |
| play-mothering: the relations between juvenile females and young infants among free-ranging vervet monkeys (cercopithecus aethiops). | | 1971 | 5001014 |
| proposal for classifying human strain navel and related simian mycoplasmas as mycoplasma primatum sp. n. | mycoplasmas isolated from simian (cercopithecus aethiops) tissues were shown to have biological, biochemical, and serological properties and electrophoretic cell protein patterns similar to strain navel isolated from man 15 years ago. the simian and human navel strains comprised a single serogroup, distinct from the established mycoplasma and acholeplasma species of the class mollicutes. it is proposed that strains with the properties described be named mycoplasma primatum. | 1971 | 5001200 |
| heterochromatin location on chromosomes of normal and transformed cells from african green monkey (cercopithecus aethiops). dna denaturation-renaturation method. | | 1971 | 5001376 |
| paracrystal formation in cell cultures infected with adenovirus type 2. | large rod-shaped structures corresponding to paracrystals were seen in the nucleus, cytoplasm, or both of adenovirus type 2 (ad2)-infected cells by immunofluorescence staining with antibody prepared against purified ad2. in exception to this, ad2-induced crystals did not stain with either hexon or fiber antibody. the crystalline structures were first observed in ad2-infected vero cells at 28 hr with a maximum number at 70 hr postinoculation. the kinetics of paracrystalline formation closely para ... | 1971 | 5006035 |
| uptake of heterologous genome by mammalian spermatozoa and its transfer to ova through fertilization. | simian virus 40 (sv40) adsorbs on rabbit spermatozoa but does not penetrate the cells, as indicated by the absence of radioactive material seen on autoradiography of spermatozoa exposed to [(3)h]thymidine-labeled sv40. in contrast, after exposure of spermatozoa to labeled sv40 dna, radioactive material was found in the postacrosomal area of the spermatozoa. furthermore, when spermatozoa exposed to sv40 dna were fused with cells of the cv-1 line of african green monkey kidney cells, infectious sv ... | 1971 | 5277085 |
| the neuropathology of an infectious disease transmitted by african green monkeys (cercopithecus aethiops). | | 1969 | 5389368 |
| effect of ultraviolet irradiation on the survival of simian virus 40 functions in human and mouse cells. | the relative sensitivities to ultraviolet light of various simian virus 40 (sv40) functions were studied in human and mouse cells. transformation appeared to be less ultraviolet (uv)-sensitive than either t or v antigen when all functions were compared in the same cell. however, the time course of both t- and v-antigen appearance was delayed with uv-irradiated virus, so that the survival curves of these functions changed with time. mouse and human cells which were transformed by uv-sv40 all cont ... | 1970 | 5497884 |
| preliminary communication on gross pathological lesions in the genito-urinary systems of vervet monkeys (cercopithecus aethiops) experimentally infected with schistosoma haematobium. | | 1970 | 5500113 |
| deoxyribonucleic acid replication in simian virus 40-infected cells. iv. two different requirements for protein synthesis during simian virus 40 deoxyribonucleic acid replication. | the replication of simian virus 40 (sv40) deoxyribonucleic acid (dna) was inhibited by 99% 2 hr after the addition of cycloheximide to sv40-infected primary african green monkey kidney cells. the levels of 25s (replicating) and 21s (mature) sv40 dna synthesized after cycloheximide treatment were always lower than those observed in an infected untreated control culture. this is consistent with a requirement for a protein(s) or for protein synthesis at the initiation step in sv40 dna replication. ... | 1971 | 5543424 |
| superhelix density heterogeneity of intracellular simian virus 40 deoxyribonucleic acid. | covalently closed intracellular and viral simian virus 40 (sv40) deoxyribonucleic acid (dna) were separately isolated from infected african green monkey cells (bsc-1) grown in culture. the two dna species form overlapping bands centered at different positions in a propidium di-iodide-cesium chloride (pdi-cscl) buoyant density gradient capable of separating closed dna species with different superhelix densities. when the dense side of a (32)p-labeled intracellular dna band was mixed with the ligh ... | 1971 | 5543431 |
| a zoonosis associated with african green monkeys. | | 1968 | 5692925 |
| changes in blood glucose levels in african green monkeys following administration of sodium pentobarbital. | | 1968 | 5718755 |
| characterization of the tacaribe group of arboviruses. i. propagation and plaque assay of tacaribe virus in a line of african green monkey kidney cells (vero). | | 1967 | 6027511 |
| characterization of african green monkey b-cell lines releasing an adult t-cell leukemia-virus-related agent. | eight lymphoblastoid cell lines were established from the peripheral blood of individual african green monkeys (agm). the agm-2206 line grew out spontaneously. the others - agm-6, 7, 8, 10, 12, 13, and 16 - were obtained after infection of peripheral agm lymphocytes with cell-free culture supernatant of agm-2206. all lines contained, and were probably transformed by, agm-ebv. moreover, they expressed immunoglobulins but lacked the leu l t-cell marker. thus they were b cells. since a high percent ... | 1984 | 6086534 |
| primary isolation and serial passage of hepatitis a virus strains in primate cell cultures. | although several primate cell types have been reported to support replication of hepatitis a virus, optimal conditions for the isolation and production of quantities of virus have not been defined. we therefore examined seven different primate cell types for their ability to support replication of primate-passaged and wild-type virus as reflected by intracytoplasmic accumulation of viral antigen (direct immunofluorescence and radioimmunoassay) and propagation of cell culture-adapted virus. of th ... | 1984 | 6086708 |
| rapid differentiation of herpes simplex virus types 1 and 2 by cytopathic effect in bs-c-1 cells. | conditions are defined under which herpes simplex virus (hsv) type 2 produces a characteristic cytopathic effect (c.p.e.) in african green monkey kidney (bs-c-1) cells. the bs-c-1 microplate c.p.e. test was simultaneously evaluated against immunological and biological marker procedures on 359 hsv strains. a bs-c-1 cell suspension was added to serial dilutions of hsv strains in falcon micro test ii plates and incubated at 36.5 degrees c in a 4% co2 atmosphere. when the infected cultures were exam ... | 1984 | 6086814 |
| interruption of a human nuclear sequence homologous to mitochondrial dna by a member of the kpni 1.8 kb family. | we reported that several dna sequences homologous to mitochondrial dna (mtdna) are present in the human nuclear genome (tsuzuki et al. (1983) gene 25, 223-229). detailed southern blot analyses revealed that one of such sequences is interrupted by a repetitive sequence about 1.8 kb long, and that the insert is one member of the dispersed repeated dna sequences of the kpni 1.8 kb family. nucleotide sequence analysis showed that the kpni 1.8 kb dna is flanked with imperfect 15-base pair (bp) direct ... | 1984 | 6087277 |
| transmission and scanning electron microscopy of experimental pulmonary simian varicella (delta herpesvirus) infection in african green monkeys (cercopithecus aethiops). | the lungs of african green monkeys infected with simian varicella virus (delta herpesvirus) were examined by scanning and transmission electron microscopy. endothelial cell shrinkage, oedema formation and necrosis characterized the scanning electron microscopy of the affected vasculature. oedema of the interalveolar septa with swelling of the capillary endothelial cells was observed by transmission electron microscopy. numerous viral particles were associated with a generalized area of necrosis ... | 1984 | 6088596 |
| nucleosome arrangement in alpha-satellite chromatin of african green monkey cells. | by analyzing the accessibility of restriction endonuclease sites in african green monkey alpha-satellite chromatin, we demonstrate the absence of a unique phase relationship between nucleosomes and alpha-satellite dna. the data indicate a minimum of three different positions for nucleosome cores relative to the alpha-satellite sequence and suggest a random distribution in at least some regions. in addition, while we confirm published reports that staphylococcal nuclease cuts the alpha-satellite ... | 1984 | 6089117 |
| analysis of two potential shuttle vectors containing herpes simplex virus defective dna. | two potential shuttle vectors which contained the identical herpes simplex virus type 1 (hsv-1) defective particle dna (ddna), but prokaryotic dna of different origins, were examined for their stability when propagated in eukaryotic cells, and for their efficiency as shuttle vectors. each chimeric molecule contained a 9.5 kilobase-pair (kb) ecori fragment (hsv12-7) representing a single unit of a class i hsv-1 ddna. this ddna was cloned into the bacteriophage lambda (lambda) vector lambda gtwes ... | 1984 | 6090565 |
| continuous reorganization leads to extensive polymorphism in a monkey centromeric satellite. | previously we reported the existence of a highly polymorphic satellite, deca-satellite, in the african green monkey genome; deca-satellite probe anneals to complex sets of repeated restriction endonuclease fragments that differ from individual to individual in the monkey population. here we present experiments aimed at clarifying the structure and organization of deca-satellite sequences and investigating the mechanisms that generate the polymorphisms. deca-satellite represents less than 1% of t ... | 1984 | 6094831 |
| conservation of the human col1a1-tk-gaa synteny and homoeologous assignment in the african green monkey and the baboon (cercopithecoidae). | the pro alpha 1 (i) collagen structural gene (col1a1), the acid alpha-glucosidase (gaa), and the thymidine kinase (tk) genes, known to be closely linked in man (hsa) and mapped to hsa17, were found syntenic in two cercopithecoidae species, the baboon (papio papio, ppa) and the african green monkey (cercopithecus aethiops, cae) and assigned to homoeologous chromosomes, ppa16 and cae19, respectively. the assignment of col1a1 was obtained using two human cdna probes. hind iii restriction sites foun ... | 1984 | 6096258 |
| investigation of antiviral activity of 1-beta-d-arabinofuranosylthymine (ara-t) and 1-beta-d-arabinofuranosyl-e-5-(2-bromovinyl)uracil (bv-ara-u) in monkeys infected with simian varicella virus. | 1-beta-d-arabinofuranosylthymine (ara-t) and 1-beta-d-arabinofuranosyl-e-5-(2-bromovinyl)uracil (bv-ara-u) were shown to have antiviral activity in vitro and in vivo against simian varicella virus. both compounds successfully prevented clinical disease caused by inoculation of african green monkeys with simian varicella virus, eliminating the development of rash and substantially suppressing viremia. ara-t treatment was effective by either intraperitoneal or oral routes of administration and bv- ... | 1984 | 6097178 |
| a transformed vero cell line stably producing the hepatitis b virus surface antigen. | we have constructed plasmids in which transcription of the surface antigen gene of the human hepatitis b virus (hbsag) is under the control of the sv40 early promoter. these plasmids have been used to analyze the expression of the hbsag gene, both in mouse cells and in african green monkey kidney (vero) cells. we have established a vero cell line continuously expressing hbsag that is indistinguishable from authentic 22 nm hbsag particles. post-transcriptional modification of hbsag mrna also appe ... | 1984 | 6098537 |
| characterization of simian retrovirus genome related to human t-cell leukemia virus type i. | human t-cell leukemia virus type i (htlv-i) is exogenous for human transmission by viral infection and was shown to be a causative agent of adult t-cell leukemia (atl) in man. monkeys of several species were found to have antibodies reactive with htlv-i antigens and thus infection with htlv-i like retroviruses was suspected. the retroviruses in several species of monkeys were characterized by southern hybridization, molecular cloning and sequencing. these monkey retroviruses, tentatively called ... | 1984 | 6100642 |
| identification of the metabolites of di-(2-ethylhexyl) phthalate in urine from the african green monkey. | the metabolites of di-(2-ethylhexyl) phthalate (dehp) found in urine from african green monkeys after intravenous administration of the 14c-labeled parent compound were isolated and identified. criteria of identification included cochromatography with rat-derived standards on direct-phase hplc and a variety of gas-chromatographic columns, as well as correspondence of mass spectra (70-ev electron impact and methane positive chemical ionization) with those of known standards. approximately 80% of ... | 1981 | 6113930 |
| spinal nerve distributions in the upper limb: the organization of the dermatome and afferent myotome. | single fibres were dissected from the dorsal spinal roots of the nerves serving the brachial plexus in african green monkeys. the dermatomal organization of these spinal nerves was deduced from data concerning the receptive fields of 2834 single afferent fibres. these data were collected in an attempt to reconcile some of the discrepancies that exist in published descriptions of the dermatomes in primates; our results and the literature reviewed suggest that the cutaneous region served by one sp ... | 1981 | 6115429 |
| laboratory vector studies on six mosquito and one tick species with chikungunya virus. | the tick ornithodoros savignyi and the mosquitoes culex horridus, culex quinquefasciatus, aedes fulgens, ae. furcifer and mansonia africana were tested for infection rates and ability to transmit chikungunya virus. o. savignyi and cx quinquefasciatus did not become infected and cx quinquefasciatus failed to transmit the virus between vervet monkeys, cercopithecus aethiops. only one of 17 cx horridus feeding on a blood-virus mixture became infected which included infection of the salivary glands. ... | 1981 | 6115488 |
| formation of "ghost" bodies and calcification in experimental atherosclerosis in nonhuman primates. an ultrastructural study. | aortic lesions from african green monkeys fed a cholesterol diet for up to 24 months were studied by electron microscopy. the lesions, grossly classified as fatty dots and fatty streaks consisted of foam cells, increased amounts of interstitial connective tissues and osmiophilic lipid material. in addition, in the interstitial spaces, there were membrane-bound detached cytoplasmic fragments and deeply osmiophilic calcium spherules. the smooth muscle cells had a frayed appearance and bulbous cyto ... | 1981 | 6119840 |
| inhibition of cellular and virus-associated nucleotide polymerases by, and anti-herpes simplex virus activity of, streptovaricin derivatives. | fourteen streptovaricin derivatives were tested for inhibition of cellular nucleotide polymerases (deoxyribonucleic acid polymerases alpha, beta, and gamma, terminal deoxynucleotidyltransferase [tdt], and ribonucleic acid polymerase ii), simian sarcoma virus deoxyribonucleic acid polymerase, and herpes simplex virus type 1-induced deoxyribonucleic acid polymerase (hsv-dp). three compounds (strep-tovadienal c, prestreptovarone, and streptoval fc) preferentially inhibited tdt and hsv-dp over the o ... | 1981 | 6119956 |
| african green monkeys are infected with adult t-cell leukaemia virus or closely related agent. | | 1983 | 6130270 |
| antiherpes activity of some novel analogues of (e)-5-(2-bromovinyl)-2'-deoxyuridine (e-brvudr) in two different cell lines. | in a series of 5-vinyl-2'-deoxyuridine (vudr) analogues (5-(2-x-vinyl)-udrs) the (e)-5-(2-bromovinyl)-udr (e-brvudr) proved the most potent inhibitor of plaque formation of two herpes simplex virus type 1 (hsv-1) strains in human embryonic lung fibroblast (helf) and african green monkey kidney (vero) cell cultures. the (z)-5-(2-bromovinyl)-udr (z-brvudr) isomer and the 5-(2,2-dibromovinyl)-udr (br2vudr) analogue were 10-20 times less efficient, whereas the (e)-5-(2-cyanovinyl)-udr (cnvudr) and t ... | 1984 | 6143491 |
| effect of immunosuppression on morphological changes in cns of monkeys infected with different measles virus vaccine strains. | residual neurovirulence of measles virus vaccine strains "leningrad-16", "moscow-5" and of cloned variant of l-16 strain--clone 3, differing in the degree of attenuation, was studied in intracerebrally (i.c.) infected cercopithecus aethiops monkeys, receiving cortisone and/or antilymphocyte serum (als). whereas the sensitivity for detection of the potential neurovirulence of vaccine strains was not increased by cortisone treatment. als had such effect in detection of neurotropic properties of th ... | 1984 | 6145348 |
| prostaglandin a compounds as antiviral agents. | prostaglandins of the a series strongly inhibit the production of sendai virus in african green monkey kidney cells and are able to prevent the establishment of persistent infection ("carrier" state). this action is specific for prostaglandin a and is not due to alteration in the host cell metabolism or in the virus infectivity. the possibility that this effect is mediated by interferon is discussed. | 1980 | 6157190 |
| [chromosome nucleolus organizer regions of the african green monkey]. | the silver staining ag-i for detection of nucleolar organizer regions (nor) in the african green monkey chromosomes was used. nor were situated on the secondary constrictions of one pair of homologous marker chromosomes and consisted of two parts. a block of condensed chromatin was seen in the middle of the despiralized portion corresponding to the secondary constriction. the data suggest that the stalks of satellites of chromosomes, rather than satellites themselves, are regions of nucleolar or ... | 1980 | 6157237 |
| characterization of membrane permeability alterations induced in vero cells by clostridium perfringens enterotoxin. | alterations in plasma membrane permeability induced by clostridium perfringens enterotoxin were studied using vero (african green monkey kidney) cells which were radioactively labeled with four markers of different molecular size. the markers were alpha-amino[14c]isobutyric acid (mr 103), 3h-labeled nucleotide (mr approx. 300), 51cr label (mr approx. 3000) and [3h]rna (mr>25000). over a 2h period, enterotoxin caused significant release of aminoisobutyric acid, nucleotides and 51cr label but not ... | 1980 | 6157413 |
| ineffectiveness of adenine arabinoside and adenine arabinoside 5'-monophosphate in simian varicella infection. | adenine arabinoside and adenine arabinoside 5'-monophosphate (ara-amp) have been evaluated for antiviral activity against simian varicella virus infection in monkeys. in a preliminary study for toxicity, intramuscular injection of ara-amp at 15 mg/kg per day as a single injection for 5 days to two normal patas monkeys caused no detectable local reaction, no weight loss or changes in serum transaminase levels, and no hematological abnormalities. when this dose was given in the treatment of four s ... | 1980 | 6158291 |
| complex of simian virus 40 large-t antigen and host 53,000-molecular-weight protein in monkey cells. | mouse cells transformed by simian virus 40 (sv40) have been shown to contain a complex of the virus-coded large-t antigen with a host 53,000-molecular-weight (53k) protein. initial attempts to detect a similar complex in lytically infected cells were unsuccessful, and it therefore seemed that the complex might be peculiar to transformed or abortively transformed nonpermissive cells. immunoprecipitation of [32p]phosphate-labeled extracts of sv40-infected cv-1 african green monkey kidney cells wit ... | 1981 | 6163871 |
| aortic sudanophilia an zinc-copper ratios in the liver of vervet monkeys fed different types of dietary fibre. | vervet monkeys (cercopithecus aethiops pygerethrus) were fed a semipurified diet containing 40% sucrose, 25% casein, 14% hydrogenated coconut oil and 15% fibre for 6 months. alfalfa, cellulose, or wheat straw was the source of the fibre. all the diets led to aortic sudanophilia, but the group fed wheat straw had the highest copper level in the liver and the least sudanophilia. the order to severity of the sudanophilia, the ratio of zinc to copper in the fibers, and the ratio of zinc to copper in ... | 1981 | 6164104 |
| identification of antigenically related polypeptides at centrioles and basal bodies. | an antigen localized at the centriolar region has been identified by indirect immunofluorescence studies in african green monkey kidney, human, hamster, rat, and mouse cells. the antigen consists of two polypeptides of 14,000 and 17,000 daltons. a related antigen is also present at the basal body region in ciliated cells from chicken, cat, mouse, pig, steer, and rabbit trachea and from rabbit fimbria. immunoelectron microscopy shows that the immunoreactive antigen is indeed located in the region ... | 1981 | 6166008 |
| prostaglandins of the a series inhibit sendai virus replication in cultured cells. | prostaglandins of the a series (pga) were shown to be potent inhibitors of sendai virus replication in african green monkey kidney cells in culture. this antiviral activity was specific for pga. the effective dose (4 micrograms/ml) was not toxic to the cells and did not alter either host cell metabolism of infectivity of the virus. pga did not affect the first stages of virus replication and seemed to act by inhibiting virus maturation and/or budding from the cells. the antiviral action of pga w ... | 1981 | 6168737 |
| construction and expression in vivo of an internally deleted mouse alpha-fetoprotein gene: presence of a transcribed alu-like repeat within the first intervening sequence. | an alpha-fetoprotein (afp) 'minigene' was constructed by joining the first two exons along with 0.9 kilobases of 5' flanking sequence of the mouse afp gene to its last exon and 0.4 kilobases of 3' flanking sequence. this 'minigene' was tested for activity by inserting it into an sv40 vector and infecting african green monkey kidney cells. correct initiation, termination, polyadenylation and splicing were observed. furthermore a 220 nucleotide transcript was detected and mapped to a mouse alu-lik ... | 1982 | 6179043 |
| stimulation of host centriolar antigen in tc7 cells by simian virus 40: requirement for rna and protein syntheses and an intact simian virus 40 small-t gene function. | simian virus 40 (sv 40) stimulated a host cell antigen in the centriolar region after infection of african green monkey kidney (agmk) cells. the addition of puromycin and actinomycin d to cells infected with sv40 within 5 h after infection inhibited the stimulation of the host cell antigen, indicating that de novo protein and rna syntheses that occurred within the first 5 h after infection were essential for the stimulation. early viable deletion mutants of sv40 with deletions mapping between 0. ... | 1982 | 6180184 |
| 8-azaguanine resistant african green monkey kidney cell mutants (vero 153): isolation and characterization. | a clone of vero cells resistant to up to 20 micrograms/ml 8-azaguanine was isolated. this clone (designated vero 153) has a doubling rate of approximately 24 h and a maximum cell density of 10,000/mm2. deficiency of the enzyme hypoxanthine guanine phosphoribosyl transferase (hgprt) in vero 153 was demonstrated by methods of radiochromatography. vero 153 is susceptible to hypoxanthine-thymidine-aminopterin (hat) medium and its resistance to 8-azaguanine seems to be nonreversible. like parental ce ... | 1983 | 6190732 |
| the relationship between prostaglandins and virus replication: endogenous prostaglandin synthesis during infection and the effect of exogenous pga on virus production in different cell lines and in persistently infected cells. | african green monkey kidney cells were shown to normally synthesize immunoreactive pge1. infection of these cells with sendai virus did not alter rates of pge1 synthesis, while it stimulated interferon production. pgas, that we have previously shown to be potent inhibitors of sendai virus replication in this system, at the same dose (4 micrograms/ml), also strongly inhibited the replication of this virus in hep-2 cells and in vero cells, a monkey kidney cell line that does not produce interferon ... | 1983 | 6191363 |
| characterization of a foamy virus isolated from cercopithecus aethiops lymphoblastoid cells. | a virus derived from cells of a lymphoblastoid line originating from the lymph node of a healthy african green monkey was characterized as a typical member of the foamy virus subgroup of retroviridae by its morphological, physicochemical, biological and biochemical properties (reverse transcriptase activity). besides the usual host range of foamy viruses, the isolated strain revealed a remarkable t-lymphotropism, distinguishing it from the prototypes of foamy viruses previously isolated from afr ... | 1983 | 6193403 |
| optimal conditions for titration of sv40 by the plaque assay method. | the parameters of the simian virus 40 (sv40) plaque assay on african green monkey kidney cells were optimized for reproducibility and maximum plaquing efficiency. plaques were visible as early as 8 days postinfection; maximum titers were obtained with a 10- to 11-day incubation period. titers read 12-16 days postinfection were not significantly higher than those observed after 10-11 days. adsorption volumes greater than 0.1 ml/60 mm petri dish decreased plaque forming units (pfus) detected. time ... | 1983 | 6195175 |
| monoclonal antibodies against the complement control protein factor h (beta 1 h). | two mouse monoclonal antibodies against the human complement control protein, factor h (beta 1h), are described. the antibodies are both igg - gamma 1 - subclass and are directed against different epitopes on the human factor h molecule. one of the antibodies, mrc ox 24, increases the cofactor activity of factor h in factor i-mediated cleavage of soluble c3b. the second antibody, mrc ox 23, which has no effect alone, reduces the increase in cofactor activity observed in the presence of the first ... | 1983 | 6199050 |
| interferon response to poly ic/poly-l-lysine in normal and lymphocyte-suppressed primates. | grivet monkeys immunosuppressed with either cyclophosphamide or methylprednisolone retain their capacity to produce interferon in response to poly i:c/poly-l-lysine (poly icl). to stimulate a human immunosuppressed condition, monkeys were maintained in a lymphocyte suppressed state (500-2200 lymphocytes/mm3, mean = 1550) for five weeks by weekly intravenous injections of cyclophosphamide (50 mg/kg i.v.). monkeys similarly treated with phosphate buffered saline retained lymphocytes within the nor ... | 1984 | 6201567 |
| a tumor promoter induces rapid and coordinated reorganization of actin and vinculin in cultured cells. | treatment of epithelial african green monkey kidney (bsc-1) cells with the potent tumor promoter 12-o-tetradecanoylphorbol-13-acetate (tpa) induces a rapid and reversible redistribution of actin and vinculin that is detectable after only 2 min of treatment. within 20-40 min, stress fibers disappear, while at the same time large actin-containing ribbons resembling ruffles develop both at the cell periphery and in more central regions. vinculin is associated with these actin ribbons or bands in a ... | 1984 | 6206076 |
| interaction of small nuclear ribonucleoproteins with simian virus 40 in cv-1 cells: is u2 snrna involved in regulating replication? | the small nuclear rnas (snrnas) in african green monkey kidney cells (cv-1 cells) were examined by polyacrylamide gel electrophoresis. methodology was developed to improve their extraction from enriched fractions. cellular fractionation studies and subsequent analysis of these rnas indicate that they are tightly associated with chromatin. treatment of cells with alpha-amanitin totally suppressed transcription of u1, u2, u4, u5, and partially suppressed transcription of u6, suggesting that these ... | 1984 | 6210183 |
| organization of african green monkey dna at junctions between alpha-satellite and other dna sequences. | | 1982 | 6213785 |
| functional analysis of monkey lymphocyte subsets defined by okt4 and okt8 monoclonal antibodies. | the percentages of rhesus monkey blood lymphocytes (pbl) reactive with okt4 and okt8 antibodies and the okt4/okt8 ratio showed significant correlations with the log of the immunoglobulin plaque-forming cell (pfc) response after stimulation with pokeweed mitogen (pwm). these correlations suggested that monkey okt4+ cells function as "helper" cells and okt8+ cells function as "suppressor" cells for the pfc response. this was confirmed by separation and study of enriched t- and b-cell subpopulation ... | 1983 | 6221805 |
| human rotavirus type 2: cultivation in vitro. | a strain of type 2 human rotavirus (wa) was grown to relatively high titer through 14 passages in primary cultures of african green monkey kidney (agmk) cells. this passage series was initiated with virus that had been passaged 11 times serially in newborn gnotobiotic piglets. in contrast, virus present in the stool of patient wa as well as virus from the first, second, or third passage in piglets could not be propagated successfully in african green monkey kidney cells. prior to each passage in ... | 1980 | 6243190 |
| characterization of an ebv-like virus from african green monkey lymphoblasts. | | 1980 | 6243826 |