| fluorescence correlation spectroscopy investigation of a gfp mutant-enhanced cyan fluorescent protein and its tubulin fusion in living cells with two-photon excitation. | this study investigates the feasibility of using the enhanced cyan mutant of green fluorescent protein (ecfp) as a probe for two-photon fluorescence correlation spectroscopy (fcs). molecular dynamics and other properties of ecfp and an ecfp-tubulin fusion protein were investigated in living potorous tridactylis (ptk2) cells. ecfp has high molecular brightness in the nucleus (eta=3.3 kcpsm) and in the cytoplasm (3.2 kcpsm) under our experimental conditions. the diffusion constants of ecfp were de ... | 2007 | 15065907 |
| calmodulin bound to stress fibers but not microtubules involves regulation of cell morphology and motility. | calmodulin (cam) is a major cytoplasmic calcium receptor that performs multiple functions including cell motility. to investigate the mechanism of the regulation of cam on cell morphology and motility, first we checked the distribution of cam in the living cells using gfp-cam as an indicator. we found that gfp-cam showed a fiber-like distribution pattern in the cytosol of living potorous tridactylis kidney (ptk2) cells but not in living hela cells. the endogenous cam in heavily permeabilized hel ... | 2008 | 17884685 |
| an anomalous effect of hydroxyurea and visible light on nucleic acids of a photoreactivating mammalian cell potorous tridactylis. | | 1972 | 4536933 |
| effects of the pyrrolizidine alkaloid heliotrine on cell division and chromosome breakage in cultures of leucocytes from the marsupial potorous tridactylus. | | 1968 | 5664136 |
| chromosomes in a marsupial (potorous tridactylis) tissue culture. | | 1962 | 14004541 |
| investigation of the mechanism of retraction of the cell margin and rearward flow of nodules during mitotic cell rounding. | we have studied two types of cell motility directed toward the cell center: retraction of the cell margin and rearward flow of small cytoplasmic nodules during mitotic cell rounding in potoroo tridactylis kidney (ptk2) cells by time-lapse video microscopy, drug treatments, and photoactivation of fluorescence. nodules flow rearward on thin, actin-rich fibers (retraction fibers) exposed as the cell margin retracts. retraction of the cell margin and rearward flow of nodules require intact actin fil ... | 1997 | 9017599 |
| adaptive changes in trabecular architecture in relation to functional strain patterns and disuse. | principal strains and their orientation, determined from in vivo and in situ strains recorded from the lateral cortical surface of the calcaneus of potoroos (a small marsupial) during treadmill exercise and tension applied via the achilles tendon, were compared with the underlying trabecular architecture and its alignment to test wolff's "trajectorial theory" of trabecular alignment. in vivo and in situ principal compressive strains (-800 to -2000 mu e) were found to be aligned (mean 161 +/- 7 d ... | 1996 | 8830980 |
| energetic cost of locomotion as a function of ambient temperature and during growth in the marsupial potorous tridactylus. | in the marsupial, the potoroo, multiple regression analysis shows that ambient temperature makes a minor (2%) contribution towards variation in oxygen consumption with speed. this suggests that the heat generated during running is substituted for heat which would otherwise have to be generated for temperature regulation. maximum levels of oxygen consumption are also temperature-independent over the range 5-25 degrees c, but plasma lactate concentrations at the conclusion of exercise significantl ... | 1993 | 8440970 |
| photoreactivation of ultraviolet radiation-induced release of arachidonic acid from marsupial cells. | exposure of an established marsupial cell line, ptk2 (potorous tridactylus), to ultraviolet radiation (uvr) from an fs-40 sunlamp (280-400 nm) resulted in a fluence-dependent release of radiolabeled arachidonic acid (aa) from cell membranes. post-uvr, but not pre-uvr, exposure to photoreactivating light reversed uvr-induced pyrimidine dimers in dna and suppressed the uvr-induced release of aa. these data indicate that dna damage contributes to the release of aa from membrane phospholipids. | 1991 | 1665911 |
| in vitro photosensitization ii. an electron microscopy study of cellular destruction with mono-l-aspartyl chlorin e6 and photofrin ii. | primary sites of subcellular destruction with photofrin ii (pfii) and mono-l-aspartyl chlorin e6 (mace) were determined by transmission electron microscopy (tem). potorous tridactylus (ptk2) cells were grown in rose chambers and incubated for 24 hr with a sensitizer concentration sufficient to provide 100% mortality. cells were irradiated with laser light and fixed and processed for electron microscopy at various times post-irradiation. the results indicate that pfii initially destroys mitochond ... | 1989 | 2523992 |
| growth and role of the corpus luteum throughout delayed gestation in the potoroo, potorous tridactylus. | the growth cycle of the corpus luteum (cl) of the potoroo is similar to that of other macropodids. during delayed gestation, the post-partum cl remains quiescent until it is reactivated by removal of the sucking pouch young. the cl then undergoes a period of growth, rapid from day 6 until day 12, followed by a gradual decline from day 21 to day 27. excision of the cl before day 6 of pregnancy either inhibited embryonic development or failed to support it. excision of the cl between 6 and 21 days ... | 1986 | 3944807 |
| effect of cadmium on the reproductive organs of the male potoroo potorous tridactylus (macropodidae). | low (2.28 mg/kg) or high (4.56 mg/kg) doses of cadmium chloride were administered, either intraperitoneally or subcutaneously, to adult male potoroos (p. tridactylus). after 7 days, the testes, caput epididymis and spermatic cords of the high-dose groups all displayed a degree of cellular damage; however, the damage was not as extensive as that which occurs in some eutherian species after only 24 h. this time differential might be attributed to structural differences between the testicular blood ... | 1985 | 4091756 |
| cycloheximide-sensitive recovery from 254 nm uv light damage in cultured marsupial cells. | colony-forming ability of potorous tridactylus-kidney (ptk-2) cells was measured after exposure to 254-nm ultraviolet (uv) light and cycloheximide. addition of 5 microm cycloheximide after exposure of the cells to uv light greatly decreased cell survival. maximum effect of the drug was obtained by 24-h exposure after irradiation. the cycloheximide sensitivity of irradiated cells was eliminated if addition of the drug was delayed for 8-10 h after irradiation, or if the cells received photoreactiv ... | 1984 | 6472330 |
| further observations on the potoroo (potorous tridactylus) in captivity. | the maintenance and breeding performance of potoroos in captivity over a 7-year period is described. by removing offspring from the pouch and allowing 29 days for completion of embryonic development and birth to occur, pouch young of known ages can be obtained. | 1983 | 6865321 |
| establishment of nucleolar deficient sublines of ptk2 (potorous tridactylis) by ultraviolet laser microirradiation. | one of the two nucleoli of tetraploid ptk2 wa cells in early prophase was irradiated with an ultraviolet (uv) laser microbeam. the daughter cells that maintained the nucleolar deficiency were isolated and cloned. five nucleolar deficient sublines of ptk2 wa were established, thus providing an experimental system to study the ribosomal gene-nucleolar organizer complex. | 1983 | 6840209 |
| the effects of hypoxia and co2 on the sleep-waking pattern of the potoroo (potorous tridactylus apicalis). | four male potoroos (potorous tridactylus apicalis) breathed 21% and 7% o2 with and without the addition of 5% co2. the effects of these gas mixtures on the potoroo's sleeping-waking pattern (swp) were studied. the swp while breathing 21% o2/5% co2 was unchanged when compared with that of breathing ambient air (21% o2). while breathing 7% o2, the swp was severely disrupted: total sleep time (tst) and slow wave sleep (sws) increased markedly. brain temperature fell substantially. paradoxical sleep ... | 1983 | 6405411 |
| hyperoxaluria, hyperglycoluria and renal oxalosis in gilbert's potoroos (potorous gilbertii). | six gilbert's potoroos (potorous gilbertii) in a captive colony, five of which were closely related, died or were euthanased with severe renal disease. clinical signs were mostly non-specific. renal calculi were seen on ultrasound of two affected potoroos and oxalate crystalluria was seen in two of three affected potoroos that had urine samples examined. necropsies revealed extensive severe renal oxalosis in all affected potoroos. these findings and markedly increased concentrations of glycolate ... | 2017 | 28653383 |
| monitoring reproduction in the critically endangered marsupial, gilbert's potoroo (potorous gilbertii): preliminary analysis of faecal oestradiol-17beta, cortisol and progestagens. | gilbert's potoroo (potorous gilbertii) was rediscovered in 1994 after having been presumed extinct for 120 years. estimates indicate fewer than 40 individuals remain at two peoples bay nature reserve on the south coast of western australia although a translocated population of approximately 20 animals has recently been established on nearby bald island. a captive breeding facility has been established adjacent to the mainland population but few young have been produced (8 since 1995). faecal lev ... | 2010 | 19539621 |
| characterization of microsatellite loci in the endangered long-footed potoroo potorous longipes. | | 1997 | 9161018 |
| numa recruits dynein activity to microtubule minus-ends at mitosis. | to build the spindle at mitosis, motors exert spatially regulated forces on microtubules. we know that dynein pulls on mammalian spindle microtubule minus-ends, and this localized activity at ends is predicted to allow dynein to cluster microtubules into poles. how dynein becomes enriched at minus-ends is not known. here, we use quantitative imaging and laser ablation to show that numa targets dynactin to minus-ends, localizing dynein activity there. numa is recruited to new minus-ends independe ... | 2017 | 29185983 |
| draft de novo transcriptome of the rat kangaroo potorous tridactylus as a tool for cell biology. | the rat kangaroo (long-nosed potoroo, potorous tridactylus) is a marsupial native to australia. cultured rat kangaroo kidney epithelial cells (ptk) are commonly used to study cell biological processes. these mammalian cells are large, adherent, and flat, and contain large and few chromosomes-and are thus ideal for imaging intra-cellular dynamics such as those of mitosis. despite this, neither the rat kangaroo genome nor transcriptome have been sequenced, creating a challenge for probing the mole ... | 2015 | 26252667 |
| cellular localisation of antitumoral 6-alkyl thymoquinones revealed by an alkyne-azide click reaction and the streptavidin-biotin system. | the subcellular distribution and accumulation of thymoquinone 1, a natural anticancer agent, has hitherto been unknown. we prepared 6-(dec-9-ynyl)thymoquinone 3, an alkyne-labelled derivative with anticancer activity similar to that of its parent compound 1. alkyne 3 was seen, after a huisgen-type click reaction with 3-azido-7-hydroxycoumarin, to accumulate in distinct compartments of the nuclei of ptk(2) potoroo kidney cells, and in adjoining regions that were stained with an antibody specific ... | 2011 | 21500333 |
| chromosome tips damaged in anaphase inhibit cytokinesis. | genome maintenance is ensured by a variety of biochemical sensors and pathways that repair accumulated damage. during mitosis, the mechanisms that sense and resolve dna damage remain elusive. studies have demonstrated that damage accumulated on lagging chromosomes can activate the spindle assembly checkpoint. however, there is little known regarding damage to dna after anaphase onset. in this study, we demonstrate that laser-induced damage to chromosome tips (presumptive telomeres) in anaphase o ... | 2010 | 20811641 |
| an analysis of chromosomal sensitivity of hpk1 cells from the marsupial potorous tridactylus to x-rays and to heliotrine. | | 2003 | 5065177 |
| the origin, course and termination of the corticospinal tracts of the tasmanian potoroo (potorous apicalis). | | 1972 | 5039183 |
| skin structure in newborn marsupials with focus on cutaneous gas exchange. | a morphological and morphometric study of the skin of a variety of newborn marsupials (dasyurus viverrinus, monodelphis domestica, trichosurus vulpecula, isoodon obesulus, perameles nasuta, phascolarctos cinereus, potorous tridactylus, petrogale penicillata, thylogale thetidi, macropus dorsalis) and of a monotreme hatchling (ornithorhynchus anatinus) was undertaken to assess the possibility of cutaneous gas exchange. additionally, the lungs of some of these species were investigated to assess it ... | 2018 | 29947022 |
| sequence analyses at mitochondrial and nuclear loci reveal a novel theileria sp. and aid in the phylogenetic resolution of piroplasms from australian marsupials and ticks. | the order piroplasmida encompasses two main families: babesiidae and theileriidae, containing tick-borne pathogens of veterinary and medical importance worldwide. while only three genera (babesia, cytauxzoon and theileria) comprising piroplasm parasites are currently recognised, phylogenetic studies at the 18s rrna (18s) gene suggest that these organisms represent at least ten lineages, one of which comprises the relatively unique and highly diverse theileria spp. from australian marsupials and ... | 2019 | 31851687 |
| morphological observations on the diapausing blastocyst of some macropodid marsupials. | diapausing blastocysts from the uteri of females of 11 macropodid species were fixed, torn, flattened and stained for examination of the protoderm. the protoderm remained undifferentiated and unilaminar throughout diapause. the number of cells in the protoderm did not vary with age in macropus rufus or m. eugenii. protoderm cell number decreased slightly with age in m. rufogriseus banksianus and appeared to decrease in potorous tridactylus. the diameter of the shell did not change appreciably wi ... | 1981 | 7205794 |
| a biometric analysis of the chromosomes of the marsupials--macropus major, macropus rufus and potorous tridactylis. | | 1965 | 14332557 |
| transfection of pseudouridine-modified mrna encoding cpd-photolyase leads to repair of dna damage in human keratinocytes: a new approach with future therapeutic potential. | uvb irradiation induces harmful photochemical reactions, including formation of cyclobutane pyrimidine dimers (cpds) in dna. accumulation of unrepaired cpd lesions causes inflammation, premature ageing and skin cancer. photolyases are dna repair enzymes that can rapidly restore dna integrity in a light-dependent process called photoreactivation, but these enzymes are absent in humans. here, we present a novel mrna-based gene therapy method that directs synthesis of a marsupial, potorous tridacty ... | 2013 | 24211294 |
| a novel approach to assessing the ecosystem-wide impacts of reintroductions. | reintroducing a species to an ecosystem can have significant impacts on the recipient ecological community. although reintroductions can have striking and positive outcomes, they also carry risks; many well-intentioned conservation actions have had surprising and unsatisfactory outcomes. a range of network-based mathematical methods has been developed to make quantitative predictions of how communities will respond to management interventions. these methods are based on the limited knowledge of ... | 2019 | 30312496 |
| elucidating patterns in the occurrence of threatened ground-dwelling marsupials using camera-traps. | establishing trends in endangered fauna against management efforts is a key but often challenging enterprise. camera-traps offer a new and literal window into monitoring many different mammalian species. getting it right demands seeking baseline information about how often target species interact with these devices, prior to setting a long-term monitoring strategy. we used a camera-trap array to collect detection data on three species of threatened ground-dwelling marsupials in south-eastern mai ... | 2019 | 31684179 |
| identification of a novel species of eimeria schneider, 1875 from the woylie, bettongia penicillata gray (diprotodontia: potoroidae) and the genetic characterisation of three eimeria spp. from other potoroid marsupials. | faecal samples (n = 1,093) collected from the woylie bettongia penicillata gray, in south-western australia were examined for the presence of coccidian parasites. eimeria sp. oöcysts were detected in 15.2% of samples. faecal samples obtained from the eastern bettong bettongia gaimardi (desmarest) (n = 4) and long-nosed potoroo potorous tridactylus (kerr) (n = 12) in tasmania, were also screened for the presence of eimeria spp. (prevalence 50% and 41.7%, respectively). morphological and genetic c ... | 2019 | 31332672 |
| habitat and introduced predators influence the occupancy of small threatened macropods in subtropical australia. | australia has had the highest rate of mammal extinctions in the past two centuries when compared to other continents. frequently cited threats include habitat loss and fragmentation, changed fire regimes and the impact of introduced predators, namely the red fox (vulpes vulpes) and the feral cat (felis catus). recent studies suggest that australia's top predator, the dingo (canis dingo), may have a suppressive effect on fox populations but not on cat populations. the landscape of fear hypothesis ... | 2019 | 31236222 |
| chromatin influence on the function and formation of the nuclear envelope shown by laser-induced psoralen photoreaction. | potorous tridactylis (ptk2) cells growing in culture were treated with psoralen derivatives and dividing cells were located by phase-contrast microscopy. psoralens, light-sensitive dna-photoadducting drugs, were reacted with mitotic chromosomes through exposure to 365-nm light from an argon laser microbeam system. it was found that following mitosis and photoreaction, cells without nuclear envelopes were produced when psoralen-treated cells received 60 light pulses over their entire chromosome c ... | 1978 | 701393 |
| oxygen consumption and nitrogen metabolism in the potoroo, potorous tridactylus. | | 1976 | 9227 |
| locomotion in the potoroo. | | 1974 | 4819593 |
| the management of a small colony of the marsupial potorous tridactylus, and a record of its breeding in captivity. | | 1972 | 4673209 |