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fatal rameshwarotrema uterocrescens infection with ulcerative esophagitis and intravascular dissemination in green turtles.the trematode rameshwarotrema uterocrescens (digenea: pronocephalidae) parasitizes the glands of the caudal esophagus of chelonia mydas. in the present study, 741 c. mydas were examined, 85 animals had adult specimens of r. uterocrescens associated with necrotizing ulcerous esophagitis, of these 85, 21 presented invasion of the esophageal mural vessels in the caudal esophagus of juvenile green turtles (chelonia mydas). necrotizing granulomatous splenitis, hepatitis, and nephritis were associated ...201931289721
fathoming sea turtles: monitoring strategy evaluation to improve conservation status assessments.population monitoring must be accurate and reliable to correctly classify population status. for sea turtles, nesting beach surveys are often the only population-level surveys that are accessible. however, process and observation errors, compounded by delayed maturity, obscure the relationship between trends on the nesting beach and the population. we present a simulation-based tool, monitoring strategy evaluation (mose), to test the relationships between monitoring data and assessment accuracy, ...201931267602
development and validation of a new method for determination of pb and cr in marine organisms by total reflection x-ray fluorescence (txrf) spectroscopy.lead and chromium contamination represents one of the most serious problems in the aquatic environments. the aim of this work was to develop and validate an accurate, sensitivity, and rapid method for the simultaneous determination of pb and cr at trace levels in tissues and fat of marine organisms such as turtle (chelonia mydas), shark (rhizoprionodon terraenovae), and dolphin (tursiops truncatus), utilizing the total reflection x-ray fluorescence (txrf) spectroscopy. working solutions were pre ...201931263626
evaluation of plasma 25-hydroxyvitamin d, ionized calcium, and parathyroid hormone in green sea turtles (chelonia mydas) exposed to different intensities of ultraviolet b radiation.for many reptile species, adequate ultraviolet b (uvb) radiation is essential for proper calcium metabolism. in this study, the effects of uvb radiation on calcium metabolism were evaluated in green sea turtles (chelonia mydas). 25-hydroxyvitamin d, parathyroid hormone (pth), and ionized calcium (ica) were measured in juvenile (n = 18, 9 indoor, 9 outdoor) and adult (n = 8, 4 indoor, 4 outdoor) turtles. all animals were fed an identical diet. outdoor animals had access to unfiltered sunlight, wh ...201931260209
hg and δ15n in juvenile green turtles from southeastern brazil (~23°s): inferences about contamination levels and recruitment to coastal waters.total mercury (thg) and δ15n were analysed in liver and muscle of juvenile green turtles (chelonia mydas) from a foraging site in southeastern brazil (~23°s) to evaluate contamination levels and make inferences about the timescale that animals were recruited from oceanic to coastal waters. the values for thg and δ15n in liver and muscle were 432.2 and 72.8 μg kg-1 and 10.0‰ and 9.2‰, respectively. thg concentrations in tissues indirectly indicated that the main food resources for this consumer ( ...201931232349
migratory corridor linking atlantic green turtle, chelonia mydas, nesting site on bioko island, equatorial guinea to ghanaian foraging grounds.this study uses satellite telemetry to track post-nesting movements of endangered green turtles (chelonia mydas) (n = 6) in the gulf of guinea. it identifies a migratory corridor linking breeding grounds of atlantic green turtles nesting on bioko island, equatorial guinea, to foraging grounds in the coastal waters of accra, ghana. track lengths of 20-198 days were analyzed, for a total of 536 movement days for the six turtles. migratory pathways and foraging grounds were identified by applying a ...201931226114
nasal cavity of green sea turtles contains 3 independent sensory epithelia.the morphological and histological features of the nasal cavity are diverse among animal species, and the nasal cavities of terrestrial and semiaquatic turtles possess 2 regions lined with each different type of sensory epithelium. sea turtles can inhale both of volatile and water-soluble odorants with high sensitivity, but details of the architectural features and the distribution of the sensory epithelia within the sea turtle nasal cavity remain uncertain. the present study analyzed the nasal ...201931155674
chelonid alphaherpesvirus 5 dna in fibropapillomatosis-affected chelonia mydas.fibropapillomatosis is a panzootic and chronic disease among chelonia mydas-usually associated with anthropogenic impacts. this study contributes towards understanding fibropapillomatosis implications for c. mydas populations as a reflector of environmental quality, via prevalence and histological, molecular and blood analyses at a world heritage site in southern brazil. sixty-three juvenile c. mydas (31.3-54.5 cm curved carapace length-ccl) were sampled during two years. eighteen specimens (~ 2 ...201931124021
diversity, immunoregulatory action and structure-activity relationship of green sea turtle cathelicidins.cathelicidins are a family of gene-encoded immune effectors in vertebrate innate immunity. here, we reported the diversity and biological activity of cathelicidins in green sea turtle, a marine reptile species known for long lifespan and disease resistance. four novel cathelicidins (cm-cath1-4) were identified from green sea turtle. all of them, especially cm-cath2, exhibited potent, broad-spectrum and rapid bactericidal and anti-biofilm activities by inducing the disruption of cell membrane int ...201931121185
comparison of hematological, plasma biochemical, and nutritional analytes of rehabilitating and apparently healthy free-ranging atlantic green turtles (chelonia mydas).green turtles (chelonia mydas) are unique because hatchlings and pelagic juveniles are carnivorous, whereas later life stages become primarily herbivorous. although this dietary shift is well understood, nutritional requirements at each developmental stage are not. diet selection during rehabilitation is challenging, because turtles are often fed high-protein, low-fiber diets to counteract poor appetite and emaciation, which can result in gastrointestinal disorders. the objective of this study w ...201931120664
physiological changes in post-hatchling green turtles (chelonia mydas) following short-term fasting: implications for release protocols.relocation of sea turtle nests and the retention of post-hatchlings for head-starting programs are both commonly used to improve conservation outcomes and facilitate eco-tourism ventures. currently, there is little literature surrounding the husbandry protocols required during these programs to optimize post-release outcomes. to assess the impact of varied feeding regimes on exercise performance, (which will hereafter be referred to as 'fitness'), 40 10-month-old captive post-hatchling green tur ...201931110766
primary multicentric pulmonary low-grade fibromyxoid sarcoma and chelonid alphaherpesvirus 5 detection in a leatherback sea turtle (dermochelys coriacea).we describe the gross, microscopical, histochemical and immunohistochemical features of a sclerosing pneumopathic disease process resembling primary multicentric pulmonary low-grade fibromyxoid sarcoma in a juvenile female leatherback sea turtle (dermochelys coriacea). the animal was fresh, presented in good body condition and stranded dead in aracaju, sergipe state, brazil, in september, 2017. grossly, the lungs were enlarged bilaterally and the parenchyma was replaced by large, coalescing, whi ...201931103052
plasma bleomycin concentrations during electrochemotherapeutic treatment of fibropapillomas in green turtles chelonia mydas.fibropapillomatosis of sea turtles is traditionally treated with surgical debulking techniques that are often associated with prolonged healing and tumor recurrence. electrochemotherapy was recently described for green turtles chelonia mydas and can be an alternative to surgery and even general anesthesia. the objectives of this study were to replicate an electrochemotherapy protocol from a previous report and add plasma bleomycin analysis to the treatment. after bleomycin injection into similar ...201931100193
ultrasound examination of coelomic viscera through the plastron in stranded green sea turtles (chelonia mydas).many projects have been developed in the last years for the conservation of sea turtles. young green turtles (chelonia mydas) often nest on the brazilian coast. because they nest in beaches along the coastline and islands, green turtles are susceptible to fishing and accidental ingestion of anthropogenic debris. early detection of ingested debris is crucial for the survival of rescued sea animals. ultrasound (us) has emerged as a viable imaging technique for visceral examination in veterinary me ...201931086764
bacteriophage versus antibiotic therapy on gut bacterial communities of juvenile green turtle, chelonia mydas.green turtles are endangered marine herbivorous hindgut fermenters that contribute to a variety of marine ecosystems. debilitated turtles are often rehabilitated in turtle hospitals. since accurate diagnosis of disease is difficult, broad-spectrum antibiotics are routinely used as a general treatment, potentially causing collateral damage to the gut microbiome of the patient. here, we evaluated the concept of the application of bacteriophage (phages) to eliminate targeted intestinal bacteria as ...201931037801
characterizing stable isotope relationships between green turtle (chelonia mydas) skin and unhatched eggs.stable isotope analysis is used to understand the foraging habits and movements of a diverse set of organisms. variability in stable isotope ratios among tissues derived from the same animal makes it difficult to compare data among study results in which different tissue types are evaluated. isotopic relationships between two green turtle (chelonia mydas) tissue types, skin and unhatched egg contents are unknown. similarly, few data exist to evaluate the influence of time elapsed after ovipositi ...201931034695
densities and drivers of sea turtle populations across pacific coral reef ecosystems.sea turtle populations are often assessed at the regional to sub-basin scale from discrete indices of nesting abundance. while this may be practical and sometimes effective, widespread in-water surveys may enhance assessments by including additional demographics, locations, and revealing emerging population trends. here, we describe sea turtle observations from 13 years of towed-diver surveys across 53 coral islands, atolls, and reefs in the central, west, and south pacific. these surveys covere ...201931017916
genetic background of ctx-m-15-producing enterobacter hormaechei st114 and citrobacter freundii st265 co-infecting a free-living green turtle (chelonia mydas).ctx-m-type extended-spectrum β-lactamase (esbl)-producing enterobacteriaceae have become identified in marine ecosystem constituting a serious ecological issue. in this respect, although contamination of coastal waters and seafood, and even colonization of seabirds and fishes have been increasingly reported, molecular data are lacking to elucidate the clinical impact of esbl producers in infected marine animals. in this study, using a genomic approach, we have analysed the genetic background of ...201930843359
establishment and application of an evaluation model for orchid island sustainable tourism development.global warming and climate change increase the likelihood of weather-related natural disasters that threaten ecosystems and consequently affect the tourism industry which thrives on the natural attributes of island regions. orchid island, the study area, is home to the yami (tao) tribe-the only indigenous people of taiwan with a marine culture. the island possesses rich geological and topographical features (such as coral reefs) and distinctive biological and ecological resources (such as the gr ...201930832323
mixed stock analysis of juvenile green turtles aggregating at two foraging grounds in fiji reveals major contribution from the american samoa management unit.in this study we assessed the breeding population, or management unit (mu), origin of green turtles (chelonia mydas) present at yadua island and makogai island foraging grounds in fiji, central south pacific. based on analysis of mitochondrial (mt) dna sequences from 150 immature green turtles caught during surveys carried out in 2015-2016, we identified a total of 18 haplotypes, the most common being cmp22.1 (44%) which is a primary haplotype characterizing the american samoa breeding populatio ...201930816199
potential interaction between plastic litter and green turtle chelonia mydas during nesting in an extremely polluted beach.this study examines the extent of macroplastic pollution on samandağ beach and the potential effects on green sea turtles during nesting. for this purpose, a total of 39 different turtle tracks were studied. mean plastic concentration was found to be 19.5 ± 1.2 pcs m-2. among the different types of crawling, the highest concentrations of plastics were found on the tracks of turtles that did not attempt to dig nests (25.9 ± 8.4 pcs m-2). in total, 7 different types of plastics (disposable, film, ...201930803627
scavenging as a pathway for plastic ingestion by marine animals.plastic pollution is prevalent worldwide and affects marine wildlife from urbanized beaches to pristine oceanic islands. however, the ecological basis and mechanisms that result in marine animal ingestion of plastic debris are still relatively unknown, despite recent advances. we investigated the relationship between scavenging behavior and plastic ingestion using green turtles, chelonia mydas, as a model. diet analysis of c. mydas showed that sea turtles engaging in scavenging behavior ingested ...201930784834
trace metals in green sea turtles (chelonia mydas) inhabiting two southern california coastal estuaries.foraging aggregations of east pacific green sea turtles (chelonia mydas) inhabit the seal beach national wildlife refuge (sbnwr) and san diego bay (sdb), two habitats in southern california, usa, located near urbanized areas. both juvenile and adult green turtles forage in these areas and exhibit high site fidelity, which potentially exposes green turtles to anthropogenic contaminants. we assessed 21 trace metals (tm) bioaccumulated in green turtle scute and red blood cell (rbc) samples collecte ...201930784740
perspectives on the expansion of human precision oncology and genomic approaches to sea turtle fibropapillomatosis.our recent communications biology research article revealed the genomic drivers and therapeutic vulnerabilities of sea turtle fibropapillomatosis tumors. fibropapillomatosis is a debilitating tumorous disease afflicting populations of green sea turtles globally. while a virus is involved in the development of this disease, it is increasingly understood that the key trigger is linked to anthropogenic disturbances of the environment. the specific environmental co-trigger(s) has yet to be functiona ...201930775456
monitoring the health of green turtles in northern queensland post catastrophic events.between 2014 and 2017, the rivers to reef to turtles (rrt) project examined the health of green turtles at two coastal sites impacted by urban and agricultural human activities (cleveland and upstart bays) and one proposed pristine site (howick group of reefs) in northern queensland, australia, through blood biochemistry and haematology, plasma protein electrophoresis, and clinical assessments including body condition and barnacle counts. furthermore, cases of mortality were subjected to compreh ...201930641386
how many came home? evaluating ex situ conservation of green turtles in the cayman islands.ex situ management is an important conservation tool that allows the preservation of biological diversity outside natural habitats while supporting survival in the wild. captive breeding followed by re-introduction is a possible approach for endangered species conservation and preservation of genetic variability. the cayman turtle centre ltd was established in 1968 to market green turtle (chelonia mydas) meat and other products and replenish wild populations, thought to be locally extirpated, th ...201930636347
presence of chelonid herpesvirus 5 (chhv5) in sea turtles in northern sinaloa, mexico.the presence of fibropapilloma and its associated chelonid herpesvirus 5 (chhv5) was assessed in 82 wild sea turtles. olive ridley turtles lepidochelys olivacea (n = 58) were caught in the pelagic area of marine influence (ami) (off the coast of guasave, sinaloa), and black turtles chelonia mydas agassizii (n = 24) were captured in the navachiste lagoon system. the apparent physical condition was evaluated as 'good' or 'poor' by physical examination. the population structure and general health s ...201930628576
connecting paths between juvenile and adult habitats in the atlantic green turtle using genetics and satellite tracking.although it is commonly assumed that female sea turtles always return to the beach they hatched, the pathways they use during the years preceding their first reproduction and their natal origins are most often unknown, as it is the case for juvenile green turtles found in martinique waters in the caribbean. given the oceanic circulation of the guiana current flowing toward martinique and the presence of important nesting sites for this species in suriname and french guiana, we may assume that a ...201830619583
organochlorines contaminants in eggs of hawksbill (eretmochelys imbricata) and green sea turtles (chelonia mydas) from mexico coast.the investigation of organochlorine pesticides (ocps) levels in sea turtles is an important issue in conservation research, due to the harmful effects of these chemicals. in the present study, ocps concentrations were determined in the eggs of two sea turtle species (eretmochelys imbricata and chelonia mydas) collected from the punta xen and isla aguada (mexican coast) in 2014 and 2015. concentrations of 20 ocps were analysed, including isomers of hexachlorocyclohexane, aldrin, chlordanes, endos ...201930600338
remains of leatherback turtles, dermochelys coriacea, at mid-late holocene archaeological sites in coastal oman: clues of past worlds.small, irregular isolated bones identified as remains of leatherback turtles (dermochelys coriacea) were recovered from mid to late holocene sites at ra's al-hamra and ra's al-hadd, coastal oman. these provide the third instance of this animal being documented from any prehistoric site anywhere, and the records provide one of the oldest, if not the oldest, dates for this distinctive chelonian-even though they do not refer to fossils. decades of research in this region has yielded vast amounts of ...201830588406
green turtle (chelonia mydas) population demographics at three chemically distinct foraging areas in the northern great barrier reef.the catchments of the great barrier reef (gbr) have experienced significant modifications in recent decades, leading to increases in sources of pollutants and declines in coastal water quality. as coastal waters of the gbr support some of the highest density green turtle (chelonia mydas) foraging populations in the western pacific ocean, understanding the effects of contaminants on gbr green turtle populations is a priority. in 2012, elevated strandings of green turtles in the upstart bay region ...201930586791
evaluating internal exposure of sea turtles as model species for identifying regional chemical threats in nearshore habitats of the great barrier reef.marine megafauna that forage in proximity to land can be exposed to a diverse mixture of chemicals that - individually or combined - have the potential to affect their health. characterizing such complex exposure and examining associations with health still poses considerable challenges. the present study summarizes the development and application of novel approaches to identifying chemical hazards and their potential impacts on the health of coastal wildlife, using green sea turtles as model sp ...201930583168
climate change resilience of a globally important sea turtle nesting population.few studies have looked into climate change resilience of populations of wild animals. we use a model higher vertebrate, the green sea turtle, as its life history is fundamentally affected by climatic conditions, including temperature-dependent sex determination and obligate use of beaches subject to sea level rise (slr). we use empirical data from a globally important population in west africa to assess resistance to climate change within a quantitative framework. we project 200 years of primar ...201930567014
integrated chemical exposure assessment of coastal green turtle foraging grounds on the great barrier reef.the great barrier reef receives run-off from 424,000 km2 catchment area across coastal queensland, incorporating diffuse agricultural run-off, and run-off point sources of land-based chemical pollutants from urban and industrial development. marine biota, such as green turtles (chelonia mydas), are exposed to these diverse chemical mixtures in their natural environments, and the long term effects on turtle and ecosystem health remain unknown. this study was part of a larger multi-disciplinary pr ...201930550904
physical characteristics of free-living sea turtles that had and had not ingested debris in microregion of the lakes, brazil.the ingestion of detritus by sea turtles results in high mortality and morbidity. the objective of this study was to describe the characteristics of free-living sea turtles that ingested anthropogenic inorganic detritus in comparison to those that did not. a total of 186 necropsy files were analyzed in marine turtles from the beaches of the microregion dos lagos, rio de janeiro. among the turtles that ingested detritus, the mean turtle was female and cachectic, with a carapace length of 36,6 cm, ...201830503490
primary green turtle (chelonia mydas) skin fibroblasts as an in vitro model for assessing genotoxicity and oxidative stress.little is known about the effects of contaminants that accumulate in sea turtles. when in vivo exposure studies have ethical and logistical barriers, as is the case with sea turtles, in vitro tools can provide important information on the effects of contaminants. several in vitro studies have assessed cytotoxicity of contaminants to sea turtles cells, however to gain a more refined mechanistic understanding of the effects of contaminants, sub-lethal effects also require investigation. considerin ...201930502692
multi-residue screening of non-polar hazardous chemicals in green turtle blood from different foraging regions of the great barrier reef.green turtles spend a large part of their lifecycle foraging in nearshore seagrass habitats, which are often in close proximity to sources of anthropogenic contaminants. as most biomonitoring studies focus on a limited number of targeted chemical groups, this study was designed to screen for a wider range of hazardous chemicals that may not have been considered in prior studies. whole blood of sub-adult green turtles (chelonia mydas) were sampled from three different locations, a remote, offshor ...201930380492
first evidence of polychaete intermediate hosts for neospirorchis spp. marine turtle blood flukes (trematoda: spirorchiidae).life cycles of spirorchiids that infect the vascular system of turtles are poorly understood. few life cycles of these blood flukes have been elucidated and all intermediate hosts reported are gastropods (mollusca), regardless of whether the definitive host is a freshwater or a marine turtle. during a recent survey of blood fluke larvae in polychaetes on the coast of south carolina, usa, spirorchiid-like cercariae were found to infect the polychaetes amphitrite ornata (terebellidae) and enoplobr ...201830367866
elucidating temporal trends in trace element exposure of green turtles (chelonia mydas) using the toxicokinetic differences of blood and scute samples.blood is considered a suitable biomonitoring matrix for evaluating relatively recent exposure to environmental contaminants since abrupt changes in exposure regimes are rapidly reflected in blood. on the other hand, keratinized tissues, such as turtle scutes, are known to integrate trace element exposure over relatively long time periods. this study aimed to test the use of the differences in blood and scute to inform on the historical trace element exposure of green turtles. we propose a blood- ...201930336435
marine debris ingestion of green sea turtles, chelonia mydas, (linnaeus, 1758) from the eastern coast of the united arab emirates.the deleterious effects of marine debris ingestion on marine turtles are well documented in literature globally. in this study, the qualitative and quantitative aspects of marine debris ingested by 14 stranded green sea turtles chelonia mydas, (linnaeus, 1758) along the eastern coast of the united arab emirates were investigated. the numeric and gravimetric proportions of debris in the esophagus, stomach and intestines were documented following classification of color, presumed sources and marin ...201830301072
impacts of the traditional baited basket fishing trap "gargoor" on green sea turtles chelonia mydas (testudines: cheloniidae) linnaeus, 1758 from two case reports in the united arab emirates.the united arab emirates fisheries consist of highly diverse fish communities, and the most popular equipment used by fishermen to harvest them is a traditional baited basket fishing trap known locally as "gargoor". gargoors are dome-shaped traps made from galvanized steel; they have a circular supporting base and a funnel-like entrance. unintended impacts of gargoors on marine fauna include bycatch of non-target species and, when lost, ghost fishing. however, there is very little information on ...201830301068
the influence of temperature on embryonic respiration, growth, and sex determination in a western australian population of green turtles (chelonia mydas).the thermal environment of sea turtle embryos has marked effects on many aspects of their development and energetics and has consequences for posthatching stages. here we incubated chelonia mydas embryos from ningaloo reef in western australia at a range of temperatures (27°, 29°, 30°, 31°, 32°, and 30° ± 5°c) to determine development rates and the pivotal temperature for sex determination. we also measured embryonic growth, oxygen consumption, and carbon dioxide production throughout developmen ...201930289727
closing the gap: mixed stock analysis of three foraging populations of green turtles (chelonia mydas) on the great barrier reef.a solid understanding of the spatial ecology of green turtles (chelonia mydas) is fundamental to their effective conservation. yet this species, like many marine migratory species, is challenging to monitor and manage because they utilise a variety of habitats that span wide spatio-temporal scales. to further elucidate the connectivity between green turtle rookeries and foraging populations, we sequenced the mtdna control region of 278 turtles across three foraging sites from the northern great ...201830280029
colour vision of green turtle (chelonia mydas) hatchlings: do they still prefer blue under water?several anatomical studies provide evidence that green turtles (chelonia mydas) possess the necessary anatomy for colour vision. behavioural experiments have previously been conducted with newly emerged hatchlings, concluding that they are attracted to shorter wavelengths compared to longer wavelengths within a terrestrial environment, suggesting a possible attraction towards blue. this paper assessed the colour vision of hatchlings within an aquatic environment, and investigated whether the att ...201830258709
first record of the marine turtle leech (ozobranchus margoi) on hawksbill turtles (eretmochelys imbricata) in the inner granitic seychelles.ozobranchus spp. are leeches that feed solely on turtle blood. they are common ectoparasites found on a range of marine turtle species, with some species of the leech being implicated as vectors of fibropapilloma-associated turtle herpesvirus (fpthv). green (chelonia mydas) and hawksbill (eretmochelys imbricata) turtles are the two commonly occurring species in the inner granitic islands of the seychelles. routine monitoring of nesting turtles on cousine island, seychelles, allowed for opportuni ...201830198282
establishing a protocol for thromboelastography in sea turtles.thromboelastography (teg) provides a global evaluation of haemostasis. this diagnostic test is widely used in mammals but has not previously been performed in reptiles, mainly due to the limited availability of taxon-specific reagents. the objective of this pilot study was to establish a protocol to perform teg in sea turtles. pooled citrated plasma, stored at -80°c, from four green turtles (chelonia mydas) was assayed on a teg 5000. several initiators were evaluated: kaolin (n=2), rapidteg (n=2 ...201830167312
monitoring data of marine turtles on the togolese coast during 2012-2013.this dataset contains information on the presence and distribution of sea turtles in togo. observations were carried out through a network of ten ecoguards (local guides), facilitated by five fishermen, and coordinated by a field technician, all under the supervision of a scientific coordinator. data on the occurrence or direct observation of sea turtles on the togolese coast from september 2012 to august 2013 is presented based on 740 occurrences.201830123024
validation of an optimised protocol for quantification of microplastics in heterogenous samples: a case study using green turtle chyme.quantifying the extent of microplastic (<5 mm) contamination in the marine environment is an emerging field of study. reliable extraction of microplastics from the gastro-intestinal content of marine organisms is crucial to evaluate microplastic contamination in marine fauna. extraction protocols and variations thereof have been reported, however, these have mostly focussed on relatively homogenous samples (i.e. water, sediment, etc.). here, we present a microplastic extraction protocol for exam ...201830112289
fibropapillomatosis in a green sea turtle ( chelonia mydas) from the southeastern pacific.fibropapillomatosis is a neoplastic disease that afflicts sea turtles. although it is disseminated worldwide, cases of the disease have not been reported in the southeastern pacific region. we describe a case of fibropapillomatosis in a green sea turtle ( chelonia mydas) during its rehabilitation at the machalilla national park rehabilitation center, ecuador. viral presence was confirmed by pcr, targeting fragments of the chelonid alphaherpesvirus 5 (chhv5) unique long (ul) genes, ul27, ul28, an ...201930096036
individual specialization in a migratory grazer reflects long-term diet selectivity on a foraging ground: implications for isotope-based tracking.stable isotope analysis (sia) can be a useful tool for tracking the long-distance movements of migratory taxa. however, local-scale sources of isotopic variation, such as differences in habitat use or foraging patterns, may complicate these efforts. few studies have evaluated the implications of local-scale foraging specializations for broad-scale isotope-based tracking. here, we use > 300 h of animal-borne video footage from green turtles (chelonia mydas) paired with sia of multiple tissues, as ...201829992416
identification of bacteria present in ulcerative stomatitis lesions of captive sea turtles chelonia mydas.anthropogenic activities, predation, and diseases have contributed to a decrease in the sea turtle population in recent years. ulcerative stomatitis is a condition that occurs in both wild and captive populations. the etiology of this condition is associated with bacteria such as e. coli, citrobacter diversus, klebsiella spp., pseudomonas spp., flavobacter calcoaceticus, staphylococcus spp., and flavobacterium spp. some of these microorganisms are part of the oral microbiota of turtles, but alte ...201829934703
the risk of polychlorinated biphenyls facilitating tumors in hawaiian green sea turtles (chelonia mydas).the hawaiian green turtle (chelonia mydas) is on the list of threatened species protected under the u.s. endangered species act in 1978 in large part due to a severe tumor-forming disease named fibropapillomatosis. chemical pollution is a prime suspect threatening the survival of c. mydas. in this study, pcbs concentrations were determined in 43 c. mydas plasma samples archived on tern island. the total pcbs concentration in male c. mydas (mean 1.10 ng/ml) was two times more than that of females ...201829895772
relationship between metabolic heating and nest parameters in green turtles (chelonia mydas, l. 1758) on samandağ beach, turkey.acquiring information about metabolic heating is necessary for the improved understanding of nesting beaches. however, there are limited studies on metabolic heating of green turtle (chelonia mydas, l. 1758) on mediterranean beaches. the present study sought to determine the metabolic heating, and its effect on the feminization of green turtle hatchlings during 2016 and 2017 nesting season on samandağ beach, hatay, turkey. moreover, the relations between metabolic heating and nest parameters, an ...201829882496
concentrations of polycyclic aromatic hydrocarbons in liver samples of juvenile green sea turtles from brazil: can these compounds play a role in the development of fibropapillomatosis?fibropapillomatosis (fp) poses a significant threat to the conservation of green sea turtles (chelonia mydas). polycyclic aromatic hydrocarbons-pahs are considered mutagenic, carcinogenic and toxic, and can act as cofactor of this disease. in order to evaluate possible differences between green sea turtles with and without fp, we monitored 15 pahs in liver samples of 44 specimens (24 with fp) captured in brazil. we detected eight pahs and quantified phenanthrene in all green sea turtles with fp. ...201829866550
an outbreak of salmonella muenchen after consuming sea turtle, northern territory, australia, 2017.an outbreak of salmonella muenchen gastroenteritis occurred in a remote coastal aboriginal community in the northern territory (nt) of australia. there were 22 people sick (attack rate 55%); 7 had laboratory confirmed s. muenchen infection; 2 required medical evacuation and admission to the intensive care unit (icu). we conducted a descriptive case series to investigate the outbreak. all cases ate meat from a single green turtle (chelonia mydas). the animal's pre-death stress, improper butcherin ...201729864382
cadmium in tissues of green turtles (chelonia mydas): a global perspective for marine biota.cadmium (cd) is a metal of toxicological interest because of its potential high toxicity to organisms and ability to biomagnify. evaluating concentrations of cd in organisms on a large spatial scale can provide insights to its global distribution. this study examined cd concentrations in kidney and liver tissues of 137 specimens of green turtles (chelonia mydas) collected in australia, brazil, hawaii, japan, and the continental united states (gulf of mexico). we used comparative analyses of kidn ...201829753227
amphiorchis stacyi n. sp. (digenea: spirorchiidae) in the heart of a green turtle from florida, usa and the literature review of amphiorchis (price, 1934).the present paper reports the occurrence of the seventh species in the genus amphiorchis (digenea: spirorchiidae) collected from the heart of a green turtle found in florida, usa. a taxonomic key to the species of amphiorchis and a literature review for the genus are presented.201829696396
dna metabarcoding and microscopic analyses of sea turtles biofilms: complementary to understand turtle behavior.sea turtles are distributed in tropical and subtropical seas worldwide. they play several ecological roles and are considered important indicators of the health of marine ecosystems. studying epibiotic diatoms living on turtle shells suggestively has great potential in the study of turtle behavior because diatoms are always there. however, diatom identification at the species level is time consuming, requires well-trained specialists, and there is a high probability of finding new taxa growing o ...201829659610
assessing nesting status of green turtles, chelonia mydas in perak, malaysia.the nesting of green turtle (chelonia mydas) was monitored from 1998 untill 2013 along the beaches of pasir panjang, segari, perak. the objective of the study is to assess the nesting status of green turtles in perak, peninsular malaysia in terms of total nests, eggs, survival hatchings, and density of visitors. a total number of green turtle nests found for 16 years were 1,019 nests and varied from 10 to 220 nests per year. meanwhile, the sum of eggs collected for 16 years were 107,820 eggs, an ...201829644022
fast acquisition of a polysaccharide fermenting gut microbiome by juvenile green turtles chelonia mydas after settlement in coastal habitats.tetrapods do not express hydrolases for cellulose and hemicellulose assimilation, and hence, the independent acquisition of herbivory required the establishment of new endosymbiotic relationships between tetrapods and microbes. green turtles (chelonia mydas) are one of the three groups of marine tetrapods with an herbivorous diet and which acquire it after several years consuming pelagic animals. we characterized the microbiota present in the feces and rectum of 24 young wild and captive green t ...201829636094
discovery of an australian chelonia mydas papillomavirus via green turtle primary cell culture and qpcr.the number of reptilian viruses detected are continuously increasing due to improvements and developments of new diagnostic techniques. in this case we used primary cell culture and qpcr to describe the first australian chelonia mydas papillomavirus. commercial chelonian cell lines are limited to one cell line from a terrestrial turtle (terrapene carolina). to establish primary cultures from green turtles (chelonia mydas), turtle eggs were collected from heron island, queensland, australia. from ...201829630942
molecular identification of fungal isolates and hatching success of green turtle (chelonia mydas) nests.the aim of this study is to investigate the fungal diversity of green turtle nests and to examine phylogenetic relationships among these isolates. during the nesting season, samples of intra-nest sand and failed eggs were collected from 25% of the surviving nests in sugözü beaches, which are amongst the most important nesting beaches for endangered green turtles in the mediterranean. twenty-three fungi were identified by molecular techniques. fungal isolates belonged to genera aspergillus, emeri ...201829480404
ingestion of microplastic debris by green sea turtles (chelonia mydas) in the great barrier reef: validation of a sequential extraction protocol.ocean contamination by plastics is a global issue. although ingestion of plastic debris by sea turtles has been widely documented, contamination by microplastics (<5mm) is poorly known and likely to be under-reported. we developed a microplastic extraction protocol for examining green turtle (chelonia mydas) chyme, which is multifarious in nature, by modifying and combining pre-established methods used to separate microplastics from organic matter and sediments. this protocol consists of visual ...201829475719
differential impact of marine debris ingestion during ontogenetic dietary shift of green turtles in uruguayan waters.anthropogenic debris ingestion has been reported for green turtles in all their life stages worldwide. the aim of the present study is to evaluate the marine debris ingestion by green turtles stranded in uruguayan coast between 2005 and 2013. debris items were categorized and quantified by frequency of occurrence, relative weight, volume and number of items. a total of 96 dead stranded turtles were analyzed and 70% presented debris in their guts. the majority of debris found were plastic, being ...201829475703
comparative analysis of gut bacterial communities of green turtles (chelonia mydas) pre-hospitalization and post-rehabilitation by high-throughput sequencing of bacterial 16s rrna gene.stranded green turtles (chelonia mydas) are often cared for in rehabilitation centers until they recover. although the specific causal agents of diseases in stranded turtles are difficult to diagnose, we know that gut microbiota of green turtles play a vital role in health as well as a wide range of diseases. the objective of this study was to characterize and compare the gut bacterial communities between pre-hospitalization (ph) and post-rehabilitation (pr) stranded green turtles using high-thr ...201829458874
hematologic and biochemical characteristics of stranded green sea turtles.to improve understanding of pathophysiologic processes occurring in green sea turtles ( chelonia mydas) stranded along the east coast of australia, we retrospectively examined the hematologic and biochemical blood parameters of 127 green turtles admitted to 2 rehabilitation facilities, dolphin marine magic (dmm) and taronga zoo (tz), between 2002 and 2016. the predominant size class presented was small immature animals (sim), comprising 88% and 69% of admissions to dmm and tz, respectively. sign ...201829436286
environmental warming and feminization of one of the largest sea turtle populations in the world.climate change affects species and ecosystems around the globe [1]. the impacts of rising temperature are particularly pertinent in species with temperature-dependent sex determination (tsd), where the sex of an individual is determined by incubation temperature during embryonic development [2]. in sea turtles, the proportion of female hatchlings increases with the incubation temperature. with average global temperature predicted to increase 2.6°c by 2100 [3], many sea turtle populations are in ...201829316410
plasma cholinesterase activity in female green turtles chelonia mydas nesting in laguna de terminos, mexico related to organochlorine pesticides in their eggs.the inhibition of cholinesterase (che) activity has been used as a biomarker of exposure to organophosphate and carbamate insecticides. che of nesting female green turtles (chelonia mydas) were biochemically characterized using two substrates, acetylthiocholine iodide and butyrylthiocholine iodide, and three che inhibitors (eserine sulfate, bw284c51 and iso-ompa). the results indicated that bche is the predominant plasma che in female c. mydas, but with atypical properties that differ from those ...201829243208
comparison of whole blood and plasma glucose concentrations in green turtles ( chelonia mydas) determined using a glucometer and a dry chemistry analyzer.:  we compared glucose concentrations in whole blood and plasma from green turtles ( chelonia mydas) using a glucometer with plasma glucose analyzed by dry chemistry analyzer. whole blood glucose (glucometer) and plasma glucose (dry chemistry) had the best agreement ( rs=0.85) and a small negative bias (-0.08 mmol/l).201829087774
plasma lead, silicon and titanium concentrations are considerably higher in green sea turtle from the suburban coast than in those from the rural coast in okinawa, japan.the purpose of this study was to compare the concentration of trace elements in the plasma of sea turtles that inhabited the suburban (okinawa main island, n=8) and the rural coast (yaeyama island, n=57) in okinawa, japan. particle induced x-ray emission allowed detection of 20 trace and major elements. the wild sea turtles in the suburban coast in okinawa were found to have high concentrations of pb, si and ti in the plasma when compared to the rural area but there were no significant changes i ...201729070764
fecal bacterial communities of wild-captured and stranded green turtles (chelonia mydas) on the great barrier reef.green turtles (chelonia mydas) are endangered marine herbivores that break down food particles, primarily sea grasses, through microbial fermentation. however, the microbial community and its role in health and disease is still largely unexplored. in this study, we investigated and compared the fecal bacterial communities of eight wild-captured green turtles to four stranded turtles in the central great barrier reef regions that include bowen and townsville. we used high-throughput sequencing an ...201729069420
blue carbon stores in tropical seagrass meadows maintained under green turtle grazing.seagrass meadows are important sites for carbon storage. green turtles (chelonia mydas) are marine megaherbivores that consume seagrass throughout much of their global range. with successful conservation efforts, turtle abundance will increase, leading to more meadows being returned to their natural grazed state. there is concern this may lead to a loss of carbon stored in these systems, but the effects of green turtle grazing on seagrass ecosystem carbon dynamics have not been investigated. her ...201729051581
satellite tagging of rehabilitated green sea turtles chelonia mydas from the united arab emirates, including the longest tracked journey for the species.we collected movement data for eight rehabilitated and satellite-tagged green sea turtles chelonia mydas released off the united arab emirates between 2005 and 2013. rehabilitation periods ranged from 96 to 1353 days (mean = 437 ± 399 days). seven of the eight tagged turtles survived after release; one turtle was killed by what is thought to be a post-release spear gun wound. the majority of turtles (63%) used shallow-water core habitats and established home ranges between dubai and abu dhabi, t ...201728873453
stable isotope discrimination factors and between-tissue isotope comparisons for bone and skin from captive and wild green sea turtles (chelonia mydas).the ecological application of stable isotope analysis (sia) relies on taxa- and tissue-specific stable carbon (δ13 c) and nitrogen (δ15 n) isotope discrimination factors, determined with captive animals reared on known diets for sufficient time to reflect dietary isotope ratios. however, captive studies often prohibit lethal sampling, are difficult with endangered species, and reflect conditions not experienced in the wild.201728857312
effect-based approach for screening of chemical mixtures in whole blood of green turtles from the great barrier reef.organisms are exposed to mixtures of both known and unknown chemicals which are diverse and variable, and thus difficult and costly to characterise and monitor using traditional target analyses. the objective of this study was to validate and apply in vitro effect-based methods by which whole blood can be used to screen internal exposure to such complex chemical mixtures. for this study, we used whole blood of green sea turtles (chelonia mydas). to ensure the chemical mixture in blood is transfe ...201828854388
sex differences and the heritability of scute pattern abnormalities in the green sea turtle from the ogasawara archipelago, japan.although scute pattern abnormalities in sea turtle species are considered to be strongly correlated with survival rate, there is little information available regarding these abnormalities and the primary cause for their development is unclear. for the conservation of sea turtle species, accumulating basic knowledge of scute pattern abnormalities is a fundamental step towards a better understanding of the causes of these abnormalities. in the present study, we counted vertebral and costal scutes ...201728770677
when is embryonic arrest broken in turtle eggs?turtle embryos enter a state of arrested development in the oviduct, allowing the mother greater flexibility in her reproductive schedule. development recommences once eggs transition from the hypoxic oviduct to the normoxic nest. significant mortality can occur if turtle eggs are moved between 12 h and 20 d after oviposition, and this is linked to the recommencement of embryonic development. to better understand the timing of developmental arrest and to determine how movement-induced mortality ...201828636430
metals in blood and eggs of green sea turtles (chelonia mydas) from nesting colonies of the northern coast of the sea of oman.the green sea turtle (chelonia mydas) has been a species of global concern for decades. in this study, heavy metals (mercury: hg; cadmium: cd; lead: pb; copper: cu; and zinc: zn) were measured in blood and three egg fraction of green sea turtles nesting on the northern coast of sea of oman. heavy metals concentrations in blood, yolk, albumen, and egg shell ranged between 0.16-36.78, 0.006-33.88, 0.003-4.02, and 0.002-6.85 μg/g (ww), respectively. according to the results, all heavy metals found ...201728631031
non-targeted, high resolution mass spectrometry strategy for simultaneous monitoring of xenobiotics and endogenous compounds in green sea turtles on the great barrier reef.chemical contamination poses a threat to ecosystem, biota and human health, and identifying these hazards is a complex challenge. traditional hazard identification relies on a priori-defined targets of limited chemical scope, and is generally inappropriate for exploratory studies such as explaining toxicological effects in environmental systems. here we present a non-target high resolution mass spectrometry environmental monitoring study with multivariate statistical analysis to simultaneously d ...201728521388
characterization of the juvenile green turtle (chelonia mydas) microbiome throughout an ontogenetic shift from pelagic to neritic habitats.the gut microbiome of herbivorous animals consists of organisms that efficiently digest the structural carbohydrates of ingested plant material. green turtles (chelonia mydas) provide an interesting model of change in these microbial communities because they undergo a pronounced shift from a surface-pelagic distribution and omnivorous diet to a neritic distribution and herbivorous diet. as an alternative to direct sampling of the gut, we investigated the cloacal microbiomes of juvenile green tur ...201728493980
shared epizoic taxa and differences in diatom community structure between green turtles (chelonia mydas) from distant habitats.the first reports of diatoms growing on marine mammals date back to the early 1900s. however, only recently has direct evidence been provided for similar associations between diatoms and sea turtles. we present a comparison of diatom communities inhabiting carapaces of green turtles chelonia mydas sampled at two remote sites located within the indian (iran) and atlantic (costa rica) ocean basins. diatom observations and counts were carried out using scanning electron microscopy. techniques invol ...201728477173
evidence of antibiotic resistance in enterobacteriales isolated from green sea turtles, chelonia mydas on the great barrier reef.this study investigated enterobacteriales and their antimicrobial resistance in green sea turtles captured adjacent to the central great barrier reef (gbr) and proximate to urban development. cloacal swabs were taken from 73 green turtles between 2015 and 2016. a total of 154 out of 341 gram-negative bacterial isolates were identified as enterobacteriales that represent 16 different species from 9 different genera. the dominant isolates were citrobacter (30.52%), edwardsiella (21.43%) and escher ...201728476351
distribution pattern of anthropogenic marine debris along the gastrointestinal tract of green turtles (chelonia mydas) as implications for rehabilitation.pollution from anthropogenic marine debris (amd) is currently the most widely distributed and lasting anthropic impact in the marine environment, affecting hundreds of species, including all sea turtles. in this study, the patterns of amd distribution along the gastrointestinal tract (gt) and their relationship with obstructions and faecalomas in 62 green turtles (chelonia mydas) that died during rehabilitation in southern brazil were determined. the gt was split in seven sections, corresponding ...201728408048
olfactory receptor subgenomes linked with broad ecological adaptations in sauropsida.olfactory receptors (ors) govern a prime sensory function. extant birds have distinct olfactory abilities, but the molecular mechanisms underlining diversification and specialization remain mostly unknown. we explored or diversity in 48 phylogenetic and ecologically diverse birds and 2 reptiles (alligator and green sea turtle). or subgenomes showed species- and lineage-specific variation related with ecological requirements. overall 1,953 or genes were identified in reptiles and 16,503 in birds. ...201526219582
first approximation to congenital malformation rates in embryos and hatchlings of sea turtles.congenital malformations in sea turtles have been considered sporadical. research carried out in the mexican pacific revealed high levels of congenital malformations in the olive ridley, but little or no information is available for other species. we present results from analyses of external congenital malformations in olive ridley, green, and hawskbill sea turtles from mexican rookeries on the pacific coast and gulf of mexico.201525761253
potential impacts of historical disturbance on green turtle health in the unique & protected marine ecosystem of palmyra atoll (central pacific).palmyra atoll, in the central pacific, is a unique marine ecosystem because of its remarkably intact food web and limited anthropogenic stressors. however during world war ii the atoll was structurally reconfigured into a military installation and questions remain whether this may have impacted the health of the atoll's ecosystems and species. to address the issue we assessed green sea turtle (n=157) health and exposure to contaminants at this foraging ground from 2008 to 2012. physical exams we ...201425455822
mistaken identity? visual similarities of marine debris to natural prey items of sea turtles.there are two predominant hypotheses as to why animals ingest plastic: 1) they are opportunistic feeders, eating plastic when they encounter it, and 2) they eat plastic because it resembles prey items. to assess which hypothesis is most likely, we created a model sea turtle visual system and used it to analyse debris samples from beach surveys and from necropsied turtles. we investigated colour, contrast, and luminance of the debris items as they would appear to the turtle. we also incorporated ...201424886170
evaluation of mercury mediated in vitro cytotoxicity among cell lines established from green sea turtles.in vitro cell cultures are currently tested for their application as a biological tool for enhanced monitoring and field evaluation of environmental toxic chemical pollution. here cell lines established from green sea turtles (gsts) were comparatively tested for their cytotoxic responses to mercury chloride (hgcl2) exposure and also their potential use as a biological tool for effective monitoring and screening of mercury contamination in environmental waters. following a 24-h exposure to differ ...201323380703
targeted multiplex next-generation sequencing: advances in techniques of mitochondrial and nuclear dna sequencing for population genomics.next-generation sequencing (ngs) is emerging as an efficient and cost-effective tool in population genomic analyses of nonmodel organisms, allowing simultaneous resequencing of many regions of multi-genomic dna from multiplexed samples. here, we detail our synthesis of protocols for targeted resequencing of mitochondrial and nuclear loci by generating indexed genomic libraries for multiplexing up to 100 individuals in a single sequencing pool, and then enriching the pooled library using custom d ...201323351075
the biology and ecology of black corals (cnidaria: anthozoa: hexacorallia: antipatharia).antipatharians, commonly known as black corals, are treasured by many cultures for medicinal purposes and to produce jewellery. despite their economic and cultural importance, very little is known about the basic biology and ecology of black corals because most species inhabit deeper-water environments (>50m) which are logistically challenging to study. there has been a recent increase of studies focusing on antipatharians; however, these have not yet been comprehensively reviewed. this literatu ...201222877611
death in the octopus' garden: fatal blue-lined octopus envenomations of adult green sea turtles.the blue-lined octopus hapalochlaena fasciata contains the powerful neuromuscular blocker tetrodotoxin (ttx), which causes muscle weakness and respiratory failure. h. fasciata is regarded as one of the most venomous marine animals in the world, and multiple human fatalities have been attributed to the octopus. to date, there have been no recorded incidents of an envenomation of a wild animal. here, we present a newly developed, multi-stage tandem mass spectrometry technique that provides unequiv ...201224391271
effects of biotic and abiotic factors on the oxygen content of green sea turtle nests during embryogenesis.several biotic and abiotic factors can influence nest oxygen content during embryogenesis. several of these factors were determined during each developmental stage of green sea turtle embryos on wan-an island, penghu archipelago, taiwan. we examined oxygen content in 7 nests in 2007 and 11 in 2008. oxygen in the adjacent sand, total and viable clutch sizes, air, sand and nest temperatures, and sand characters of each nest were also determined. oxygen content was lower in late stages than in the ...201020480166
pathology of oropharyngeal fibropapillomatosis in green turtles chelonia mydas.complete gross and histopathologic examinations of the oral cavity, tongue, pharynx, larynx, and glottis were performed in five hawaiian green turtles chelonia mydas with fibropapillomatosis. these examinations demonstrated that the oropharyngeal fibropapillomas were similar to characteristic external fibropapillomas previously described for green turtles. the size, appearance, and anatomic site of the tumors confirmed that these turtles presented total or partial occlusion of the nasopharynx, g ...200228880789
global population structure and natural history of the green turtle (chelonia mydas) in terms of matriarchal phylogeny.to address aspects of the evolution and natural history of green turtles, we assayed mitochondrial (mt) dna genotypes from 226 specimens representing 15 major rookeries around the world. phylogenetic analyses of these data revealed (1) a comparatively low level of mtdna variability and a slow mtdna evolutionary rate (relative to estimates for many other vertebrates); (2) a fundamental phylogenetic split distinguishing all green turtles in the atlantic-mediterranean from those in the indian-pacif ...199228564410
green sea turtles: a discrete simulation of density-dependent population regulation.field data on the nesting of the green sea turtle were used to construct a stochastic model. this model was simulated by use of a digital computer language simscript (monte carlo) to determine the relation between the percentage of nests destroyed and the size of the turtle population. nest destruction is dependent on population density and provides a mechanism to regulate population size.196917737318
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