Publications

development and validation of a range of endogenous controls to support the implementation of practical taqman real-time pcr-based surveillance for fish diseases within aquaculture.	the use of taqman real-time pcr-based technology has recently become more frequent in the detection of pathogens in the aquaculture industry. this interest has necessitated the development of robust and reliable pathogen-detection assays. the development of a range of endogenous control assays to be run alongside these diagnostic assays works to further increase confidence in the latter. this study describes the design of a range of endogenous control assays based on the elongation factor 1-α (e ...	2012	22524565
occurrence and molecular characterization of hysterothylacium aduncum (nematoda: anisakidae) from merlangius merlangus euxinus and trachurus trachurus off the turkish coast of black sea.	a total of 286 larval forms of hysterothylacium aduncum were collected from merlangius merlangus euxinus and trachurus trachurus captured at different sites of the black sea coast of turkey. prevalence of h. aduncum in m. merlangus euxinus and t. trachurus was 37.4 and 29.3 %, respectively. the fourth-stage larvae from m. merlangus euxinus and t. trachurus of h. aduncum were characterized genetically using a molecular approach. the ribosomal dna (rdna) internal transcribed spacer (its) region (i ...	2013	23239095
biochemical characterisation of alaska pollock, pacific whiting, and threadfin bream surimi as affected by comminution conditions.	salt-soluble protein, surface reactive sulfhydryl content, and surface hydrophobicity of alaska pollock, pacific whiting, and threadfin bream surimi were characterised, as affected by various comminution conditions. chopping time/temperatures were explored in consideration of their habitat temperatures. salt-soluble protein (ssp) significantly decreased when chopping time was extended. corresponding to our follow-up study, no relationship between ssp and gel texture was found. surface hydrophobi ...	2013	23265477
[the study on the influence of bovine enamel hardness measurement methods on the result evaluation].	the variation in hardness of enamel is a frequently used method to evaluate the influence of whiting materials on the enamel. the purpose of this study is to improve the veracity on the evaluation tests caused by the tooth itself with point selection method. three kinds of testing point selection methods on enamel were carried out, i. e. random selection, grid measurement and symmetrical measurement, respectively. the selected points were used to measure the micro hardness by vickers. the influe ...	2013	23488150
role of kairomones in host location of the pennellid copepod parasite, lernaeocera branchialis (l. 1767).	the life cycle of the parasitic copepod lernaeocera branchialis involves 2 hosts, typically a pleuronectiform host upon which development of larvae and mating of adults occurs and a subsequent gadoid host, upon which the adult female feeds and reproduces. both the copepodid and adult female stages must therefore locate and identify a suitable host to continue the life cycle. several mechanisms are potentially involved in locating a host and ensuring its suitability for infection. these may inclu ...	2013	23369461
a statistical model for estimation of fish density including correlation in size, space, time and between species from research survey data.	trawl survey data with high spatial and seasonal coverage were analysed using a variant of the log gaussian cox process (lgcp) statistical model to estimate unbiased relative fish densities. the model estimates correlations between observations according to time, space, and fish size and includes zero observations and over-dispersion. the model utilises the fact the correlation between numbers of fish caught increases when the distance in space and time between the fish decreases, and the correl ...	2014	24911631
screening for viral hemorrhagic septicemia virus in marine fish along the norwegian coastal line.	viral hemorrhagic septicemia virus (vhsv) infects a wide range of marine fish species. to study the occurrence of vhsv in wild marine fish populations in norwegian coastal waters and fjord systems a total of 1927 fish from 39 different species were sampled through 5 research cruises conducted in 2009 to 2011. in total, vhsv was detected by rrt-pcr in twelve samples originating from atlantic herring (clupea harengus), haddock (melanogrammus aeglefinus), whiting (merlangius merlangus) and silvery ...	2014	25248078
ecology and genetic structure of zoonotic anisakis spp. from adriatic commercial fish species.	consumption of raw or thermally inadequately treated fishery products represents a public health risk, with the possibility of propagation of live anisakis larvae, the causative agent of the zoonotic disease anisakidosis, or anisakiasis. we investigated the population dynamics of anisakis spp. in commercially important fish-anchovies (anisakis), sardines (sardina pilchardus), european hake (merluccius merluccius), whiting (merlangius merlangus), chub mackerel (scomber japonicus), and atlantic bl ...	2014	24317085
thermodynamic analysis of calcium binding to frog parvalbumin.	the enthalpy of calcium binding to frog parvalbumin (rana temporaria isoenzyme ivb, pi 4.75) has been measured by microcalorimetry. the reaction is exothermic; the heat of the reaction in 100 mm kcl, 50mm tris, ph 8.0 at 12 degrees c is -19 kj (mol site)-1 and -33 kj (mol site)-1 in the presence of 1 mm magnesium. the shape of the titration curve indicates that the properties of the two calcium binding sites are different. the thermodynamic parameters measured for frog parvalbumin are compared w ...	2014	3879254
grillotia erinaceus (van beneden, 1858) (cestoda:trypanorhyncha) from whiting in the black sea, with observations on seasonality and host-parasite interrelationship.	the genus grillotia guiart, 1927 is cosmopolitan in its distribution and the type-species, g. erinaceus (van beneden, 1858), has been relatively well studied. however, this study provides infection indices of grillotia erinaceus from southern and northern black sea whiting merlangius merlangus for the first time. the specimens of grillotia erinaceus were obtained from subserosa of the anterior oesophagus, stomach, pyloric caeca, liver, ovaries and mesenterium of whiting caught by commercial fish ...	2014	25119355
survey of viral haemorrhagic septicaemia virus in wild fishes in the southeastern black sea.	species diversity in the black sea ecosystem has been declining rapidly over the last 2 decades. to assess the occurrence and distribution of viral haemorrhagic septicaemia virus (vhsv) in various wild fish species, a wild marine fish survey was carried out in 2009, 2010, and 2011. the pooled or individual samples of kidney, liver, and spleen of 5025 specimens, belonging to 17 fish species, were examined virologically using cell culture. the cells showing cytopathic effects (cpe) were subjected ...	2014	24991737
phenotypic and genotypic characterization of h2 s-positive and h2 s-negative strains of shewanella baltica isolated from spoiled whiting (merlangius merlangus).	four strains were isolated from a spoiled whiting (merlangius merlangus). all of them were able to grow aerobically from 4 to 30°c and also able to develop anaerobically in the presence of trimethylamine n-oxide (tmao) at 25°c. biochemical characterization did not allow identification of the strains species but showed that one of the four strains was unable to produce h2 s. two strains synthetized an ornithine decarboxylase being potential putrescine producers. results of carbon source use highl ...	2014	25073930
andrographolide suppresses melanin synthesis through akt/gsk3β/β-catenin signal pathway.	tyrosinase (tyr) is the key enzyme controlling the production of melanin. very few papers have reported that andrographolide can inhibit melanin content.	2015	25869056
trace metals in tissues of the six most common fish species in the black sea, turkey.	concentrations of mn, fe, co, ni, as, se, cd, ag and pb in scale, skin, muscle, gills, liver and the gonads of gilthead seabream (sparus aurata), european seabass (dicentrarchus labrax), black sea salmon (salmo trutta labrax), horse mackerel (trachurus trachurus), red mullet (mullus barbatus) and whiting (merlangius merlangius euxinus) from the black sea, in turkey, were investigated. elemental analyses were performed with inductively coupled plasma-mass spectrometry after sample preparation by ...	2015	25082436
sexual size dimorphism in three north sea gadoids.	existing biological data on whiting merlangius merlangus, cod gadus morhua and haddock melanogrammus aeglefinus from a long-term international survey were analysed to address sexual size dimorphism (ssd) and its effect on their ecology and management. results show that ssd, with larger females of the same age as males, is a result of higher growth rates in females. a direct consequence of ssd is the pronounced length-dependent female ratio that was found in all three gadoids in the north sea. fe ...	2015	25457726
fatty acid and cholesterol concentrations in usually consumed fish in brazil.	several studies have demonstrated clinical benefits of fish consumption for the cardiovascular system. these effects are attributed to the increased amounts of polyunsaturated fatty acids in these foods. however, the concentrations of fatty acids may vary according to region.	2015	25424160
salmon blood plasma: effective inhibitor of protease-laden pacific whiting surimi and salmon mince.	the effect of salmon plasma (sp) from chinook salmon on proteolytic inhibition was investigated. sp was found to inhibit both cysteine and serine proteases as well as protease extracted from pacific whiting muscle. sp was found to contain a 55kda cysteine protease inhibitor through sds-page inhibitor staining. freeze dried salmon plasma (fsp) and salmon plasma concentrated by ultrafiltration (csp) were tested for their ability to inhibit autolysis in pacific whiting surimi and salmon mince at co ...	2015	25624255
a survey of wild marine fish identifies a potential origin of an outbreak of viral haemorrhagic septicaemia in wrasse, labridae, used as cleaner fish on marine atlantic salmon, salmo salar l., farms.	viral haemorrhagic septicaemia virus (vhsv) was isolated from five species of wrasse (labridae) used as biological controls for parasitic sea lice predominantly, lepeophtheirus salmonis (krøyer, 1837), on marine atlantic salmon, salmo salar l., farms in shetland. as part of the epidemiological investigation, 1400 wild marine fish were caught and screened in pools of 10 for vhsv using virus isolation. eleven pools (8%) were confirmed vhsv positive from: grey gurnard, eutrigla gurnardus l.; atlant ...	2015	25102953
revision of the oriental subfamily heteropteryginae kirby, 1896, with a re-arrangement of the family heteropterygidae and the descriptions of five new species of haaniella kirby, 1904. (phasmatodea: areolatae: heteropterygidae).	the areolate oriental family heteropterygidae kirby, 1893 is critically reviewed and the results of the present study contradict the arrangement suggested by zompro (2004), but in most aspects agree with a molecular study presented by whiting et al (2003) and a phylogenetic study presented by bradler (2009). the family is critically discussed and new hypotheses are presented for the phylogeny and intra-familiar relationships, placing the subfamily dataminae rehn & rehn, 1939 as the basalmost cla ...	2016	27615907
response of branchial na(+)/k(+) atpase to changes in ambient temperature in atlantic cod (gadus morhua) and whiting (merlangius merlangus).	the maintenance of ion and ph homeostasis despite changes in ambient temperature is crucial for ectothermic organisms. thermal sensitivity of na(+)/k(+) atpase mrna expression, protein expression and activity was determined in gills of north sea cod (nc) and northeastern arctic cod (neac), acclimated for 6 weeks at 4 and 10 °c and compared to field samples of north sea cod (snc), acclimatized to early spring (4 °c) and summer (18 °c) conditions. the same analyses were conducted in gills of the c ...	2016	26922791
modelling gastric evacuation in gadoids feeding on crustaceans.	a mechanistic, prey surface-dependent model was expanded to describe the course and rate of gastric evacuation in predatory fishes feeding on crustacean prey with robust exoskeletons. this was accomplished by adding a layer of higher resistance to the digestive processes outside the inner softer parts of a prey cylinder abstraction and splitting up the prey evacuation into two stages: an initial stage where the exoskeleton is cracked and a second where the prey remains are digested and evacuated ...	2016	27170110
preliminary investigation of perfluoroalkyl substances in exploited fishes of two contaminated estuaries.	perfluoroalkyl substances (pfass) are being increasingly detected in a range of aquatic and terrestrial ecosystems, often resulting from the use of legacy fire-fighting foams. this study conducted an initial investigation of the concentrations of pfass in the commercially and recreationally exploited species dusky flathead, mud crab, school prawn, sea mullet, yellowfin bream, eastern king prawn and sand whiting, across two contaminated estuaries. all samples contained perfluoro-n-octane sulfonat ...	2016	27289281