Publications

bioconcentration and multi-biomarkers of organic uv filters (bm-dbm and od-paba) in crucian carp.	organic uv filters (ouv-fs) are increasingly used in sunscreens and personal care products. in the present work, the bioconcentration and multi-biomarker effects of butyl methoxydibenzoylmethane (bm-dbm) and ethylhexyl dimethyl p-aminobenzoate (od-paba) were investigated in crucian carp (carassius auratus). the fish were exposed to various concentrations of bm-dbm (3.88, 35.61, 181.85 and 337.15μg/l), od-paba (4.66, 53.83, 264.22 and 459.32μg/l) and their mixture (2.31+2.79, 23.69+26.18, 97.37+1 ...	2017	28343007
ghrelin facilitates glut2-, sglt1- and sglt2-mediated intestinal glucose transport in goldfish (carassius auratus).	glucose homeostasis is an important biological process that involves a variety of regulatory mechanisms. this study aimed to determine whether ghrelin, a multifunctional gut-brain hormone, modulates intestinal glucose transport in goldfish (carassius auratus). three intestinal glucose transporters, the facilitative glucose transporter 2 (glut2), and the sodium/glucose co-transporters 1 (sglt1) and 2 (sglt2), were studied. immunostaining of intestinal sections found colocalization of ghrelin and ...	2017	28338019
influence of multi-walled carbon nanotubes on the effects of roxithromycin in crucian carp (carassius auratus) in the presence of natural organic matter.	carbon nanotubes are increasingly entering the aquatic environment and may interact with other co-existing contaminants, such as antibiotics. however, whether these interactions may affect their bioavailability in aquatic organisms is the subject of considerable debate. the primary objective of this study was to assess the risks arising from the coexistence of roxithromycin (rox) and multi-walled carbon nanotubes (mwcnts) in waters containing natural organic matter (nom), focusing on the distrib ...	2017	28324838
diverse chlamydia-like agents associated with epitheliocystis infection in two cyprinid fish species, the common carp (cyprinus carpio l.) and the gibel carp (carassius auratus gibelio l.).	during a general annual fish health survey in natural waters and ponds, epitheliocystis infections were recorded in fingerlings of two cyprinid fish species, the cultured common carp and the wild gibel carp. benign and heavy infections were equally observed without mortality. in addition to the general health inspection of fish, histopathological examinations of infected gills and molecular biological investigations of separated epitheliocysts were performed. epitheliocysts were formed both in t ...	2017	28244339
effects on heavy metal accumulation in freshwater fishes: species, tissues, and sizes.	three fish species (carassius auratus, pelteobagrus fulvidraco, and squaliobarbus curriculus) were collected from xiang river near changsha city, southern china. the concentrations of heavy metals including arsenic (as), cadmium (cd), copper (cu), iron (fe), manganese (mn), lead (pb), and zinc (zn) in the muscle, gill, and liver of three species were determined by the inductively coupled plasma mass spectrometry method. one-way analysis of variance (anova) was introduced to determine the signifi ...	2017	28233209
irisin in goldfish (carassius auratus): effects of irisin injections on feeding behavior and expression of appetite regulators, uncoupling proteins and lipoprotein lipase, and fasting-induced changes in fndc5 expression.	irisin is a peptide cleaved from the fibronectin type iii domain containing protein 5 (fndc5) gene that is secreted predominantly by muscle cells but also by other tissues including brain and intestine. in mammals, irisin has been shown to have thermogenic actions via the modulation of uncoupling proteins (ucps) and to affect feeding and energy homeostasis via actions in brain, adipose tissue, liver, muscle and gastrointestinal tract. to examine the role of irisin on feeding and metabolism in fi ...	2017	28219696
identification of the putative goldfish (carassius auratus) magnesium transporter slc41a1 and functional regulation in the gill, kidney, and intestine in response to dietary and environmental manipulations.	while magnesium requirements for teleost fish highlight the physiological importance of this cation for homeostasis, little is known regarding the molecular identity of transporters responsible for magnesium absorption or secretion. the recent characterization of the vertebrate magnesium transporter solute carrier 41a1 (slc41a1) in the kidney of a euryhaline fish has provided a glimpse of possible moieties involved in piscine magnesium regulation. the present study obtained a novel slc41a1 codin ...	2017	28130070
ifna2 of triploid hybrid of gold fish and allotetraploid is an intracellular antiviral cytokine against svcv and gcrv.	sterile triploid hybrids (3n = 150) of gold fish (carassius auratus red var., ♀, 2n = 100) and allotetroploid (♂, 2n = 100) display obviously improved disease resistance and much enhanced growth rate than their parents, which have been cultured widely in china. in this paper, one of the type i ifns of triploid hybrid (3nifna2) has been cloned and characterized. the full-length cdna of 3nifna2 gene consists of 715 nucleotides and the predicted 3nifna2 contains 183 amino acids. the transcription o ...	2017	28126620
proteome response of fish under multiple stress exposure: effects of pesticide mixtures and temperature increase.	aquatic systems can be subjected to multiple stressors, including pollutant cocktails and elevated temperature. evaluating the combined effects of these stressors on organisms is a great challenge in environmental sciences. to the best of our knowledge, this is the first study to assess the molecular stress response of an aquatic fish species subjected to individual and combined pesticide mixtures and increased temperatures. for that, goldfish (carassius auratus) were acclimated to two different ...	2017	28109940
composition of gut microbiota in the gibel carp (carassius auratus gibelio) varies with host development.	to understand how a bacteria-free fish gut ecosystem develops microbiota as the fish ages, we performed a 1-year study on the gut microbiota of hatchling gibel carp (carassius auratus gibelio). our results indicate that the gut microbial diversity increases significantly as the fish develop. the gut microbial community composition showed significant shifts corresponding to host age and appeared to shift at two time points despite consistent diet and environmental conditions, suggesting that some ...	2017	28108758
ghrelin modulates gene and protein expression of digestive enzymes in the intestine and hepatopancreas of goldfish (carassius auratus) via the ghs-r1a: possible roles of plc/pkc and ac/pka intracellular signaling pathways.	ghrelin, a multifunctional gut-brain hormone, is involved in the regulation of gastric functions in mammals. this study aimed to determine whether ghrelin modulates digestive enzymes in goldfish (carassius auratus). immunofluorescence microscopy found colocalization of ghrelin, ghs-r1a and the digestive enzymes sucrase-isomaltase, aminopeptidase a, trypsin and lipoprotein lipase in intestinal and hepatopancreatic cells. in vitro ghrelin treatment in intestinal and hepatopancreas explant culture ...	2017	28042022
microcystin bioaccumulation in freshwater fish at different trophic levels from the eutrophic lake chaohu, china.	the spatial and temporal variations of microcystins (mcs) in fishes with different trophic levels were studied monthly in lake chaohu in 2014. mcs content in muscle was highest in phytoplanktivorous hypophthalmichthys molitrix (h. molitrix), followed by omnivorous carassius auratus (c. auratus), and was lowest in herbivorous parabramis pekinensis (p. pekinensis) and carnivorous coilia ectenes (c. ectenes). mcs concentration in liver was highest in c. auratus, followed by h. molitrix, and was low ...	2017	28213674
allometric analysis of a morphological anti-predator trait in geographic populations of japanese crucian carp.	costly anti-predator traits tend to be expressed only in high-predation conditions. for the cyprinid fish genus carassius, deeper body depth is more adaptive to avoid predation by gape-limited piscivorous fish, but it raises swimming costs. it is therefore predicted that the relative body depth will decrease when the prey fish has reached a size larger than the predator gape-size. this prediction was tested by allometric analysis of the relation between body depth and standard length of triploid ...	2017	28150742
cryosurvival of isolated testicular cells and testicular tissue of tench tinca tinca and goldfish carassius auratus following slow-rate freezing.	experiments were carried out to test the efficiency of cryopreservation of whole testicular tissue in tench tinca tinca and goldfish carassius auratus and compare it to cryopreservation of isolated testicular cells. additionally, effects of three cryoprotectants (dimethyl sulphoxyde - me2so, methanol - meoh and ethylene glycol - eg) at three concentrations (1m, 2m and 3m) on post-thaw cell viability were assessed. tissue pieces/isolated testicular cells were diluted in cryomedia and cryopreserve ...	2017	27401260
toxicity and accumulation of zinc pyrithione in the liver and kidneys of carassius auratus gibelio: association with p-glycoprotein expression.	zinc pyrithione (zpt) is a broad-spectrum antibacterial and antifungal agent; therefore, it is widely used in industry and civilian life. it is discharged into the aquatic environment with industrial and civilian waste water. carassius sp. is one of the most widely distributed and farmed fish in china. the effects of aquatic zpt on carassius sp. remain unknown. in this study, we determined the acute toxicity of zpt on carassius sp. the results showed that the median lethal concentration (lc50 96 ...	2017	27387320
brain and intestinal expression of galanin-like peptide (galp), galanin receptor r1 and galanin receptor r2, and galp regulation of food intake in goldfish (carassius auratus).	galanin-like peptide (galp) is a 60 amino acid neuropeptide originally discovered from porcine hypothalamus, and is involved in the regulation of food intake in mammals. since its discovery, galp and its receptors (galr1 and galr2) have been characterized in mammals, but no publications are available on galp in fish and other non-mammals. the present study aimed to characterize brain and intestinal galp and its receptors using immunohistochemistry in a teleost, the goldfish (carassius auratus), ...	2017	27884736
determination of the gut retention of plastic microbeads and microfibers in goldfish (carassius auratus).	microplastics are ubiquitous pollutants in aquatic habitats and commonly found in the gut contents of fish yet relatively little is known about the retention of these particles by fish. in this study, goldfish were fed a commercial fish food pellet amended with 50 particles of one of two microplastics types, microbeads and microfibers. microbeads were obtained from a commercial facial cleanser while microfibers were obtained from washed synthetic textile. following consumption of the amended pel ...	2017	27880921
spontaneous polyploidy, gynogenesis and androgenesis in second generation (f2 ) koi cyprinus carpio × goldfish carassius auratus hybrids.	the objective of this study was to characterize the genetics of second generation (f2 ) koi cyprinus carpio × goldfish carassius auratus hybrids. spermatozoa produced by a novel, fertile f1 male were found to be diploid by flow-cytometric analysis. backcross (f1 female × c. carpio male and c. carpio female × f1 male) juveniles were triploid, confirming that female and male f1 hybrids both produced diploid gametes. the vast majority of surviving f2 juveniles was diploid and small proportions were ...	2017	27861861
nmr elucidation of reduced b-z transition activity of pkz protein kinase at high nacl concentration.	a z-dna binding protein (zbp)-containing protein kinase (pkz) in fish species has an important role in the innate immune response. previous structural studies of the zα domain of the pkz from carassius auratus (cazαpkz) showed that the protein initially binds to b-dna and induces b-z transition of double stranded dna in a salt concentration-dependent manner. however, the significantly reduced b-z transition activity of cazαpkz at high salt concentration was not fully understood. in this study, w ...	2017	27856245
stellate and pyramidal neurons in goldfish telencephalon respond differently to anoxia and gaba receptor inhibition.	with oxygen deprivation, the mammalian brain undergoes hyper-activity and neuronal death while this does not occur in the anoxia-tolerant goldfish (carassius auratus). anoxic survival of the goldfish may rely on neuromodulatory mechanisms to suppress neuronal hyper-excitability. as γ-aminobutyric acid (gaba) is the major inhibitory neurotransmitter in the brain, we decided to investigate its potential role in suppressing the electrical activity of goldfish telencephalic neurons. utilizing whole- ...	2017	27923876
ligand-biased regulation of ptdins(3,4,5)p3-dependent signal transduction in gpcr control of pituitary hormone release.	biased signaling describes the selective activation of signal transduction cascades by structurally related ligands downstream of shared g protein-coupled receptors (gpcrs). although class i phosphoinositide 3-kinases (pi3ks) are important components of gpcr-controlled transduction networks, little is known regarding the potential for biased regulation of class i pi3k-dependent signaling. the full complement of class i pi3k catalytic subunits (p110α, p110β, p110δ, and p110γ) first appears in bon ...	2017	27906555
characterization of ghrelin o-acyltransferase (goat) in goldfish (carassius auratus).	ghrelin is the only known hormone posttranslationally modified with an acylation. this modification is crucial for most of ghrelin's physiological effects and is catalyzed by the polytopic enzyme ghrelin o-acyltransferase (goat). the aim of this study was to characterize goat in a teleost model, goldfish (carassius auratus). first, the full-length cdna sequence was obtained by rt-pcr and rapid amplification of cdna ends methods. two highly homologous cdnas of 1491 and 1413 bp, respectively, name ...	2017	28178327
elevated co2 levels increase the toxicity of zno nanoparticles to goldfish (carassius auratus) in a water-sediment ecosystem.	concerns about the environmental safety of metal-based nanoparticles (mnps) in aquatic ecosystems are increasing. simultaneously, elevated atmospheric co2 levels are a serious problem worldwide, making it possible for the combined exposure of mnps and elevated co2 to the ecosystem. here we studied the toxicity of nzno to goldfish in a water-sediment ecosystem using open-top chambers flushed with ambient (400±10μl/l) or elevated (600±10μl/l) co2 for 30days. we measured the content of zn in suspen ...	2017	28040633
modulation of erythromycin-induced biochemical responses in crucian carp by ketoconazole.	the individual and combined biochemical responses of erythromycin and ketoconazole have been examined in an organism representative of the aquatic environment, crucian carp (carassius auratus). the possible interactions between erythromycin and ketoconazole were investigated on the bioaccumulation and the expression of biotransformation enzymes 7-ethoxyresorufin-o-deethylase (erod) and glutathione s-transferase (gst), and an antioxidant defense enzyme superoxide dismutase (sod) in fish tissues. ...	2017	28004371
acute exposure to the penconazole-containing fungicide topas partially augments antioxidant potential in goldfish tissues.	penconazole is a systemic fungicide commonly used in agriculture as the commercial preparation topas. although triazole fungicides are widely found in the aquatic environment, little is known about their acute toxicity on fish. in this study we assessed the effects of short-term exposure to topas on some parameters of homeostasis of reactive oxygen species (ros), such as the levels of markers of oxidative stress and parameters of the antioxidant defense system of goldfish (carassius auratus l.). ...	2017	27979696
glucose, amino acids and fatty acids directly regulate ghrelin and nucb2/nesfatin-1 in the intestine and hepatopancreas of goldfish (carassius auratus) in vitro.	ghrelin and nesfatin-1 are two peptidyl hormones primarily involved in food intake regulation. we previously reported that the amount of dietary carbohydrates, protein and lipids modulates the expression of these peptides in goldfish in vivo. in the present work, we aimed to characterize the effects of single nutrients on ghrelin and nesfatin-1 in the intestine and hepatopancreas. first, immunolocalization of ghrelin and nucb2/nesfatin-1 in goldfish hepatopancreas cells was studied by immunohist ...	2017	28089858
monitoring and assessment of heavy metal contamination in a constructed wetland in shaoguan (guangdong province, china): bioaccumulation of pb, zn, cu and cd in aquatic and terrestrial components.	the objective of this study is to evaluate the current status of heavy metal concentrations in constructed wetland, shaoguan (guangdong, china). sediments, three wetland plants (typha latifolia, phragmites australis, and cyperus malaccensis), and six freshwater fish species [carassius auratus (goldfish), cirrhinus molitorella (mud carp), ctenopharyngodon idellus (grass carp), cyprinus carpio (wild common carp), nicholsicypris normalis (mandarin fish), sarcocheilichthys kiangsiensis (minnows)] in ...	2017	27164879
an in vivo assay performed using multiple biomarkers related to testosterone synthesis and conversion for assessing the androgenic potency of refuse leachate.	refuse leachate is likely an important source of androgens. however, common in vitro bioassays underestimate the potential androgenic activity of leachate, owing to non-receptor-mediated mechanisms that modify the balance of sex hormones and promote the accumulation of endogenous androgens. this study aimed to develop an in vivo assay by using multiple biomarkers related to testosterone synthesis and conversion for assessing the potential androgenic activity of refuse leachate sampled from a mun ...	2017	27716536
relationship between oxidative stress and brain swelling in goldfish (carassius auratus) exposed to high environmental ammonia.	buildups of ammonia can cause potentially fatal brain swelling in mammals, but such swelling is reversible in the anoxia- and ammonia-tolerant goldfish (carassius auratus). we investigated brain swelling and its possible relationship to oxidative stress in the brain and liver of goldfish acutely exposed to high external ammonia (hea; 5 mmol/l nh4cl) at two different acclimation temperatures (14°c, 4°c). exposure to hea at 14°c for 72h resulted in increased internal ammonia and glutamine concentr ...	2017	27784686
identification of three selenoprotein t paralogs in goldfish (carassius auratus) and expression analysis in response to environmental stressors.	selenoproteins, which contain the selenocysteine (sec), play crucial roles in antioxidant protection. in the present study, we have identified and characterized three selenoprotein t paralogs (designated as gfselt1a, gfselt1b and gfselt2) in goldfish, carassius auratus. the secis element was found in the 3'-utrs of gfselt1a, gfselt1b and gfselt2 mrna. sequence analysis showed that they all possess the cxxu motif (where u represents sec) in the n-terminal. constitutive expressions of gfselt1a, gf ...	2017	27667707
studies on histopathological alteration of three major organs of the goldfish, carassius auratus l., of india due to myxozoan infection with special reference to scanning electron microscopic observation.	severe infections by the protozoan parasite belonging to the genera myxozoa are causing serious problems in ornamental fish reared in breeding farms. histopathological study is being used for diagnosis of the severity of diseases. myxozoan infections result in large scale histopathological damages in different fish tissues. no information is available regarding the histopathological changes of goldfish due to infection with myxozoans. the present study deals with the histopathological changes of ...	2017	27815737