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drinking induced by angiotensin ii in fishes.among 20 species of freshwater fishes examined, pseudorasbora parva, rhodeus ocellatus, cobitis anguillicaudatus, carassius auratus, oryzias latipes, gambusia affinis, and gyrinocheilus anymonieri were found to drink water like seawater fishes, while 13 remaining species did not drink. for fish species found exclusively in fresh water, angiotensin ii (aii) treatment did not induce drinking. in contrast, those freshwater fishes which survive in estuarine brackish water (leuciscus hakonensis, c. c ...19836840524
chromium and potassium accumulation influenced by body weight in goldfish (carassius auratus). 19836850119
characteristics of the hepatic monooxygenase system of the goldfish (carassius auratus) and its induction with beta-naphthoflavone.treatment of goldfish with beta-naphthoflavone (ip) resulted in induction of hepatic monooxygenase activity in a dose-dependent manner. levels of 7-ethoxyresorufin-o-deethylase and mexacarbate nadph-dependent oxidative metabolism as well as cytochrome(s) p-450 were increased. other substrates which have been associated with 3-methylcholanthrene (3-mc)-type induction in mammals were unchanged as was delta-aminolevulinic acid synthetase. enzyme activities were inhibited by carbon monoxide and pipe ...19836857672
hydrolysis of the biliary glucuronic acid conjugate of phenol by the intestinal mucus/flora of goldfish (carassius auratus).1. an investigation was carried out on the hydrolytic activity of the intestinal mucus/flora on phenylglucuronide in the bile of goldfish. 2. approximately 79% of the total phenylglucuronide (3.4 mumol) in the bile was hydrolysed after 16 h incubation with the intestinal mucus/flora. 3. of the total phenol in the aquarium water of goldfish exposed previously to phenol for 48 h, 41% was found to be phenylglucuronide when fish were placed in a phenol-free medium and were dosed hourly for 8 h with ...19836858197
immunocytochemistry of gonadotropic cells in the pituitary of some teleost species.an antiserum (anti-sgth) raised against salmon gonadotropin (sg-g100) was tested on pituitary sections of sarotherodon mossambicus, carassius auratus, anguilla anguilla (intact and estradiol-treated to induce the development of the gonadotropic (gth) cells), salmo trutta fario, and a protandric marine teleost (sarpa salpa, sparidae). using an immunoenzymologic technique, anti-sgth stained gth and thyrotropic (tsh) cells in the species studied. anti-sgth stained the granulated gth cells in s. tru ...19836862173
depression of sodium, chloride and calcium ions in the plasma of goldfish (carassius auratus) and immature freshwater- and seawater-adapted eels (anguilla anguilla l.) after acute administration of salmon calcitonin.goldfish showed a significant reduction in their plasma sodium and calcium levels 1 h after receiving 50 i.u. calcitonin per kg body weight. when 100 i.u. calcitonin were injected there was a significant fall in circulating levels of both sodium and calcium and also of chloride ions compared with those found in untreated control animals. administration of calcitonin to immature eels adapted to either freshwater or seawater conditions showed no significant change in plasma ion composition at dose ...19836875432
species differences, influence of dose and application on biotransformation of phenol in fish.1. the metabolism of phenol in goldfish (carassius auratus), rainbow trout (salmo gairdneri) and goldenorfe ide (leuciscus ideus melanotus) was compared. all three species produced phenyl sulphate, quinol sulphate and phenyl glucuronide. 2. the dose dependence of metabolism of phenol in goldfish was investigated. when the concn. of phenol was increased from 0.2 to 2 mg/l medium, that glucuronylated increased from 7 to 16% and that sulphated decreased from 63 to 47%. 3. the influence of mode of e ...19836880237
the biotransformation of three 14c-labelled phenolic compounds in twelve species of freshwater fish.1. the urinary and biliary excretion of 14c-labelled m-cresol, 1-naphthol and o-chlorophenol were investigated in 12 species of freshwater fish (bitterling, rhodeus sericeus amarus; bream, abramis brama; crucian carp, carassius carassius; goldfish, carassius auratus; gudgeon, gobio gobio; guppy, poecilia reticulata; minnow, phoximus phoximus; perch, perca fluviatilis; roach, rutilus rutilus; rudd, scardinius erythropthalmus; three-spined stickleback, gasterosteus aculeatus; tench, tinca tinca) w ...19836880238
relative in vitro effectiveness of 17 alpha, 20 beta-dihydroxy-4-pregnen-3-one and other pregnene derivatives on germinal vesicle breakdown in oocytes of ayu (plecoglossus altivelis), amago salmon (oncorhynchus rhodurus), rainbow trout (salmo gairdneri), and goldfish (carassius auratus).the relative effectiveness of several pregnene derivatives, which had previously been identified in the ovaries of ayu (plecoglossus altivelis), on germinal vesicle breakdown (gvbd) was investigated in vitro using folliculated oocytes of four species of teleosts, ayu, amago salmon (oncorhynchus rhodurus), rainbow trout (salmo gairdneri), and goldfish (carassius auratus). although some species differences existed in the relative effectiveness of each steroid on gvbd, 17 alpha, 20 beta-dihydroxy-4 ...19836884756
permanent cell lines from erythrophoromas in goldfish (carassius auratus).attempts to establish permanent cell lines from spontaneous erythrophoromas (tumors derived from red pigment cells or erythrophores) of goldfish were made with the use of biopsy specimens from 12 tumors in 10 fish. three cell lines were established that grew in vitro in synthetic medium (l-15 or dulbecco's modified eagle's minimum essential medium) supplemented with 20% fetal bovine serum for more than 8 months. one of these lines (gem-81) with high proliferative activity was cultured for over 2 ...19806928999
ultrastructural localization of delta 5-3 beta-hydroxysteroid dehydrogenase in the interrenal cells of the goldfisch (carassius auratus).the ultrastructural localization of the enzyme delta 5-3 beta-hydroxysteroid dehydrogenase (3 beta-hsd) was studied in the goldfish interrenal cells by using the potassium ferricyanide method. immersion fixation in a mixture of 1% paraformaldehyde and 0.25% glutaraldehyde results in a consistently good ultrastructural preservation and enzyme localization. a precipitate of copper ferrocyanide indicating the localization of 3 beta-hsd activity was observed in close vicinity to the smooth endoplasm ...19806933018
visualization of microtubules of cells in situ by indirect immunofluorescence.microtubule staining patterns can be visualized within cells in situ on the surface of fish scales from the squirrel fish, holocentrus ascensionis, and the common goldfish, carassius auratus, after incubation with antibodies to sea urchin tubulin and fluorescein-labeled goat antibodies to rabbit immunoglobulin g. chromatophores in situ from both species reveal a radial microtubule framework that orients the alignment of pigment granules. innervating fibers of erythrophores on the h. ascensionis ...19806935678
responses of msh and prolactin cells to 5-hydroxytryptophan (5-htp) in amphibians and teleosts.injection of 5-hydroxytryptophan (5-htp), a precursor of serotonin, induces dispersion of melanin in the amphibians, pleurodeles waltlii (urodela) and xenopus laevis (anura), in the goldfish, carassius auratus, and in the carp, cyprinus carpio. it is accompanied by a dispersion of erythrophore pigments. in the pituitaries of pleurodeles and goldfish, a stimulation of msh cells, characterized by a significant nuclear hypertrophy, is also observed; in carassius, msh cells may become degranulated. ...19806967354
forebrain connections in the goldfish support telencephalic homologies with land vertebrates.horseradish peroxidase injections into dorsomedial and dorsolateral regions of the goldfish (carassius auratus) telencephalon demonstrate, by retrograde cell labeling, that the teleost telencephalon receives a pattern of projections from the thalamus remarkably similar to those of land vertebrates. the evidence provides support for a homology between the dorsomedial region and the corpus striatum of land vertebrates and a homology between two dorsolateral regions and the dorsal and medial palliu ...19816971493
anaerobic metabolism of red skeletal muscle of goldfish. carassius auratus (l.):mitochondrial produced acetaldehyde as anaerobic electron acceptor. 19827040103
a technique for assaying the activities of alpha-gpd, ldh, and mdh in the teleost, the goldfish, carassius auratus.1. a spectrophotometric assay using rho-iodo-nitro-triphenyl tetrazolium chloride as the indicator was developed for splenic tissue in the goldfish carassius auratus. 2. this technique permits a rapid test for the activities of the enzymes lactic dehydrogenase, malic dehydrogenase, and alpha-glycerophosphate dehydrogenase which are indicative of the relative activities of various metabolic pathways.19827060353
ultrastructure of scales in a teleost (carassius auratus l.) after use of rapid freeze-fixation and freeze-substitution.new data on the ultrastructural features of the elasmoid scales of carassius auratus have been obtained by use of rapid freezing with subsequent freeze-substitution in anhydrous solvents. these are compared with the results obtained using conventional aqueous fixatives. the external layer of the scales is composed of randomly oriented collagen fibres. in the first stages of mineralization, mineral deposits are located in the interfibrillary substance where dense granules appear to be active site ...19827066978
morphology of radial glia, ependymal cells, and periventricular neurons in the optic tectum of goldfish (carassius auratus).there are three populations of cells in the deep layers of the optic tectum in a normal adult goldfish: the periventricular neurons, the ependymal cells, and the radial glia. the characteristic morphological features which distinguish the three cell populations are examined at light and electron microscopic levels in the present work. a radial glial cell has a deeply invaginated nucleus located in a subependymal layer. its cytoplasm contains mitochondria with 35-nm dark granules and 20-nm microt ...19827076888
the effects of constant light and constant darkness on the pineal organ of the goldfish, carassius auratus.goldfish (carassius auratus) were subjected to both long- and short-term (1, 3, 6, and 170 days) constant light or constant darkness to determine the effects of environmental lighting on the morphology of photoreceptor cells in the pineal organ. morphometric analysis of electron micrographs from each experimental group suggested that the effects of constant darkness are morphologically expressed only after long-term (6 months) exposure. these included an increase in the volume of photoreceptor a ...19827077259
isozyme expression in f1 hybrids between carp and goldfish.interspecific genetic differences in malate dehydrogenase (mdh), lactate dehydrogenase (ldh), superoxide dismutase (sod), and esterase (est) isozymes in carp (cyprinus carpio) and goldfish (carassius auratus) were used to examine the allelic expressions in the hybrid between these species. a unique liver sod and muscle ldh phenotype unambiguously identifies all presumed hybrid individuals. there was no evidence of f2 or backcross phenotypes in hybrid individuals. liver mdh and est phenotypes in ...19827092795
nerve growth factor stimulates neurite outgrowth from goldfish retinal explants: the influence of a prior lesion.goldfish (carassius auratus) retinal explants, whose ganglion cells were 'primed' in vivo by an optic nerve crush at varying intervals prior to culture, were found to respond by enhanced neurite outgrowth to low (ng/ml) concentrations of nerve growth factor (ngf). this in vitro response was dose-dependent and specific for ngf. the spontaneous fiber outgrowth which normally occurs in vitro in response to optic nerve lesion without exogenous ngf in the medium could be reduced by approximately 80% ...19827093729
habituation of fright and arousal responses in the teleosts carassius auratus and rutilus rutilus.the habituation of fright and arousal responses of goldfish (carassius auratus) and roach (rutilus rutilus) to the repeated operation of a plunger in the water was studied. the early response was of fright, which habituated, and the response characteristic of arousal appeared, which then habituated as well. longer intervals between stimuli required more presentations of the stimulus for habituation to occur in goldfish. roach required more presentations of the stimulus than goldfish for the resp ...19827096683
the effects of constant light and constant darkness on daily changes in the morphology of the pineal organ in the goldfish, carassius auratus.the fine structure of photoreceptor cells in the pineal organ of the goldfish was found to vary quantitatively over a 24-hour period. stereological analysis revealed significant daily changes in the volume of the cell and inner segment, nuclear volume and nucleolar diameter, volume of endoplasmic reticulum and golgi bodies, area of both rough and smooth endoplasmic reticulum, and number of vesicles associated with each golgi body. peak values of these variables occurred either during the dark ph ...19827108508
uptake and toxicity of toxaphene to cell cultures derived from goldfish (carassius auratus). 19827115996
neurohypophysial hormonal control of cortisol secretion in the teleost carassius auratus. 19827160609
structure of the mitral cell in the olfactory bulb of the goldfish (carassius auratus).the mitral cell in the olfactory bulb of the goldfish was examined by means of light microscopy, high-voltage electron microscopy, and conventional electron microscopy. mitral cells are located rather diffusely throughout the glomerular and plexiform layers. they do not make their own discrete layer. the cell bodies are rounded or triangular, and are about 10-25 micrometers in diameter. in golgi-impregnated material, thick cylindrical dendrites can be seen arising from the cell bodies and branch ...19827161415
morphologic evidence for seasonal changes in the pineal organ of the goldfish, carassius auratus; a quantitative study.a quantitative morphologic study of photoreceptor cells in pineal organ of the goldfish was conducted over a period of one year in which the photoperiod-temperature regime simulated the natural environment. statistically significant seasonal differences were found in cell size, nuclei, nucleoli, mitochondria, endoplasmic reticulum, and golgi bodies. peak values generally occurred during the fall and winter months, while minimum values coincided with the reproductive spring and summer seasons. hi ...19827163615
biochemical bases for environmental adaptation in goldfish (carassius auratus l.): resistance to ammonia.goldfish specimens were exposed for 24-48 hr to 20, 40, 600, and 2500 micrograms n/liter unionized ammonia (uia). this treatment causes, in the brain, increases in total ammonia, glutamine, lactate, and succinate and decreases in glutamate, glucidic stores, total nadh, and atp. most of these effects have already been reported in ammonia-treated trout. it is therefore suggested that the cerebral biochemical mechanisms of ammonia toxicity in these two species are fundamentally the same. the most i ...19827169039
the relationship between the activity of splenic enzymes and the immune response in the goldfish, carassius auratus. i. preliminary experiments with human a1 secretor's saliva and a1 rbc. ii. preliminary enzyme experiments using alpha-gpd and ldh.1. goldfish, carassius auratus, were injected with two antigenic substances a1 secretor's saliva or a1 rbc. 2. the goldfish produced significant antibody titers which continued for 10-12 weeks when maintained at 28 degrees c. 3. analysis of enzymatic activities of soluble and particulate alpha-gpd and ldh from skeletal muscle and spleen showed significant differences between and within tissues.19827172634
fine structural characteristics of the hepatic sinusoidal walls of the goldfish (carassius auratus).fine structural characteristics of hepatic sinusoidal walls of the goldfish were described. kupffer's stellate cells (macrophages) are few in number in the sinusoidal endothelial lining of this fish. very rarely macrophages, whose small cytoplasmic protrusion is sometimes inserted between two endothelial cells, are localized in the sinusoidal lumen in contact with the endothelial cell. typical desmosomes are frequently seen between the two endothelial cells, between the endothelial cell and the ...19807192975
timing of a single daily meal and diel variations of serum thyroxine, triiodothyronine and cortisol in goldfish carassius auratus. 19817196481
structural organization of sperm chromatin from the fish carassius auratus. 19827198994
water-electrolyte balance in goldfish carassius auratus, under constant and diurnally cycling temperature conditions.1. the effects of acclimation to constant and diurnally cycling temperatures upon water-electrolyte regulation were examined in goldfish held at 20, 25, 30 and 25 +/- 5 degrees c, and sampled at 03.00, 09.00 15.00 and 21.00 h. plasma and epaxial muscle levels of na, k, ca, mg, cl and water were determined. using cl space as an indicator of extracellular phase volume, mean cellular cation concentrations were estimated. 2. fish held at constant temperature exhibited significant diurnal variations ...19827201004
deficits in foraging behavior after telencephalon ablation in the goldfish (carassius auratus). 19817202623
deficits in habituation of cardiac arousal responses incurred by telencephalic ablation in goldfish, carassius auratus, and their relation to other telencephalic functions.the effects of telencephalic ablation on the habituation of cardiac arousal responses in goldfish was studied. the ablation was performed either by ligating the telencephalon of habituated fish between stimulus presentations or by aspiration prior to habituation. the former technique caused dishabituation; the latter, a slower rate of habituation than that of sham-operated controls. the habituation deficit incurred by surgical ablation was found to decline with time, being small after 2 wk. the ...19817251953
normal and abnormal retinal projections following the crush of one optic nerve in goldfish (carassius auratus).optic nerve regeneration was examined with [3h]proline radioautography in fish that had one nerve crushed. fibers had not yet grown beyond the crush site at 2 days post-crush (pc) and were at the optic chiasm by 4-5 days pc. by 6 days pc the fibers had reinnervated the rostral pole of the contralateral tectum, the lateral geniculate nucleus and area pretectalis dorsalis and ventralis. area preopticus, nucleus opticus dorsolateralis and nucleus opticus commissurae posterior were partially reinner ...19817263949
alkaline phosphatase and peroxidase in goldfish (carassius auratus) leukocytes.alkaline phosphatase activity and peroxidase activity were studied in granulocytes from the peripheral blood of carassius auratus by cytochemical procedures for both light and electron-microscope examination. alkaline phosphatase reaction products appeared in juvenile neutrophil granules and in portions of eosinophil granules. peroxidase reaction products were observed in mature neutrophil granules and in portions of eosinophil granules. basophils showed negative results. the presence of alkalin ...19817271703
the glomerulus of a stenohaline fresh-water teleost, carassius auratus gibelio, adapted to saline water. a scanning and transmission electron-microscopic study.prussian carp, carassius auratus gibelio, were maintained in aquaria with a salinity of 15 0/00 for 3 months. more than 90% of the glomeruli disappeared from the tissue as a result of the adaptation to saline water. kidney tissue was excised and prepared for electron microscopy after perfusion fixation in situ. the fine structure of the renal corpuscle was compared with the normal ultrastructure of glomeruli of control fish from fresh-water aquaria. the main alterations include retraction of the ...19817273133
the goldfish immune response. i. characterization of the humoral response to particulate antigens.anti-red blood cells (rbc) and anti-hapten antibody synthesis were studied in the goldfish, carassius auratus. spontaneous haemagglutination titres were found against all the antigens tested. a weak secondary response was observed in rbc-primed fish boosted during the end-phase of the primary antibody production. however, when the second antigenic challenge was performed during the early exponential phase of a primary stimulation, an important amplified response was obtained. the antibody produc ...19817275178
seasonal differences in brain activity and responsiveness shown by the goldfish, (carassius auratus). 19817283929
ruffed cell: a new type of neuron with a distinctive initial unmyelinated portion of the axon in the olfactory bulb of the goldfish (carassius auratus). iii. three-dimensional structure of the ruffed cell dendrite.the three-dimensional structure of the ruffed cell dendrite in the olfactory bulb of the goldfish (carassius auratus) was studied by means of light and high-voltage electron microscopy of golgi-impregnated material, combined golgi-electron microscopy, and electron microscopy of serial thin sections. ruffed cell dendrites ramify in the glomerular area with tufted branching patterns. they give rise to many appendages of various shapes and sizes: sheetlike processes, fingerlike projections, or smal ...19817287936
the biotransformation of [14c]phenol in some freshwater fish.1. the biotransformation of phenol was investigated in eight species of freshwater fish: bream (abramis brama), goldfish (carassius auratus), guppy (poecilia reticulata), minnow (phoxinus phoxinus), perch (perca fluviatilis), roach (rutilus rutilus), rudd (scardinius erythropthalmus) and tench (tinca tinca), when exposed to sublethal concentrations of phenol in the aquarium water. 2. the two conjugates, phenyl sulphate and phenyl glucuronide, were the sole detected products produced by bream, pe ...19817293212
some observations on perisinusoidal lipocyte (ito cell) of carassius auratus liver as revealed by electron microscopy. 19817315185
acquired immunity against trypanosoma danilewskyi in goldfish, carassius auratus.two groups of goldfish maintained at 20 degrees c were experimentally infected with two different sized inocula of trypanosoma danilewskyi. one group was inoculated with 380,000 trypanosomes/fish and the other with 3800. forty and 90% of the fish, respectively, survived. all surviving fish challenged 190 days post-infection were protected from re-infection. the plasma of these fish when inoculated into another group of fish also protected them from infection. finally, trypanosomes incubated in v ...19817329722
the effects of lithium chloride on spontaneous alternation behaviour in the goldfish (carassius auratus).spontaneous alternation behaviour of goldfish was observed in a two-unit t maze. whilst significant degrees of alternation occurred under both lithium and sodium treatment, alternation under lithium was significantly lower than under sodium. in a second experiment it was shown that lithium increased the amount of random or erratic behaviour demonstrated by fish. the effects of lithium on spontaneous alternation were fully explained by a lithium-induced increase in randomness of activity, without ...19807354909
the origins of the centrifugal fibers to the olfactory bulb in the goldfish, carassius auratus: an experimental study using the fluorescent dye primuline as a retrograde tracer.the origin of the centrifugal fibers to the olfactory bulb in the goldfish was examined by injecting a fluorescent dye, primuline, into the olfactory bulb by means of hydraulic pressure. three to five days after the primuline injection, which was confined to the unilateral olfactory bulb, a large number of primuline-labeled perikarya appeared in two telencephalic areas on the ipsilateral side: the area ventralis telencephali pars dorsalis (vd) and the ventral part of the area dorsalis telencepha ...19807357427
localization of alpha-bungarotoxin binding sites to the goldfish retinotectal projection.the optic tectum of the goldfish carassius auratus is a rich source of alpha-bungarotoxin (alpha-btx) binding protein. in order to determine whether some fraction of these receptors is present at retinotectal synapses, we have compared the histological distribution of receptors revealed by the use of [125i]alpha-btx radioautography to the distribution of optic nerve terminals revealed by the use of cobalt and horseradish peroxidase (hrp) techniques. the majority of alpha-btx binding is concentra ...19807357466
effects of pinealectomy and melatonin treatment on growth in the goldfish, carassius auratus. 19807364214
electroencephalographic studies on arousal in the goldfish (carassius auratus).goldfish with implanted intracranial electroencephalographic (eeg) electrodes showed high-voltage slow activity of 6-9 hz and a low-voltage fast activity of 16-24 hz when resting in the dark. the former frequency was prevalent in the telencephalon, diencephalon, and midbrain but remained only in parts of the midbrain and cerebellum when the fish was left resting in an illuminated environment. in this latter condition the 16-24 hz activity became predominant. presentation of two stimuli, the onse ...19807364997
periovulatory changes in serum cortisol levels in the goldfish, carassius auratus. 19807372097
carassius auratus gibelio: the prussian carp as a model for the stenohaline freshwater teleost. 19807379533
[the sequence of the alpha- and beta-chains of the goldfish hemoglobin (carassius auratus) (author's transl)].the primary structures of the alpha- and beta-chains from goldfish hemoglobin are given. the globin chains were separated by gel filtration after oxidation of globin with air. the peptides for sequencing were obtained by chemical and enzymatical cleavage. the alpha-chain has 142 residues, the beta-chain 147. the alpha-chain is acetylated at the amino-terminal residue. compared with the human chains, there are 66 amino acid differences in the alpha-chain, 72 residues are different in the beta-cha ...19807380396
physiological changes observed in the goldfish (carassius auratus) during behavioral arousal and fright. 19807387594
involvement of the preoptic region in gonadotropin release-inhibition in goldfish, carassius auratus.lesions were made in various hypothalamic and preoptic regions in sexually mature female and male goldfish. destruction of the pituitary stalk, and lateral anterior hypothalamic tract areas caused ovulation and increased serum gonadotropin levels. destruction of the entire preoptic region or a major part of the anterior nucleus preopticus periventricularis also caused ovulation and increased serum gth levels. lesions in other locations were ineffective. the results indicate that a gonadotropin r ...19807393408
behaviour of some radioactive compounds of mercury & selenium in aquarium water & their direct uptake by the goldfish carassius auratus. 19807399585
effect of sodium selenite & selenomethionine on the accumulation & acute toxicity of mercuric & methylmercuric chloride in the goldfish carassius auratus. 19807399588
retinal ganglion cell response to nerve growth factor in the regenerating and intact visual system of the goldfish (carassius auratus).light and electron microscopic observations indicated that 1 microgram ngf injected into the goldfish eye at the time of optic nerve crush initiated significant retinal ganglion cell body, nuclear and nucleolar hypertrophy when compared to controls at 7 days post-axotomy (7 dpa). in addition, the ultrastructural morphometric values for ngf-treated retinal ganglion cells at 7 dpa were not significantly different from the normal hypertrophy found in 14 dpa controls. therefore, it appears that ngf ...19807407558
continued in vitro growth of fibroblast-like cells (rbcf-1) derived from the caudal fin of the fish, carassius auratus. 19807409028
plasma clearance of gonadotropin in goldfish, carassius auratus, during the annual reproductive cycle. 19807409468
the metabolism of gonadotropin in goldfish, carassius auratus: tissue uptake and distribution during the reproductive cycle. 19807409470
time course and pattern of optic fiber regeneration following tectal lobe removal in the goldfish.following single tectal lobe removal in the adult goldfish, carassius auratus, the pattern of regeneration of the optic fibers which had previously projected to that tectum was examined at 1, 2, 4, 6, 8, 10, and 12 weeks postoperative using 3h-proline radioautography. we found that regenerating optic fibers grew across the midline through the transverse, minor, horizontal, and posterior commissures to innervate the remaining tectum. at early postoperative times innervation of the tectum was cont ...19807410595
rod and cone inputs to bipolar cells in goldfish retina.five morphological types of bipolar cells which make synaptic contact with rods and cones are distinguished in the retina of adult goldfish (carassius auratus) by characteristics readily observable in the light microscope. cells were designated type a or type b according to whether their axons terminate in the distal part (sublamina a) or proximal part (sublamina b) of the inner plexoform layer, respectively. analysis of serial semi-thin sections of golgi-impregnated cells demonstrates that each ...19807410596
histogenesis of the goldfish retina.the order of production of retinal cells was studied in embryos of the goldfish, carassius auratus, using 3h-thymidine autoradiography. cell division ceases first in the neuroepithelium at the fundus of the eye between embryonic stages 19 and 20 and gradually becomes restricted to the retinal margin by stage 24. in the fundus the cells whose nuclei will reside in the inner layer of the retina stop dividing earlier than the cells whose nuclei will reside in the outer retinal layers. thus the gang ...19807410599
apparent drug resistance to the organophosphate dimethyl (2,2,2-trichloro-1-hydroxyethyl) phosphonate by monogenetic trematodes.gyrodactylus elegans on goldfish, (carassius auratus) from a commercial farm were resistant to recommended dosages of dimethyl (2,2,2-trichloro-1-hydroxyethyl) phosphonate. controlled experiments suggest that a dosage 100 times the commonly recommended minimal dosage (.25 mg/l) was required to remove trematodes. a hypothesis is proposed to account for the development of drug resistant trematodes based on the life cycle of the parasites and continual drug exposure.19807411739
androgen biosynthesis by testes of the goldfish carassius auratus in vitro: the effect of temperature on the formation of steroid glucuronides. 19807419044
effects of salmon gonadotrophin (sg-g100) on plasma lipids in the goldfish. carassius auratus. 19807427820
effects of sex steroids on plasma lipids in the goldfish, carassius auratus. 19807427821
evidence for two secretory cell types in the stannius bodies of the teleosts fundulus heteroclitus and carassius auratus.the stannius bodies of killifish and goldfish were ultrastructurally investigated after exposure of the fish to media of different osmolality and calcium content. in both species two structurally different secretory cell types are found. type-1 cells predominate. they contain an extensive granular endoplasmic reticulum, some large golgi areas, and secretory granules with a mean diameter of about 0.4 micron. these cells are activated by transfer of freshwater fish to seawater, but not by transfer ...19807428033
the effects of lithrium on the development of alcohol tolerance in carassius auratus. 19807429745
in vivo and in vitro studies on the host specificity of trypanoplasma salmositica.the host specificity of trypanoplasma salmositica was studied by experimental inoculation into 13 species of common teleosts (notropis cornutus, notropis heterolepis, notropis spiloperus, nocomis biguttatus, rhinichthys atratulus, semotilus atromaculatus, carassius auratus, ambloplites rupestris, lepomis gibbosus, etheostoma nigrum, hypentelium nigricans, ictalurus melas, and eucalia inconstans). t. salmositica was not recovered at 14 and 40 days post inoculation. however, large numbers of the p ...19807431516
comparison of the pineal complex, retina and cerebrospinal fluid contacting neurons by immunocytochemical antirhodopsin reaction.the presence of rhodopsin was investigated by an indirect immunocytochemical method in the pineal complex of various vertebrates (carassius auratus, cyprinus carpio, hypophthalamichthys molitrix, lucioperca lucioperca, triturus vulgaris, bombina bombina, rana esculenta, pseudemys scripta elegans, lacerta agilis et viridis, white leghorn chickens, rat), in the retina of lebistes reticulatus, lucioperca lucioperca, rana esculenta, lacerta agilis, pseudemys scripta elegans, the chicken and the rat, ...19807456628
toxicity of pulsed monochloramines to goldfish (carassius auratus). 19807459458
retinal ganglion cell response to axotomy and nerve growth factor antiserum treatment in the regenerating visual system of the goldfish (carassius auratus): an in vivo and in vitro analysis.in vitro nerve growth factor (ngf) antiserum (anti-ngf) treatment was found to significantly depress retinal ganglion cell neurite outgrowth in goldfish explant culture. goldfish retinas, conditioned by a 14-day prior optic nerve crush, demonstrated a significant dose response inhibition of neurite outgrowth if incubated with various concentrations of the antiserum (i.e. concentrations from full strength to 1:100) before explantation for tissue culture. ngf added to the incubation medium contain ...19817459632
brain lesions and short-term endocrine effects of monosodium l-glutamate in goldfish, carassius auratus.monosodium l-glutamate (msg) was injected intraperitoneally into goldfish at a dosage of 2.5 mg/g body weight. at 24 h post-injection there was a marked hypertrophy and edema in the region of the nucleus lateralis tuberis (nlt) from the anterior margin of the pituitary stalk through to the posterior end of the nlt, irrespective of the sex of the goldfish. a similar hypertrophy and edema occurred ventral to the anterior commissure in the preoptic region in the anterior-ventral nucleus preopticus ...19807459988
photoperiod effects on carbohydrate metabolites in the goldfish, carassius auratus: role of the pineal and retinal pathways.the effects of exposure to various photoregimes or to constant darkness on liver glycogen and plasma glucose levels in the goldfish were examined during different seasons. in addition, pinealectomized fish and optic nerve sectioned fish were exposed to the photoregimes to assess the potential role of the pineal organ and retinal pathways in mediating photoperiod effects. neither photoperiod manipulation nor exposure to constant darkness affected liver glycogen levels in experiments conducted dur ...19807465877
reinforcing properties of the neuropeptide substance p in carassius auratus: evidence of dopaminergic system involvement.the aim of the present study was to investigate whether neuropeptide substance p (sp) has reinforcing effects in carassius auratus and whether this effect could be related to dopaminergic neurotransmission. for this purpose fishes were put in a three-compartment box in which one compartment gave access to two others that did not directly link. the time spent in each compartment was registered for 10 min to determine a possible preferred compartment. twenty-four hours later, the fish were given o ...19957535468
substance p-, f8famide-, and a18famide-like immunoreactivity in the nervus terminalis and retina of the goldfish carassius auratus.we re-investigated the occurrence of substance p-like immunoreactivity in the retina of the goldfish carassius auratus using antisera to substance p and other tachykinins. most antisera labelled a previously described single class of mono-stratified amacrine cells arborizing in layer 3 of the inner plexiform layer. preabsorption experiments showed that these amacrine cells contained at least one tachykinin-like peptide. one antiserum (inc 353) to substance p labelled not only these amacrine cell ...19957541719
diffusion coefficients of atp and creatine phosphate in isolated muscle: pulsed gradient 31p nmr of small biological samples.measurements of the intracellular diffusion coefficients (di) of atp and creatine phosphate (pcr) in stable, isolated preparations of skeletal muscle were made by means of pulsed field gradient (pfg) 31p nmr. experiments used a pfg nmr probe specifically designed for small, superfused biological samples. this provided a magnetic field gradient in the z axis of up to 195 g/cm with minimal eddy currents. diatp and dipcr in white (fast, glycolytic) skeletal muscle from goldfish (carassius auratus) ...19957547189
histochemical and immunocytochemical localization of nitric oxide synthase in the central nervous system of the goldfish, carassius auratus.the distribution of the neuronal type of nitric oxide synthase in the goldfish brain and spinal cord was investigated via nadph-diaphorase histochemistry and immunocytochemistry using an antiserum raised against the purified mammalian enzyme. many structures, including magnocellular neurosecretory cells, motoneurons, mesencephalic trigeminal neurons, and radial glial fibers, were stained by the nadph-diaphorase reaction but were not immunoreactive. this nonspecific nadph-diaphorase activity was ...19957560292
[changes in the nucleolar characteristics in fish embryo cells under the action of low doses of radiation].the nucleolus activity of fish embryo cells was stimulated by low-level radiation at a dose rate of 2-13 mgy/h. the size of nucleoli generally increased in embryos of cyprinus carpio, whereas the number of nucleoli was greater in embryos of carassius auratus gibelio. the higher the functional activity of nucleolus is, the more pronounced are changes in the characteristics. the size of single nucleolus at gastrulation is the most sensitive characteristic.20167581803
effect of mptp on dopaminergic neurons in the goldfish brain: a light and electron microscope study.the neurotoxin mptp (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) causes a parkinsonian syndrome in the goldfish (carassius auratus), characterized by transient bradykinesia, the accumulation of mpp+ in the brain, and a decrease in the forebrain and midbrain content of catecholamines (pollard et al., faseb j., 6 (1992) 3108-3116). using light and electron microscopy, we studied the effect of mptp on the distribution and ultrastructure of tyrosine hydroxylase (th)-immunoreactive, dopaminergic ne ...19957583312
specific [3h]spiperone binding sites in the pituitary of rainbow trout (oncorhynchus mykiss) and goldfish (carassius auratus).dopamine, a catecholamine neurohormone, modulates pituitary hormone release in teleost fishes and other vertebrates. the existence and binding parameters of a pituitary dopamine-neuroleptic receptor from trout were examined and compared with those from goldfish. pituitary homogenate was incubated with [3h]spiperone (d2 antagonist) under several experimental paradigms; incubations were terminated by filtration and bound 3h radioactivity was assessed by liquid scintillation spectroscopy. specific ...19957585324
the warm temperature acclimation-related 65-kda protein, wap65, in goldfish and its gene expression.cdnas encoding a warm temperature acclimation-related protein (wap65) were cloned from the muscle and hepatopancreas cdna libraries of the warm temperature-acclimated goldfish carassius auratus, and their nucleotide sequences containing 5'- and 3'-noncoding regions together with their polyadenylation signal were determined. the deduced amino acid sequence of wap65 was 31% homologous to rat hemopexin. however, goldfish wap65 lacked a few possible glycosylation sites and presumed functional histid ...19957615502
invertebrate and vertebrate immune cells express pro-opiomelanocortin (pomc) mrna.we show here that hemocytes and leukocytes with phagocytic activity from both invertebrates (planorbarius corneus, viviparus ater) and vertebrates (carassius auratus, rana esculenta) express pro-opiomelanocortin (pomc) mrna, as assessed by in situ hybridization with a digoxigenin-labeled human dna probe. these data are in accord with previous observations from our laboratories on the presence in these cells of pomc-derived peptides and strongly suggest that these molecules--highly conserved thro ...19957620207
distribution of neurons projecting to the trochlear nucleus in goldfish (carassius auratus).the distribution of neurons projecting to the trochlear nucleus of goldfish (carassius auratus) was studied by the electrophoretic injection of horseradish peroxidase into the nucleus. the location of the injection site was electrophysiologically determined by the antidromic field potential elicited from the trochlear nucleus after the electrical stimulation of its nerve. retrogradely labeled neurons were observed in the following structures: (1) anterior, tangential and descending nuclei of the ...19957620876
thyrotropin-releasing hormone receptors in the pituitary of rainbow trout (oncorhynchus mykiss).binding parameters of radiolabeled pglu-3-me-his2-pro-nh2 ([3h]metrh) to pituitary trh receptors of rainbow trout (oncorhynchus mykiss) and goldfish (carassius auratus) were investigated. washed pituitary membranes were incubated with [3h]metrh in the absence (b0) or presence of trh or trh analogs under various paradigms. specific binding (bsp) was thermolabile and tissue dependent. association of bsp was slow (k + 1 = 1.43 x 10(7) m-1 min-1) achieving equilibrium binding after 60 min, and remai ...19957622016
immunocytochemical localization of sgnrh and cgnrh-ii in the brain of goldfish, carassius auratus.the immunocytochemical distribution of salmon gonadotropin-releasing hormone (sgnrh) and chicken gnrh-ii (cgnrh-ii) neurons in the brain of goldfish was examined using respective antisera. salmon gnrh-immunoreactive (ir) cell bodies were localized in the area between the olfactory nerve and the olfactory bulb (the terminal nerve ganglion), the ventral telencephalon, the preoptic area, and the hypothalamus. chicken gnrh-ii-ir cell bodies were observed in the same areas as were those of sgnrh, alt ...19957629310
effects of season, temperature, and photoperiod on plasma melatonin rhythms in the goldfish, carassius auratus.effects of season, environmental temperature, and photoperiod on plasma melatonin concentrations were studied in the goldfish, carassius auratus. when goldfish were reared under natural conditions, melatonin levels at mid-dark exhibited seasonal changes, with higher levels obtained in june and september than in december and march. when fish were kept under light:dark (ld) cycle of 12:12 at 5, 15, or 25 degrees c during march-april, temperature-dependent increases in melatonin levels at mid-dark ...19957629692
biochemical and pharmacological evidence for the presence of a1 but not a2a adenosine receptors in the brain of the low vertebrate teleost carassius auratus (goldfish).in whole brain membranes of goldfish, 3h-chlorocyclopentyladenosine bound to adenosine a1 receptors. the a1 receptors were ubiquitously distributed in the brain with a maximum in the hypothalamus and a minimum in the spinal cord. in superfused goldfish cerebellar slices, cyclohexyladenosine inhibited the cyclic amp accumulation stimulated by forskolin and the selective adenosine a1 receptor antagonist, 8-cyclopentyltheophylline, reversed this effect. in the same brain preparation, 30 mm k+ stimu ...19957633334
development of new nephrons in adult kidneys following gentamicin-induced nephrotoxicity.gentamicin-induced nephrotoxicity results in necrosis of proximal tubular epithelial cells. in mammals, the injured nephron can be repaired by migration and division of surviving cells. we examined this repair process in the fresh-water goldfish, carassius auratus. fish were given a single intraperitoneal injection of 50 mg/kg gentamicin and sacrificed at 1, 4, 14, and 21 days. injured nephrons showed regeneration along the basement membrane several days following gentamicin administration. in a ...19957644760
characterization of basic proteins from goldfish myelin.myelin basic protein (mbp) from common goldfish (carassius auratus) myelin was extracted with dilute mineral acid. immunological cross-reactivity of the goldfish mbp, with polyclonal antisera raised against bovine mbp, suggested that the goldfish protein has epitopes for these antibodies. it also reacted with a monoclonal antibody specific for a seven amino acid epitope (130-137) conserved in the mbp of most mammalian species. to characterize the charge heterogeneity of this protein, we iodinate ...19937678038
involvement of protein kinase c in agonist-stimulated goldfish ovulation.the effects of two protein kinase c (pkc) inhibitors, calphostin c and staurosporine, on the in vitro ovulation of goldfish (carassius auratus) oocytes were investigated. ovulation was stimulated by prostaglandin (pg) f2 alpha (pgf2 alpha, 2.0 micrograms/ml), by sodium orthovanadate (0.1 mm), by a combination of the phorbol ester phorbol 12-myristate-13-acetate (pma, 0.1 micrograms/ml) and calcium ionophore a23187 (0.05 micrograms/ml), by thapsigargin (0.2 micrograms/ml), and by elevated ph (8.1 ...19937683501
topographic organization of descending projection neurons to the spinal cord of the goldfish, carassius auratus.several neurons from different nuclei give rise to descending spinal tracts and project to various levels in the spinal cord of goldfish, carassius auratus. these were visualized by retrograde transport of horseradish peroxidase (hrp) administered to the bilaterally transected spinal cord at 6 levels, corresponding to 1st, 5th, 10th, 15th, 20th and 25th segments of the vertebral column. as many as 16 brain nuclei or neuronal aggregations and the mauthner cells project posteriorly up to the 20th ...19937690299
melatonin signal transduction in the goldfish, carassius auratus.generation and reception of melatonin signals in the goldfish, carassius auratus, are reviewed. the photoreceptive pineal gland of the goldfish generates circulating melatonin rhythms according to a given photoperiod under light-dark cycles and in a circadian manner under continuous dark conditions. melatonin is also produced in the retina in a similar fashion. melatonin produced in the pineal gland and retina is considered to act as internal zeitgeber in the brain and retina, respectively, cont ...19947708370
expression of the antifreeze protein gene in transgenic goldfish (carassius auratus) and its implication in cold adaptation.ocean pout (macrozoarces americanus) antifreeze protein (afp) genes (approximately 10(6) copies) were microinjected into the oocytes of goldfish (carassius auratus). out of a total of 303 oocytes injected, 235 (77.6%) were normally fertilized after in vitro maturation and insemination, and 136 (57.9%) of the fertilized eggs were hatched. dot blot analysis of genomic dna from 2-month-old fish indicated that 30 of the fish (22%) were positive for the afp transgene. the numbers of integrated afp ge ...19957749462
comparative anterograde amnestic and anticonvulsant effects of two types of nmda receptor antagonists: mk-801 and ha-966.the anterograde amnestic effects of non-competitive nmda antagonists mk-801 and ha-966 on classic fear conditioning in goldfish (carassius auratus) were examined in a series of experiments. experiments 1 and 2 contrasted the anterograde amnestic effects of mk-801, (+)ha-966, and (-)ha-966. experiment 3 examined the effects of mk-801 and (+)ha-966 on the expression of conditioned responses. experiments 4 and 5 investigated whether the potency of mk-801, (+)ha-966 or (-)ha-966 in blocking nmda-ind ...19957770609
myxobolus cultus n. sp. (myxosporea: myxobolidae) in the goldfish carassius auratus transformed from the actinosporean stage in the oligochaete branchiura sowerbyi.a new myxosporean myxobolus cultus n. sp. was found in experimentally infected goldfish carassius auratus exposed to actinosporean spores from the oligochaete branchiura sowerbyi (tubificidae). at 2-4 mo following initial exposure, spores of m. cultus were observed in the cartilage of goldfish. a lymphocytic infiltrate surrounded the pseudocysts. some pseudocysts were destroyed and spores had been engulfed by phagocytes. phagocytized spores were also found in the epidermis of skin and within mel ...19957776133
acoustic response properties of single neurons in the central posterior nucleus of the thalamus of the goldfish, carassius auratus.acoustic responses were recorded extracellularly from single neurons in the thalamic central posterior nucleus (cp). spontaneous activity, best sensitivity, and sharpness of tuning (q10db) of cp neurons ranged from 0 to 36 spikes/s, -40 to 5 db re: 1 dyne/cm2, and 0.18 to 1.80, respectively. the distribution of characteristic frequency (cf) was nonuniform with a mode at 195 hz. temporal response patterns of cp neurons (n = 60) were categorized into three groups: phasic (25%), tonic chopper-like ...19957776269
receptor binding of gonadotropin-releasing hormone antagonists that inhibit release of gonadotropin-ii and growth hormone in goldfish, carassius auratus.in goldfish, gnrh stimulates gonadotropin-ii (gth-ii) and growth hormone (gh) release. the two native forms of gnrh, salmon gnrh (sgnrh) and chicken gnrh-ii (cgnrh-ii), bind to two classes of gnrh binding sites: high-affinity/low-capacity sites and low-affinity/high-capacity sites. our previous in vitro perifusion studies of goldfish pituitary fragments showed that [ac-delta 3-pro1, 4fd-phe2, d-trp3,6]-mgnrh (analog e), [ac-delta 3-pro1, 4fd-phe2, d-trp3,6]-sgnrh (analog c), and [ac-d(2)nal1, 4c ...19947803606
a new class of dna photolyases present in various organisms including aplacental mammals.dna photolyase specifically repairs uv light-induced cyclobutane-type pyrimidine dimers in dna through a light-dependent reaction mechanism. we have obtained photolyase genes from drosophila melanogaster (fruit fly), oryzias latipes (killifish) and the marsupial potorous tridactylis (rat kangaroo), the first photolyase gene cloned from a mammalian species. the deduced amino acid sequences of these higher eukaryote genes show only limited homology with microbial photolyase genes. together with th ...19947813451
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