Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| two pathways for choline transport in eel erythrocytes: a saturable carrier and a volume-activated channel. | choline transport in eel (anguilla anguilla) erythrocytes was investigated in cells suspended in isotonic and hypotonic media. in cells in isosmotic solution choline transport was mediated by a saturable system with a michaelis constant (km; 62 +/- 6 microm) similar to that of the choline carrier of human erythrocytes but a maximal transport rate (vmax; 4.5 +/- 0.4 mmol.1 red blood cells-1.h-1) almost two orders of magnitude higher than that in human red blood cells. this pathway was inhibited b ... | 1994 | 8092322 |
| determination of the ca2+ and mg2+ affinity constants of troponin c from eel skeletal muscle and positioning of the single tryptophan in the primary structure. | the complete amino acid sequence of troponin c (etnc) from the white muscle of the european eel has been determined by edman degradation procedures. its single tryptophan residue is situated in helix h at amino acid position 152 of the aligned sequence; the tryptophan is the first residue on the c-terminal side of ca2+ binding loop iv. the increase of tryptophan fluorescence emission intensity occurring upon titration of etnc with ca2+ has been used to determine the affinity constants of etnc fo ... | 1993 | 8126218 |
| angiotensin ii receptor subtypes in eel (anguilla anguilla). | previous studies have shown the effects of angiotensin ii (ang ii) in teleosts, and ang ii-binding sites have also been localized in tissues from rainbow trout. the purpose of this study was to extend these findings and to provide an analysis of ang ii receptor (ang ii-r) isoforms in three tissues obtained from european eel (anguilla anguilla). ang ii-rs were identified in eel liver, kidney and intestine membranes by the binding of either 0.5 nmol human 125i-labelled tyr4-ile5-ang ii/l or increa ... | 1994 | 8185815 |
| the distribution of gaba-immunoreactive neurons in the brain of the silver eel (anguilla anguilla l.). | the distribution of gaba-immunoreactivity was studied in the brain of the silver eel (anguilla anguilla) by means of antibodies directed against gaba. immunoreactive neuronal somata were distributed throughout the brain. positive perikarya were detected in the internal cellular layer of the olfactory bulb, and in all divisions of the telencephalon, the highest density being observed along the midline. numerous gaba-reactive cell bodies were found in the diencephalon, particularly in the preoptic ... | 1994 | 8192235 |
| synthesis of vitellogenin by eel (anguilla anguilla l.) hepatocytes in primary culture: requirement of 17 beta-estradiol-priming. | control and 17 beta-estradiol-primed eels were used to investigate the hormonal requirement of vitellogenesis in an immature fish, the eel. a primary culture of isolated liver cells from female silver eels was developed. the hepatocytes were maintained as monolayers on poly-l-lysine coated dishes for up to 12 days in a defined medium alone or supplemented with 17 beta-estradiol (e2, from 10(-8) to 10(-5) m). the amounts of vitellogenin (vg) in the cells and secreted into the medium were measured ... | 1993 | 8224775 |
| effect of lipid content of diet on cholesterol content and cholesterogenic enzymes of european eel liver. | the effect of dietary lipid levels on the levels of cholesterol and the activities of the major cholesterogenic enzymes of the liver has been studied in the european eel. an increase in hepatic total cholesterol was observed when the dietary lipid levels increased from 12 to 20%, while protein levels were maintained at 30%. this change paralleled an increase in mevalonate 5-pyrophosphate decarboxylase activity, while 3-hydroxy-3-methylglutaryl-coa reductase mevalonate kinase and mevalonate 5-pho ... | 1993 | 8246691 |
| the branching of insulin-like growth factor 1 and insulin: an immunohistochemical analysis during phylogeny. | the co-existence of insulin-like growth factor 1 (igf-1) with the classical islet hormones insulin (ins), glucagon (gluc), somatostatin (som) and pancreatic polypeptide (pp) in the endocrine pancreas of representative species of cyclostomes (myxine glutinosa), cartilaginous fish (raja clavata, squalus acanthias) and bony fish (cottus scorpius, carassius auratus, cyprinus carpio, anguilla anguilla) was studied by the use of monoclonal and polyclonal antisera and the double immunofluorescence tech ... | 1993 | 8265818 |
| ascorbic acid transport by intestinal brush-border membrane vesicles of the teleost anguilla anguilla. | transport of l-ascorbate by intestinal brush-border membrane vesicles of european eel anguilla anguilla was stimulated by a transmembrane na gradient (out > in) but not by a similarly directed gradient of k. under short-circuited membrane potential conditions, a kinetic analysis of l-ascorbate influx indicated the presence of a single na-dependent carrier process (kapp = 0.75 +/- 0.07 mm and jmax = 0.33 +/- 0.03 nmol.mg protein-1.min-1) and a nonsaturable transfer component with an apparent diff ... | 1993 | 8322981 |
| peptide hormone-stimulated second messenger production in the teleostean nephron. | renal actions of arginine vasotocin (avt) and atrial natriuretic peptide (anp) in teleosts are poorly defined. administration of these hormones changes renal function, largely explained by systemic vascular effects. the present experiments examine the potential direct action of these hormones on the teleost nephron. this has been assessed from the hormones' abilities to stimulate their second messenger systems within isolated nephroi of the rainbow trout onchorhynchus mykiss. following initial e ... | 1993 | 8392960 |
| induction of g-bands on anguilla anguilla chromosomes by the restriction endonucleases haeiii, hinfi, and msei. | fixed metaphase chromosomes of the eel anguilla anguilla were treated with the restriction enzymes haeiii, hinfi, and msei. all three restriction endonucleases are capable of inducing a g-band-like pattern if the incubation time and enzyme concentration are controlled. the results are discussed in relation to the main factors involved in the appearance of serial g-bands. chromatin organization, rather than dna composition, seems to account for the interstitial g-band pattern exhibited by these e ... | 1994 | 8404070 |
| effect of hco-3 and ph on ion transport in the posterior intestine of the freshwater-adapted teleost anguilla anguilla. | the intestinal mucosa may be exposed to acidic or alcaline solutions due to liberation of digestive secretions. in several situations blood ph may also change. consequently, the effects of hco-3, co2, and ph variation of medium on the ion transport across the posterior intestine of the eel (anguilla anguilla) adapted to freshwater were studied in terms of fractional values of short-circuit current (scc), transepithelial potential difference (tpd) and conductance (g). immature eels weighing 100-2 ... | 1995 | 8520526 |
| pesticide effects on eel metabolism pesticide; levels did not decline at any time when animals were exposed to 4.1 mg/liter. | previous works on endosulfan eel toxicology in this laboratory demonstrated that 0.041 mg/liter of endosulfan was the 50% lethal concentration of 96 hr exposure. eels of species anguilla anguilla were exposed to two sublethal endosulfan concentrations: 8.2 micrograms/liter (1/5 lc50) and 4.1 micrograms/liter (1/10 lc50), and the experiment was done at different exposure times: 12, 24, 48, 72, and 96 hr. muscle glycogen content decreased significantly (p < 0.05) at 24, 48, 72, and 96 hr exposure ... | 1995 | 8521781 |
| influence of flumequine on in vivo mitogen responses of european eel (anguilla anguilla l., 1758) lymphoid cells. | the influence of flumequine on mitogen induced lymphoid cell proliferation in european eels (anguilla anguilla l., 1758) was studied. for this purpose an in vivo test, using peroral drug administration followed by successive intraperitoneal injections with concanavalin a (cona) or bacterial lipopolysaccharides (lps) and 5-bromo-2'-deoxyuridine, was applied. direct counting of proliferated cells in blood smears revealed that flumequine possesses mitogenic properties. a synergistic and an antagoni ... | 1995 | 8533292 |
| primary sequence, tissue specificity and expression of the na+,k(+)-atpase alpha 1 subunit in the european eel (anguilla anguilla). | the entire cdna nucleotide sequence of the na+,k(+)-atpase alpha 1 isoform was cloned from the gills of the european eel (anguilla anguilla) by a pcr based method. the amino acid sequence translated from the sequence shared 89.4 and 85.6% homology respectively with previously published na+,k(+)-atpase alpha subunit sequences from elasmobranch (torpedo californica) and teleost (catostomus commersoni) fish. the size of na+,k(+)-atpase alpha 1 mrna transcripts in eel tissues was demonstrated to be ... | 1995 | 8574922 |
| the role of the renin-angiotensin system in the control of blood pressure and drinking in the european eel, anguilla anguilla. | the role of the renin-angiotensin system (ras) in the control of blood pressure and drinking was investigated in fresh water (fw)- and seawater (sw)-adapted eels, anguilla anguilla, by comparing the effects of pharmacological manipulation through the use of papaverine (stimulator) and captopril (inhibitor) on the endogenous system. in sw eels basal blood pressure levels were lower (23.3 +/- 0.8 mm hg) with correspondingly higher basal drinking rates (0.51 +/- 0.07 ml/kg/hr) and plasma aii concen ... | 1995 | 8575657 |
| the molecular basis for the green-blue sensitivity shift in the rod visual pigments of the european eel. | when the european eel matures sexually and migrates back to deep sea breeding grounds the visual pigments in its rod photoreceptors change from being maximally sensitive to green light to being maximally sensitive to blue light. in part, this change in sensitivity is due to a change in the opsin component of the visual pigment molecule. we used hormone injection to induce these developmental changes in a group of eels and from these animals an opsin coding region was cloned and sequenced using c ... | 1995 | 8587887 |
| different effects of cgmp and camp in the intestine of the european eel, anguilla anguilla. | the regulation of salt absorption in the sea water eel intestine was studied by evaluating the effects of theophylline, 8 br cyclic adenosine monophosphate, 8 br cyclic guanosine monophosphate, atriopeptin iii, porcine vasoactive intestinal peptide and prostaglandin e1 on the short-circuit current, the transepithelial voltage difference and conductance and on the dilution potentials. it was shown that theophylline increased the transepithelial conductance and reduced the magnitude of the dilutio ... | 1996 | 8621839 |
| chromosomal localization of the major and 5s rrna genes in the european eel (anguilla anguilla). | the major (18s, 5.8s, and 28s) and 5s rrna genes have been mapped by double fluorescent in situ hybridization to european eel metaphase chromosomes. the major rrna genes were localized to a submetacentric pair of chromosomes that showed a consistent size polymorphism among the individuals studied. the 5s rrna genes were clustered in a single locus that mapped to the centromeric region of an acrocentric pair. in contrast to the major rrna genes, no detectable polymorphism, in either size or inten ... | 1996 | 8646885 |
| fluorescence-labeled fucolectins are superior markers for flow cytometric quantitation of the human sperm acrosome reaction. | to evaluate the binding of three fluorescein (fitc)-labeled fucose-specific lectins, anguilla anguilla agglutinin (aaa), tetragonolobus purpureas agglutinin (tpa), and ulex europaeus-1 agglutinin (uea-1), to unfixed, acrosome-intact and acrosome-reacted (ar) human sperm by flow cytometry and to compare the results with those found using fitc-labeled pisum sativum agglutinin (psa) and arachis hypogaea agglutinin (pna). | 1996 | 8654649 |
| a monoclonal antibody to mammalian angiotensin ii at1 receptor recognizes one of the angiotensin ii receptor isoforms expressed by the eel (anguilla anguilla). | using labelled ligand-binding methods, previous studies have identified specific angiotensin ii receptors (ang ii-rs) in eel liver, kidney and intestine membranes. isoelectric focusing on polyacrylamide gels also showed that there are two ang ii-r isoforms in eel liver, focusing at isoelectric points (pi) 6.5 and 6.7. these may have different functions. in contrast, eel enterocyte plasma membrane and renal brush border membranes contain only the pi 6.5 form. to characterize the eel receptors mor ... | 1996 | 8672232 |
| angiotensin ii stimulation of the basolateral located na+/h+ antiporter in eel (anguilla anguilla) enterocytes. | the ph-sensitive fluorescent dye, 2',7'-bis-carboxyethyl-5, 6-carboxyfluorescein acetoxymethyl ester, was used to examine the effects of fish or human angiotensin ii (ang ii) on the activity of the basolateral located na+/h+ antiporter in eel intestinal cell suspensions. exposure of eel enterocytes to either hormone led to an increased activity of the antiporter. this time- and dose-dependent stimulatory effect was inhibited by the specific antiporter inhibitor dimethylamiloride (dma). preincuba ... | 1996 | 8672233 |
| purification and characterization of the tricarboxylate carrier from eel liver mitochondria. | the tricarboxylate carrier from eel (anguilla anguilla) liver mitochondria was solubilized with triton x-100 and purified by sequential chromatography on hydroxyapatite and matrex gel blue b. on sds-polyacrylamide gel electrophoresis, the purified fraction showed a single polypeptide band with an apparent molecular mass of 30.4 kda. when reconstituted into liposomes, the tricarboxylate transport protein catalyzed a very active 1,2,3-benzenetricarboxylate-sensitive citrate/citrate exchange. it wa ... | 1996 | 8687426 |
| bicarbonate absorption in eel intestine: evidence for the presence of membrane-bound carbonic anhydrase on the brush border membranes of the enterocyte. | bicarbonate absorptive fluxes through the isolated intestine of the european eel (anguilla anguilla) were evaluated by the ph-stat method under short-circuited conditions. it was found that bicarbonate absorptive flux was dependent on the luminal na+ and was inhibited by luminal 4-acetamido-4' stilbene-2-2' disulfonic acid (sits; 2.5 x 10(-4) m) and luminal acetazolamide (10(-4) m), while luminal amiloride (1 mm) was without effect. furthermore, by using brush border membrane vesicles (bbmv) iso ... | 1996 | 8691189 |
| absorbance spectra and molecular structure of the blue-sensitive rod visual pigment in the conger eel (conger conger). | the conger eel (conger conger) is a nocturnal fish that can be found living in shallow coastal water and deep water down to 1000 m. the conger eel has a pure rod retina with a visual pigment maximally sensitive to blue light around 487 nm. we have cloned and sequenced the opsin cdna which is presumed to code for this visual pigment and have found it to be highly homologous to the form of opsin that is expressed in mature, deep-living european eels (anguilla anguilla). the opsin sequence informat ... | 1996 | 8763796 |
| effects of serotonin on the cardio-circulatory system of the european eel (anguilla anguilla) in vivo. | the effects of serotonin on continuously recorded cardiac parameters (heart rate, cardiac output, cardiac stroke volume), ventral and dorsal aortic blood pressures, branchial and systemic vascular resistances were investigated in the european eel in vivo. intravenous administration of serotonin (30 micrograms.kg-1) caused a marked bradycardia (45%) and a simultaneous decrease in cardiac output (50%), ventral (35%) and dorsal (50%) aortic blood pressures. branchial resistance was markedly increas ... | 1996 | 8766910 |
| swelling-activated k+ transport via two functionally distinct pathways in eel erythrocytes. | following osmotic swelling, erythrocytes from the european eel, anguilla anguilla, underwent a regulatory volume decrease. this was prevented by replacement of na+ with k+ in the suspending medium, consistent with a role for the (normally outward) electrochemical k+ gradient in the volume-regulatory response. the effect of cell swelling on k- transport in these cells was investigated using 86rb+ as a tracer for k+. osmotic swelling resulted in an increase in ouabain-insensitive k+ transport that ... | 1996 | 8769785 |
| genomic variation of aquatic birnaviruses analyzed with restriction fragment length polymorphisms. | aquatic birnaviruses are the most ubiquitous and diverse group of viruses in the family birnaviridae. several cause different diseases in a variety of fish species, such as infectious pancreatic necrosis virus in salmonids in north america, europe, and asia and european eel virus in eel in asia. most isolates are antigenically related and belong to a single serogroup (serogroup a) comprising nine serotypes. previous studies with monoclonal antibodies have demonstrated considerable variation in e ... | 1996 | 8779591 |
| control of acid secretion in cultured gas gland cells of the european eel anguilla anguilla. | single cells and cell clusters isolated from the swimbladder epithelium of the european eel anguilla anguilla attached to collagen s-coated petri dishes and proliferated in a modified dulbecco's modified eagle's medium, supplemented with 0.5% fetal calf serum. at a temperature of 20-22 degrees c, the growing colonies reached confluence typically within 6-8 days. activities of glycolytic and pentose phosphate shunt enzymes remained stable or increased only slightly during the first 10 days of pri ... | 1996 | 8780223 |
| development of a radioimmunoassay for european eel growth hormone and application to the study of silvering and experimental fasting. | a specific and sensitive homologous radioimmunoassay for eel (anguilla anguilla l.) growth hormone (anggh) has been developed. the antiserum, raised against purified anggh and used at 1:20,000 final dilution, did not cross-react with eel prolactin or thyrotropin, carp gonadotropin ii, bovine gh, or serum from hypophysectornized eel. the inhibition curves for eel pituitary extracts and serum were parallel to that of anggh standard. the ed50 value was between 1 and 2 ng/tube and the recovery of pu ... | 1996 | 8804566 |
| influence of flumequine and oxytetracycline on the resistance of the european eel against the parasitic swimbladder nematode anguillicola crassus. | the effect of the antibacterial drugs flumequine (fq) and oxytetracycline (otc) on the defence system of the european eel (anguilla anguilla l., 1758) was investigated using an experimentally induced infection with the parasitic swimbladder nematode anguillicola crassus. eight weeks after oral administration of infective larvae, the mean recovery of the parasites in fq-treated eels was lower than in non-medicated control animals, and significantly lower than in otc-treated eels. mean numbers of ... | 1996 | 8807782 |
| changes in enzyme histochemical profiles of identified spinal motoneurons of the european eel, anguilla anguilla, following cordotomy. | the enzyme histochemical profiles of glucose-6-phosphate dehydrogenase (a marker of synthetic performance), succinate dehydrogenase (an indicator of oxidative metabolism), and nadh tetrazolium reductase (a marker of overall neuronal activity) were determined for identified white muscle motoneurons in six control and six cordotomized cels. images were digitized and mean integrated absorbances obtained using appropriate hardware and software. for motoneurons caudal to the transection site there wa ... | 1996 | 8818680 |
| genome size and at content in anguilliformes. | the genome sizes (gs) and at contents of five species of anguilliformes (anguilla anguilla, anguilla rostrata, conger conger, gymnothorax unicolor and muraena helena) belonging to three families (anguillidae, congridae and muraenidae) were determined by flow cytometry and densitometry. differences in gs between anguillidae-congridae (about 3 pg) and muraenidae (about 5 pg) were found, which correlate with the relative amounts of at base pairs (around 42-43% for anguillidae-congridae and 51-52% f ... | 1995 | 8835179 |
| physiological stress responses of anguilla anguilla to fenitrothion. | the present communication deals with the effect of fenitrothion (0.04 ppm) on the carbohydrate metabolism of the european eel anguilla anguilla and its recovery from intoxication. analysis of various parameters such as glycogen, lactate and glucose was made on different eel tissues after 2, 8, 12, 24, 32, 48, 56, 72 and 96 hr of fenitrothion exposure. subsequently, the fish were allowed recovery periods of 8, 12, 24, 48, 72, 96, 144 and 192 hr in clean water, and the same parameters were evaluat ... | 1996 | 8847448 |
| changes in gas composition in the swimbladder of the european eel (anguilla anguilla) infected with anguillicola crassus (nematoda). | the effect of anguillicola crassus (nematoda) on gas composition in the swimbladder of eels (anguilla anguilla) was studied using mass spectrometry in feral eels from two german rivers and in experimentally infected eels. in both naturally and experimentally infected eels significant correlations were observed between the proportion of oxygen in the swimbladder and level of infection with anguillicola crassus. in swimbladders of naturally infected feral eels the contribution of oxygen to swimbla ... | 1996 | 8851864 |
| eel (anguilla anguilla) and brown trout (salmo trutta) target species to assess the biological impact of trace metal pollution in freshwater ecosystems. | copper, lead, and cadmium pollution were measured in water and sediments of two northern spanish rivers: piles and pigäeña. liver contents of these heavy metals were analyzed in two fish species (eel, anguilla anguilla, and brown trout, salmo trutta) collected from the same locations. significant levels of heavy metal pollution were found in a 38% of fish that are potential catches for sport fishermen. the results indicate that adult eel could be a good metal bioindicator if sampled at a homogen ... | 1996 | 8854823 |
| characterization of the binding specificity of anguilla anguilla agglutinin (aaa) in comparison to ulex europaeus agglutinin i (uea-i). | using immunochemical and immunohistochemical methods, the binding site of anguilla anguilla agglutinin (aaa) was characterized and compared with the related fucose-specific lectin from ulex europaeus (uea-i). in solid-phase enzyme-linked immunoassays, the two lectins recognized fuc alpha 1-2gal beta-hsa. aaa additionally cross-reacted with neoglycolipids bearing lacto-n-fucopentaose (lnfp) i [h type 1] and ii [le(a)] and lactodifucotetraose (ldft) as glycan moieties. uea-i, on the other hand, bo ... | 1996 | 8872115 |
| mediation of serotonin-induced hyperventilation via 5-ht3-receptor in european eel anguilla anguilla. | the effects of serotonin (5-hydroxytryptamine) on ventilation were investigated by continuous measurements of intrabuccal pressure in unrestrained eel. intravenous administration of 5-hydroxytryptamine (30 micrograms.kg-1) caused a large increase in ventilatory frequency (+ 100%) and amplitude (+ 140%). the 5-hydroxytryptamine-induced hyperventilation was blocked by the 5-ht3-receptor antagonists metoclopramide (1.0 mg.kg-1) or mdl72222 (1.0 mg.kg-1), and was insensitive to the 5-ht1/2-receptor ... | 1996 | 8882510 |
| dynamics of anguillicola crassus (nematoda: dracunculoidea) larval infection in paratenic host fishes of lake balaton, hungary. | anguillicola crassus, a nematode parasitizing the swimbladder of the european eel (anguilla anguilla) caused substantial mortality among eels in lake balaton in the years 1991 and 1992. parallel to a 3-year study of the infection dynamics of eels, anguillicola larval infection of paratenic host fishes was also surveyed in the lake between 1991 and 1993. during that study, a total of 1,382 specimens of 22 paratenic host fish species were processed. the results showed that anguillicolosis had beco ... | 1995 | 8882739 |
| importation of aquaculture seedlings to japan. | japan is one of the largest consumers of fishery products in the world. importation of various fishery commodities - including larvae and eggs for aquaculture - is increasing due to high consumer demand. elvers of anguilla anguilla have been imported from europe since the late 1960s. eyed eggs of coho salmon (oncorhynchus kisutch) are imported from north america. during the last decade, over-production caused the price of japanese amberjack (or 'yellowtail') (seriola quinqueradiata) and japanese ... | 1996 | 8890372 |
| primary cultures of dispersed pituitary cells from estradiol-pretreated female silver eels (anguilla anguilla l.): immunocytochemical characterization of gonadotropic cells and stimulation of gonadotropin release. | to analyze the multihormonal control mechanisms of gth secretion in the eel, primary culture of pituitary cells from control or estradiol-treated female silver eels, a treatment known to stimulate in vivo gth synthesis, was developed. dispersed eel pituitary cells obtained by enzymatic (trypsin/dnase) and mechanical dispersion were cultured in earles m199, at 18 degrees. immunoreactive gth (ir-gth) cells were characterized by the immunoperoxidase method, using antiserum to carp gth beta subunit. ... | 1996 | 8921361 |
| anguilla anguilla l. stress biomarkers recovery in clean water and secondary-treated pulp mill effluent. | the eels' recovery from capture, anoxia, and transport is improved by the dechlorinated filtered aerated tap water temperature increase and increased water salinity. the eels' 6-hr recovery from capture, anoxia, and transport, at 25 degrees c in 50% secondary-treated bleached kraft pulp mill effluent, as plasma lactate and cortisol uptake, is depressed. however, either farm or wild eels' exposure to the 50% secondary-treated bleached kraft pulp mill effluent, after 188 hr recovery from capture, ... | 1996 | 8930510 |
| production of recombinant insulin-like growth factor-ii in the development of a radioimmunoassay in rainbow trout (oncorhynchus mykiss). | rainbow trout (oncorhynchus mykiss) insulin-like growth factor-ii (igf-ii) cdna was amplified by reverse transcription-polymerase chain reaction and cloned into an expression vector (parhs3) for production by escherichia coli. recombinant rainbow trout igf-ii (rtigf-ii) was recovered from bacterial inclusion bodies, solubilized, and purified by gel filtration chromatography under reducing conditions. after refolding of the peptide by dialysis at basic ph, we obtained a final yield of 820 microgr ... | 1996 | 8930606 |
| effects of a temperature increase on oxygen consumption of yellow freshwater eels exposed to high hydrostatic pressure. | oxygen consumption was measured in yellow freshwater eels, anguilla anguilla, during exposure to a hydrostatic pressure (hp) of 101 atmosphere absolute (ata) for 3 h, either with or without a 5 degrees c increase in water temperature. it was found that concomitant increases in temperature and pressure resulted in an increase in oxygen consumption that was lower than when pressure alone was increased. when eels were acclimated to hp for 21 days and then submitted to a temperature increase of 5 de ... | 1995 | 8962704 |
| localization of rdna genes in european eel (anguilla anguilla) by fish. | a probe containing most of the transcription unit of the major ribosomal genes of xenopus laevis has been used to localize the rdna cluster of european eel (anguilla anguilla) by fluorescence in situ hybridization (fish). only on nor-bearing chromosome pair has been found. the results obtained with this technique confirm the size polymorphism of the nor revealed previously by other classical cytogenetic techniques. | 1996 | 8983192 |
| colonization and establishment of pomphorhynchus laevis (acanthocephala) in an isolated english river. | the successful colonization, establishment and spread of pomphorhynchus laevis in a small, isolated, devon river 128 km from the parasite's nearest focus in dorset was followed over 11 years from 1985. the parasite was first detected in anguilla anguilla and platichthys flesus in 1988: by 1995 it had attained prevalence levels of 22.6% in a. anguilla and 43.6% in p. flesus and also occurred in 100% salmo trutta, 50% cottus gobio and noemacheilus barbatulus. as judged by prevalence, abundance, pr ... | 1996 | 8991914 |
| potentiating effect of growth hormone on vitellogenin synthesis induced by 17 beta-estradiol in primary culture of female silver eel (anguilla anguilla l.) hepatocytes. | previous in vivo experiments have indicated a potentiating role of growth hormone (gh) during experimentally induced vitellogenesis by 17-beta-estradiol (e2) in the female silver eel (anguilla anguilla l.). to investigate whether gh has direct hepatic actions, the effects of hypophysial-purified and recombinant qh on vitellogenin (vg) synthesis in response to e2 were tested on primary cultures of hepatocytes. hepatocytes were prepared from control or e2-primed eels. addition of e2 alone into the ... | 1996 | 8998971 |
| early emergence of three dopamine d1 receptor subtypes in vertebrates. molecular phylogenetic, pharmacological, and functional criteria defining d1a, d1b, and d1c receptors in european eel anguilla anguilla. | the existence of dopamine d1c and d1d receptors in xenopus and chicken, respectively, challenged the established duality (d1a and d1b) of the dopamine d1 receptor class in vertebrates. to ascertain the molecular diversity of this gene family in early diverging vertebrates, we isolated four receptor-encoding sequences from the european eel anguilla anguilla. molecular phylogeny assigned two receptor sequences (d1a1 and d1a2) to the d1a subtype, and a third receptor to the d1b subtype. additional ... | 1997 | 9006917 |
| effects of diazinon exposure on cholinesterase activity in different tissues of european eel (anguilla anguilla). | cholinesterase (che) activity was measured in brain, plasma, and whole eye of anguilla anguilla experimentally exposed to a sublethal concentration of 0.042 mg/liter (0.50 of the 96-hr lc50) of the organophosphorous pesticide diazinon. whole eye was the tissue which revealed higher values of che activity (8.17 micromol/min/g) in nonexposed animals. brain, plasma, and whole eye che activity of a. anguilla was inhibited at 6, 24, 48, 72, and 96 hr of diazinon exposure. pesticide induced significan ... | 1996 | 9006996 |
| h(+)-glycyl-l-proline cotransport in brush-border membrane vesicles of eel (anguilla anguilla) intestine. | a plasma membrane h(+)-glycyl-l-proline (gly-l-pro) cotransport mechanism has been identified in isolated eel intestinal brush-border membrane vesicles (bbmv) by both measuring radiolabeled gly-l-pro uptake and monitoring gly-l-pro-dependent h+ influx with the ph-sensitive dye acridine orange. the application of an inside negative membrane potential resulted in increasing gly-l-pro uptake, as well as the application of inwardly directed h+ gradient (although only when an inside negative membrane ... | 1997 | 9039012 |
| sublethal effects of an organophosphate insecticide on the european eel, anguilla anguilla. | the present communication deals with the effects of fenitrothion (0.02 mg/liter) on the energy metabolism of the european eel, anguilla anguilla, and its recovery from intoxication. various parameters such as glycogen, lactate, proteins, and glucose levels were measured in different eel tissues after 2, 8, 12, 24, 32, 48, 56, 72, and 96 hr of fenitrothion exposure. subsequently, the fish were allowed recovery periods of 8, 12, 24, 48, 72, 96, 144, and 192 hr in clean water, and the same paramete ... | 1997 | 9056401 |
| angiotensin ii receptors in the gill of sea water- and freshwater-adapted eel. | immunocytochemistry of paraffin-embedded and cryostat sections of eel (anguilla anguilla) gill showed that angiotensin ii receptors (ang ii-r) were present in chloride cells, uniformly distributed in the cytoplasm and on surface membranes. computerised image analysis of these preparations showed that gills from sea water (sw)-adapted animals had a significantly (3-fold) higher ang ii-r concentration compared with freshwater (fw)-adapted eel gills. isoelectric focusing gel electrophoresis reveale ... | 1997 | 9061608 |
| cdna cloning of eel (anguilla anguilla) somatolactin. | the nucleotide sequence of the cdna coding for eel somatolactin (sl), a pituitary hormone belonging to the growth hormone (gh)/prolactin (prl) family, has been determined. the full-length eel sl cdna (1213 bp) encodes a protein of 229 amino acids (aa), with a putative signal peptide of 24 aa and a mature protein of 204 aa. eel sl contains seven cys residues found to be characteristic of sls, and two potential n-glycosylation sites. significant sequence homology between eel and seven other fish s ... | 1997 | 9099860 |
| four polymorphic microsatellite markers in the european eel anguilla anguilla (l.). | 1997 | 9124719 | |
| an electroneutral anion exchange mechanism is present in brush-border membranes isolated from eel kidney. | the mechanism of bicarbonate translocation across the luminal membrane of the eel (anguilla anguilla) kidney tubular cells was studied by monitoring the uptake of h14co3- into isolated brush-border membrane vesicles. results indicate that the presence of a transmembrane outwardly directed cl- gradient was able to transiently accumulate h14co3- into the vesicular space, whereas neither an inwardly directed sodium gradient nor a transmembrane electrical potential gradient (inside positive) was abl ... | 1997 | 9140013 |
| composition and structure of helminth communities in eels anguilla anguilla from italian coastal lagoons. | the composition and diversity of total and intestinal helminth component and infracommunities were determined in eels anguilla anguilla from four shallow coastal lagoons near rome. the lagoons differed principally in respect of their salinity. only lake burano still received an input of freshwater, and both freshwater and marine helminth species were found in eels. in the other three lagoons all helminth species were marine or euryhaline and communities were dominated by digeneans. at component ... | 1997 | 9166436 |
| residue study of mebendazole and its metabolites hydroxy-mebendazole and amino-mebendazole in eel (anguilla anguilla) after bath treatment. | mebendazole (mbz) is extensively used in eel culture for treatment of pseudodactylogyrus spp. infections. this use may lead to residues of mbz in eel tissues. consequently, the residue profile of mbz in eel after treatment with the drug is of special concern. therefore, a residue study was performed in european eels (anguilla anguilla), bath-treated with mbz at a dose of 1 mg/liter for 24 hr and kept at a water temperature of 25 degrees c. liver, kidney, fat, skin, and muscle tissues samples wer ... | 1997 | 9172949 |
| the anodic hemoglobin of anguilla anguilla. molecular basis for allosteric effects in a root-effect hemoglobin. | the functional and structural basis for the root effect has been investigated in the anodic hemoglobin of the european eel, anguilla anguilla. this hemoglobin exhibits a large bohr effect, which is accounted for by oxygen-linked binding of seven to eight protons in the presence of gtp at ph 7.5. oxygen equilibrium curves show nonlinear lower asymptote of hill plots, indicating the occurrence of heme-heme interactions within the t state. analysis of the curves according to the co-operon model (br ... | 1997 | 9188451 |
| similarities and differences between the massive eel (anguilla anguilla l.) devastations that occurred in lake balaton in 1991 and 1995. | in the past few years, two massive eel (anguilla anguilla l.) devastations occurred in lake balaton, hungary. in 1991, 300 tons of eel perished in the western basin of the lake, while in the summer of 1995 30 tons of eel died in the eastern part of the lake. investigations carried out to find the causes of these ecocatastrophes included measurements of certain biochemical parameters: the blood sugar level, and the acetylcholinesterase (ache, ec 3.1.1.7), lactate dehydrogenase (ldh, ec 1.1.2.3), ... | 1997 | 9212331 |
| changes in the electrophysiological parameters of the posterior intestine of anguilla anguilla (pisces) induced by oxytocin, urotensin ii and aldosterone. | in view of the importance of the intestine in the osmoregulation of freshwater fishes, we determined the effects of oxytocin, urotensin ii (uii), and aldosterone added to the serosal side of the isolated posterior intestine of the freshwater-adapted teleost anguilla anguilla on electrophysiological parameters. oxytocin decreased the short-circuit current (scc) and transepithelial potential difference (tpd) at concentrations of 1 and 10 mu/ml (to 50% and 42% of control values, respectively), but ... | 1997 | 9222401 |
| endoparasitic helminths of the european eel, anguilla anguilla, from four disconnected meanders from the rivers leie and scheldt in western flanders, belgium. | the endoparasitic helminth communities of the european eel, anguilla anguilla (l.), were investigated in four meanders, cut off from the rivers leie and scheldt in western flanders, belgium. six species of helminths (2 cestodes, 2 nematodes and 2 acanthocephalans) were found. the dominant parasite species was the nematode anguillicola crassus (kuwahara, niimi et itagaki, 1974) infecting 79% of the eel population with intensities up to 112 specimens per fish. at two localities no acanthocephalans ... | 1997 | 9229569 |
| habitat use by the fish assemblages of two chalk streams | patterns of habitat use by fish assemblages in two chalk streams in southern england were examined to identify species preferences with respect to major habitat gradients. both study sites, although differing in some physical habitat characteristics, mainly channel width, water temperature and instream cover, could be arranged similarly along a continuum extending from erosional to depositional habitats. twelve fish species were collected from stream surveys conducted during july 1993. the habit ... | 1997 | 9236089 |
| histopathological changes induced by environmental stress in common carp, japanese coloured carp, european eel, and african catfish. | histopathological changes caused by stress during catching and transport were examined in four fish species, the common carp (cyprinus carpio), the japanese coloured carp (koi, the coloured variant of the asian carp, cyprinus carpio haematopterus), the european eel (anguilla anguilla), and the african catfish (clarias gariepinus). in all species, the goblet cells of the gills and skin were decreased in number and slight detachment of the epithelium of the secondary gill lamellae was seen. loss o ... | 1997 | 9270124 |
| epidermal proteinases in the european eel. | a fluorescent-sensitive assay demonstrated the exhaustive detection of proteinase activities in the dorsal skin of the european eel. two distinct skin extracts were prepared from skin mucus and epidermal cell layers with no mutual contamination, so that the latter extract contained significant susceptibility of all tested substrates. optimum hydrolysis ph's for susceptible substrates were found in acidic and neutral ranges, and optimum hydrolysis temperatures for the same substrates fell mainly ... | 2006 | 9279923 |
| androgens stimulate gonadotropin-ii beta-subunit in eel pituitary cells in vitro. | primary cultures of juvenile eel (anguilla anguilla) pituitary cells were used to study the direct effects of sex steroids on gonadotropin (gth-ii) cell content and release (radioimmunoassay) as well as on mrnas levels for alpha and gth-ii beta-subunits (dot-blot). testosterone stimulated gth-ii production in a dose- and time-dependent manner by selectively increasing mrnas for gth-ii beta-subunit but not alpha-subunit. this positive effect was also induced by non-aromatizable androgens (androst ... | 1997 | 9296374 |
| ion transport in the intestine of anguilla anguilla: gradients and translocators | the transport of na+, k+ and cl- across the isolated epithelium of the eel intestine was studied using a combination of four experimental strategies: short-circuiting, measurements of intracellular potentials and ion concentrations, application of a variety of transport inhibitors and measurement of unidirectional fluxes with radioactive tracers. when short-circuited, the system performs a net transport of cl- and na+ towards the blood side, with a stoichiometry approaching 2, and a much smaller ... | 1994 | 9317418 |
| rapid and simultaneous measurement of anodic and cathodic haemoglobins and atp and gtp concentrations in minute quantities of fish blood | oxygen transport to vertebrate tissues is dependent upon (a) the intrinsic o2-binding properties of haemoglobin (hb) and (b) modulation by allosteric effectors, such as nucleoside triphosphates (ntp), that depress hb o2-affinity in fish red cells. while some species, such as flatfish, primarily use adenosine triphosphate (atp) to modulate o2-binding to hb, others, such as eel and carp, also use guanosine triphosphate (gtp) (kono and hashimoto, 1977; leray, 1982; weber and jensen, 1988). unlike m ... | 1994 | 9317794 |
| the erythrocyte na+/h+ exchangers of eel (anguilla anguilla) and rainbow trout (oncorhynchus mykiss): a comparative study | trout and eel red blood cell na+/h+ exchangers show widely different regulatory properties. catecholamines, cyclic amp and phorbol esters, which activate the trout red cell antiporter, do not affect the eel exchanger. unlike the trout red cell exchanger, the eel red cell exchanger is strongly activated by cell shrinkage, allowing a remarkable cell volume recovery. these different regulatory properties probably indicate the existence of different isoforms of the exchangers in nucleated erythrocyt ... | 1996 | 9318046 |
| bioconcentration and excretion of fenitrothion in the brain of the eel (anguilla anguilla). | the bioconcentration of fenitrothion in the brain of the european eel (anguilla anguilla) and its posterior elimination have been studied. animals were exposed to a sublethal concentration of fenitrothion (0.04 mg/l) for 96 hours in a flow-through test system. after this pesticide exposure, animals were transferred to clean water for 72 hours more. bioconcentration and elimination processes of fenitrothion were studied in blood and brain. this insecticide showed a strong tendency to bioconcentra ... | 1997 | 9350078 |
| response and recovery of acethylcholinesterase activity in the european eel anguilla anguilla exposed to fenitrothion. | european eel (anguilla anguilla) were exposed to sublethal fenitrothion concentrations in a continuous flow-through system for 4 days. muscle acetylcholinesterase (ache) activity was evaluated after 2, 8, 12, 24, 32, 48, 56, 72 and 96 hours pesticide exposure. results showed that ache activity in eel muscle tissue decreased as concentration of fenitrothion increased. pesticide induced significant inhibitory effects on the ache activity of a. anguilla ranging from > 35% inhibition at sublethal co ... | 1997 | 9350079 |
| down-regulation of tsh subunit mrna levels by thyroid hormones in the european eel. | the effects of 3,5,3'-triiodothyronine (t3) and thyroxine (t4) on alpha and thyroid-stimulating hormone (tsh) beta subunit mrna pituitary levels were examined in a teleost, the european silver eel. northern blot analysis showed that the number and length of mrnas encoding tsh beta varied among individuals, a variability apparently not related to thyroidal status. when several bands were present, their intensities were summed for quantitative analysis. increasing circulating thyroid hormones (ths ... | 1997 | 9356215 |
| effects of vagal stimulation on swimbladder blood flow in the european eel anguilla anguilla. | the influence of the vagus nerve on swimbladder blood flow in the european eel (anguilla anguilla) was characterized by recording the changes in blood flow rate and blood pressure following stimulation of the vagus nerve. after electrical stimulation, blood flow in the swimbladder artery increased from 0.9 ml min-1 to 2.1 ml min-1. video recordings of small vessels on the caudal side of the rete mirabile revealed an increase in erythrocyte velocity combined with a small vasodilation. this effect ... | 1997 | 9364021 |
| ph-dependent proton secretion in cultured swim bladder gas gland cells. | the ph dependence of acid production and of acid release has been analyzed in cultured gas gland cells of the european eel using a cytosensor microphysiometer. total acid release of gas gland cells showed an optimum at ph 7.4-7.6, with only a minor reduction at acidic (ph 7.0) as well as at alkaline ph (ph 8.0). the acid production was largely dependent on the availability of extracellular glucose and was almost completely abolished if glucose was replaced by succinate, alanine, or even pyruvate ... | 1997 | 9374815 |
| inhibition of gill na+,k+-atpase activity in the eel, anguilla anguilla, by fenitrothion. | european eels (anguilla anguilla) were exposed to sublethal fenitrothion concentrations (0.02 and 0.04 mg/liter) in a continuous flow-through system for 4 days. gill mg2+- and na+,k+-atpase activities were evaluated after 2, 8, 12, 24, 32, 48, 56, 72, and 96 h of pesticide exposure. results indicated that atpase activity in gill tissue decreased as concentration of fenitrothion increased. pesticide induced significant inhibitory effects on the na+, k+-atpase activity of a. anguilla, ranging from ... | 1997 | 9417854 |
| isolation of six putative p-type atpase beta subunit pcr fragments from the brain of the european eel (anguilla anguilla). | 1997 | 9432906 | |
| long-term and seasonal changes in composition and richness of intestinal helminth communities in eels anguilla anguilla of an isolated english river. | the need for more long-term studies on helminth communities was addressed by examining changes in composition and diversity of the intestinal helminth component and infracommunities in eels of a small isolated river over 12 years. examination of samples over one summer season indicated that single samples were representative of community richness in that year. in 1985 the community was species poor (1 species only) and with zero diversity, but by 1996 it comprised six species and all parameters ... | 1997 | 9437839 |
| glutathione-s-transferase is an important antigen in the eel nematodeanguillicola crassus | different organs and secretions/excretions of the swimbladder parasite, anguillicola crassus (nematoda), were tested for the presence of antigens to the humoral immune response previously detected in the european eel, anguilla anguilla. proteins from different fractions of anguillicola crassus were separated using sds-page (sodium-dodecyl-sulphate polyacrylamide-gel electrophoresis) under reducing conditions and electroblotted onto nitrocellulose membranes. infected eels showed a specific antibo ... | 1997 | 9443950 |
| lectin histochemistry reveals the appearance of m-cells in peyer's patches of scid mice after syngeneic normal bone marrow transplantation. | peyer's patches in the intestinal mucosa are characterized by the presence of several lymphatic follicles and interfollicular t-cell regions. luminal antigens are transported across the intestinal epithelium to stimulate the peyer's patch pre-b-cells in the follicles that proliferate and migrate to distant sites. evidence suggests that antigen priming of b-lymphocytes is responsible for the number and location of peyer's patches during postnatal life, but little is known about the histogenesis o ... | 1998 | 9446820 |
| response and recovery of brain acetylcholinesterase activity in the european eel, anguilla anguilla, exposed to fenitrothion. | european eel (anguilla anguilla) were exposed to sublethal fenitrothion concentrations in a continuous flow-through system for 4 days. brain acetylcholinesterase (ache) activity was evaluated after 2, 8, 12, 24, 32, 48, 56, 72, and 96 h pesticide exposure. results indicated that ache activity in eel brains decreased as the concentration of fenitrothion increased. the pesticide induced significant inhibitory effects on the ache activity of a. anguilla, ranging from > 40% inhibition at a sublethal ... | 1997 | 9469870 |
| induction of erod activity and genotoxic effects by polycyclic aromatic hydrocarbons and resin acids on the juvenile eel (anguilla anguilla l.). | the purpose of this research is the study of total ethoxyresorufin o-deethylase (erod) activity and erythrocytic nuclear abnormalities (na) induction by polycyclic aromatic hydrocarbons (pahs) and resin acids (ras) on the juvenile eel anguilla anguilla l. juvenile eels were exposed, during 3 days, to the same molar concentration range (0, 0.1, 0.3, 0.9, and 2.7 microm) of beta-naphthoflavone (bnf) and benzo[a]pyrene (bp)--pahs--and abietic acid (aa) and dehydroabietic acid (dhaa)--ras--and one a ... | 1997 | 9469877 |
| uptake and elimination kinetics of a pesticide in the liver of the european eel. | this study was undertaken to determine the bioconcentration-elimination process of fenitrothion in the liver of the european eel (anguilla anguilla). the animals were exposed to two sublethal fenitrothion concentrations corresponding to 1/5 and 1/10 of lc50 96-h in a flow-through test system. uptake kinetics were determined from liver burdens measured at 2, 8, 24, 48, 56, 72 and 96 hours exposure. after pesticide exposure, animals were transferred to clean water for 72 hours. fenitrothion elimin ... | 1998 | 9491569 |
| studies on the eel sperm flagellum. 2. the kinematics of normal motility. | the sperm flagellum of anguilla anguilla lacks outer dynein arms, radial spokes and central structures. its characteristic motion has been obtained by studying cells swimming perpendicularly against, but not adhering to, the coverslip. the flagellum generates a sinistrally helical wave of rising, then falling, amplitude. the frequency of the wave, which can exceed 70 hz, is inversely related to its maximum amplitude. as a reaction to the torque, the entire cell rolls (spins) in the opposite dire ... | 1998 | 9519904 |
| attempts to analyse anguillicola crassus infection and the humoral host response in eels (anguilla anguilla) of lake balaton, hungary. | since the introduction of anguillicola crassus into europe, anguillicolosis has been a considerable problem in several countries. from 1991, periodical eel mortality occurred in lake balaton, hungary. however, eels with a worm burden of 20 to 50 parasites did not show severe swimbladder lesions, which observation cast doubts on the primary aetiological role of the parasite in the eel kill. in order to study the pathology of the infection, from the spring of 1996 until october of the same year, 5 ... | 1997 | 9557321 |
| sublethal bioconcentration of fenitrothion in the blood and brain of the european eel. | 1998 | 9595200 | |
| induction of liver erod and erythrocytic nuclear abnormalities by cyclophosphamide and pahs in anguilla anguilla l. | the induction of liver ethoxyresorufin o-deethylase (erod) activity and erythrocytic nuclear abnormalities (na) after treatment with two different polycyclic aromatic hydrocarbons (pahs) and cyclophosphamide (cp) was investigated in the eel anguilla anguilla l. erod activity significantly increases either 3 days after one single i.p. injection or 6 days after two i.p. injections (on days 0 and 3) of 4 mg/kg beta-naphthoflavone (bnf). erod activity was determined after different lengths of exposu ... | 2013 | 9626538 |
| laboratory and field-caging studies on hepatic enzymatic activities in european eel and rainbow trout. | caging field experiments were conducted on young european eel (anguilla anguilla) and on rainbow trout (onchorynchus mykiss) for the determination of a biomonitoring protocol of freshwater streams. hepatic monooxygenase and conjugation enzyme activities were measured as biomarkers of exposure to some organic pollutants. laboratory studies were performed to compare the responsiveness of these two species concerning their enzymatic activities. the induction of monooxygenase activity, measured as t ... | 2015 | 9626548 |
| switch in rod opsin gene expression in the european eel, anguilla anguilla (l.). | the rod photoreceptors of the european eel, anguilla anguilla (l.), alter their wavelength of maximum sensitivity (lambda max) from c.a. 523 nm to c.a. 482 nm at maturation, a switch involving the synthesis of a new visual pigment protein (opsin) that is inserted into the outer segments of existing rods. we artificially induced the switch in rod opsin production by the administration of hormones, and monitored the switch at the level of mrna accumulation using radiolabelled oligonuleotides that ... | 1998 | 9633112 |
| long-term inhibitory effects of somatostatin and insulin-like growth factor 1 on growth hormone release by serum-free primary culture of pituitary cells from european eel (anguilla anguilla). | to investigate the ability of hypothalamic and peripheral factors to directly regulate growth hormone (gh) release in a primitive teleost, the european eel (anguilla anguilla l.), we used primary cultures of dispersed pituitary cells. when cultured for 12 days in a serum-free medium, pituitary cells continuously released large amounts of gh, which exceeded the initial cellular content. somatotropin-release inhibiting hormone (srih-14) dose-dependently inhibited gh release (ec50 0.75 nm) up to a ... | 1998 | 9641611 |
| distribution and prevalence of anguillicola crassus (nematoda) in eels anguilla anguilla of the rivers rhine and naab, germany. | over a period of 2 yr eels were collected at several sites along the river rhine as well as from adjacent waters and examined for anguillicola crassus in the swimbladder. additionally eels were collected once from a site on the river naab (danube system). all eels were searched for both juvenile and adult stages of a. crassus, revealing prevalences of 80% in eels from the river rhine and 39% in eels from the river naab. the mean intensities were 5.6 and 7.7, respectively. with respect to the pre ... | 1998 | 9676253 |
| tyrosine hydroxylase in the european eel (anguilla anguilla): cdna cloning, brain distribution, and phylogenetic analysis. | we report the isolation of a full-length eel tyrosine hydroxylase (th) cdna that is characterized by a long 3' untranslated region and by a diversity restricted to the 3' end owing to the differential use of three polyadenylation signals. the longest eel th mrna was distinctive in the presence of four pentameric elements (auuua) in the au-rich 3' noncoding region. such a diversity could provide the basis of posttranscriptional or translational regulation of eel th gene expression. comparison of ... | 1998 | 9681435 |
| hydrogen ion titrations of the anodic and cathodic haemoglobin components of the european eel anguilla anguilla. | h+ titrations were conducted on the separated haemoglobin components of eel anguilla anguilla in both the oxygenated and deoxygenated states. in anodic haemoglobin, the addition of gtp, and to a lesser extent c1-, increased the magnitude of the haldane effect and shifted its maximum value into the in vivo ph range. of the 22 histidine residues in the anodic component, only approximately seven were titratable, presumably the beta-chain residues at positions 41, 97, 109 and 146 (helical positions ... | 1998 | 9698585 |
| heterogeneity of eel thyrotropin beta mrnas is due to a minisatellite in the 3' untranslated region of the gene. | the aim of this study was to determine the causes of the high heterogeneity, in the number and the length, of the thyrotropin (tsh) beta mrna in the european eel. northern blot analysis showed that removal of the poly(a) tail did not affect this heterogeneity. pcr amplification on reverse-transcribed pituitary rnas (rt) showed the main source of heterogeneity to be a highly variable region in the 3' untranslated region (utr). pcr amplification of the 3' utr from rts and genomic dnas demonstrated ... | 1998 | 9714823 |
| impact of low water temperature on the development of anguillicola crassus in the final host anguilla anguilla. | the effect of low water temperatures on the development and viability of larval and adult anguillicola crassus (nematoda) in the final host anguilla anguilla was studied. european eels were experimentally infected with a. crassus and then maintained for 4 mo at 4, 9, 10, and 19 degrees c. larval development showed a temperature-dependent pattern and was significantly retarded at low temperatures. third-stage larvae survived a 4 mo period at 4 degrees c without being affected, although they were ... | 1998 | 9722403 |
| distribution, characterization, and growth hormone-releasing activity of pituitary adenylate cyclase-activating polypeptide in the european eel, anguilla anguilla. | the complementary dna encoding pituitary adenylate cyclase-activating polypeptide (pacap) has been cloned from two species of teleost fishes, the sockeye salmon and the thai catfish, and the amino acid sequence of pacap has been determined in another teleost, the stargazer. however, to date, the detailed distribution of pacap immunoreactivity has never been investigated in the fish brain. in the present study, we have determined the localization of pacap-immunoreactive neurons in the central ner ... | 1998 | 9751513 |
| liver energy metabolism of anguilla anguilla after exposure to fenitrothion. | this paper deals with the effect of fenitrothion (0.04 mg/liter) on the energy metabolism of the european eel, anguilla anguilla, and its recovery from intoxication. various parameters such as glycogen, lactate, proteins, total lipids, and glucose in eel liver and blood were analyzed after 2, 8, 12, 24, 32, 48, 56, 72, and 96 h of fenitrothion exposure. subsequently, the fish were allowed recovery periods of 8, 12, 24, 48, 72, 96, 144, and 192 h in clean water, and the same parameters were evalu ... | 1998 | 9756704 |
| electroretinographic evaluation of spectral sensitivity in yellow and silver eels (anguilla anguilla). | although differences in visual pigments between developmental stages of the european eel are well known, the expected differences in spectral sensitivity have not been demonstrated at the electrophysiological level. in fact, one past electroretinographic study led to the conclusion that in eels there is no change in scotopic sensitivity, with increasing sexual maturity. in the present experiments, electroretinograms (ergs) were recorded from in situ eyecups of immobilized eels anguilla anguilla ... | 2007 | 9764534 |
| adenylyl cyclase activity and glucose release from the liver of the european eel, anguilla anguilla. | the properties of adenylyl cyclase (ac) in liver membranes of the european eel (anguilla anguilla) and the involvement of camp in glucose release from isolated hepatocytes in response to catecholamines were studied. basal enzyme activity seemed essentially unaffected by gtp, while a biphasic response to increasing nucleotide concentrations was obtained in the presence of epinephrine. eel liver ac was dose-dependently stimulated by guanosine 5'-o-(3-thiotriphosphate) and inhibited by guanosine 5' ... | 1998 | 9791074 |
| insulin-like growth factor-i stimulates gonadotrophin production from eel pituitary cells: a possible metabolic signal for induction of puberty. | insulin-like growth factor (igf)-i has been suggested as a potential signal linking growth and puberty in mammals. using the juvenile european eel as a model, we employed a long-term, serum-free primary culture of pituitary cells to study the direct effect of igf-i on gonadotrophin (gth-ii=lh) production. igf-i increased both cell content and release of gth-ii in a time- and dose-dependent manner. igf-i and igf-ii had similar potencies but insulin was 100-fold less effective, suggesting the impl ... | 1998 | 9795340 |
| pesticide toxicokinetics in fish: accumulation and elimination. | bioaccumulation of fenitrothion in the european eel (anguilla anguilla) was studied using two sublethal concentrations of this pesticide in a flow-through test system. the pesticide concentrations used were one-tenth (0.002 ppm) and one-fifth (0.04 ppm) the 96-h lc50 of fenitrothion in this species. steady state was reached early (2 h) when the animals were exposed to 0.02 ppm of toxicant, and after 48 h when the animals were exposed to 0.04 ppm. toxicokinetic parameters for fenitrothion in eel ... | 1998 | 9799575 |
| redescription of rhabdochona anguillae (nematoda: rhabdochonidae), a parasite of eel, anguilla anguilla, in europe. | the nematode rhabdochona anguillae spaul, 1927, a specific intestinal parasite of the european eel, anguilla anguilla (l.), is redescribed and illustrated from specimens collected from eels of the sousa river, northern portugal (prevalence 20%, intensity 2-13). the species is characterized by the presence of 14 anterior teeth in the prostom, small non-bifurcate deirids, absence of lateral preanal papillae, by the length (0.460-0.660 and 0.130-0.150 mm) and the shape of spicules, fairly large (0. ... | 1998 | 9805785 |
| different putative neuromodulators are present in the nerves which distribute to the teleost skeletal muscle. | the presence of putative neuromodulators in the nerve fibres was investigated in white skeletal muscle of two teleost fish not taxonomically correlated and showing different patterns of innervation (multiple versus focal innervation). cryostat sections of epaxial, hypaxial and adductor mandibulae (am) muscles of sparus aurata and anguilla anguilla were stained histochemically for reduced nicotinamide adenine dinucleotide phosphate (nadph)-diaphorase. other sections were used for indirect immunoh ... | 1998 | 9810486 |