Publications

random amplified polymorphic dna analysis of eel genome.	eel family is a huge one, in which many kinds of eels especially some migratory eels, bear strong resemblance to each other, and are therefore difficult to be identified. in this study 29 random primers were used to make rapd analysis for japanese eel (anguilla japonica), european eel (anguilla anguilla) and pike eel (muraenesox cinereus). and totally 299 fragments were counted. shared or specific fragments were counted and genetic similarity or genetic distance were calculated. the genetic simi ...	1999	10520604
effects of sea water and stanniectomy on branchial ca(2+) handling and drinking rate in eel (anguilla anguilla l.).	we examined the effects of seawater adaptation and extirpation of the stannius corpuscles on branchial ca(2+) flows, gill plasma membrane ca(2+) transporters and drinking rate of european eels, anguilla anguilla. transepithelial ca(2+) inflow in the gills increased 2 weeks after transfer of the eels from fresh water to sea water and after stanniectomy. neither of these treatments changed the membrane density or the affinity of the ca(2+)-extrusion mechanisms (ca(2+)-atpase and na(+)/ca(2+)-excha ...	1999	10460737
pharmacokinetic and depletion studies of sarafloxacin after oral administration to eel (anguilla anguilla).	the pharmacokinetics of sarafloxacin applied by oral gavage at a dose of 15 mg/kg b.w. was studied in eel (anguilla anguilla) at water temperature of 24 degrees c. sarafloxacin levels were determined using high performance liquid chromatography with a quantitation limit of 0.07 microg/ml or gram. the time to peak plasma concentration, tmax, was 12 hr and peak concentration, cmax, was 2.64 microg/ml. the absorption rate constant (k(a)) was 0.23 hr(-1) (r=0.996). the drug disposition curve after t ...	1999	10379934
a kinematic comparison of forward and backward swimming in the eel anguilla anguilla	in addition to forward undulatory swimming, eels (and some other elongated swimmers) can swim backwards in a similar way. we compared the kinematics (wave speed, cycle frequency, amplitude, local bending and estimated muscle strain) of forward and backward swimming in the european eel anguilla anguilla. both swimming modes are characterised by a wave of undulation that travels over the body in the direction opposite to that of swimming. we observe two major kinematic differences. first, the slop ...	1999	10229697
localization of carbonic anhydrase in swimbladder of european eel (anguilla anguilla) and perch (perca fluviatilis).	the distribution of carbonic anhydrase in swimbladder tissue and especially in gas gland cells of the european eel (anguilla anguilla) and the perch (perca fluviatilis) was analysed using histochemical staining according to hansson (1967), with modifications proposed by riddersträle (1991). while in the european eel, gas gland cells are distributed as a single layered epithelium over the whole secretory part of the swimbladder, the gas gland of the perch consists of a compact, richly vascularize ...	1999	10090334
vasodilation of swimbladder vessels in the european eel (anguilla anguilla) induced by vasoactive intestinal polypeptide, nitric oxide, adenosine and protons	the effects of &bgr;-adrenergic stimulation, vasoactive intestinal polypeptide (vip), adenosine, the nitric oxide (no)-releasing agent sodium nitroprusside and of metabolic end-products of gas gland cell metabolism on swimbladder blood flow were investigated using saline- or blood-perfused swimbladder preparations of the freshwater european eel anguilla anguilla. while &bgr;-adrenergic vasodilation was not detectable, a bolus injection of adenosine (100 microl, 10(-)7 mol l-1) and application of ...	1999	10085273
cortisol selectively stimulates pituitary gonadotropin β-subunit in a primitive teleost, anguilla anguilla.		1999	28200866
thiobencarb toxicity and plasma ache inhibition in the european eel.	the acute toxicity of the herbicide thiobencarb (s-4-chlorobenzyl diethylthiocarbamate) was determined for the european eel (anguilla anguilla). the 24, 48, 72 and 96 hours median lethal concentrations (lc50) were 25.7, 21.7, 17.0 and 13.2 mg/l, respectively. fish were also exposed to a sublethal thiobencarb concentration (1/60 lc50-96 hr = 0.22 mg/l) during 96 hours in a flow-through system and then an elimination period of 192 hours in clean water was allowed. eels were removed and blood sampl ...	1999	10048205
chloride channels in the eel intestine	patches of freshly isolated epithelial cells from eel anguilla anguilla intestine bathed by the same solution on both sides in the cell-attached configuration had conductances of 57.0+/-1.8 ps (for positive voltages) and 13.3+/-0.7 ps (for negative voltages) (means +/- s.e.m., n=25). electrical activity was spontaneous in the cell-attached configuration, but was frequently lost after excision. in inside-out patches, channel activity was restored by strong hyperpolarization (-150 mv for 5 s) or d ...	1999	9841893
pcdds/pcdfs and coplanar pcbs in eels (anguilla anguilla) from different areas of the rivers havel and oder in the state of brandenburg (germany).	in 1996 forty-nine eels were caught from different locations along the rivers oder (hohenwutzen and schwedt) and from 11 locations along the havel river. they were analysed for pcdds/pcdfs and the coplanar pcbs (pcb 77, pcb 126, pcb 169). their contribution to 2,3,7,8-tcdd equivalents were estimated. in case of pcdds/pcdfs these amounted between 1.8 and 15.2 pg/g fat (mean 6.1 pg/g; median 5.2 pg/g), in case of coplanar pcbs between 2.4 and 170.5 pg/g fat (mean 47.7 pg/g; median 36.1 pg/g). lake ...	1999	10901658
development of anguillicola crassus (dracunculoidea, anguillicolidae) in experimentally infected balearic congers ariosoma balearicum (anguilloidea, congridae).	the development of anguillicola crassus in experimentally infected ariosoma balearicum (anguilloidea, congridae) kept in seawater was studied in the laboratory. in parallel trials the effect of water salinity on the development of larval a. crassus in european eels anguilla anguilla was also investigated using eels kept in seawater of a salinity of 34 per thousand. both eel species were orally inoculated with l3 larvae of a. crassus and then maintained for up to 3 mo at 18 degrees c in seawater. ...	1999	11407407
differentiation of helicobacter pylori isolates based on lectin binding of cell extracts in an agglutination assay.	plant and animal lectins with various carbohydrate specificities were used to type 35 irish clinical isolates of helicobacter pylori and the type strain nctc 11637 in a microtiter plate assay. initially, a panel of eight lectins with the indicated primary specificities were used: anguilla anguilla (aaa), lotus tetragonolobus (lotus a), and ulex europaeus i (uea i), specific for alpha-l-fucose; solanum tuberosum (sta) and triticum vulgaris (wga), specific for beta-n-acetylglucosamine; glycine max ...	1999	10325361
biochemical and genotoxic responses of adult eel (anguilla anguilla l.) to resin acids and pulp mill effluent: laboratory and field experiments.	the potential of a secondary-treated bleached kraft pulp mill effluent (bkpme) and resin acids (ras) to induce liver ethoxyresorufin-o-deethylase (erod) activity and erythrocytic nuclear abnormalities (enas) in adult anguilla anguilla l. was investigated in laboratory and field experiments. hepatic health was assessed by measurement of liver alanine transaminase (alt). one single intraperitoneal injection of abietic acid (aa) or dehydroabietic acid (dhaa), at the same molar dose (14.7 micromol/k ...	1999	9931243
cortisol selectively stimulates pituitary gonadotropin beta-subunit in a primitive teleost, anguilla anguilla.	it has been suggested that in mammals, glucocorticoids, beside their stress-related inhibitory effects on reproductive function, may also play a stimulatory role at the onset of puberty. using the juvenile female eel as a model, we investigated the potential stimulatory role of cortisol (f) on pituitary gonadotropin (gth-ii). gth-ii levels were measured by ria, and messenger rna (mrna) levels for alpha- and gth-ii beta-subunits were determined by dot blot using homologous probes. f treatment inc ...	1999	10067848
isolation and characterization of vibrio parahaemolyticus causing infection in iberian toothcarp aphanius iberus.	high mortality among laboratory cultured iberian toothcarp aphanius iberus occurred in february 1997 in valencia (spain). the main signs of the disease were external haemorrhage and tail rot. bacteria isolated from internal organs of infected fish were biochemically homogeneous and identified as vibrio parahaemolyticus. the bacteria were haemolytic against erythrocytes from eel anguilla anguilla, amberjack seriola dumerili, toothcarp a. iberus and humans, and were kanagawa-phenomenon-negative. i ...	1999	10073316
density-dependent effects on anguillicola crassus (nematoda) within its european eel definitive host.	density-dependent effects on adult and larval stages of the introduced nematode parasite anguillicola crassus in the european eel definitive host were examined in the laboratory in naturally infected fish, and in field samples. the effect of adult nematode subpopulations on larval development over time and the effect of increasing adult intensity on the gravid female subpopulations were investigated. at high adult subpopulations the movement of larvae from the swimbladder wall into the swimbladd ...	1999	10205805
characterization by enzyme electrophoresis of specimens of the genus helicometra (trematoda, opecoelidae) from fish caught off the coast of northwest spain.	species within the genus helicometra are difficult to distinguish on morphological grounds alone, and are best discriminated with the aid of biochemical techniques. in the work reported here, the electrophoretic mobility of malate dehydrogenase isoenzyme of the mdh-1 locus was used to characterize 375 individuals of helicometra obtained from various teleost species (anguilla anguilla, conger conger, gobius niger or ciliata mustela) caught off the coast of northwest spain. the results suggest tha ...	1999	10229945
evidence that corticotropin-releasing hormone acts as a growth hormone-releasing factor in a primitive teleost, the european eel (anguilla anguilla).	the inhibitory control of growth hormone (gh) release by somatostatin (srih) has been conserved throughout vertebrate evolution. in contrast, the neuropeptides involved in the stimulatory control of gh vary according to species and/or physiological situations. we investigated the direct pituitary regulation of gh release in a primitive teleost, the european eel (anguilla anguilla l.) at the juvenile stage. short-term serum-free primary cultures of dispersed pituitary cells were used, and gh rele ...	1999	10320566
experimental studies on lead accumulation in the eel-specific endoparasites anguillicola crassus (nematoda) and paratenuisentis ambiguus (acanthocephala) as compared with their host, anguilla anguilla.	the effect of salinity and the mode of application (oral versus aqueous) on the lead accumulation in different tissues of the fish host eel (anguilla anguilla) and its parasites anguillicola crassus (nematoda) and paratenuisentis ambiguus (acanthocephala) was investigated. waterborne as well as dietary lead exposure caused an increase in the metal levels of different eel tissues and the parasites. the mode of lead application had a significant influence on the distribution of lead in the fish ti ...	1999	10398769
osmotic equilibrium and elastic properties of eel intestinal vesicles.	changes in volume of intestinal brush border membrane vesicles of the european eel anguilla anguilla were measured as vesicles were exposed to media with different osmotic pressures. preparing the vesicles in media of low osmotic pressure allowed the effects of a small hydrostatic pressure to become a significant factor in the osmotic equilibration. by applying laplace's law to relate pressure and volume and assuming a linear relation between membrane tension and area expansion, we estimate an i ...	1999	10489428
contents of several inorganic substances in european eel infected and uninfected by anguillicola crassus (nematoda).	the content of 5 macroelements and 5 microelements were analyzed using the atomic absorption method in muscle samples of european eels infected and uninfected by anguillicola crasus. the mean contents of these substances in infected eels were statistically highly significantly lower in ca, p, fe, mn, but only statistically significantly lower in na, mg, zn and cu as compared to uninfected fishes. these differences are discussed in relation to hematophagus feeding and pathogenity of the parasite.	1999	10494503
richness and diversity of parasite communities in european eels anguilla anguilla of the river rhine, germany, with special reference to helminth parasites.	a total of 121 european eels (anguilla anguilla) from 2 sampling sites on the river rhine were investigated in respect of their parasite communities. special attention was given to the swim bladders, intestines, gills and fins of the fish. twelve different parasite species were found to live in and on the eels. data from each sampling site were kept separate. parasites found in descending order of prevalence were: anguillicola crassus, trypanosoma granulosum, myxobolus sp., paratenuisentis ambig ...	1999	10503258
levels of synthetic musks; bromocyclene and pcbs in eel (anguilla anguilla) and pcbs in sediment samples from some waters of berlin/germany.	the purpose of this study was to identify firstly the extent of contamination both of the aquatic biota and of sediments and secondly any regional differences in the concentrations of these compounds. in 1996, polychlorinated biphenyls (pcbs, including their coplanar congeners) were measured in 58 eel and 50 sediment samples. furthermore, the veterinary pharmacetical bromocyclene and the nitro musks were determined in 84 (1995) and 122 (1996) eel samples. polycyclic musk fragrances were addition ...	1999	10520489
an enhanced humoral immune response against the swimbladder nematode, anguillicola crassus, in the japanese eel, anguilla japonica, compared with the european eel, a. anguilla.	the humoral immune response in the two eel species, anguilla japonica and anguilla anguilla against two fractions of antigens in anguillicola crassus were studied. within species, both eel species showed significantly elevated titres compared with controls when immunized with antigens from anguillicola crassus. in interspecific comparison, anguilla japonica showed significantly elevated titres in comparison with anguilla anguilla. immunization of anguilla anguilla caused a significantly decrease ...	1999	10526415
fucose in alpha(1-6)-linkage regulates proliferation and histogenesis in reaggregated retinal spheroids of the chick embryo.	we have used the lectin from aleuria aurantia (aal) which is highly specific for alpha(1-6)-linked fucose, to examine its effect on chicken retinogenesis in a reaggregation culture system. when dispersed cells of the embryonic chick retina are reaggregated to form histotypic retinospheroids, aal elicits strong inhibition of spheroid growth. the action of aal is specific, since its effect is dose-dependent, saturable, and inhibited by an excess of fucose. fucosidase treatment entirely abolishes r ...	1999	10536033
metallothionein and heavy metals in brown trout (salmo trutta) and european eel (anguilla anguilla): a comparative study.	the levels and the cellular distribution of heavy metals, and the extent by which the metals binds to metallothionein (mt) in brown trout (salmo trutta) and european eel (anguilla anguilla), were analyzed in order to assess the natural conditions of mt and heavy metals in these two fish species. there were no differences in heavy metals and mt concentrations between males and females of brown trout in a nonreproductive status and between adult brown trout individuals. brown trout presented highe ...	1999	10571463
the contribution of a pyrethroid insecticide to the massive eel (anguilla anguilla) devastation, in lake balaton, in 1995.	in the summer of 1995, 30 tonnes of eel (anguilla anguilla) died in lake balaton, hungary. an investigation was carried out to find the causes of this ecocatastrophe. during this investigation, certain biochemical parameters, i.e. the blood sugar level, the acetylcholinesterase (ache, ec 3.1.1.7), lactate dehydrogenase (ldh, ec 1.1.2.3), glutamic-oxaloacetic transaminase (got, ec 2.6.1.1), and glutamic-pyruvic transaminase (gpt, ec 2.6.1.2) activities in the blood serum of the collected survivin ...	1999	10574437
tgf-beta3 exists in bony fish.	a partial nucleotide sequence of transforming growth factor-beta3 (tgf-beta3) has been isolated from the siberian sturgeon (acipenser baeri), rainbow trout (oncorhynchus mykiss) and european eel (anguilla anguilla), confirming a ubiquitous presence in the ray-finned (actinopterygian) bony fish. the bony fish tgf-beta3 is highly conserved, with some 83-84% nucleotide identity (coding region) and 90-95% predicted amino acid identity to known homeotherm tgf-beta3's. far lower homologies are apparen ...	1999	10614492
[electron microscopical and ultrahistochemical investigations of erythrocytes from eel (anguilla angiulla) and rainbow-trout (salmo giardneri) under the specific consideration of the segregation apparatus and the marginal band (author's transl)].	comparative studies were carried out on the fine structure of the erythrocytes of the european eel (anguilla anguilla) and the rainbow trout (salmo gairdneri) using electron microscopy and ultrahistochemistry. the cell organelles, the marginal band and the segregation apparatus were investigated. the 2 organelles revealed specific differences. an alkaline phosphatase activity was revealed by ultrahistochemistry at the cell surface only, while alternating activities between the nuclear membranes, ...	1998	104531
distribution and prevalence of anguillicola crassus (nematoda) in eels anguilla anguilla of the rivers rhine and naab, germany.	over a period of 2 yr eels were collected at several sites along the river rhine as well as from adjacent waters and examined for anguillicola crassus in the swimbladder. additionally eels were collected once from a site on the river naab (danube system). all eels were searched for both juvenile and adult stages of a. crassus, revealing prevalences of 80% in eels from the river rhine and 39% in eels from the river naab. the mean intensities were 5.6 and 7.7, respectively. with respect to the pre ...	1998	9676253
impact of low water temperature on the development of anguillicola crassus in the final host anguilla anguilla.	the effect of low water temperatures on the development and viability of larval and adult anguillicola crassus (nematoda) in the final host anguilla anguilla was studied. european eels were experimentally infected with a. crassus and then maintained for 4 mo at 4, 9, 10, and 19 degrees c. larval development showed a temperature-dependent pattern and was significantly retarded at low temperatures. third-stage larvae survived a 4 mo period at 4 degrees c without being affected, although they were ...	1998	9722403
distribution, characterization, and growth hormone-releasing activity of pituitary adenylate cyclase-activating polypeptide in the european eel, anguilla anguilla.	the complementary dna encoding pituitary adenylate cyclase-activating polypeptide (pacap) has been cloned from two species of teleost fishes, the sockeye salmon and the thai catfish, and the amino acid sequence of pacap has been determined in another teleost, the stargazer. however, to date, the detailed distribution of pacap immunoreactivity has never been investigated in the fish brain. in the present study, we have determined the localization of pacap-immunoreactive neurons in the central ner ...	1998	9751513
redescription of rhabdochona anguillae (nematoda: rhabdochonidae), a parasite of eel, anguilla anguilla, in europe.	the nematode rhabdochona anguillae spaul, 1927, a specific intestinal parasite of the european eel, anguilla anguilla (l.), is redescribed and illustrated from specimens collected from eels of the sousa river, northern portugal (prevalence 20%, intensity 2-13). the species is characterized by the presence of 14 anterior teeth in the prostom, small non-bifurcate deirids, absence of lateral preanal papillae, by the length (0.460-0.660 and 0.130-0.150 mm) and the shape of spicules, fairly large (0. ...	1998	9805785
radiodiagnostic method for studying swimbladder inflammation caused by anguillicola crassus (nematoda: dracunculoidea).	the nematode anguillicola crassus appeared in europe about 15 yr ago, and has subsequently become recognised as a cause of major pathological lesions in the swimbladder of the european eel anguilla anguilla. the radiographic method reported in this paper showed be a useful complement to diagnostic methods that have so far been based exclusively on fish dissection. using this method, the infection of the swimbladder and the severity of its pathological changes can be assessed in a reliable manner ...	1998	9828409
different putative neuromodulators are present in the nerves which distribute to the teleost skeletal muscle.	the presence of putative neuromodulators in the nerve fibres was investigated in white skeletal muscle of two teleost fish not taxonomically correlated and showing different patterns of innervation (multiple versus focal innervation). cryostat sections of epaxial, hypaxial and adductor mandibulae (am) muscles of sparus aurata and anguilla anguilla were stained histochemically for reduced nicotinamide adenine dinucleotide phosphate (nadph)-diaphorase. other sections were used for indirect immunoh ...	1998	9810486
composition and diversity of helminth communities in eels anguilla anguilla in the river tiber: long-term changes and comparison with insular europe	most studies of helminth communities in the european eel anguilla anguilla have been undertaken in the british isles, and there are very few analyses of community composition and structure from continental europe. to fill this gap and test the hypothesis that helminth communities in freshwater eels in the british isles are not typical of those of continental europe, helminth communities of eels in the river tiber below rome were analysed by season using data collected in 1980 and new data from 1 ...	1998	9858625
metabolic studies on eel (anguilla anguilla l.) hepatocytes in primary culture: effect of 17 beta-estradiol and growth hormone.	previous studies demonstrated that native and recombinant growth hormone from mammalian and fish species potentiate the estrogenic induction of vitellogenin synthesis by cultured eel hepatocytes. in the present study, the metabolic competence (respiratory activity and estradiol catabolism) of cultured hepatocytes and their functional capacity to synthesize a specific protein, vitellogenin, in the presence of estradiol and/or bovine growth hormone was investigated. in addition, we examined the po ...	1998	9883567
amphibians and larvae of aquatic insects as new paratenic hosts of anguillicola crassus (nematoda: dracunculoidea), a swimbladder parasite of eels.	amphibians (tadpoles of the frog bombina bombina [l.] and the newt triturus vulgaris [l.]) and aquatic insects (larvae of the alderfly sialis lutaria [l.] [megaloptera], dragonflies sympetrum sanguineum [miller] and coenagrion puella [l.] [odonata], and the caddisfly oligotrichia striati [l.] [trichoptera]) were found to serve as paratenic hosts for the third-stage larvae (l3) of the nematode anguillicola crassus kuwahara, niimi et itagaki, 1974, a pathogenic swimbladder parasite of the eel angu ...	1998	9925427
heterogeneity among isolates of vibrio vulnificus recovered from eels (anguilla anguilla) in denmark.	the findings of this study demonstrate that vibrio vulnificus isolates recovered from diseased eels in denmark are heterogeneous as shown by o serovars, capsule types, ribotyping, phage typing, and plasmid profiling. the study includes 85 v. vulnificus isolates isolated from the gills, intestinal contents, mucus, spleen, and kidneys of eels during five disease outbreaks on two danish eel farms from 1995 to 1997, along with a collection of 12 v. vulnificus reference strains. the results showed th ...	1998	9835548
lectin histochemistry reveals the appearance of m-cells in peyer's patches of scid mice after syngeneic normal bone marrow transplantation.	peyer's patches in the intestinal mucosa are characterized by the presence of several lymphatic follicles and interfollicular t-cell regions. luminal antigens are transported across the intestinal epithelium to stimulate the peyer's patch pre-b-cells in the follicles that proliferate and migrate to distant sites. evidence suggests that antigen priming of b-lymphocytes is responsible for the number and location of peyer's patches during postnatal life, but little is known about the histogenesis o ...	1998	9446820
in vivo inhibition of ache activity in the european eel anguilla anguilla exposed to technical grade fenitrothion.	european eel (anguilla anguilla) were exposed to sublethal fenitrothion concentrations in a continuous flow-through system for 4 days. plasma acetylcholinesterase (ache) activity was evaluated after 2, 8, 12, 24, 32, 48, 56, 72 and 96 h pesticide exposure. ache activity in the plasma of the eel decreased as concentration of fenitrothion increased. pesticide induced significant inhibitory effects on the ache activity of a. anguilla ranging from 51% inhibition at sublethal concentration of 0.02 pp ...	1998	9827055
cu uptake, metabolism and elimination in fed and starved european eels (anguilla anguilla) during adaptation to water-borne cu exposure.	64cu accumulation and total cu concentrations were measured in gill filaments, plasma, liver and bile of fed and starved european eels (anguilla anguilla) during 28 days of exposure to 12 and 94 micrograms cu l-1. branchial cu uptake was found to be 0.03 and 0.13 microgram cu g gill filament-1 h-1 at 12 and 94 micrograms cu l-1, respectively, throughout 28 days of cu exposure. at least during exposure to 94 micrograms cu l-1, the basolateral membrane seemed to be the rate limiting step for branc ...	1998	9827044
dopamine receptor subtypes expressed in vertebrate (carp and eel) retinae: cloning, sequencing and comparison of five d1-like and three d2-like receptors.	eight dopamine receptor-like cdna clones were isolated from the carp (cyprinus carpio) retina and four dopamine receptor-like cdna clones were isolated from the european eel (anguilla anguilla) retina. these cdna clones show high sequence and structural homology to the known dopamine receptor subtypes. the sequence similarity and phylogenetic analysis revealed that five subtypes (d1a3, d1a4, d1b, d1c and d1x) in the carp retina and four subtypes (d1a1, d1a2, d1b and d1c) in the eel retina are d1 ...	1998	9826915
pesticide toxicokinetics in fish: accumulation and elimination.	bioaccumulation of fenitrothion in the european eel (anguilla anguilla) was studied using two sublethal concentrations of this pesticide in a flow-through test system. the pesticide concentrations used were one-tenth (0.002 ppm) and one-fifth (0.04 ppm) the 96-h lc50 of fenitrothion in this species. steady state was reached early (2 h) when the animals were exposed to 0.02 ppm of toxicant, and after 48 h when the animals were exposed to 0.04 ppm. toxicokinetic parameters for fenitrothion in eel ...	1998	9799575
insulin-like growth factor-i stimulates gonadotrophin production from eel pituitary cells: a possible metabolic signal for induction of puberty.	insulin-like growth factor (igf)-i has been suggested as a potential signal linking growth and puberty in mammals. using the juvenile european eel as a model, we employed a long-term, serum-free primary culture of pituitary cells to study the direct effect of igf-i on gonadotrophin (gth-ii=lh) production. igf-i increased both cell content and release of gth-ii in a time- and dose-dependent manner. igf-i and igf-ii had similar potencies but insulin was 100-fold less effective, suggesting the impl ...	1998	9795340
adenylyl cyclase activity and glucose release from the liver of the european eel, anguilla anguilla.	the properties of adenylyl cyclase (ac) in liver membranes of the european eel (anguilla anguilla) and the involvement of camp in glucose release from isolated hepatocytes in response to catecholamines were studied. basal enzyme activity seemed essentially unaffected by gtp, while a biphasic response to increasing nucleotide concentrations was obtained in the presence of epinephrine. eel liver ac was dose-dependently stimulated by guanosine 5'-o-(3-thiotriphosphate) and inhibited by guanosine 5' ...	1998	9791074
genetic variation of the european eel (anguilla anguilla)	the genetic population structure of the european eel anguilla anguilla l. was investigated by sequencing the mitochondrial d-loop region of 55 eels caught at different european locations. in total, 51 haplotypes were identified. pairwise genetic distances ranged from 0% to 6.33% with an average value +/- sd of 3.01% +/- 1.18%, indicating little dna differentiation among the european eel population. none of the node bifurcations of the neighbor-joining phylogenetic tree was strongly supported, su ...	1998	9892716
liver energy metabolism of anguilla anguilla after exposure to fenitrothion.	this paper deals with the effect of fenitrothion (0.04 mg/liter) on the energy metabolism of the european eel, anguilla anguilla, and its recovery from intoxication. various parameters such as glycogen, lactate, proteins, total lipids, and glucose in eel liver and blood were analyzed after 2, 8, 12, 24, 32, 48, 56, 72, and 96 h of fenitrothion exposure. subsequently, the fish were allowed recovery periods of 8, 12, 24, 48, 72, 96, 144, and 192 h in clean water, and the same parameters were evalu ...	1998	9756704
distribution, characterization, and growth hormone-releasing activity of pituitary adenylate cyclase-activating polypeptide in the european eel, anguilla anguilla* * this work was supported by grants from inserm u-413, the conseil supérieur de la pêche, and the conseil régional de haute-normandie.		1998	28200229
heterogeneity of eel thyrotropin beta mrnas is due to a minisatellite in the 3' untranslated region of the gene.	the aim of this study was to determine the causes of the high heterogeneity, in the number and the length, of the thyrotropin (tsh) beta mrna in the european eel. northern blot analysis showed that removal of the poly(a) tail did not affect this heterogeneity. pcr amplification on reverse-transcribed pituitary rnas (rt) showed the main source of heterogeneity to be a highly variable region in the 3' untranslated region (utr). pcr amplification of the 3' utr from rts and genomic dnas demonstrated ...	1998	9714823
hydrogen ion titrations of the anodic and cathodic haemoglobin components of the european eel anguilla anguilla.	h+ titrations were conducted on the separated haemoglobin components of eel anguilla anguilla in both the oxygenated and deoxygenated states. in anodic haemoglobin, the addition of gtp, and to a lesser extent c1-, increased the magnitude of the haldane effect and shifted its maximum value into the in vivo ph range. of the 22 histidine residues in the anodic component, only approximately seven were titratable, presumably the beta-chain residues at positions 41, 97, 109 and 146 (helical positions ...	1998	9698585
tyrosine hydroxylase in the european eel (anguilla anguilla): cdna cloning, brain distribution, and phylogenetic analysis.	we report the isolation of a full-length eel tyrosine hydroxylase (th) cdna that is characterized by a long 3' untranslated region and by a diversity restricted to the 3' end owing to the differential use of three polyadenylation signals. the longest eel th mrna was distinctive in the presence of four pentameric elements (auuua) in the au-rich 3' noncoding region. such a diversity could provide the basis of posttranscriptional or translational regulation of eel th gene expression. comparison of ...	1998	9681435
long-term inhibitory effects of somatostatin and insulin-like growth factor 1 on growth hormone release by serum-free primary culture of pituitary cells from european eel (anguilla anguilla).	to investigate the ability of hypothalamic and peripheral factors to directly regulate growth hormone (gh) release in a primitive teleost, the european eel (anguilla anguilla l.), we used primary cultures of dispersed pituitary cells. when cultured for 12 days in a serum-free medium, pituitary cells continuously released large amounts of gh, which exceeded the initial cellular content. somatotropin-release inhibiting hormone (srih-14) dose-dependently inhibited gh release (ec50 0.75 nm) up to a ...	1998	9641611
switch in rod opsin gene expression in the european eel, anguilla anguilla (l.).	the rod photoreceptors of the european eel, anguilla anguilla (l.), alter their wavelength of maximum sensitivity (lambda max) from c.a. 523 nm to c.a. 482 nm at maturation, a switch involving the synthesis of a new visual pigment protein (opsin) that is inserted into the outer segments of existing rods. we artificially induced the switch in rod opsin production by the administration of hormones, and monitored the switch at the level of mrna accumulation using radiolabelled oligonuleotides that ...	1998	9633112
sublethal bioconcentration of fenitrothion in the blood and brain of the european eel.		1998	9595200
studies on the eel sperm flagellum. 2. the kinematics of normal motility.	the sperm flagellum of anguilla anguilla lacks outer dynein arms, radial spokes and central structures. its characteristic motion has been obtained by studying cells swimming perpendicularly against, but not adhering to, the coverslip. the flagellum generates a sinistrally helical wave of rising, then falling, amplitude. the frequency of the wave, which can exceed 70 hz, is inversely related to its maximum amplitude. as a reaction to the torque, the entire cell rolls (spins) in the opposite dire ...	1998	9519904
uptake and elimination kinetics of a pesticide in the liver of the european eel.	this study was undertaken to determine the bioconcentration-elimination process of fenitrothion in the liver of the european eel (anguilla anguilla). the animals were exposed to two sublethal fenitrothion concentrations corresponding to 1/5 and 1/10 of lc50 96-h in a flow-through test system. uptake kinetics were determined from liver burdens measured at 2, 8, 24, 48, 56, 72 and 96 hours exposure. after pesticide exposure, animals were transferred to clean water for 72 hours. fenitrothion elimin ...	1998	9491569
localization of pituitary adenylate cyclase-activating polypeptide in the central nervous system of the european eel anguilla anguilla: stimulatory effect of pacap on gh secretion.		1998	9928053
induction of erod activity and genotoxic effects by polycyclic aromatic hydrocarbons and resin acids on the juvenile eel (anguilla anguilla l.).	the purpose of this research is the study of total ethoxyresorufin o-deethylase (erod) activity and erythrocytic nuclear abnormalities (na) induction by polycyclic aromatic hydrocarbons (pahs) and resin acids (ras) on the juvenile eel anguilla anguilla l. juvenile eels were exposed, during 3 days, to the same molar concentration range (0, 0.1, 0.3, 0.9, and 2.7 microm) of beta-naphthoflavone (bnf) and benzo[a]pyrene (bp)--pahs--and abietic acid (aa) and dehydroabietic acid (dhaa)--ras--and one a ...	1997	9469877
response and recovery of brain acetylcholinesterase activity in the european eel, anguilla anguilla, exposed to fenitrothion.	european eel (anguilla anguilla) were exposed to sublethal fenitrothion concentrations in a continuous flow-through system for 4 days. brain acetylcholinesterase (ache) activity was evaluated after 2, 8, 12, 24, 32, 48, 56, 72, and 96 h pesticide exposure. results indicated that ache activity in eel brains decreased as the concentration of fenitrothion increased. the pesticide induced significant inhibitory effects on the ache activity of a. anguilla, ranging from > 40% inhibition at a sublethal ...	1997	9469870
inhibition of gill na+,k+-atpase activity in the eel, anguilla anguilla, by fenitrothion.	european eels (anguilla anguilla) were exposed to sublethal fenitrothion concentrations (0.02 and 0.04 mg/liter) in a continuous flow-through system for 4 days. gill mg2+- and na+,k+-atpase activities were evaluated after 2, 8, 12, 24, 32, 48, 56, 72, and 96 h of pesticide exposure. results indicated that atpase activity in gill tissue decreased as concentration of fenitrothion increased. pesticide induced significant inhibitory effects on the na+, k+-atpase activity of a. anguilla, ranging from ...	1997	9417854
effects of vagal stimulation on swimbladder blood flow in the european eel anguilla anguilla.	the influence of the vagus nerve on swimbladder blood flow in the european eel (anguilla anguilla) was characterized by recording the changes in blood flow rate and blood pressure following stimulation of the vagus nerve. after electrical stimulation, blood flow in the swimbladder artery increased from 0.9 ml min-1 to 2.1 ml min-1. video recordings of small vessels on the caudal side of the rete mirabile revealed an increase in erythrocyte velocity combined with a small vasodilation. this effect ...	1997	9364021
ph-dependent proton secretion in cultured swim bladder gas gland cells.	the ph dependence of acid production and of acid release has been analyzed in cultured gas gland cells of the european eel using a cytosensor microphysiometer. total acid release of gas gland cells showed an optimum at ph 7.4-7.6, with only a minor reduction at acidic (ph 7.0) as well as at alkaline ph (ph 8.0). the acid production was largely dependent on the availability of extracellular glucose and was almost completely abolished if glucose was replaced by succinate, alanine, or even pyruvate ...	1997	9374815
response and recovery of acethylcholinesterase activity in the european eel anguilla anguilla exposed to fenitrothion.	european eel (anguilla anguilla) were exposed to sublethal fenitrothion concentrations in a continuous flow-through system for 4 days. muscle acetylcholinesterase (ache) activity was evaluated after 2, 8, 12, 24, 32, 48, 56, 72 and 96 hours pesticide exposure. results showed that ache activity in eel muscle tissue decreased as concentration of fenitrothion increased. pesticide induced significant inhibitory effects on the ache activity of a. anguilla ranging from > 35% inhibition at sublethal co ...	1997	9350079
the anodic hemoglobin of anguilla anguilla. molecular basis for allosteric effects in a root-effect hemoglobin.	the functional and structural basis for the root effect has been investigated in the anodic hemoglobin of the european eel, anguilla anguilla. this hemoglobin exhibits a large bohr effect, which is accounted for by oxygen-linked binding of seven to eight protons in the presence of gtp at ph 7.5. oxygen equilibrium curves show nonlinear lower asymptote of hill plots, indicating the occurrence of heme-heme interactions within the t state. analysis of the curves according to the co-operon model (br ...	1997	9188451
an electroneutral anion exchange mechanism is present in brush-border membranes isolated from eel kidney.	the mechanism of bicarbonate translocation across the luminal membrane of the eel (anguilla anguilla) kidney tubular cells was studied by monitoring the uptake of h14co3- into isolated brush-border membrane vesicles. results indicate that the presence of a transmembrane outwardly directed cl- gradient was able to transiently accumulate h14co3- into the vesicular space, whereas neither an inwardly directed sodium gradient nor a transmembrane electrical potential gradient (inside positive) was abl ...	1997	9140013
cdna cloning of eel (anguilla anguilla) somatolactin.	the nucleotide sequence of the cdna coding for eel somatolactin (sl), a pituitary hormone belonging to the growth hormone (gh)/prolactin (prl) family, has been determined. the full-length eel sl cdna (1213 bp) encodes a protein of 229 amino acids (aa), with a putative signal peptide of 24 aa and a mature protein of 204 aa. eel sl contains seven cys residues found to be characteristic of sls, and two potential n-glycosylation sites. significant sequence homology between eel and seven other fish s ...	1997	9099860
residue study of mebendazole and its metabolites hydroxy-mebendazole and amino-mebendazole in eel (anguilla anguilla) after bath treatment.	mebendazole (mbz) is extensively used in eel culture for treatment of pseudodactylogyrus spp. infections. this use may lead to residues of mbz in eel tissues. consequently, the residue profile of mbz in eel after treatment with the drug is of special concern. therefore, a residue study was performed in european eels (anguilla anguilla), bath-treated with mbz at a dose of 1 mg/liter for 24 hr and kept at a water temperature of 25 degrees c. liver, kidney, fat, skin, and muscle tissues samples wer ...	1997	9172949
angiotensin ii receptors in the gill of sea water- and freshwater-adapted eel.	immunocytochemistry of paraffin-embedded and cryostat sections of eel (anguilla anguilla) gill showed that angiotensin ii receptors (ang ii-r) were present in chloride cells, uniformly distributed in the cytoplasm and on surface membranes. computerised image analysis of these preparations showed that gills from sea water (sw)-adapted animals had a significantly (3-fold) higher ang ii-r concentration compared with freshwater (fw)-adapted eel gills. isoelectric focusing gel electrophoresis reveale ...	1997	9061608
sublethal effects of an organophosphate insecticide on the european eel, anguilla anguilla.	the present communication deals with the effects of fenitrothion (0.02 mg/liter) on the energy metabolism of the european eel, anguilla anguilla, and its recovery from intoxication. various parameters such as glycogen, lactate, proteins, and glucose levels were measured in different eel tissues after 2, 8, 12, 24, 32, 48, 56, 72, and 96 hr of fenitrothion exposure. subsequently, the fish were allowed recovery periods of 8, 12, 24, 48, 72, 96, 144, and 192 hr in clean water, and the same paramete ...	1997	9056401
histopathological changes induced by environmental stress in common carp, japanese coloured carp, european eel, and african catfish.	histopathological changes caused by stress during catching and transport were examined in four fish species, the common carp (cyprinus carpio), the japanese coloured carp (koi, the coloured variant of the asian carp, cyprinus carpio haematopterus), the european eel (anguilla anguilla), and the african catfish (clarias gariepinus). in all species, the goblet cells of the gills and skin were decreased in number and slight detachment of the epithelium of the secondary gill lamellae was seen. loss o ...	1997	9270124
changes in the electrophysiological parameters of the posterior intestine of anguilla anguilla (pisces) induced by oxytocin, urotensin ii and aldosterone.	in view of the importance of the intestine in the osmoregulation of freshwater fishes, we determined the effects of oxytocin, urotensin ii (uii), and aldosterone added to the serosal side of the isolated posterior intestine of the freshwater-adapted teleost anguilla anguilla on electrophysiological parameters. oxytocin decreased the short-circuit current (scc) and transepithelial potential difference (tpd) at concentrations of 1 and 10 mu/ml (to 50% and 42% of control values, respectively), but ...	1997	9222401
h(+)-glycyl-l-proline cotransport in brush-border membrane vesicles of eel (anguilla anguilla) intestine.	a plasma membrane h(+)-glycyl-l-proline (gly-l-pro) cotransport mechanism has been identified in isolated eel intestinal brush-border membrane vesicles (bbmv) by both measuring radiolabeled gly-l-pro uptake and monitoring gly-l-pro-dependent h+ influx with the ph-sensitive dye acridine orange. the application of an inside negative membrane potential resulted in increasing gly-l-pro uptake, as well as the application of inwardly directed h+ gradient (although only when an inside negative membrane ...	1997	9039012
androgens stimulate gonadotropin-ii beta-subunit in eel pituitary cells in vitro.	primary cultures of juvenile eel (anguilla anguilla) pituitary cells were used to study the direct effects of sex steroids on gonadotropin (gth-ii) cell content and release (radioimmunoassay) as well as on mrnas levels for alpha and gth-ii beta-subunits (dot-blot). testosterone stimulated gth-ii production in a dose- and time-dependent manner by selectively increasing mrnas for gth-ii beta-subunit but not alpha-subunit. this positive effect was also induced by non-aromatizable androgens (androst ...	1997	9296374
habitat use by the fish assemblages of two chalk streams	patterns of habitat use by fish assemblages in two chalk streams in southern england were examined to identify species preferences with respect to major habitat gradients. both study sites, although differing in some physical habitat characteristics, mainly channel width, water temperature and instream cover, could be arranged similarly along a continuum extending from erosional to depositional habitats. twelve fish species were collected from stream surveys conducted during july 1993. the habit ...	1997	9236089
attempts to analyse anguillicola crassus infection and the humoral host response in eels (anguilla anguilla) of lake balaton, hungary.	since the introduction of anguillicola crassus into europe, anguillicolosis has been a considerable problem in several countries. from 1991, periodical eel mortality occurred in lake balaton, hungary. however, eels with a worm burden of 20 to 50 parasites did not show severe swimbladder lesions, which observation cast doubts on the primary aetiological role of the parasite in the eel kill. in order to study the pathology of the infection, from the spring of 1996 until october of the same year, 5 ...	1997	9557321
early emergence of three dopamine d1 receptor subtypes in vertebrates. molecular phylogenetic, pharmacological, and functional criteria defining d1a, d1b, and d1c receptors in european eel anguilla anguilla.	the existence of dopamine d1c and d1d receptors in xenopus and chicken, respectively, challenged the established duality (d1a and d1b) of the dopamine d1 receptor class in vertebrates. to ascertain the molecular diversity of this gene family in early diverging vertebrates, we isolated four receptor-encoding sequences from the european eel anguilla anguilla. molecular phylogeny assigned two receptor sequences (d1a1 and d1a2) to the d1a subtype, and a third receptor to the d1b subtype. additional ...	1997	9006917
four polymorphic microsatellite markers in the european eel anguilla anguilla (l.).		1997	9124719
composition and structure of helminth communities in eels anguilla anguilla from italian coastal lagoons.	the composition and diversity of total and intestinal helminth component and infracommunities were determined in eels anguilla anguilla from four shallow coastal lagoons near rome. the lagoons differed principally in respect of their salinity. only lake burano still received an input of freshwater, and both freshwater and marine helminth species were found in eels. in the other three lagoons all helminth species were marine or euryhaline and communities were dominated by digeneans. at component ...	1997	9166436
similarities and differences between the massive eel (anguilla anguilla l.) devastations that occurred in lake balaton in 1991 and 1995.	in the past few years, two massive eel (anguilla anguilla l.) devastations occurred in lake balaton, hungary. in 1991, 300 tons of eel perished in the western basin of the lake, while in the summer of 1995 30 tons of eel died in the eastern part of the lake. investigations carried out to find the causes of these ecocatastrophes included measurements of certain biochemical parameters: the blood sugar level, and the acetylcholinesterase (ache, ec 3.1.1.7), lactate dehydrogenase (ldh, ec 1.1.2.3), ...	1997	9212331
endoparasitic helminths of the european eel, anguilla anguilla, from four disconnected meanders from the rivers leie and scheldt in western flanders, belgium.	the endoparasitic helminth communities of the european eel, anguilla anguilla (l.), were investigated in four meanders, cut off from the rivers leie and scheldt in western flanders, belgium. six species of helminths (2 cestodes, 2 nematodes and 2 acanthocephalans) were found. the dominant parasite species was the nematode anguillicola crassus (kuwahara, niimi et itagaki, 1974) infecting 79% of the eel population with intensities up to 112 specimens per fish. at two localities no acanthocephalans ...	1997	9229569
bioconcentration and excretion of fenitrothion in the brain of the eel (anguilla anguilla).	the bioconcentration of fenitrothion in the brain of the european eel (anguilla anguilla) and its posterior elimination have been studied. animals were exposed to a sublethal concentration of fenitrothion (0.04 mg/l) for 96 hours in a flow-through test system. after this pesticide exposure, animals were transferred to clean water for 72 hours more. bioconcentration and elimination processes of fenitrothion were studied in blood and brain. this insecticide showed a strong tendency to bioconcentra ...	1997	9350078
down-regulation of tsh subunit mrna levels by thyroid hormones in the european eel.	the effects of 3,5,3'-triiodothyronine (t3) and thyroxine (t4) on alpha and thyroid-stimulating hormone (tsh) beta subunit mrna pituitary levels were examined in a teleost, the european silver eel. northern blot analysis showed that the number and length of mrnas encoding tsh beta varied among individuals, a variability apparently not related to thyroidal status. when several bands were present, their intensities were summed for quantitative analysis. increasing circulating thyroid hormones (ths ...	1997	9356215
isolation of six putative p-type atpase beta subunit pcr fragments from the brain of the european eel (anguilla anguilla).		1997	9432906
long-term and seasonal changes in composition and richness of intestinal helminth communities in eels anguilla anguilla of an isolated english river.	the need for more long-term studies on helminth communities was addressed by examining changes in composition and diversity of the intestinal helminth component and infracommunities in eels of a small isolated river over 12 years. examination of samples over one summer season indicated that single samples were representative of community richness in that year. in 1985 the community was species poor (1 species only) and with zero diversity, but by 1996 it comprised six species and all parameters ...	1997	9437839
glutathione-s-transferase is an important antigen in the eel nematodeanguillicola crassus	different organs and secretions/excretions of the swimbladder parasite, anguillicola crassus (nematoda), were tested for the presence of antigens to the humoral immune response previously detected in the european eel, anguilla anguilla. proteins from different fractions of anguillicola crassus were separated using sds-page (sodium-dodecyl-sulphate polyacrylamide-gel electrophoresis) under reducing conditions and electroblotted onto nitrocellulose membranes. infected eels showed a specific antibo ...	1997	9443950
polychlorinated biphenyls and organochlorine pesticides in eels (anguilla anguilla l.) from welsh rivers.	organochlorine concentrations in the muscle tissues of eels at 41 freshwater sites across wales were surveyed in 1993. positive but weak relationships were detected between eel age or length and the concentration of some organochlorines. isomers of hch were below detection at most sites. dieldrin was widespread at 10-100 microg kt(-1) wet weight, despite its ban in sheep dip in 1989. total ddt residue concentrations averaged 73 microg kg(-1). total pcb burdens expressed as arochlor 1260, were > ...	1997	15093481
importation of aquaculture seedlings to japan.	japan is one of the largest consumers of fishery products in the world. importation of various fishery commodities - including larvae and eggs for aquaculture - is increasing due to high consumer demand. elvers of anguilla anguilla have been imported from europe since the late 1960s. eyed eggs of coho salmon (oncorhynchus kisutch) are imported from north america. during the last decade, over-production caused the price of japanese amberjack (or 'yellowtail') (seriola quinqueradiata) and japanese ...	1996	8890372
primary cultures of dispersed pituitary cells from estradiol-pretreated female silver eels (anguilla anguilla l.): immunocytochemical characterization of gonadotropic cells and stimulation of gonadotropin release.	to analyze the multihormonal control mechanisms of gth secretion in the eel, primary culture of pituitary cells from control or estradiol-treated female silver eels, a treatment known to stimulate in vivo gth synthesis, was developed. dispersed eel pituitary cells obtained by enzymatic (trypsin/dnase) and mechanical dispersion were cultured in earles m199, at 18 degrees. immunoreactive gth (ir-gth) cells were characterized by the immunoperoxidase method, using antiserum to carp gth beta subunit. ...	1996	8921361
production of recombinant insulin-like growth factor-ii in the development of a radioimmunoassay in rainbow trout (oncorhynchus mykiss).	rainbow trout (oncorhynchus mykiss) insulin-like growth factor-ii (igf-ii) cdna was amplified by reverse transcription-polymerase chain reaction and cloned into an expression vector (parhs3) for production by escherichia coli. recombinant rainbow trout igf-ii (rtigf-ii) was recovered from bacterial inclusion bodies, solubilized, and purified by gel filtration chromatography under reducing conditions. after refolding of the peptide by dialysis at basic ph, we obtained a final yield of 820 microgr ...	1996	8930606
colonization and establishment of pomphorhynchus laevis (acanthocephala) in an isolated english river.	the successful colonization, establishment and spread of pomphorhynchus laevis in a small, isolated, devon river 128 km from the parasite's nearest focus in dorset was followed over 11 years from 1985. the parasite was first detected in anguilla anguilla and platichthys flesus in 1988: by 1995 it had attained prevalence levels of 22.6% in a. anguilla and 43.6% in p. flesus and also occurred in 100% salmo trutta, 50% cottus gobio and noemacheilus barbatulus. as judged by prevalence, abundance, pr ...	1996	8991914
potentiating effect of growth hormone on vitellogenin synthesis induced by 17 beta-estradiol in primary culture of female silver eel (anguilla anguilla l.) hepatocytes.	previous in vivo experiments have indicated a potentiating role of growth hormone (gh) during experimentally induced vitellogenesis by 17-beta-estradiol (e2) in the female silver eel (anguilla anguilla l.). to investigate whether gh has direct hepatic actions, the effects of hypophysial-purified and recombinant qh on vitellogenin (vg) synthesis in response to e2 were tested on primary cultures of hepatocytes. hepatocytes were prepared from control or e2-primed eels. addition of e2 alone into the ...	1996	8998971
eel (anguilla anguilla) and brown trout (salmo trutta) target species to assess the biological impact of trace metal pollution in freshwater ecosystems.	copper, lead, and cadmium pollution were measured in water and sediments of two northern spanish rivers: piles and pigäeña. liver contents of these heavy metals were analyzed in two fish species (eel, anguilla anguilla, and brown trout, salmo trutta) collected from the same locations. significant levels of heavy metal pollution were found in a 38% of fish that are potential catches for sport fishermen. the results indicate that adult eel could be a good metal bioindicator if sampled at a homogen ...	1996	8854823
localization of rdna genes in european eel (anguilla anguilla) by fish.	a probe containing most of the transcription unit of the major ribosomal genes of xenopus laevis has been used to localize the rdna cluster of european eel (anguilla anguilla) by fluorescence in situ hybridization (fish). only on nor-bearing chromosome pair has been found. the results obtained with this technique confirm the size polymorphism of the nor revealed previously by other classical cytogenetic techniques.	1996	8983192
different effects of cgmp and camp in the intestine of the european eel, anguilla anguilla.	the regulation of salt absorption in the sea water eel intestine was studied by evaluating the effects of theophylline, 8 br cyclic adenosine monophosphate, 8 br cyclic guanosine monophosphate, atriopeptin iii, porcine vasoactive intestinal peptide and prostaglandin e1 on the short-circuit current, the transepithelial voltage difference and conductance and on the dilution potentials. it was shown that theophylline increased the transepithelial conductance and reduced the magnitude of the dilutio ...	1996	8621839
fluorescence-labeled fucolectins are superior markers for flow cytometric quantitation of the human sperm acrosome reaction.	to evaluate the binding of three fluorescein (fitc)-labeled fucose-specific lectins, anguilla anguilla agglutinin (aaa), tetragonolobus purpureas agglutinin (tpa), and ulex europaeus-1 agglutinin (uea-1), to unfixed, acrosome-intact and acrosome-reacted (ar) human sperm by flow cytometry and to compare the results with those found using fitc-labeled pisum sativum agglutinin (psa) and arachis hypogaea agglutinin (pna).	1996	8654649
angiotensin ii stimulation of the basolateral located na+/h+ antiporter in eel (anguilla anguilla) enterocytes.	the ph-sensitive fluorescent dye, 2',7'-bis-carboxyethyl-5, 6-carboxyfluorescein acetoxymethyl ester, was used to examine the effects of fish or human angiotensin ii (ang ii) on the activity of the basolateral located na+/h+ antiporter in eel intestinal cell suspensions. exposure of eel enterocytes to either hormone led to an increased activity of the antiporter. this time- and dose-dependent stimulatory effect was inhibited by the specific antiporter inhibitor dimethylamiloride (dma). preincuba ...	1996	8672233
purification and characterization of the tricarboxylate carrier from eel liver mitochondria.	the tricarboxylate carrier from eel (anguilla anguilla) liver mitochondria was solubilized with triton x-100 and purified by sequential chromatography on hydroxyapatite and matrex gel blue b. on sds-polyacrylamide gel electrophoresis, the purified fraction showed a single polypeptide band with an apparent molecular mass of 30.4 kda. when reconstituted into liposomes, the tricarboxylate transport protein catalyzed a very active 1,2,3-benzenetricarboxylate-sensitive citrate/citrate exchange. it wa ...	1996	8687426
genomic variation of aquatic birnaviruses analyzed with restriction fragment length polymorphisms.	aquatic birnaviruses are the most ubiquitous and diverse group of viruses in the family birnaviridae. several cause different diseases in a variety of fish species, such as infectious pancreatic necrosis virus in salmonids in north america, europe, and asia and european eel virus in eel in asia. most isolates are antigenically related and belong to a single serogroup (serogroup a) comprising nine serotypes. previous studies with monoclonal antibodies have demonstrated considerable variation in e ...	1996	8779591
development of a radioimmunoassay for european eel growth hormone and application to the study of silvering and experimental fasting.	a specific and sensitive homologous radioimmunoassay for eel (anguilla anguilla l.) growth hormone (anggh) has been developed. the antiserum, raised against purified anggh and used at 1:20,000 final dilution, did not cross-react with eel prolactin or thyrotropin, carp gonadotropin ii, bovine gh, or serum from hypophysectornized eel. the inhibition curves for eel pituitary extracts and serum were parallel to that of anggh standard. the ed50 value was between 1 and 2 ng/tube and the recovery of pu ...	1996	8804566