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fluorescence-labeled fucolectins are superior markers for flow cytometric quantitation of the human sperm acrosome reaction.to evaluate the binding of three fluorescein (fitc)-labeled fucose-specific lectins, anguilla anguilla agglutinin (aaa), tetragonolobus purpureas agglutinin (tpa), and ulex europaeus-1 agglutinin (uea-1), to unfixed, acrosome-intact and acrosome-reacted (ar) human sperm by flow cytometry and to compare the results with those found using fitc-labeled pisum sativum agglutinin (psa) and arachis hypogaea agglutinin (pna).19968654649
angiotensin ii stimulation of the basolateral located na+/h+ antiporter in eel (anguilla anguilla) enterocytes.the ph-sensitive fluorescent dye, 2',7'-bis-carboxyethyl-5, 6-carboxyfluorescein acetoxymethyl ester, was used to examine the effects of fish or human angiotensin ii (ang ii) on the activity of the basolateral located na+/h+ antiporter in eel intestinal cell suspensions. exposure of eel enterocytes to either hormone led to an increased activity of the antiporter. this time- and dose-dependent stimulatory effect was inhibited by the specific antiporter inhibitor dimethylamiloride (dma). preincuba ...19968672233
purification and characterization of the tricarboxylate carrier from eel liver mitochondria.the tricarboxylate carrier from eel (anguilla anguilla) liver mitochondria was solubilized with triton x-100 and purified by sequential chromatography on hydroxyapatite and matrex gel blue b. on sds-polyacrylamide gel electrophoresis, the purified fraction showed a single polypeptide band with an apparent molecular mass of 30.4 kda. when reconstituted into liposomes, the tricarboxylate transport protein catalyzed a very active 1,2,3-benzenetricarboxylate-sensitive citrate/citrate exchange. it wa ...19968687426
effects of diazinon exposure on cholinesterase activity in different tissues of european eel (anguilla anguilla).cholinesterase (che) activity was measured in brain, plasma, and whole eye of anguilla anguilla experimentally exposed to a sublethal concentration of 0.042 mg/liter (0.50 of the 96-hr lc50) of the organophosphorous pesticide diazinon. whole eye was the tissue which revealed higher values of che activity (8.17 micromol/min/g) in nonexposed animals. brain, plasma, and whole eye che activity of a. anguilla was inhibited at 6, 24, 48, 72, and 96 hr of diazinon exposure. pesticide induced significan ...19969006996
anguilla anguilla l. stress biomarkers recovery in clean water and secondary-treated pulp mill effluent.the eels' recovery from capture, anoxia, and transport is improved by the dechlorinated filtered aerated tap water temperature increase and increased water salinity. the eels' 6-hr recovery from capture, anoxia, and transport, at 25 degrees c in 50% secondary-treated bleached kraft pulp mill effluent, as plasma lactate and cortisol uptake, is depressed. however, either farm or wild eels' exposure to the 50% secondary-treated bleached kraft pulp mill effluent, after 188 hr recovery from capture, ...19968930510
a monoclonal antibody to mammalian angiotensin ii at1 receptor recognizes one of the angiotensin ii receptor isoforms expressed by the eel (anguilla anguilla).using labelled ligand-binding methods, previous studies have identified specific angiotensin ii receptors (ang ii-rs) in eel liver, kidney and intestine membranes. isoelectric focusing on polyacrylamide gels also showed that there are two ang ii-r isoforms in eel liver, focusing at isoelectric points (pi) 6.5 and 6.7. these may have different functions. in contrast, eel enterocyte plasma membrane and renal brush border membranes contain only the pi 6.5 form. to characterize the eel receptors mor ...19968672232
control of acid secretion in cultured gas gland cells of the european eel anguilla anguilla.single cells and cell clusters isolated from the swimbladder epithelium of the european eel anguilla anguilla attached to collagen s-coated petri dishes and proliferated in a modified dulbecco's modified eagle's medium, supplemented with 0.5% fetal calf serum. at a temperature of 20-22 degrees c, the growing colonies reached confluence typically within 6-8 days. activities of glycolytic and pentose phosphate shunt enzymes remained stable or increased only slightly during the first 10 days of pri ...19968780223
bicarbonate absorption in eel intestine: evidence for the presence of membrane-bound carbonic anhydrase on the brush border membranes of the enterocyte.bicarbonate absorptive fluxes through the isolated intestine of the european eel (anguilla anguilla) were evaluated by the ph-stat method under short-circuited conditions. it was found that bicarbonate absorptive flux was dependent on the luminal na+ and was inhibited by luminal 4-acetamido-4' stilbene-2-2' disulfonic acid (sits; 2.5 x 10(-4) m) and luminal acetazolamide (10(-4) m), while luminal amiloride (1 mm) was without effect. furthermore, by using brush border membrane vesicles (bbmv) iso ...19968691189
the erythrocyte na+/h+ exchangers of eel (anguilla anguilla) and rainbow trout (oncorhynchus mykiss): a comparative studytrout and eel red blood cell na+/h+ exchangers show widely different regulatory properties. catecholamines, cyclic amp and phorbol esters, which activate the trout red cell antiporter, do not affect the eel exchanger. unlike the trout red cell exchanger, the eel red cell exchanger is strongly activated by cell shrinkage, allowing a remarkable cell volume recovery. these different regulatory properties probably indicate the existence of different isoforms of the exchangers in nucleated erythrocyt ...19969318046
mediation of serotonin-induced hyperventilation via 5-ht3-receptor in european eel anguilla anguilla.the effects of serotonin (5-hydroxytryptamine) on ventilation were investigated by continuous measurements of intrabuccal pressure in unrestrained eel. intravenous administration of 5-hydroxytryptamine (30 micrograms.kg-1) caused a large increase in ventilatory frequency (+ 100%) and amplitude (+ 140%). the 5-hydroxytryptamine-induced hyperventilation was blocked by the 5-ht3-receptor antagonists metoclopramide (1.0 mg.kg-1) or mdl72222 (1.0 mg.kg-1), and was insensitive to the 5-ht1/2-receptor ...19968882510
absorbance spectra and molecular structure of the blue-sensitive rod visual pigment in the conger eel (conger conger).the conger eel (conger conger) is a nocturnal fish that can be found living in shallow coastal water and deep water down to 1000 m. the conger eel has a pure rod retina with a visual pigment maximally sensitive to blue light around 487 nm. we have cloned and sequenced the opsin cdna which is presumed to code for this visual pigment and have found it to be highly homologous to the form of opsin that is expressed in mature, deep-living european eels (anguilla anguilla). the opsin sequence informat ...19968763796
changes in enzyme histochemical profiles of identified spinal motoneurons of the european eel, anguilla anguilla, following cordotomy.the enzyme histochemical profiles of glucose-6-phosphate dehydrogenase (a marker of synthetic performance), succinate dehydrogenase (an indicator of oxidative metabolism), and nadh tetrazolium reductase (a marker of overall neuronal activity) were determined for identified white muscle motoneurons in six control and six cordotomized cels. images were digitized and mean integrated absorbances obtained using appropriate hardware and software. for motoneurons caudal to the transection site there wa ...19968818680
physiological stress responses of anguilla anguilla to fenitrothion.the present communication deals with the effect of fenitrothion (0.04 ppm) on the carbohydrate metabolism of the european eel anguilla anguilla and its recovery from intoxication. analysis of various parameters such as glycogen, lactate and glucose was made on different eel tissues after 2, 8, 12, 24, 32, 48, 56, 72 and 96 hr of fenitrothion exposure. subsequently, the fish were allowed recovery periods of 8, 12, 24, 48, 72, 96, 144 and 192 hr in clean water, and the same parameters were evaluat ...19968847448
swelling-activated k+ transport via two functionally distinct pathways in eel erythrocytes.following osmotic swelling, erythrocytes from the european eel, anguilla anguilla, underwent a regulatory volume decrease. this was prevented by replacement of na+ with k+ in the suspending medium, consistent with a role for the (normally outward) electrochemical k+ gradient in the volume-regulatory response. the effect of cell swelling on k- transport in these cells was investigated using 86rb+ as a tracer for k+. osmotic swelling resulted in an increase in ouabain-insensitive k+ transport that ...19968769785
effects of serotonin on the cardio-circulatory system of the european eel (anguilla anguilla) in vivo.the effects of serotonin on continuously recorded cardiac parameters (heart rate, cardiac output, cardiac stroke volume), ventral and dorsal aortic blood pressures, branchial and systemic vascular resistances were investigated in the european eel in vivo. intravenous administration of serotonin (30 micrograms.kg-1) caused a marked bradycardia (45%) and a simultaneous decrease in cardiac output (50%), ventral (35%) and dorsal (50%) aortic blood pressures. branchial resistance was markedly increas ...19968766910
chromosomal localization of the major and 5s rrna genes in the european eel (anguilla anguilla).the major (18s, 5.8s, and 28s) and 5s rrna genes have been mapped by double fluorescent in situ hybridization to european eel metaphase chromosomes. the major rrna genes were localized to a submetacentric pair of chromosomes that showed a consistent size polymorphism among the individuals studied. the 5s rrna genes were clustered in a single locus that mapped to the centromeric region of an acrocentric pair. in contrast to the major rrna genes, no detectable polymorphism, in either size or inten ...19968646885
the molecular basis for the green-blue sensitivity shift in the rod visual pigments of the european eel.when the european eel matures sexually and migrates back to deep sea breeding grounds the visual pigments in its rod photoreceptors change from being maximally sensitive to green light to being maximally sensitive to blue light. in part, this change in sensitivity is due to a change in the opsin component of the visual pigment molecule. we used hormone injection to induce these developmental changes in a group of eels and from these animals an opsin coding region was cloned and sequenced using c ...19958587887
colocalization of (ttaggg)n telomeric sequences and ribosomal genes in atlantic eels.the distribution of (ttaggg)n telomeric repeats was studied in chromosomes of two atlantic eels, anguilla anguilla and a. rostrata. we found that these sequences hybridize to all the telomeres but also to the entire nucleolar organizer region (nor) localized in both species at the short arm of chromosome 8. this was considered to be due to the interspersion of telomeric sequences within the nor ones. whatever the significance of this interspersion may be, it seems to be limited to a. anguilla an ...19957704417
[anguilluliasis of the european eel--a continuing problem]. 19957571636
effect of hco-3 and ph on ion transport in the posterior intestine of the freshwater-adapted teleost anguilla anguilla.the intestinal mucosa may be exposed to acidic or alcaline solutions due to liberation of digestive secretions. in several situations blood ph may also change. consequently, the effects of hco-3, co2, and ph variation of medium on the ion transport across the posterior intestine of the eel (anguilla anguilla) adapted to freshwater were studied in terms of fractional values of short-circuit current (scc), transepithelial potential difference (tpd) and conductance (g). immature eels weighing 100-2 ...19958520526
production, characterisation and applicability of monoclonal antibodies to european eel (anguilla anguilla l., 1758) immunoglobulin.monoclonal antibodies (mabs) to european eel (anguilla anguilla l., 1758) immunoglobulin (ig) were produced, characterised and tested for applicability in a number of immuno(cyto)chemical assays. the selected mabs, wei 1 and wei 2, were specifically reactive with ig heavy and light chain, respectively. wei 1 appeared to react with all or nearly all ig molecules, b cells and plasma cells. wei 2 was reactive with a subpopulation of those cells, indicating that european eel possesses at least two a ...19957604532
genome size and at content in anguilliformes.the genome sizes (gs) and at contents of five species of anguilliformes (anguilla anguilla, anguilla rostrata, conger conger, gymnothorax unicolor and muraena helena) belonging to three families (anguillidae, congridae and muraenidae) were determined by flow cytometry and densitometry. differences in gs between anguillidae-congridae (about 3 pg) and muraenidae (about 5 pg) were found, which correlate with the relative amounts of at base pairs (around 42-43% for anguillidae-congridae and 51-52% f ...19958835179
characterization of plasma membrane na+/h+ exchange in eel (anguilla anguilla) intestinal epithelial cells.the ability of eel intestinal epithelial cells to recover from an acute acid load was analysed using the fluorescent dye 2',7'-bis-carboxy-ethyl-5,6-carboxyfluorescein (bcecf) and cell suspensions. under these experimental conditions (bicarbonate-free solutions) the resting phi in cells prepared from sea-water (7.52 +/- 0.031) and fresh-water (7.50 +/- 0.094) adapted animals proved to be similar. the recovery rate (following an acid load) increases by increasing the na ion concentration in the e ...19957852945
immunohistochemical localization of bioactive peptides and amines associated with the chromaffin tissue of five species of fish.biogenic peptides and amines associated with the chromaffin tissue in atlantic cod (gadus morhua), rainbow trout (oncorhynchus mykiss), european eel (anguilla anguilla), spiny dogfish (squalus acanthias) and atlantic hagfish (myxine glutinosa) were identified utilizing immunohistochemical techniques. within the posterior cardinal vein (pcv) in cod, trout and eel, a subpopulation of chromaffin cells displayed immunoreactivity to tyrosine hydroxylase (th) and dopamine-beta-hydroxylase (d beta h) b ...19957606765
[effects of hydrostatic pressure on malondialdehyde (mda) determination in brain from yellow freshwater eels].malondialdehyde (mda) content in a tissue can be an indicator of lipoperoxidation and thus of membrane alteration. mda concentrations have been measured in the brain of yellow freshwater eels (anguilla anguilla) submitted to 1.51 or 101 ata of hydrostatic pressure. exposure to pressure was performed for 6 h at constant water temperature and in normoxic conditions. the results thus obtained show a significant increase (p < 0.05 or better) of mda brain concentrations at 51 ata (+ 124%) and 101 ata ...19957583764
effects of a temperature increase on oxygen consumption of yellow freshwater eels exposed to high hydrostatic pressure.oxygen consumption was measured in yellow freshwater eels, anguilla anguilla, during exposure to a hydrostatic pressure (hp) of 101 atmosphere absolute (ata) for 3 h, either with or without a 5 degrees c increase in water temperature. it was found that concomitant increases in temperature and pressure resulted in an increase in oxygen consumption that was lower than when pressure alone was increased. when eels were acclimated to hp for 21 days and then submitted to a temperature increase of 5 de ...19958962704
primary sequence, tissue specificity and mrna expression of the na(+),k (+) -atpase β1 subunit in the european eel (anguilla anguilla).the entire amino acid coding sequence of the na(+),k(+)-atpase β1 isoform was cloned from the gill of the european eel (anguilla anguilla) by a pcr based method. the amino acid sequence translated from the nucleotide sequence shared 61.4 and 56.2% homology respectively with previously published na(+),k(+)-atpase β1 isoform sequences from the clawed toad (xenopus laevis) and the ray (torpedo californica) an elasmobranch fish. the size of the na(+),k(+)-atpase β1 mrna transcript in eel tissues was ...199524197533
the role of the renin-angiotensin system in the control of blood pressure and drinking in the european eel, anguilla anguilla.the role of the renin-angiotensin system (ras) in the control of blood pressure and drinking was investigated in fresh water (fw)- and seawater (sw)-adapted eels, anguilla anguilla, by comparing the effects of pharmacological manipulation through the use of papaverine (stimulator) and captopril (inhibitor) on the endogenous system. in sw eels basal blood pressure levels were lower (23.3 +/- 0.8 mm hg) with correspondingly higher basal drinking rates (0.51 +/- 0.07 ml/kg/hr) and plasma aii concen ...19958575657
mechanisms of acid release in isolated gas gland cells of the european eel anguilla anguilla.mechanisms of acid production and of acid release have been analyzed in isolated gas gland cells of the eel swimbladder using a cytosensor microphysiometer. incubation of isolated cells with oxamic acid caused a dose-dependent decrease in the rate of proton release. at the highest oxamic acid concentration used (20 mmol/l), proton release was reduced by approximately 40%; incubation with sodium fluoride (10 mmol/l) or removal of glucose from the extracellular medium caused 60 and 80% reduction, ...19957485595
influence of flumequine on in vivo mitogen responses of european eel (anguilla anguilla l., 1758) lymphoid cells.the influence of flumequine on mitogen induced lymphoid cell proliferation in european eels (anguilla anguilla l., 1758) was studied. for this purpose an in vivo test, using peroral drug administration followed by successive intraperitoneal injections with concanavalin a (cona) or bacterial lipopolysaccharides (lps) and 5-bromo-2'-deoxyuridine, was applied. direct counting of proliferated cells in blood smears revealed that flumequine possesses mitogenic properties. a synergistic and an antagoni ...19958533292
pesticide effects on eel metabolism pesticide; levels did not decline at any time when animals were exposed to 4.1 mg/liter.previous works on endosulfan eel toxicology in this laboratory demonstrated that 0.041 mg/liter of endosulfan was the 50% lethal concentration of 96 hr exposure. eels of species anguilla anguilla were exposed to two sublethal endosulfan concentrations: 8.2 micrograms/liter (1/5 lc50) and 4.1 micrograms/liter (1/10 lc50), and the experiment was done at different exposure times: 12, 24, 48, 72, and 96 hr. muscle glycogen content decreased significantly (p < 0.05) at 24, 48, 72, and 96 hr exposure ...19958521781
the cathodic hemoglobin of anguilla anguilla. amino acid sequence and oxygen equilibria of a reverse bohr effect hemoglobin with high oxygen affinity and high phosphate sensitivity.as in other fish, the cathodic hemoglobin of the eel anguilla anguilla is considered to play an important role in oxygen transport under hypoxic and acidotic conditions. in the absence of phosphates this hemoglobin shows a reverse bohr effect and high oxygen affinity, which is strongly modulated over a side ph range by gtp (whose concentration in the red blood cells varies with ambient oxygen availability). gtp obliterates the reverse bohr effects in the cathodic hemoglobin. the molecular basis ...19957642546
dynamics of anguillicola crassus (nematoda: dracunculoidea) larval infection in paratenic host fishes of lake balaton, hungary.anguillicola crassus, a nematode parasitizing the swimbladder of the european eel (anguilla anguilla) caused substantial mortality among eels in lake balaton in the years 1991 and 1992. parallel to a 3-year study of the infection dynamics of eels, anguillicola larval infection of paratenic host fishes was also surveyed in the lake between 1991 and 1993. during that study, a total of 1,382 specimens of 22 paratenic host fish species were processed. the results showed that anguillicolosis had beco ...19958882739
primary sequence, tissue specificity and expression of the na+,k(+)-atpase alpha 1 subunit in the european eel (anguilla anguilla).the entire cdna nucleotide sequence of the na+,k(+)-atpase alpha 1 isoform was cloned from the gills of the european eel (anguilla anguilla) by a pcr based method. the amino acid sequence translated from the sequence shared 89.4 and 85.6% homology respectively with previously published na+,k(+)-atpase alpha subunit sequences from elasmobranch (torpedo californica) and teleost (catostomus commersoni) fish. the size of na+,k(+)-atpase alpha 1 mrna transcripts in eel tissues was demonstrated to be ...19958574922
dna dosimetry in biological indicator species living on pah-contaminated soils and sediments.a large variety of environmental carcinogens are metabolically activated to electrophilic metabolites that can bind to nucleic acids, forming covalent adducts. in organisms possessing active metabolic systems for a particular carcinogen, dna adducts generally have longer biological half-lives than the substrate carcinogens. thus, measurement of specific dna adduct concentrations in terrestrial and water organisms may provide a relevant biological indicator of prior exposure to environmental carc ...19957539372
differential regulation of the two forms of gonadotropin-releasing hormone (mgnrh and cgnrh-ii) by sex steroids in the european female silver eel (anguilla anguilla).the effect of steroids on the two gonadotropin-releasing hormone (gnrh) forms present in the eel (mammalian gnrh, mgnrh and chicken gnrh-ii, cgnrh-ii), as well as on gonadotropin (gth), was studied using specific radioimmunoassays. female silver eels received chronic treatments with various steroids (estradiol, testosterone, androstenedione, 5 alpha-androstane-3 beta, 17 beta-diol). estradiol or the combination of estradiol and androgens induced increases in brain and pituitary mgnrh levels and ...19957617130
present occurrence of anguillicola novaezelandiae (nematoda: dracunculoidea) in europe and its development in the intermediate host.an examination of a sample of european eels, anguilla anguilla (l.), collected from lake bracciano near rome in 1993, the only known european locality with the occurrence of the introduced swimbladder nematode anguillicola novaezelandiae moravec et taraschewski, 1988, revealed for the first time the presence of two anguillicola species, a. novaezelandiae and a. crassus. in view of the investigations carried out by current authors in bracciano lake in the years 1982-1992, it is apparent that the ...19947883252
immunocytochemical localization of mammalian gnrh (gonadotropin-releasing hormone) and chicken gnrh-ii in the brain of the european silver eel (anguilla anguilla l.).using specific antibodies for the two molecular forms of gonadotropin-releasing hormone (gnrh) present in the european eel, anguilla anguilla, (mammalian gnrh, mgnrh, and chicken gnrh ii, cgnrh-ii), we employed immunocytochemistry to determine the distribution of these two peptides in the brain and in the pituitary. the results indicate that mgnrh and cgnrh-ii are localized in different neurons: mgnrh-immunoreactive (ir) perikaria were observed in the olfactory bulbs, the junction between olfact ...19947873095
third-stage larvae of daniconema anguillae (nematoda: dracunculoidea) in the subcutaneous tissue of eel anguilla anguilla.daniconema anguillae moravec et køie, 1987 larvae measuring 1.64-1.76 mm were occasionally found in considerable numbers in the fins and subcutaneous connective tissue of approximately 50% of eel anguilla anguilla (l.) sampled from lake balaton, hungary. the larvae were noted for their slender body, very long tail with a rounded tip, a densely transversely striated cuticle, and the presence of boring tooth and large kidney-shaped amphids on the cephalic end. the larvae could easily be recovered ...19947883254
some aspects of zinc transport across eel (anguilla anguilla) red blood cell membranes.zinc movement across eel and human red blood cell membranes was measured by atomic absorption spectrophotometry. it was observed that: 1) in human red blood cells zinc uptake is twice as rapid as in fish red blood cells over a temperature range of 10-40 degrees c. the low rate of zinc uptake in eel red blood cell may be simply the side effect of different surface area to volume ratios for the differences in cell size or, it may be due to the low permeability of bicarbonate through the red blood ...19948044143
two pathways for choline transport in eel erythrocytes: a saturable carrier and a volume-activated channel.choline transport in eel (anguilla anguilla) erythrocytes was investigated in cells suspended in isotonic and hypotonic media. in cells in isosmotic solution choline transport was mediated by a saturable system with a michaelis constant (km; 62 +/- 6 microm) similar to that of the choline carrier of human erythrocytes but a maximal transport rate (vmax; 4.5 +/- 0.4 mmol.1 red blood cells-1.h-1) almost two orders of magnitude higher than that in human red blood cells. this pathway was inhibited b ...19948092322
angiotensin ii receptor subtypes in eel (anguilla anguilla).previous studies have shown the effects of angiotensin ii (ang ii) in teleosts, and ang ii-binding sites have also been localized in tissues from rainbow trout. the purpose of this study was to extend these findings and to provide an analysis of ang ii receptor (ang ii-r) isoforms in three tissues obtained from european eel (anguilla anguilla). ang ii-rs were identified in eel liver, kidney and intestine membranes by the binding of either 0.5 nmol human 125i-labelled tyr4-ile5-ang ii/l or increa ...19948185815
white muscle differentiation in the eel (anguilla anguilla l.): changes in the myosin isoforms pattern and atpase profile during post-metamorphic development.myosin isoforms and their light and heavy chains subunits were studied in the white lateral muscle of the eel during the post metamorphic development, in relation with the myosin atpase profile. at elver stage vi a1 the myosin isoforms pattern was characterized by at least two isoforms, fm3 and fm2. the fast isomyosin type 1 (fm1) appeared during subsequent development. it increased progressively in correlation with the increase in the level of the light chain lc3f. fm1 became predominant at sta ...19941534545
the size and number of neurons descending to the spinal cord in relation to body length in the european eel (anguilla anguilla).cells of origin of descending spinal pathways in eels of different body lengths (15-59 cm) were identified by labelling them retrogradely with horseradish peroxidase from the rostral spinal cord. the aim was to determine whether changes in size, number and distribution of these neurons take place in line with changes in the size of the spinal targets that occur with increasing body length. the somal cross-sectional areas of the labelled cells were measured from horizontal frozen sections and cou ...19948082024
sterol and fatty acid composition of neutral lipids of paratenuisentis ambiguus and its host eel.the sterol composition of free sterol and steryl ester fractions of the fish parasite paratenuisentis ambiguus was determined. in addition, the fatty acid composition of various neutral lipid classes, i.e., wax esters, steryl esters, triacylglycerols and free fatty acids, as well as the composition of the 1-o-alkyl moieties of total ether glycerolipids of the parasite, were investigated. the results of these studies were compared with those obtained on the intestinal tract tissue of its host, th ...19948090063
sequence and regulation of european eel prolactin mrna.cdna clones encoding the european eel (anguilla anguilla l.) prolactin were isolated from a pituitary cdna library constructed in gamma gt10, using a rainbow trout prl cdna fragment as a probe. four different inserts were subcloned into the pgem 3z plasmid after pcr amplification. the 1082 bp-long nucleotide sequence revealed an open reading frame of 627 bp encoding a 24 amino acid-long signal peptide followed by a 185 amino acid-long mature protein. comparison studies showed 60-70% homology wit ...19947926267
in vitro dose-response study of the effect of cadmium on eel (anguilla anguilla) gill na+/k(+)-atpase activities.the effect of cadmium exposure was studied in vitro on the atpase activities of gill membrane microsomes from seawater- and freshwater-adapted eel (anguilla anguilla) using a microassay technique. the basal activity (mg(2+)-atpase) was decreased by 40 and 25%, respectively, in seawater and freshwater preparations for the highest concentrations tested (respectively, 4 and 2 microm). the na+/k(+)-atpase activity was estimated either by potassium stimulation or by ouabain inhibition. this enzyme ac ...19947523067
developmental changes in the content of dopamine in the olfactory bulb of the european eel (anguilla anguilla).the concentrations of dopamine (da) and of its major metabolite, dihydroxyphenylacetic acid (dopac), were measured in discrete areas of the eel brain. to investigate the developmental changes in the content of da and dopac, the assays were performed in yellow eels (i.e. at the feeding stage) and silver eels (i.e. at the migratory stage). da and dopac were unevenly distributed in the eel brain. in yellow eels, the concentration of da was highest (16-19 pmol/mg protein) in the olfactory bulb (ob), ...19948084534
rapid and simultaneous measurement of anodic and cathodic haemoglobins and atp and gtp concentrations in minute quantities of fish bloodoxygen transport to vertebrate tissues is dependent upon (a) the intrinsic o2-binding properties of haemoglobin (hb) and (b) modulation by allosteric effectors, such as nucleoside triphosphates (ntp), that depress hb o2-affinity in fish red cells. while some species, such as flatfish, primarily use adenosine triphosphate (atp) to modulate o2-binding to hb, others, such as eel and carp, also use guanosine triphosphate (gtp) (kono and hashimoto, 1977; leray, 1982; weber and jensen, 1988). unlike m ...19949317794
ion transport in the intestine of anguilla anguilla: gradients and translocatorsthe transport of na+, k+ and cl- across the isolated epithelium of the eel intestine was studied using a combination of four experimental strategies: short-circuiting, measurements of intracellular potentials and ion concentrations, application of a variety of transport inhibitors and measurement of unidirectional fluxes with radioactive tracers. when short-circuited, the system performs a net transport of cl- and na+ towards the blood side, with a stoichiometry approaching 2, and a much smaller ...19949317418
induction of g-bands on anguilla anguilla chromosomes by the restriction endonucleases haeiii, hinfi, and msei.fixed metaphase chromosomes of the eel anguilla anguilla were treated with the restriction enzymes haeiii, hinfi, and msei. all three restriction endonucleases are capable of inducing a g-band-like pattern if the incubation time and enzyme concentration are controlled. the results are discussed in relation to the main factors involved in the appearance of serial g-bands. chromatin organization, rather than dna composition, seems to account for the interstitial g-band pattern exhibited by these e ...19948404070
the distribution of gaba-immunoreactive neurons in the brain of the silver eel (anguilla anguilla l.).the distribution of gaba-immunoreactivity was studied in the brain of the silver eel (anguilla anguilla) by means of antibodies directed against gaba. immunoreactive neuronal somata were distributed throughout the brain. positive perikarya were detected in the internal cellular layer of the olfactory bulb, and in all divisions of the telencephalon, the highest density being observed along the midline. numerous gaba-reactive cell bodies were found in the diencephalon, particularly in the preoptic ...19948192235
an 8-year study on the elimination of pcbs and other organochlorine compounds from eel (anguilla anguilla) under natural conditions. 199422176041
a new mannan-binding lectin from the serum of the eel (anguilla anguilla l.): isolation, characterization and comparison with the fucose-specific serum lectin.a new mannan-binding lectin (mbl) and a previously described fucose-binding lectin (fbl) have been isolated from serum of anguilla anguilla by affinity chromatography on a-peptone-sepharose in combination with electroelution (mbl) or affinity chromatography using alpha-l-fucose-agarose (fbl). mbl has a mol. wt of approximately 246,000 and is composed of identical subunits of approximately 24,000, two of each are always covalently linked. fbl has a mol. wt of about 121,000 and consists of four su ...19947953065
somatostatin-related peptides isolated from the eel gut: effects on ion and water absorption across the intestine of the seawater eel.four somatostatin-related peptides were isolated from eel guts. two of them were the same as eel ss-25ii (ess-25ii) and eel ss-25i (ess-25i) isolated from european eel pancreas. the remaining two peptides were c-terminal tetradecapeptides (ess-14ii and ess-14i) of ess-25ii and ess-25i, respectively. these four peptides all enhanced the serosa-negative transepithelial potential difference and short-circuit current across the seawater eel intestine after pretreatment with isobutylmethylxanthine, s ...19947525832
adrenergic control of swimbladder perfusion in the european eel anguilla anguilla.adrenergic control of swimbladder blood flow was analysed in blood-perfused preparations of the european eel as well as in situ by recording the changes in swimbladder blood flow and blood pressure following an injection of catecholamine into the dorsal artery. in blood-perfused swimbladder preparations, injection of the alpha-adrenergic agonist phenylephrine into the perfusion loop caused a marked dose-dependent increase in perfusion pressure at constant flow, while injection of the beta-agonis ...19947964385
uptake and clearance of diazinon in different tissues of the european eel (anguilla anguilla l.).acute toxicity effects of diazinon on european eel (anguilla anguilla) was examined using short-term exposures in static conditions. the lc50 values were 0.16, 0.11, 0.09 and 0.08 mg/l at 24, 48, 72 and 96 h exposure, respectively. in addition, the eels were exposed to 0.042 mg/l of diazinon and bioaccumulation and clearance processes of this insecticide in liver, muscle and gill tissues were studied. bioconcentration factor (bcf) of diazinon over 96 h exposure period was 1850 in liver, 875 in g ...19948024718
ascorbic acid transport by intestinal brush-border membrane vesicles of the teleost anguilla anguilla.transport of l-ascorbate by intestinal brush-border membrane vesicles of european eel anguilla anguilla was stimulated by a transmembrane na gradient (out > in) but not by a similarly directed gradient of k. under short-circuited membrane potential conditions, a kinetic analysis of l-ascorbate influx indicated the presence of a single na-dependent carrier process (kapp = 0.75 +/- 0.07 mm and jmax = 0.33 +/- 0.03 nmol.mg protein-1.min-1) and a nonsaturable transfer component with an apparent diff ...19938322981
ultrastructural data on the sporogenesis of myxidium giardi, cépède 1906 (myxozoa, myxosporidia), parasite of anguilla anguilla (teleostea).ultrastructural study of myxidium giardi showed that pluricellularity is maintained by the formation of septate desmosomes. the polar capsule is formed from helicoidal depositions of banded material in the initial vacuole. this process makes possible not only the formation of the rigid capsular body but also of the flexible polar filament which is evaginable and reversed in the capsule. the special feature of the genus myxidium is the ornamentation of the valvogenic cells of the spores in the fo ...199323195549
stanniocalcin kinetics in freshwater and seawater european eel (anguilla anguilla).the kinetics and hypocalcemic potency of stanniocalcin (stc) were examined in freshwater and seawater eels. the secretion rate and the metabolic clearance rate of stc were calculated from the stc disappearance curve after intra-arterial injection of trout stc. basal plasma stc concentrations in freshwater and seawater eels did not differ but the stc secretion rate and metabolic clearance rate in seawater eel were 70-75% higher than in fw eel. the increased stc distribution space in seawater eels ...199324214449
peptide hormone-stimulated second messenger production in the teleostean nephron.renal actions of arginine vasotocin (avt) and atrial natriuretic peptide (anp) in teleosts are poorly defined. administration of these hormones changes renal function, largely explained by systemic vascular effects. the present experiments examine the potential direct action of these hormones on the teleost nephron. this has been assessed from the hormones' abilities to stimulate their second messenger systems within isolated nephroi of the rainbow trout onchorhynchus mykiss. following initial e ...19938392960
differential distribution and response to experimental sexual maturation of two forms of brain gonadotropin-releasing hormone (gnrh) in the european eel, anguilla anguilla.using specific radioimmunoassays for the two gnrh molecular forms present in the european eel, anguilla anguilla, (mgnrh and cgnrh ii), we compared their distributions in the pituitary and different parts of the brain of female silver eels, as well as the modifications of their levels in experimentally matured female eels (treated with carp pituitary extract). in control eels, mgnrh levels were higher than cgnrh ii levels in the pituitary, olfactory lobes and telencephalon, di- and mesencephalon ...199324202465
importance of eating habits and sample size in the estimation of environmental mercury contamination using biological indicators.the importance of eating habits and the number of fish sampled in the estimation of mercury environmental contamination through biological indicators was studied. the species used were one with piscivorous habits (anguilla anguilla) and another with omnivorous habits (ciprinus carpio). from one original sample for each species, randomized samples were generated using the subroutine ggsrs from the mathematics library imsl, and later the statistical significance of pearson's correlation coefficien ...199324221024
effect of lipid content of diet on cholesterol content and cholesterogenic enzymes of european eel liver.the effect of dietary lipid levels on the levels of cholesterol and the activities of the major cholesterogenic enzymes of the liver has been studied in the european eel. an increase in hepatic total cholesterol was observed when the dietary lipid levels increased from 12 to 20%, while protein levels were maintained at 30%. this change paralleled an increase in mevalonate 5-pyrophosphate decarboxylase activity, while 3-hydroxy-3-methylglutaryl-coa reductase mevalonate kinase and mevalonate 5-pho ...19938246691
synthesis of vitellogenin by eel (anguilla anguilla l.) hepatocytes in primary culture: requirement of 17 beta-estradiol-priming.control and 17 beta-estradiol-primed eels were used to investigate the hormonal requirement of vitellogenesis in an immature fish, the eel. a primary culture of isolated liver cells from female silver eels was developed. the hepatocytes were maintained as monolayers on poly-l-lysine coated dishes for up to 12 days in a defined medium alone or supplemented with 17 beta-estradiol (e2, from 10(-8) to 10(-5) m). the amounts of vitellogenin (vg) in the cells and secreted into the medium were measured ...19938224775
[cloning and sequence of thyrotropin beta subunit of a teleost fish: the eel (anguilla anguilla l.)].we obtained the sequence of eel thyrotropin beta-subunit cdna. about 1,100 bp long, it encodes a 147-amino acid protein including a 20-residue signal peptide. we analyse homologies between angtsh beta and the other known beta-subunits taking in account the ability of mammal gonadotropins (gth) to stimulate teleost thyroid. a peptide corresponding to mammalian cagyc (implicated in subunit association) is original and different from its eel beta gth2 counterpart.19938044698
the branching of insulin-like growth factor 1 and insulin: an immunohistochemical analysis during phylogeny.the co-existence of insulin-like growth factor 1 (igf-1) with the classical islet hormones insulin (ins), glucagon (gluc), somatostatin (som) and pancreatic polypeptide (pp) in the endocrine pancreas of representative species of cyclostomes (myxine glutinosa), cartilaginous fish (raja clavata, squalus acanthias) and bony fish (cottus scorpius, carassius auratus, cyprinus carpio, anguilla anguilla) was studied by the use of monoclonal and polyclonal antisera and the double immunofluorescence tech ...19938265818
determination of the ca2+ and mg2+ affinity constants of troponin c from eel skeletal muscle and positioning of the single tryptophan in the primary structure.the complete amino acid sequence of troponin c (etnc) from the white muscle of the european eel has been determined by edman degradation procedures. its single tryptophan residue is situated in helix h at amino acid position 152 of the aligned sequence; the tryptophan is the first residue on the c-terminal side of ca2+ binding loop iv. the increase of tryptophan fluorescence emission intensity occurring upon titration of etnc with ca2+ has been used to determine the affinity constants of etnc fo ...19938126218
effect of decreased oxygen content on eels (anguilla anguilla) infected by anguillicola crassus (nematoda: dracunculoidea).the survival chances of eels with anguillicola crassus infection of varying intensity and with varying pathological changes were studied in an experimental system devoid of fresh oxygen supply. eels most severely affected by anguillicolosis died first, while those with less expressed pathological lesions tolerated sublethal oxygen levels for a longer time. findings were similar at 20-21 degrees c and at 27-28 degrees c; at 27-28 degrees c, however, the fish required a higher oxygen content to su ...19938017238
[implication of growth hormone in experimental induction of vitellogenesis by estradiol-17 beta in female silver eel (anguilla anguilla l.)].the effect of different preparations of growth hormone (gh) was assayed with 17 beta-estradiol on vitellogenesis in hypophysectomized or normal female silver eels. vitellogenin (vg) plasma levels were taken as the index of hepatic vitellogenesis. the e2 doses were chosen to give the same pattern for the plasma vg level as in the controls. they decreased or remained undetectable in hypophysectomized or normal animals. gh also failed to induce alone a significant modification. when e2 was injected ...19921638433
affinity purification of antigen-specific serum immunoglobulin from the european eel (anguilla anguilla).immunization of specimens of the european eel with a hapten-carrier conjugate resulted in a significant rise in anti-hapten titres. antigen-specific immunoglobulin was purified on a matrix onto which the hapten-carrier conjugate had been immobilized. rabbit antisera raised against the product of the affinity purification recognized two molecular moieties. the first was eel immunoglobulin as inferred by the polypeptide composition, i.e. disulphide-linked heavy and light chains of 72 kda and 25 kd ...19921615286
a comparison of highly unsaturated fatty acid levels in wild and farmed eels (anguilla anguilla).1. absolute and relative amounts of eicosapentaneoic acid (epa) and docohexaenoic acid (dha) in muscle of eels from four different fish farms were compared with samples from wild eels from two different areas of northern italy. 2. farmed eels were richer in dha and epa than wild animals. 3. the addition of cod liver oil to the diet of farmed eels led to a significant accumulation of epa and dha, but no change in total lipid content. 4. the calculation of two indices related to highly unsaturated ...19921499279
atrial natriuretic peptide in the eel, anguilla anguilla l.: its cardiac distribution, receptors and actions on isolated branchial cells.the presence of atrial natriuretic peptide (anp) and the nature of its binding sites were studied in fresh-water (fw)- and seawater (sw)-adapted eels using a heterologous analogue, that of the rat (ranp). rat anp-like immunoreactivity was demonstrated in the cardiac atria and ventricles of both fw and sw eels, and electron-dense anp-like granules were observed. the atria and ventricles of fw eels contained significantly more granules than those of sw animals and, in both types, the atria were mo ...19921329803
acute toxicity, uptake and clearance of diazinon by the european eel, anguilla anguilla (l.).the toxicity, accumulation, and elimination of diazinon were investigated for the european eel, anguilla anguilla. the 24, 48, 72 and 96-h median lethal concentrations (lc50) were 0.16, 0.11, 0.09 and 0.08 mg/l, respectively. fish exposed to sublethal concentration (0.042 mg/l) accumulated diazinon in liver and muscle tissues. bioconcentration factors (bcf) of diazinon were 1850 in liver, and 775 in muscle over the 96-h exposure period. upon removal from diazinon containing water the contaminate ...19921593096
lindane-induced changes in carbohydrate metabolism in anguilla anguilla.1. anguilla anguilla (l.) was exposed to a sublethal concentration of 0.167 ppm (0.25 of the 96-hr lc50) of lindane for 6, 12, 24, 48, 72 and 96 hr. 2. changes in glycogen, glucose, pyruvate and lactate contents of liver and muscle after lindane exposure, were studied. 3. muscle and liver glycogen levels decreased significantly during the exposure time. muscle glucose values increased but on the other hand we found a decrease in those of liver. 4. muscle and liver pyruvate content increased as d ...19921379899
effect of insulin in the diet on the growth of european eels (anguilla anguilla l.).the effect of oral administration of insulin, in various concentrations, on the growth of european eels (anguilla anguilla l.) was studied. in order to determine whether the insulin penetrated through the stomach or gills to the blood system, 5 ml insulin, suspended in an 0.6% solution of nacl, was inserted via the mouth of eels, and the insulin content in the blood measured by radioimmunoassay immediately, and at one and two hours after administration. a control group was given 0.6% nacl alone. ...199224214275
h+/glycyl-glycine cotransport in eel intestinal brush-border membrane vesicles: studies with the ph-sensitive dye acridine orange.monitoring the fluorescence quenching of the ph-sensitive dye acridine orange, proton accumulation in the presence of an inside-negative transmembrane potential was measured in eel (anguilla anguilla) intestinal brush-border membrane vesicles. it was demonstrated that the proton accumulation was specifically increased by the presence of the dipeptide glycyl-glycine in the extravesicular space, showing saturation kinetics at increasing dipeptide concentrations and was specifically inhibited by di ...19921327139
the use of texture analysis for the discrimination of nissl substance in neurons.structural changes induced by cordotomy in the nissl substance of identifiable spinal motoneurons innervating the white musculature of the european eel were quantified with the use of texture features calculated from digitized images. data were evaluated and the motoneurons classified by using multivariate analysis. the study shows that there are differences in the structural organization of the nissl substance of motoneurons taken from control and cordotomized fishes. distinction could only be ...19921282188
chlorinated hydrocarbons in eels (anguilla anguilla l.) from the river rhine. 19921522922
effects of ambient ion concentrations on gill atpases in fresh water eel,anguilla anguilla.branchial activities of na(+),k(+)-atpase (ouabain sensitive), mg(2+) atpase (ouabain insensitive) and kinetic analysis of high and low affinity ca(2+) atpase were measured inanguilla anguilla that had been acclimated to demineralized water (dw, ca < 10 μm), freshwater (fw, ca = 2 mm), and low calcium freshwater (l-ca, ca = 0.9 mm). na(+),k(+)-atpase activity decreased while ouabain insensitive activity increased when ambient ca(2+) decreased. two kinetic forms of ca(2+) atpase could be resolved ...199224214197
the effect of time on physiological changes in eel anguilla anguilla, induced by lindane.1. eel were exposed to a sublethal concentration of lindane (0.335 ppm) for 6, 12, 24, 48, 72 and 96 hr. 2. concentrations of glycogen, glucose, lactate, pyruvate and lipids were determined in gill tissue after lindane exposure. 3. gill glycogen decreased and glucose levels increased at 6 hr of treatment, lactate and pyruvate concentration increased between 6 and 48 hr. total lipid values decreased between 6 and 24 hr; thereafter, the levels increased up to 72 hr of exposure. 4. clear changes we ...19911713818
significant year-to-year variation of the response to radiation-induced stress shown by gill fatty acid metabolism in eels (anguilla anguilla) captured from the wild.1. in eels captured in roskilde fjord in 1972 and 1975, a specifically enhanced synthesis was found from 14c-acetate of 14c-labelled mono-unsaturated fatty acids (c16:1 and c18:1) relative to saturated fatty acids (c16:0 and c18:0) in sea water 4 days after irradiation (10 gy, 60co). 2. corresponding experiments in 1976 and 1982 showed rather the opposite: irradiation resulted in more 14c-labelled saturated fatty acids relative to unsaturated fatty acids, both in fresh and sea water. 3. the latt ...19911677881
in vitro study on the impact of fish sera on the survival and fine structure of the eel-pathogenic acanthocephalan paratenuisentis ambiguus.the effects of fish sera on the growth and fine structure of infective larvae of the eel-pathogenic acanthocephalan paratenuisentis ambiguus (eoacanthocephala: tenuisentidae) were studied under in vitro conditions using sera from the final host anguilla anguilla and from two accidental fish hosts as well as fetal calf serum. as controls larvae were also kept in medium in the absence of serum and in experimentally infected eels. sera from the accidental fish hosts carp and rainbow trout exerted t ...19911805215
comparative acute toxicities of selected pesticides to anguilla anguilla.the acute toxicities (24, 48, 72 and 96 hr) of eight pesticides to anguilla anguilla were determined. the organochlorine pesticide, endosulfan was the most toxic, with lc50 values in the range of 0.042 to 0.041 mg/l. endosulfan was followed in order of decreasing toxicity by diazinon, fenitrothion, chlorpyrifos, lindane, methidathion, trichlorfon and methylparathion. when fishes were exposed to the pesticides tested they exhibited signs of restlessness, erratic swimming, convulsions and difficul ...19911723417
histochemical, connectional and cytoarchitectonic evidence for a secondary reduction of the pretectum in the european eel, anguilla anguilla: a case of parallel evolution.there are at least three different patterns of pretectal organization in teleost fishes: a simple pattern observed in cyprinids, an elaborate pattern present in percomorphs, and an intermediately complex pattern seen in many other teleost groups. the taxonomic distribution of the pretectal patterns indicates that the simple and the elaborate patterns are both evolutionarily derived (apomorphic) from the primitive (plesiomorphic) intermediately complex one. in anguillids, the pretectal pattern ob ...19911764633
comparative study of acetylcholine synthesis in organs of freshwater teleosts.the activity of acetylcholine (ach) synthesizing enzyme, choline acetyltransferase (chat), and the content of ach were determined in the brain, heart, red trunk muscle and midgut of 12 freshwater teleost species belonging to eight families: carp (cyprinus carpio), tench (tinca tinca), silver carp (hypophthalmichtys molitrix), bighead (hypophthalmichtys nobilis), wels (silurus glanis), cat fish (ictalurus nebulosus), eel (anguilla anguilla), rainbow trout (oncorhynchus mykiss), pike (esox lucius) ...199124214667
the primary structure of glucagon-like peptide but not insulin has been conserved between the american eel, anguilla rostrata and the european eel, anguilla anguilla.insulin was isolated from the pancreas of the american eel, anguilla rostrata, and its primary structure was established as (formula: see text). eel insulin contains unusual substitutions at b-21, b-22, and b-26 in the putative receptor-binding region of the molecule compared with other mammalian and fish insulins. the a-chain of insulin from the european eel contains an asparagine rather than a serine residue at position a-12. similarly, amino acid composition data indicate the b-chain of insul ...19911874385
effects of vasoactive intestinal polypeptide, substance p, 5-hydroxytryptamine, met-enkephalin and neurotensin on the swimbladder smooth muscle of two teleost species,gadus morhua andanguilla anguilla.the effects of vasoactive intestinal polypeptide (vip), substance p (sp), 5-hydroxytryptamine (5-ht), neurotensin (nt) and met-enkephalin (menk) on the smooth muscles of the teleost swimbladder were studied in two teleost species, the atlantic cod (gadus morhua) and the eel (anguilla anguilla). the study was made on isolated strip preparations of the muscularis mucosae, using putative transmitters corresponding to the immunoreactive materials that have previously been localized by immunohistoche ...199124214612
effects of 17 beta-estradiol and carp gonadotropin on vitellogenesis in normal and hypophysectomized european silver female eel (anguilla anguilla l.) employing a homologous radioimmunoassay for vitellogenin.the european silver eels are at the early stages of vitellogenesis before the marine reproductive migration. vitellogenesis was induced by 17 beta-estradiol (e2) alone and by a purified carp gonadotropin (cgth). we studied and compared their effects on plasma vitellogenin (vg) levels and ovarian yolk contents in female normal (n) and hypophysectomized (h) eels for both treatments. to this purpose an homologous radioimmunoassay (ria) was established. eel vg was purified to homogeneity on 0.1% sds ...19911783271
regulation of secretion of the teleost fish hormone stanniocalcin: effects of extracellular calcium.the release in vivo and in vitro of stanniocalcin (stc) from the corpuscles of stannius (cs) of the rainbow trout and the european eel was studied. intraperitoneal injection of cacl2 (2.45 mmol.kg-1 fish) leads to an elevation of both ionic and total calcium in the plasma and results in the release of stc from the cs into the blood. release of stc in vitro is not affected at "physiological" (1.0-1.5 mm) or lower ca2+ levels in the incubation medium. high levels of ca2+ (2.5 mm and higher), howev ...19911778405
induction of biotransformation in the liver of eel (anguilla anguilla l.) by sublethal exposure to dinitro-o-cresol: an ultrastructural and biochemical study.structural and functional alterations in hepatocytes of the european eel, anguilla anguilla, following a 4-week-exposure to 5, 50, and 250 micrograms/liter dinitro-o-cresol (dnoc) were investigated by means of electron microscopy and biochemistry and compared to liver pathology in eels exposed to the chemical spill into the rhine river at basle in november 1986. whereas phenological parameters (growth, condition factor) are unaffected, ultrastructural and biochemical alterations are detectable a ...19912065626
changes in selected biochemical parameters in the brain of the fish, anguilla anguilla (l.), exposed to lindane. 19911722731
regulation of the type-ii gonadotrophin alpha and beta subunit mrnas by oestradiol and testosterone in the european eel.the gonadotrophic function of the european eel (anguilla anguilla l.) at the silver stage is very weak: gonadotrophin-releasing hormone (gnrh) secretion is deficient and, moreover, dopamine inhibition overrides gnrh action. at the silver stage, oestradiol stimulates the biosynthesis of the type-ii gonadotrophin (gth-ii). to study the molecular mechanism of this activation further, we examined the effect of testosterone and oestradiol administration on pituitary levels of mrna encoding gth-ii alp ...19911892544
age-related decrease in the density of benzodiazepine recognition sites in the brain of the fresh water eel (anguilla anguilla).the binding parameters of benzodiazepine receptors labeled with [3h]flunitrazepam were investigated in the brain of male adult eels at the trophic phase (4 years old) and of male silver eels (6 year old ocean migrants). the density of [3h]flunitrazepam binding sites was significantly decreased (-26%) in the brain of silver eels compared with younger counterparts. in contrast, the apparent dissociation constant (kd) was not significantly different in the two experimental groups. these results are ...19911667812
prostaglandins in the kidney, urinary bladder and gills of the rainbow trout and european eel adapted to fresh water and seawater.prostaglandins in kidney, gills, and urinary bladder of freshwater-adapted and seawater-adapted rainbow trout, oncorhynchus mykiss (= salmo gairdneri), and european eel, anguilla anguilla, were determined by solid-phase extraction of tissue homogenates and high-pressure liquid chromatography. prostaglandins e2, e1, f1 alpha, f2 alpha, and d2 and the more stable metabolite of prostacyclin, 6-keto f1 alpha, occurred in these osmoregulatory tissues. in gill filaments and kidneys of both eel and tro ...19911783277
lectins from triticum vulgaris and limax flavus are universal antagonists of botulinum neurotoxin and tetanus toxin.lectins from anguilla anguilla, artocarpus integrifolia, canavalia ensiformis, datora stramonium, glycine max, limax flavus, ricinus communis and triticum vulgaris were tested for their abilities to antagonize the binding of botulinum neurotoxin and tetanus toxin to rat brain membranes and to antagonize the ability of these toxins to block neuromuscular transmission in mouse phrenic nerve-hemidiaphragm preparations. lectins from limax flavus and triticum vulgaris, both of which have affinity for ...19911653841
occurrence of acanthocephalus clavula (acanthocephala) in echinogammarus pungens (amphipoda) from the river adige.cystacanths of acanthocephalus clavula dujardin 1845 (acanthocephala) were found in the hemocoels of naturally infected echinogrammarus pungens m. edwards 1840 (amphipoda). crustaceans were collected in porto frossone, an estuarine locality of the river adige. only 3 out of 562 e. pungens specimens were infected by a. clavula cystacanths; the larvae were enclosed in a thin acellular envelope. infected amphipods were all females, and their total length was greater than that of uninfected females. ...19911844501
in vivo and in vitro toxicity of fractionated fish lipids, with particular regard to their content of chlorinated organic compounds.six different lipid matrices (the intact lipid (il), four lipid fractions with different polarity, and the free fatty acids (ffas) obtained by hydrolysis of the triacylglycerol (tag) containing fraction) were obtained from salmon (salmo salar) and eel (anguilla anguilla), each collected at a contaminated and a comparatively uncontaminated catch site along the coast of scandinavia. the lipid matrices were studied in toxicological test systems representing various biological functions of different ...19911766922
regeneration of lateral line and inner ear vestibular cells.labelling experiments with [3h]thymidine demonstrate a continuous production of cells in the mechanoreceptive lateral line organs of the eel (anguilla anguilla) and butterfly fish (pantodon buchholzi) as well as in the electroreceptive ampullary organ of the transparent catfish (kryptopterus bicirrhus). shortly after [3h]thymidine injection many cells are labelled in the middle and basal parts of the sensory organ and after a few days' survival sensory cells are also labelled. the vestibular sen ...19911752161
effects of lindane on fish carbohydrate metabolism.exposure of the european eel (anguilla anguilla) to a high sublethal concentration of 0.335 ppm (0.50 of the 96-hr lc50) of lindane for 6, 12, 24, 48, 72, and 96 hr affected carbohydrate metabolism. muscle glycogen levels decreased significantly (p less than 0.05) at 6, 12, 24, 48, 72, and 96 hr; liver glycogen content did not decline at any time. muscle glucose levels in fish were elevated at 6, 12, 24, 48, 72, and 96 hr but in liver, the levels increased only at 96 hr. mean values of muscle an ...19911717227
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