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histochemical analysis of glycoconjugate secretion in the alimentary canal of anguilla anguilla l.conventional histochemical methods as well as lectin-binding techniques were used to study glycoconjugates that are present in the alimentary canal of the european eel (anguilla anguilla). specimens from pharynx, oesophagus, stomach and intestine were collected from adult ("silver eel" stage) females. alcian blue ph 2.5/pas and high iron diamine/alcian blue ph 2.5 reactions were performed to stain neutral and acidic glycoconjugates. in addition, lectin histochemistry was applied to identify acid ...200515707657
hypotonicity induced k+ and anion conductive pathways activation in eel intestinal epithelium.control of cell volume is a fundamental and highly conserved physiological mechanism, essential for survival under varying environmental and metabolic conditions. epithelia (such as intestine, renal tubule, gallbladder and gills) are tissues physiologically exposed to osmotic stress. therefore, the activation of 'emergency' systems of rapid cell volume regulation is fundamental in their physiology. the aim of the present work was to study the physiological response to hypotonic stress in a salt- ...200515695766
cell volume regulation following hypotonic stress in the intestine of the eel, anguilla anguilla, is ca2+-dependent.the involvement of ca2+ in the regulatory volume decrease (rvd) mechanism was studied in both isolated enterocytes and intestine of the eel, anguilla anguilla. videometric methods and electrophysiological techniques were respectively employed. the isolated enterocytes rapidly swelled following a change from isotonic (315 mosm/kg) to hypotonic (180 mosm/kg) saline solutions. afterwards, they tended to recover their original size. this homeostatic response was inhibited both in the absence of extr ...200515694583
environmental stress and life-stage dependence on the detection of heterozygosity-fitness correlations in the european eel, anguilla anguilla.heterozygosity-fitness correlations (hfcs) have been reported in populations of many species, although hfcs can clearly vary across species, conspecific populations, temporal samples, and sexes. we studied (i) the temporal stability of the association between genetic variation and growth rate (length and mass increase) and (ii) the influence of genetic variability on survival in the european eel (anguilla anguilla l). hfcs were assessed using genotypes from 10 allozyme and 6 microsatellite marke ...200617426758
stocking density at early developmental stages affects growth and sex ratio in the european eel (anguilla anguilla).to investigate the effect of stocking density on growth and sex ratio in european eel, four constant density conditions were tested during the transition from the glass to the elver stage for 90 days (period 1). the test conditions combined the weight of fish per unit surface or volume (surface density or volume density) resulting in four experimental groups: low surface density (0.5 kg/m(2)) and low volume density (5 kg/m(3)) (group s(0.5)v(5)); low surface density (0.5 kg/m(2)) and high volume ...200617179387
beta3-adrenoceptor in the eel (anguilla anguilla) heart: negative inotropy and no-cgmp-dependent mechanism.neuroendocrine regulation of cardiac function involves a population of three types of beta-adrenoceptors (ars). in various mammalian species, beta1- and beta2-ar stimulation produces an increase in contractility; whereas beta3-ar activation mediates negative inotropic effects. at the moment, nothing is known about the physiological role of beta3-ar in fish. using an isolated working heart preparation, we show that a beta3-ar selective agonist brl(37344) (0.1-100 nmol l(-1)) elicits a dose-depend ...200617142685
the involvement of cytochrome p450 system in the fate of 2,4,6-trinitrotoluene (tnt) in european eel [anguilla anguilla (linnaeus, 1758)].tnt (2,4,6-trinitrotoluene) was the most common nitro aromatic explosive available in world war ii ammunitions. the presence of ordnance dumped at sea might represent a great concern for marine species living close to dumping sites and the toxicological properties of the chemicals released into the marine environments need to be evaluated. the aim of the present study is to investigate the involvement of cyp (cytochrome p450) system in the metabolism of tnt in marine organisms by using the europ ...200617073791
galanin and its binding sites in the brain of eels subjected to different osmolar conditions.a galanin (gal)-like peptidergic system was investigated in the brain of anguilla anguilla subjected to hyperosmolar and hypoosmolar conditions, by using antisera raised against porcine 1-29 gal. a group of immunoreactive perikaria was identified in the periventricular hypothalamus, in the ventral thalamus, in the pretectal areas and in the optic tectum. immunoreactive perikaria were present in the nucleus lateralis of the torus semicircularis in seawater (sw) adapted eels, and were absent in th ...200616917814
pcb levels in european eel (anguilla anguilla) from two coastal lagoons of the mediterranean.eels are exposed to pollutants due to their unusual life cycle and are vulnerable to contamination associated with sediment due to their diet, feeding habits and territoriality. since the 1980s, a decline in american and european eel populations has been recorded. the causes of this decline still are unknown but pollution from domestic and industrial effluents is known to be involved. since little data is available on pcb contamination in eels from italian waters, pcbs were measured in muscle of ...200616917727
mutation analysis of ras gene in the liver of european eel (anguilla anguilla l.) exposed to benzo[a]pyrene.ras is regarded as one of the most important genes involved in carcinogenesis. such genes have been characterised in several fish species and the presence of ras mutations have already been described in fish populations from hydrocarbon contaminated areas and following experimental exposure to specific contaminants. the aims of this study were to evaluate the dna integrity by comet assay, to isolate the normal ras gene of anguilla anguilla and analyse for the presence of ras gene mutations or ch ...200616828491
polycyclic aromatic hydrocarbon metabolites and 7-ethoxyresorufin o-deethylase activity in caged european eels.this study investigated the contribution of two biomarkers, bile polycyclic aromatic hydrocarbon (pah) metabolites and 7-ethoxyresorufin o-deethylase (erod), activity in the assessment of pah contaminated sites. european eels (anguilla anguilla) were caged in a freshwater stream upstream and downstream from local industrial effluent outlets. bile pah metabolites were recorded as fluorescent aromatic compounds by synchronous fluorescence spectroscopy and as a marker for total pah metabolism: 1-hy ...200616783620
evidence for isolation by time in the european eel (anguilla anguilla l.).life history traits of highly vagile marine species, such as adult reproductive success and larval dispersal, are strongly determined by oceanographic and climatic forces. nevertheless, marine organisms may show restricted dispersal in time and space. patterns of isolation by distance (ibd) have been repeatedly observed in marine species. if spawning time is a function of geographical location, temporal and spatial isolation, can easily be confounded or misinterpreted. in this study, we aimed at ...200616780427
oxidative stress, liver biotransformation and genotoxic effects induced by copper in anguilla anguilla l.--the influence of pre-exposure to beta-naphthoflavone.fish are exposed in the aquatic ecosystems to different classes of pollutants. polycyclic aromatic hydrocarbons (pahs) and heavy metals represent two important classes of aquatic contaminants. thus, one lot of european eels (anguilla anguilla l.) was pre-exposed during 24 h to 2.7 microm beta-naphthoflavone (bnf; a pah-like compound), and subsequently exposed during 24 h to 0, 1 and 2.5 microm copper (cu). additionally, another lot not pre-exposed to bnf was exposed to the same cu concentrations ...200616735051
morphometry characterisation of european eel spermatozoa with computer-assisted spermatozoa analysis and scanning electron microscopy.the aim of the present study was to characterise european eel spermatozoa morphometrically, as a basis for future studies on the morphological effects of methods for sperm cryopreservation and sperm quality. this characterisation was carried out measuring several spermatozoa morphology parameters (head length, width, area and perimeter) by scanning electron microscopy (sem), in comparison with measurements developed in european eel spermatozoa with computer-assisted morphology analysis (asma). s ...200616185761
analysis of vitellogenin gene induction as a valuable biomarker of estrogenic exposure in various mediterranean fish species.several pollutants have the potential to disrupt the endocrine system in aquatic organisms, and synthesis of vitellogenin (vtg) in male fish is a well-recognized effect of estrogenic xenobiotics. in this respect both the presence of the protein in plasma and the analysis of vtg gene induction may represent valuable biomarkers. the present article describes primers specifically designed for a rt-pcr assay of vtg mrna in various mediterranean fish species. all the species analyzed have great poten ...200616168407
natural hybrids in atlantic eels (anguilla anguilla, a. rostrata): evidence for successful reproduction and fluctuating abundance in space and time.the outcome of natural hybridization is highly variable and depends on the nonexclusive effects of both pre- and post-mating reproductive barriers. the objective of this study was to address three specific questions regarding the dynamics of hybridization between the american and european eels (anguilla rostrata and anguilla anguilla). using 373 aflp loci, 1127 eels were genotyped, representing different life stages from both continents, as well as multiple icelandic locations. we first evaluate ...200616689906
androgen-dependent stimulation of brain dopaminergic systems in the female european eel (anguilla anguilla).dopamine (da), a neurotransmitter present in all vertebrates, is involved in processes such as motor function, learning and behavior, sensory activities, and neuroendocrine control of pituitary hormone release. in the female eel, we analyzed how gonadal steroids regulate brain expression of tyrosine hydroxylase (th), the rate-limiting enzyme in the biosynthesis of da. th mrna levels were assayed by quantitative real-time rt-pcr. th-positive nuclei were also localized by in situ hybridization (is ...200616543374
are dioxin-like contaminants responsible for the eel (anguilla anguilla) drama?eel populations worldwide are dangerously close to collapsing. our study is the first to show that current levels of dioxin-like contaminants are strong candidates because of their devastating effects on development and survival of eel embryos. female and male silver eels were artificially stimulated to maturation and reproduction by treatment with carp pituitary extracts and hcg, respectively. during maturation of female european silver eels, about 60 g fat per kg eel is incorporated in the ooc ...200616508793
oceanic biology: spawning of eels near a seamount.discoveries of the larvae of the european and american eels, anguilla anguilla and a. rostrata, in the sargasso sea and of the japanese eel, a. japonica, in the philippine sea indicate that these freshwater eels migrate thousands of kilometres into the open ocean to spawn. here we pinpoint a spawning location for japanese eels after genetically identifying newly hatched larvae that we collected from the site. the restricted size of this spawning area ensures that the eel larvae enter a particula ...200616495988
effect of the flavonol quercetin on ion transport in the isolated intestine of the eel, anguilla anguilla.flavonoids are phenolic compounds used in fish diet formulations for the control of sex differentiation. consequently it is of interest to know their effects on fish gastrointestinal mucosa that is the first target of these substances after food intake. we studied the effects of the flavonol quercetin on the transepithelial electrical parameters of the isolated intestine of the eel, anguilla anguilla, by employing ussing chamber technique. we showed that luminal quercetin (10(-4) m) reduced the ...200616466967
the eel immune system: present knowledge and the need for research.the european eel, anguilla anguilla, is one of the most important warm water fish species cultured in southern europe and the mediterranean as well as in northern countries including germany, the netherlands and denmark. the japanese eel, a. japonica, is an important cultured fish in several asiatic countries including japan, china and taiwan. during recent decades, research has been performed to elucidate the immune response of these species against different pathogens (viruses, bacteria or par ...200616436117
effects of high-pressure acclimatization on silver eel (anguilla anguilla, l.) slow muscle contraction.as the spawning migration of the eel is supposed to correspond to a long swimming activity at depth, patterns of slow red muscle contraction have been investigated in european silver eel (anguilla anguilla l.) exposed for 3 weeks to 10.1 mpa hydrostatic pressure. the results show that pressure-acclimated eels (male and female) show a three-fold decrease in maximum isometric stress of twitch and tetanic contractions while time to peak force, time from peak force to 90% relaxation and ratio of twi ...200616413804
possible role of calcitonin gene-related peptide in osmoregulation via the endocrine control of the gill in a teleost, the eel, anguilla anguilla.osmoregulation is a major challenge in aquatic animals involving a complex endocrine control. we investigated the potential role of calcitonin gene-related peptide (cgrp, a neuromediator in mammals) in the endocrine control of the gill in a teleost, the eel. transfer from freshwater to seawater induced an hyperosmolality and a concomitant large increase in plasma cgrp levels. specific cgrp binding sites were characterized in the gill and their number was up-regulated after seawater transfer. thi ...200616253389
characterization of a binary tandem domain f-type lectin from striped bass (morone saxatilis).among other functions, lectins play an important role in the innate immune response of vertebrates and invertebrates by recognizing exposed glycans on the surface of potential pathogens. despite the typically weak interaction of lectin domains with their carbohydrate ligands, they usually achieve high avidity through oligomeric structures or by the presence of tandem carbohydrate-binding domains along the polypeptide. the recently described structure of the fucose-binding european eel agglutinin ...200616251191
responses of european eel (anguilla anguilla l.) circulating phagocytes to an in situ closed pulp mill effluent exposure and its association with organ-specific peroxidative damage.the effect of bleached kraft pulp mill effluent (bkpme) persistent compounds on phagocyte activities and its organ-specific influence in gill, kidney and liver was studied in european eel (anguilla anguilla l.). eels were caged and plunged at 3 different sites-50 m (site 1), 100 m (site 2) and 2,000 m (site 3) away from the end of the closed bkpme discharging channel for 8 and 48 h. gill, head kidney and peritoneum phagocytes oxidative burst activity (oba) was measured by the nitroblue tetrazoli ...200616229874
epidermal proteinases in the european eel.a fluorescent-sensitive assay demonstrated the exhaustive detection of proteinase activities in the dorsal skin of the european eel. two distinct skin extracts were prepared from skin mucus and epidermal cell layers with no mutual contamination, so that the latter extract contained significant susceptibility of all tested substrates. optimum hydrolysis ph's for susceptible substrates were found in acidic and neutral ranges, and optimum hydrolysis temperatures for the same substrates fell mainly ...20069279923
effect of deterlon (dbs) on the electrocardiogram of eel anguilla anguilla (l).effect of deterlon (dbs) on the electrocardiogram of eel anguilla anguilla (l). acta physiol. pol. 1977, 28 (2): 179--186. the investigations of the effect of a detergent deterlon-dbs present in industrial and community sewage on ecg changes in the eel anguilla anguilla (l) showed that it causes disturbances in the impulse-generating centres and in the mechanism of impulse conduction in the heart. it was found that the threshold concentration of deterlon-dbs in aqueous medium is 3 mg sa/1 for th ...2006868536
heavy metal profile in five fish species included in human diet, domiciled in the end flow of river neretva (croatia).concentration of three heavy metals (mercury (hg), lead, (pb), and cadmium, (cd)) and one metalloid (arsenic [as]), were determined by atomic absorption spectrophotometry in five tissues (muscles, liver, kidneys, gills, and gonads) of five fish species (carp-cyprinus carpio, tench-tinca tinca, sval-leuciscus svallizi, gray mullet-mugil cephalus, and eel-anguilla anguilla) taken out from the end fiow (last 20 km) of the river neretva, south croatia, in the wider region of town metković, during th ...200616453065
anguilla anguilla l. genotoxic responses after in situ exposure to freshwater wetland (pateira de fermentelos, portugal).pateira de fermentelos is a cértima river enlargement, close to its river mouth (by the agueda river), where the introduction of agricultural chemicals such as fertilisers and pesticides, domestic sewage, as well as heavy metals from electroplating industries, results in increased water pollution. the present research work concerns a 48 h in situ exposure of caged eels (anguilla anguilla l.) at the pateira de fermentelos. five exposure sites were selected, i.e., site a, site b, site c, site d an ...200616483656
sexually mature european eels (anguilla anguilla l.) stimulate gonadal development of neighbouring males: possible involvement of chemical communication.this study was aimed to investigate whether sexual maturation of immature male eels could be stimulated indirectly by placing them in contact with either male (minj) or female (finj) eels in which sexual maturation had been stimulated directly by weekly injections of human chorionic gonadotropin (hcg) or salmon pituitary extract (spe), respectively. untreated males were placed either in the same tank or in a separate tank that was linked to the injected fish via a recirculation system. the hormo ...200616545383
effects of hcg as spermiation inducer on european eel semen quality.fish sperm quality has traditionally been estimated by subjective evaluation of motility and sperm concentration. alternative methods for evaluation of sperm quality have been developed in the last decade and enable estimation of spermatozoa head morphometry, membrane integrity and mitochondrial function. weekly injections of human chorionic gonadotropin (hcg) induced spermiation in farmed male european eels. the milt volume increased from the 5th to 12th weeks. sperm concentration significantly ...200616595151
cryopreservation of european eel (anguilla anguilla) spermatozoa: effect of dilution ratio, foetal bovine serum supplementation, and cryoprotectants.the main aim of the present study was to investigate the effect of sperm freezing medium dilution ratio (1:1, 1:2, and 1:5 v/v), two cryoprotectants: dimethyl sulphoxide (me(2)so) and methanol (meoh), and the addition of foetal bovine serum (fbs) on the cryopreservation of european eel sperm. the effect of these factors was evaluated comparing post-thawing viability with fluorescent staining (hoechst bisbenzimide 33258) and the spermatozoa head morphometry, determined with computer-assisted morp ...200616712830
biomarker responses in european eel (anguilla anguilla) exposed to persistent organic pollutants. a field study in the vaccarès lagoon (camargue, france).a screening of relevant biomarkers was carried out in order to evaluate metabolic and cellular damages in european eels exposed to a non-point source contamination by persistent organic pollutants (pop) such as polycyclic aromatic hydrocarbons (pah) and organochlorine compounds (oc) in a protected area, the nature reserve of camargue (france). investigations were focused on metabolic responses including detoxification mechanisms (biotransformation, antioxidant process), energy requirements and e ...200616714045
anguilla anguilla l. oxidative stress biomarkers: an in situ study of freshwater wetland ecosystem (pateira de fermentelos, portugal).pateira de fermentelos (pf) is a natural freshwater wetland in the central region of portugal. in the last decade, the introduction of agricultural chemicals, heavy metals, domestic wastes, as well as eutrophication and incorrect utility of resources resulted in an increased water pollution. the present research work was carried out to check the various oxidative stress biomarker responses in european eel (anguilla anguilla l.) gill, kidney and liver due to this complex water pollution. eels wer ...200616725179
influence of biological and ecological factors on the bioaccumulation of polybrominated diphenyl ethers in aquatic food webs from french estuaries.previous studies have shown the worldwide presence of six congeners of polybrominated diphenyl ethers (pbdes) in marine biota (bde-28, -47, -99, -100, -153 and -154). the objective of the present study was to document their presence, their level and their transfer in the food web of two major estuaries in france, the loire and the seine. pbdes were quantified in eight principal species from the loire, representing primary consumers (the bivalve scrobicularia plana), omnivores (the worm nereis di ...200616764911
the swimbladder nematode anguillicola crassus in the european eel anguilla anguilla and the japanese eel anguilla japonica: differences in susceptibility and immunity between a recently colonized host and the original host.the swimbladder nematode anguillicola crassus originates from asia where it is a parasite of the japanese eel anguilla japonica. after its introduction to europe about 25 years ago, the parasite spread rapidly within the indigenous populations of the european eel anguilla anguilla and subsequently the prevalence and mean intensity appeared to stabilize. under experimental and aquaculture conditions the naïve new host appears to be more susceptible to a. crassus compared to the original host. bot ...200616768856
oxidative stress and genotoxic effects in gill and kidney of anguilla anguilla l. exposed to chromium with or without pre-exposure to beta-naphthoflavone.fish in the aquatic environment can be subjected to a multipollution state and the occurrence of sequential exposures is an important aspect of eco-toxicological research. in this context, a preceding exposure can affect a toxic response to a subsequent exposure. therefore, the current study was based on sequential exposures, viz. to a pah-like compound (beta-naphthoflavone, bnf) followed by a heavy metal (chromium, cr), focusing on the assessment of oxidative stress responses and their role in ...200616784884
selective supercritical fluid extraction to identify aged sediment-bound pcbs available for uptake by eel.a naturally contaminated sediment was partially extracted with selective supercritical fluid extraction (sfe) to remove a fraction of supposedly bioavailable pcbs from the matrix. eels (anguilla anguilla) were cultured in systems with untreated and pre-extracted sediment, respectively, and it was shown that the sfe treatment selectively removed bioavailable pcbs from the sediment, since relative biota-to-sediment accumulation factors (bsafs) for the eight studied pcb congeners were much lower in ...200616188355
effects of infection with anguillicola crassus and simultaneous exposure with cd and 3,3',4,4',5-pentachlorobiphenyl (pcb 126) on the levels of cortisol and glucose in european eel ( anguilla anguilla ).to investigate whether the stress response of european eels infected with anguillicola crassus is influenced by environmental pollutants, experimentally infected eels were exposed to cd and/or to 3,3', 4,4', 5-pentachlorobiphenyl (pcb 126). serum cortisol and glucose concentrations of these eels were monitored over a period of 103 days and were compared with data from infected, unexposed eels as well as with data from uninfected eels. additionally, the levels of cortisol were correlated with con ...200616209723
availability of in vitro vitellogenin assay for screening of estrogenic and anti-estrogenic activities of environmental chemicals.vitellogenin (vtg) protein, vtg mrna, other egg yolk proteins, vitelline envelope proteins and their mrnas are produced in the liver of oviparous species by stimulation of endogenous estrogen and exogenous estrogenic chemicals. the vtg assay based on enzyme-linked immunosorbent assay (elisa) has been widely used for many fish species to screen estrogenic and anti-estrogenic activities of chemicals and sewage effluents using immature fish and/or male fish. in order to reduce the number of fish fo ...200616883298
parasite fauna of the eel, anguilla anguilla (linnaeus, 1758), from the polish part of the vistula lagoon.eel is one of the most valuable commercial fish species of the vistula lagoon. for this reason its infection by non-indigenous species of parasites is the subject of the interest of sciencists.200617120993
parasite fauna of the european eel (anguilla anguilla l, 1758) from the russian part of the vistula lagoon (baltic sea).the european eel is one of the major fishing objects of the russian fishery zone of the vistula lagoon (baltic sea). this is the reason that its parasite fauna was studied within 1998-2005.200617120994
presence of lactobacilli in the intestinal content of freshwater fish from a river and from a farm with a recirculation system.lactobacilli are gram-positive and catalase negative rods commonly found in lactic acid fermented foods and in the gastrointestinal tract of mammals and birds. few studies have described lactobacilli in freshwater fish. we analysed the presence of lactobacilli in the intestines of young and adult freshwater fish inhabiting a river environment and from fish reared in an aquaculture unit with a water recirculation system. various species of lactobacilli were present in relatively high number in th ...200616943040
blood group antigen recognition by a streptococcus pneumoniae virulence factor.the streptococcus pneumoniae fucose utilization operon includes a gene encoding a virulence factor that belongs to family 98 in the glycoside hydrolase classification. this protein contains a c-terminal triplet of fucose binding modules that have significant amino acid sequence identity with the anguilla anguilla fucolectin. functional studies of these fucose binding modules reveal binding to fucosylated oligosaccharides and suggest the importance of multivalent binding. the high resolution crys ...200616987809
spatio-temporal dynamics of the nematode anguillicola crassus in northeast tunisian lagoons.anguillicola crassus, parasite nematode of the european eel anguilla anguilla, was recorded for the first time in tunisia (1999) in the ichkeul lagoon. its distribution has since spread toward bizerte and ghar el melh lagoons. the monthly epidemiological survey reveals that a. crassus exists throughout the year in the ichkeul lagoon. in this lagoon, its prevalence is low in winter (12% in december), with a marked increases in the spring reaching a maximum in march (35%), before it starts to decr ...200617027639
genotyping of marine viral haemorrhagic septicaemia virus isolated from the flemish cap by nucleotide sequence analysis and restriction fragment length polymorphism patterns.a total of 14 viral haemorrhagic septicaemia virus (vhsv) isolates obtained from greenland halibut reinhardtius hippoglossoides caught at the flemish cap, a fishing ground in the north atlantic ocean near newfoundland, were characterised using restriction fragment length polymorphism (rflp) and nucleotide sequence analysis. rflp analysis was performed on a 1259 bp fragment of the glycoprotein (g) gene, and a 305 nucleotide region within the nucleoprotein (n) gene was used for sequence analysis. ...200617240749
occurrence of edwardsiella tarda in wild european eels anguilla anguilla from mediterranean spain.pure cultures of edwarsiella tarda were isolated from body ulcers and internal organs of wild european eels caught in a mediterranean freshwater coastal lagoon (albufera lake, valencia, spain) over a 1 yr period. overall, the e. tarda isolation rate from wild eels was 9%, but this increased to 22.8% in diseased individuals. all 22 e. tarda isolates belonged to the 'wild-type' biogroup of the species and were virulent for eels (lethal dose that kills 50% of exposed individuals [ld50 dose]: 10(4.8 ...200617240756
[evolutive cycle, in a single intermediate host, of bothriocephalus claviceps (goeze, 1782), cestode of anguilla anguilla l]. 200614220586
temperature acclimation and oxygen binding properties of blood of the european eel, anguilla anguilla.temperature acclimation of the european eel, anguilla anguilla, resulted in red cell gtp/hb molar ratios of 1.20, 1.77 and 0.80 at 2, 17 and 29 degrees c, respectively. a small increase in blood oxygen capacity was present in 29 degrees c acclimated eels. the co2 bohr effect and the shape of the oxygen binding curve (n-hill) were invariant with both temperature and gtp/hb. the significant differences in the gtp/hb ratio corresponded with a strong enhancement of the temperature effect on blood ox ...20062859959
[influence of the pituitary on the levels of corticosteroids of the plasma of the eel (anguilla anguilla l.)]. 200613760698
water and electrolyte flux in the european eel (anguilla anguilla l.). 200714337072
ultrastructure of the spermatozoon of bothriocephalus claviceps (cestoda, pseudophyllidea): a parasite of anguilla anguilla (fish, teleostei).the mature bothriocephalus claviceps spermatozoon is filiform and tapered at both extremities. it contains two axonemes of unequal lengths of the 9+"1" pattern of the trepaxonemata. the anterior extremity exhibits an apical cone of an electron-dense material about 6.6 microm long by 0.2 microm wide at its base and a crest-like body making an angle of about 40 degrees with the spermatozoon axis. it is spiralized around the whole extremity of the spermatozoon. the nucleus is a cord of electron-den ...200717235546
thyroid hormone-induced demineralisation of the vertebral skeleton of the eel, anguilla anguilla.the role of thyroid hormones (th) in bone remodelling is controversial. indeed, in humans, while they are necessary for normal growth and development, their overproduction can induce important mineral bone loss and osteoporosis. intense bone resorption is a natural phenomenon also observed in some teleosts, during reproductive migration and fasting. our work aimed at investigating the effects of chronic treatments with th (thyroxin, t4 or triiodothyronine, t3) on bone resorption in a migratory f ...200717280664
dioxins and dioxin-like pcbs in different fish from the river elbe and its tributaries, germany.in a long-term program polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans (pcdd/fs) as well as dioxin-like polychlorinated biphenyls (dl-pcbs) were analyzed in the muscle tissue of eels (anguilla anguilla), bream (abramis brama), european chub (leuciscus cephalus) and ide (leuciscus idus) from the river elbe and its tributaries mulde and saale. the variation of the pcdd/f and dl-pcb concentrations in all fish samples is very large, whereby the dl-pcbs predominate in comparison t ...200717382467
detection of hexabromocyclododecane and its metabolite pentabromocyclododecene in chicken egg and fish from the official food control.during routine gas chromatography with electron capture detection (gc/ecd) analysis of chicken eggs, we observed that the most prominent peak in some samples did not match the retention time of any of the food contaminants screened. subsequent gc coupled with mass spectrometry (gc/ms) studies clarified that the mass spectrum of the peak was very similar to hexabromocyclododecane (hbcd), which was also identified by gc/ms in the egg. the unknown compound was positively identified as pentabromocyc ...200717419640
the pathogenic helminth parasites of eels.although 63 and 55 species of helminths have been reported from each species of atlantic eel and from 29 to 19 for each species of pacific eel only the monogeneans pseudodactylogyrus bini and p. anguillae and the nematode anguillicola crassus, originally specific to species of pacific eels, can be considered serious pathogens. none of the three are normally pathogenic to their preferred natural eel host species in the wild. pseudodactylogyrus spp. only cause serious local gill damage when presen ...200717498176
biochemical characterization of a s-glutathionylated carbonic anhydrase isolated from gills of the antarctic icefish chionodraco hamatus.gill cytoplasmic carbonic anhydrase of the haemoglobinless antarctic icefish chionodraco hamatus (ice-ca) was directly sequenced and consists in 259 residues with an acetylated n-terminus. the molecular mass, deduced from the sequence, was 28.45 kda, while mass spectrometry analysis of the native protein gave higher values. treatment with dithiothreitol abolished this difference, indicating possible post-translational modifications. isoelectric focusing analysis of ice-ca suggested s-thiolation, ...200717510781
methylmercury bioconcentration in muscle tissue of the european eel (anguilla anguilla) from the adour estuary (bay of biscay, france). 200717543349
do eel parasites reflect the local crustacean community? a case study from the rhine river system.in 2003, the parasite fauna of 197 european eels anguilla anguilla, captured at three different locations (laufenburg, karlsruhe and beneeden leeuwen) in the river rhine, was analysed. the eels harboured a total of 18 species, among them the protozoa (myxidium giardi, myxobolus kotlani and trypanosoma granulosum), acanthocephalans (acanthocephalus anguillae, acanthocephalus lucii, echinorhynchus truttae, pomphorhynchus laevis), nematodes (paraquimperia tenerrima, pseudocapillaria tomentosa, cama ...200717578598
quality attributes of farmed eel (anguilla anguilla) stored under air, vacuum and modified atmosphere packaging at 0 degrees c.the shelf life of fresh eel in various packaging conditions of atmospheric air, vacuum and modified atmosphere packaging (map) (40% co(2), 30% n(2) and 30% o(2)) at 0 degrees c was investigated. all raw eel samples received acceptable sensory scores during the first 11+/-1 days of storage in atmospheric air, 11+/-1 days of storage in vacuum and finally 18+/-1 days of storage in map conditions. using the microbial quality indicators the shelf life of eel packed in air, vacuum and map was estimate ...200717613370
phylogenetic identification of aeromonas strains isolated from carcasses of pig as new members of the species aeromonas allosaccharophila.the first description of the species aeromonas allosaccharophila was only based on two strains (the type strain cect4199, and a duplicate cect4200) isolated from diseased elvers (anguilla anguilla) of an eel-farm located in valencia, spain, and one stool isolate (atcc35942) from a female with diarrhoea and food poisoning in south carolina, u.s.a. in the present study, 17 aeromonas isolates obtained from carcasses of pigs and from the equipment for the cleaning process, and one strain recently is ...200717080292
direct calorimetry of free-moving eels with manipulated thyroid status.in birds and mammals, the thyroid gland secretes the iodothyronine hormones of which tetraiodothyronine (t4) is less active than triiodothyronine (t3). the action of t3 and t4 is calorigenic and is involved in the control of metabolic rate. across all vertebrates, thyroid hormones also play a major role in differentiation, development and growth. although the fish thyroidal system has been researched extensively, its role in thermogenesis is unclear. in this study, we measured overall heat produ ...200717082966
the dynamics of the nematode anguillicola crassus, kuvahara 1974 in eel anguilla anguilla (l. 1758) in the sebou estuary (morocco).the european eel is a vulnerable fish by its complex life cycle, by the impact of pollution of the near total of freshwater aquatic environments, and by the gravity and the diversity of its parasites (nematodes, cestodes, trematodes, the copepoda, coccidia...). it is classified in the red book of threatened species. the anguillulose is the principal parasitic pathology of an eel either in the natural environment or in the aquaculture. the eels taken in the three zones of the sebou estuary of var ...200717096144
endocrine and metabolic responses of anguilla anguilla l. caged in a freshwater-wetland (pateira de fermentelos--portugal).the present short-term in situ study was carried out in a freshwater-wetland--pateira de fermentelos--considered an enlargement of cértima river, in the centre of portugal. this ecosystem is an important fishing and recreation place, receiving effluents from different origins namely, electroplating industrial effluents containing heavy metals, domestic wastes, as well as pesticides and fertilizers resulting from agriculture activities. the aim of the present research work was to monitor the effe ...200717150242
metal and metallothionein content in tissues from wild and farmed anguilla anguilla at commercial size.metallothionein and metal content (cd, zn, hg, cu, fe, pb and mn) were determined in various organs of commercially available eel (anguilla anguilla) of similar size obtained from a local farm and from the albufera lake in valencia (spain). farmed fish showed statistically significant higher cd concentrations in liver and kidney whereas wild individuals had higher levels of pb in blood and zn in kidney. significant positive correlations were found between metallothionein and cd in kidney of farm ...200717157913
parasite communities of eels anguilla anguilla in freshwater and marine habitats in iceland in comparison with other parasite communities of eels in europe.ninety-five eels from one marine and three freshwater localities in iceland were examined for parasites. twenty species were found, 12 from marine habitat, 12 from freshwater and 4 species were found in both habitats. these are: eimeria anguillae, chilodonella hexasticha, trichodina fultoni, t. jadranica, myxidium giardi, myxobolus kotlani, two zschokkella spp., derogenes varicus, deropristis inflata, diplostonmum sp., plagioporus angulatus, podocotyle atomon, anisakis simplex (larva), eustrongy ...200717886743
pathogenesis of herpesvirus anguillae (hva) in juvenile european eel anguilla anguilla after infection by bath immersion.a clinical infection in post-larval (glass) european eels anguilla anguilla was successfully induced after artificial bath immersion with herpesvirus anguillae (hva), isolated from diseased european eel. hva caused a clinical infection after 7 d post-inoculation (pi); virus was detected by polymerase chain reaction (pcr) from day 1 pi; virus isolation was positive from day 7 pi, and hva antigen was detected by immunohistochemistry in gills and stomach from day 4 pi. tissue changes were found by ...200718159668
characteristics of the peripheral blood of the anguillicola crassus (kuwahara, niimi et tagaki, 1974) and trypanosoma granulosum laveran et mesnil, 1902 infested eel (anguilla anguilla l.) ascending the river rega (north-western poland).the study was aimed at characterising the peripheral blood of eel (anguilla anguilla l.) ascending the river rega and infested with anguillicola crassus (kuwahara, niimi et itagaki, 1974) and trypanosoma granulosum laveran et mesnil, 1902. material and methods: haematological assays were performed on 58 eel individuals caught in august and september 2001 and in july 2002. the nematode anguillicola crassus was identified in the swim bladder lumen of 44 eel individuals. the mean infestation intens ...200718441878
electroretinographic evaluation of spectral sensitivity in yellow and silver eels (anguilla anguilla).although differences in visual pigments between developmental stages of the european eel are well known, the expected differences in spectral sensitivity have not been demonstrated at the electrophysiological level. in fact, one past electroretinographic study led to the conclusion that in eels there is no change in scotopic sensitivity, with increasing sexual maturity. in the present experiments, electroretinograms (ergs) were recorded from in situ eyecups of immobilized eels anguilla anguilla ...20079764534
crucial role of cytoskeleton reorganization in the negative inotropic effect of chromogranin a-derived peptides in eel and frog hearts.vasostatins (vss), i.e. the main biologically active peptides generated by the proteolytic processing of chromogranin a (cga) n-terminus, exert negative inotropism in vertebrate hearts. here, using isolated working eel (anguilla anguilla) and frog (rana esculenta) heart preparations, we have studied the role of the cytoskeleton in the vss-mediated inotropic response. in both eel and frog hearts, vss-mediated-negative inotropy was abolished by treatment with inhibitors of cytoskeleton reorganizat ...200717056132
a preliminary observation on the pond culture of european eel, anguilla anguilla (linnaeus, 1758) in egypt: recommendations for future studies.an experiment was conducted to assess the potential of the european eel, anguilla anguilla for earthen pond aquaculture without supplementary feeding at lake manzala, egypt. juvenile a. anguilla of mean length 11.7 cm and 2.4 g weight were stocked in earthen ponds measuring 3 feddans (about 12,600 m2) and 1 m deep. stocking was done in may 2003 at a rate of 5000 fish feddan(-1) in a polyculture system including tilapia and mullets and fed mainly on natural occurring prey (natural spawned tilapia ...200719070049
effect of sperm cryopreservation on the european eel sperm viability and spermatozoa morphology.the main objective of the present work was to study the effect of cryopreservation of european eel sperm both on the sperm viability and the spermatozoa head morphology. spermatozoa morphology was evaluated with computer-assisted morphology analysis after collection in fresh samples, after adding the freezing medium containing dimethyl sulfoxide as cryoprotectant and, finally, after the cryopreservation process and thawing. cell viability was assessed, in both fresh and thawed samples, by hoechs ...200717348973
salinity adaptation and gene profiling analysis in the european eel (anguilla anguilla) using microarray technology.the life cycle of the european eel (anguilla anguilla) includes two long migratory periods, when the newly hatched leptocephali larvae drift on ocean currents from the sargasso sea to the shores of western europe and then again up to 30 years later when adult eels swim back to their place of birth for reproductive purposes. prior to the migration from fresh water (fw) to sea water (sw) adult yellow eels undergo various anatomical and physiological adaptations (silvering) which promote sexual dev ...200717324422
the role of volume-sensitive ion transport systems in regulation of epithelial transport.this review focuses on using the knowledge on volume-sensitive transport systems in ehrlich ascites tumour cells and nih-3t3 cells to elucidate osmotic regulation of salt transport in epithelia. using the intestine of the european eel (anguilla anguilla) (an absorptive epithelium of the type described in the renal cortex thick ascending limb (ctal)) we have focused on the role of swelling-activated k+- and anion-conductive pathways in response to hypotonicity, and on the role of the apical (lumi ...200717289411
impairment of lipid storage by cadmium in the european eel (anguilla anguilla).because european silver eels (anguilla anguilla) fast during their reproductive migration to the sargasso sea, the successful completion of their unusual life cycle depends on quantity of lipids stored beforehand. these lipids are mainly accumulated during the growth phase stage of the animals, called yellow eel, as triglycerides in muscle. they are then catabolized to provide sufficient energy to enable migration, gonad maturation and spawning. in the laboratory, we investigated the possible im ...200717276523
probabilistic approach to polychlorinated biphenyl (pcb) exposure through eel consumption in recreational fishermen vs. the general population.concentrations of the sum of the seven indicator pcbs (sigma7 ipcbs) measured in non-commercial european eel (anguilla anguilla l.) in flanders are high: in 80% of all sampled localities, the belgian pcb standard for fish was exceeded. the objective of this study was to assess the intake of the sigma7 ipcbs through consumption of eel by recreational fishermen and to compare it to the intake of a background population. the median estimated intake for recreational fishermen varied between 18.4 and ...200717852389
transcriptomic approach to the study of osmoregulation in the european eel anguilla anguilla.in euryhaline teleosts, osmoregulation is a fundamental and dynamic process that is essential for the maintenance of ion and water balance, especially when fish migrate between fresh water (fw) and sea water (sw) environments. the european eel has proved to be an excellent model species to study the molecular and physiological adaptations associated with this osmoregulatory plasticity. the life cycle of the european eel includes two migratory periods, the second being the migration of fw eels ba ...200717666525
nitric oxide modulates ionic transport in the isolated intestine of the eel, anguilla anguilla.we investigated the role of no (nitric oxide) in the isolated intestine of the sea water adapted eel, by testing the effect of various donors on i(sc) (short-circuit current), due to net cl(-) absorption in the control conditions. we found that the endogenous no-synthase substrate l-arginine as well as two different no donors, snp (sodium nitroprusside) and sin-1 (3-morpholinosydnonimine), produced a slow and gradual decrease of i(sc). the effect of snp was reduced by the pretreatment with odq ( ...200717604669
energy and migratory behavior in glass eels (anguilla anguilla).the influence of energy content of glass eels (anguilla anguilla) on their migratory behavior was investigated in november 2004 and february 2005 under laboratory conditions. fish were sorted according to their response to a decrease in light intensity: glass eels becoming active at dusk and using water flow to move with the current were considered as "active", whereas those remaining in the substratum throughout the catching period (24 h) were categorized as "inactive". "active" glass eels exhi ...200717561177
offshore windmills and the effects of electromagnetic fields on fish.with the large scale developments of offshore windpower the number of underwater electric cables is increasing with various technologies applied. a wind farm is associated with different types of cables used for intraturbine, array-to-transformer, and transformer-to-shore transmissions. as the electric currents in submarine cables induce electromagnetic fields there is a concern of how they may influence fishes. studies have shown that there are fish species that are magneto-sensitive using geom ...200718240676
common pattern of gene expression in response to hypoxia or cadmium in the gills of the european glass eel (anguilla anguilla).european eel (anguilla anguilla) populations are in decline. glass eel recruitment has fallen 10-fold since the early 1980s. estuaries play a fundamental role in the life history of eels because glass eels must pass through them to reach freshwater ecosystems. unfortunately, because of their geographical position at the upstream basin slopes, estuaries accumulate metals like cadmium and are important sites of hypoxia events. in this context, we studied the effect of the oxygen level on the venti ...200717533871
spermiogenesis and sperm ultrastructure of deropristis inflata molin, 1859 (digenea, deropristidae), a parasite of anguilla anguilla.to our knowledge, this paper describes the first ultrastructural results on spermiogenesis and the spermatozoon of a digenea belonging to the family deropristidae, deropristis inflata. spermiogenesis follows the usual pattern found in the digeneans, but a single noteworthy characteristic concerns the centriole, which presents a "prominent pear-shaped electron-dense region." spermiogenesis in d. inflata begins with the formation of a differentiation zone. the two centrioles give rise to flagella. ...200717484073
does a 5500-km swim trial stimulate early sexual maturation in the european eel (anguilla anguilla l.)?the catadromous european eel (anguilla anguilla l.) undertakes a 6000-km spawning migration from its freshwater habitats to the sargasso sea. in large blazka swim tunnels of 127 l, the physiological effect of such a prolonged swimming performance on sexual maturation in adult female eels was investigated. two groups of eels were placed in swim tunnels for 173 days, one group was able to swim at 0.5 body lengths/second (swim group) covering a distance of c. 5500-km over the experimental period, a ...200717451986
relationships between locomotor behavior, morphometric characters and thyroid hormone levels give evidence of stage-dependent mechanisms in european eel upstream migration.in order to decipher movements during freshwater eel colonization, we experimentally characterized individual locomotor behavior of two eel life history stages: elvers and yellow eels. a ramp located at the flume tank upstream side required a specific locomotor behavior to be ascended. placing individually tagged eels in the middle of the tank three times successively tested behavioral consistency. eels climbing the ramp on each trial were classified as "upstream climbers" whereas eels settling ...200817950736
differential expression of absorptive cation-chloride-cotransporters in the intestinal and renal tissues of the european eel (anguilla anguilla).duplicate pairs of isoforms of each of the nkcc2 and the ncc absorptive cation-chloride-cotransporters have been isolated from the european eel. as with mammalian nkcc2, nkcc2alpha isoform mrna expression was restricted to renal tissues, whereas nkcc2beta isoform expression was present in intestine and urinary bladder. similar to mammalian ncc, nccalpha mrna expression was also found in the kidney, whereas, expression of nccbeta mrna was found at low levels in a number of tissues but particularl ...200817950642
to what extent are hepatic concentrations of heavy metals in anguilla anguilla at a site in a contaminated estuary related to body size and age and reflected in the metallothionein concentrations?we explored how hepatic [metal]s in anguilla anguilla at a contaminated estuarine site are influenced by body size, age and season, and the extent that [cu], [cd] and [zn]s are reflected in [metallothionein (mt)]s. although each [metal] and [mt] increased significantly with length, weight and age, those biotic variables explained <10% of the variation in each [metal] and only 11-16% for [mt]. seasonal changes in [cu] and [cd] were paralleled by [mt]. the variation in [mt] explained by cu (42%) w ...200817548139
the suitability of cytochrome-p4501a1 as a biomarker for pcb contamination in european eel (anguilla anguilla).european eel (anguilla anguilla) elvers were intraperitoneally injected with different doses of 3,3',4,4'-tetrachlorobiyphenyl (pcb77) to examine and characterize the inductive effect of coplanar pcbs on cyp1a1 gene expression in liver and gills by using a semi-quantitative rt-pcr analysis. the influence of pcb77 injection on transcription activity of the housekeeping gene glyceraldehyde-3-phosphate-dehydrogenase (gapdh) was tested to determine its suitability as a reference gene for further qua ...200818657580
how cadmium could compromise the completion of the european eel's reproductive migration.the european eel (anguilla anguilla l.) is severely threatened with extinction. surprisingly, even though their unusual life cycle makes them particularly vulnerable to pollution, the possible contribution of contamination remains especially poorly known. here we have investigated the possible effect of cadmium (cd), a widespread nonessential metal, on eel reproductive capacities. both control and cd precontaminated female silver eels were experimentally matured and forced to swim in metal-free ...200818605594
cost of transport and optimal swimming speed in farmed and wild european silver eels (anguilla anguilla).a swimming speed of 0.4 meters per second (m s(-1)) is the minimal speed for european female silver eels to reach the spawning sites in the sargasso sea in time. as silver eels cease feeding when they start their oceanic migration, the cost of transport (cot) should be minimised and the swimming speed optimised to attain the highest energetic efficiency. in this study, we have investigated the optimal swimming speed (u(opt)) of silver eels since u(opt) may be higher than the minimal swimming spe ...200818599333
interactions of 2,4,6-trinitrotoluene (tnt) with xenobiotic biotransformation system in european eel anguilla anguilla (linnaeus, 1758).the aim of the present study was to investigate the interaction of 2,4,6-trinitrotoluene (tnt) with liver biotransformation enzymes in european eel anguilla anguilla (linnaeus, 1758). eels were exposed to 0.5, 1 and 2.5mg/l nominal concentrations of tnt for 6 and 24h. modulation of cyp1a1, udpgt and gst genes was investigated by real-time pcr. total cyp450 content, nadph cytochrome c reductase activity, cyp1a and cyp2b-like activities, such as erod, mrod and brod, as well as gst and udpgt activi ...200818407354
effects of 2,4,6-trinitrotoluene (tnt) on phase i and phase ii biotransformation enzymes in european eel anguilla anguilla (linnaeus, 1758).the aim of this study was to investigate effects of the explosive 2,4,6-trinitrotoluene (tnt) on liver drug metabolizing genes and enzymes in the european eel anguilla anguilla as a model fish species. eels were exposed in vivo for 6h and 24h to 0.5, 1 and 2.5mg/l nominal concentrations of tnt. expression of cyp1a, glutathione-s-transferase (pi-class; gst) and uridine-diphosphate glucuronosyltransferase (1-family) (udpgt) genes was investigated by rt-pcr, and 7-ethoxy- and 7-methoxyresorufin-o-d ...200818378298
pressure and temperature interactions on aerobic metabolism in migrating silver eels: results in vitro.the european eel (anguilla anguilla) migrates (6000 km) from european coast towards the supposed spawning area: the sargasso sea. this intensive and sustained swimming activity is performed without feeding and by using essentially red muscle i.e. aerobic metabolism. temperature and hydrostatic pressure vary during migration and have known effects on energy metabolism, mainly on mitochondrial functioning. we raise the question about the existence of a pressure-temperature combination that optimiz ...200818351124
is cl- protection against silver toxicity due to chemical speciation?in freshwater teleosts, the primary mechanism of acute silver toxicity is inhibition of na(+)/k(+) atpase and carbonic anhydrase at the gill, leading to net na(+) and cl(-) loss due to the continued diffusion of these ions into the hypoosmotic external environment. external cl(-) has been shown to protect rainbow trout (oncorhychus mykiss) against silver toxicity presumably by complexation to form agcl. however, cl(-) does not appear to greatly influence silver toxicity to at least two other spe ...200818304659
modulatory role of copper on β-naphthoflavone-induced dna damage in european eel (anguilla anguilla l.).gill and kidney dna integrity (alkaline unwinding assay) was assessed in anguilla anguilla exposed for 24-h to copper (cu: 1 or 2.5 μm), with or without 24-h pre-exposure to a polycyclic aromatic hydrocarbon (pah)-like compound--β-naphthoflavone (bnf: 2.7 μm). gill showed dna integrity loss in all the exposure conditions, reflecting a dual mode of bnf-cu interaction depending on the metal concentration. thus, antagonistic or additive effects were observed for bnf+cu 1 μm or bnf+cu 2.5 μm, respec ...200818304634
a field survey of metal binding to metallothionein and other cytosolic ligands in liver of eels using an on-line isotope dilution method in combination with size exclusion (se) high pressure liquid chromatography (hplc) coupled to inductively coupled plasma time-of-flight mass spectrometry (icp-tofms).the effect of metal exposure on the accumulation and cytosolic speciation of metals in livers of wild populations of european eel with special emphasis on metallothioneins (mt) was studied. four sampling sites in flanders showing different degrees of heavy metal contamination were selected for this purpose. an on-line isotope dilution method in combination with size exclusion (se) high pressure liquid chromatography (hplc) coupled to inductively coupled plasma time-of-flight mass spectrometry (i ...200818302969
first observations of histopathological effects of 2,4,6-trinitrotoluene (tnt) in gills of european eel anguilla anguilla (linnaeus, 1758): histopathological effects of 2,4,6-trinitrotoluene in gills of european eel.the aim of the present study was to investigate the effects on gill morphology of the explosive 2,4,6-trinitrotoluene (tnt) in a model fish, the european eel, anguilla anguilla (linnaeus, 1758) to assess potential detrimental effects in marine fish due to its presence in dumping areas. juvenile specimens of a. anguilla were exposed in vivo for 6 and 24 h to 0.5, 1 and 2.5 mg/l nominal concentrations of tnt using dimethyl sulfoxide (0.1 per thousand) as solvent carrier. histological analysis of g ...200818297406
isolation and characterization of 12 dinucleotide microsatellites in the european eel, anguilla anguilla l., and tests of amplification in other species of eels.twelve polymorphic dinucelotide microsatellites in the freshwater eel anguilla anguilla l. were isolated and characterized. genetic diversity was assessed in eels from lake constance, germany. allele numbers ranged from five to 26 per locus with observed heterozygosities between 0.125 and 0.875. a portion of locus aangct77 aligns with a transcribed region of the zebrafish gene crystallin beta b2. cross-species amplification of most markers was possible for nine other anguilla eel species. the ne ...200821586052
the inflammatory response of fish to helminth parasites.fish serve as a good model for studying vertebrate immune systems because they have a relatively simple system. descriptions of histopathological effects of helminth parasites on fish are few and far between with limited observations made on the identification of the inflammatory cells involved in the host reaction. recently, two cell types found within teleosts received a great deal of attention, namely mast cells and rodlet cells. fish most cells also known as eosinophilic granule cells, are m ...200818814717
glycolytic fluxes in european silver eel, anguilla anguilla: sex differences and temperature sensitivity.european silver eels migrate 6000 km to their supposed spawning area in the sargasso sea. as the eel is fasting, this intense swimming activity is realised only with fat stores, involving mainly red muscle i.e. aerobic metabolism. however, eel migration is performed at depth and thus in cold water, both being known to induce changes in muscle energy metabolism. during migration, white and red muscles can operate together or separately in order to counteract the eventual effects of low temperatur ...200818789394
biomonitoring of polybrominated diphenyl ether (pbde) pollution: a field study.polybrominated diphenyl ethers (pbdes) and cytochrome p450 enzyme activities were investigated in european eels (anguilla anguilla) collected from seven sites in a coastal lagoon in the north-western mediterranean sea, orbetello lagoon (italy). twelve pbde congeners were measured in muscle and two cyp1a enzyme activities, 7-ethoxyresorufin-o-deethylase (erod) and benzo(a)pyrene monooxygenase (bp(a)pmo), were investigated in liver microsomal fraction in order to obtain insights into the health of ...200818485829
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