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differential distribution and response to experimental sexual maturation of two forms of brain gonadotropin-releasing hormone (gnrh) in the european eel, anguilla anguilla.using specific radioimmunoassays for the two gnrh molecular forms present in the european eel, anguilla anguilla, (mgnrh and cgnrh ii), we compared their distributions in the pituitary and different parts of the brain of female silver eels, as well as the modifications of their levels in experimentally matured female eels (treated with carp pituitary extract). in control eels, mgnrh levels were higher than cgnrh ii levels in the pituitary, olfactory lobes and telencephalon, di- and mesencephalon ...199324202465
ascorbic acid transport by intestinal brush-border membrane vesicles of the teleost anguilla anguilla.transport of l-ascorbate by intestinal brush-border membrane vesicles of european eel anguilla anguilla was stimulated by a transmembrane na gradient (out > in) but not by a similarly directed gradient of k. under short-circuited membrane potential conditions, a kinetic analysis of l-ascorbate influx indicated the presence of a single na-dependent carrier process (kapp = 0.75 +/- 0.07 mm and jmax = 0.33 +/- 0.03 nmol.mg protein-1.min-1) and a nonsaturable transfer component with an apparent diff ...19938322981
the branching of insulin-like growth factor 1 and insulin: an immunohistochemical analysis during phylogeny.the co-existence of insulin-like growth factor 1 (igf-1) with the classical islet hormones insulin (ins), glucagon (gluc), somatostatin (som) and pancreatic polypeptide (pp) in the endocrine pancreas of representative species of cyclostomes (myxine glutinosa), cartilaginous fish (raja clavata, squalus acanthias) and bony fish (cottus scorpius, carassius auratus, cyprinus carpio, anguilla anguilla) was studied by the use of monoclonal and polyclonal antisera and the double immunofluorescence tech ...19938265818
effect of lipid content of diet on cholesterol content and cholesterogenic enzymes of european eel liver.the effect of dietary lipid levels on the levels of cholesterol and the activities of the major cholesterogenic enzymes of the liver has been studied in the european eel. an increase in hepatic total cholesterol was observed when the dietary lipid levels increased from 12 to 20%, while protein levels were maintained at 30%. this change paralleled an increase in mevalonate 5-pyrophosphate decarboxylase activity, while 3-hydroxy-3-methylglutaryl-coa reductase mevalonate kinase and mevalonate 5-pho ...19938246691
ultrastructural data on the sporogenesis of myxidium giardi, cépède 1906 (myxozoa, myxosporidia), parasite of anguilla anguilla (teleostea).ultrastructural study of myxidium giardi showed that pluricellularity is maintained by the formation of septate desmosomes. the polar capsule is formed from helicoidal depositions of banded material in the initial vacuole. this process makes possible not only the formation of the rigid capsular body but also of the flexible polar filament which is evaginable and reversed in the capsule. the special feature of the genus myxidium is the ornamentation of the valvogenic cells of the spores in the fo ...199323195549
importance of eating habits and sample size in the estimation of environmental mercury contamination using biological indicators.the importance of eating habits and the number of fish sampled in the estimation of mercury environmental contamination through biological indicators was studied. the species used were one with piscivorous habits (anguilla anguilla) and another with omnivorous habits (ciprinus carpio). from one original sample for each species, randomized samples were generated using the subroutine ggsrs from the mathematics library imsl, and later the statistical significance of pearson's correlation coefficien ...199324221024
synthesis of vitellogenin by eel (anguilla anguilla l.) hepatocytes in primary culture: requirement of 17 beta-estradiol-priming.control and 17 beta-estradiol-primed eels were used to investigate the hormonal requirement of vitellogenesis in an immature fish, the eel. a primary culture of isolated liver cells from female silver eels was developed. the hepatocytes were maintained as monolayers on poly-l-lysine coated dishes for up to 12 days in a defined medium alone or supplemented with 17 beta-estradiol (e2, from 10(-8) to 10(-5) m). the amounts of vitellogenin (vg) in the cells and secreted into the medium were measured ...19938224775
[cloning and sequence of thyrotropin beta subunit of a teleost fish: the eel (anguilla anguilla l.)].we obtained the sequence of eel thyrotropin beta-subunit cdna. about 1,100 bp long, it encodes a 147-amino acid protein including a 20-residue signal peptide. we analyse homologies between angtsh beta and the other known beta-subunits taking in account the ability of mammal gonadotropins (gth) to stimulate teleost thyroid. a peptide corresponding to mammalian cagyc (implicated in subunit association) is original and different from its eel beta gth2 counterpart.19938044698
developmental changes in the content of dopamine in the olfactory bulb of the european eel (anguilla anguilla).the concentrations of dopamine (da) and of its major metabolite, dihydroxyphenylacetic acid (dopac), were measured in discrete areas of the eel brain. to investigate the developmental changes in the content of da and dopac, the assays were performed in yellow eels (i.e. at the feeding stage) and silver eels (i.e. at the migratory stage). da and dopac were unevenly distributed in the eel brain. in yellow eels, the concentration of da was highest (16-19 pmol/mg protein) in the olfactory bulb (ob), ...19948084534
adrenergic control of swimbladder perfusion in the european eel anguilla anguilla.adrenergic control of swimbladder blood flow was analysed in blood-perfused preparations of the european eel as well as in situ by recording the changes in swimbladder blood flow and blood pressure following an injection of catecholamine into the dorsal artery. in blood-perfused swimbladder preparations, injection of the alpha-adrenergic agonist phenylephrine into the perfusion loop caused a marked dose-dependent increase in perfusion pressure at constant flow, while injection of the beta-agonis ...19947964385
uptake and clearance of diazinon in different tissues of the european eel (anguilla anguilla l.).acute toxicity effects of diazinon on european eel (anguilla anguilla) was examined using short-term exposures in static conditions. the lc50 values were 0.16, 0.11, 0.09 and 0.08 mg/l at 24, 48, 72 and 96 h exposure, respectively. in addition, the eels were exposed to 0.042 mg/l of diazinon and bioaccumulation and clearance processes of this insecticide in liver, muscle and gill tissues were studied. bioconcentration factor (bcf) of diazinon over 96 h exposure period was 1850 in liver, 875 in g ...19948024718
somatostatin-related peptides isolated from the eel gut: effects on ion and water absorption across the intestine of the seawater eel.four somatostatin-related peptides were isolated from eel guts. two of them were the same as eel ss-25ii (ess-25ii) and eel ss-25i (ess-25i) isolated from european eel pancreas. the remaining two peptides were c-terminal tetradecapeptides (ess-14ii and ess-14i) of ess-25ii and ess-25i, respectively. these four peptides all enhanced the serosa-negative transepithelial potential difference and short-circuit current across the seawater eel intestine after pretreatment with isobutylmethylxanthine, s ...19947525832
rapid and simultaneous measurement of anodic and cathodic haemoglobins and atp and gtp concentrations in minute quantities of fish bloodoxygen transport to vertebrate tissues is dependent upon (a) the intrinsic o2-binding properties of haemoglobin (hb) and (b) modulation by allosteric effectors, such as nucleoside triphosphates (ntp), that depress hb o2-affinity in fish red cells. while some species, such as flatfish, primarily use adenosine triphosphate (atp) to modulate o2-binding to hb, others, such as eel and carp, also use guanosine triphosphate (gtp) (kono and hashimoto, 1977; leray, 1982; weber and jensen, 1988). unlike m ...19949317794
ion transport in the intestine of anguilla anguilla: gradients and translocatorsthe transport of na+, k+ and cl- across the isolated epithelium of the eel intestine was studied using a combination of four experimental strategies: short-circuiting, measurements of intracellular potentials and ion concentrations, application of a variety of transport inhibitors and measurement of unidirectional fluxes with radioactive tracers. when short-circuited, the system performs a net transport of cl- and na+ towards the blood side, with a stoichiometry approaching 2, and a much smaller ...19949317418
induction of g-bands on anguilla anguilla chromosomes by the restriction endonucleases haeiii, hinfi, and msei.fixed metaphase chromosomes of the eel anguilla anguilla were treated with the restriction enzymes haeiii, hinfi, and msei. all three restriction endonucleases are capable of inducing a g-band-like pattern if the incubation time and enzyme concentration are controlled. the results are discussed in relation to the main factors involved in the appearance of serial g-bands. chromatin organization, rather than dna composition, seems to account for the interstitial g-band pattern exhibited by these e ...19948404070
the distribution of gaba-immunoreactive neurons in the brain of the silver eel (anguilla anguilla l.).the distribution of gaba-immunoreactivity was studied in the brain of the silver eel (anguilla anguilla) by means of antibodies directed against gaba. immunoreactive neuronal somata were distributed throughout the brain. positive perikarya were detected in the internal cellular layer of the olfactory bulb, and in all divisions of the telencephalon, the highest density being observed along the midline. numerous gaba-reactive cell bodies were found in the diencephalon, particularly in the preoptic ...19948192235
the size and number of neurons descending to the spinal cord in relation to body length in the european eel (anguilla anguilla).cells of origin of descending spinal pathways in eels of different body lengths (15-59 cm) were identified by labelling them retrogradely with horseradish peroxidase from the rostral spinal cord. the aim was to determine whether changes in size, number and distribution of these neurons take place in line with changes in the size of the spinal targets that occur with increasing body length. the somal cross-sectional areas of the labelled cells were measured from horizontal frozen sections and cou ...19948082024
an 8-year study on the elimination of pcbs and other organochlorine compounds from eel (anguilla anguilla) under natural conditions. 199422176041
a new mannan-binding lectin from the serum of the eel (anguilla anguilla l.): isolation, characterization and comparison with the fucose-specific serum lectin.a new mannan-binding lectin (mbl) and a previously described fucose-binding lectin (fbl) have been isolated from serum of anguilla anguilla by affinity chromatography on a-peptone-sepharose in combination with electroelution (mbl) or affinity chromatography using alpha-l-fucose-agarose (fbl). mbl has a mol. wt of approximately 246,000 and is composed of identical subunits of approximately 24,000, two of each are always covalently linked. fbl has a mol. wt of about 121,000 and consists of four su ...19947953065
sterol and fatty acid composition of neutral lipids of paratenuisentis ambiguus and its host eel.the sterol composition of free sterol and steryl ester fractions of the fish parasite paratenuisentis ambiguus was determined. in addition, the fatty acid composition of various neutral lipid classes, i.e., wax esters, steryl esters, triacylglycerols and free fatty acids, as well as the composition of the 1-o-alkyl moieties of total ether glycerolipids of the parasite, were investigated. the results of these studies were compared with those obtained on the intestinal tract tissue of its host, th ...19948090063
sequence and regulation of european eel prolactin mrna.cdna clones encoding the european eel (anguilla anguilla l.) prolactin were isolated from a pituitary cdna library constructed in gamma gt10, using a rainbow trout prl cdna fragment as a probe. four different inserts were subcloned into the pgem 3z plasmid after pcr amplification. the 1082 bp-long nucleotide sequence revealed an open reading frame of 627 bp encoding a 24 amino acid-long signal peptide followed by a 185 amino acid-long mature protein. comparison studies showed 60-70% homology wit ...19947926267
in vitro dose-response study of the effect of cadmium on eel (anguilla anguilla) gill na+/k(+)-atpase activities.the effect of cadmium exposure was studied in vitro on the atpase activities of gill membrane microsomes from seawater- and freshwater-adapted eel (anguilla anguilla) using a microassay technique. the basal activity (mg(2+)-atpase) was decreased by 40 and 25%, respectively, in seawater and freshwater preparations for the highest concentrations tested (respectively, 4 and 2 microm). the na+/k(+)-atpase activity was estimated either by potassium stimulation or by ouabain inhibition. this enzyme ac ...19947523067
angiotensin ii receptor subtypes in eel (anguilla anguilla).previous studies have shown the effects of angiotensin ii (ang ii) in teleosts, and ang ii-binding sites have also been localized in tissues from rainbow trout. the purpose of this study was to extend these findings and to provide an analysis of ang ii receptor (ang ii-r) isoforms in three tissues obtained from european eel (anguilla anguilla). ang ii-rs were identified in eel liver, kidney and intestine membranes by the binding of either 0.5 nmol human 125i-labelled tyr4-ile5-ang ii/l or increa ...19948185815
some aspects of zinc transport across eel (anguilla anguilla) red blood cell membranes.zinc movement across eel and human red blood cell membranes was measured by atomic absorption spectrophotometry. it was observed that: 1) in human red blood cells zinc uptake is twice as rapid as in fish red blood cells over a temperature range of 10-40 degrees c. the low rate of zinc uptake in eel red blood cell may be simply the side effect of different surface area to volume ratios for the differences in cell size or, it may be due to the low permeability of bicarbonate through the red blood ...19948044143
two pathways for choline transport in eel erythrocytes: a saturable carrier and a volume-activated channel.choline transport in eel (anguilla anguilla) erythrocytes was investigated in cells suspended in isotonic and hypotonic media. in cells in isosmotic solution choline transport was mediated by a saturable system with a michaelis constant (km; 62 +/- 6 microm) similar to that of the choline carrier of human erythrocytes but a maximal transport rate (vmax; 4.5 +/- 0.4 mmol.1 red blood cells-1.h-1) almost two orders of magnitude higher than that in human red blood cells. this pathway was inhibited b ...19948092322
immunocytochemical localization of mammalian gnrh (gonadotropin-releasing hormone) and chicken gnrh-ii in the brain of the european silver eel (anguilla anguilla l.).using specific antibodies for the two molecular forms of gonadotropin-releasing hormone (gnrh) present in the european eel, anguilla anguilla, (mammalian gnrh, mgnrh, and chicken gnrh ii, cgnrh-ii), we employed immunocytochemistry to determine the distribution of these two peptides in the brain and in the pituitary. the results indicate that mgnrh and cgnrh-ii are localized in different neurons: mgnrh-immunoreactive (ir) perikaria were observed in the olfactory bulbs, the junction between olfact ...19947873095
present occurrence of anguillicola novaezelandiae (nematoda: dracunculoidea) in europe and its development in the intermediate host.an examination of a sample of european eels, anguilla anguilla (l.), collected from lake bracciano near rome in 1993, the only known european locality with the occurrence of the introduced swimbladder nematode anguillicola novaezelandiae moravec et taraschewski, 1988, revealed for the first time the presence of two anguillicola species, a. novaezelandiae and a. crassus. in view of the investigations carried out by current authors in bracciano lake in the years 1982-1992, it is apparent that the ...19947883252
third-stage larvae of daniconema anguillae (nematoda: dracunculoidea) in the subcutaneous tissue of eel anguilla anguilla.daniconema anguillae moravec et køie, 1987 larvae measuring 1.64-1.76 mm were occasionally found in considerable numbers in the fins and subcutaneous connective tissue of approximately 50% of eel anguilla anguilla (l.) sampled from lake balaton, hungary. the larvae were noted for their slender body, very long tail with a rounded tip, a densely transversely striated cuticle, and the presence of boring tooth and large kidney-shaped amphids on the cephalic end. the larvae could easily be recovered ...19947883254
white muscle differentiation in the eel (anguilla anguilla l.): changes in the myosin isoforms pattern and atpase profile during post-metamorphic development.myosin isoforms and their light and heavy chains subunits were studied in the white lateral muscle of the eel during the post metamorphic development, in relation with the myosin atpase profile. at elver stage vi a1 the myosin isoforms pattern was characterized by at least two isoforms, fm3 and fm2. the fast isomyosin type 1 (fm1) appeared during subsequent development. it increased progressively in correlation with the increase in the level of the light chain lc3f. fm1 became predominant at sta ...19941534545
dynamics of anguillicola crassus (nematoda: dracunculoidea) larval infection in paratenic host fishes of lake balaton, hungary.anguillicola crassus, a nematode parasitizing the swimbladder of the european eel (anguilla anguilla) caused substantial mortality among eels in lake balaton in the years 1991 and 1992. parallel to a 3-year study of the infection dynamics of eels, anguillicola larval infection of paratenic host fishes was also surveyed in the lake between 1991 and 1993. during that study, a total of 1,382 specimens of 22 paratenic host fish species were processed. the results showed that anguillicolosis had beco ...19958882739
dna dosimetry in biological indicator species living on pah-contaminated soils and sediments.a large variety of environmental carcinogens are metabolically activated to electrophilic metabolites that can bind to nucleic acids, forming covalent adducts. in organisms possessing active metabolic systems for a particular carcinogen, dna adducts generally have longer biological half-lives than the substrate carcinogens. thus, measurement of specific dna adduct concentrations in terrestrial and water organisms may provide a relevant biological indicator of prior exposure to environmental carc ...19957539372
differential regulation of the two forms of gonadotropin-releasing hormone (mgnrh and cgnrh-ii) by sex steroids in the european female silver eel (anguilla anguilla).the effect of steroids on the two gonadotropin-releasing hormone (gnrh) forms present in the eel (mammalian gnrh, mgnrh and chicken gnrh-ii, cgnrh-ii), as well as on gonadotropin (gth), was studied using specific radioimmunoassays. female silver eels received chronic treatments with various steroids (estradiol, testosterone, androstenedione, 5 alpha-androstane-3 beta, 17 beta-diol). estradiol or the combination of estradiol and androgens induced increases in brain and pituitary mgnrh levels and ...19957617130
the cathodic hemoglobin of anguilla anguilla. amino acid sequence and oxygen equilibria of a reverse bohr effect hemoglobin with high oxygen affinity and high phosphate sensitivity.as in other fish, the cathodic hemoglobin of the eel anguilla anguilla is considered to play an important role in oxygen transport under hypoxic and acidotic conditions. in the absence of phosphates this hemoglobin shows a reverse bohr effect and high oxygen affinity, which is strongly modulated over a side ph range by gtp (whose concentration in the red blood cells varies with ambient oxygen availability). gtp obliterates the reverse bohr effects in the cathodic hemoglobin. the molecular basis ...19957642546
primary sequence, tissue specificity and expression of the na+,k(+)-atpase alpha 1 subunit in the european eel (anguilla anguilla).the entire cdna nucleotide sequence of the na+,k(+)-atpase alpha 1 isoform was cloned from the gills of the european eel (anguilla anguilla) by a pcr based method. the amino acid sequence translated from the sequence shared 89.4 and 85.6% homology respectively with previously published na+,k(+)-atpase alpha subunit sequences from elasmobranch (torpedo californica) and teleost (catostomus commersoni) fish. the size of na+,k(+)-atpase alpha 1 mrna transcripts in eel tissues was demonstrated to be ...19958574922
characterization of plasma membrane na+/h+ exchange in eel (anguilla anguilla) intestinal epithelial cells.the ability of eel intestinal epithelial cells to recover from an acute acid load was analysed using the fluorescent dye 2',7'-bis-carboxy-ethyl-5,6-carboxyfluorescein (bcecf) and cell suspensions. under these experimental conditions (bicarbonate-free solutions) the resting phi in cells prepared from sea-water (7.52 +/- 0.031) and fresh-water (7.50 +/- 0.094) adapted animals proved to be similar. the recovery rate (following an acid load) increases by increasing the na ion concentration in the e ...19957852945
colocalization of (ttaggg)n telomeric sequences and ribosomal genes in atlantic eels.the distribution of (ttaggg)n telomeric repeats was studied in chromosomes of two atlantic eels, anguilla anguilla and a. rostrata. we found that these sequences hybridize to all the telomeres but also to the entire nucleolar organizer region (nor) localized in both species at the short arm of chromosome 8. this was considered to be due to the interspersion of telomeric sequences within the nor ones. whatever the significance of this interspersion may be, it seems to be limited to a. anguilla an ...19957704417
[anguilluliasis of the european eel--a continuing problem]. 19957571636
[effects of hydrostatic pressure on malondialdehyde (mda) determination in brain from yellow freshwater eels].malondialdehyde (mda) content in a tissue can be an indicator of lipoperoxidation and thus of membrane alteration. mda concentrations have been measured in the brain of yellow freshwater eels (anguilla anguilla) submitted to 1.51 or 101 ata of hydrostatic pressure. exposure to pressure was performed for 6 h at constant water temperature and in normoxic conditions. the results thus obtained show a significant increase (p < 0.05 or better) of mda brain concentrations at 51 ata (+ 124%) and 101 ata ...19957583764
immunohistochemical localization of bioactive peptides and amines associated with the chromaffin tissue of five species of fish.biogenic peptides and amines associated with the chromaffin tissue in atlantic cod (gadus morhua), rainbow trout (oncorhynchus mykiss), european eel (anguilla anguilla), spiny dogfish (squalus acanthias) and atlantic hagfish (myxine glutinosa) were identified utilizing immunohistochemical techniques. within the posterior cardinal vein (pcv) in cod, trout and eel, a subpopulation of chromaffin cells displayed immunoreactivity to tyrosine hydroxylase (th) and dopamine-beta-hydroxylase (d beta h) b ...19957606765
effect of hco-3 and ph on ion transport in the posterior intestine of the freshwater-adapted teleost anguilla anguilla.the intestinal mucosa may be exposed to acidic or alcaline solutions due to liberation of digestive secretions. in several situations blood ph may also change. consequently, the effects of hco-3, co2, and ph variation of medium on the ion transport across the posterior intestine of the eel (anguilla anguilla) adapted to freshwater were studied in terms of fractional values of short-circuit current (scc), transepithelial potential difference (tpd) and conductance (g). immature eels weighing 100-2 ...19958520526
production, characterisation and applicability of monoclonal antibodies to european eel (anguilla anguilla l., 1758) immunoglobulin.monoclonal antibodies (mabs) to european eel (anguilla anguilla l., 1758) immunoglobulin (ig) were produced, characterised and tested for applicability in a number of immuno(cyto)chemical assays. the selected mabs, wei 1 and wei 2, were specifically reactive with ig heavy and light chain, respectively. wei 1 appeared to react with all or nearly all ig molecules, b cells and plasma cells. wei 2 was reactive with a subpopulation of those cells, indicating that european eel possesses at least two a ...19957604532
genome size and at content in anguilliformes.the genome sizes (gs) and at contents of five species of anguilliformes (anguilla anguilla, anguilla rostrata, conger conger, gymnothorax unicolor and muraena helena) belonging to three families (anguillidae, congridae and muraenidae) were determined by flow cytometry and densitometry. differences in gs between anguillidae-congridae (about 3 pg) and muraenidae (about 5 pg) were found, which correlate with the relative amounts of at base pairs (around 42-43% for anguillidae-congridae and 51-52% f ...19958835179
the molecular basis for the green-blue sensitivity shift in the rod visual pigments of the european eel.when the european eel matures sexually and migrates back to deep sea breeding grounds the visual pigments in its rod photoreceptors change from being maximally sensitive to green light to being maximally sensitive to blue light. in part, this change in sensitivity is due to a change in the opsin component of the visual pigment molecule. we used hormone injection to induce these developmental changes in a group of eels and from these animals an opsin coding region was cloned and sequenced using c ...19958587887
effects of a temperature increase on oxygen consumption of yellow freshwater eels exposed to high hydrostatic pressure.oxygen consumption was measured in yellow freshwater eels, anguilla anguilla, during exposure to a hydrostatic pressure (hp) of 101 atmosphere absolute (ata) for 3 h, either with or without a 5 degrees c increase in water temperature. it was found that concomitant increases in temperature and pressure resulted in an increase in oxygen consumption that was lower than when pressure alone was increased. when eels were acclimated to hp for 21 days and then submitted to a temperature increase of 5 de ...19958962704
primary sequence, tissue specificity and mrna expression of the na(+),k (+) -atpase β1 subunit in the european eel (anguilla anguilla).the entire amino acid coding sequence of the na(+),k(+)-atpase β1 isoform was cloned from the gill of the european eel (anguilla anguilla) by a pcr based method. the amino acid sequence translated from the nucleotide sequence shared 61.4 and 56.2% homology respectively with previously published na(+),k(+)-atpase β1 isoform sequences from the clawed toad (xenopus laevis) and the ray (torpedo californica) an elasmobranch fish. the size of the na(+),k(+)-atpase β1 mrna transcript in eel tissues was ...199524197533
the role of the renin-angiotensin system in the control of blood pressure and drinking in the european eel, anguilla anguilla.the role of the renin-angiotensin system (ras) in the control of blood pressure and drinking was investigated in fresh water (fw)- and seawater (sw)-adapted eels, anguilla anguilla, by comparing the effects of pharmacological manipulation through the use of papaverine (stimulator) and captopril (inhibitor) on the endogenous system. in sw eels basal blood pressure levels were lower (23.3 +/- 0.8 mm hg) with correspondingly higher basal drinking rates (0.51 +/- 0.07 ml/kg/hr) and plasma aii concen ...19958575657
mechanisms of acid release in isolated gas gland cells of the european eel anguilla anguilla.mechanisms of acid production and of acid release have been analyzed in isolated gas gland cells of the eel swimbladder using a cytosensor microphysiometer. incubation of isolated cells with oxamic acid caused a dose-dependent decrease in the rate of proton release. at the highest oxamic acid concentration used (20 mmol/l), proton release was reduced by approximately 40%; incubation with sodium fluoride (10 mmol/l) or removal of glucose from the extracellular medium caused 60 and 80% reduction, ...19957485595
influence of flumequine on in vivo mitogen responses of european eel (anguilla anguilla l., 1758) lymphoid cells.the influence of flumequine on mitogen induced lymphoid cell proliferation in european eels (anguilla anguilla l., 1758) was studied. for this purpose an in vivo test, using peroral drug administration followed by successive intraperitoneal injections with concanavalin a (cona) or bacterial lipopolysaccharides (lps) and 5-bromo-2'-deoxyuridine, was applied. direct counting of proliferated cells in blood smears revealed that flumequine possesses mitogenic properties. a synergistic and an antagoni ...19958533292
pesticide effects on eel metabolism pesticide; levels did not decline at any time when animals were exposed to 4.1 mg/liter.previous works on endosulfan eel toxicology in this laboratory demonstrated that 0.041 mg/liter of endosulfan was the 50% lethal concentration of 96 hr exposure. eels of species anguilla anguilla were exposed to two sublethal endosulfan concentrations: 8.2 micrograms/liter (1/5 lc50) and 4.1 micrograms/liter (1/10 lc50), and the experiment was done at different exposure times: 12, 24, 48, 72, and 96 hr. muscle glycogen content decreased significantly (p < 0.05) at 24, 48, 72, and 96 hr exposure ...19958521781
a monoclonal antibody to mammalian angiotensin ii at1 receptor recognizes one of the angiotensin ii receptor isoforms expressed by the eel (anguilla anguilla).using labelled ligand-binding methods, previous studies have identified specific angiotensin ii receptors (ang ii-rs) in eel liver, kidney and intestine membranes. isoelectric focusing on polyacrylamide gels also showed that there are two ang ii-r isoforms in eel liver, focusing at isoelectric points (pi) 6.5 and 6.7. these may have different functions. in contrast, eel enterocyte plasma membrane and renal brush border membranes contain only the pi 6.5 form. to characterize the eel receptors mor ...19968672232
the erythrocyte na+/h+ exchangers of eel (anguilla anguilla) and rainbow trout (oncorhynchus mykiss): a comparative studytrout and eel red blood cell na+/h+ exchangers show widely different regulatory properties. catecholamines, cyclic amp and phorbol esters, which activate the trout red cell antiporter, do not affect the eel exchanger. unlike the trout red cell exchanger, the eel red cell exchanger is strongly activated by cell shrinkage, allowing a remarkable cell volume recovery. these different regulatory properties probably indicate the existence of different isoforms of the exchangers in nucleated erythrocyt ...19969318046
mediation of serotonin-induced hyperventilation via 5-ht3-receptor in european eel anguilla anguilla.the effects of serotonin (5-hydroxytryptamine) on ventilation were investigated by continuous measurements of intrabuccal pressure in unrestrained eel. intravenous administration of 5-hydroxytryptamine (30 micrograms.kg-1) caused a large increase in ventilatory frequency (+ 100%) and amplitude (+ 140%). the 5-hydroxytryptamine-induced hyperventilation was blocked by the 5-ht3-receptor antagonists metoclopramide (1.0 mg.kg-1) or mdl72222 (1.0 mg.kg-1), and was insensitive to the 5-ht1/2-receptor ...19968882510
physiological stress responses of anguilla anguilla to fenitrothion.the present communication deals with the effect of fenitrothion (0.04 ppm) on the carbohydrate metabolism of the european eel anguilla anguilla and its recovery from intoxication. analysis of various parameters such as glycogen, lactate and glucose was made on different eel tissues after 2, 8, 12, 24, 32, 48, 56, 72 and 96 hr of fenitrothion exposure. subsequently, the fish were allowed recovery periods of 8, 12, 24, 48, 72, 96, 144 and 192 hr in clean water, and the same parameters were evaluat ...19968847448
swelling-activated k+ transport via two functionally distinct pathways in eel erythrocytes.following osmotic swelling, erythrocytes from the european eel, anguilla anguilla, underwent a regulatory volume decrease. this was prevented by replacement of na+ with k+ in the suspending medium, consistent with a role for the (normally outward) electrochemical k+ gradient in the volume-regulatory response. the effect of cell swelling on k- transport in these cells was investigated using 86rb+ as a tracer for k+. osmotic swelling resulted in an increase in ouabain-insensitive k+ transport that ...19968769785
chromosomal localization of the major and 5s rrna genes in the european eel (anguilla anguilla).the major (18s, 5.8s, and 28s) and 5s rrna genes have been mapped by double fluorescent in situ hybridization to european eel metaphase chromosomes. the major rrna genes were localized to a submetacentric pair of chromosomes that showed a consistent size polymorphism among the individuals studied. the 5s rrna genes were clustered in a single locus that mapped to the centromeric region of an acrocentric pair. in contrast to the major rrna genes, no detectable polymorphism, in either size or inten ...19968646885
effects of diazinon exposure on cholinesterase activity in different tissues of european eel (anguilla anguilla).cholinesterase (che) activity was measured in brain, plasma, and whole eye of anguilla anguilla experimentally exposed to a sublethal concentration of 0.042 mg/liter (0.50 of the 96-hr lc50) of the organophosphorous pesticide diazinon. whole eye was the tissue which revealed higher values of che activity (8.17 micromol/min/g) in nonexposed animals. brain, plasma, and whole eye che activity of a. anguilla was inhibited at 6, 24, 48, 72, and 96 hr of diazinon exposure. pesticide induced significan ...19969006996
anguilla anguilla l. stress biomarkers recovery in clean water and secondary-treated pulp mill effluent.the eels' recovery from capture, anoxia, and transport is improved by the dechlorinated filtered aerated tap water temperature increase and increased water salinity. the eels' 6-hr recovery from capture, anoxia, and transport, at 25 degrees c in 50% secondary-treated bleached kraft pulp mill effluent, as plasma lactate and cortisol uptake, is depressed. however, either farm or wild eels' exposure to the 50% secondary-treated bleached kraft pulp mill effluent, after 188 hr recovery from capture, ...19968930510
bicarbonate absorption in eel intestine: evidence for the presence of membrane-bound carbonic anhydrase on the brush border membranes of the enterocyte.bicarbonate absorptive fluxes through the isolated intestine of the european eel (anguilla anguilla) were evaluated by the ph-stat method under short-circuited conditions. it was found that bicarbonate absorptive flux was dependent on the luminal na+ and was inhibited by luminal 4-acetamido-4' stilbene-2-2' disulfonic acid (sits; 2.5 x 10(-4) m) and luminal acetazolamide (10(-4) m), while luminal amiloride (1 mm) was without effect. furthermore, by using brush border membrane vesicles (bbmv) iso ...19968691189
absorbance spectra and molecular structure of the blue-sensitive rod visual pigment in the conger eel (conger conger).the conger eel (conger conger) is a nocturnal fish that can be found living in shallow coastal water and deep water down to 1000 m. the conger eel has a pure rod retina with a visual pigment maximally sensitive to blue light around 487 nm. we have cloned and sequenced the opsin cdna which is presumed to code for this visual pigment and have found it to be highly homologous to the form of opsin that is expressed in mature, deep-living european eels (anguilla anguilla). the opsin sequence informat ...19968763796
changes in enzyme histochemical profiles of identified spinal motoneurons of the european eel, anguilla anguilla, following cordotomy.the enzyme histochemical profiles of glucose-6-phosphate dehydrogenase (a marker of synthetic performance), succinate dehydrogenase (an indicator of oxidative metabolism), and nadh tetrazolium reductase (a marker of overall neuronal activity) were determined for identified white muscle motoneurons in six control and six cordotomized cels. images were digitized and mean integrated absorbances obtained using appropriate hardware and software. for motoneurons caudal to the transection site there wa ...19968818680
control of acid secretion in cultured gas gland cells of the european eel anguilla anguilla.single cells and cell clusters isolated from the swimbladder epithelium of the european eel anguilla anguilla attached to collagen s-coated petri dishes and proliferated in a modified dulbecco's modified eagle's medium, supplemented with 0.5% fetal calf serum. at a temperature of 20-22 degrees c, the growing colonies reached confluence typically within 6-8 days. activities of glycolytic and pentose phosphate shunt enzymes remained stable or increased only slightly during the first 10 days of pri ...19968780223
effects of serotonin on the cardio-circulatory system of the european eel (anguilla anguilla) in vivo.the effects of serotonin on continuously recorded cardiac parameters (heart rate, cardiac output, cardiac stroke volume), ventral and dorsal aortic blood pressures, branchial and systemic vascular resistances were investigated in the european eel in vivo. intravenous administration of serotonin (30 micrograms.kg-1) caused a marked bradycardia (45%) and a simultaneous decrease in cardiac output (50%), ventral (35%) and dorsal (50%) aortic blood pressures. branchial resistance was markedly increas ...19968766910
fluorescence-labeled fucolectins are superior markers for flow cytometric quantitation of the human sperm acrosome reaction.to evaluate the binding of three fluorescein (fitc)-labeled fucose-specific lectins, anguilla anguilla agglutinin (aaa), tetragonolobus purpureas agglutinin (tpa), and ulex europaeus-1 agglutinin (uea-1), to unfixed, acrosome-intact and acrosome-reacted (ar) human sperm by flow cytometry and to compare the results with those found using fitc-labeled pisum sativum agglutinin (psa) and arachis hypogaea agglutinin (pna).19968654649
angiotensin ii stimulation of the basolateral located na+/h+ antiporter in eel (anguilla anguilla) enterocytes.the ph-sensitive fluorescent dye, 2',7'-bis-carboxyethyl-5, 6-carboxyfluorescein acetoxymethyl ester, was used to examine the effects of fish or human angiotensin ii (ang ii) on the activity of the basolateral located na+/h+ antiporter in eel intestinal cell suspensions. exposure of eel enterocytes to either hormone led to an increased activity of the antiporter. this time- and dose-dependent stimulatory effect was inhibited by the specific antiporter inhibitor dimethylamiloride (dma). preincuba ...19968672233
genomic variation of aquatic birnaviruses analyzed with restriction fragment length polymorphisms.aquatic birnaviruses are the most ubiquitous and diverse group of viruses in the family birnaviridae. several cause different diseases in a variety of fish species, such as infectious pancreatic necrosis virus in salmonids in north america, europe, and asia and european eel virus in eel in asia. most isolates are antigenically related and belong to a single serogroup (serogroup a) comprising nine serotypes. previous studies with monoclonal antibodies have demonstrated considerable variation in e ...19968779591
influence of flumequine and oxytetracycline on the resistance of the european eel against the parasitic swimbladder nematode anguillicola crassus.the effect of the antibacterial drugs flumequine (fq) and oxytetracycline (otc) on the defence system of the european eel (anguilla anguilla l., 1758) was investigated using an experimentally induced infection with the parasitic swimbladder nematode anguillicola crassus. eight weeks after oral administration of infective larvae, the mean recovery of the parasites in fq-treated eels was lower than in non-medicated control animals, and significantly lower than in otc-treated eels. mean numbers of ...19968807782
localization of rdna genes in european eel (anguilla anguilla) by fish.a probe containing most of the transcription unit of the major ribosomal genes of xenopus laevis has been used to localize the rdna cluster of european eel (anguilla anguilla) by fluorescence in situ hybridization (fish). only on nor-bearing chromosome pair has been found. the results obtained with this technique confirm the size polymorphism of the nor revealed previously by other classical cytogenetic techniques.19968983192
colonization and establishment of pomphorhynchus laevis (acanthocephala) in an isolated english river.the successful colonization, establishment and spread of pomphorhynchus laevis in a small, isolated, devon river 128 km from the parasite's nearest focus in dorset was followed over 11 years from 1985. the parasite was first detected in anguilla anguilla and platichthys flesus in 1988: by 1995 it had attained prevalence levels of 22.6% in a. anguilla and 43.6% in p. flesus and also occurred in 100% salmo trutta, 50% cottus gobio and noemacheilus barbatulus. as judged by prevalence, abundance, pr ...19968991914
different effects of cgmp and camp in the intestine of the european eel, anguilla anguilla.the regulation of salt absorption in the sea water eel intestine was studied by evaluating the effects of theophylline, 8 br cyclic adenosine monophosphate, 8 br cyclic guanosine monophosphate, atriopeptin iii, porcine vasoactive intestinal peptide and prostaglandin e1 on the short-circuit current, the transepithelial voltage difference and conductance and on the dilution potentials. it was shown that theophylline increased the transepithelial conductance and reduced the magnitude of the dilutio ...19968621839
characterization of the binding specificity of anguilla anguilla agglutinin (aaa) in comparison to ulex europaeus agglutinin i (uea-i).using immunochemical and immunohistochemical methods, the binding site of anguilla anguilla agglutinin (aaa) was characterized and compared with the related fucose-specific lectin from ulex europaeus (uea-i). in solid-phase enzyme-linked immunoassays, the two lectins recognized fuc alpha 1-2gal beta-hsa. aaa additionally cross-reacted with neoglycolipids bearing lacto-n-fucopentaose (lnfp) i [h type 1] and ii [le(a)] and lactodifucotetraose (ldft) as glycan moieties. uea-i, on the other hand, bo ...19968872115
eel (anguilla anguilla) and brown trout (salmo trutta) target species to assess the biological impact of trace metal pollution in freshwater ecosystems.copper, lead, and cadmium pollution were measured in water and sediments of two northern spanish rivers: piles and pigäeña. liver contents of these heavy metals were analyzed in two fish species (eel, anguilla anguilla, and brown trout, salmo trutta) collected from the same locations. significant levels of heavy metal pollution were found in a 38% of fish that are potential catches for sport fishermen. the results indicate that adult eel could be a good metal bioindicator if sampled at a homogen ...19968854823
changes in gas composition in the swimbladder of the european eel (anguilla anguilla) infected with anguillicola crassus (nematoda).the effect of anguillicola crassus (nematoda) on gas composition in the swimbladder of eels (anguilla anguilla) was studied using mass spectrometry in feral eels from two german rivers and in experimentally infected eels. in both naturally and experimentally infected eels significant correlations were observed between the proportion of oxygen in the swimbladder and level of infection with anguillicola crassus. in swimbladders of naturally infected feral eels the contribution of oxygen to swimbla ...19968851864
primary cultures of dispersed pituitary cells from estradiol-pretreated female silver eels (anguilla anguilla l.): immunocytochemical characterization of gonadotropic cells and stimulation of gonadotropin release.to analyze the multihormonal control mechanisms of gth secretion in the eel, primary culture of pituitary cells from control or estradiol-treated female silver eels, a treatment known to stimulate in vivo gth synthesis, was developed. dispersed eel pituitary cells obtained by enzymatic (trypsin/dnase) and mechanical dispersion were cultured in earles m199, at 18 degrees. immunoreactive gth (ir-gth) cells were characterized by the immunoperoxidase method, using antiserum to carp gth beta subunit. ...19968921361
production of recombinant insulin-like growth factor-ii in the development of a radioimmunoassay in rainbow trout (oncorhynchus mykiss).rainbow trout (oncorhynchus mykiss) insulin-like growth factor-ii (igf-ii) cdna was amplified by reverse transcription-polymerase chain reaction and cloned into an expression vector (parhs3) for production by escherichia coli. recombinant rainbow trout igf-ii (rtigf-ii) was recovered from bacterial inclusion bodies, solubilized, and purified by gel filtration chromatography under reducing conditions. after refolding of the peptide by dialysis at basic ph, we obtained a final yield of 820 microgr ...19968930606
potentiating effect of growth hormone on vitellogenin synthesis induced by 17 beta-estradiol in primary culture of female silver eel (anguilla anguilla l.) hepatocytes.previous in vivo experiments have indicated a potentiating role of growth hormone (gh) during experimentally induced vitellogenesis by 17-beta-estradiol (e2) in the female silver eel (anguilla anguilla l.). to investigate whether gh has direct hepatic actions, the effects of hypophysial-purified and recombinant qh on vitellogenin (vg) synthesis in response to e2 were tested on primary cultures of hepatocytes. hepatocytes were prepared from control or e2-primed eels. addition of e2 alone into the ...19968998971
purification and characterization of the tricarboxylate carrier from eel liver mitochondria.the tricarboxylate carrier from eel (anguilla anguilla) liver mitochondria was solubilized with triton x-100 and purified by sequential chromatography on hydroxyapatite and matrex gel blue b. on sds-polyacrylamide gel electrophoresis, the purified fraction showed a single polypeptide band with an apparent molecular mass of 30.4 kda. when reconstituted into liposomes, the tricarboxylate transport protein catalyzed a very active 1,2,3-benzenetricarboxylate-sensitive citrate/citrate exchange. it wa ...19968687426
importation of aquaculture seedlings to japan.japan is one of the largest consumers of fishery products in the world. importation of various fishery commodities - including larvae and eggs for aquaculture - is increasing due to high consumer demand. elvers of anguilla anguilla have been imported from europe since the late 1960s. eyed eggs of coho salmon (oncorhynchus kisutch) are imported from north america. during the last decade, over-production caused the price of japanese amberjack (or 'yellowtail') (seriola quinqueradiata) and japanese ...19968890372
development of a radioimmunoassay for european eel growth hormone and application to the study of silvering and experimental fasting.a specific and sensitive homologous radioimmunoassay for eel (anguilla anguilla l.) growth hormone (anggh) has been developed. the antiserum, raised against purified anggh and used at 1:20,000 final dilution, did not cross-react with eel prolactin or thyrotropin, carp gonadotropin ii, bovine gh, or serum from hypophysectornized eel. the inhibition curves for eel pituitary extracts and serum were parallel to that of anggh standard. the ed50 value was between 1 and 2 ng/tube and the recovery of pu ...19968804566
bioconcentration and excretion of fenitrothion in the brain of the eel (anguilla anguilla).the bioconcentration of fenitrothion in the brain of the european eel (anguilla anguilla) and its posterior elimination have been studied. animals were exposed to a sublethal concentration of fenitrothion (0.04 mg/l) for 96 hours in a flow-through test system. after this pesticide exposure, animals were transferred to clean water for 72 hours more. bioconcentration and elimination processes of fenitrothion were studied in blood and brain. this insecticide showed a strong tendency to bioconcentra ...19979350078
similarities and differences between the massive eel (anguilla anguilla l.) devastations that occurred in lake balaton in 1991 and 1995.in the past few years, two massive eel (anguilla anguilla l.) devastations occurred in lake balaton, hungary. in 1991, 300 tons of eel perished in the western basin of the lake, while in the summer of 1995 30 tons of eel died in the eastern part of the lake. investigations carried out to find the causes of these ecocatastrophes included measurements of certain biochemical parameters: the blood sugar level, and the acetylcholinesterase (ache, ec 3.1.1.7), lactate dehydrogenase (ldh, ec 1.1.2.3), ...19979212331
endoparasitic helminths of the european eel, anguilla anguilla, from four disconnected meanders from the rivers leie and scheldt in western flanders, belgium.the endoparasitic helminth communities of the european eel, anguilla anguilla (l.), were investigated in four meanders, cut off from the rivers leie and scheldt in western flanders, belgium. six species of helminths (2 cestodes, 2 nematodes and 2 acanthocephalans) were found. the dominant parasite species was the nematode anguillicola crassus (kuwahara, niimi et itagaki, 1974) infecting 79% of the eel population with intensities up to 112 specimens per fish. at two localities no acanthocephalans ...19979229569
down-regulation of tsh subunit mrna levels by thyroid hormones in the european eel.the effects of 3,5,3'-triiodothyronine (t3) and thyroxine (t4) on alpha and thyroid-stimulating hormone (tsh) beta subunit mrna pituitary levels were examined in a teleost, the european silver eel. northern blot analysis showed that the number and length of mrnas encoding tsh beta varied among individuals, a variability apparently not related to thyroidal status. when several bands were present, their intensities were summed for quantitative analysis. increasing circulating thyroid hormones (ths ...19979356215
attempts to analyse anguillicola crassus infection and the humoral host response in eels (anguilla anguilla) of lake balaton, hungary.since the introduction of anguillicola crassus into europe, anguillicolosis has been a considerable problem in several countries. from 1991, periodical eel mortality occurred in lake balaton, hungary. however, eels with a worm burden of 20 to 50 parasites did not show severe swimbladder lesions, which observation cast doubts on the primary aetiological role of the parasite in the eel kill. in order to study the pathology of the infection, from the spring of 1996 until october of the same year, 5 ...19979557321
long-term and seasonal changes in composition and richness of intestinal helminth communities in eels anguilla anguilla of an isolated english river.the need for more long-term studies on helminth communities was addressed by examining changes in composition and diversity of the intestinal helminth component and infracommunities in eels of a small isolated river over 12 years. examination of samples over one summer season indicated that single samples were representative of community richness in that year. in 1985 the community was species poor (1 species only) and with zero diversity, but by 1996 it comprised six species and all parameters ...19979437839
early emergence of three dopamine d1 receptor subtypes in vertebrates. molecular phylogenetic, pharmacological, and functional criteria defining d1a, d1b, and d1c receptors in european eel anguilla anguilla.the existence of dopamine d1c and d1d receptors in xenopus and chicken, respectively, challenged the established duality (d1a and d1b) of the dopamine d1 receptor class in vertebrates. to ascertain the molecular diversity of this gene family in early diverging vertebrates, we isolated four receptor-encoding sequences from the european eel anguilla anguilla. molecular phylogeny assigned two receptor sequences (d1a1 and d1a2) to the d1a subtype, and a third receptor to the d1b subtype. additional ...19979006917
four polymorphic microsatellite markers in the european eel anguilla anguilla (l.). 19979124719
composition and structure of helminth communities in eels anguilla anguilla from italian coastal lagoons.the composition and diversity of total and intestinal helminth component and infracommunities were determined in eels anguilla anguilla from four shallow coastal lagoons near rome. the lagoons differed principally in respect of their salinity. only lake burano still received an input of freshwater, and both freshwater and marine helminth species were found in eels. in the other three lagoons all helminth species were marine or euryhaline and communities were dominated by digeneans. at component ...19979166436
isolation of six putative p-type atpase beta subunit pcr fragments from the brain of the european eel (anguilla anguilla). 19979432906
glutathione-s-transferase is an important antigen in the eel nematodeanguillicola crassusdifferent organs and secretions/excretions of the swimbladder parasite, anguillicola crassus (nematoda), were tested for the presence of antigens to the humoral immune response previously detected in the european eel, anguilla anguilla. proteins from different fractions of anguillicola crassus were separated using sds-page (sodium-dodecyl-sulphate polyacrylamide-gel electrophoresis) under reducing conditions and electroblotted onto nitrocellulose membranes. infected eels showed a specific antibo ...19979443950
polychlorinated biphenyls and organochlorine pesticides in eels (anguilla anguilla l.) from welsh rivers.organochlorine concentrations in the muscle tissues of eels at 41 freshwater sites across wales were surveyed in 1993. positive but weak relationships were detected between eel age or length and the concentration of some organochlorines. isomers of hch were below detection at most sites. dieldrin was widespread at 10-100 microg kt(-1) wet weight, despite its ban in sheep dip in 1989. total ddt residue concentrations averaged 73 microg kg(-1). total pcb burdens expressed as arochlor 1260, were > ...199715093481
residue study of mebendazole and its metabolites hydroxy-mebendazole and amino-mebendazole in eel (anguilla anguilla) after bath treatment.mebendazole (mbz) is extensively used in eel culture for treatment of pseudodactylogyrus spp. infections. this use may lead to residues of mbz in eel tissues. consequently, the residue profile of mbz in eel after treatment with the drug is of special concern. therefore, a residue study was performed in european eels (anguilla anguilla), bath-treated with mbz at a dose of 1 mg/liter for 24 hr and kept at a water temperature of 25 degrees c. liver, kidney, fat, skin, and muscle tissues samples wer ...19979172949
angiotensin ii receptors in the gill of sea water- and freshwater-adapted eel.immunocytochemistry of paraffin-embedded and cryostat sections of eel (anguilla anguilla) gill showed that angiotensin ii receptors (ang ii-r) were present in chloride cells, uniformly distributed in the cytoplasm and on surface membranes. computerised image analysis of these preparations showed that gills from sea water (sw)-adapted animals had a significantly (3-fold) higher ang ii-r concentration compared with freshwater (fw)-adapted eel gills. isoelectric focusing gel electrophoresis reveale ...19979061608
sublethal effects of an organophosphate insecticide on the european eel, anguilla anguilla.the present communication deals with the effects of fenitrothion (0.02 mg/liter) on the energy metabolism of the european eel, anguilla anguilla, and its recovery from intoxication. various parameters such as glycogen, lactate, proteins, and glucose levels were measured in different eel tissues after 2, 8, 12, 24, 32, 48, 56, 72, and 96 hr of fenitrothion exposure. subsequently, the fish were allowed recovery periods of 8, 12, 24, 48, 72, 96, 144, and 192 hr in clean water, and the same paramete ...19979056401
histopathological changes induced by environmental stress in common carp, japanese coloured carp, european eel, and african catfish.histopathological changes caused by stress during catching and transport were examined in four fish species, the common carp (cyprinus carpio), the japanese coloured carp (koi, the coloured variant of the asian carp, cyprinus carpio haematopterus), the european eel (anguilla anguilla), and the african catfish (clarias gariepinus). in all species, the goblet cells of the gills and skin were decreased in number and slight detachment of the epithelium of the secondary gill lamellae was seen. loss o ...19979270124
changes in the electrophysiological parameters of the posterior intestine of anguilla anguilla (pisces) induced by oxytocin, urotensin ii and aldosterone.in view of the importance of the intestine in the osmoregulation of freshwater fishes, we determined the effects of oxytocin, urotensin ii (uii), and aldosterone added to the serosal side of the isolated posterior intestine of the freshwater-adapted teleost anguilla anguilla on electrophysiological parameters. oxytocin decreased the short-circuit current (scc) and transepithelial potential difference (tpd) at concentrations of 1 and 10 mu/ml (to 50% and 42% of control values, respectively), but ...19979222401
h(+)-glycyl-l-proline cotransport in brush-border membrane vesicles of eel (anguilla anguilla) intestine.a plasma membrane h(+)-glycyl-l-proline (gly-l-pro) cotransport mechanism has been identified in isolated eel intestinal brush-border membrane vesicles (bbmv) by both measuring radiolabeled gly-l-pro uptake and monitoring gly-l-pro-dependent h+ influx with the ph-sensitive dye acridine orange. the application of an inside negative membrane potential resulted in increasing gly-l-pro uptake, as well as the application of inwardly directed h+ gradient (although only when an inside negative membrane ...19979039012
induction of erod activity and genotoxic effects by polycyclic aromatic hydrocarbons and resin acids on the juvenile eel (anguilla anguilla l.).the purpose of this research is the study of total ethoxyresorufin o-deethylase (erod) activity and erythrocytic nuclear abnormalities (na) induction by polycyclic aromatic hydrocarbons (pahs) and resin acids (ras) on the juvenile eel anguilla anguilla l. juvenile eels were exposed, during 3 days, to the same molar concentration range (0, 0.1, 0.3, 0.9, and 2.7 microm) of beta-naphthoflavone (bnf) and benzo[a]pyrene (bp)--pahs--and abietic acid (aa) and dehydroabietic acid (dhaa)--ras--and one a ...19979469877
response and recovery of brain acetylcholinesterase activity in the european eel, anguilla anguilla, exposed to fenitrothion.european eel (anguilla anguilla) were exposed to sublethal fenitrothion concentrations in a continuous flow-through system for 4 days. brain acetylcholinesterase (ache) activity was evaluated after 2, 8, 12, 24, 32, 48, 56, 72, and 96 h pesticide exposure. results indicated that ache activity in eel brains decreased as the concentration of fenitrothion increased. the pesticide induced significant inhibitory effects on the ache activity of a. anguilla, ranging from > 40% inhibition at a sublethal ...19979469870
inhibition of gill na+,k+-atpase activity in the eel, anguilla anguilla, by fenitrothion.european eels (anguilla anguilla) were exposed to sublethal fenitrothion concentrations (0.02 and 0.04 mg/liter) in a continuous flow-through system for 4 days. gill mg2+- and na+,k+-atpase activities were evaluated after 2, 8, 12, 24, 32, 48, 56, 72, and 96 h of pesticide exposure. results indicated that atpase activity in gill tissue decreased as concentration of fenitrothion increased. pesticide induced significant inhibitory effects on the na+, k+-atpase activity of a. anguilla, ranging from ...19979417854
effects of vagal stimulation on swimbladder blood flow in the european eel anguilla anguilla.the influence of the vagus nerve on swimbladder blood flow in the european eel (anguilla anguilla) was characterized by recording the changes in blood flow rate and blood pressure following stimulation of the vagus nerve. after electrical stimulation, blood flow in the swimbladder artery increased from 0.9 ml min-1 to 2.1 ml min-1. video recordings of small vessels on the caudal side of the rete mirabile revealed an increase in erythrocyte velocity combined with a small vasodilation. this effect ...19979364021
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