Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| [technical guidelines for the prevention and treatment of chikungunya fever]. | chikungunya fever is an emerging disease caused by an alphavirus belonging to the togaviridae family, transmitted by the bite of aedes genus species: aedesaegypti and aedesalbopictus. in 2013, paho/who received confirmation of the first cases of indigenous transmission of chikungunya in the americas. this disease may be acute, subacute and chronic, affecting all age groups. following an incubation period from three to seven days, the patient usually begins with a high fever (greater than 39 °c), ... | 2017 | 25680650 |
| entomological investigations during early stages of a chikungunya outbreak in the united states virgin islands, 2014. | during the 2014 chikungunya (chik) outbreak in the caribbean, we performed entomological surveys on 3 united states virgin islands (usvi): st. croix, st. thomas, and st. john. we aimed to evaluate the potential for chikungunya virus (chikv) transmission in the usvi. the surveys took place between june 19, 2014, and june 29, 2014, during the dry season in usvi. a total of 1,929 adult mosquitoes belonging to 4 species- culex quinquefasciatus (68.4%), aedes aegypti (29.7%), ae. mediovittatus (1.3%) ... | 2017 | 28388320 |
| potential of aedes aegypti and aedes albopictus populations in the central african republic to transmit enzootic chikungunya virus strains. | major chikungunya outbreaks have affected several central african countries during the past decade. the chikungunya virus (chikv) was isolated from humans and sylvan mosquitoes in the central african republic (car) during the 1970 and 1980s but has not been found recently, despite the presence of aedes albopictus since 2010. the risk of a massive chikungunya epidemic is therefore potentially high, as the human populations are immunologically naïve and because of the presence of the mosquito vect ... | 2017 | 28347325 |
| computer-aided structure based drug design approaches for the discovery of new anti-chikv agents. | chikungunya is a viral infection caused by chikungunya virus (chikv), an arbovirus transmitted through mosquito (aedes aegypti and aedes albopictus) bite. the virus from sylvatic cycle in africa mutated to new vector adaptation and became one of the major emerging and re-emerging viral infections in the past decade, affecting more than 40 countries. efforts are being made by many researches to develop means to prevent and control the infection through vaccines and vector control strategy. on the ... | 2017 | 28294048 |
| chikungunya infection: a global public health menace. | chikungunya virus (chikv) has been involved in epidemics in african and asian subcontinents and, of late, has transcended to affect the americas. aedes aegypti and aedes albopictus are the major vectors for chikv infection, which results in dissemination of virus to various vital organs. entry of virus into these tissues causes infiltration of innate immune cells, monocytes, macrophages, neutrophils, natural killer cells, and adaptive immune cells. macrophages bearing the replicating virus, in t ... | 2017 | 28233156 |
| aedes-borne virus-mosquito interactions: mass spectrometry strategies and findings. | aedes-borne viruses are responsible for high-impact neglected tropical diseases and unpredictable outbreaks such as the ongoing zika epidemics. aedes mosquitoes spread different arboviruses such as dengue virus (denv), chikungunya virus (chikv), and zika virus, among others, and are responsible for the continuous emergence and reemergence of these pathogens. these viruses have complex transmission cycles that include two hosts, namely the aedes mosquito as a vector and susceptible vertebrate hos ... | 2017 | 28192064 |
| environmental and social change drive the explosive emergence of zika virus in the americas. | since zika virus (zikv) was detected in brazil in 2015, it has spread explosively across the americas and has been linked to increased incidence of microcephaly and guillain-barré syndrome (gbs). in one year, it has infected over 500,000 people (suspected and confirmed cases) in 40 countries and territories in the americas. along with recent epidemics of dengue (denv) and chikungunya virus (chikv), which are also transmitted by aedes aegypti and ae. albopictus mosquitoes, the emergence of zikv s ... | 2017 | 28182667 |
| severe chikungunya infection in northern mozambique: a case report. | although chikungunya virus has rapidly expanded to several countries in sub-saharan africa, little attention has been paid to its control and management. until recently, chikungunya has been regarded as a benign and self-limiting disease. in this report we describe the first case of severe chikungunya disease in an adult patient in pemba, mozambique. | 2017 | 28179029 |
| transcriptome analysis of aedes aegypti in response to mono-infections and co-infections of dengue virus-2 and chikungunya virus. | chikungunya virus (chikv) and dengue virus (denv) spread via the bite of infected aedes mosquitoes. both these viruses exist as co-infections in the host as well as the vector and are known to exploit their cellular machinery for their replication. while there are studies reporting the changes in aedes transcriptome when infected with denv and chikv individually, the effect both these viruses have on the mosquitoes when present as co-infections is not clearly understood. in the present study, we ... | 2017 | 28161634 |
| chikungunya virus: an update on the biology and pathogenesis of this emerging pathogen. | re-emergence of chikungunya virus, a mosquito-transmitted pathogen, is of serious public health concern. in the past 15 years, after decades of infrequent, sporadic outbreaks, the virus has caused major epidemic outbreaks in africa, asia, the indian ocean, and more recently the caribbean and the americas. chikungunya virus is mainly transmitted by aedes aegypti mosquitoes in tropical and subtropical regions, but the potential exists for further spread because of genetic adaptation of the virus t ... | 2017 | 28159534 |
| engineered aedes aegypti jak/stat pathway-mediated immunity to dengue virus. | we have developed genetically modified ae. aegypti mosquitoes that activate the conserved antiviral jak/stat pathway in the fat body tissue, by overexpressing either the receptor dome or the janus kinase hop by the blood feeding-induced vitellogenin (vg) promoter. transgene expression inhibits infection with several dengue virus (denv) serotypes in the midgut as well as systemically and in the salivary glands. the impact of the transgenes dome and hop on mosquito longevity was minimal, but it re ... | 2017 | 28081143 |
| identification and initial characterization of matrix metalloproteinases in the yellow fever mosquito, aedes aegypti. | aedes aegypti is a major vector for arboviruses such as dengue, chikungunya and zika viruses. during acquisition of a viremic bloodmeal, an arbovirus infects mosquito midgut cells before disseminating to secondary tissues, including the salivary glands. once virus is released into the salivary ducts it can be transmitted to another vertebrate host. the midgut is surrounded by a basal lamina (bl) in the extracellular matrix, consisting of a proteinaceous mesh composed of collagen iv and laminin. ... | 2017 | 28054419 |
| molecular identification of aedes aegypti mosquitoes from pilani region of rajasthan, india. | aedes aegypti is the most important vector of dengue virus infection in humans worldwide. accurate identification and colonization are the essential requirements to understand vector biology as well as its diseases transmission potential. in this study, we have used molecular approaches for the identification of ae. aegypti mosquitoes that were collected from the pilani region of rajasthan, india methods: field collected mosquito larvae were colonized under laboratory conditions. conserved genet ... | 2017 | 27353585 |
| an analysis of the potential impact of climate change on dengue transmission in the southeastern united states. | dengue fever, caused by a mosquito-transmitted virus, is an increasing health concern in the americas. meteorological variables such as temperature and precipitation can affect disease distribution and abundance through biophysical impacts on the vector and on the virus. such tightly coupled links may facilitate further spread of dengue fever under a changing climate. in the southeastern united states, the dengue vector is widely established and exists on the current fringe of dengue transmissio ... | 2017 | 27713106 |
| the dna chaperone hmgb1 potentiates the transcriptional activity of rel1a in the mosquito aedes aegypti. | high mobility group protein 1 (hmgb1) is a non-histone, chromatin-associated nuclear protein that functions in regulating eukaryotic gene expression. we investigated the influence and mechanism of action of aedes aegypti hmgb1 (aahmgb1) on mosquito rel1a-mediated transcription from target gene promoters. the dna-binding domain (rhd) of aarel1a was bacterially expressed and purified, and aahmgb1 dramatically enhanced rhd binding to consensus nf-kb/rel dna response elements. luciferase reporter an ... | 2017 | 27867076 |
| argonaute 2 suppresses japanese encephalitis virus infection in aedes aegypti. | there are three main innate immune mechanisms against viruses in mosquitoes. infection with the flavivirus dengue virus is controlled by rna interference (rnai) and the jak-stat and toll signaling pathways. this study showed that another flavivirus, japanese encephalitis virus (jev), did not invade the salivary glands of aedes aegypti and that this may be a result of the innate immune resistance to the virus. argonaute 2 (ago2) plays a critical role in the rnai pathway. to understand the mechani ... | 2017 | 27169949 |
| magnetic nanoparticles are highly toxic to chloroquine-resistant plasmodium falciparum, dengue virus (den-2), and their mosquito vectors. | a main challenge in parasitology is the development of reliable tools to prevent or treat mosquito-borne diseases. we investigated the toxicity of magnetic nanoparticles (mnp) produced by magnetospirillum gryphiswaldense (strain msr-1) on chloroquine-resistant (cq-r) and sensitive (cq-s) plasmodium falciparum, dengue virus (den-2), and two of their main vectors, anopheles stephensi and aedes aegypti, respectively. mnp were studied by fourier-transform infrared spectroscopy and transmission elect ... | 2017 | 27815736 |
| predicting arboviral disease emergence using bayesian networks: a case study of dengue virus in western australia. | a bayesian belief network (bbn) for assessing the potential risk of dengue virus emergence and distribution in western australia (wa) is presented and used to identify possible hotspots of dengue outbreaks in summer and winter. the model assesses the probabilities of two kinds of events which must take place before an outbreak can occur: (1) introduction of the virus and mosquito vectors to places where human population densities are high; and (2) vector population growth rates as influenced by ... | 2017 | 27620510 |
| a large scale biorational approach using bacillus thuringiensis israeliensis (strain am65-52) for managing aedes aegypti populations to prevent dengue, chikungunya and zika transmission. | aedes aegypti is a container-inhabiting mosquito and a vector of dengue, chikungunya, and zika viruses. in 2009 several cases of autochthonous dengue transmission were reported in key west, florida, usa prompting a comprehensive response to control a. aegypti. in key west, larvae of this mosquito develop in containers around human habitations which can be numerous and labor intensive to find and treat. aerial applications of larvicide covering large areas in a short time can be an efficient and ... | 2017 | 28199323 |
| levels of insecticide resistance to deltamethrin, malathion, and temephos, and associated mechanisms in aedes aegypti mosquitoes from the guadeloupe and saint martin islands (french west indies). | in the guadeloupe and saint martin islands, aedes aegypti mosquitoes are the only recognized vectors of dengue, chikungunya, and zika viruses. for around 40 years, malathion was used as a mosquito adulticide and temephos as a larvicide. since the european union banned the use of these two insecticide molecules in the first decade of the 21st century, deltamethrin and bacillus thuringiensis var. israelensis are the remaining adulticide and larvicide, respectively, used in guadeloupe. in order to ... | 2017 | 28187780 |
| response of three cyprinid fish species to the scavenger deterrent factor produced by the mutualistic bacteria associated with entomopathogenic nematodes. | the symbiotic bacteria, photorhabdus and xenorhabdus associated with entomopathogenic nematodes (epns) in the genera heterorhabditis and steinernema, respectively, produce a compound(s) called the scavenging deterrent factor (sdf). sdf deters a number of terrestrial insect scavengers and predators and one bird species from feeding on host insects killed by the nematode-bacterium complex but has not been tested against aquatic vertebrates. moreover, the heterorhabditis-photorhabdus association is ... | 2017 | 27908637 |
| [clinical and epidemiological profile of chikungunya infection in hospital patients in 2015 in mérida, mexicoperfil clínico-epidemiológico da infecção por chikungunya em casos hospitalares atendidos em 2015 em mérida, méxico]. | describe the epidemiological profile and analyze clinical manifestations by age group in chikungunya patients treated in 2015 in a mérida, mexico general hospital. | 2017 | 31391823 |
| erratum: efficacy of some wearable devices compared with spray-on insect repellents for the yellow fever mosquito, aedes aegypti (l.) (diptera: culicidae). | 2017 | 28365772 | |
| tropical viruses approaching europe. | during the last years, headlines about the advance of tropical infectious diseases, which are transmitted by mosquitos, become more frequent. some of these mosquitos are classified as invasive species in europe. the world health organization (who) and the european center for disease prevention and control (ecdc) are enhancing the observation of the geographical spread of these species. especially the asian tiger mosquito and the yellow fever mosquito are in focus. in germany the vector competenc ... | 2017 | 29952164 |
| tropical viruses approaching europe. | during the last years, headlines about the advance of tropical infectious diseases, which are transmitted by mosquitos, become more frequent. some of these mosquitos are classified as invasive species in europe. the world health organization (who) and the european center for disease prevention and control (ecdc) are enhancing the observation of the geographical spread of these species. especially the asian tiger mosquito and the yellow fever mosquito are in focus. in germany the vector competenc ... | 2017 | 29952163 |
| environmental factors can influence dengue reported cases. | global climate changes directly affect the natural environment and contribute to an increase in the transmission of diseases by vectors. among these diseases, dengue is at the top of the list. the aim of our study was to understand the consequences of temporal variability of air temperature in the occurrence of dengue in an area comprising seven municipalities of the greater são paulo. | 2017 | 29451659 |
| [(assessment of risk for arbovirus infections in russia)]. | the sharply increased incidence and geographical expansion of arbovirus diseases in recent years suggest that some russian areas are vulnerable to vector-borne diseases and that it is important to elaborate control programs to ensure bio- logical safety in our country. moreover, the main vectors of the pathogens of arbovirus infections (zika fever, dengue fever, chikungunya fevers), such as aedes aegypti and ae.albopictus, have been registered on the black sea coast of the caucasus. the programs ... | 2017 | 30721608 |
| larvicidal and pupicidal activities of crude and fractionated extracts of acacia pennata (l.) willd. subsp insuavis shoot tips against aedes aegypti (l.) (diptera: culicidae). | acacia pennata subsp insuavis, or cha-om in thai, is a common vegetablefound in thailand. it has been used as a medicinal herb for a long time. from theliterature, antinociceptive, anti-inflammatory, antimicrobial, and anti-helminthicactivities were reported. in this study, we investigated two new actions of thisplant: larvicide and pupicide. the crude ethanolic and fractionated extracts of a.pennata shoot tips were tested against aquatic stages of the dengue virus vector,aedes aegypti mosquito. ... | 2017 | 29644817 |
| fly ash-based water dispersible powder formulation of bacillus thuringiensis var. israelensis: development & laboratory evaluation against mosquito immatures. | bacillus thuringiensis var. israelensis (bti) formulations are presently being used for insect control. in this study, a water dispersible powder (wdp) formulation using fly ash (fa) as a carrier material was developed and studied for its activity against the larval stages of major mosquito vector species. | 2017 | 29664029 |
| infectivity of chordodes nobilii larvae (gordiida: nematomorpha). | the gordiids are freshwater representatives of the parasite phylum nematomorpha that function asa link between aquatic and terrestrial ecosystems. in recent years, different ecotoxicologic studies have beenmade with the south-american gordiid species, chordodes nobilii, that have demonstrated the capacity of thisgroup to act as a bioindicator of contamination. despite the gordiida’s ecologic relevance, further studies arestill needed to elucidate different aspects of the biology of the class, an ... | 2017 | 29465953 |
| ultrasound is not useful as a screening tool for dengue fever. | dengue fever is a tropical disease that is transmitted by female aedes aegypti mosquitos. early diagnosis is necessary to reduce the mortality and morbidity associated with the disease. a combination of clinical, laboratory, and sonography findings can be potentially helpful in making an early diagnosis of dengue fever. there is paucity of literature on the use of ultrasound for dengue fever screening; hence, the primary objective of the study was to evaluate the utility of ultrasound as a scree ... | 2017 | 29657635 |
| estimating the size of aedes aegypti populations from dengue incidence data: implications for the risk of yellow fever outbreaks. | in this paper we present a model to estimate the density of aedes mosquitoes in a community affected by dengue. the method consists in fitting a continuous function to the incidence of dengue infections, from which the density of infected mosquitoes is derived straightforwardly. further derivations allow the calculation of the latent and susceptible mosquitoes' densities, the sum of the three equals the total mosquitoes' density. the method is illustrated with the case of the risk of urban yello ... | 2017 | 30137722 |
| evaluation of phytosynthesised silver nanoparticles from leaf extracts of leucas aspera and hyptis suaveolens and their larvicidal activity against malaria, dengue and filariasis vectors. | the present study deals with the green synthesis of silver nanoparticle from the aqueous leaf extracts of leucas aspera and hyptis suaveolens as reducing agent and to investigate the larvicidal activity of synthesized silver nanoparticles. the synthesized silver nanoparticles were characterized by ultraviolet and visible absorption spectroscopy (uv), fourier transform-infrared spectroscopy (ft-ir), x-ray spectroscopy (xrd), field emission scanning electron microscope (fesem) and high-resonance t ... | 2017 | 29774292 |
| biological control of anopheles darlingi, aedes aegypti and culex quinquefasciatus larvae using shrimps. | mosquitoes can act as vectors of important diseases such as malaria, dengue, zika virus, yellow fever, chikungunya and mayaro fever, in addition to filariasis. the use of insecticides, larvicides, bed nets and repellents, besides the use of drugs as chemoprevention and the treatment of the sick are currently the pillars of the control of these vectors. we studied the biological control against of anopheles darlingi, aedes aegypti and culex quinquefasciatus larvae using shrimps of the species m. ... | 2017 | 29774286 |
| the influence of urban heat islands and socioeconomic factors on the spatial distribution of aedes aegypti larval habitats. | we addressed the potential associations among the temporal and spatial distribution of larval habitats of aedes (stegomyia) aegypti, the presence of urban heat islands and socioeconomic factors. data on larval habitats were collected in santa bárbara d'oeste, são paulo, brazil, from 2004 to 2006, and spatial and temporal variations were analysed using a wavelet-based approach. we quantified urban heat islands by calculating surface temperatures using the results of wavelet analyses and grey leve ... | 2018 | 29772891 |
| urban environmental clustering to assess the spatial dynamics of aedes aegypti breeding sites. | the identification of aedes aegypti breeding hotspots in urban areas is crucial for the rational design of control strategies against this disease vector. remote sensing and geographic information systems offer valuable tools for mapping habitat suitability of a given area. however, predicting species occurrences by means of probability distribution maps based on transversal entomological surveys has limited utility for local authorities. the aim of the present study was to carefully examine the ... | 2018 | 29772886 |
| encapsidated host factors in alphavirus particles influence midgut infection of aedes aegypti. | transmission of mosquito-borne viruses requires the efficient infection of both a permissive vertebrate host and a competent mosquito vector. the infectivity of sindbis virus (sinv), the type species of the alphavirus genus, is influenced by both the original and new host cell. we have shown that infection of vertebrate cells by sinv, chikungunya virus (chikv), and ross river virus (rrv) produces two subpopulations of virus particles separable based on density. in contrast, a single population o ... | 2018 | 29772674 |
| tracking aedes aegypti (diptera: culicidae) larval behavior across development: effects of temperature and nutrients on individuals' foraging behavior. | immature mosquitoes alter their foraging behavior in response to variation in nutrients, predators, and temperature, with consequences on the adult stage where pathogens are transmitted. these patterns of behavior have not been described with respect to both developmental stage and environmental variation, nor has behavior been examined within an individual across instars. we hypothesized that individual larvae have distinct behavioral syndromes, and predict that the rank of foraging activity in ... | 2018 | 29771372 |
| niche conservatism of aedes albopictus and aedes aegypti - two mosquito species with different invasion histories. | biological invasions have been associated with niche changes; however, their occurrence is still debated. we assess whether climatic niches between native and non-native ranges have changed during the invasion process using two globally spread mosquitoes as model species, aedes albopictus and aedes aegypti. considering the different time spans since their invasions (>300 vs. 30-40 years), niche changes were expected to be more likely for ae. aegypti than for ae. albopictus. we used temperature a ... | 2018 | 29769652 |
| ornamental bromeliads of miami-dade county, florida are important breeding sites for aedes aegypti (diptera: culicidae). | a major public health concern is the emergence and geographical spread of vector-borne diseases such as zika and yellow fever. ornamental bromeliads retaining water in their leaf axils represent potential breeding sites for mosquitoes. as the role of ornamental bromeliads in breeding aedes aegypti in miami-dade county, florida is unknown, we hypothesize that ornamental bromeliads are important breeding sites for ae. aegypti. our objective was to survey bromeliads in areas with high densities of ... | 2018 | 29769105 |
| efficacy of the main repellents available in the brazilian market against aedes aegypti bites under concentrations applied to pediatric populations. | 2018 | 29768569 | |
| ns1 codon usage adaptation to humans in pandemic zika virus. | zika virus (zikv) was recognised as a zoonotic pathogen in africa and southeastern asia. human infections were infrequently reported until 2007, when the first known epidemic occurred in micronesia. after 2013, the asian lineage of zikv spread along the pacific islands and americas, causing severe outbreaks with millions of human infections. the recent human infections of zikv were also associated with severe complications, such as an increase in cases of guillain-barre syndrome and the emergenc ... | 2018 | 29768530 |
| pyrethroid insecticides maintain repellent effect on knock-down resistant populations of aedes aegypti mosquitoes. | pyrethroid-treated clothing is commonly worn for protection against mosquitoes; pyrethroids are both insecticides and repellents. pyrethroid resistance has become increasingly common in aedes aegypti, the vector of dengue, zika, and other arboviruses, but it is not clear whether resistance is associated with reductions in repellency. in order to determine whether long-lasting permethrin impregnated (llpi) clothing is protective, we used aedes aegypti from new orleans, la (pyrethroid-sensitive) a ... | 2018 | 29763445 |
| effects of light and temperature on daily activity and clock gene expression in two mosquito disease vectors. | most organisms feature an endogenous circadian clock capable of synchronization with their environment. the most well-known synchronizing agents are light and temperature. the circadian clock of mosquitoes, vectors of many pathogens, drives important behaviors related to vectoral capacity, including oviposition, host seeking, and hematophagy. main clock gene expression, as well as locomotor activity patterns, has been identified in aedes aegypti and culex quinquefasciatus under artificial light- ... | 2018 | 29761749 |
| threats of zika virus transmission for asia and its hindu-kush himalayan region. | asia and its hindu kush himalayan (hkh) region is particularly vulnerable to environmental change, especially climate and land use changes further influenced by rapid population growth, high level of poverty and unsustainable development. asia has been a hotspot of dengue fever and chikungunya mainly due to its dense human population, unplanned urbanization and poverty. in an urban cycle, dengue virus (denv) and chikungunya virus (chikv) are transmitted by aedes aegypti and ae. albopictus mosqui ... | 2018 | 29759076 |
| aedes aegypti(linnaeus) larvae from dengue outbreak areas in selangor showing resistance to pyrethroids but susceptible to organophosphates. | the resistance status of selangor aedes aegypti (linnaeus) larvae against four major groups of insecticides (i.e., organochlorines, carbamates, organophosphates and pyrethroids) was investigated. aedes aegypti were susceptible against temephos (organophosphate), although resistance (rr50 = 0.21-2.64) may be developing. the insecticides susceptibility status of ae. aegypti larvae were found heterogeneous among the different study sites. results showed that ae. aegypti larvae from klang, sabak ber ... | 2018 | 29758171 |
| a review of the ongoing research on zika virus treatment. | the zika fever is an arboviral disease resulting from the infection with zika virus (zikv). the virus is transmitted to humans by the bite of aedes mosquitos, mainly aedes aegypti and aedes albopictus. zikv has been detected for decades in african and asian regions and, since 2007, has spread to other continents; among them, infections are most reported in the americas. this can be explained by the presence of vectors in highly populated and tropical regions where people are susceptible to conta ... | 2018 | 29758005 |
| two novel mutations in the voltage-gated sodium channel associated with knockdown resistance (kdr) in the dengue vector aedes aegypti in vietnam. | 2018 | 29757514 | |
| diel patterns of aedes aegypti (diptera: culicidae) after resurgence in st. augustine, florida as collected by a mechanical rotator trap. | 2018 | 29757509 | |
| mosquito traps for urban surveillance: collection efficacy and potential for use by citizen scientists. | mosquito-borne diseases are a pervasive public health problem on a global scale, and effective management of them requires well-designed surveillance programs for both vectors and pathogens. mosquito traps are a common component of such programs, and their reach can be expanded by engaging citizen scientists. in this study in a southern australian city, we compared the mosquito collection efficacy of two types of traps and assessed their suitability for use in citizen science programs. bg sentin ... | 2018 | 29757507 |
| controversies over the scientific name of the principal mosquito vector of yellow fever virus - expediency versus validity. | the history of the scientific name of the yellow fever mosquito, the vector of yellow fever virus, ranges from 1757 to the early twenty-first century. in his 1757 work iter palaestinum, frederic hasselquist gave the name culex aegypti to a mosquito species responsible for fierce attacks on humans in egypt. that name was never later ascribed to hasselquist as author, but to linnaeus, although the name never appeared in any of linnaeus' publications. in cuba, at the end of the nineteenth century, ... | 2018 | 29757506 |
| effects of scarcity and excess of larval food on life history traits of aedes aegypti (diptera: culicidae). | few studies have assessed the effects of food scarcity or excess on the life history traits of aedes aegypti (l.) (diptera: culicidae) independently from larval density. we assessed immature survival, development time, and adult size in relation to food availability. we reared cohorts of 30 ae. aegypti larvae from newly hatched to adult emergence with different food availability. food conditions were kept constant by transferring larvae each day to a new food solution. immature development was c ... | 2018 | 29757503 |
| de novo dna synthesis in aedes aegypti midgut cells as a complementary strategy to limit dengue viral replication. | aedes aegypti is the main vector of dengue virus, carrying the virus during the whole mosquito life post-infection. few mosquito fitness costs have been associated to the virus infection, thereby allowing for a swift dissemination. in order to diminish the mosquito population, public health agency use persistent chemicals with environmental impact for disease control. most countries barely use biological controls, if at all. with the purpose of developing novel dengue control strategies, a detai ... | 2018 | 29755433 |
| sargassum wightii-synthesized zno nanoparticles - from antibacterial and insecticidal activity to immunostimulatory effects on the green tiger shrimp penaeus semisulcatus. | the green synthesis of metal nanoparticles using phytochemical from marine seaweeds is a fast-growing research field in nanotechnology. here, the biosynthesis of zinc oxide nanoparticles was achieved using the hot water extract of sargassum wightii. the hot water extract prepared from s. wightii (h sw) and zno nps were studied by uv-visible and ftir spectroscopy, sem and xrd. then, both products were evaluated for antibiofilm activity towards aquatic pathogens. the nanoparticles' immunostimulati ... | 2018 | 29754050 |
| dynamics of dengue disease with human and vector mobility. | dengue is a vector borne disease transmitted to humans by aedes aegypti mosquitoes carrying virus of different serotypes. dengue exhibits complex spatial and temporal dynamics, influenced by various biological, human and environmental factors. in this work, we study the dengue spread for a single serotype (denv-1) including statistical models of human mobility with exponential step length distribution, by using reaction-diffusion equations and stochastic cellular automata (sca) approach. we anal ... | 2018 | 29751893 |
| a wolbachia triple-strain infection generates self-incompatibility in aedes albopictus and transmission instability in aedes aegypti. | artificially-introduced transinfections of the intracellular bacterium wolbachia pipientis have the potential to reduce the vectorial capacity of mosquito populations for viruses such as dengue and chikungunya. aedes albopictus has two native strains of wolbachia, but their replacement with the non-native wmel strain blocks transmission of both viruses. the pattern of cytoplasmic incompatiiblity generated by wmel with wild-types is bidirectional. novel-plus-native-strain co-infection is predicte ... | 2018 | 29751814 |
| molecular responses to the zika virus in mosquitoes. | the zika virus (zikv), originally discovered in 1947, did not become a major concern until the virus swept across the pacific and into the americas in the last decade, bringing with it news of neurological complications and birth defects in zikv affected areas. this prompted researchers to dissect the molecular interactions between zikv and the mosquito vector in an attempt to better understand not only the changes that occur upon infection, but to also identify molecules that may potentially en ... | 2018 | 29751526 |
| effect of uv-b irradiation on water-suspended metarhizium anisopliae s.l. (hypocreales: clavicipitaceae) conidia and their larvicidal activity in aedes aegypti (diptera: culicidae). | ultraviolet (uv) radiation is a key limiting factor for biological pest control with entomopathogenic fungi. while little is known about the impact of uv on metarhizium anisopliae metchnikoff (sorokin) (hypocreales: clavicipitaceae) conidia in aquatic mosquito-breeding sites, this study determined the effect of uv-b on the viability and virulence of m. anisopliae sensu lato (s.l.) strain ip 46 in the laboratory against aedes aegypti (l.) (diptera: culicidae) larvae. conidia were treated in cups ... | 2018 | 29750411 |
| experimental zika virus infection in aedes aegypti: susceptibility, transmission & co-infection with dengue & chikungunya viruses. | there are reports about the susceptibility of aedes mosquitoes to zikv from various countries, however, no such information is available from indian sub-continent, although, high level of group cross-reactivity of zikv with other flaviviruses has been reported. during outbreak situations, many cases of dengue (den) and chikungunya (chik) are reported. in such scenario, vector mosquitoes are likely to get co-infection/secondary-infection with one or other virus. the present study was carried out ... | 2018 | 29749366 |
| a new "american" subgroup of african-lineage chikungunya virus detected in and isolated from mosquitoes collected in haiti, 2016. | as part of on-going arboviral surveillance activity in a semi-rural region in haiti, chikungunya virus (chikv)-positive mosquito pools were identified in 2014 (the peak of the caribbean asian-clade epidemic), and again in 2016 by rt-pcr. in 2014, chikv was only identified in aedes aegypti (11 positive pools/124 screened). in contrast, in sampling in 2016, chikv was not identified in ae. aegypti, but, rather, in (a) a female aedes albopictus pool, and (b) a female culex quinquefasciatus pool. gen ... | 2018 | 29746539 |
| seasonal temperature variation influences climate suitability for dengue, chikungunya, and zika transmission. | dengue, chikungunya, and zika virus epidemics transmitted by aedes aegypti mosquitoes have recently (re)emerged and spread throughout the americas, southeast asia, the pacific islands, and elsewhere. understanding how environmental conditions affect epidemic dynamics is critical for predicting and responding to the geographic and seasonal spread of disease. specifically, we lack a mechanistic understanding of how seasonal variation in temperature affects epidemic magnitude and duration. here, we ... | 2018 | 29746468 |
| atypical neurological manifestations of chikungunya fever: two case reports. | chikungunya fever (chik) is an arboviral disease which is transmitted by a mosquito bite (aedes aegypti). fever, polyarthralgia, and maculopapular rash are hallmark clinical manifestations of this disease. these manifestations are self-liming and complete recovery is seen in most of the cases. however, atypical and lethal complications such as myocarditis, hepatitis, guillain-barre syndrome (gbs), and meningoencephalitis have been reported in few cases of chik. we hereby report two cases of chik ... | 2018 | 29743772 |
| complete genome of aedes aegypti anphevirus in the aag2 mosquito cell line. | a novel negative-sense rna virus, aedes aegypti anphevirus, was recently identified in wild aedes aegypti mosquitoes. we show that this virus is also present in the aag2 aedes aegypti cell line and characterize its complete genome and evolutionary history. the aedes aegypti anphevirus genome is estimated to be 12 916 nucleotides in length, contains four genes and has a genome structure similar to that of other anpheviruses. phylogenetically, aedes aegypti anphevirus falls within an unclassified ... | 2018 | 29741476 |
| expression of a synthetic gene for the major cytotoxin (cyt1aa) of bacillus thuringiensis subsp. israelensis in the chloroplast of wild-type chlamydomonas. | chlamydomonas reinhardtii (chlamydomonas) strains that are toxic to mosquito larvae because they express chloroplast transgenes that are based on the mosquitocidal proteins of bacillus thuringiensis subsp. israelensis (bti) could be very useful in mosquito control. chlamydomonas has several advantages for this approach, including genetic controls not generally available with industrial algae. the bti toxin is produced by sporulating bacteria and has been used for mosquito control for >30 years w ... | 2018 | 29738473 |
| crystal structure of a novel kunitz type inhibitor, alocasin with anti-aedes aegypti activity targeting midgut proteases. | the pesticidal properties of many kunitz-type inhibitors have been reported previously; however, the mechanism of action is not well established. in this study, the activity of alocasin against aedes aegypti is demonstrated and the structure-activity relationship of this kunitz-type inhibitor is explained through x-ray structure analyses. | 2018 | 29737039 |
| antibodies against chikungunya in northern mozambique during dengue outbreak, 2014. | an outbreak of dengue and high densities of aedes aegypti were reported in 2014 in northern mozambique, suggesting an increased risk for other arboviruses such as chikungunya virus (chikv) in this region. the aim of this study was to investigate the occurrence of chikv during an outbreak of dengue virus (denv) in pemba city in northern mozambique in 2014. febrile patients (n = 146) seeking medical attention at the pemba provincial hospital between march and april 2014 were enrolled in this study ... | 2018 | 29733254 |
| male contributions during mating increase female survival in the disease vector mosquito aedes aegypti. | aedes aegypti is a vector of medically important viruses including those causing zika, dengue, and chikungunya. during mating, males transfer a number of proteins and other molecules to the female and these components of the male ejaculate are essential in shifting female post-mating behaviors in a number of insect species. because these molecules are highly variable by species, and female post-mating behavior by species is also varied, behavioral assays testing the function of the ejaculate are ... | 2018 | 29729859 |
| improving the delivery and efficiency of fungus-impregnated cloths for control of adult aedes aegypti using a synthetic attractive lure. | entomopathogenic fungi are highly promising agents for controlling aedes aegypti mosquitoes. deploying fungus-impregnated black cloths in pet traps efficiently reduced ae. aegypti female survival rates under intra-domicile conditions. with the aim of further increasing the effectiveness of the traps, the addition of attractive lures to fungus-impregnated traps was evaluated. | 2018 | 29728121 |
| advising a woman with suspected zika virus infection. | the zika virus (zikv) epidemic is spreading: 67 countries are now reporting transmission, and over 2,000 cases of congenital zika syndrome (czs) have been confirmed. the heaviest burden has been borne by those living where poverty, poor infrastructure, and lack of access to health services are common and the penetration of aedes aegypti is high. because most cases are asymptomatic, the most dramatic signs of the disease appear through the czs cases. in spite of the need for disaggregated epidemi ... | 2018 | 29724698 |
| larval density mediates knockdown resistance to pyrethroid insecticides in adult aedes aegypti. | understanding mechanisms driving insecticide resistance in vector populations remains a public health priority. to date, most research has focused on the genetic mechanisms underpinning resistance, yet it is unclear what role environmental drivers may play in shaping phenotypic expression. one of the key environmental drivers of aedes aegypti mosquito population dynamics is resource-driven intraspecific competition at the larval stage. we experimentally investigated the role of density-dependent ... | 2018 | 29724237 |
| contributions from the silent majority dominate dengue virus transmission. | despite estimates that, each year, as many as 300 million dengue virus (denv) infections result in either no perceptible symptoms (asymptomatic) or symptoms that are sufficiently mild to go undetected by surveillance systems (inapparent), it has been assumed that these infections contribute little to onward transmission. however, recent blood-feeding experiments with aedes aegypti mosquitoes showed that people with asymptomatic and pre-symptomatic denv infections are capable of infecting mosquit ... | 2018 | 29723307 |
| wolbachia significantly impacts the vector competence of aedes aegypti for mayaro virus. | wolbachia, an intracellular endosymbiont present in up to 70% of all insect species, has been suggested as a sustainable strategy for the control of arboviruses such as dengue, zika and chikungunya. as mayaro virus outbreaks have also been reported in latin american countries, the objective of this study was to evaluate the vector competence of brazilian field-collected ae. aegypti and the impact of wolbachia (wmel strain) upon this virus. our in vitro studies with aag2 cells showed that mayaro ... | 2018 | 29720714 |
| vector competence of certain culex and aedes mosquitoes for the chittoor virus, the indian variant of the batai virus. | chittoor virus (chitv), a mosquito-borne bunyavirus (orthobunyavirus: bunyaviridae) isolated in india, has been found to be antigenically close to the batai virus (batv), which has a wide distribution across asia, europe, and africa. the latter virus causes influenza-like illness in humans and mild illness in sheep and goats. batv has been involved in genetic reassortment with other bunyaviruses, generating novel genome combinations and causing severe clinical manifestations including hemorrhagi ... | 2018 | 29718685 |
| high throughput discovery of protein variants using proteomics informed by transcriptomics. | proteomics informed by transcriptomics (pit), in which proteomic ms/ms spectra are searched against open reading frames derived from de novo assembled transcripts, can reveal previously unknown translated genomic elements (tges). however, determining which tges are truly novel, which are variants of known proteins, and which are simply artefacts of poor sequence assembly, is challenging. we have designed and implemented an automated solution that classifies putative tges by comparing to referenc ... | 2018 | 29718325 |
| dehydration prompts increased activity and blood feeding by mosquitoes. | current insights into the mosquito dehydration response rely on studies that examine specific responses but ultimately fail to provide an encompassing view of mosquito biology. here, we examined underlying changes in the biology of mosquitoes associated with dehydration. specifically, we show that dehydration increases blood feeding in the northern house mosquito, culex pipiens, which was the result of both higher activity and a greater tendency to land on a host. similar observations were noted ... | 2018 | 29717151 |
| efficacy of larvicides for the control of dengue, zika, and chikungunya vectors in an urban cemetery in southern mexico. | many countries in latin america have recently experienced outbreaks of zika and chikungunya fever, in additional to the usual burden imposed by dengue, all of which are transmitted by aedes aegypti in this region. to identify potential larvicides, we determined the toxicity of eight modern insecticides to a. aegypti larvae from a colony that originated from field-collected insects in southern mexico. the most toxic compounds were pyriproxyfen (which prevented adult emergence) and λ-cyhalothrin, ... | 2018 | 29713901 |
| parallel evolution of vgsc mutations at domains is6, iis6 and iiis6 in pyrethroid resistant aedes aegypti from mexico. | aedes aegypti is the primary urban mosquito vector of viruses causing dengue, zika and chikungunya fevers -for which vaccines and effective pharmaceuticals are still lacking. current strategies to suppress arbovirus outbreaks include removal of larval-breeding sites and insecticide treatment of larval and adult populations. insecticidal control of ae. aegypti is challenging, due to a recent rapid global increase in knockdown-resistance (kdr) to pyrethroid insecticides. widespread, heavy use of p ... | 2018 | 29712956 |
| identification of candidate iron transporters from the zip/znt gene families in the mosquito aedes aegypti. | mosquito-transmitted viral pathogens, such as dengue and zika, afflict tens of thousands of people every year. these viruses are transmitted during the blood-feeding process that is required for mosquito reproduction, the most important vector being aedes aegypti. while vertebrate blood is rich in protein, its high iron content is potentially toxic to mosquitoes. although iron transport and sequestration are essential in the reproduction of vector mosquitoes, we discovered that culicine mosquito ... | 2018 | 29706902 |
| dengue fever in lebanon: first confirmed case since 1945 and review from the region. | lebanon yearly witnesses a high flux of expatriates and workers from dengue virus (denv) endemic regions. multiple cases of dengue fever have been documented at the american university of beirut medical center (aubmc) in travellers to endemic regions. given the presence of the aedes aegypti mosquito in lebanon, introduction of denv to the country is highly likely. we report a case of denv infection in lebanon diagnosed in april 2012 in a patient with no prior travel history. the patient presente ... | 2018 | 31851640 |
| [applications of the crispr/cas9 system in insects]. | the crispr/cas9 (clustered regularly interspaced short palindromic repeat/crispr-associated) system guides cas9 to specific genomic locations by a short rna search string. this technology enables the systematic interrogation of mammalian genome editing, repairing damaged genes, silencing harmful genes and improving quality traits. in recent years, with the introduction of the crispr/cas9 system for easy, fast and efficient genetic modification, it has been possible to conduct meaningful function ... | 2018 | 29704373 |
| structural characterization of bacillus licheniformis dahb1 exopolysaccharide-antimicrobial potential and larvicidal activity on malaria and zika virus mosquito vectors. | microbial polysaccharides produced by marine species play a key role in food and cosmetic industry, as they are nontoxic and biodegradable polymers. this investigation reports the isolation of exopolysaccharide from bacillus licheniformis dahb1 and its biomedical applications. bacillus licheniformis dahb1 exopolysaccharide (bl-eps) was extracted using the ethanol precipitation method and structurally characterized. ftir and 1h-nmr pointed out the presence of various functional groups and primary ... | 2018 | 29704178 |
| host genotype and environment affect the trade-off between horizontal and vertical transmission of the parasite edhazardia aedis. | if a parasite is able to transmit horizontally or vertically, which transmission mode will it choose? we investigated how the growth conditions and the genotype of the mosquito aedes aegypti affect the transmission mode of the parasite edhazardia aedis. | 2018 | 29699504 |
| ammonia excretion in an osmoregulatory syncytium is facilitated by aeamt2, a novel ammonia transporter in aedes aegypti larvae. | the larvae of the mosquito aedes aegypti inhabit ammonia rich septic tanks in tropical regions of the world that make extensive use of these systems, explaining the prevalence of disease during dry seasons. since ammonia (nh3/[formula: see text]) is toxic to animals, an understanding of the physiological mechanisms of ammonia excretion permitting the survival of a. aegypti larvae in high ammonia environments is important. we have characterized a novel ammonia transporter, aeamt2, belonging to th ... | 2018 | 29695971 |
| a chikungunya virus trans-replicase system reveals the importance of delayed nonstructural polyprotein processing for efficient replication complex formation in mosquito cells. | chikungunya virus (chikv) is a medically important alphavirus that is transmitted by aedes aegypti and aedes albopictus mosquitoes. the viral replicase complex consists of four nonstructural proteins (nsps) expressed as a polyprotein precursor and encompasses all enzymatic activities required for viral rna replication. nsps interact with host components of which most are still poorly understood, especially in mosquitos. a chikv trans-replicase system that allows the uncoupling of rna replication ... | 2018 | 29695432 |
| effects of desiccation stress on adult female longevity in aedes aegypti and ae. albopictus (diptera: culicidae): results of a systematic review and pooled survival analysis. | transmission dynamics of mosquito-borne viruses such as dengue, zika and chikungunya are affected by the longevity of the adult female mosquito. environmental conditions influence the survival of adult female aedes mosquitoes, the primary vectors of these viruses. while the association of temperature with aedes mortality has been relatively well-explored, the role of humidity is less established. the current study's goals were to compile knowledge of the influence of humidity on adult survival i ... | 2018 | 29695282 |
| dna barcoding of morphologically characterized mosquitoes belonging to the subfamily culicinae from sri lanka. | vectors of mosquito-borne diseases in sri lanka, except for malaria, belong to the subfamily culicinae, which includes nearly 84% of the mosquito fauna of the country. hence, accurate and precise species identification of culicine mosquitoes is a crucial factor in implementing effective vector control strategies. during the present study, a combined effort using morphology and dna barcoding was made to characterize mosquitoes of the subfamily culicinae for the first time from nine districts of s ... | 2018 | 29695263 |
| characterization of a western pacific zika virus strain in australian aedes aegypti. | zika virus (zikv) is a globally emerging arbovirus responsible for widespread epidemics in the western pacific, the americas, and asia. the virus predominately circulates in urban transmission cycles between aedes aegypti and humans. australia is considered at risk to outbreaks of zikv due to the presence of a. aegypti populations in northern areas of the state of queensland. furthermore, close proximity to epidemic regions has led to almost 50% of imported cases reported since 2012 originating ... | 2018 | 29694294 |
| neurotropism and behavioral changes associated with zika infection in the vector aedes aegypti. | understanding zika virus infection dynamics is essential, as its recent emergence revealed possible devastating neuropathologies in humans, thus causing a major threat to public health worldwide. recent research allowed breakthrough in our understanding of the virus and host pathogenesis; however, little is known on its impact on its main vector, aedes aegypti. here we show how zika virus targets aedes aegypti's neurons and induces changes in its behavior. results are compared to dengue virus, a ... | 2018 | 29691362 |
| dengue virus infection induces chromatin remodeling at locus aael006536 in the midgut of aedes aegypti. | to identify and characterize aedes aegypti's aael006536 gene proximal upstream cis-regulatory sequences activated by dengue virus infection. | 2018 | 29689655 |
| association of dengue fever with aedes spp. abundance and climatological effects. | to analyze the association of dengue fever incidence with aedes mosquito's abundance, and the effect of climatological and geographical variables, in a region in morelos state, mexico. | 2018 | 29689652 |
| the redox-sensing gene nrf2 affects intestinal homeostasis, insecticide resistance, and zika virus susceptibility in the mosquito aedes aegypti. | production and degradation of reactive oxygen species (ros) are extensively regulated to ensure proper cellular responses to various environmental stimuli and stresses. moreover, physiologically generated ros function as secondary messengers that can influence tissue homeostasis. the cap'n'collar transcription factor known as nuclear factor erythroid-derived factor 2 (nrf2) coordinates an evolutionarily conserved transcriptional activation pathway that mediates antioxidant and detoxification res ... | 2018 | 29685890 |
| dispersion and oviposition of aedes albopictus in a brazilian slum: initial evidence of asian tiger mosquito domiciliation in urban environments. | aedes albopictus, originally considered as a secondary vector for arbovirus transmission, especially in areas where this species co-exist with aedes aegypti, has been described in most regions of the world. dispersion and domiciliation of ae. albopictus in a complex of densely urbanized slums in rio de janeiro, southeastern brazil, was evidenced. in this study, we tested the hypotheses that 1) ae. albopictus distribution in urban slums is negatively related to distance from vegetation, and 2) th ... | 2018 | 29684029 |
| entomopathogenic fungal infection leads to temporospatial modulation of the mosquito immune system. | alternative methods of mosquito control are needed to tackle the rising burden of mosquito-borne diseases while minimizing the use of synthetic insecticides, which are threatened by the rapid increase in insecticide resistance in mosquito populations. fungal biopesticides show great promise as potential alternatives because of their ecofriendly nature and ability to infect mosquitoes on contact. here we describe the temporospatial interactions between the mosquito aedes aegypti and several entom ... | 2018 | 29684026 |
| dengue fever and aedes aegypti in indigenous brazilians: seroprevalence, risk factors, knowledge and practices. | dengue remains an important public health problem in brazil. we estimated the associated factors of dengue seroprevalence among native indians of the tremembé ethnic and their knowledge about the aspects related to the presence of mosquitoes of the genus aedes. | 2018 | 29673030 |
| susceptibility status and resistance mechanisms in permethrin-selected, laboratory susceptible and field-collected aedes aegypti from malaysia. | this study is intended to provide a comprehensive characterization of the resistance mechanisms in the permethrin-selected (imr-pss) and laboratory susceptible (imr-ls) aedes aegypti strain from malaysia. both imr-pss and imr-ls provide a standard model for use in assessing the pyrethroid resistance in field-collected strains collected from three dengue hotspots: the taman seri bayu (tsb), the flat camar (fc), and the taman dahlia (td). two established methods for determining the resistance mech ... | 2018 | 29670071 |
| locomotor activity in males of aedes aegypti can shift in response to females' presence. | the study of physiological and behavioral traits of mosquito vectors has been of growing relevance for the proposition of alternative methods for controlling vector-borne diseases. despite this, most studies focus on the female's traits, including the behavior of host seeking, the physiology of disease transmission and the site-choice for oviposition. however, understanding the factors that lead to males' reproductive success is of utmost importance, since it can help building new strategies for ... | 2018 | 29669591 |
| evaluation of standard field and laboratory methods to compare protection times of the topical repellents pmd and deet. | mosquitoes are important vectors of pathogens, and travellers to disease endemic countries are advised to avoid bites by applying topical repellents. topical repellents are typically tested either in the arm-in-cage (aic) test under laboratory conditions or in the field, but not often under both conditions. we, therefore, investigated how two topical repellents, 15% para-menthane-3,8-diol (pmd) and 15% n,n-diethyl-3-methylbenzamide (deet) compare against each other both in the aic test against t ... | 2018 | 30135603 |
| experimental evaluation of the impact of household aerosolized insecticides on pyrethroid resistant aedes aegypti. | the extensive reliance on insecticides to control aedes aegypti mosquitoes and disrupt transmission of dengue, chikungunya and zika has fueled the emergence of widespread resistance to insecticides. mismatch between the frequency of pyrethroid resistance in mosquitoes and the occurrence of pyrethroid-based insecticide applications for vector control is often hypothesized to be due to household use of commercial insecticide products. we experimentally quantified phenotypic and genotypic responses ... | 2018 | 30135460 |
| differential transmission of antiviral drug-resistant chikungunya viruses by aedes mosquitoes. | the chikungunya virus (chikv) is transmitted by female aedes aegypti and aedes albopictus mosquitoes, mostly present in (sub)tropical regions. no antivirals are available to treat chikv infections. if antiviral drugs are proven efficient to treat chikv-infected patients, it will be pivotal to determine whether drug-resistant viruses can be transmitted from one human to another by their mosquito vectors. we orally infected aedes aegypti mosquitoes with a blood meal containing wild-type or drug-re ... | 2018 | 30135220 |
| study of aedes aegypti population with emphasis on the gonotrophic cycle length and identification of arboviruses: implications for vector management in cemeteries. | aedes aegypti is the vector of the arboviruses causing dengue, chikungunya and zika infections in mexico. however, its presence in public places has not been fully evaluated. in a cemetery from merida, yucatan, mexico, the productivity of ae. aegypti, the gonotrophic cycle, and the presence of ae. aegypti females infected with arboviruses were evaluated. immature and adult mosquitoes were inspected every two months between april 2016 to june 2017. for the gonotrophic cycle length, the daily patt ... | 2018 | 30133604 |