erratum to "comparative oviposition site selection in containers by aedes aegypti and aedes albopictus (diptera: culicidae) from florida". 201829618025
a comparative analysis of corpora allata-corpora cardiaca microrna repertoires revealed significant changes during mosquito metamorphosis.the corpora allata (ca) are a pair of endocrine glands with neural connections to the brain and close association with another neuroendocrine organ, the corpora cardiaca (cc). the ca from adult female aedes aegypti mosquitoes synthesize fluctuating levels of juvenile hormone (jh), which have been linked to the ovarian development and are influenced by nutritional signals. in this study, we investigated the potential involvement of micrornas (mirnas), a type of small non-coding rnas, in the regul ...201829605533
mosquito responses to trait- and density-mediated interactions of predation.mosquito and predatory larvae often share the same habitat. predators may influence mosquito prey populations through both lethal effect and non-lethal pathways. a series of experimental manipulations were used to distinguish between lethal (density-mediated interaction) and non-lethal (trait-mediated interaction) effects in a model system comprised of invasive prey mosquito, aedes aegypti, and a predatory mosquito toxorhynchites rutilus. treatments with predators present or manipulations mimick ...201829594613
effect of relaxation of deltamethrin pressure on metabolic resistance in a pyrethroid-resistant aedes aegypti (diptera: culicidae) strain harboring fixed p989p and g1016g kdr alleles.mutation of the voltage-gated sodium channel genes or knockdown resistance (kdr) and metabolic resistance in aedes aegypti (l.) (diptera: culicidae) are important resistance mechanisms against pyrethroids. the present study investigated the effect of relaxation of deltamethrin selection pressure on the level of mixed-function oxidases (mfo), when the allele frequency of s989p+v1016g mutations is fixed in a resistant ae. aegypti strain (upk-r) from chiang mai, thailand. the mosquitoes were divide ...201829590378
maternal invasion history of aedes aegypti and aedes albopictus into the isthmus of panama: implications for the control of emergent viral disease agents.despite an increase in dengue outbreaks and the arrival of chikungunya and zika disease in panama, studies on the demographic history of the invasive aedes mosquitoes that are the principle vectors of these diseases are still lacking in this region. here, we assess the genetic diversity of these mosquitoes in order to decipher their invasion histories into the isthmus of panama. dna sequences from the mitochondrial cytochrome c oxidase i gene obtained from 30 localities in 10 provinces confirmed ...201829579112
frequency and intensity of pyrethroid resistance through the cdc bottle bioassay and their association with the frequency of kdr mutations in aedes aegypti (diptera: culicidae) from mexico.the control of aedes aegypti (l.), the main urban vector that causes arboviral diseases such as dengue, chikungunya and zika, has proved to be a challenge because of a rapid increase in insecticide resistance. therefore, adequate monitoring of insecticide resistance is an essential element in the control of ae. aegypti and the diseases it transmits. we estimated the frequency and intensity (resistance frequency rapid diagnostic test [f-rdt] and resistance intensity rapid diagnostic test [i-rdt]) ...201829575404
population dynamics of engineered underdominance and killer-rescue gene drives in the control of disease vectors.a number of different genetics-based vector control methods have been proposed. two approaches currently under development in aedes aegypti mosquitoes are the two-locus engineered underdominance and killer-rescue gene drive systems. each of these is theoretically capable of increasing in frequency within a population, thus spreading associated desirable genetic traits. thus they have gained attention for their potential to aid in the fight against various mosquito-vectored diseases. in the case ...201829570717
repellent activity of trig (n-n diethyl benzamide) against man-biting mosquitoes.a study was conducted to assess efficacy of a new repellent brand trig (15% n-n diethyl benzamide) when compared to deet (20% n-n methyl toluamide). the repellents were tested in laboratory and field. in the laboratory, the repellence was tested on human volunteers, by exposing their repellent-treated arms on starved mosquitoes in cages for 3 minutes at hourly intervals, while counting the landing and probing attempts. anopheles gambiae and aedes aegypti mosquitoes were used. field evaluation wa ...201829552040
hr38, an ortholog of nr4a family nuclear receptors, mediates 20-hydroxyecdysone regulation of carbohydrate metabolism during mosquito reproduction.the aedes aegypti mosquito is the principal vector for many dangerous human viral diseases. carbohydrate metabolism (cm) is essential for supplying the energy necessary for host seeking, blood digestion and rapid egg development of this vector insect. the steroid hormone 20-hydroxyecdysone (20e) and the ecdysone receptor (ecr) are important regulators of cm, coordinating it with female reproductive events. we report here that the nr4a nuclear receptor ahr38 plays a critical role in mediating the ...201829526770
jh biosynthesis and hemolymph titers in adult male aedes aegypti mosquitoes.juvenile hormone (jh) is a major hormonal regulator in insects. in aedes aegypti females, jh signals the completion of the ecdysis to the adult stage and initiates reproductive processes. although the regulation of jh synthesis and titer in ae. aegypti females has been extensively studied, relatively little is known about changes of jh synthesis and titers in male mosquitoes, as well as on the roles of jh controlling male reproductive biology. a better understanding of male mosquito reproductive ...201829526769
mosquito pornoscopy: observation and interruption of aedes aegypti copulation to determine female polyandric event and mixed progeny.ades aegypti is the most important arbovirus vector in the world, and new strategies are under evaluation. biological studies mentioning the occurrence of a second mate in aedes aegypti can interfere with vector control program planning, which involves male mosquito release technique. this study presents different experiments to show the occurrence of mixed progeny. mixed male crosses (using a combination of different type of males in confinement with virgin females) showed no polyandric female. ...201829518112
comparative oviposition site selection in containers by aedes aegypti and aedes albopictus (diptera: culicidae) from florida.invasive container mosquito species aedes aegypti (l.) and ae. albopictus (skuse) frequently co-occur in nature where they are typically segregated spatially by distinctive macrohabitat preferences that may select for different oviposition strategies. in a standard cage environment, we compared oviposition site selection by individual gravid females of these species exposed to variable numbers of water-holding cups and the presence or absence of a rugose wettable container lining. offered plasti ...201829514300
building infestation index for aedes aegypti and occurrence of dengue fever in the municipality of foz do iguaçu, paraná, brazil, from 2001 to 2016.the building infestation index (bii) uses the rapid assay of the larval index for aedes aegypti (liraa) to express the relationship between positive and surveyed properties. we evaluated liraa and the relationship between the bii and climate variables for dengue cases in foz do iguaçu municipality, paraná.201829513847
occurrence of sweet refuse at disposal sites: rainwater retention capacity and potential breeding opportunities for aedes aegypti.nectar is the staple diet of adult mosquitoes in the wild, but its availability is inconsistent and can be affected by rainfall. in urban centers, aedes vectors commonly use man-made containers as their major habitat; however, they can colonize any items replenished by rainfall. garbage output has increased significantly in recent years, at a time when collection frequency is reducing. such garbage usually includes organic components, some of which are sweet and can be fed upon by other animals ...201829512008
the non-canonical notch signaling is essential for the control of fertility in aedes aegypti.the notch signaling pathway is a highly evolutionarily-conserved cell-cell signaling pathway that regulates many events during development. it plays a pivotal role in the regulation of fundamental cellular processes, such as cell proliferation, stem cell maintenance, and differentiation during embryonic and adult development. however, functions of notch signaling in aedes aegypti, the major mosquito vector for dengue, are largely unknown. in this study, we identified a unique feature of a. aegyp ...201829505577
dengue knowledge in indoor dengue patients from low socioeconomic class; aetiology, symptoms, mode of transmission and prevention.dengue fever has emerged as an emerging public health issue during last decade bearing significant morbidity and economic burden particularly in third world countries. current study aims to assess various domains of knowledge of indoor dengue patients..201829504327
resistance of aedes aegypti (diptera: culicidae) populations to deltamethrin, permethrin, and temephos in cambodia.dengue fever is a major public health concern, including 185,000 annual cases in cambodia. aedes aegypti is the primary vector for dengue transmission and is targeted with insecticide treatments. this study characterized the insecticide resistance status of ae aegypti from rural and urban locations. the susceptibility to temephos, permethrin, and deltamethrin of ae aegypti was evaluated in accordance with world health organization instructions. all the field populations showed lower mortality ra ...201829502428
bacterial microbiota of aedes aegypti mosquito larvae is altered by intoxication with bacillus thuringiensis israelensis.insect microbiota is a dynamic microbial community that can actively participate in defense against pathogens. bacillus thuringiensis (bt) is a natural entomopathogen widely used as a bioinsecticide for pest control. although bt's mode of action has been extensively studied, whether the presence of microbiota is mandatory for bt to effectively kill the insect is still under debate. an association between a higher tolerance and a modified microbiota was already evidenced but a critical point rema ...201829499735
anti-diuretic action of a capa neuropeptide against a subset of diuretic hormones in the disease vector aedes aegypti.the mosquito aedes aegypti is a vector responsible for transmitting various pathogens to humans, and their prominence as chief vectors of human disease is largely due to their anthropophilic blood feeding behaviour. larval stage mosquitoes must deal with the potential dilution of their haemolymph in freshwater, whereas the haematophagus a. aegypti female faces the challenge of excess ion and water intake after a blood meal. the excretory system, composed of the malpighian tubules (mts) and hindg ...201829496779
dengue in araraquara, state of são paulo: epidemiology, climate and aedes aegypti infestation.objective to describe the epidemiology of dengue in a medium-sized city in the state of são paulo. methods data, such as circulating serotypes, severe cases and deaths, age group, sex, among others, were obtained on reported and confirmed dengue cases in araraquara, state of são paulo, between 1991 and 2015. climatic and infestation data were also analyzed. these variables were evaluated descriptively, using statistical measures such as frequencies, averages, minimum and maximum. dengue incidenc ...201829489994
truck-mounted natular 2ec (spinosad) ulv residual treatment in a simulated urban environment to control aedes aegypti and aedes albopictus in north florida.preemptive treatment of dry habitats with an ultra-low volume (ulv) residual larvicide may be effective in an integrated vector management program to control populations of container-inhabiting aedes mosquitoes, key vectors of zika, dengue, and chikungunya viruses. we exposed dry, artificial containers placed in exposed and protected locations to natular 2ec (spinosad) larvicide applied with a truck-mounted ulv sprayer in a simulated urban setting in north florida, and later introduced water and ...201831442121
ultra-low volume application of spinosad (natular 2ec) larvicide as a residual in a tropical environment against aedes and anopheles species.we investigated the efficacy of a liquid larvicide, natular 2ec® (spinosad), applied with ultra-low volume sprayer as a residual application during the dry season in southeastern thailand against 4 medically important species-aedes aegypti, ae. albopictus, anopheles dirus, and an. minimus. we found that this larvicide could be applied as a residual to dry areas known to collect water and potentially still be effective after rains or irrigation, which could increase the flexibility and efficiency ...201831442120
effect of travel speed on dispersion of aqualuer 20-20 sprayed by a truck-mounted ultra-low-volume sprayer against caged aedes aegypti1.the effect of travel speed of a truck-mounted ultra-low-volume (ulv) sprayer on its application efficacy was studied at st. johns county fairground, elkton, fl, during summer 2015. the efficacy was assessed by spray deposition, droplet size spectrum, and 24-h mortality of caged adult aedes aegypti, using 2 rows of sampling locations, 15 m apart and spread up to 122 m from the spray. each location had a bioassay cage and an impinger droplet sampler, 1 m apart from each other, at 1.5 m off the gro ...201831442115
application efficacy of vectobac wdg against larval aedes aegypti using thermal fog technology.the effectiveness of thermal fog application of bacillus thuringiensis israelensis (vectobac® wdg) against larval aedes aegypti was evaluated in open and forest canopied environments in northeastern florida, using a handheld igeba® tf34 thermal fogger (with water conversion kit). the product was applied at the maximum label rate of 1 kg/ha to linear transects, in each environment, containing empty 0.5-liter cups. after application, late-2nd to early-3rd instars of ae. aegypti were added to each ...201831442110
ultra-low volume application of spinosad (natular 2ec) as a residual in a hot-arid environment against aedes aegypti.the invasive aedes aegypti is an important disease vector increasing in frequency in hot-arid regions of the usa such as the southwest. within hot-arid surroundings this mosquito may be confined to peridomestic locations that tend to be cooler and humid, such as in lush, irrigated ornamental vegetation surrounding homes. however, to reach these habitat refugia, ultra-low volume (ulv) applications of insecticides targeting this mosquito must retain efficacy after being sprayed from the air or str ...201831442108
mosquitocidal activity of a naturally occurring isochroman and synthetic analogs from the plant pathogenic fungus, diaporthe eres against aedes aegypti (diptera: culicidae).the culture filtrate of a plant pathogenic fungus that infects english ivy (hedera helix l., araliaceae) was investigated for mosquitocidal constituents by bioassay-guided isolation. the fungus responsible for pathogenic effects on the plant h. helix has been identified as diaporthe eres nitschke by molecular techniques. the mosquito adulticidal constituent in the culture filtrate was identified as 3,4-dihydro-8-hydroxy-3,5-dimethylisocoumarin (1) by spectroscopic techniques. laboratory bioassay ...201829474636
comparative analysis of major mosquito vectors response to seed-derived essential oil and seed pod-derived extract from acacia nilotica.botanical metabolites are increasingly realized as potential replacements to chemical insecticides. in the present study, acacia nilotica seed essential oil and seed pod solvent extracts were tested for bioefficacy against three important types of mosquitoes. mortality was recorded 24 h post-treatment, while smoke toxicity of adult mosquitoes was recorded at 10 min intervals for 40 min. seed pod powder was extracted with different solvents and hydrodistilled seed oil chemical constituents were d ...201829473901
socioeconomic and human behavioral factors associated with aedes aegypti (diptera: culicidae) immature habitat in tucson, az.aedes aegypti (l.; diptera: culicidae) has been established in the southwestern united states for several decades, but relationships between humans and mosquitoes in this arid region are not well-characterized. in august 2012, the outdoor premises of 355 houses within 20 neighborhoods in tucson, arizona were surveyed for containers that could provide larval habitat for ae. aegypti mosquitoes. at the same time, a knowledge, attitudes and practices (kap) questionnaire was administered to a residen ...201829471405
infectivity of chordodes nobilii larvae (gordiida: nematomorpha).the gordiids are freshwater representatives of the parasite phylum nematomorpha that function asa link between aquatic and terrestrial ecosystems. in recent years, different ecotoxicologic studies have beenmade with the south-american gordiid species, chordodes nobilii, that have demonstrated the capacity of thisgroup to act as a bioindicator of contamination. despite the gordiida’s ecologic relevance, further studies arestill needed to elucidate different aspects of the biology of the class, an ...201729465953
effectiveness of a field trap barrier system for controlling aedes albopictus: a "removal trapping" strategy.aedes aegypti and aedes albopictus are the main vectors for the transmission of several viral pathogens, in particular, dengue, zika and chikungunya. in the absence of vaccines and treatment, control of aedes mosquitoes is the only means of keeping these diseases in check. aedes control is difficult, and it is, therefore, necessary to evaluate the efficacy of novel control methods, particularly those targeting adult and exophilic ae. albopictus populations.201829463300
host plant forensics and olfactory-based detection in afro-tropical mosquito disease vectors.the global spread of vector-borne diseases remains a worrying public health threat, raising the need for development of new combat strategies for vector control. knowledge of vector ecology can be exploited in this regard, including plant feeding; a critical resource that mosquitoes of both sexes rely on for survival and other metabolic processes. however, the identity of plant species mosquitoes feed on in nature remains largely unknown. by testing the hypothesis about selectivity in plant feed ...201829462150
environmental factors can influence dengue reported climate changes directly affect the natural environment and contribute to an increase in the transmission of diseases by vectors. among these diseases, dengue is at the top of the list. the aim of our study was to understand the consequences of temporal variability of air temperature in the occurrence of dengue in an area comprising seven municipalities of the greater são paulo.201729451659
analysis of cluster-randomized test-negative designs: cluster-level methods.intervention trials of vector control methods often require community level randomization with appropriate inferential methods. for many interventions, the possibility of confounding due to the effects of health-care seeking behavior on disease ascertainment remains a concern. the test-negative design, a variant of the case-control method, was introduced to mitigate this issue in the assessment of the efficacy of influenza vaccination (measured at an individual level) on influenza infection. her ...201929447357
current and future distribution of aedes aegypti and aedes albopictus (diptera: culicidae) in who eastern mediterranean region.aedes-borne diseases as dengue, zika, chikungunya and yellow fever are an emerging problem worldwide, being transmitted by aedes aegypti and aedes albopictus. lack of up to date information about the distribution of aedes species hampers surveillance and control. global databases have been compiled but these did not capture data in the who eastern mediterranean region (emr), and any models built using these datasets fail to identify highly suitable areas where one or both species may occur. the ...201829444675
a point mutation v419l in the sodium channel gene from natural populations of aedes aegypti is involved in resistance to λ-cyhalothrin in colombia.resistance to pyrethroids in mosquitoes is mainly caused by target site insensitivity known as knockdown resistance (kdr). in this work, we examined the point mutations present in portions of domains i, ii, iii, and iv of the sodium channel gene in aedes aegypti mosquitoes from three colombian municipalities. a partial region coding for the sodium channel gene from resistant mosquitoes was sequenced, and a simple allele-specific pcr-based assay (as-pcr) was used to analyze mutations at the popul ...201829443870
analysis of peptide ligand specificity of different insect adipokinetic hormone receptors.adipokinetic hormone (akh) is a highly researched insect neuropeptide that induces the mobilization of carbohydrates and lipids from the fat body at times of high physical activity, such as flight and locomotion. as a naturally occurring ligand, akh has undergone quite a number of amino acid changes throughout evolution, and in some insect species multiple akhs are present. akh acts by binding to a rhodopsin-like g protein-coupled receptor, which is related to the vertebrate gonadotropin-releasi ...201829439466
functional characterization and quantitative expression analysis of two gnrh-related peptide receptors in the mosquito, aedes cope with stressful events such as flight, organisms have evolved various regulatory mechanisms, often involving control by endocrine-derived factors. in insects, two stress-related factors include the gonadotropin-releasing hormone-related peptides adipokinetic hormone (akh) and corazonin (crz). akh is a pleiotropic hormone best known as a substrate liberator of proteins, lipids, and carbohydrates. although a universal function has not yet been elucidated, crz has been shown to have roles in ...201829432729
history dependence in insect flight decisions during odor tracking.natural decision-making often involves extended decision sequences in response to variable stimuli with complex structure. as an example, many animals follow odor plumes to locate food sources or mates, but turbulence breaks up the advected odor signal into intermittent filaments and puffs. this scenario provides an opportunity to ask how animals use sparse, instantaneous, and stochastic signal encounters to generate goal-oriented behavioral sequences. here we examined the trajectories of flying ...201829432454
carica papaya (papaya) latex: a new paradigm to combat against dengue and filariasis vectors aedes aegypti and culex quinquefasciatus (diptera: culicidae).this study manifests the larvicidal efficacy of carica papaya latex extract and silver nanoparticles (cpagnps) synthesized using latex, against developing immature juveniles of aedes aegypti and culex quinquefasciatus. briefly, the latex was collected and fractioned with different solvents such as chloroform, methanol and aqueously. the obtained crude extracts were subjected to larvicidal activity in the dose-dependent method. after 24 h, the mortality rate was calculated and statistically analy ...201829430347
re-emergence of aedes aegypti in egypt. 201829412959
entomopathogenic fungi and their potential for the management of aedes aegypti (diptera: culicidae) in the americas.classical biological control has been used extensively for the management of exotic weeds and agricultural pests, but never for alien insect vectors of medical importance. this simple but elegant control strategy involves the introduction of coevolved natural enemies from the centre of origin of the target alien species. aedes aegypti - the primary vector of the dengue, yellow fever and zika flaviviruses - is just such an invasive alien in the americas where it arrived accidentally from its west ...201829412361
the influence of a light and dark cycle on the egg laying activity of aedes aegypti (linnaeus, 1762) (diptera: culicidae).the epidemiological importance of the mosquito aedes aegypti as a vector of multiple human pathogens has generated a growing number of studies on the physiology and behaviour of its blood-feeding females. the activity of oviposition is one of the critical elements contributing to the expansion of ae. aegypti's populations. although there is a vast literature about oviposition behaviour, significant specific knowledge about egg viability and female fertility under light and dark conditions is sti ...201829412343
clinical and epidemiological teaching of dengue through simulated describe the experience of clinical teaching on dengue and the practice of epidemiological surveillance using problematization methodology.201829412306
[eradication of aedes aegypti: new perspectives on international health cooperation in the americas]. 201829412266
context-dependent interactive effects of non-lethal predation on larvae impact adult longevity and body composition.predation impacts development, behavior and morphology of prey species thereby shaping their abundances, distribution and community structure. non-lethal threat of predation, specifically, can have a strong influence on prey lifehistory characteristics. while investigations often focus on the impact of predation threat on prey in isolation, tests of its interactive effects with food availability and resource competition on prey survival and fitness can improve understanding of costs, benefits an ...201829401513
molecular identification, transcript expression, and functional deorphanization of the adipokinetic hormone/corazonin-related peptide receptor in the disease vector, aedes aegypti.the recently discovered adipokinetic hormone/corazonin-related peptide (acp) is an insect neuropeptide structurally intermediate between corazonin (crz) and adipokinetic (akh) hormones, which all demonstrate homology to the vertebrate gonadotropin-releasing hormone (gnrh). to date, the function of the acp signaling system remains unclear. in the present study, we molecularly identified the complete open reading frame encoding the aedes aegypti acp receptor (acpr), which spans nine exons and unde ...201829391531
[the recent epidemic spread of zika virus disease].zika virus (zikv) is one of the members of the spondweni serocomplex within the genus flavivirus of the family flaviviridae. the virus was first isolated from a serum specimen from a sentinel non-human primate in the zika forest of uganda in 1947. zikv is transmitted by aedes aegypti and a. albopictus in an urban cycle and maintained in a sylvatic cycle between aedes mosquitoes and monkeys in africa and asia. initially, the virus was thought to cause only mild and nonspecific clinical symptoms i ...201831105130
[coinfection of dengue and chikungunya viruses. narrative review].dengue and chikungunya viruses are transmitted by the female aedes aegypti and aedes albopictus, which are widely distributed in tropical and subtropical areas, facilitating coinfection.201931095186
[current distribution and bioecological characterizations of aedes aegypti and aedes albopictus in brazzaville].aedes aegypti is a mosquito that has been known in congo for decades. it has always been involved in the transmission of some arboviruses. ae. albopictus is an invasive species native from asia. its presence was reported in congo in 2009. the proliferation of ae. albopictus, especially in brazzaville, in association with ae. aegypti, have increased the risk of arbovirus transmission. the distribution and bio-ecology of these two species remain poorly studied. we undertook entomological investiga ...201830950593
[clinical and epidemiological profile of chikungunya infection in hospital patients in 2015 in mérida, mexicoperfil clínico-epidemiológico da infecção por chikungunya em casos hospitalares atendidos em 2015 em mérida, méxico].describe the epidemiological profile and analyze clinical manifestations by age group in chikungunya patients treated in 2015 in a mérida, mexico general hospital.201731391823
erratum: efficacy of some wearable devices compared with spray-on insect repellents for the yellow fever mosquito, aedes aegypti (l.) (diptera: culicidae). 201728365772
tropical viruses approaching europe.during the last years, headlines about the advance of tropical infectious diseases, which are transmitted by mosquitos, become more frequent. some of these mosquitos are classified as invasive species in europe. the world health organization (who) and the european center for disease prevention and control (ecdc) are enhancing the observation of the geographical spread of these species. especially the asian tiger mosquito and the yellow fever mosquito are in focus. in germany the vector competenc ...201729952164
tropical viruses approaching europe.during the last years, headlines about the advance of tropical infectious diseases, which are transmitted by mosquitos, become more frequent. some of these mosquitos are classified as invasive species in europe. the world health organization (who) and the european center for disease prevention and control (ecdc) are enhancing the observation of the geographical spread of these species. especially the asian tiger mosquito and the yellow fever mosquito are in focus. in germany the vector competenc ...201729952163
zika virus disease.the zika virus is an arbovirus belonging to the virus family flaviviridae. the virus was isolated in 1947 from a rhesus monkey in the zika forest of uganda. the virus causes sporadic mild human infections in africa and later in asia. however, by 2007 a major shift in its infection pattern was noticed and thousands of human infections were reported in the state of yap and federated states of micronesia. in the last 3 years, major outbreaks have continued to occur and the virus has spread to sever ...201930787745
[(assessment of risk for arbovirus infections in russia)].the sharply increased incidence and geographical expansion of arbovirus diseases in recent years suggest that some russian areas are vulnerable to vector-borne diseases and that it is important to elaborate control programs to ensure bio- logical safety in our country. moreover, the main vectors of the pathogens of arbovirus infections (zika fever, dengue fever, chikungunya fevers), such as aedes aegypti and ae.albopictus, have been registered on the black sea coast of the caucasus. the programs ...201730721608
larvicidal and pupicidal activities of crude and fractionated extracts of acacia pennata (l.) willd. subsp insuavis shoot tips against aedes aegypti (l.) (diptera: culicidae).acacia pennata subsp insuavis, or cha-om in thai, is a common vegetablefound in thailand. it has been used as a medicinal herb for a long time. from theliterature, antinociceptive, anti-inflammatory, antimicrobial, and anti-helminthicactivities were reported. in this study, we investigated two new actions of thisplant: larvicide and pupicide. the crude ethanolic and fractionated extracts of a.pennata shoot tips were tested against aquatic stages of the dengue virus vector,aedes aegypti mosquito. ...201729644817
[updating the larval habitats of aedes aegypti (diptera: culicidae) in camagüey, cuba].several illnesses of vectorial origin have a great medical and veterinary relevance, due to the adaptations developed by the species involved in their transmission. to support preventive programs with updated ecological information of ae. aegypti populations, an entomological survey was carried out in three health areas of camagüey, cuba, during low and high rain seasons of 2013. for this, we assessed the type and number of positive containers, quantified larvae and pupae in positive containers, ...201629465911
chemical composition, antioxidant, antimicrobial and insecticidal activities of essential oil from a moroccan endemic plant: bubonium imbricatum.the chemical composition, antioxidant, antimicrobial and insecticidal properties of essential oil obtained by hydro-distillation from bubonium imbricatum cav. have been investigated. the essential oil of aerial parts was analyzed by gc-fid and gc-ms. a total of 63 compounds were identified representing more than 82% of the oil. the major components were cis-chrysanthenyl acetate (31.2%) and thymol isobutyrate (3.4%). the antioxidant activity was evaluated by the dpph and reducing power test. the ...201630475515
lantana montevidensis essential oil: chemical composition and mosquito repellent activity against aedes aegypti.the essential oil (eo) of lantana montevidensis (spreng.) briq. (l. sellowiana link & otto) was investigated for its chemical composition and mosquito repellent activity. the essential oil obtained by hydrodistillation of aerial plant parts was analyzed by gc-fid and gc-ms. the major constituents were p-elemene (22.0%), β-caryophyllene (20.1%), and germacrene d (9.4%). sesquiterpene hydrocarbons were considerable quantities (78.9%) in the l. montevidensis eo, followed by oxygenated se ...201630475514
toxicity of compounds isolated from white snakeroot (ageratina altissima) to adult and larval yellow fever mosquitoes (aedes aegypti).botanicals have been the source for several classes of pesticides for mosquitoes. however, increasing resistance to these products and reduced numbers of choices necessitate the search for new active ingredients. ageratina altissima (l.) king & h. rob. or white snakeroot, is found throughout the eastern united states and contains known toxic compounds. we examine chromenes (benzopyrans) and benzofurans isolated fiom this plant for larvicidal and adulticidal activity against the dengue vector aed ...201630475505
preparative capillary gc for characterization of five dracocephalum essential oils from mongolia, and their mosquito larvicidal activity.the chemical composition and mosquito larvicidal and adulticidal activity of five essential oils (eos), dracocephalum ruyschiana l. (dr), d. foetidum bunge (df), d. moldavica l. (dm), d. fruticulosum steph. ex willd. (dfr) and d. peregrinum l. (dp) were evaluated. simultaneous gc-fid and gc-ms analyses revealed in the eos of dr, df and dm an unidentified compound (1) ([m'122], 5.4%, 57.9% and 74.0%, respectively). therefore, we aimed to isolate compound 1 using preparative capillary gc (pcgc) co ...201630549618
chemical composition and biting deterrent activity of essential oil of tagetes patula (marigold) against aedes aegypti.the hydrodistilled leaf essential oil (eo) of tagetes patula l. was analyzed by gc-fid and gc-ms. the main components of the oil were characterized as caryophyllene oxide (18.4%), p-caryophyllene (18.0%) and spathulenol (9.1%). the eo was screened for its biting deterrent activity against aedes aegypti l. using the in vitro k&d module system. t. patula eo exhibited good biting deterrent activity. the results suggest that these sesquiterpenes may contribute to biting deterrent activity, but the r ...201630549616
essential oil composition of pimpinella cypria and its insecticidal, cytotoxic, and antimicrobial activity.a water-distilled essential oil (e) from the aerial parts of pimpinella cypria boiss. (apiaceae), an endemic species in northern cyprus, was analyzed by gc- fid and gc-ms. forty-five compounds were identified in the oil, which comprised 81.7% of the total composition. the compound classes in the oil were oxygenated sesquiterpenes (33.9%), sesquiterpenes (22.0%), monoterpenes (11.4%), oxygenated monoterpenes (2.6%), and phenylpropanoids (7.5%). the main components of the oil were (z)-β-farnesene ...201630549615
11-hydroxy-2,4-cycloeudesmane from the leaf oil of juglans regia and evaluation of its larvicidal activity.leaves of juglans regia l. collected from two different locations [adana (a) and ankara (b)] from turkey were subjected to hydrodistillation in a clevenger type apparatus to yield the essential oils (eos). gc/ms and gc-fid analyses revealed that the a eo was rich in thymol (23.1%), while caryophyllene oxide (33.8%) was found as the main constituent of b eo. both contained p-eudesmol (1.4% - 9.5 %), (e)-geranyl acetone (3.7% - 5.8%) and the eudesmane type constituent juglaterpene a (3.1% - 11.0%) ...201630549590
repellent and insecticidal efficacy of a combination of dinotefuran, pyriproxyfen and permethrin (vectra® 3d) against culex pipiens in dogs.culex pipiens is an important vector of pathogens of substantial medical and veterinary importance such as dirofilaria immitis and dirofilaria repens or the west nile virus. the control of these mosquitoes is therefore essential to control the transmission of mosquito-borne agents to humans and animals. a combination of dinotefuran, permethrin and pyriproxyfen (vectra® 3d) has already shown its efficacy against aedes aegypti. the aim of this study was to confirm the efficacy of this combination ...201629988200
[construction of aaldv-3 mediated mirrna sponge delivery system and its effect on cell and larva of aedes aegypti].micrornas (mirnas) not only play the key roles in regulation of the growth and development of mosquito, but also has an important function in interaction between the pathogen and vector. so, mirnas can be used as the molecular target for development of an alternative method for mosquito and mosquito-borne disease control. however, an effective delivery system is still need. mosquito densovirus have the potential for vector control as transducing agents to express foreign toxins or small interfer ...201629979549
bioefficacy of ecbolin a and ecbolin b isolated from ecbolium viride (forsk.) alston on dengue vector aedes aegypti l. (diptera: culicidae).ecbolin a and ecbolin b were isolated from ethyl acetate extract of ecbolium viride (forsk.) alston root and evaluated for larvicidal and growth disturbance activities against aedes aegypti l. (diptera: culicidae). for larvicidal activity, the third instar larvae of a. aegypti were exposed to different concentrations viz., 1.0, 2.5, 5.0 and 10 ppm for each compound. among the two compounds screened, ecbolin b recorded highest larvicidal activity with lc50 and lc90 values of 0.70 and 1.42 ppm, re ...201629988190
effect of gamma radiation on life history traits of aedes aegypti (l.).aedes aegypti is an important vector for dengue and dengue hemorrhagic fever. considering its medical importance and its relevance as a model system, this study was undertaken to evaluate the impact of different doses of gamma radiation for three generations of a. aegypti. two to three days old virgin males of a. aegypti were irradiated with 15 doses of gamma radiation, ranging from 1 to 50 gy and were immediately mass mated with the same aged virgin females. observations were made for changes o ...201629988174
[identification and expression of the yellow gene family in aedes aegypti].to identify the yellow family genes in aedes aegypti and analyze the gene structure, phylogenetic evolution and their expression at various developmental stages and in different tissues.201630120914
is there a risk of chikungunya transmission in canada?an outbreak of chikungunya virus infection is currently underway in the caribbean, central america and south america, and autochthonous (local or indigenous) transmission has occurred in the southeastern united states. the mosquito species known to transmit chikungunya-aedes aegypti and aedes albopictus-are not known to reside in canada at this time. but how comfortable can we be that this situation will continue? here we explore four key conditions that must be met for transmission of chikungun ...201529769915
exploring the relationships between dengue fever knowledge and aedes aegypti breeding in st catherine parish, jamaica: a pilot of enhanced low-cost surveillance.dengue fever has re-emerged as an increasingly significant global health threat amid diminishing resources pledged for its control in developing nations. efforts to limit breeding of the dengue vector aedes aegypti are often hampered by lack of community awareness of the disease.201130881182
changes in the fat body and oocysts during starvation and vitellogenesis in a mosquito, aedes aegypti (l.).when aedes aegypti females first emerge as adults, their oocytes possess no yolk. the abdominal fat body cells contain large quantities of lipid, protein, and glycogen, and possess many free ribosomes, but have very little rough endoplasmic reticulum (rer). when the females are starved for four days, their oocytes form fine lipid and protein yolk endogenously, the latter being located mainly around the nucleus. the adipocytes in these fasted mosquitoes have greatly reduced amounts of lipid, prot ...197930189706
fine structure of tarsal sensilla of aedes aegypti (l.) (diptera: culicidae).the tarsi of all three pairs of legs of both sexes of aedes aegypti (l.) bear spine sensilla, five types of hair sensilla, which are designated a, b, c1, c2 and c3, and campaniform sensilla. type a and b hairs, spines, and cam-paniform sensilla are innervated by one neuron with a tubular body, a characteristic of cuticular mechanoreceptors. in particular the hairs and spines are tactile receptors and the campaniform sensilla are proprioceptors. the c1, c2, and c3 hair sensilla have the morpholog ...197830227696
ultrastructure of the peg and hair sensilla on the antenna of larval aedes aegypti (l.).the antenna of fourth instar larvae of aedes aegypti has one peg organ of a basiconic type innervated by four neurons. the dendrites are ensheathed to near their terminations at the peg tip by an electron-dense dendritic sheath and by a cuticular sheath. they have easy communication by diffusion with the external environment only at the tip through a peripheral ensheathing membrane and six slit-channels. one of the dendrites resembles a tubular body proximally and may be mechanoreceptive. the pe ...197130380807
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