follow up of a robust meta-signature to identify zika virus infection in aedes aegypti: another brick in the wall.the mosquito aedes aegypti is the main vector of several arthropod-borne diseases that have global impacts. in a previous meta-analysis, our group identified a vector gene set containing 110 genes strongly associated with infections of dengue, west nile and yellow fever viruses. of these 110 genes, four genes allowed a highly accurate classification of infected status. more recently, a new study of ae. aegypti infected with zika virus (zikv) was published, providing new data to investigate wheth ...201829846381
the use of wolbachia by the world mosquito program to interrupt transmission of aedes aegypti transmitted viruses.the biological control of mosquito transmission by the use of the naturally occurring insect-specific bacterial endosymbiont wolbachia has been successfully tested in small field trials. the approach has been translated successfully to larger field sites in townsville, australia and expanded to more than 10 countries through the eliminate dengue program. the broader application of the program beyond limiting the transmission of dengue and including other aedes aegypti borne mosquitoes has seen t ...201829845544
structures of zika virus e & ns1: relations with virus infection and host immune responses.zika virus (zikv), first discovered in the zika forest in uganda in 1947 was understudied until the recent explosive epidemic in several south american countries where it has become strongly associated with congenital birth defects leading to severe cranial malformations and neurological conditions. the increase in number of case of microcephaly in newborn children associated with zikv infection triggered the world health organization to declare the epidemic as a public health emergency of inter ...201829845526
back-to-the-future potential for autochthonous transmission of aedes aegypti-transmitted viruses in ethekwini and urban coastal kwazulu-natal province, south africa. 201829843848
the risk of urban yellow fever resurgence in aedes-infested american cities.aedes aegypti, historically known as yellow fever (yf) mosquito, transmits a great number of other viruses such as dengue, west nile, chikungunya, zika, mayaro and perhaps oropouche, among others. well established in africa and asia, aedes mosquitoes are now increasingly invading large parts of the american continent, and hence the risk of urban yf resurgence in the american cities should because of great concern to public health authorities. although no new urban cycle of yf was reported in the ...201829843824
evaluation of larvicidal, adulticidal, and anticholinesterase activities of essential oils of illicium verum hook. f., pimenta dioica (l.) merr., and myristica fragrans houtt. against zika virus vectors.aedes aegypti is the vector responsible for transmitting pathogens that cause various infectious diseases, such as dengue, zika, yellow fever, and chikungunya, worrying health authorities in the tropics. due to resistance of mosquitoes to synthetic insecticides, the search for more effective insecticidal agents becomes crucial. the aim of this study was to verify the larvicidal, adulticidal, and anticholinesterase activities of the essential oils of the illicium verum (eoiv), pimenta dioica (eop ...201829808407
rapid, noninvasive detection of zika virus in aedes aegypti mosquitoes by near-infrared spectroscopy.the accelerating global spread of arboviruses, such as zika virus (zikv), highlights the need for more proactive mosquito surveillance. however, a major challenge during arbovirus outbreaks has been the lack of rapid and affordable tests for pathogen detection in mosquitoes. we show for the first time that near-infrared spectroscopy (nirs) is a rapid, reagent-free, and cost-effective tool that can be used to noninvasively detect zikv in heads and thoraces of intact aedes aegypti mosquitoes with ...201829806030
impact of short-term temperature challenges on the larvicidal activities of the entomopathogenic watermold leptolegnia chapmanii against aedes aegypti, and development on infected dead larvae.the oomycete leptolegnia chapmanii is among the most promising entomopathogens for biological control of aedes aegypti. this mosquito vector breeds in small water collections, where this aquatic watermold pathogen can face short-term scenarios of challenging high or low temperatures during changing ambient conditions, but it is yet not well understood how extreme temperatures might affect the virulence and recycling capacities of this pathogen. we tested the effect of short-term exposure of ency ...201829801786
vapor toxicity of five volatile pyrethroids against aedes aegypti, aedes albopictus, culex quinquefasciatus, and anopheles quadrimaculatus (diptera: culicidae).mosquito mortality has been documented in numerous studies of spatial repellents but the concentration-dependent toxicity of spatial repellent vapors has not been documented. to address this issue, prallethrin, flumethrin, metofluthrin, transfluthrin, and meperfluthrin were selected for comparative study against aedes albopictus (skuse), ae. aegypti (l.), culex quinquefasciatus say, and anopheles quadrimaculatus say. mosquito were exposed to vapors of each chemical for 2, 4, and 24 h with mortal ...201829797795
codon adaptation biases among sylvatic and urban genotypes of dengue virus type 2.dengue virus (denv) emerged from the sylvatic environment and colonized urban settings, being sustained in a human-aedes-human transmission chain, mainly by the bites of females of the anthropophilic species aedes aegypti. herein, we sought evidence for fine-tuning in viral codon usage, possibly due to viral adaptation to human transmission. we compared the codon adaptation of denv serotype 2 (denv-2) genotypes from urban and sylvatic habitats and tried to correlate the findings with key evoluti ...201829792991
surveillance of mosquitoes (diptera: culicidae) in southern iowa, 2016.the mosquito fauna of iowa has been extensively investigated over several decades, providing a wealth of information regarding species distributions, relative abundance, temporal activity patterns, and identifying vectors of medical importance. however, these investigations have had unequal coverage, leaving the mosquito fauna in some parts of the state, including southern iowa, largely uncharacterized. with the heightened public health threat of zika virus in the summer of 2016, greater emphasi ...201829788488
threshold dynamics of a temperature-dependent stage-structured mosquito population model with nested delays.mosquito-borne diseases remain a significant threat to public health and economics. since mosquitoes are quite sensitive to temperature, global warming may not only worsen the disease transmission case in current endemic areas but also facilitate mosquito population together with pathogens to establish in new regions. therefore, understanding mosquito population dynamics under the impact of temperature is considerably important for making disease control policies. in this paper, we develop a sta ...201829785519
a multi-faceted pandemic: a review of the state of knowledge on the zika virus.while until recently the small and isolated zika outbreaks in eastern asia and pacific islands had been overlooked, the large-scale outbreak that started in brazil in 2015 and the increase of microcephaly cases in the same place and time made media headlines. considered as harmless until recently, zika has given rise to an important global crisis that poses not only health challenges but also environmental, economical, social, and ethical challenges for states and people around the world. the ma ...201829785319
efficacy of lysinibacillus sphaericus against mixed-cultures of field-collected and laboratory larvae of aedes aegypti and culex quinquefasciatus.lysinibacillus sphaericus (bacillales: planococcaceae) is a spore-forming bacillus used for the biological control of mosquitoes (diptera: culicidae) due to its larvicidal activity determined by various toxins and s-layer protein produced either during sporulation or by the vegetative cell. aedes aegypti and culex quinquefasciatus are the vectors of arboviruses that cause tropical diseases representing a current public health problem. both species may coexist in the same larval development sites ...201929784071
regulation of midgut cell proliferation impacts aedes aegypti susceptibility to dengue virus.aedes aegypti is the vector of some of the most important vector-borne diseases like dengue, chikungunya, zika and yellow fever, affecting millions of people worldwide. the cellular processes that follow a blood meal in the mosquito midgut are directly associated with pathogen transmission. we studied the homeostatic response of the midgut against oxidative stress, as well as bacterial and dengue virus (denv) infections, focusing on the proliferative ability of the intestinal stem cells (isc). i ...201829782512
zika virus: report from the task force on tropical diseases by the world federation of societies of intensive and critical care medicine.zika fever is an acute infectious disease caused by the zika virus (zikv), an rna virus belonging to the genus flavivirus. zikv can be transmitted by bites from aedes aegypti and aedes albopictus species, sexual activity, blood transfusion and from mother to fetus during pregnancy and delivery. a total of 50 territories and countries in the americas had reported zikv infections. for those with symptoms, the presentation is similar to dengue fever with body aches, joint pain, fatigue, malaise, fe ...201829779827
genome sequence analysis of a novel bacillus thuringiensis strain blb406 active against aedes aegypti larvae, a novel potential bioinsecticide.blb406 is a novel isolate of bacillus thuringiensis with a larvicidal activity against aedes aegypti larvae. it displays original plasmidic and crystal protein patterns. the present work reported molecular and bioinformatic analyses for the genome sequence of blb406 using miseq illumina next-generation sequencing technology. the reads were assembled by velvet tool. using rast program and pgaap, the genome of blb406 strain was shown to contain 6297 genes corresponding to 5924 protein coding seque ...201829778876
chemical profile and pharmacological effects of the resin and essential oil from bursera slechtendalii: a medicinal "copal tree" of southern mexico.this study reports on the volatile profile and pharmacological activities of the essential oil and resin from bursera slechtendalii, an aromatic medicinal tree commercialized in southern mexico. the essential oil contained 25 volatiles including p-cymen-8-ol (25.4-35.7%), n-hexyl hexanoate (6.8-17.6%) and n-octyl hexanoate (7.6-14.8%), whereas the crystallized resin contained 14 volatiles, including n-octyl acetate (29.6-34.2%), limonene (15.8-18.7%) and n-octyl butyrate (12.6-17-6%). the inhala ...201829777752
modeling dengue vector population using remotely sensed data and machine learning.mosquitoes are vectors of many human diseases. in particular, aedes ægypti (linnaeus) is the main vector for chikungunya, dengue, and zika viruses in latin america and it represents a global threat. public health policies that aim at combating this vector require dependable and timely information, which is usually expensive to obtain with field campaigns. for this reason, several efforts have been done to use remote sensing due to its reduced cost. the present work includes the temporal modeling ...201829777650
use of bead-based serologic assay to evaluate chikungunya virus epidemic, haiti.the index case of chikungunya virus (chikv) in haiti was reported during early 2014; the vector, the pervasive aedes aegypti mosquito, promoted rapid spread throughout the country. during december 2014-february 2015, we collected blood samples from 4,438 persons at 154 sites (62 urban, 92 rural) throughout haiti and measured chikv igg by using a multiplex bead assay. overall chikv seroprevalence was 57.9%; differences between rural (mean 44.9%) and urban (mean 78.4%) areas were pronounced. logis ...201829774861
evaluation of phytosynthesised silver nanoparticles from leaf extracts of leucas aspera and hyptis suaveolens and their larvicidal activity against malaria, dengue and filariasis vectors.the present study deals with the green synthesis of silver nanoparticle from the aqueous leaf extracts of leucas aspera and hyptis suaveolens as reducing agent and to investigate the larvicidal activity of synthesized silver nanoparticles. the synthesized silver nanoparticles were characterized by ultraviolet and visible absorption spectroscopy (uv), fourier transform-infrared spectroscopy (ft-ir), x-ray spectroscopy (xrd), field emission scanning electron microscope (fesem) and high-resonance t ...201729774292
biological control of anopheles darlingi, aedes aegypti and culex quinquefasciatus larvae using shrimps.mosquitoes can act as vectors of important diseases such as malaria, dengue, zika virus, yellow fever, chikungunya and mayaro fever, in addition to filariasis. the use of insecticides, larvicides, bed nets and repellents, besides the use of drugs as chemoprevention and the treatment of the sick are currently the pillars of the control of these vectors. we studied the biological control against of anopheles darlingi, aedes aegypti and culex quinquefasciatus larvae using shrimps of the species m. ...201729774286
the influence of urban heat islands and socioeconomic factors on the spatial distribution of aedes aegypti larval habitats.we addressed the potential associations among the temporal and spatial distribution of larval habitats of aedes (stegomyia) aegypti, the presence of urban heat islands and socioeconomic factors. data on larval habitats were collected in santa bárbara d'oeste, são paulo, brazil, from 2004 to 2006, and spatial and temporal variations were analysed using a wavelet-based approach. we quantified urban heat islands by calculating surface temperatures using the results of wavelet analyses and grey leve ...201829772891
urban environmental clustering to assess the spatial dynamics of aedes aegypti breeding sites.the identification of aedes aegypti breeding hotspots in urban areas is crucial for the rational design of control strategies against this disease vector. remote sensing and geographic information systems offer valuable tools for mapping habitat suitability of a given area. however, predicting species occurrences by means of probability distribution maps based on transversal entomological surveys has limited utility for local authorities. the aim of the present study was to carefully examine the ...201829772886
encapsidated host factors in alphavirus particles influence midgut infection of aedes aegypti.transmission of mosquito-borne viruses requires the efficient infection of both a permissive vertebrate host and a competent mosquito vector. the infectivity of sindbis virus (sinv), the type species of the alphavirus genus, is influenced by both the original and new host cell. we have shown that infection of vertebrate cells by sinv, chikungunya virus (chikv), and ross river virus (rrv) produces two subpopulations of virus particles separable based on density. in contrast, a single population o ...201829772674
tracking aedes aegypti (diptera: culicidae) larval behavior across development: effects of temperature and nutrients on individuals' foraging behavior.immature mosquitoes alter their foraging behavior in response to variation in nutrients, predators, and temperature, with consequences on the adult stage where pathogens are transmitted. these patterns of behavior have not been described with respect to both developmental stage and environmental variation, nor has behavior been examined within an individual across instars. we hypothesized that individual larvae have distinct behavioral syndromes, and predict that the rank of foraging activity in ...201829771372
niche conservatism of aedes albopictus and aedes aegypti - two mosquito species with different invasion histories.biological invasions have been associated with niche changes; however, their occurrence is still debated. we assess whether climatic niches between native and non-native ranges have changed during the invasion process using two globally spread mosquitoes as model species, aedes albopictus and aedes aegypti. considering the different time spans since their invasions (>300 vs. 30-40 years), niche changes were expected to be more likely for ae. aegypti than for ae. albopictus. we used temperature a ...201829769652
ornamental bromeliads of miami-dade county, florida are important breeding sites for aedes aegypti (diptera: culicidae).a major public health concern is the emergence and geographical spread of vector-borne diseases such as zika and yellow fever. ornamental bromeliads retaining water in their leaf axils represent potential breeding sites for mosquitoes. as the role of ornamental bromeliads in breeding aedes aegypti in miami-dade county, florida is unknown, we hypothesize that ornamental bromeliads are important breeding sites for ae. aegypti. our objective was to survey bromeliads in areas with high densities of ...201829769105
efficacy of the main repellents available in the brazilian market against aedes aegypti bites under concentrations applied to pediatric populations. 201829768569
ns1 codon usage adaptation to humans in pandemic zika virus.zika virus (zikv) was recognised as a zoonotic pathogen in africa and southeastern asia. human infections were infrequently reported until 2007, when the first known epidemic occurred in micronesia. after 2013, the asian lineage of zikv spread along the pacific islands and americas, causing severe outbreaks with millions of human infections. the recent human infections of zikv were also associated with severe complications, such as an increase in cases of guillain-barre syndrome and the emergenc ...201829768530
pyrethroid insecticides maintain repellent effect on knock-down resistant populations of aedes aegypti mosquitoes.pyrethroid-treated clothing is commonly worn for protection against mosquitoes; pyrethroids are both insecticides and repellents. pyrethroid resistance has become increasingly common in aedes aegypti, the vector of dengue, zika, and other arboviruses, but it is not clear whether resistance is associated with reductions in repellency. in order to determine whether long-lasting permethrin impregnated (llpi) clothing is protective, we used aedes aegypti from new orleans, la (pyrethroid-sensitive) a ...201829763445
effects of light and temperature on daily activity and clock gene expression in two mosquito disease vectors.most organisms feature an endogenous circadian clock capable of synchronization with their environment. the most well-known synchronizing agents are light and temperature. the circadian clock of mosquitoes, vectors of many pathogens, drives important behaviors related to vectoral capacity, including oviposition, host seeking, and hematophagy. main clock gene expression, as well as locomotor activity patterns, has been identified in aedes aegypti and culex quinquefasciatus under artificial light- ...201829761749
threats of zika virus transmission for asia and its hindu-kush himalayan and its hindu kush himalayan (hkh) region is particularly vulnerable to environmental change, especially climate and land use changes further influenced by rapid population growth, high level of poverty and unsustainable development. asia has been a hotspot of dengue fever and chikungunya mainly due to its dense human population, unplanned urbanization and poverty. in an urban cycle, dengue virus (denv) and chikungunya virus (chikv) are transmitted by aedes aegypti and ae. albopictus mosqui ...201829759076
aedes aegypti(linnaeus) larvae from dengue outbreak areas in selangor showing resistance to pyrethroids but susceptible to organophosphates.the resistance status of selangor aedes aegypti (linnaeus) larvae against four major groups of insecticides (i.e., organochlorines, carbamates, organophosphates and pyrethroids) was investigated. aedes aegypti were susceptible against temephos (organophosphate), although resistance (rr50 = 0.21-2.64) may be developing. the insecticides susceptibility status of ae. aegypti larvae were found heterogeneous among the different study sites. results showed that ae. aegypti larvae from klang, sabak ber ...201829758171
a review of the ongoing research on zika virus treatment.the zika fever is an arboviral disease resulting from the infection with zika virus (zikv). the virus is transmitted to humans by the bite of aedes mosquitos, mainly aedes aegypti and aedes albopictus. zikv has been detected for decades in african and asian regions and, since 2007, has spread to other continents; among them, infections are most reported in the americas. this can be explained by the presence of vectors in highly populated and tropical regions where people are susceptible to conta ...201829758005
two novel mutations in the voltage-gated sodium channel associated with knockdown resistance (kdr) in the dengue vector aedes aegypti in vietnam. 201829757514
diel patterns of aedes aegypti (diptera: culicidae) after resurgence in st. augustine, florida as collected by a mechanical rotator trap. 201829757509
mosquito traps for urban surveillance: collection efficacy and potential for use by citizen scientists.mosquito-borne diseases are a pervasive public health problem on a global scale, and effective management of them requires well-designed surveillance programs for both vectors and pathogens. mosquito traps are a common component of such programs, and their reach can be expanded by engaging citizen scientists. in this study in a southern australian city, we compared the mosquito collection efficacy of two types of traps and assessed their suitability for use in citizen science programs. bg sentin ...201829757507
controversies over the scientific name of the principal mosquito vector of yellow fever virus - expediency versus validity.the history of the scientific name of the yellow fever mosquito, the vector of yellow fever virus, ranges from 1757 to the early twenty-first century. in his 1757 work iter palaestinum, frederic hasselquist gave the name culex aegypti to a mosquito species responsible for fierce attacks on humans in egypt. that name was never later ascribed to hasselquist as author, but to linnaeus, although the name never appeared in any of linnaeus' publications. in cuba, at the end of the nineteenth century, ...201829757506
effects of scarcity and excess of larval food on life history traits of aedes aegypti (diptera: culicidae).few studies have assessed the effects of food scarcity or excess on the life history traits of aedes aegypti (l.) (diptera: culicidae) independently from larval density. we assessed immature survival, development time, and adult size in relation to food availability. we reared cohorts of 30 ae. aegypti larvae from newly hatched to adult emergence with different food availability. food conditions were kept constant by transferring larvae each day to a new food solution. immature development was c ...201829757503
de novo dna synthesis in aedes aegypti midgut cells as a complementary strategy to limit dengue viral replication.aedes aegypti is the main vector of dengue virus, carrying the virus during the whole mosquito life post-infection. few mosquito fitness costs have been associated to the virus infection, thereby allowing for a swift dissemination. in order to diminish the mosquito population, public health agency use persistent chemicals with environmental impact for disease control. most countries barely use biological controls, if at all. with the purpose of developing novel dengue control strategies, a detai ...201829755433
sargassum wightii-synthesized zno nanoparticles - from antibacterial and insecticidal activity to immunostimulatory effects on the green tiger shrimp penaeus semisulcatus.the green synthesis of metal nanoparticles using phytochemical from marine seaweeds is a fast-growing research field in nanotechnology. here, the biosynthesis of zinc oxide nanoparticles was achieved using the hot water extract of sargassum wightii. the hot water extract prepared from s. wightii (h sw) and zno nps were studied by uv-visible and ftir spectroscopy, sem and xrd. then, both products were evaluated for antibiofilm activity towards aquatic pathogens. the nanoparticles' immunostimulati ...201829754050
dynamics of dengue disease with human and vector mobility.dengue is a vector borne disease transmitted to humans by aedes aegypti mosquitoes carrying virus of different serotypes. dengue exhibits complex spatial and temporal dynamics, influenced by various biological, human and environmental factors. in this work, we study the dengue spread for a single serotype (denv-1) including statistical models of human mobility with exponential step length distribution, by using reaction-diffusion equations and stochastic cellular automata (sca) approach. we anal ...201829751893
a wolbachia triple-strain infection generates self-incompatibility in aedes albopictus and transmission instability in aedes aegypti.artificially-introduced transinfections of the intracellular bacterium wolbachia pipientis have the potential to reduce the vectorial capacity of mosquito populations for viruses such as dengue and chikungunya. aedes albopictus has two native strains of wolbachia, but their replacement with the non-native wmel strain blocks transmission of both viruses. the pattern of cytoplasmic incompatiiblity generated by wmel with wild-types is bidirectional. novel-plus-native-strain co-infection is predicte ...201829751814
molecular responses to the zika virus in mosquitoes.the zika virus (zikv), originally discovered in 1947, did not become a major concern until the virus swept across the pacific and into the americas in the last decade, bringing with it news of neurological complications and birth defects in zikv affected areas. this prompted researchers to dissect the molecular interactions between zikv and the mosquito vector in an attempt to better understand not only the changes that occur upon infection, but to also identify molecules that may potentially en ...201829751526
effect of uv-b irradiation on water-suspended metarhizium anisopliae s.l. (hypocreales: clavicipitaceae) conidia and their larvicidal activity in aedes aegypti (diptera: culicidae).ultraviolet (uv) radiation is a key limiting factor for biological pest control with entomopathogenic fungi. while little is known about the impact of uv on metarhizium anisopliae metchnikoff (sorokin) (hypocreales: clavicipitaceae) conidia in aquatic mosquito-breeding sites, this study determined the effect of uv-b on the viability and virulence of m. anisopliae sensu lato (s.l.) strain ip 46 in the laboratory against aedes aegypti (l.) (diptera: culicidae) larvae. conidia were treated in cups ...201829750411
experimental zika virus infection in aedes aegypti: susceptibility, transmission & co-infection with dengue & chikungunya viruses.there are reports about the susceptibility of aedes mosquitoes to zikv from various countries, however, no such information is available from indian sub-continent, although, high level of group cross-reactivity of zikv with other flaviviruses has been reported. during outbreak situations, many cases of dengue (den) and chikungunya (chik) are reported. in such scenario, vector mosquitoes are likely to get co-infection/secondary-infection with one or other virus. the present study was carried out ...201829749366
a new "american" subgroup of african-lineage chikungunya virus detected in and isolated from mosquitoes collected in haiti, part of on-going arboviral surveillance activity in a semi-rural region in haiti, chikungunya virus (chikv)-positive mosquito pools were identified in 2014 (the peak of the caribbean asian-clade epidemic), and again in 2016 by rt-pcr. in 2014, chikv was only identified in aedes aegypti (11 positive pools/124 screened). in contrast, in sampling in 2016, chikv was not identified in ae. aegypti, but, rather, in (a) a female aedes albopictus pool, and (b) a female culex quinquefasciatus pool. gen ...201829746539
seasonal temperature variation influences climate suitability for dengue, chikungunya, and zika transmission.dengue, chikungunya, and zika virus epidemics transmitted by aedes aegypti mosquitoes have recently (re)emerged and spread throughout the americas, southeast asia, the pacific islands, and elsewhere. understanding how environmental conditions affect epidemic dynamics is critical for predicting and responding to the geographic and seasonal spread of disease. specifically, we lack a mechanistic understanding of how seasonal variation in temperature affects epidemic magnitude and duration. here, we ...201829746468
atypical neurological manifestations of chikungunya fever: two case reports.chikungunya fever (chik) is an arboviral disease which is transmitted by a mosquito bite (aedes aegypti). fever, polyarthralgia, and maculopapular rash are hallmark clinical manifestations of this disease. these manifestations are self-liming and complete recovery is seen in most of the cases. however, atypical and lethal complications such as myocarditis, hepatitis, guillain-barre syndrome (gbs), and meningoencephalitis have been reported in few cases of chik. we hereby report two cases of chik ...201829743772
complete genome of aedes aegypti anphevirus in the aag2 mosquito cell line.a novel negative-sense rna virus, aedes aegypti anphevirus, was recently identified in wild aedes aegypti mosquitoes. we show that this virus is also present in the aag2 aedes aegypti cell line and characterize its complete genome and evolutionary history. the aedes aegypti anphevirus genome is estimated to be 12 916 nucleotides in length, contains four genes and has a genome structure similar to that of other anpheviruses. phylogenetically, aedes aegypti anphevirus falls within an unclassified ...201829741476
expression of a synthetic gene for the major cytotoxin (cyt1aa) of bacillus thuringiensis subsp. israelensis in the chloroplast of wild-type chlamydomonas.chlamydomonas reinhardtii (chlamydomonas) strains that are toxic to mosquito larvae because they express chloroplast transgenes that are based on the mosquitocidal proteins of bacillus thuringiensis subsp. israelensis (bti) could be very useful in mosquito control. chlamydomonas has several advantages for this approach, including genetic controls not generally available with industrial algae. the bti toxin is produced by sporulating bacteria and has been used for mosquito control for >30 years w ...201829738473
crystal structure of a novel kunitz type inhibitor, alocasin with anti-aedes aegypti activity targeting midgut proteases.the pesticidal properties of many kunitz-type inhibitors have been reported previously; however, the mechanism of action is not well established. in this study, the activity of alocasin against aedes aegypti is demonstrated and the structure-activity relationship of this kunitz-type inhibitor is explained through x-ray structure analyses.201829737039
antibodies against chikungunya in northern mozambique during dengue outbreak, outbreak of dengue and high densities of aedes aegypti were reported in 2014 in northern mozambique, suggesting an increased risk for other arboviruses such as chikungunya virus (chikv) in this region. the aim of this study was to investigate the occurrence of chikv during an outbreak of dengue virus (denv) in pemba city in northern mozambique in 2014. febrile patients (n = 146) seeking medical attention at the pemba provincial hospital between march and april 2014 were enrolled in this study ...201829733254
male contributions during mating increase female survival in the disease vector mosquito aedes aegypti.aedes aegypti is a vector of medically important viruses including those causing zika, dengue, and chikungunya. during mating, males transfer a number of proteins and other molecules to the female and these components of the male ejaculate are essential in shifting female post-mating behaviors in a number of insect species. because these molecules are highly variable by species, and female post-mating behavior by species is also varied, behavioral assays testing the function of the ejaculate are ...201829729859
improving the delivery and efficiency of fungus-impregnated cloths for control of adult aedes aegypti using a synthetic attractive lure.entomopathogenic fungi are highly promising agents for controlling aedes aegypti mosquitoes. deploying fungus-impregnated black cloths in pet traps efficiently reduced ae. aegypti female survival rates under intra-domicile conditions. with the aim of further increasing the effectiveness of the traps, the addition of attractive lures to fungus-impregnated traps was evaluated.201829728121
advising a woman with suspected zika virus infection.the zika virus (zikv) epidemic is spreading: 67 countries are now reporting transmission, and over 2,000 cases of congenital zika syndrome (czs) have been confirmed. the heaviest burden has been borne by those living where poverty, poor infrastructure, and lack of access to health services are common and the penetration of aedes aegypti is high. because most cases are asymptomatic, the most dramatic signs of the disease appear through the czs cases. in spite of the need for disaggregated epidemi ...201829724698
larval density mediates knockdown resistance to pyrethroid insecticides in adult aedes aegypti.understanding mechanisms driving insecticide resistance in vector populations remains a public health priority. to date, most research has focused on the genetic mechanisms underpinning resistance, yet it is unclear what role environmental drivers may play in shaping phenotypic expression. one of the key environmental drivers of aedes aegypti mosquito population dynamics is resource-driven intraspecific competition at the larval stage. we experimentally investigated the role of density-dependent ...201829724237
contributions from the silent majority dominate dengue virus transmission.despite estimates that, each year, as many as 300 million dengue virus (denv) infections result in either no perceptible symptoms (asymptomatic) or symptoms that are sufficiently mild to go undetected by surveillance systems (inapparent), it has been assumed that these infections contribute little to onward transmission. however, recent blood-feeding experiments with aedes aegypti mosquitoes showed that people with asymptomatic and pre-symptomatic denv infections are capable of infecting mosquit ...201829723307
wolbachia significantly impacts the vector competence of aedes aegypti for mayaro virus.wolbachia, an intracellular endosymbiont present in up to 70% of all insect species, has been suggested as a sustainable strategy for the control of arboviruses such as dengue, zika and chikungunya. as mayaro virus outbreaks have also been reported in latin american countries, the objective of this study was to evaluate the vector competence of brazilian field-collected ae. aegypti and the impact of wolbachia (wmel strain) upon this virus. our in vitro studies with aag2 cells showed that mayaro ...201829720714
vector competence of certain culex and aedes mosquitoes for the chittoor virus, the indian variant of the batai virus.chittoor virus (chitv), a mosquito-borne bunyavirus (orthobunyavirus: bunyaviridae) isolated in india, has been found to be antigenically close to the batai virus (batv), which has a wide distribution across asia, europe, and africa. the latter virus causes influenza-like illness in humans and mild illness in sheep and goats. batv has been involved in genetic reassortment with other bunyaviruses, generating novel genome combinations and causing severe clinical manifestations including hemorrhagi ...201829718685
high throughput discovery of protein variants using proteomics informed by transcriptomics.proteomics informed by transcriptomics (pit), in which proteomic ms/ms spectra are searched against open reading frames derived from de novo assembled transcripts, can reveal previously unknown translated genomic elements (tges). however, determining which tges are truly novel, which are variants of known proteins, and which are simply artefacts of poor sequence assembly, is challenging. we have designed and implemented an automated solution that classifies putative tges by comparing to referenc ...201829718325
dehydration prompts increased activity and blood feeding by mosquitoes.current insights into the mosquito dehydration response rely on studies that examine specific responses but ultimately fail to provide an encompassing view of mosquito biology. here, we examined underlying changes in the biology of mosquitoes associated with dehydration. specifically, we show that dehydration increases blood feeding in the northern house mosquito, culex pipiens, which was the result of both higher activity and a greater tendency to land on a host. similar observations were noted ...201829717151
efficacy of larvicides for the control of dengue, zika, and chikungunya vectors in an urban cemetery in southern mexico.many countries in latin america have recently experienced outbreaks of zika and chikungunya fever, in additional to the usual burden imposed by dengue, all of which are transmitted by aedes aegypti in this region. to identify potential larvicides, we determined the toxicity of eight modern insecticides to a. aegypti larvae from a colony that originated from field-collected insects in southern mexico. the most toxic compounds were pyriproxyfen (which prevented adult emergence) and λ-cyhalothrin, ...201829713901
parallel evolution of vgsc mutations at domains is6, iis6 and iiis6 in pyrethroid resistant aedes aegypti from mexico.aedes aegypti is the primary urban mosquito vector of viruses causing dengue, zika and chikungunya fevers -for which vaccines and effective pharmaceuticals are still lacking. current strategies to suppress arbovirus outbreaks include removal of larval-breeding sites and insecticide treatment of larval and adult populations. insecticidal control of ae. aegypti is challenging, due to a recent rapid global increase in knockdown-resistance (kdr) to pyrethroid insecticides. widespread, heavy use of p ...201829712956
identification of candidate iron transporters from the zip/znt gene families in the mosquito aedes aegypti.mosquito-transmitted viral pathogens, such as dengue and zika, afflict tens of thousands of people every year. these viruses are transmitted during the blood-feeding process that is required for mosquito reproduction, the most important vector being aedes aegypti. while vertebrate blood is rich in protein, its high iron content is potentially toxic to mosquitoes. although iron transport and sequestration are essential in the reproduction of vector mosquitoes, we discovered that culicine mosquito ...201829706902
dengue fever in lebanon: first confirmed case since 1945 and review from the region.lebanon yearly witnesses a high flux of expatriates and workers from dengue virus (denv) endemic regions. multiple cases of dengue fever have been documented at the american university of beirut medical center (aubmc) in travellers to endemic regions. given the presence of the aedes aegypti mosquito in lebanon, introduction of denv to the country is highly likely. we report a case of denv infection in lebanon diagnosed in april 2012 in a patient with no prior travel history. the patient presente ...201831851640
[applications of the crispr/cas9 system in insects].the crispr/cas9 (clustered regularly interspaced short palindromic repeat/crispr-associated) system guides cas9 to specific genomic locations by a short rna search string. this technology enables the systematic interrogation of mammalian genome editing, repairing damaged genes, silencing harmful genes and improving quality traits. in recent years, with the introduction of the crispr/cas9 system for easy, fast and efficient genetic modification, it has been possible to conduct meaningful function ...201829704373
structural characterization of bacillus licheniformis dahb1 exopolysaccharide-antimicrobial potential and larvicidal activity on malaria and zika virus mosquito vectors.microbial polysaccharides produced by marine species play a key role in food and cosmetic industry, as they are nontoxic and biodegradable polymers. this investigation reports the isolation of exopolysaccharide from bacillus licheniformis dahb1 and its biomedical applications. bacillus licheniformis dahb1 exopolysaccharide (bl-eps) was extracted using the ethanol precipitation method and structurally characterized. ftir and 1h-nmr pointed out the presence of various functional groups and primary ...201829704178
host genotype and environment affect the trade-off between horizontal and vertical transmission of the parasite edhazardia aedis.if a parasite is able to transmit horizontally or vertically, which transmission mode will it choose? we investigated how the growth conditions and the genotype of the mosquito aedes aegypti affect the transmission mode of the parasite edhazardia aedis.201829699504
ammonia excretion in an osmoregulatory syncytium is facilitated by aeamt2, a novel ammonia transporter in aedes aegypti larvae.the larvae of the mosquito aedes aegypti inhabit ammonia rich septic tanks in tropical regions of the world that make extensive use of these systems, explaining the prevalence of disease during dry seasons. since ammonia (nh3/[formula: see text]) is toxic to animals, an understanding of the physiological mechanisms of ammonia excretion permitting the survival of a. aegypti larvae in high ammonia environments is important. we have characterized a novel ammonia transporter, aeamt2, belonging to th ...201829695971
a chikungunya virus trans-replicase system reveals the importance of delayed nonstructural polyprotein processing for efficient replication complex formation in mosquito cells.chikungunya virus (chikv) is a medically important alphavirus that is transmitted by aedes aegypti and aedes albopictus mosquitoes. the viral replicase complex consists of four nonstructural proteins (nsps) expressed as a polyprotein precursor and encompasses all enzymatic activities required for viral rna replication. nsps interact with host components of which most are still poorly understood, especially in mosquitos. a chikv trans-replicase system that allows the uncoupling of rna replication ...201829695432
effects of desiccation stress on adult female longevity in aedes aegypti and ae. albopictus (diptera: culicidae): results of a systematic review and pooled survival analysis.transmission dynamics of mosquito-borne viruses such as dengue, zika and chikungunya are affected by the longevity of the adult female mosquito. environmental conditions influence the survival of adult female aedes mosquitoes, the primary vectors of these viruses. while the association of temperature with aedes mortality has been relatively well-explored, the role of humidity is less established. the current study's goals were to compile knowledge of the influence of humidity on adult survival i ...201829695282
dna barcoding of morphologically characterized mosquitoes belonging to the subfamily culicinae from sri lanka.vectors of mosquito-borne diseases in sri lanka, except for malaria, belong to the subfamily culicinae, which includes nearly 84% of the mosquito fauna of the country. hence, accurate and precise species identification of culicine mosquitoes is a crucial factor in implementing effective vector control strategies. during the present study, a combined effort using morphology and dna barcoding was made to characterize mosquitoes of the subfamily culicinae for the first time from nine districts of s ...201829695263
characterization of a western pacific zika virus strain in australian aedes aegypti.zika virus (zikv) is a globally emerging arbovirus responsible for widespread epidemics in the western pacific, the americas, and asia. the virus predominately circulates in urban transmission cycles between aedes aegypti and humans. australia is considered at risk to outbreaks of zikv due to the presence of a. aegypti populations in northern areas of the state of queensland. furthermore, close proximity to epidemic regions has led to almost 50% of imported cases reported since 2012 originating ...201829694294
neurotropism and behavioral changes associated with zika infection in the vector aedes aegypti.understanding zika virus infection dynamics is essential, as its recent emergence revealed possible devastating neuropathologies in humans, thus causing a major threat to public health worldwide. recent research allowed breakthrough in our understanding of the virus and host pathogenesis; however, little is known on its impact on its main vector, aedes aegypti. here we show how zika virus targets aedes aegypti's neurons and induces changes in its behavior. results are compared to dengue virus, a ...201829691362
dengue virus infection induces chromatin remodeling at locus aael006536 in the midgut of aedes identify and characterize aedes aegypti's aael006536 gene proximal upstream cis-regulatory sequences activated by dengue virus infection.201829689655
association of dengue fever with aedes spp. abundance and climatological analyze the association of dengue fever incidence with aedes mosquito's abundance, and the effect of climatological and geographical variables, in a region in morelos state, mexico.201829689652
the redox-sensing gene nrf2 affects intestinal homeostasis, insecticide resistance, and zika virus susceptibility in the mosquito aedes aegypti.production and degradation of reactive oxygen species (ros) are extensively regulated to ensure proper cellular responses to various environmental stimuli and stresses. moreover, physiologically generated ros function as secondary messengers that can influence tissue homeostasis. the cap'n'collar transcription factor known as nuclear factor erythroid-derived factor 2 (nrf2) coordinates an evolutionarily conserved transcriptional activation pathway that mediates antioxidant and detoxification res ...201829685890
dispersion and oviposition of aedes albopictus in a brazilian slum: initial evidence of asian tiger mosquito domiciliation in urban environments.aedes albopictus, originally considered as a secondary vector for arbovirus transmission, especially in areas where this species co-exist with aedes aegypti, has been described in most regions of the world. dispersion and domiciliation of ae. albopictus in a complex of densely urbanized slums in rio de janeiro, southeastern brazil, was evidenced. in this study, we tested the hypotheses that 1) ae. albopictus distribution in urban slums is negatively related to distance from vegetation, and 2) th ...201829684029
entomopathogenic fungal infection leads to temporospatial modulation of the mosquito immune system.alternative methods of mosquito control are needed to tackle the rising burden of mosquito-borne diseases while minimizing the use of synthetic insecticides, which are threatened by the rapid increase in insecticide resistance in mosquito populations. fungal biopesticides show great promise as potential alternatives because of their ecofriendly nature and ability to infect mosquitoes on contact. here we describe the temporospatial interactions between the mosquito aedes aegypti and several entom ...201829684026
dengue fever and aedes aegypti in indigenous brazilians: seroprevalence, risk factors, knowledge and practices.dengue remains an important public health problem in brazil. we estimated the associated factors of dengue seroprevalence among native indians of the tremembé ethnic and their knowledge about the aspects related to the presence of mosquitoes of the genus aedes.201829673030
susceptibility status and resistance mechanisms in permethrin-selected, laboratory susceptible and field-collected aedes aegypti from malaysia.this study is intended to provide a comprehensive characterization of the resistance mechanisms in the permethrin-selected (imr-pss) and laboratory susceptible (imr-ls) aedes aegypti strain from malaysia. both imr-pss and imr-ls provide a standard model for use in assessing the pyrethroid resistance in field-collected strains collected from three dengue hotspots: the taman seri bayu (tsb), the flat camar (fc), and the taman dahlia (td). two established methods for determining the resistance mech ...201829670071
locomotor activity in males of aedes aegypti can shift in response to females' presence.the study of physiological and behavioral traits of mosquito vectors has been of growing relevance for the proposition of alternative methods for controlling vector-borne diseases. despite this, most studies focus on the female's traits, including the behavior of host seeking, the physiology of disease transmission and the site-choice for oviposition. however, understanding the factors that lead to males' reproductive success is of utmost importance, since it can help building new strategies for ...201829669591
fly ash-based water dispersible powder formulation of bacillus thuringiensis var. israelensis: development & laboratory evaluation against mosquito immatures.bacillus thuringiensis var. israelensis (bti) formulations are presently being used for insect control. in this study, a water dispersible powder (wdp) formulation using fly ash (fa) as a carrier material was developed and studied for its activity against the larval stages of major mosquito vector species.201729664029
variation in wolbachia effects on aedes mosquitoes as a determinant of invasiveness and vectorial capacity.wolbachia has been introduced into aedes aegypti mosquitoes to control the spread of arboviruses, such as dengue, chikungunya and zika. studies showed that certain wolbachia strains (such as wmel) reduce replication of dengue viruses in the laboratory, prompting the release of mosquitoes carrying the bacterium into the field, where vectorial capacity can be realistically assessed in relation to native non-carriers. here we apply a new analysis to two published datasets, and show that wmel increa ...201829662096
numerical modeling of mosquito population dynamics of aedes aegypti.the global incidences of dengue virus have increased the interest in studying and understanding the mosquito population dynamics. it is predominantly spread by aedes aegypti in the tropical and sub-tropical countries in the world. understanding these dynamics is important for public health in countries where climatic and environmental conditions are favorable for the propagation of these diseases. for this reason, a new model has been proposed to investigate the population dynamics of mosquitoes ...201829661212
factors related to aedes aegypti (diptera: culicidae) populations and temperature determine differences on life-history traits with regional implications in disease transmission.aedes aegypti (l.) (diptera: culicidae) is a vector of many medically significant viruses in the americas, including dengue virus, chikungunya virus, and zika virus. traits such as longevity, fecundity, and feeding behavior contribute to the ability of ae. aegypti to serve as a vector of these pathogens. both local environmental factors and population genetics could contribute to variability in these traits. we performed a comparative study of ae. aegypti populations from four geographically and ...201829659944
detection of aedes (stegomyia) aegypti (diptera: culicidae) populations in southern alabama following a 26-yr absence and public perceptions of the threat of zika virus.with the establishment of zika virus in the americas, an accurate understanding of the geographic range of its primary vector, aedes (stegomyia) aegypti (l.) (diptera: culicidae), is vital to assessing transmission risk. in an article published in june 2016, hahn and colleagues compiled county-level records in the united states for the presence of ae. aegypti and aedes (stegomyia) albopictus (skuse) (diptera: culicidae) reported between january 1995 and march 2016. despite ecological suitability ...201829659928
coinfection with zika virus (zikv) and dengue virus results in preferential zikv transmission by vector bite to vertebrate host.several tropical cities are permissive to aedes aegypti and dengue virus (denv) endemicity and have allowed for invasion and circulation of zika virus (zikv) in the same areas. people living in arbovirus-endemic regions have been simultaneously infected with ≥2 arboviruses.201829659904
ultrasound is not useful as a screening tool for dengue fever.dengue fever is a tropical disease that is transmitted by female aedes aegypti mosquitos. early diagnosis is necessary to reduce the mortality and morbidity associated with the disease. a combination of clinical, laboratory, and sonography findings can be potentially helpful in making an early diagnosis of dengue fever. there is paucity of literature on the use of ultrasound for dengue fever screening; hence, the primary objective of the study was to evaluate the utility of ultrasound as a scree ...201729657635
co-breeding association of aedes albopictus (skuse) and aedes aegypti (linnaeus) (diptera: culicidae) in relation to location and container size.the occurrence of major outbreaks of dengue, and other vector borne diseases such as chikungunya and zika in tropical and subtropical regions has rendered control of the diseases a top-priority for many affected countries including malaysia. control of the mosquito vectors aedes aegypti and aedes albopictus through the reduction of breeding sites and the application of insecticides to kill immature forms and adults are the main control efforts to combat these diseases. the present study describe ...201829644025
evaluation of a simple polytetrafluoroethylene (ptfe)-based membrane for blood-feeding of malaria and dengue fever vectors in the laboratory.controlled blood-feeding is essential for maintaining laboratory colonies of disease-transmitting mosquitoes and investigating pathogen transmission. we evaluated a low-cost artificial feeding (af) method, as an alternative to direct human feeding (dhf), commonly used in mosquito laboratories.201829642937
yellow fever.the yellow fever (yf) virus is a flavivirus, transmitted by haemagogus, sabethes or aedes aegypti mosquitoes. the disease is endemic in forest areas in africa and latin america leading to epizootics in monkeys that constitute the reservoir of the disease. there are two forms of yf: sylvatic, transmitted accidentally when approaching the forests, and urban, which can be perpetuated by aedes aegypti. in brazil, the last case of urban yf occurred in 1942. since then, there has been an expansion of ...201829641667
identification of entomopathogenic nematodes and symbiotic bacteria from nam nao national park in thailand and larvicidal activity of symbiotic bacteria against aedes aegypti and aedes albopictus.entomopathogenic nematodes (epns) that are symbiotically associated with xenorhabdus and photorhabdus bacteria can kill target insects via direct infection and toxin action. there are limited reports identifying such organisms in the national park of thailand. therefore, the objectives of this study were to identify epns and symbiotic bacteria from nam nao national park, phetchabun province, thailand and to evaluate the larvicidal activity of bacteria against aedes aegypti and ae. albopictus. a ...201829641570
suppression of the pelo protein by wolbachia and its effect on dengue virus in aedes aegypti.the endosymbiont wolbachia is known to block replication of several important arboviruses, including dengue virus (denv), in the mosquito vector aedes aegypti. so far, the exact mechanism of this viral inhibition is not fully understood. a recent study in drosophila melanogaster has demonstrated an interaction between the pelo gene and drosophila c virus. in this study, we explored the possible involvement of the pelo protein, that is involved in protein translation, in wolbachia-mediated antivi ...201829641562
exploring mosquito fauna of majuro atoll (republic of marshall islands) in the context of zika outbreak.first autochthonous zika clinical case was reported in the republic of marshall islands (rmi) on majuro atoll in february 2016. an entomological survey of mosquito larvae and adult populations was carried out in four areas of majuro, the most populated atoll of rmi encompassing different habitats (forest, rural, or urban) including some with confirmed clinical zika cases to evaluate which mosquito species could be involved in the zika transmission. a total of 2,367 immature and adult mosquito sp ...201829635382
a sex-structured model with birth pulse and release strategy for the spread of wolbachia in mosquito population.dengue fever is one of the most important diseases causing illness and death all over the world, which brings tremendous threat to peoples' life and property security, especially in the undeveloped areas. the main vector, aedes aegypti, must be controlled to prevent the transmission of dengue. there are a variety of methods to control it. wolbachia is an innovative bacterium which breaks the dengue transmission cycle for its characteristics of cytoplasmic incompatibility and maternal transmissio ...201829625205
efficacy of aedes aegypti control by indoor ultra low volume (ulv) insecticide spraying in iquitos, peru.aedes aegypti is a primary vector of dengue, chikungunya, zika, and urban yellow fever viruses. indoor, ultra low volume (ulv) space spraying with pyrethroid insecticides is the main approach used for ae. aegypti emergency control in many countries. given the widespread use of this method, the lack of large-scale experiments or detailed evaluations of municipal spray programs is problematic.201829624581
overview of dengue and zika virus similarity, what can we learn from the saudi experience with dengue fever?there is high public health alert in the kingdom of saudi arabia concerning zika virus infection. so far, there is no reported outbreak. so are we at risk of this disease? reviewing the literature of recent outbreaks of other infectious diseases in saudi arabia may clarify the situation. it is evident that there is some similarity between zika and dengue regarding vector (aedes aegypti) which is available in the kingdom of saudi arabia. furthermore, they have similar transmission process and the ...201929623022
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