response to comments on qureshi et al (j pak med assoc 2017; 67: 1797-1802) seasonal and spatial quantitative changes in aedes aegypti under distinctly different ecological areas of lahore, pakistan. 201830323379
comments on qureshi et al (j pak med assoc 2017; 67: 1797-1802) - seasonal and spatial quantitative changes in aedes aegypti under distinctly different ecological areas of lahore, pakistan. 201830323378
location, seasonal, and functional characteristics of water holding containers with juvenile and pupal aedes aegypti in southern taiwan: a cross-sectional study using hurdle model analyses.aedes aegypti carries several viruses of public health importance, including the dengue virus. dengue is the most rapidly spreading mosquito-borne viral disease in the world. prevention and control of dengue mainly rely on mosquito control as there is no antiviral treatment or a who-approved vaccine. to reduce the ae. aegypti population, studying the characteristics of their habitats is necessary. aedes aegypti prefer breeding in artificial water holding containers in peridomestic or domestic se ...201830321168
effects of alternative blood sources on wolbachia infected aedes aegypti females within and across generations.wolbachia bacteria have been identified as a tool for reducing the transmission of arboviruses transmitted by aedes aegypti. research groups around the world are now mass rearing wolbachia-infected ae. aegypti for deliberate release. we investigated the fitness impact of a crucial element of mass rearing: the blood meal required by female ae. aegypti to lay eggs. although ae. aegypti almost exclusively feed on human blood, it is often difficult to use human blood in disease-endemic settings. whe ...201830314399
genetic variation in insect vectors: death of typology?the issue of typological versus population thinking in biology is briefly introduced and defined. it is then emphasized how population thinking is most relevant and useful in vector biology. three points are made: (1) vectors, as they exist in nature, are genetically very heterogeneous. (2) four examples of how this is relevant in vector biology research are presented: understanding variation in vector competence, gwas, identifying the origin of new introductions of invasive species, and resista ...201830314367
zika virus: a critical analysis and pharmaceutical perspectives.zika virus belongs to family of viruses 'flaviviridae' and spreads mostly by daytime-active aedes mosquitoes. the resulting infection is known as zika fever. it is usually asymptomatic or often causes mild symptoms, which are very similar to dengue fever. the aedes aegypti mosquito is also responsible for dengue and chikungunya viruses. zika virus can spread by crossing the placental barrier from a pregnant mother to a fetus, which can result in microcephaly, severe brain malformations including ...201830311585
human and entomologic investigations of chikungunya outbreak in mandera, northeastern kenya, 2016.chikungunya is a reemerging vector borne pathogen associated with severe morbidity in affected populations. lamu, along the kenyan coast was affected by a major chikungunya outbreak in 2004. twelve years later, we report on entomologic investigations and laboratory confirmed chikungunya cases in northeastern kenya. patient blood samples were received at the kenya medical research institute (kemri) viral hemorrhagic fever laboratory and the immunoglobulin m enzyme linked immunosorbent assay (igm ...201830308064
spatio-temporal distribution of vertically transmitted dengue viruses by aedes aegypti (diptera: culicidae) from arroyo naranjo, havana, study the distribution of vertical transmission of dengue viruses in field-collected aedes aegypti larvae in the municipality of arroyo naranjo in havana, cuba.201830307674
evidence that rh proteins in the anal papillae of the freshwater mosquito aedes aegypti are involved in the regulation of acid-base balance in elevated salt and ammonia environments.aedes aegypti commonly inhabit ammonia-rich sewage effluents in tropical regions of the world where the adults are responsible for the spread of disease. studies have shown the importance of the anal papillae of a. aegypti in ion uptake and ammonia excretion. the anal papillae express ammonia transporters and rhesus (rh) proteins which are involved in ammonia excretion and studies have primarily focused on understanding these mechanisms in freshwater. in this study, effects of rearing larvae in ...201830305376
the effect of parasite infection on the recombination rate of the mosquito aedes aegypti.sexual reproduction and meiotic recombination generate new genetic combinations and may thereby help an individual infected by a parasite to protect its offspring from being infected. while this idea is often used to understand the evolutionary forces underlying the maintenance of sex and recombination, it also suggests that infected individuals should increase plastically their rate of recombination. we tested the latter idea with the mosquito aedes aegypti and asked whether females infected by ...201830300349
vector competence analysis of two aedes aegypti lineages from bello, colombia, reveals that they are affected similarly by dengue-2 virus infection.dengue is the second most prevalent vector-borne disease after malaria in colombia. it is caused by dengue virus, an arbovirus that exhibits high epidemic power, which is evidenced by its occurrence in more than 80% of the country, largely because of the extensive dispersion of the mosquito vector aedes aegypti. the existence of two lineages of ae. aegypti has been proposed based on genetic differences at the mitochondrial level, and they have been reported to circulate in similar proportions in ...201930298277
neuronal responses of antennal olfactory sensilla to insect chemical repellents in the yellow fever mosquito, aedes aegypti.the yellow fever mosquito, aedes aegypti, is a vector of many human diseases such as yellow fever, dengue fever, and zika. as insecticide resistance has been widely reported, chemical repellents have been adopted as alternative options for mosquito and mosquito-borne disease control. this study characterized the responses of olfactory receptor neurons (orns) in different types of antennal olfactory sensilla in ae. aegypti to 48 chemicals that exhibited repellent activity in various insect specie ...201830291492
plant essential oils enhance diverse pyrethroids against multiple strains of mosquitoes and inhibit detoxification enzyme processes.mosquito-borne diseases account for the deaths of approximately 700,000 people annually throughout the world, with many more succumbing to the debilitating side effects associated with these etiologic disease agents. this is exacerbated in many countries where the lack of mosquito control and resources to prevent and treat mosquito-borne disease coincide. as populations of mosquito species grow more resistant to currently utilized control chemistries, the need for new and effective chemical mean ...201830287743
enhanced mortality in deltamethrin-resistant aedes aegypti in thailand using a piperonyl butoxide synergist.aedes aegypti is the primary vector of dengue viruses in thailand. control of this mosquito continues to rely heavily on use of insecticides in various forms and applications. the synergistic effect of piperonyl butoxide (pbo), combined with deltamethrin against eight populations of ae. aegypti collected from different regions in thailand is presented. the standard who adult contact bioassays found all populations with low to moderate levels of resistance to deltamethrin alone (using a 0.05% dis ...201930287252
projecting the end of the zika virus epidemic in latin america: a modelling analysis.zika virus (zikv) emerged in latin america and the caribbean (lac) region in 2013, with serious implications for population health in the region. in 2016, the world health organization declared the zikv outbreak a public health emergency of international concern following a cluster of associated neurological disorders and neonatal malformations. in 2017, zika cases declined, but future incidence in lac remains uncertain due to gaps in our understanding, considerable variation in surveillance and ...201830285863
relationship between local presence and density of aedes aegypti eggs with dengue cases: a spatial analysis analyze the relationship between the occurrence of dengue and ovitrap positivity and dengue egg density in two amazonas municipalities (brazil) in 2016.201830282110
seasonal and spatial distribution of aedes aegypti and aedes albopictus in a municipal urban park in são paulo, sp, brazil.dengue, yellow fever, chikungunya and zika are important arboviruses present in various countries of the world, the etiological agents of which are transmitted to human-beings by the bite of infected females of aedes aegypti and aedes albopictus. biological aspects of these vectors, such as their distribution and abundance, are influenced by climatic variables such as rainfall and temperature. we assess the spatial and seasonal distribution of ae. aegypti and ae. albopictus, during spring 2014 a ...201930282003
microcystin and pyriproxyfen are toxic to early stages of development in rhamdia quelen: an experimental and modelling study.the recent increase of freshwater eutrophication has favored cyanobacteria blooms and consequently the increase of toxins such as microcystin-lr in aquatic environments, but few is know about the associated effect of toxin and other compounds. pyriproxyfen is an insecticide indicated by who (world health organization) to control aedes aegypti mosquito (vector of dengue, chikungunya and zika diseases), however, the effects are not well described to non-target species, such as fish. the early life ...201830278392
serotonin signaling regulates insulin-like peptides for growth, reproduction, and metabolism in the disease vector aedes aegypti.disease-transmitting female mosquitoes require a vertebrate blood meal to produce their eggs. an obligatory hematophagous lifestyle, rapid reproduction, and existence of a large number of transmittable diseases make mosquitoes the world's deadliest animals. attaining optimal body size and nutritional status is critical for mosquitoes to become reproductively competent and effective disease vectors. we report that blood feeding boosts serotonin concentration and elevates the serotonin receptor aa ...201830275337
from incriminating stegomyia fasciata to releasing wolbachia pipientis: australian research on the dengue virus vector, aedes aegypti, and development of novel strategies for its surveillance and control.globally, the dengue viruses (denvs) infect approximately 300 million people annually. australia has a history of epidemic dengue, with outbreaks in the early decades of the twentieth century responsible for tens of thousands of cases. seminal experiments conducted by australian scientists during these outbreaks were the first to incriminate aedes aegypti as a major vector of dengue viruses. one hundred years later, australian scientists are playing a lead role in the development of surveillance ...201830274467
the importance of water typologies in lay entomologies of aedes aegypti habitat, breeding and dengue risk: a study from northern australia.dengue fever is making a significant comeback globally and its control still depends largely on residents' actions. community awareness and education are central to its management; however, programmes have had limited impact, because they are often based on short-term research and limited awareness of the socio-ecological contexts wherein local knowledge of dengue and its vectors (lay entomology) is produced and enacted in and through place. long-term studies of lay knowledge of dengue vectors a ...201830274463
application of artificial neural networks for dengue fever outbreak predictions in the northwest coast of yucatan, mexico and san juan, puerto rico.modelling dengue fever in endemic areas is important to mitigate and improve vector-borne disease control to reduce outbreaks. this study applied artificial neural networks (anns) to predict dengue fever outbreak occurrences in san juan, puerto rico (usa), and in several coastal municipalities of the state of yucatan, mexico, based on specific thresholds. the models were trained with 19 years of dengue fever data for puerto rico and six years for mexico. environmental and demographic data includ ...201830274404
synthetic mirnas induce dual arboviral-resistance phenotypes in the vector mosquito aedes aegypti.mosquito-borne arboviruses are responsible for recent dengue, chikungunya, and zika pandemics. the yellow-fever mosquito, aedes aegypti, plays an important role in the transmission of all three viruses. we developed a mirna-based approach that results in a dual resistance phenotype in mosquitoes to dengue serotype 3 (denv-3) and chikungunya (chikv) viruses. the target viruses are from two distinct arboviral families and the antiviral mechanism is designed to function through the endogenous mirna ...201830271898
hypometabolic strategy and glucose metabolism maintenance of aedes aegypti egg desiccation.the mosquito aedes aegypti is vector of several viruses including yellow fever virus, dengue virus chikungunya virus and zika virus. one of the major problems involving these diseases transmission is that a. aegypti embryos are resistant to desiccation at the end of embryogenesis, surviving and remaining viable for several months inside the egg. therefore, a fine metabolism control is essential to support these organisms throughout this period of resistance. the carbohydrate metabolism has been ...201930266630
aedes aegypti mosquitoes from guadeloupe (french west indies) are able to transmit yellow fever virus.the recent yellow fever epidemic in brazil has raised the concern of outbreaks in neighboring countries, particularly in the caribbean region where the vector aedes aegypti is predominant. this threat comes from the past when in the americas, this disease caused devastating urban epidemics. we report the vector competence of ae. aegypti from guadeloupe for yellow fever virus by determining different parameters describing virus infection, dissemination, and transmission. the results indicate that ...201830265716
efficacy of mosquito coils: cross-resistance to pyrethroids in aedes aegypti (diptera: culicidae) from indonesia.aedes aegypti (l.) (diptera: culicidae) is the primary vector of several arthropod-borne viral infectious diseases globally. relentless vector control efforts are performed to curtail disease transmissions, insecticides remain as the first line of defense in indonesia. with a dearth of publication on the efficacy of mosquito coil in indonesia, this is the first report related to mosquito coil despite its common use in households. ae. aegypti mosquitoes were sampled from nine regencies in indones ...201830265353
nanosilver crystals capped with bauhinia acuminata phytochemicals as new antimicrobials and mosquito develop novel nanoformulated insecticides and antimicrobials, herein we produced ag nanoparticles (agnps) using the bauhinia acuminata leaf extract. this unexpensive aqueous extract acted as a capping and reducing agent for the formation of agnps. we characterized b. acuminata-synthesized agnps by uv-vis and ftir spectroscopy, xrd and tem analyses. uv-vis spectroscopy analysis of b. acuminata-synthesized agnps showed a peak at 441.5 nm. ftir shed light on functional groups from the phytoconst ...201830262272
high functional conservation of takeout family members in a courtship model system.takeout (to) is one of the male-specific genes expressed in the fat body that regulate male courtship behavior, and has been shown to act as a secreted protein in conjunction with courtship circuits. there are 23 takeout family members in drosophila melanogaster, and homologues of this family are distributed across insect species. sequence conservation among family members is low. here we test the functional conservation of takeout family members by examining whether they can rescue the takeout ...201830261021
history of zikv infections in india and management of disease outbreaks.zika virus (zikv) is an emerging arbovirus infection endemic in multiple countries spread from asia, africa to the americas and europe. previously known to cause rare and fairly benign human infections, zikv has become a major international public health emergency after being linked to unexpected neurological complications, that includes fetal brain damage/death and microcephaly in babies born to infected mothers and guillain-barre syndrome (gbs) in adults. it appears that a single genetic mutat ...201830258421
first report of v1016g and s989p knockdown resistant (kdr) mutations in pyrethroid-resistant sri lankan aedes aegypti mosquitoes.dengue is a serious arboviral disease in sri lanka with a large number of dengue fever (df) cases every year. control of the primary vector aedes aegypti depends upon larval habitat source reduction and insecticide application. however, increases in the number of reported cases suggest the inefficiency of current control strategies and the possibility of resistance to currently used insecticides. early detection of mutations in the voltage-gated sodium channel (vgsc) gene that confer knockdown r ...201830257701
c-type lectin-20 interacts with alp1 receptor to reduce cry toxicity in aedes aegypti.aedes aegypti is a crucial vector for human diseases, such as yellow fever, dengue, chikungunya, and zika viruses. today, a major challenge throughout the globe is the insufficient availability of antiviral drugs and vaccines against arboviruses, and toxins produced by bacillus thuringiensis (bt) are still used as biological agents for mosquito control. the use of cry toxins to kill insects mainly depends on the interaction between cry toxins and important toxin receptors, such as alkaline phosp ...201830257487
differential transmission of asian and african zika virus lineages by aedes aegypti from new caledonia.zika virus (zikv) is a flavivirus that is transmitted to humans by aedes mosquitoes. zikv is divided into two phylogenetic lineages, african and asian. in the asian lineage, pacific and american clades have been linked to the recent worldwide outbreak of zikv. the aim of this study was to measure the vector competence of aedes aegypti for seven zikv strains belonging to both lineages. we demonstrate that ae. aegypti from new caledonia (nc), south pacific region, is a low-competence vector for as ...201830254274
sex and species specific hearing mechanisms in mosquito flagellar ears.hearing is essential for the courtship of one of the major carriers of human disease, the mosquito. males locate females through flight-tone recognition and both sexes engage in mid-air acoustic communications, which can take place within swarms containing thousands of individuals. despite the importance of hearing for mosquitoes, its mechanisms are still largely unclear. we here report a multilevel analysis of auditory function across three disease-transmitting mosquitoes (aedes aegypti, anophe ...201830254270
population structure of a vector of human diseases: aedes aegypti in its ancestral range, africa.aedes aegypti, the major vector of dengue, yellow fever, chikungunya, and zika viruses, remains of great medical and public health concern. there is little doubt that the ancestral home of the species is africa. this mosquito invaded the new world 400-500 years ago and later, asia. however, little is known about the genetic structure and history of ae. aegypti across africa, as well as the possible origin(s) of the new world invasion. here, we use ~17,000 genome-wide single nucleotide polymorphi ...201830250667
microbiome interaction networks and community structure from laboratory-reared and field-collected aedes aegypti, aedes albopictus, and culex quinquefasciatus mosquito vectors.microbial interactions are an underappreciated force in shaping insect microbiome communities. although pairwise patterns of symbiont interactions have been identified, we have a poor understanding regarding the scale and the nature of co-occurrence and co-exclusion interactions within the microbiome. to characterize these patterns in mosquitoes, we sequenced the bacterial microbiome of aedes aegypti, ae. albopictus, and culex quinquefasciatus caught in the field or reared in the laboratory and ...201830250462
the intrinsically disordered c-terminal f domain of the ecdysteroid receptor from aedes aegypti exhibits metal ion-binding ability.the dominant vector of dengue and zika diseases is a female aedes aegypti mosquito. its reproduction is controlled by the formation of an active heterodimer complex of the 20-hydroxyecdysone receptor (ecr) and ultraspiracle protein (usp). although ecr exhibits a structural and functional organization typical of nuclear receptors (nrs), the ecr c-terminus has an additional f domain (aafecr) that is rarely present in the nrs superfamily. the presence of f domains is evolutionarily not well conserv ...201930243841
enhancement of insect susceptibility and larvicidal efficacy of cry4ba toxin by calcofluor.mosquitoes transmit many vector-borne infectious diseases including malaria, dengue, chikungunya, yellow fever, filariasis, and japanese encephalitis. the insecticidal δ-endotoxins cry4, cry11, and cyt produced from bacillus thuringiensis have been used for bio-control of mosquito larvae. cry δ-endotoxins are synthesised as inactive protoxins in the form of crystalline inclusions in which they are processed to active toxins in larval midgut lumen. previously, we demonstrated that the activated c ...201830236155
determining environmental and anthropogenic factors which explain the global distribution of aedes aegypti and ae. albopictus.responsible for considerable global human morbidity and mortality, aedes aegypti and ae. albopictus are the primary vectors of several important human diseases, including dengue and yellow fever. although numerous variables that affect mosquito survival and reproduction have been recorded at the local and regional scales, many remain untested at the global level, potentially confounding mapping efforts to date.201830233829
evaluation of effectiveness of a community-based intervention for control of dengue virus vector, ouagadougou, burkina faso.we evaluated the effectiveness of a community-based intervention for dengue vector control in ouagadougou, the capital city of burkina faso. households in the intervention (n = 287) and control (n = 289) neighborhoods were randomly sampled and the outcomes collected before the intervention (october 2015) and after the intervention (october 2016). the intervention reduced residents' exposure to dengue vector bites (vector saliva biomarker difference -0.08 [95% ci -0.11 to -0.04]). the pupae index ...201830226159
baseline characterization of dengue epidemiology in yogyakarta city, indonesia, before a randomized controlled trial of wolbachia for arboviral disease control.dengue is endemic in indonesia. here, we describe the epidemiology of dengue in the city of yogyakarta, central java, as a prelude to implementation of a cluster-randomized trial of wolbachia for the biocontrol of arboviral transmission. surveillance records from 2006 to 2016 demonstrate seasonal oscillations of dengue incidence with varying magnitude. two lines of evidence demonstrate a high force of infection; the hospitalized case burden of patients diagnosed with dengue hemorrhagic fever or ...201830226138
a comparison of mosquito densities, weather and infection rates of aedes aegypti during the first epidemics of chikungunya (2014) and zika (2016) in areas with and without vector control in puerto puerto rico, the first records of the transmission of chikungunya (chikv) and zika (zikv) viruses were confirmed in may 2014 and december 2015, respectively. transmission of chikv peaked in september 2014, whereas that of zikv peaked in august 2016. the emergence of these mosquito-transmitted arboviruses in the context of a lack of human population immunity allowed observations of whether the outbreaks were associated with aedes aegypti (diptera: culicidae) densities and weather. mosquito den ...201930225842
the surveillance of chikungunya virus in a temperate climate: challenges and possible solutions from the experience of lazio region, italy.chikv has become an emerging public health concern in the temperate regions of the northern hemisphere as a consequenceof the expansion of the endemic areas of its vectors (mainly aedes aegypti and aedesalbopictus). in 2017, a new outbreak of chikv was detected in italy with three clusters of autochthonous transmission in the lazio region (central italy), in the cities of anzio, rome, and latina and a secondary cluster in the calabria region (south italy). given the climate characteristics of it ...201830223536
where vectors collide: the importance of mechanisms shaping the realized niche for modeling ranges of invasive aedes mosquitoes.the vector mosquitoes aedes aegypti (l.), native to africa, and aedes albopictus (skuse), native to asia, are widespread invasives whose spatial distributions frequently overlap. predictive models of their distributions are typically correlative rather than mechanistic, and based on only abiotic variables describing putative environmental requirements despite extensive evidence of competitive interactions leading to displacements. here we review putative roles of competition contributing to dist ...201830220875
vaccination and immunization strategies to design aedes aegypti salivary protein based subunit vaccine tackling flavivirus infection.flavivirus causes arthropod-borne severe diseases that sometimes lead to the death. the flavivirus species including dengue virus, zika virus and yellow fever virus are transmitted by the bite of aedes mosquitoes. all these viral species target the people living in their respective endemic zone causing a high mortality rate. recent studies show that immune factors present in the ae. aegypti saliva is the hidden culprit promoting blood meal collection, suppressing host immune molecules and promot ...201930219509
potential of aedes aegypti populations in madeira island to transmit dengue and chikungunya viruses.aedes spp. mosquitoes mainly transmit the arboviruses dengue virus (denv) and chikungunya virus (chikv) in urban areas, causing a severe public health problem. in 2012-2013, a major dengue outbreak occurred on madeira island where the mosquito aedes aegypti was the only vector. up to now, the competence of ae. aegypti populations from madeira to transmit denv or chikv remains unknown. this study aimed to assess experimentally the ability of ae. aegypti populations from madeira to transmit these ...201830208974
distribution and breeding sites of aedes aegypti and aedes albopictus in 32 urban/peri-urban districts of mozambique: implication for assessing the risk of arbovirus outbreaks.aedes-borne arboviruses have emerged as an important public health problem worldwide and, in mozambique, the number of cases and its geographical spread have been growing. however, information on the occurrence, distribution and ecology of aedes aegypti and ae. albopictus mosquitoes remain poorly known in the country.201830208017
mosquito and tick repellency of two anthemis essential oils from saudi arabia.the essential oils (eos) of anthemis melampodina (am) and anthemis scrobicularis (as) (asteraceae) were extracted from the aerial parts of the plants by hydrodistillation, and their chemical compositions were analyzed using gc-fid and gc-ms. fifty-six components representing 85.5% of the oil composition of anthemis melampdina were identified, and the major components were α-pinene (17.1%) and β-eudesmol (13.8%). forty-one components representing 86% of the oil composition of anthemis scrobicular ...201830202228
the effect of permethrin resistance on aedes aegypti transcriptome following ingestion of zika virus infected blood.aedes aegypti (l.) is the primary vector of many emerging arboviruses. insecticide resistance among mosquito populations is a consequence of the application of insecticides for mosquito control. we used rna-sequencing to compare transcriptomes between permethrin resistant and susceptible strains of florida ae. aegypti in response to zika virus infection. a total of 2459 transcripts were expressed at significantly different levels between resistant and susceptible ae. aegypti. gene ontology analy ...201830200481
quiescence in aedes aegypti: interpopulation differences contribute to population dynamics and vectorial capacity.the strategy of aedes aegypti to prolong embryonic viability by quiescence has severe implications for geographic expansion and maintenance of mosquito populations in areas under control measures. we evaluated the effects of quiescence on biological parameters directly or indirectly associated with population dynamics and vectorial capacity in populations of this mosquito species from two brazilian municipalities characterized as dengue, chikungunya, and zika transmission areas. egg viability, i ...201830200417
nutritional quality during development alters insulin-like peptides' expression and physiology of the adult yellow fever mosquito, aedes aegypti.mosquitoes have distinct developmental and adult life history, and the vectorial capacity of females has been shown to be affected by the larval nutritional environment. however, little is known about the effect of developmental nutrition on insulin-signaling and nutrient storage. in this study, we used aedes aegypti, the yellow fever mosquito, to determine whether larval nutrition affects insulin gene expression. we also determined the traits regulated by insulin signaling, such as stored-nutri ...201830200185
evaluation of eosin-methylene blue as a photosensitizer for larval control of aedes aegypti by a photodynamic process.aedes aegypti (ae. aegypti) is a competent vector for transmitting important viral diseases such as yellow fever, dengue, chikungunya, and zika. several strategies have been applied to avoid ae. aegypti proliferation by using environmental management, biological, and chemical approaches. however, the development of new methods for effective control of the insect vector population is still needed. photodynamic control is an alternative way to control the vector population by using a physical appr ...201830200177
multiple insecticide resistance mechanisms in primary dengue vector, aedes aegypti (linn.) from dengue endemic districts of sub-himalayan west bengal, india.mosquitoes belonging to genus aedes are the prime vectors of several arboviral diseases such as dengue, zika and chikungunya worldwide. every year numerous cases of dengue infections occur throughout the world, proper control of which depends on efficient vector control. however the onset of insecticide resistance has resulted in failure of vector control approaches.201830199543
plant-derived compounds regulate formation of the insect juvenile hormone receptor complex.insect growth regulators (igrs) are attractive pest control agents due to their high target specificity and relative safety to the environment. recently, plants have been shown to synthesize igrs that affect the insect juvenile hormone (jh) as a part of their defense mechanisms. using a yeast two-hybrid system transformed with the aedes aegypti jh receptor as a reporter system, we identified several jh agonists (jhas) and antagonists (jhans) causing retardation in the ovarian development of fema ...201830195384
extensive recoding of dengue virus type 2 specifically reduces replication in primate cells without gain-of-function in aedes aegypti mosquitoes.dengue virus (denv), an arthropod-borne ("arbovirus") virus, causes a range of human maladies ranging from self-limiting dengue fever to the life-threatening dengue shock syndrome and proliferates well in two different taxa of the animal kingdom, mosquitoes and primates. mosquitoes and primates show taxonomic group-specific intolerance to certain codon pairs when expressing their genes by translation. this is called "codon pair bias". by necessity, dengue viruses evolved to delicately balance th ...201830192757
differential proteome analysis of chikungunya virus and dengue virus coinfection in aedes mosquitoes.chikungunya virus (chikv) and dengue virus (denv) are important arboviruses transmitted by aedes mosquitoes. these viruses are known to coexist within the same vector and coinfect the same host. although information is available on the mechanism of replication of chikv and denv when present independently in a vector, reports are lacking on the dynamics of virus-vector interactions when these viruses coexist in a mosquito. the current study attempts to understand the perturbations in the proteome ...201830192139
recent advances in threshold-dependent gene drives for mosquitoes.mosquito-borne diseases, such as malaria, dengue and chikungunya, cause morbidity and mortality around the world. recent advances in gene drives have produced control methods that could theoretically modify all populations of a disease vector, from a single release, making whole species less able to transmit pathogens. this ability has caused both excitement, at the prospect of global eradication of mosquito-borne diseases, and concern around safeguards. drive mechanisms that require individuals ...201830190331
evaluation of (-)-borneol derivatives against the zika vector, aedes aegypti and a non-target species, artemia sp.zika, dengue, and chikungunya are vector-borne diseases of pronounced concern transmitted by the mosquito aedes aegypti linn. (diptera: culicidae). the most important method to avoid outbreaks is to control mosquito spreading by the employment of insecticides and larvicides. failure to control mosquito dispersal is mostly accounted to ae. aegypti resistance to currently available larvicides and insecticides, encouraging the development of novel pesticides. in addition, the excessive use of larvi ...201830187415
knowledge, practices and entomological aspects of dengue in medellín, colombia: a comparative study of neighborhoods with high and low incidenceintroduction: dengue, mainly transmitted by aedes aegypti, is a very important viral disease in terms of public health. colombia is an endemoepidemic country for dengue and, in cities like medellín, there are neighborhoods with high and low incidence. the disease dynamics in the neighborhoods might be determined by differences in the knowledge, practices and entomological aspects of the vector among the communities.objective: to identify the knowledge and practices of residents of neighborhoods ...201830184371
novel larvicide tablets of bacillus thuringiensis var. israelensis: assessment of larvicidal effect on aedes aegypti (diptera: culicidae) in colombia.aedes (stegomyia) aegypti is the vector for dengue, chikungunya, and zika arboviruses. bti-cecif is a bioinsecticide designed and developed in the form of a solid tablet for the control of this vector. it contains bacillus thuringiensis var. israelensis (bti) serotype h-14.201830184370
exposure and sensitization to insects in allergic patients in the tropicsintroduction: mites are an important source of allergens in the tropics. other potential sources of allergens prevalent in the region such as insects have been poorly studied.objective: to determine the relationship between exposure and allergic sensitization to cockroaches, mosquitos, ants and the interaction with mite sensitization.materials and methods: we included patients with allergy tests for blatella germanica, aedes aegypti, solenopsis invicta, blomia tropicalis, dermatophagoides farina ...201830184366
genetic variability of aedes aegypti in the department of sucre, colombia, by analysis of the nucleotide sequence of the mitochondrial nd4 geneintroduction. aedes aegypti is the most important mosquito species in america for the transmission of viruses of dengue, zika, chikungunya and yellow fever. ecological factors as well as chemical controls can affect the genetic composition of ae. aegypti populations, which is why its genetic characterization is necessary.objective. to determine the genetic variability of ae. aegypti populations in four municipalities of sucre department, colombia.materials and methods. larvae of ae. aegypti, col ...201830184356
[first evidence of aedes aegypti in the arequipa region, perú 2016]. 201930183928
impedimetric immunosensor for dengue diagnosis using graphite screen-printed electrodes coated with poly(4-aminophenylacetic acid).dengue is an infectious viral disease transmitted by the aedes aegypti mosquito, the control of which is complex. in addition, the clinical diagnosis is difficult to perform since it resembles other febrile infections; thus, the development of more effective methods to detect dengue virus (dv) has drawn increasing attention. the present study aimed to develop an impedimetric immunosensor for dengue diagnosis using a screen-printed electrode (spe) functionalized with polymer films derived from 4- ...201830182296
dengue virus infection changes aedes aegypti oviposition olfactory preferences.aedes aegypti mosquitoes, main vectors for numerous flaviviruses, have olfactory preferences and are capable of olfactory learning especially when seeking their required environmental conditions to lay their eggs. in this study, we showed that semiochemical conditions during aedes aegypti larval rearing affected future female choice for oviposition: water-reared mosquitoes preferred to lay eggs in water or p-cresol containers, while skatole reared mosquitoes preferred skatole sites. using two in ...201830181545
a conceptual model for optimizing vaccine coverage to reduce vector-borne infections in the presence of antibody-dependent enhancement.many vector-borne diseases co-circulate, as the viruses from the same family are also transmitted by the same vector species. for example, zika and dengue viruses belong to the same flavivirus family and are primarily transmitted by a common mosquito species aedes aegypti. zika outbreaks have also commonly occurred in dengue-endemic areas, and co-circulation and co-infection of both viruses have been reported. as recent immunological cross-reactivity studies have confirmed that convalescent plas ...201830173664
demonstration of efficient vertical and venereal transmission of dengue virus type-2 in a genetically diverse laboratory strain of aedes aegypti.aedes aegypti is the primary mosquito vector of dengue viruses (denv; serotypes 1-4). human-mosquito transmission cycles maintain denv during epidemics but questions remain regarding how these viruses survive when human infections and vector abundance are minimal. aedes mosquitoes can transmit denv within the vector population through two alternate routes: vertical and venereal transmission (vt and vnt, respectively). we tested the efficiency of vt and vnt in a genetically diverse laboratory (gd ...201830169505
vertebrate hosts of aedes aegypti, aedes albopictus, and culex quinquefasciatus (diptera: culicidae) as potential vectors of zika virus in florida.zika virus (zikv), once considered an obscure pathogen, spread rapidly from 2014 to 2016 to become an internationally notifiable condition of major public health concern. the relative importance of various culex and aedes species mosquitoes (diptera: culicidae) in zikv transmission is a topic of debate. quantifying host use is important in determining the vectorial capacity of a mosquito species for transmitting zikv in nature. in the united states, few data are available on host use of aedes ae ...201930165498
differences in the replicative capacities of clinical isolates of dengue virus in c6/36 cells and in urban populations of aedes aegypti from colombia, south america.dengue, the most prevalent arboviral disease worldwide, is caused by any of the four dengue virus (denv) serotypes that co-circulate constantly in hyperendemic areas such as medellin (colombia), and these serotypes are transmitted by mosquitoes of the genus aedes. in this study, we evaluated the replicative capacity of strains isolated in medellin between 2003 and 2007 in c6/36 cells and in colonies of aedes aegypti collected during 2010-2011 from high or low-incidence areas within the same city ...201830165044
direct nucleic acid analysis of mosquitoes for high fidelity species identification and detection of wolbachia using a cellphone.manipulation of natural mosquito populations using the endosymbiotic bacteria wolbachia is being investigated as a novel strategy to reduce the burden of mosquito-borne viruses. to evaluate the efficacy of these interventions, it will be critical to determine wolbachia infection frequencies in aedes aegypti mosquito populations. however, current diagnostic tools are not well-suited to fit this need. morphological methods cannot identify wolbachia, immunoassays often suffer from low sensitivity a ...201830161131
characterization of tolypocladium cylindrosporum (hypocreales, ophiocordycipitaceae) isolates from brazil and their efficacy against aedes aegypti (diptera, culicidae).to survey and to characterize entomopathogenic fungi as natural enemies of mosquitoes in central brazil.201930160316
yellow fever in africa and the americas: a historical and epidemiological perspective.yellow fever was transported during the slave trade in the 15th and 16th centuries from africa to the americas where the virus encountered favorable ecological conditions that allowed creation of a sustainable sylvatic cycle. despite effective vector control and immunization programs for nearly a century, yellow fever epidemics reemerged in many latin american countries, particularly brazil. the emergence or reemergence of vector-borne diseases encompasses many intricate factors. yellow fever ou ...201830158957
local and regional dynamics of chikungunya virus transmission in colombia: the role of mismatched spatial heterogeneity.mathematical models of transmission dynamics are routinely fitted to epidemiological time series, which must inevitably be aggregated at some spatial scale. weekly case reports of chikungunya have been made available nationally for numerous countries in the western hemisphere since late 2013, and numerous models have made use of this data set for forecasting and inferential purposes. motivated by an abundance of literature suggesting that the transmission of this mosquito-borne pathogen is local ...201830157921
sublethal effects of a vapour-active pyrethroid, transfluthrin, on aedes aegypti and ae. albopictus (diptera: culicidae) fecundity and oviposition is assumed that mosquitoes surviving exposure to spatial repellents when attempting to bite a host will not have significant adverse impacts on their downstream biology. therefore, a critical knowledge gap is understanding the extent to which sublethal exposure to volatile pyrethroids may damage the performance of mosquitoes that survive exposure to vapour-active pyrethroids. to address this, laboratory-reared aedes aegypti (l.) and ae. albopictus (skuse) were exposed to one of three subletha ...201830157907
zika infection decreases aedes aegypti locomotor activity but does not influence egg production or viability.zika has emerged as a new public health threat after the explosive epidemic in brazil in 2015. it is an arbovirus transmitted mainly by aedes aegypti mosquitoes. the knowledge of physiological, behavioural and biological features in virus-infected vectors may help the understanding of arbovirus transmission dynamics and elucidate their influence in vector capacity.201830156598
oviposition deterrent efficacy and characteristics of a botanical natural product, ocimum gratissimum (l.) oil-alginate beads, against aedes aegypti (l.).this study was aimed at investigating the oviposition deterrent activity of ocimum gratissimum (l.) essential oil (o. gratissimum oil) and its product, ocimum gratissimum (l.)- alginate beads (beads), against aedes aegypti (ae. aegypti) mosquitoes. chemical analysis of o. gratissimum oil obtained by hydrodistillation, using gas chromatography-mass spectroscopy techniques, presented eugenol (67.38%) and z-β-ocimene (14.95 %) as major constituents. good characteristics of beads were obtained by th ...201830154681
forced salivation as a method to analyze vector competence of mosquitoes.vector competence is defined as the potential of a mosquito species to transmit a mosquito-borne virus (mobovirus) to a vertebrate host. viable virus particles are transmitted during a blood meal via the saliva of an infected mosquito. forced salivation assays allow determining the vector potential on the basis of single mosquitoes, avoiding the use of animal experiments. the method is suitable to analyze a large number of mosquitoes in one experiment within a short period of time. forced saliva ...201830148488
qsar modelling of synergists to increase the efficacy of deltamethrin against pyrethroid-resistant aedes aegypti mosquitoes$.space spraying of deltamethrin allows the control of adult aedes (stegomyia) aegypti mosquitoes. unfortunately, many vector control programs are threatened by the development of resistances that decrease the efficacy of this adulticide. faced with this situation, we can either try to use another insecticide presenting a different mechanism of action or find a strategy that brings back the efficacy of the insecticide at a satisfying level to pursue its use in vector control. restoration of the ef ...201830141356
transcriptomic analysis of aedes aegypti in response to mosquitocidal bacillus thuringiensis llp29 toxin.globally, aedes aegypti is one of the most dangerous mosquitoes that plays a crucial role as a vector for human diseases, such as yellow fever, dengue, and chikungunya. to identify (1) transcriptomic basis of midgut (2) key genes that are involved in the toxicity process by a comparative transcriptomic analysis between the control and bacillus thuringiensis (bt) toxin (llp29 proteins)-treated groups. next-generation sequencing technology was used to sequence the midgut transcriptome of a. aegypt ...201830140020
estimating the size of aedes aegypti populations from dengue incidence data: implications for the risk of yellow fever this paper we present a model to estimate the density of aedes mosquitoes in a community affected by dengue. the method consists in fitting a continuous function to the incidence of dengue infections, from which the density of infected mosquitoes is derived straightforwardly. further derivations allow the calculation of the latent and susceptible mosquitoes' densities, the sum of the three equals the total mosquitoes' density. the method is illustrated with the case of the risk of urban yello ...201730137722
evaluation of standard field and laboratory methods to compare protection times of the topical repellents pmd and deet.mosquitoes are important vectors of pathogens, and travellers to disease endemic countries are advised to avoid bites by applying topical repellents. topical repellents are typically tested either in the arm-in-cage (aic) test under laboratory conditions or in the field, but not often under both conditions. we, therefore, investigated how two topical repellents, 15% para-menthane-3,8-diol (pmd) and 15% n,n-diethyl-3-methylbenzamide (deet) compare against each other both in the aic test against t ...201830135603
experimental evaluation of the impact of household aerosolized insecticides on pyrethroid resistant aedes aegypti.the extensive reliance on insecticides to control aedes aegypti mosquitoes and disrupt transmission of dengue, chikungunya and zika has fueled the emergence of widespread resistance to insecticides. mismatch between the frequency of pyrethroid resistance in mosquitoes and the occurrence of pyrethroid-based insecticide applications for vector control is often hypothesized to be due to household use of commercial insecticide products. we experimentally quantified phenotypic and genotypic responses ...201830135460
differential transmission of antiviral drug-resistant chikungunya viruses by aedes mosquitoes.the chikungunya virus (chikv) is transmitted by female aedes aegypti and aedes albopictus mosquitoes, mostly present in (sub)tropical regions. no antivirals are available to treat chikv infections. if antiviral drugs are proven efficient to treat chikv-infected patients, it will be pivotal to determine whether drug-resistant viruses can be transmitted from one human to another by their mosquito vectors. we orally infected aedes aegypti mosquitoes with a blood meal containing wild-type or drug-re ...201830135220
study of aedes aegypti population with emphasis on the gonotrophic cycle length and identification of arboviruses: implications for vector management in cemeteries.aedes aegypti is the vector of the arboviruses causing dengue, chikungunya and zika infections in mexico. however, its presence in public places has not been fully evaluated. in a cemetery from merida, yucatan, mexico, the productivity of ae. aegypti, the gonotrophic cycle, and the presence of ae. aegypti females infected with arboviruses were evaluated. immature and adult mosquitoes were inspected every two months between april 2016 to june 2017. for the gonotrophic cycle length, the daily patt ...201830133604
estimating the effects of variation in viremia on mosquito susceptibility, infectiousness, and r0 of zika in aedes aegypti.zika virus (zikv) is an arbovirus primarily transmitted by aedes mosquitoes. like most viral infections, zikv viremia varies over several orders of magnitude, with unknown consequences for transmission. to determine the effect of viral concentration on zikv transmission risk, we exposed field-derived ae. aegypti mosquitoes to four doses (10(3), 10(4), 10(5), 10(6) pfu/ml) representative of potential variation in the field. we demonstrate that increasing zikv dose in the blood-meal significantly ...201830133450
estimating air temperature using modis surface temperature images for assessing aedes aegypti thermal niche in bangkok, thailand.dengue, the most widespread urban vector-borne disease, is transmitted to human by the mosquito aedes aegypti. its distribution in urban areas is heterogeneous over time and space. in time, it is linked to seasonal variations such as warm and cold seasons, as well as rainy and dry seasons. in space, it is linked to social and environmental conditions, which alternate between rich and deprived neighborhoods, vegetated and densely built areas. these variations in terms of land cover can affect sur ...201830132225
efficacy of culicinomyces spp. against aedes aegypti eggs, larvae and adults.the aquatic fungal genus culicinomyces attacks dipteran larvae but little is known about its efficacy against aedes aegypti. here we report on the activity of both described species-culicinomyces clavisporus and culicinomyces bisporalis-on larvae, eggs and adults, and on trans-stadial transmission. ten c. clavisporus isolates (arsef 372, 582, 644, 706, 964, 1260, 2471, 2478, 2479 and 2480) and c. bisporalis arsef 1948 were screened against larvae of this important vector of viral diseases. arsef ...201830130538
glyphosate impairs learning in aedes aegypti mosquito larvae at field-realistic doses.glyphosate is the most widely used herbicide in the world. over the past few years, the number of studies revealing deleterious effects of glyphosate on non-target species has been increasing. here, we studied the impact of glyphosate at field-realistic doses on learning in mosquito larvae (aedes aegypti). larvae of a. aegypti live in small bodies of water and perform a stereotyped escape response when a moving object projects its shadow on the water surface. repeated presentations of an innocuo ...201830127074
laboratory selection of aedes aegypti field populations with the organophosphate malathion: negative impacts on resistance to deltamethrin and to the organophosphate temephos.resistance to pyrethroids and to the organophosphate temephos is widespread in brazilian populations of the dengue vector, aedes aegypti. thereof, since 2009 insect growth regulators are employed as larvicides, and malathion is used against adults.201830125295
travel-associated zika cases and threat of local transmission during global outbreak, california, usa.zika and associated microcephaly among newborns were reported in brazil during 2015. zika has since spread across the americas, and travel-associated cases were reported throughout the united states. we reviewed travel-associated zika cases in california to assess the potential threat of local zika virus transmission, given the regional spread of aedes aegypti and ae. albopictus mosquitoes. during november 2015-september 2017, a total of 588 travel-associated zika cases were reported in californ ...201830124194
chicory (cichorium intybus) and wormwood (artemisia absinthium) extracts exhibit strong larvicidal activity against mosquito vectors of malaria, dengue fever, and filariasis.vector-borne diseases transmitted by mosquitoes cause globally important diseases such as malaria, dengue fever, and filariasis. the incidence of these diseases can be reduced through mosquito control programs but these control programs currently rely on synthetic insecticides that can impact the environment, and has selected widespread mosquito resistance. environment friendly and biodegradable natural insecticides discovered in plants offer an alternative approach to mosquito control. here, we ...201830118862
colonized sabethes cyaneus, a sylvatic new world mosquito species, shows a low vector competence for zika virus relative to aedes aegypti.the introduction of zika virus (zikv) to the americas raised concern that the virus would spill back from human transmission, perpetuated by aedes aegypti, into a sylvatic cycle maintained in wildlife and forest-living mosquitoes. in the americas, sabethes species are vectors of sylvatic yellow fever virus (yfv) and are therefore candidate vectors of a sylvatic zikv cycle. to test the potential of sabethes cyaneus to transmit zikv, sa. cyaneus and ae. aegypti were fed on a129 mice one or two day ...201830115888
levels of resistance to pyrethroid among distinct kdr alleles in aedes aegypti laboratory lines and frequency of kdr alleles in 27 natural populations from rio de janeiro, brazil.several mutations in voltage gated sodium channel (nav) have been identified in aedes aegypti populations worldwide. however, only few are related to knockdown resistance to pyrethroids, most of which with variations in the 1016 and 1534 nav sites. in brazil, at least two nav alleles are known: navr1, with a substitution in the 1534 (1016 val+ + 1534 ile kdr ) and navr2, with substitutions in both 1016 and sites (1016ilekdr + 1534cys kdr ). there is also the duplication in the nav gene, with one ...201830112367
oviplate: a convenient and space-saving method to perform individual oviposition assays in aedes aegypti.aedes aegypti is the principal vector of the urban arboviruses and the blood ingestion is important to produce the eggs in this species. to analyze the egg production in ae. aegypti, researchers frequently use small cages or drosophila vials to collect eggs from gravid females. although it is affordable, the setup is time- and space-consuming, mainly when many mosquitoes need to be individually analyzed. this study presents an easy, cheap, and space-saving method to perform individual ovipositio ...201830111702
temperature drives zika virus transmission: evidence from empirical and mathematical models.temperature is a strong driver of vector-borne disease transmission. yet, for emerging arboviruses we lack fundamental knowledge on the relationship between transmission and temperature. current models rely on the untested assumption that zika virus responds similarly to dengue virus, potentially limiting our ability to accurately predict the spread of zika. we conducted experiments to estimate the thermal performance of zika virus (zikv) in field-derived aedes aegypti across eight constant temp ...201830111605
zika might not be acting alone: using an ecological study approach to investigate potential co-acting risk factors for an unusual pattern of microcephaly in brazil.zika virus infections can cause a range of neurologic disorders including congenital microcephaly. however, while zika infections have been notified across all regions in brazil, there has been an unusual number of congenital microcephaly case notifications concentrated in the northeast of the country. to address this observation, we investigated epidemiological data (2014-2016) on arbovirus co-distribution, environmental and socio-economic factors for each region in brazil. data on arbovirus re ...201830110370
parental and offspring larval diets interact to influence life-history traits and infection with dengue virus in aedes aegypti.the environmental conditions experienced by parents can influence offspring phenotype along with the conditions experienced by offspring. these parental effects are clear in organisms that display parental care and are less clear in other organisms. here, we consider effects of parental and offspring larval nutrition on offspring development time, survivorship and infection with dengue virus in aedes aegypti, the mosquito vector of dengue, chikungunya, yellow fever and zika. parents were raised ...201830109101
anti-inflammatory activities of aedes aegypti cecropins and their protection against murine endotoxin shock.mosquitoes are armed with physiologically active compounds to suppress the host immunity including host inflammatory reaction. however, the specific anti-inflammatory components in mosquitoes remain unknown.201830107813
evaluation of aib and peg-polymer insect kinin analogs on mosquito and tick gpcrs identifies potent new pest management tools with potentially enhanced biostability and bioavailability.insect kinins modulate aspects of diuresis, digestion, development, and sugar taste perception in tarsi and labellar sensilla in mosquitoes. they are, however, subject to rapid biological degradation by endogenous invertebrate peptidases. a series of α-aminoisobutyric (aib) acid-containing insect kinin analogs incorporating sequences native to the aedes aegypti mosquito aedeskinins were evaluated on two recombinant kinin invertebrate receptors stably expressed in cell lines, discovering a number ...201930107140
increased adult aedes aegypti and culex quinquefasciatus (diptera: culicidae) abundance in a dengue transmission hotspot, compared to a coldspot, within kaohsiung city, taiwan.the assumption that vector abundance differences might drive spatial and temporal heterogeneities in vector-borne disease transmission is common, though data supporting it is scarce. here, we present data from two common mosquito species aedes aegypti (linnaeus) and culex quinquefasciatus say, biweekly sampled as adults, from march 2016 through december 2017, with bg-sentinel traps in two neighboring districts of kaohsiung city (kc), taiwan. one district has historically been a dengue transmissi ...201830104501
impact of mosquito age and insecticide exposure on susceptibility of aedes albopictus (diptera: culicidae) to infection with zika virus.zika virus (zikv) is primarily transmitted to humans by aedes aegypti and ae. albopictus. vector⁻virus interactions influencing vector competence vary and depend on biological and environmental factors. a mosquito's chronological age may impact its immune response against virus infection. insecticides, source reduction, and/or public education are currently the best defense against mosquitoes that transmit zikv. this study assessed the effects of a mosquito's chronological age at time of infecti ...201830103526
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